Rh4CG028200

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009431
Physical Location & Seq
Forward (+)
5309298 .. 5316105
6808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG028200.1

Sequence Viewer

Length: 1473 bp
ATGTCTCAGTCCCTTCACCTCCTCACCCCCACCACCTTAACCTTCCCCAAACCTCATTCCTCCTCCTCCTCCTCCTCCTCATTCCTCAACCCCTTCTCCACCCCCTATCGCCGGTTTCCGGTCACCACCGGCTTCCCCACCTTCTCCATCAAAGCCTCCGCCGCGGATCAAAAACCCTCCGTGCTCGTCTCCGGCAACGGCACCTCCTCCTCCGCGGTACAATCGCAGCAGGACTATGCTTCCTCCACCTCCATCGAGGTCGACGCCGTCACCGAGGCCGAGCTCCGGGAGAACGGCTTCCGAAGCACGCGGCGGACGAAGCTCGTCTGCACCATCGGCCCCGCCACCGCCGGCTTCGACCAGCTCGAGGCCCTCGCCGTCGGCGGCATGAACGTCGCCAGGCTCAACATGTGCCACGGCACGCGCGACTGGCACCGCGCGGTGATTGAGCGCGTGAGGAAGCTCAACGAGGACAAGGGCTACGCCGTCGCGATTATGATGGATACCGAAGGGAGTGAGATTCACATGGGCGATCTCGGCACTTCCTCCGTCAAAGCCGAGGATGGTGAGATATGGACTTTCAGTGTCAGAGCTTTCGGTTCTACCCTTCCGGAACGCACCATCAATGTGAATTACGAGGGTTTCGCCGAAGATGTGAAAGTGGGCGATGAGCTACTTGTGGATGGTGGAATGGTGAGGTTTGAGGTGGTTGAGAAGCTTGGCCCGGATGTCAAGTGTAAATGTACTGATCCCGGACTGTTGCTGCCGCGGGCTAACTTGACTTTCTGGAGGGATGGGAGTTTAGTGCGAGAACGAAATGCCATGCTTCCCACAATTTCTTCAAAGGATTGGTTGGACATTGATTTCGGGATTGCTGAGGGTGTTGATTTCATCGCTGTATCTTTTGTCAAGTCTGCTGAAGTGATCAATCACCTTAAAAGCTATATTGCAGCACGGTCTAGCGATATTTCTGTTGTGGCAAAGATAGAGAGTATTGACTCTCTAAAGAACTTGGAAGAGATCATCCTTGCATCAGATGGAGCAATGGTAGCAAGAGGTGACCTAGGTGCTCAGATACCATTGGAACAGGTCCCATCAGCCCAGCAAAAGATTGTTCAAGTATGTCGGCAGCTAAATAAGCCAGTCATCGTTGCTTCTCAACTACTCGAATCTATGATCGAGTATCCTACACCCACTAGAGCTGAAGTGGCAGATGTTTCGGAAGCCGTGAGGCAGCGAGCTGATGCTCTGATGCTTTCTGGTGAGTCAGCTATGGGCCAGTTCCCAGATAAGTCATTAGCTGTTCTCAGAAGTGTCAGTTTGAGGATCGAGAGGTGGTGGAGGGAAGAGAAACGTCATGAATCTATGTTACTCCCAGCTATTGGATCATCATTTTCAGACAGTATATCGGAGGAGCTTTGTATTTCTGCTGCCAAGATGGGTAAGCATTCTTTGATTGACATCTGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000287 GO:0003006 GO:0003674 GO:0003824 GO:0004743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006629 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009791 GO:0009987 GO:0010035 GO:0010038 GO:0010154 GO:0010431 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0021700 GO:0022414 GO:0030955 GO:0031420 GO:0032501 GO:0032502 GO:0032504 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042221 GO:0042866 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046686 GO:0046700 GO:0046872 GO:0046939 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0061458 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

490

Amino Acids

53.46

Weight (kDa)

