Rh4AG025200

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0009431
Physical Location & Seq
Forward (+)
4864251 .. 4869350
5100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG025200.1

Sequence Viewer

Length: 1356 bp
ATGAACGTCGCCAGGCTCAACATGTGCCACGGCACGCGCGACTGGCACCGCGCGGTGATTGAGCGCGTGAGGAAGCTCAACGAGGACAAGGGCTACGCCGTCGCGATTATGATGGATACGGAAGGGAGTGAGATTCACATGGGCGATCTCGGCACTTCCTCCGTCAAAGCTGAGGATGGTGAGATATGGACTTTCAGTGTCAGAGCGTTCGGTTCTACTCTTCCGGAACGCACCATCAATGTGAATTACGAGGGTTTCGCCGAAGATGTGAAAGTGGGCGATGAGCTACTTGTGGATGGTGGAATGGTGAGGTTTGAGGTGGTTGAGAAGCTTGGCCCGGATGTCAAGTGTAAATGTACTGATCCCGGACTGTTGCTGCCGCGGGCTAACTTGACTTTCTGGAGGGATGGGAGTTTAGTGCGGGAACGAAATGCCATGCTTCCCACAATTTCTTCCAAGGATTGGTTGGACATTGATTTCGGGATTGCTGAAGGTGTTGATTTCATTGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCACCTTAAAAGCTATATTGCAGCACGGTCTCGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATTGTTGCTTCTCAACTACTCGAATCTATGATCGAGTACCCTACACCCACTAGAGCTGAAGTGGCCGATGTTTCTGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAACCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGCAAATAATTTAGAGGTGGATGCTCTTTTCATCTTTACAAAGACGGGCCACATGGCATCTCTCCTGTCGCGCTGCCGGCCTGACTGCCCAATCTTTGCTTTTACCAACACAACATCTGTGCGGAGACGTCTGAACCTACAGTGGGGGCTGATACCATTTCGTGTTAGTTTCTCTGATGATATGGAAAGCAACCTTCACAAGACCTTCTCACTGCTGAAGGCCAGGAATCTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATATTGCAGTCCATTCAAGTTATGAATGTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

451

Amino Acids

50.09

Weight (kDa)

