Rh4DG065800

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005053
Physical Location & Seq
Forward (+)
9894928 .. 9896207
1280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG065800.1

Sequence Viewer

Length: 756 bp
ATGATGGTCAAGCAATTATCCTCAGTCTATTCCATTTTGCTCTCTATTCTAGTTGCCTCAGTCCATGTACTATTAGTCAATGCAGCAGCTGACCCAGTCAGGGCCACTGAACATGAACCAATTCTTGAGATGTACATGCATGACATTCTTGGGGGCAGCAGCCCAACAGCTAGGCCTATAACTGGCCTGCTAGGCAACATCTATGGAGGTCAAGTTCCCTTTGCAAAGCCAATAGGGTTCCTTCCACCACAAGGTGGTGTCCCCATCCCCAATGCTAATGGTGCCCTCCCTACTGTCAATGGCATCAATGGGATTCCTCTTGGAACCGGCTTGTCCGGCACAACGTTTTCGGGAAAGCCCATTGGGCAGGCCCAGACCCAGTTGGGCCCTGATGGTCTGAGCCTGGGCTTCGGCACCATCACCGTCATTGATGACATTCTCACCACAGCACCTGAGCTAGGGTCACAGAGTATTGGTAAAGCACAAGGGGTTTATGTGGCAAGTTCAGCAGATGGGTCAAGACAGATGATGGCATTCACTGCTATGATTGAAGGAGGTGAGTATGGTGATAGTCTCAACTTCTATGGTGTGTTTAAGATTGGAAGCCCAATGTCACATTTGTCTGTGACTGGAGGGACTGGGAAGTTCAAGAGTGCTAGTGGTTTTGCTGAGGTGAGATCACTAATCCCTCCTGGTCAACATATTACTGATGGTGCAGAGACTCTGCTGAGGATCATCGTACATCTAAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

251

Amino Acids

25.83

Weight (kDa)