5.33

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 104 - 432 5.7e-91 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 200, 432
AccB7I CCANNNNNTGG 1 cut(s) 1382
AccI GTMKAC 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 610
AclWI GGATC 4 cut(s) 174, 743, 1334, 1393
AcuI CTGAAG 2 cut(s) 939, 1224
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 219, 745
AfiI CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 1382
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 2 cut(s) 843, 1118
AjnI CCWGG 1 cut(s) 398
Alw21I GWGCWC 3 cut(s) 186, 285, 1072
Alw26I GTCTC 2 cut(s) 9, 193
AlwI GGATC 4 cut(s) 174, 743, 1334, 1393
Ama87I CYCGRG 1 cut(s) 365
Aor13HI TCCGGA 1 cut(s) 610
AoxI GGCC 5 cut(s) 276, 337, 369, 721, 1276
ApeKI GCWGC 6 cut(s) 226, 763, 950, 1129, 1234, 1430
ArsI GACNNNNNNTTYG 2 cut(s) 848, 880
Asp700I GAANNNNTTC 1 cut(s) 296
AspA2I CCTAGG 1 cut(s) 1063
AspLEI GCGC 3 cut(s) 426, 440, 453
AspS9I GGNCC 5 cut(s) 338, 370, 722, 1090, 1276
AsuC2I CCSGG 3 cut(s) 287, 725, 753
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 1 cut(s) 1090
AvrII CCTAGG 1 cut(s) 1063
BanI GGYRCC 2 cut(s) 200, 432
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 3 cut(s) 186, 285, 1072
BbvCI CCTCAGC 1 cut(s) 876
BbvI GCAGC 6 cut(s) 238, 750, 962, 1141, 1246, 1417
BceAI ACGGC 7 cut(s) 214, 251, 310, 362, 433, 470, 1211
BcgI CGANNNNNNTGC 4 cut(s) 376, 410, 1200, 1234
BciT130I CCWGG 1 cut(s) 400
BciVI GTATCC 2 cut(s) 496, 1194
BclI TGATCA 1 cut(s) 924
BcnI CCSGG 3 cut(s) 287, 725, 753
BcoDI GTCTC 2 cut(s) 9, 193
BfaI CTAG 3 cut(s) 960, 1064, 1197
BfuI GTATCC 2 cut(s) 496, 1194
BlnI CCTAGG 1 cut(s) 1063
Bme1390I CCNGG 4 cut(s) 287, 400, 725, 753
Bme18I GGWCC 1 cut(s) 1090
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 5 cut(s) 338, 370, 722, 1090, 1276
BmiI GGNNCC 4 cut(s) 202, 340, 434, 1092
BmrFI CCNGG 4 cut(s) 287, 400, 725, 753
BmsI GCATC 3 cut(s) 1040, 1234, 1242
BpmI CTGGAG 1 cut(s) 808
Bpu10I CCTNAGC 1 cut(s) 876
BpuMI CCSGG 3 cut(s) 287, 725, 753
BsaBI GATNNNNATC 1 cut(s) 1460
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 7 cut(s) 162, 213, 273, 415, 558, 767, 1063
BsaWI WCCGGW 2 cut(s) 118, 610
BsaXI ACNNNNNCTCC 6 cut(s) 80, 110, 188, 218, 1032, 1062
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 1382
Bse118I RCCGGY 3 cut(s) 111, 128, 350
Bse1I ACTGG 3 cut(s) 434, 1142, 1279
Bse3DI GCAATG 1 cut(s) 1050
Bse8I GATNNNNATC 1 cut(s) 1460
BseAI TCCGGA 1 cut(s) 610
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 7 cut(s) 162, 213, 273, 415, 558, 767, 1063
BseGI GGATG 5 cut(s) 568, 688, 733, 799, 1023
BseJI GATNNNNATC 1 cut(s) 1460
BseLI CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 1382
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 4 cut(s) 20, 867, 1085, 1321
BseNI ACTGG 3 cut(s) 434, 1142, 1279
BseXI GCAGC 6 cut(s) 238, 750, 962, 1141, 1246, 1417
BseYI CCCAGC 2 cut(s) 1101, 1375
BsgI GTGCAG 1 cut(s) 313