5.49

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 1 - 303 1.7e-80 Pyruvate kinase, barrel domain
PK_C PF02887 342 - 441 1.5e-23 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1183
AccB1I GGYRCC 1 cut(s) 45
AccB7I CCANNNNNTGG 2 cut(s) 462, 995
AccII CGCG 9 cut(s) 37, 39, 51, 53, 66, 104, 382, 576, 1123
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 6 cut(s) 49, 53, 380, 382, 421, 1174
AclWI GGATC 3 cut(s) 356, 947, 1006
AcoI YGGCCR 1 cut(s) 822
AcuI CTGAAG 5 cut(s) 510, 552, 837, 855, 1289
AcyI GRCGYC 1 cut(s) 1180
AfaI GTAC 3 cut(s) 358, 797, 1350
AfiI CCNNNNNNNGG 3 cut(s) 462, 995, 1195
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 2 cut(s) 731, 1337
AjnI CCWGG 2 cut(s) 11, 1274
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 3 cut(s) 576, 1171, 1318
AlwI GGATC 3 cut(s) 356, 947, 1006
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 6 cut(s) 334, 822, 889, 1099, 1130, 1272
ApeKI GCWGC 6 cut(s) 376, 563, 742, 847, 1043, 1125
ArsI GACNNNNNNTTYG 2 cut(s) 461, 493
AspA2I CCTAGG 1 cut(s) 676
AspLEI GCGC 4 cut(s) 39, 53, 66, 1125
AspS9I GGNCC 4 cut(s) 335, 703, 889, 1099
AsuC2I CCSGG 2 cut(s) 338, 366
AsuHPI GGTGA 7 cut(s) 67, 191, 319, 536, 683, 887, 1307
AvaII GGWCC 1 cut(s) 703
AvrII CCTAGG 1 cut(s) 676
BanI GGYRCC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 685
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 6 cut(s) 363, 575, 754, 859, 1030, 1112
BccI CCATC 8 cut(s) 106, 170, 242, 290, 401, 644, 715, 1045
BceAI ACGGC 3 cut(s) 46, 83, 824
BciT130I CCWGG 2 cut(s) 13, 1276
BciVI GTATCC 1 cut(s) 109
BclI TGATCA 2 cut(s) 537, 1284
BcnI CCSGG 2 cut(s) 338, 366
BcoDI GTCTC 3 cut(s) 576, 1171, 1318
BfaI CTAG 2 cut(s) 677, 810
BfmI CTRYAG 1 cut(s) 1190
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 1052
BisI GCNGC 7 cut(s) 377, 380, 564, 743, 848, 1044, 1126
BlnI CCTAGG 1 cut(s) 676
BlsI GCNGC 7 cut(s) 378, 381, 565, 744, 849, 1045, 1127
Bme1390I CCNGG 4 cut(s) 13, 338, 366, 1276
Bme18I GGWCC 1 cut(s) 703
BmgT120I GGNCC 4 cut(s) 335, 703, 889, 1099
BmiI GGNNCC 2 cut(s) 47, 705
BmrFI CCNGG 4 cut(s) 13, 338, 366, 1276
BmsI GCATC 5 cut(s) 653, 847, 855, 1063, 1118
BpmI CTGGAG 1 cut(s) 421
Bpu10I CCTNAGC 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 338, 366
BsaHI GRCGYC 1 cut(s) 1180
BsaI GGTCTC 1 cut(s) 576
BsaJI CCNNGG 4 cut(s) 28, 380, 456, 676
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bsc4I CCNNNNNNNGG 3 cut(s) 462, 995, 1195
Bse118I RCCGGY 1 cut(s) 1128
Bse1I ACTGG 3 cut(s) 47, 755, 892
Bse3DI GCAATG 3 cut(s) 504, 663, 1305
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 2 cut(s) 13, 1276
BseDI CCNNGG 4 cut(s) 28, 380, 456, 676
BseGI GGATG 6 cut(s) 181, 301, 346, 412, 636, 1078
BseLI CCNNNNNNNGG 3 cut(s) 462, 995, 1195
BseMI GCAATG 3 cut(s) 504, 663, 1305
BseMII CTCAG 4 cut(s) 162, 698, 934, 1329
BseNI ACTGG 3 cut(s) 47, 755, 892
BseRI GAGGAG 1 cut(s) 1040
BseXI GCAGC 6 cut(s) 363, 575, 754, 859, 1030, 1112
BseYI CCCAGC 2 cut(s) 714, 988