5.81

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 132 - 227 9e-24 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 281, 413
AccB7I CCANNNNNTGG 1 cut(s) 254
AclI AACGTT 1 cut(s) 344
AclWI GGATC 1 cut(s) 740
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 3 cut(s) 69, 134, 741
AfiI CCNNNNNNNGG 5 cut(s) 100, 182, 251, 254, 458
AgsI TTSAA 2 cut(s) 551, 649
AjnI CCWGG 2 cut(s) 402, 691
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
AluBI AGCT 4 cut(s) 89, 170, 457, 750
AluI AGCT 4 cut(s) 89, 170, 457, 750
Alw26I GTCTC 2 cut(s) 578, 713
AlwI GGATC 1 cut(s) 740
AlwNI CAGNNNCTG 2 cut(s) 89, 452
AoxI GGCC 5 cut(s) 102, 173, 184, 369, 385
ApaI GGGCCC 1 cut(s) 389
ApeKI GCWGC 4 cut(s) 83, 86, 156, 159
Asp700I GAANNNNTTC 1 cut(s) 120
AspS9I GGNCC 4 cut(s) 102, 370, 385, 386
AsuHPI GGTGA 5 cut(s) 412, 433, 569, 578, 685
BaeGI GKGCMC 2 cut(s) 286, 389
BanI GGYRCC 2 cut(s) 281, 413
BanII GRGCYC 1 cut(s) 389
BbvCI CCTCAGC 2 cut(s) 669, 728
BbvI GCAGC 4 cut(s) 95, 98, 168, 171
BccI CCATC 6 cut(s) 272, 386, 425, 506, 523, 704
BciT130I CCWGG 2 cut(s) 404, 693
BcoDI GTCTC 2 cut(s) 578, 713
BfaI CTAG 5 cut(s) 50, 171, 191, 458, 657
BglI GCCNNNNNGGC 2 cut(s) 192, 364
BisI GCNGC 4 cut(s) 84, 87, 157, 160
BlsI GCNGC 4 cut(s) 85, 88, 158, 161
Bme1390I CCNGG 2 cut(s) 404, 693
BmgT120I GGNCC 4 cut(s) 102, 370, 385, 386
BmiI GGNNCC 5 cut(s) 239, 283, 325, 387, 415
BmrFI CCNGG 2 cut(s) 404, 693
BmrI ACTGGG 3 cut(s) 89, 373, 648
BmsI GCATC 1 cut(s) 312
BmuI ACTGGG 3 cut(s) 89, 373, 648
BpmI CTGGAG 1 cut(s) 651
Bpu10I CCTNAGC 3 cut(s) 453, 669, 728
BpuEI CTTGAG 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 182, 251, 254, 458
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 5 cut(s) 95, 187, 379, 634, 643
BseBI CCWGG 2 cut(s) 404, 693
BseDI CCNNGG 1 cut(s) 403
BseGI GGATG 1 cut(s) 264
BseLI CCNNNNNNNGG 5 cut(s) 100, 182, 251, 254, 458
BseMII CTCAG 6 cut(s) 36, 72, 389, 444, 660, 719
BseNI ACTGG 5 cut(s) 95, 187, 379, 634, 643
BseSI GKGCMC 2 cut(s) 286, 389
BseXI GCAGC 4 cut(s) 95, 98, 168, 171
BsgI GTGCAG 1 cut(s) 735
BshFI GGCC 5 cut(s) 104, 175, 186, 371, 387
BshNI GGYRCC 2 cut(s) 281, 413
BsiSI CCGG 2 cut(s) 327, 336
BslFI GGGAC 2 cut(s) 245, 649
BslI CCNNNNNNNGG 5 cut(s) 100, 182, 251, 254, 458
BsmAI GTCTC 2 cut(s) 578, 713
BsmFI GGGAC 2 cut(s) 245, 649
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 5 cut(s) 104, 175, 186, 371, 387
Bsp120I GGGCCC 1 cut(s) 385
Bsp1286I GDGCHC 2 cut(s) 286, 389
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 2 cut(s) 677, 732
BspANI GGCC 5 cut(s) 104, 175, 186, 371, 387
BspCNI CTCAG 6 cut(s) 35, 71, 390, 445, 661, 720
BspLI GGNNCC 5 cut(s) 239, 283, 325, 387, 415
BspPI GGATC 1 cut(s) 740
BspT107I GGYRCC 2 cut(s) 281, 413
BsrFI RCCGGY 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 132
BsrI ACTGG 5 cut(s) 95, 187, 379, 634, 643
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 2 cut(s) 677, 732
Bst2UI CCWGG 2 cut(s) 404, 693
Bst4CI ACNGT 2 cut(s) 295, 424
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 188, 369
BstDEI CTNAG 7 cut(s) 22, 58, 398, 453, 669, 728, 746
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 264
BstKTI GATC 2 cut(s) 680, 735
BstMAI GTCTC 2 cut(s) 578, 713
BstMBI GATC 2 cut(s) 677, 732
BstMWI GCNNNNNNNGC 6 cut(s) 192, 281, 336, 364, 506, 539
BstNI CCWGG 2 cut(s) 404, 693
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 402, 691
BstSLI GKGCMC 2 cut(s) 286, 389
BstV1I GCAGC 4 cut(s) 95, 98, 168, 171
BsuRI GGCC 5 cut(s) 104, 175, 186, 371, 387
BtsCI GGATG 1 cut(s) 264
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 2 cut(s) 105, 537
Cac8I GCNNGC 2 cut(s) 188, 369
CaiI CAGNNNCTG 2 cut(s) 89, 452
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 4 cut(s) 102, 370, 385, 386
Csp6I GTAC 3 cut(s) 68, 133, 740
CviAII CATG 4 cut(s) 65, 113, 136, 140
CviQI GTAC 3 cut(s) 68, 133, 740
DdeI CTNAG 7 cut(s) 22, 58, 398, 453, 669, 728, 746
DpnI GATC 2 cut(s) 679, 734
