AT3G24020

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005053
Physical Location & Seq
Forward (+)
8678678 .. 8679656
979 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G24020.1

Sequence Viewer

Length: 732 bp
ATGATGATCAAGCAATCACCATTCTTGCTTTTAACAACAATCTTGTTCACGGTTGCTGTGTTTGTAGCAGCGCTTGATCCTGCACCTGAAGACCCCATTTTCGAACTCTACATGCACGATTTACTTGGAGGAAGCAGCCCCACCGCGAGGCCCATCACGGGTTTGCTCGGGAACATTTACAACGGTCAAGTTCCATTTGCTAAGCAGATTGGTTTCACTCCTCCTGAGAACGGTATAGCCATACCCAATGCAAACGGCGCACTACCAACGGTTAACGGCATCAATGGTGTACCTCTTGGGACCGGCTTGTCTGGTACAGCATACTCTGGTCAGAACCTAAACGGAATTCAGACTCAGTTGGGTCCTGATGGTCTCAGTCTCGGGTTTGGTACCATTACTGTGATCGACGATATACTCACATCTGGTCCTGACTTGGGTTCACAGCCACTTGGTAAGGCTCAAGGAGTTTATGTTGCAAGTTCTGCAGATGGAAGCACACAGATGATGGCTTTCACAGCTATGCTTGAAGGAGGAGAGTACAACGACAACCTTAACTTCTACGGAATTTACAGGATCGGAAGCGCCATGTCGCATCTATCAGTGACTGGAGGAACAGGTAGGTTCAAGAACGCTTGTGGGTTTGCAGAGGTCAGACCATTGATTCCATCAGGTCAGCATGAAGTGGATGGAGCAGAGTCTTTGCTTAGGATCATTGTCCATCTGAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

243

Amino Acids

25.32

Weight (kDa)