BshFI GGCC 5 cut(s) 278, 339, 371, 723, 1278
BshNI GGYRCC 2 cut(s) 200, 432
BsiHKAI GWGCWC 3 cut(s) 186, 285, 1072
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
BslFI GGGAC 1 cut(s) 1076
BslI CCNNNNNNNGG 5 cut(s) 111, 118, 285, 367, 1382
BsmAI GTCTC 2 cut(s) 9, 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 1076
BsmI GAATGC 1 cut(s) 1447
BsnI GGCC 5 cut(s) 278, 339, 371, 723, 1278
BsoBI CYCGRG 1 cut(s) 365
Bsp1286I GDGCHC 3 cut(s) 186, 285, 1072
Bsp13I TCCGGA 1 cut(s) 610
Bsp143I GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
Bsp68I TCGCGA 1 cut(s) 491
BspANI GGCC 5 cut(s) 278, 339, 371, 723, 1278
BspCNI CTCAG 4 cut(s) 19, 868, 1084, 1320
BspEI TCCGGA 1 cut(s) 610
BspHI TCATGA 1 cut(s) 1357
BspLI GGNNCC 4 cut(s) 202, 340, 434, 1092
BspPI GGATC 4 cut(s) 174, 743, 1334, 1393
BspT107I GGYRCC 2 cut(s) 200, 432
BsrDI GCAATG 1 cut(s) 1050
BsrFI RCCGGY 3 cut(s) 111, 128, 350
BsrI ACTGG 3 cut(s) 434, 1142, 1279
BssAI RCCGGY 3 cut(s) 111, 128, 350
BssECI CCNNGG 7 cut(s) 162, 213, 273, 415, 558, 767, 1063
BssMI GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
BssNI GRCGYC 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 1063
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 3 cut(s) 759, 957, 1403
Bst6I CTCTTC 2 cut(s) 1011, 1341
BstACI GRCGYC 1 cut(s) 264
BstC8I GCNNGC 5 cut(s) 308, 352, 422, 771, 1239
BstDEI CTNAG 4 cut(s) 6, 876, 1071, 1307
BstDSI CCRYGG 4 cut(s) 162, 213, 415, 767
BstEII GGTNACC 2 cut(s) 121, 1058
BstF5I GGATG 5 cut(s) 568, 688, 733, 799, 1023
BstHHI GCGC 3 cut(s) 426, 440, 453
BstKTI GATC 8 cut(s) 169, 535, 751, 927, 1023, 1179, 1329, 1388
BstMAI GTCTC 2 cut(s) 9, 193
BstMBI GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
BstMWI GCNNNNNNNGC 9 cut(s) 161, 303, 319, 336, 430, 537, 1049, 1138, 1208
BstNI CCWGG 1 cut(s) 400
BstNSI RCATGY 1 cut(s) 412
BstPI GGTNACC 2 cut(s) 121, 1058
BstSCI CCNGG 4 cut(s) 285, 398, 723, 751
BstV1I GCAGC 6 cut(s) 238, 750, 962, 1141, 1246, 1417
BsuI GTATCC 2 cut(s) 496, 1194
BsuRI GGCC 5 cut(s) 278, 339, 371, 723, 1278
BtgI CCRYGG 4 cut(s) 162, 213, 415, 767
BtgZI GCGATG 2 cut(s) 681, 877
BtsCI GGATG 5 cut(s) 568, 688, 733, 799, 1023
BtsIMutI CAGTG 1 cut(s) 589
BtuMI TCGCGA 1 cut(s) 491
Cac8I GCNNGC 5 cut(s) 308, 352, 422, 771, 1239
CciI TCATGA 1 cut(s) 1357
CfoI GCGC 3 cut(s) 426, 440, 453
Cfr10I RCCGGY 3 cut(s) 111, 128, 350
Cfr13I GGNCC 5 cut(s) 338, 370, 722, 1090, 1276
Cfr42I CCGCGG 3 cut(s) 165, 216, 770
CseI GACGC 1 cut(s) 272
Csp6I GTAC 2 cut(s) 218, 744
CviAII CATG 5 cut(s) 388, 409, 526, 823, 1358
CviQI GTAC 2 cut(s) 218, 744
DdeI CTNAG 4 cut(s) 6, 876, 1071, 1307
DpnI GATC 8 cut(s) 168, 534, 750, 926, 1022, 1178, 1328, 1387
DpnII GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
Eam1104I CTCTTC 2 cut(s) 1011, 1341
EarI CTCTTC 2 cut(s) 1011, 1341
EciI GGCGGA 2 cut(s) 148, 328
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 1063
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 1090