DpnII GATC 2 cut(s) 677, 732
DraIII CACNNNGTG 1 cut(s) 254
Eco147I AGGCCT 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 402, 691
EcoT22I ATGCAT 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 389
FaeI CATG 4 cut(s) 68, 116, 139, 143
FaqI GGGAC 2 cut(s) 245, 649
FatI CATG 4 cut(s) 64, 112, 135, 139
Fnu4HI GCNGC 4 cut(s) 84, 87, 157, 160
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 389
Fsp4HI GCNGC 4 cut(s) 84, 87, 157, 160
FspBI CTAG 5 cut(s) 50, 171, 191, 458, 657
GluI GCNGC 4 cut(s) 84, 87, 157, 160
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 5 cut(s) 104, 175, 186, 371, 387
HapII CCGG 2 cut(s) 327, 336
Hin1II CATG 4 cut(s) 68, 116, 139, 143
HincII GTYRAC 1 cut(s) 698
HindII GTYRAC 1 cut(s) 698
HinfI GANTC 2 cut(s) 313, 721
HpaII CCGG 2 cut(s) 327, 336
HphI GGTGA 5 cut(s) 412, 433, 569, 578, 685
Hpy166II GTNNAC 1 cut(s) 698
Hpy188I TCNGA 1 cut(s) 399
Hpy188III TCNNGA 4 cut(s) 125, 351, 519, 649
Hpy8I GTNNAC 1 cut(s) 698
HpyAV CCTTC 2 cut(s) 251, 545
HpyCH4III ACNGT 2 cut(s) 295, 424
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 4 cut(s) 83, 139, 224, 716
HpyF10VI GCNNNNNNNGC 6 cut(s) 192, 281, 336, 364, 506, 539
HpyF3I CTNAG 7 cut(s) 22, 58, 398, 453, 669, 728, 746
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 4 cut(s) 68, 116, 139, 143
Kzo9I GATC 2 cut(s) 677, 732
Lsp1109I GCAGC 4 cut(s) 95, 98, 168, 171
LweI GCATC 1 cut(s) 312
MaeI CTAG 5 cut(s) 50, 171, 191, 458, 657
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 3 cut(s) 462, 612, 625
MalI GATC 2 cut(s) 679, 734
MboI GATC 2 cut(s) 677, 732
MhlI GDGCHC 2 cut(s) 286, 389
MluCI AATT 2 cut(s) 14, 120
MlyI GAGTC 1 cut(s) 715
Mph1103I ATGCAT 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 1 cut(s) 594
MslI CAYNNNNRTG 1 cut(s) 542
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 327, 336
MspR9I CCNGG 2 cut(s) 404, 693
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 2 cut(s) 404, 693
MwoI GCNNNNNNNGC 6 cut(s) 192, 281, 336, 364, 506, 539
NdeII GATC 2 cut(s) 677, 732
NlaIII CATG 4 cut(s) 68, 116, 139, 143
NlaIV GGNNCC 5 cut(s) 239, 283, 325, 387, 415
NmuCI GTSAC 3 cut(s) 462, 612, 625
NsiI ATGCAT 1 cut(s) 141
NspI RCATGY 1 cut(s) 139
PceI AGGCCT 1 cut(s) 175
PctI GAATGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 1 cut(s) 313
PflFI GACNNNGTC 1 cut(s) 95
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 4 cut(s) 85, 88, 158, 161
PleI GAGTC 1 cut(s) 715
PpsI GAGTC 1 cut(s) 715
Psp1406I AACGTT 1 cut(s) 344
Psp6I CCWGG 2 cut(s) 402, 691
PspGI CCWGG 2 cut(s) 402, 691
PspN4I GGNNCC 5 cut(s) 239, 283, 325, 387, 415
PspOMI GGGCCC 1 cut(s) 385
PspPI GGNCC 4 cut(s) 102, 370, 385, 386
PstNI CAGNNNCTG 2 cut(s) 89, 452
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 69, 134, 741
RsaNI GTAC 3 cut(s) 68, 133, 740
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 4 cut(s) 84, 87, 157, 160
Sau3AI GATC 2 cut(s) 677, 732
Sau96I GGNCC 4 cut(s) 102, 370, 385, 386
SchI GAGTC 1 cut(s) 715
ScrFI CCNGG 2 cut(s) 404, 693
SduI GDGCHC 2 cut(s) 286, 389
SfaNI GCATC 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 542
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 2 cut(s) 14, 120
SseBI AGGCCT 1 cut(s) 175
SspMI CTAG 5 cut(s) 50, 171, 191, 458, 657
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 2 cut(s) 402, 691
TaaI ACNGT 2 cut(s) 295, 424
TaiI ACGT 1 cut(s) 347
TasI AATT 2 cut(s) 14, 120
TatI WGTACW 2 cut(s) 67, 132
TfiI GAWTC 1 cut(s) 313
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 2 cut(s) 112, 544
TseFI GTSAC 3 cut(s) 462, 612, 625
TseI GCWGC 4 cut(s) 83, 86, 156, 159
Tsp45I GTSAC 3 cut(s) 462, 612, 625
TspDTI ATGAA 1 cut(s) 129
TspRI CASTG 2 cut(s) 112, 544
Tth111I GACNNNGTC 1 cut(s) 95
Van91I CCANNNNNTGG 1 cut(s) 254
XagI CCTNNNNNAGG 1 cut(s) 456
XceI RCATGY 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 5 cut(s) 50, 171, 191, 458, 657
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.