Bsh1236I CGCG 9 cut(s) 37, 39, 51, 53, 66, 104, 382, 576, 1123
BshFI GGCC 6 cut(s) 336, 824, 891, 1101, 1132, 1274
BshNI GGYRCC 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 4 cut(s) 224, 338, 366, 1129
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 3 cut(s) 462, 995, 1195
BsmAI GTCTC 3 cut(s) 576, 1171, 1318
BsmBI CGTCTC 1 cut(s) 1171
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 6 cut(s) 336, 824, 891, 1101, 1132, 1274
Bso31I GGTCTC 1 cut(s) 576
Bsp1286I GDGCHC 1 cut(s) 685
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
Bsp68I TCGCGA 2 cut(s) 104, 576
BspACI CCGC 6 cut(s) 49, 53, 380, 382, 421, 1174
BspANI GGCC 6 cut(s) 336, 824, 891, 1101, 1132, 1274
BspCNI CTCAG 4 cut(s) 163, 697, 933, 1328
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 9 cut(s) 37, 39, 51, 53, 66, 104, 382, 576, 1123
BspHI TCATGA 1 cut(s) 970
BspLI GGNNCC 2 cut(s) 47, 705
BspPI GGATC 3 cut(s) 356, 947, 1006
BspT107I GGYRCC 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 576
BsrDI GCAATG 3 cut(s) 504, 663, 1305
BsrFI RCCGGY 1 cut(s) 1128
BsrI ACTGG 3 cut(s) 47, 755, 892
BssAI RCCGGY 1 cut(s) 1128
BssECI CCNNGG 4 cut(s) 28, 380, 456, 676
BssMI GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
BssNI GRCGYC 1 cut(s) 1180
BssT1I CCWWGG 2 cut(s) 456, 676
Bst2UI CCWGG 2 cut(s) 13, 1276
Bst4CI ACNGT 5 cut(s) 372, 570, 1016, 1194, 1353
Bst6I CTCTTC 3 cut(s) 225, 624, 954
BstACI GRCGYC 1 cut(s) 1180
BstC8I GCNNGC 4 cut(s) 35, 384, 852, 1130
BstDEI CTNAG 4 cut(s) 171, 684, 920, 1315
BstDSI CCRYGG 2 cut(s) 28, 380
BstEII GGTNACC 2 cut(s) 671, 1295
BstF5I GGATG 6 cut(s) 181, 301, 346, 412, 636, 1078
BstFNI CGCG 9 cut(s) 37, 39, 51, 53, 66, 104, 382, 576, 1123
BstHHI GCGC 4 cut(s) 39, 53, 66, 1125
BstKTI GATC 8 cut(s) 148, 364, 540, 636, 792, 942, 1001, 1287
BstMAI GTCTC 3 cut(s) 576, 1171, 1318
BstMBI GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
BstMWI GCNNNNNNNGC 7 cut(s) 43, 150, 662, 751, 821, 1052, 1129
BstNI CCWGG 2 cut(s) 13, 1276
BstNSI RCATGY 1 cut(s) 25
BstPI GGTNACC 2 cut(s) 671, 1295
BstSCI CCNGG 4 cut(s) 11, 336, 364, 1274
BstSFI CTRYAG 1 cut(s) 1190
BstUI CGCG 9 cut(s) 37, 39, 51, 53, 66, 104, 382, 576, 1123
BstV1I GCAGC 6 cut(s) 363, 575, 754, 859, 1030, 1112
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 6 cut(s) 336, 824, 891, 1101, 1132, 1274
BtgI CCRYGG 2 cut(s) 28, 380
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 6 cut(s) 181, 301, 346, 412, 636, 1078
BtsI GCAGTG 1 cut(s) 1262
BtsIMutI CAGTG 3 cut(s) 202, 1199, 1262
BtuMI TCGCGA 2 cut(s) 104, 576
Cac8I GCNNGC 4 cut(s) 35, 384, 852, 1130
CciI TCATGA 1 cut(s) 970
CfoI GCGC 4 cut(s) 39, 53, 66, 1125
Cfr10I RCCGGY 1 cut(s) 1128
Cfr13I GGNCC 4 cut(s) 335, 703, 889, 1099
Cfr42I CCGCGG 1 cut(s) 383
Csp6I GTAC 3 cut(s) 357, 796, 1349
CviAII CATG 5 cut(s) 22, 139, 436, 971, 1105
CviQI GTAC 3 cut(s) 357, 796, 1349
DdeI CTNAG 4 cut(s) 171, 684, 920, 1315
DpnI GATC 8 cut(s) 147, 363, 539, 635, 791, 941, 1000, 1286
