MD13G1229900.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005053
Physical Location & Seq
Reverse (-)
22567679 .. 22568416
738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1229900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGTTCAACCAGTCACTTCTTTCGATTTTGGTCACATTGCTAATTGCCTCAGTGGTCAAGGCAACAGTTGACCTAGTCCAAGCCAGTGGCCATGAGCCAATCCTTGAGATGTACATGCATGACATCTTGGGAGGCAGCAGCCCCACAGCTAGGCCCATCACCGGTCTACTTGGCAACATCTACGGCGGCCAAGTGCCCTTTGCCAGGCCCATAGGCTTCCTGCCACCACAAGGCGGGGTAGCCATTCCCAATGCTAATGGAGCCATTCCAACCGTCAACGGCGTTAATGGGATTCCACTTGGCACCGGCTTAGCTGGTATAACATTCTCTGGAAAACAAAGTGGTCAGGTTCCTCAGACTCCATTAAGTGCAGACGGTTTGAGCCTGGGATTTGGAACCATCACTGTCATCGATGATATTCTGACGACATCACCTGAGATGGGCTCGCAGACAATTGGAAAGGCTCAGGGAGTCTACGTGGCCAGCTCGGCTGACGGGTCGACGCAAATGATGGCGTTCACAGCTATGATTGAAGGAGGTGAGTATGGGGACACCTTGAACTTCTATGGGATATTCAAAATTGGGAGCTCCATGTCACACTTGTCTGTGACTGGAGGGACTGGCAAGTTCAGGAGTGCAAGTGGGTTTGCTGAGGTCAGGTCCCTCATACCTCCTGGCCAACACGTCACTGATGGGGTTGAGACTTTGCTCAGGATCGTCGTCCATCTGAGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

246

Amino Acids

25.15

Weight (kDa)

5.4

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 111 - 221 3.6e-25 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 302
AccI GTMKAC 3 cut(s) 166, 474, 500
AciI CCGC 2 cut(s) 186, 234
AclWI GGATC 1 cut(s) 722
AcoI YGGCCR 4 cut(s) 88, 187, 480, 676
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 6 cut(s) 150, 161, 204, 230, 233, 440
AflIII ACRYGT 1 cut(s) 682
AgeI ACCGGT 1 cut(s) 161
AgsI TTSAA 4 cut(s) 7, 533, 559, 577
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 3 cut(s) 203, 384, 673
AluBI AGCT 5 cut(s) 149, 314, 486, 524, 588
AluI AGCT 5 cut(s) 149, 314, 486, 524, 588
Alw21I GWGCWC 1 cut(s) 590
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 1 cut(s) 722
AoxI GGCC 6 cut(s) 88, 152, 187, 206, 480, 676
ApeKI GCWGC 2 cut(s) 135, 138
AsiGI ACCGGT 1 cut(s) 161
AspS9I GGNCC 3 cut(s) 153, 207, 660
AsuHPI GGTGA 3 cut(s) 151, 423, 551
AvaII GGWCC 1 cut(s) 660
BaeGI GKGCMC 1 cut(s) 198
BalI TGGCCA 3 cut(s) 90, 482, 678
BanI GGYRCC 1 cut(s) 302
BanII GRGCYC 2 cut(s) 446, 590
Bbv12I GWGCWC 1 cut(s) 590
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 2 cut(s) 147, 150
BccI CCATC 6 cut(s) 164, 407, 433, 505, 686, 732
BceAI ACGGC 2 cut(s) 199, 295
BciT130I CCWGG 3 cut(s) 205, 386, 675
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 2 cut(s) 74, 150
BglI GCCNNNNNGGC 1 cut(s) 488
BisI GCNGC 3 cut(s) 136, 139, 187
BlpI GCTNAGC 1 cut(s) 310
BlsI GCNGC 3 cut(s) 137, 140, 188
Bme1390I CCNGG 3 cut(s) 205, 386, 675
Bme18I GGWCC 1 cut(s) 660