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 939, 1224
Eco88I CYCGRG 1 cut(s) 365
Eco91I GGTNACC 2 cut(s) 121, 1058
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 2 cut(s) 370, 1090
EcoO65I GGTNACC 2 cut(s) 121, 1058
EcoRII CCWGG 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 1063
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 1063
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 5 cut(s) 391, 412, 529, 826, 1361
FaqI GGGAC 1 cut(s) 1076
FatI CATG 5 cut(s) 387, 408, 525, 822, 1357
FauI CCCGC 2 cut(s) 349, 762
FbaI TGATCA 1 cut(s) 924
FblI GTMKAC 1 cut(s) 261
FokI GGATG 5 cut(s) 575, 695, 740, 806, 1010
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 3 cut(s) 960, 1064, 1197
GlaI GCGC 3 cut(s) 425, 439, 452
GsaI CCCAGC 2 cut(s) 1105, 1379
GsuI CTGGAG 1 cut(s) 808
HaeIII GGCC 5 cut(s) 278, 339, 371, 723, 1278
HapII CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
HgaI GACGC 1 cut(s) 272
HhaI GCGC 3 cut(s) 426, 440, 453
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 5 cut(s) 391, 412, 529, 826, 1361
Hin6I GCGC 3 cut(s) 424, 438, 451
HinP1I GCGC 3 cut(s) 424, 438, 451
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 5 cut(s) 520, 998, 1169, 1265, 1361
HpaII CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 9 cut(s) 302, 590, 1036, 1074, 1222, 1251, 1310, 1399, 1411
Hpy188III TCNNGA 6 cut(s) 490, 611, 787, 868, 1330, 1358
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 4 cut(s) 266, 383, 398, 491
HpyAV CCTTC 6 cut(s) 23, 52, 103, 151, 503, 617
HpyCH4III ACNGT 3 cut(s) 759, 957, 1403
HpyCH4IV ACGT 2 cut(s) 393, 1354
HpyCH4V TGCA 3 cut(s) 330, 950, 1031
HpyF10VI GCNNNNNNNGC 9 cut(s) 161, 303, 319, 336, 430, 537, 1049, 1138, 1208
HpyF3I CTNAG 4 cut(s) 6, 876, 1071, 1307
HpySE526I ACGT 2 cut(s) 393, 1354
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 5 cut(s) 391, 412, 529, 826, 1361
HspAI GCGC 3 cut(s) 424, 438, 451
Kpn2I TCCGGA 1 cut(s) 610
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 924
KspI CCGCGG 3 cut(s) 165, 216, 770
Kzo9I GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
LmnI GCTCC 3 cut(s) 288, 1040, 1414
Lsp1109I GCAGC 6 cut(s) 238, 750, 962, 1141, 1246, 1417
LweI GCATC 3 cut(s) 1040, 1234, 1242
MaeI CTAG 3 cut(s) 960, 1064, 1197
MaeII ACGT 2 cut(s) 393, 1354
MaeIII GTNAC 4 cut(s) 121, 268, 1058, 1368
MalI GATC 8 cut(s) 168, 534, 750, 926, 1022, 1178, 1328, 1387
MboI GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
MboII GAAGA 4 cut(s) 662, 831, 1028, 1358
MhlI GDGCHC 3 cut(s) 186, 285, 1072
MluCI AATT 2 cut(s) 631, 834
MlyI GAGTC 2 cut(s) 992, 1274
MmeI TCCRAC 1 cut(s) 834
MroI TCCGGA 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 2 cut(s) 38, 936
MslI CAYNNNNRTG 1 cut(s) 626
MspA1I CMGCKG 3 cut(s) 164, 215, 769
MspI CCGG 9 cut(s) 112, 119, 129, 192, 286, 351, 611, 725, 753
MspR9I CCNGG 4 cut(s) 287, 400, 725, 753
Mva1269I GAATGC 1 cut(s) 1447