DpnII GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
EaeI YGGCCR 1 cut(s) 822
Eam1104I CTCTTC 3 cut(s) 225, 624, 954
EarI CTCTTC 3 cut(s) 225, 624, 954
Eco130I CCWWGG 2 cut(s) 456, 676
Eco31I GGTCTC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 703
Eco57I CTGAAG 5 cut(s) 510, 552, 837, 855, 1289
Eco91I GGTNACC 2 cut(s) 671, 1295
EcoO109I RGGNCCY 1 cut(s) 703
EcoO65I GGTNACC 2 cut(s) 671, 1295
EcoRII CCWGG 2 cut(s) 11, 1274
EcoT14I CCWWGG 2 cut(s) 456, 676
ErhI CCWWGG 2 cut(s) 456, 676
Esp3I CGTCTC 1 cut(s) 1171
FaeI CATG 5 cut(s) 25, 142, 439, 974, 1108
FaqI GGGAC 1 cut(s) 689
FatI CATG 5 cut(s) 21, 138, 435, 970, 1104
FauI CCCGC 2 cut(s) 375, 414
FbaI TGATCA 2 cut(s) 537, 1284
Fnu4HI GCNGC 7 cut(s) 377, 380, 564, 743, 848, 1044, 1126
FokI GGATG 6 cut(s) 188, 308, 353, 419, 623, 1085
Fsp4HI GCNGC 7 cut(s) 377, 380, 564, 743, 848, 1044, 1126
FspBI CTAG 2 cut(s) 677, 810
GlaI GCGC 4 cut(s) 38, 52, 65, 1124
GluI GCNGC 7 cut(s) 377, 380, 564, 743, 848, 1044, 1126
GsaI CCCAGC 2 cut(s) 718, 992
GsuI CTGGAG 1 cut(s) 421
HaeIII GGCC 6 cut(s) 336, 824, 891, 1101, 1132, 1274
HapII CCGG 4 cut(s) 224, 338, 366, 1129
HhaI GCGC 4 cut(s) 39, 53, 66, 1125
Hin1I GRCGYC 1 cut(s) 1180
Hin1II CATG 5 cut(s) 25, 142, 439, 974, 1108
Hin6I GCGC 4 cut(s) 37, 51, 64, 1123
HinP1I GCGC 4 cut(s) 37, 51, 64, 1123
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 5 cut(s) 133, 611, 782, 878, 1279
HpaII CCGG 4 cut(s) 224, 338, 366, 1129
HphI GGTGA 7 cut(s) 67, 191, 319, 536, 683, 887, 1307
Hpy188III TCNNGA 7 cut(s) 103, 224, 400, 481, 575, 943, 971
Hpy99I CGWCG 2 cut(s) 11, 104
HpyAV CCTTC 5 cut(s) 116, 485, 1256, 1264, 1267
HpyCH4III ACNGT 5 cut(s) 372, 570, 1016, 1194, 1353
HpyCH4IV ACGT 3 cut(s) 6, 967, 1180
HpyCH4V TGCA 3 cut(s) 563, 644, 1327
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 150, 662, 751, 821, 1052, 1129
HpyF3I CTNAG 4 cut(s) 171, 684, 920, 1315
HpySE526I ACGT 3 cut(s) 6, 967, 1180
Hsp92I GRCGYC 1 cut(s) 1180
Hsp92II CATG 5 cut(s) 25, 142, 439, 974, 1108
HspAI GCGC 4 cut(s) 37, 51, 64, 1123
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 1128
KroNI GCCGGC 1 cut(s) 1130
Ksp22I TGATCA 2 cut(s) 537, 1284
KspI CCGCGG 1 cut(s) 383
Kzo9I GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
LmnI GCTCC 2 cut(s) 653, 1027
Lsp1109I GCAGC 6 cut(s) 363, 575, 754, 859, 1030, 1112
LweI GCATC 5 cut(s) 653, 847, 855, 1063, 1118
MaeI CTAG 2 cut(s) 677, 810
MaeII ACGT 3 cut(s) 6, 967, 1180
MaeIII GTNAC 3 cut(s) 671, 981, 1295
MalI GATC 8 cut(s) 147, 363, 539, 635, 791, 941, 1000, 1286
MboI GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
MboII GAAGA 5 cut(s) 212, 275, 444, 641, 971
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 3 cut(s) 244, 447, 1060
MlyI GAGTC 2 cut(s) 605, 887
MmeI TCCRAC 1 cut(s) 447
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 1128
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 239
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 4 cut(s) 224, 338, 366, 1129