4.77

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 124 - 219 1.4e-23 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
Acc65I GGTACC 1 cut(s) 389
AccB1I GGYRCC 1 cut(s) 389
AccII CGCG 1 cut(s) 146
AciI CCGC 1 cut(s) 144
AclWI GGATC 3 cut(s) 71, 581, 716
AcsI RAATTY 2 cut(s) 345, 564
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 5 cut(s) 291, 316, 391, 539, 728
AfeI AGCGCT 1 cut(s) 72
AfiI CCNNNNNNNGG 4 cut(s) 147, 158, 230, 434
AgsI TTSAA 2 cut(s) 527, 625
AluBI AGCT 1 cut(s) 518
AluI AGCT 1 cut(s) 518
Alw26I GTCTC 2 cut(s) 377, 383
AlwI GGATC 3 cut(s) 71, 581, 716
AlwNI CAGNNNCTG 1 cut(s) 605
Ama87I CYCGRG 2 cut(s) 167, 380
Aor51HI AGCGCT 1 cut(s) 72
AoxI GGCC 1 cut(s) 149
ApeKI GCWGC 2 cut(s) 68, 135
ApoI RAATTY 2 cut(s) 345, 564
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
Asp718I GGTACC 1 cut(s) 389
AspLEI GCGC 3 cut(s) 73, 260, 584
AspS9I GGNCC 4 cut(s) 150, 300, 362, 425
AsuHPI GGTGA 1 cut(s) 9
AsuII TTCGAA 1 cut(s) 102
AvaI CYCGRG 2 cut(s) 167, 380
AvaII GGWCC 3 cut(s) 300, 362, 425
BanI GGYRCC 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 80, 147
BccI CCATC 7 cut(s) 161, 362, 482, 499, 673, 680, 726
BceAI ACGGC 2 cut(s) 271, 292
BclI TGATCA 1 cut(s) 6
BcoDI GTCTC 2 cut(s) 377, 383
BfmI CTRYAG 1 cut(s) 483
BfoI RGCGCY 2 cut(s) 74, 585
BisI GCNGC 2 cut(s) 69, 136
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 2 cut(s) 70, 137
BmcAI AGTACT 1 cut(s) 728
Bme18I GGWCC 3 cut(s) 300, 362, 425
BmeT110I CYCGRG 2 cut(s) 167, 380
BmgT120I GGNCC 4 cut(s) 150, 300, 362, 425
BmiI GGNNCC 3 cut(s) 301, 363, 391
BmsI GCATC 2 cut(s) 288, 601
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 704
Bpu1102I GCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 377
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 158, 230, 434
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 610
BseGI GGATG 1 cut(s) 691
BseLI CCNNNNNNNGG 4 cut(s) 147, 158, 230, 434
BseMII CTCAG 3 cut(s) 216, 368, 388
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 2 cut(s) 210, 546
BseXI GCAGC 2 cut(s) 80, 147
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 1 cut(s) 151
BshNI GGYRCC 1 cut(s) 389
BsiHKCI CYCGRG 2 cut(s) 167, 380
BsiSI CCGG 1 cut(s) 303
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 4 cut(s) 147, 158, 230, 434
BsmAI GTCTC 2 cut(s) 377, 383
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 1 cut(s) 151
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 2 cut(s) 167, 380
Bsp119I TTCGAA 1 cut(s) 102
Bsp143I GATC 5 cut(s) 6, 76, 402, 573, 708
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 1 cut(s) 144
BspANI GGCC 1 cut(s) 151
BspCNI CTCAG 3 cut(s) 217, 367, 387
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 3 cut(s) 301, 363, 391
BspMAI CTGCAG 1 cut(s) 487
BspPI GGATC 3 cut(s) 71, 581, 716
BspT104I TTCGAA 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 1 cut(s) 302
BssMI GATC 5 cut(s) 6, 76, 402, 573, 708
Bst4CI ACNGT 5 cut(s) 52, 185, 233, 271, 400
BstAPI GCANNNNNTGC 1 cut(s) 482
BstBI TTCGAA 1 cut(s) 102
BstDEI CTNAG 5 cut(s) 201, 225, 354, 374, 704
BstF5I GGATG 1 cut(s) 691
BstFNI CGCG 1 cut(s) 146
BstH2I RGCGCY 2 cut(s) 74, 585
BstHHI GCGC 3 cut(s) 73, 260, 584
BstKTI GATC 5 cut(s) 9, 79, 405, 576, 711
BstMAI GTCTC 2 cut(s) 377, 383
BstMBI GATC 5 cut(s) 6, 76, 402, 573, 708
BstMWI GCNNNNNNNGC 3 cut(s) 257, 482, 515
BstNSI RCATGY 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 483
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 80, 147
BstV2I GAAGAC 1 cut(s) 96
BsuRI GGCC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 691
BtsIMutI CAGTG 1 cut(s) 606
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 3 cut(s) 73, 260, 584
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 4 cut(s) 150, 300, 362, 425
Csp6I GTAC 5 cut(s) 290, 315, 390, 538, 727
CviAII CATG 3 cut(s) 112, 586, 677
CviJI RGCY 8 cut(s) 138, 151, 239, 306, 445, 458, 509, 518
CviKI_1 RGCY 8 cut(s) 138, 151, 239, 306, 445, 458, 509, 518
CviQI GTAC 5 cut(s) 290, 315, 390, 538, 727