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 3 cut(s) 153, 207, 660
BmiI GGNNCC 5 cut(s) 262, 304, 351, 397, 662
BmrFI CCNGG 3 cut(s) 205, 386, 675
BplI GAGNNNNNCTC 2 cut(s) 428, 460
BpmI CTGGAG 1 cut(s) 633
Bpu10I CCTNAGC 3 cut(s) 465, 651, 710
Bpu1102I GCTNAGC 1 cut(s) 310
BpuEI CTTGAG 1 cut(s) 125
Bsa29I ATCGAT 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 478
BsaJI CCNNGG 1 cut(s) 385
BsaWI WCCGGW 1 cut(s) 161
BsaXI ACNNNNNCTCC 4 cut(s) 462, 492, 625, 655
Bsc4I CCNNNNNNNGG 6 cut(s) 150, 161, 204, 230, 233, 440
Bse118I RCCGGY 2 cut(s) 161, 305
Bse1I ACTGG 4 cut(s) 10, 84, 616, 625
Bse3DI GCAATG 1 cut(s) 35
BseBI CCWGG 3 cut(s) 205, 386, 675
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 385
BseLI CCNNNNNNNGG 6 cut(s) 150, 161, 204, 230, 233, 440
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 7 cut(s) 63, 368, 426, 479, 642, 719, 724
BseNI ACTGG 4 cut(s) 10, 84, 616, 625
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 2 cut(s) 147, 150
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 6 cut(s) 90, 154, 189, 208, 482, 678
BshNI GGYRCC 1 cut(s) 302
BshTI ACCGGT 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 590
BsiSI CCGG 2 cut(s) 162, 306
BslFI GGGAC 3 cut(s) 563, 631, 646
BslI CCNNNNNNNGG 6 cut(s) 150, 161, 204, 230, 233, 440
BsmAI GTCTC 1 cut(s) 695
BsmFI GGGAC 3 cut(s) 563, 631, 646
BsnI GGCC 6 cut(s) 90, 154, 189, 208, 482, 678
Bsp1286I GDGCHC 3 cut(s) 198, 446, 590
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 1 cut(s) 714
Bsp1720I GCTNAGC 1 cut(s) 310
BspACI CCGC 2 cut(s) 186, 234
BspANI GGCC 6 cut(s) 90, 154, 189, 208, 482, 678
BspCNI CTCAG 7 cut(s) 62, 367, 427, 478, 643, 720, 723
BspDI ATCGAT 1 cut(s) 411
BspLI GGNNCC 5 cut(s) 262, 304, 351, 397, 662
BspPI GGATC 1 cut(s) 722
BspT107I GGYRCC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 161, 305
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 4 cut(s) 10, 84, 616, 625
BssAI RCCGGY 2 cut(s) 161, 305
BssECI CCNNGG 1 cut(s) 385
BssMI GATC 1 cut(s) 714
Bst2UI CCWGG 3 cut(s) 205, 386, 675
Bst4CI ACNGT 4 cut(s) 67, 274, 377, 406
BstAUI TGTACA 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 2 cut(s) 446, 484
BstDEI CTNAG 8 cut(s) 49, 310, 354, 435, 465, 651, 710, 728
BstENI CCTNNNNNAGG 1 cut(s) 202
BstKTI GATC 1 cut(s) 717
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 1 cut(s) 714
BstMWI GCNNNNNNNGC 3 cut(s) 260, 488, 521
BstNI CCWGG 3 cut(s) 205, 386, 675
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 203, 384, 673
BstSLI GKGCMC 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 147, 150