MspR9I CCNGG 4 cut(s) 13, 338, 366, 1276
MvaI CCWGG 2 cut(s) 13, 1276
MvnI CGCG 9 cut(s) 37, 39, 51, 53, 66, 104, 382, 576, 1123
MwoI GCNNNNNNNGC 7 cut(s) 43, 150, 662, 751, 821, 1052, 1129
NaeI GCCGGC 1 cut(s) 1130
NciI CCSGG 2 cut(s) 338, 366
NdeII GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
NgoMIV GCCGGC 1 cut(s) 1128
NlaIII CATG 5 cut(s) 25, 142, 439, 974, 1108
NlaIV GGNNCC 2 cut(s) 47, 705
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 2 cut(s) 671, 1295
NruI TCGCGA 2 cut(s) 104, 576
NspI RCATGY 1 cut(s) 25
PagI TCATGA 1 cut(s) 970
PciI ACATGT 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdiI GCCGGC 1 cut(s) 1130
PfeI GAWTC 3 cut(s) 133, 782, 1279
PflFI GACNNNGTC 1 cut(s) 1301
PflMI CCANNNNNTGG 2 cut(s) 462, 995
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 7 cut(s) 378, 381, 565, 744, 849, 1045, 1127
PleI GAGTC 2 cut(s) 605, 886
PpsI GAGTC 2 cut(s) 605, 886
PpuMI RGGWCCY 1 cut(s) 703
PscI ACATGT 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 703
Psp6I CCWGG 2 cut(s) 11, 1274
PspEI GGTNACC 2 cut(s) 671, 1295
PspFI CCCAGC 2 cut(s) 714, 988
PspGI CCWGG 2 cut(s) 11, 1274
PspN4I GGNNCC 2 cut(s) 47, 705
PspPI GGNCC 4 cut(s) 335, 703, 889, 1099
PspPPI RGGWCCY 1 cut(s) 703
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PsyI GACNNNGTC 1 cut(s) 1301
RruI TCGCGA 2 cut(s) 104, 576
RsaI GTAC 3 cut(s) 358, 797, 1350
RsaNI GTAC 3 cut(s) 357, 796, 1349
RseI CAYNNNNRTG 1 cut(s) 239
SacII CCGCGG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 7 cut(s) 377, 380, 564, 743, 848, 1044, 1126
Sau3AI GATC 8 cut(s) 145, 361, 537, 633, 789, 939, 998, 1284
Sau96I GGNCC 4 cut(s) 335, 703, 889, 1099
SchI GAGTC 2 cut(s) 605, 887
ScrFI CCNGG 4 cut(s) 13, 338, 366, 1276
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 5 cut(s) 653, 847, 855, 1063, 1118
SfcI CTRYAG 1 cut(s) 1190
Sfr303I CCGCGG 1 cut(s) 383
SgrBI CCGCGG 1 cut(s) 383
SinI GGWCC 1 cut(s) 703
SmiMI CAYNNNNRTG 1 cut(s) 239
Sse9I AATT 3 cut(s) 244, 447, 1060
SsiI CCGC 6 cut(s) 49, 53, 380, 382, 421, 1174
SspMI CTAG 2 cut(s) 677, 810
StyD4I CCNGG 4 cut(s) 11, 336, 364, 1274
StyI CCWWGG 2 cut(s) 456, 676
TaaI ACNGT 5 cut(s) 372, 570, 1016, 1194, 1353
TaiI ACGT 3 cut(s) 9, 970, 1183
TaqI TCGA 3 cut(s) 780, 792, 942
TasI AATT 3 cut(s) 244, 447, 1060
TatI WGTACW 1 cut(s) 356
TauI GCSGC 1 cut(s) 382
TfiI GAWTC 3 cut(s) 133, 782, 1279
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 3 cut(s) 202, 1199, 1269
TseFI GTSAC 2 cut(s) 671, 1295
TseI GCWGC 6 cut(s) 376, 563, 742, 847, 1043, 1125
Tsp45I GTSAC 2 cut(s) 671, 1295
TspDTI ATGAA 4 cut(s) 17, 493, 987, 1072
TspGWI ACGGA 2 cut(s) 134, 151
TspRI CASTG 3 cut(s) 202, 1199, 1269
Tth111I GACNNNGTC 1 cut(s) 1301
Van91I CCANNNNNTGG 2 cut(s) 462, 995
VpaK11BI GGWCC 1 cut(s) 703
XceI RCATGY 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmaJI CCTAGG 1 cut(s) 676
XspI CTAG 2 cut(s) 677, 810
ZraI GACGTC 1 cut(s) 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.