DdeI CTNAG 5 cut(s) 201, 225, 354, 374, 704
DpnI GATC 5 cut(s) 8, 78, 404, 575, 710
DpnII GATC 5 cut(s) 6, 76, 402, 573, 708
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 3 cut(s) 300, 362, 425
Eco47III AGCGCT 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 108
Eco88I CYCGRG 2 cut(s) 167, 380
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 345
FaeI CATG 3 cut(s) 115, 589, 680
FaiI YATR 9 cut(s) 113, 236, 242, 322, 413, 471, 521, 587, 678
FaqI GGGAC 1 cut(s) 313
FatI CATG 3 cut(s) 111, 585, 676
FbaI TGATCA 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 69, 136
FokI GGATG 1 cut(s) 698
Fsp4HI GCNGC 2 cut(s) 69, 136
GlaI GCGC 3 cut(s) 72, 259, 583
GluI GCNGC 2 cut(s) 69, 136
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 2 cut(s) 74, 585
HaeIII GGCC 1 cut(s) 151
HapII CCGG 1 cut(s) 303
HhaI GCGC 3 cut(s) 73, 260, 584
Hin1II CATG 3 cut(s) 115, 589, 680
Hin6I GCGC 3 cut(s) 71, 258, 582
HinP1I GCGC 3 cut(s) 71, 258, 582
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 3 cut(s) 352, 661, 695
HpaI GTTAAC 1 cut(s) 274
HpaII CCGG 1 cut(s) 303
HphI GGTGA 1 cut(s) 9
Hpy166II GTNNAC 4 cut(s) 48, 274, 290, 440
Hpy188I TCNGA 5 cut(s) 333, 351, 578, 653, 723
Hpy188III TCNNGA 5 cut(s) 169, 224, 365, 428, 625
Hpy8I GTNNAC 4 cut(s) 48, 274, 290, 440
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 1 cut(s) 521
HpyCH4III ACNGT 5 cut(s) 52, 185, 233, 271, 400
HpyCH4V TGCA 6 cut(s) 83, 115, 251, 476, 485, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 482, 515
HpyF3I CTNAG 5 cut(s) 201, 225, 354, 374, 704
Hsp92II CATG 3 cut(s) 115, 589, 680
HspAI GCGC 3 cut(s) 71, 258, 582
KpnI GGTACC 1 cut(s) 393
Ksp22I TGATCA 1 cut(s) 6
KspAI GTTAAC 1 cut(s) 274
Kzo9I GATC 5 cut(s) 6, 76, 402, 573, 708
LmnI GCTCC 1 cut(s) 689
Lsp1109I GCAGC 2 cut(s) 80, 147
LweI GCATC 2 cut(s) 288, 601
MaeIII GTNAC 1 cut(s) 601
MalI GATC 5 cut(s) 8, 78, 404, 575, 710
MboI GATC 5 cut(s) 6, 76, 402, 573, 708
MboII GAAGA 1 cut(s) 101
MluCI AATT 2 cut(s) 345, 564
MlyI GAGTC 2 cut(s) 346, 704
MnlI CCTC 7 cut(s) 122, 141, 231, 303, 524, 602, 640
MseI TTAA 3 cut(s) 32, 273, 552
MslI CAYNNNNRTG 3 cut(s) 398, 500, 518
MspI CCGG 1 cut(s) 303
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 3 cut(s) 257, 482, 515
NdeII GATC 5 cut(s) 6, 76, 402, 573, 708
NlaIII CATG 3 cut(s) 115, 589, 680
NlaIV GGNNCC 3 cut(s) 301, 363, 391
NmuCI GTSAC 1 cut(s) 601
NspI RCATGY 1 cut(s) 115
NspV TTCGAA 1 cut(s) 102
PfeI GAWTC 1 cut(s) 661
PkrI GCNGC 2 cut(s) 70, 137
PleI GAGTC 2 cut(s) 346, 703
PpsI GAGTC 2 cut(s) 346, 703
PpuMI RGGWCCY 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 362
PspN4I GGNNCC 3 cut(s) 301, 363, 391
PspPI GGNCC 4 cut(s) 150, 300, 362, 425
PspPPI RGGWCCY 1 cut(s) 362
PstI CTGCAG 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 605
RsaI GTAC 5 cut(s) 291, 316, 391, 539, 728
RsaNI GTAC 5 cut(s) 290, 315, 390, 538, 727
RseI CAYNNNNRTG 3 cut(s) 398, 500, 518
SaqAI TTAA 3 cut(s) 32, 273, 552
SatI GCNGC 2 cut(s) 69, 136
Sau3AI GATC 5 cut(s) 6, 76, 402, 573, 708
Sau96I GGNCC 4 cut(s) 150, 300, 362, 425
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 2 cut(s) 346, 704
SetI ASST 9 cut(s) 88, 295, 339, 520, 552, 619, 623, 651, 673
SfaNI GCATC 2 cut(s) 288, 601
SfcI CTRYAG 1 cut(s) 483
SfuI TTCGAA 1 cut(s) 102
SinI GGWCC 3 cut(s) 300, 362, 425
SmiMI CAYNNNNRTG 3 cut(s) 398, 500, 518
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 2 cut(s) 345, 564
SsiI CCGC 1 cut(s) 144
TaaI ACNGT 5 cut(s) 52, 185, 233, 271, 400
TaqI TCGA 2 cut(s) 102, 405
TasI AATT 2 cut(s) 345, 564
TatI WGTACW 2 cut(s) 537, 726
TfiI GAWTC 1 cut(s) 661
Tru1I TTAA 3 cut(s) 32, 273, 552
Tru9I TTAA 3 cut(s) 32, 273, 552
TscAI CASTG 1 cut(s) 606
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 2 cut(s) 68, 135
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 1 cut(s) 693
TspGWI ACGGA 2 cut(s) 357, 576
TspRI CASTG 1 cut(s) 606
VpaK11BI GGWCC 3 cut(s) 300, 362, 425
XapI RAATTY 2 cut(s) 345, 564
XceI RCATGY 1 cut(s) 115
ZrmI AGTACT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.