MvaI CCWGG 1 cut(s) 400
MwoI GCNNNNNNNGC 9 cut(s) 161, 303, 319, 336, 430, 537, 1049, 1138, 1208
NaeI GCCGGC 1 cut(s) 352
NciI CCSGG 3 cut(s) 287, 725, 753
NdeII GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 5 cut(s) 391, 412, 529, 826, 1361
NlaIV GGNNCC 4 cut(s) 202, 340, 434, 1092
NmeAIII GCCGAG 3 cut(s) 304, 516, 583
NmuCI GTSAC 3 cut(s) 121, 268, 1058
NruI TCGCGA 1 cut(s) 491
NspI RCATGY 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 365
PagI TCATGA 1 cut(s) 1357
PciI ACATGT 1 cut(s) 408
PcsI WCGNNNNNNNCGW 4 cut(s) 270, 314, 363, 423
PctI GAATGC 1 cut(s) 1447
PdiI GCCGGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 3 cut(s) 520, 1169, 1361
PflFI GACNNNGTC 1 cut(s) 266
PflMI CCANNNNNTGG 1 cut(s) 1382
PfoI TCCNGGA 2 cut(s) 285, 751
PleI GAGTC 2 cut(s) 992, 1273
PpsI GAGTC 2 cut(s) 992, 1273
PpuMI RGGWCCY 1 cut(s) 1090
PscI ACATGT 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 1090
Psp6I CCWGG 1 cut(s) 398
PspEI GGTNACC 2 cut(s) 121, 1058
PspFI CCCAGC 2 cut(s) 1101, 1375
PspGI CCWGG 1 cut(s) 398
PspN4I GGNNCC 4 cut(s) 202, 340, 434, 1092
PspPI GGNCC 5 cut(s) 338, 370, 722, 1090, 1276
PspPPI RGGWCCY 1 cut(s) 1090
PspXI VCTCGAGB 1 cut(s) 365
PsyI GACNNNGTC 1 cut(s) 266
RruI TCGCGA 1 cut(s) 491
RsaI GTAC 2 cut(s) 219, 745
RsaNI GTAC 2 cut(s) 218, 744
RseI CAYNNNNRTG 1 cut(s) 626
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 3 cut(s) 165, 216, 770
SalI GTCGAC 1 cut(s) 260
SaqAI TTAA 2 cut(s) 38, 936
Sau3AI GATC 8 cut(s) 166, 532, 748, 924, 1020, 1176, 1326, 1385
Sau96I GGNCC 5 cut(s) 338, 370, 722, 1090, 1276
SchI GAGTC 2 cut(s) 992, 1274
ScrFI CCNGG 4 cut(s) 287, 400, 725, 753
SduI GDGCHC 3 cut(s) 186, 285, 1072
SfaNI GCATC 3 cut(s) 1040, 1234, 1242
Sfr274I CTCGAG 1 cut(s) 365
Sfr303I CCGCGG 3 cut(s) 165, 216, 770
SgrBI CCGCGG 3 cut(s) 165, 216, 770
SinI GGWCC 1 cut(s) 1090
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 2 cut(s) 631, 834
SspMI CTAG 3 cut(s) 960, 1064, 1197
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 4 cut(s) 285, 398, 723, 751
StyI CCWWGG 1 cut(s) 1063
TaaI ACNGT 3 cut(s) 759, 957, 1403
TaiI ACGT 2 cut(s) 396, 1357
TaqI TCGA 7 cut(s) 255, 261, 357, 366, 1167, 1179, 1329
TasI AATT 2 cut(s) 631, 834
TatI WGTACW 1 cut(s) 743
TauI GCSGC 4 cut(s) 164, 313, 387, 769
TfiI GAWTC 3 cut(s) 520, 1169, 1361
Tru1I TTAA 2 cut(s) 38, 936
Tru9I TTAA 2 cut(s) 38, 936
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 3 cut(s) 121, 268, 1058
TseI GCWGC 6 cut(s) 226, 763, 950, 1129, 1234, 1430
Tsp45I GTSAC 3 cut(s) 121, 268, 1058
TspDTI ATGAA 3 cut(s) 404, 880, 1374
TspGWI ACGGA 2 cut(s) 169, 538
TspRI CASTG 1 cut(s) 589
Tth111I GACNNNGTC 1 cut(s) 266
Van91I CCANNNNNTGG 1 cut(s) 1382
VpaK11BI GGWCC 1 cut(s) 1090
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 365
XmaJI CCTAGG 1 cut(s) 1063
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 3 cut(s) 960, 1064, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.