BstXI CCANNNNNNTGG 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 6 cut(s) 90, 154, 189, 208, 482, 678
BsuTUI ATCGAT 1 cut(s) 411
BtrI CACGTC 1 cut(s) 685
BtsIMutI CAGTG 4 cut(s) 57, 91, 402, 687
Cac8I GCNNGC 2 cut(s) 446, 484
Cfr10I RCCGGY 2 cut(s) 161, 305
Cfr13I GGNCC 3 cut(s) 153, 207, 660
ClaI ATCGAT 1 cut(s) 411
CseI GACGC 1 cut(s) 511
Csp6I GTAC 1 cut(s) 112
CspAI ACCGGT 1 cut(s) 161
CviAII CATG 4 cut(s) 92, 115, 119, 592
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 8 cut(s) 49, 310, 354, 435, 465, 651, 710, 728
DpnI GATC 1 cut(s) 716
DpnII GATC 1 cut(s) 714
EaeI YGGCCR 4 cut(s) 88, 187, 480, 676
Ecl136II GAGCTC 1 cut(s) 588
Eco24I GRGCYC 2 cut(s) 446, 590
Eco47I GGWCC 1 cut(s) 660
Eco53kI GAGCTC 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 588
EcoNI CCTNNNNNAGG 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 660
EcoRII CCWGG 3 cut(s) 203, 384, 673
EcoT22I ATGCAT 1 cut(s) 120
EcoT38I GRGCYC 2 cut(s) 446, 590
FaeI CATG 4 cut(s) 95, 118, 122, 595
FaqI GGGAC 3 cut(s) 563, 631, 646
FatI CATG 4 cut(s) 91, 114, 118, 591
FauI CCCGC 1 cut(s) 227
FblI GTMKAC 3 cut(s) 166, 474, 500
Fnu4HI GCNGC 3 cut(s) 136, 139, 187
FriOI GRGCYC 2 cut(s) 446, 590
Fsp4HI GCNGC 3 cut(s) 136, 139, 187
FspBI CTAG 2 cut(s) 74, 150
GluI GCNGC 3 cut(s) 136, 139, 187
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 6 cut(s) 90, 154, 189, 208, 482, 678
HapII CCGG 2 cut(s) 162, 306
HgaI GACGC 1 cut(s) 511
Hin1II CATG 4 cut(s) 95, 118, 122, 595
HincII GTYRAC 3 cut(s) 70, 277, 501
HindII GTYRAC 3 cut(s) 70, 277, 501
HinfI GANTC 3 cut(s) 292, 358, 471
HpaII CCGG 2 cut(s) 162, 306
HphI GGTGA 3 cut(s) 151, 423, 551
Hpy166II GTNNAC 6 cut(s) 70, 167, 277, 475, 501, 519
Hpy188I TCNGA 3 cut(s) 357, 423, 729
Hpy188III TCNNGA 3 cut(s) 330, 631, 712
Hpy8I GTNNAC 6 cut(s) 70, 167, 277, 475, 501, 519
Hpy99I CGWCG 2 cut(s) 505, 722
HpyAV CCTTC 1 cut(s) 527
HpyCH4III ACNGT 4 cut(s) 67, 274, 377, 406
HpyCH4IV ACGT 2 cut(s) 477, 684
HpyCH4V TGCA 3 cut(s) 118, 371, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 488, 521
HpyF3I CTNAG 8 cut(s) 49, 310, 354, 435, 465, 651, 710, 728
HpySE526I ACGT 2 cut(s) 477, 684
Hsp92II CATG 4 cut(s) 95, 118, 122, 595
Kzo9I GATC 1 cut(s) 714
LmnI GCTCC 3 cut(s) 260, 585, 593
Lsp1109I GCAGC 2 cut(s) 147, 150
MaeI CTAG 2 cut(s) 74, 150
MaeII ACGT 2 cut(s) 477, 684
MaeIII GTNAC 6 cut(s) 12, 31, 594, 607, 685, 731
MalI GATC 1 cut(s) 716
MboI GATC 1 cut(s) 714
MfeI CAATTG 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 198, 446, 590
MlsI TGGCCA 3 cut(s) 90, 482, 678
MluCI AATT 3 cut(s) 42, 453, 579
MluNI TGGCCA 3 cut(s) 90, 482, 678
MlyI GAGTC 2 cut(s) 352, 480
MmeI TCCRAC 1 cut(s) 293
MnlI CCTC 8 cut(s) 58, 125, 363, 530, 608, 646, 674, 681
Mox20I TGGCCA 3 cut(s) 90, 482, 678
Mph1103I ATGCAT 1 cut(s) 120
MscI TGGCCA 3 cut(s) 90, 482, 678
MseI TTAA 2 cut(s) 285, 365
MslI CAYNNNNRTG 1 cut(s) 524
Msp20I TGGCCA 3 cut(s) 90, 482, 678
MspI CCGG 2 cut(s) 162, 306
MspR9I CCNGG 3 cut(s) 205, 386, 675
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 3 cut(s) 205, 386, 675
MwoI GCNNNNNNNGC 3 cut(s) 260, 488, 521
NdeII GATC 1 cut(s) 714
NlaIII CATG 4 cut(s) 95, 118, 122, 595
NlaIV GGNNCC 5 cut(s) 262, 304, 351, 397, 662
NmeAIII GCCGAG 1 cut(s) 467
NmuCI GTSAC 5 cut(s) 12, 31, 594, 607, 685
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 1 cut(s) 118
PfeI GAWTC 1 cut(s) 292
PflFI GACNNNGTC 1 cut(s) 74
PinAI ACCGGT 1 cut(s) 161
PkrI GCNGC 3 cut(s) 137, 140, 188
PleI GAGTC 2 cut(s) 352, 479
PpsI GAGTC 2 cut(s) 352, 479
Ppu21I YACGTR 1 cut(s) 478
PpuMI RGGWCCY 1 cut(s) 660
Psp124BI GAGCTC 1 cut(s) 590
Psp5II RGGWCCY 1 cut(s) 660
Psp6I CCWGG 3 cut(s) 203, 384, 673
PspGI CCWGG 3 cut(s) 203, 384, 673
PspN4I GGNNCC 5 cut(s) 262, 304, 351, 397, 662
PspPI GGNCC 3 cut(s) 153, 207, 660
PspPPI RGGWCCY 1 cut(s) 660
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 524
SacI GAGCTC 1 cut(s) 590
SalI GTCGAC 1 cut(s) 499
SaqAI TTAA 2 cut(s) 285, 365
SatI GCNGC 3 cut(s) 136, 139, 187
Sau3AI GATC 1 cut(s) 714
Sau96I GGNCC 3 cut(s) 153, 207, 660
SchI GAGTC 2 cut(s) 352, 480
ScrFI CCNGG 3 cut(s) 205, 386, 675
SduI GDGCHC 3 cut(s) 198, 446, 590
SinI GGWCC 1 cut(s) 660
SmiMI CAYNNNNRTG 1 cut(s) 524
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 3 cut(s) 42, 453, 579
SsiI CCGC 2 cut(s) 186, 234
SspMI CTAG 2 cut(s) 74, 150
SstI GAGCTC 1 cut(s) 590
StyD4I CCNGG 3 cut(s) 203, 384, 673
TaaI ACNGT 4 cut(s) 67, 274, 377, 406
TaiI ACGT 2 cut(s) 480, 687
TaqI TCGA 3 cut(s) 23, 411, 500
TasI AATT 3 cut(s) 42, 453, 579
TatI WGTACW 1 cut(s) 111
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 2 cut(s) 285, 365
Tru9I TTAA 2 cut(s) 285, 365
TscAI CASTG 4 cut(s) 57, 91, 409, 694
TseFI GTSAC 5 cut(s) 12, 31, 594, 607, 685
TseI GCWGC 2 cut(s) 135, 138
Tsp45I GTSAC 5 cut(s) 12, 31, 594, 607, 685
TspRI CASTG 4 cut(s) 57, 91, 409, 694
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 660
XagI CCTNNNNNAGG 1 cut(s) 202
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 3 cut(s) 166, 474, 500
XspI CTAG 2 cut(s) 74, 150
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.