AT4G13580

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005053
Physical Location & Seq
Forward (+)
7900374 .. 7901328
955 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13580.1

Sequence Viewer

Length: 735 bp
ATGATGAAACAATCACCATTCTCACTTTTAACATCAATCTTTCTCATAGCTGCTCTTTTTACTGCAACTACCGCACTCGATCCTGCACCCGAAGACCCGATTTTTGAATTGTACATGCACGATATACTTGGGGGAAGCAGTCCCACAGCAAGACCCATTACGGGTTTGCTTGGAAACATCTACAACGGTCAAGTACCGTTTGCTAAGCAGATCGGTTTTGTTCCACCGCAAAACGGTGTAGCCATACCCAACGCAAATGGGGCAATGCCAACGGTTAACGGTATCAACGGTATTCCATTAGGTACTGGTTTGTCTGGAACAGCGTTTTCTGGTCAGAATCTAAATGGGATTCAGACTCAGTTGGGTCCTGATGGTCTGAGTCTTGGGTTTGGAACAATCACGGTGATTGACGACATAATCACATCTGGTCCTGACTTGGGGTCACAACCACTTGGTAAGGCTCAAGGTGTTTATGTTGCCAGCTCAGCAGATGGAAGCACGCAGATGATGGCTTTCACAGCTATGCTTGAAGGTGGAGAGTACAATGATAATCTAAATTTCTATGGAATCTATAGGATAGGAAGCGCCATGTCACATCTGTCAGTGACTGGAGGAACCGGAAGGTTTAAGAACGCTTGCGGATTTGCAGAGGTTAGGCCGTTGATTCCTGCGGGCCAACACTTTGTTGATGGAGCTGAGATGCTGTTGAGGATCATTGTCCATCTGAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

244

Amino Acids

25.41

Weight (kDa)

4.99

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 126 - 220 4e-23 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 72, 227, 639, 671
AclWI GGATC 2 cut(s) 74, 719
AcsI RAATTY 1 cut(s) 556
AfaI GTAC 4 cut(s) 113, 195, 304, 542
AfiI CCNNNNNNNGG 3 cut(s) 161, 233, 437
AgsI TTSAA 2 cut(s) 107, 530
AhdI GACNNNNNGTC 1 cut(s) 439
AluBI AGCT 4 cut(s) 50, 483, 521, 695
AluI AGCT 4 cut(s) 50, 483, 521, 695
AlwI GGATC 2 cut(s) 74, 719
AlwNI CAGNNNCTG 1 cut(s) 608
AoxI GGCC 2 cut(s) 656, 673
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 556
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AspLEI GCGC 1 cut(s) 587
AspS9I GGNCC 3 cut(s) 365, 428, 673
AsuHPI GGTGA 2 cut(s) 6, 415
AvaII GGWCC 2 cut(s) 365, 428
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 37
BccI CCATC 5 cut(s) 365, 485, 502, 683, 729
BceAI ACGGC 1 cut(s) 643
BfmI CTRYAG 1 cut(s) 571
BfoI RGCGCY 1 cut(s) 588
BisI GCNGC 1 cut(s) 51
BlpI GCTNAGC 2 cut(s) 204, 484
BlsI GCNGC 1 cut(s) 52
Bme18I GGWCC 2 cut(s) 365, 428
BmeRI GACNNNNNGTC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 365, 428, 673
BmiI GGNNCC 2 cut(s) 366, 616
BmsI GCATC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 630
Bpu1102I GCTNAGC 2 cut(s) 204, 484
BpuEI CTTGAG 1 cut(s) 447
BsaWI WCCGGW 1 cut(s) 617
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 233, 437
Bse1I ACTGG 2 cut(s) 310, 613
Bse3DI GCAATG 1 cut(s) 270
BseLI CCNNNNNNNGG 3 cut(s) 161, 233, 437
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 4 cut(s) 368, 371, 498, 687
BseNI ACTGG 2 cut(s) 310, 613
BseXI GCAGC 1 cut(s) 37
BsgI GTGCAG 1 cut(s) 69
BshFI GGCC 2 cut(s) 658, 675
BsiSI CCGG 1 cut(s) 618
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 3 cut(s) 161, 233, 437
BsmFI GGGAC 1 cut(s) 126
BsnI GGCC 2 cut(s) 658, 675
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 3 cut(s) 79, 210, 711
Bsp1720I GCTNAGC 2 cut(s) 204, 484
BspACI CCGC 4 cut(s) 72, 227, 639, 671
BspANI GGCC 2 cut(s) 658, 675
BspCNI CTCAG 4 cut(s) 369, 370, 497, 688
BspLI GGNNCC 2 cut(s) 366, 616
BspPI GGATC 2 cut(s) 74, 719
BsrDI GCAATG 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 2 cut(s) 310, 613
BssMI GATC 3 cut(s) 79, 210, 711
Bst4CI ACNGT 7 cut(s) 188, 198, 236, 274, 281, 290, 403
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 4 cut(s) 481, 500, 637, 673
BstDEI CTNAG 5 cut(s) 204, 357, 377, 484, 696
BstH2I RGCGCY 1 cut(s) 588
BstHHI GCGC 1 cut(s) 587
BstKTI GATC 3 cut(s) 82, 213, 714
BstMBI GATC 3 cut(s) 79, 210, 711
BstMWI GCNNNNNNNGC 4 cut(s) 71, 260, 485, 518
BstNSI RCATGY 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 571
BstV1I GCAGC 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 99
BsuRI GGCC 2 cut(s) 658, 675
BtsIMutI CAGTG 1 cut(s) 609
Cac8I GCNNGC 4 cut(s) 481, 500, 637, 673
CaiI CAGNNNCTG 1 cut(s) 608
CfoI GCGC 1 cut(s) 587
Cfr13I GGNCC 3 cut(s) 365, 428, 673
Csp6I GTAC 4 cut(s) 112, 194, 303, 541
CviAII CATG 2 cut(s) 115, 589
CviJI RGCY 9 cut(s) 50, 242, 461, 483, 512, 521, 658, 675, 695
CviKI_1 RGCY 9 cut(s) 50, 242, 461, 483, 512, 521, 658, 675, 695
CviQI GTAC 4 cut(s) 112, 194, 303, 541
DdeI CTNAG 5 cut(s) 204, 357, 377, 484, 696
DpnI GATC 3 cut(s) 81, 212, 713
DpnII GATC 3 cut(s) 79, 210, 711
DriI GACNNNNNGTC 1 cut(s) 439
Eam1105I GACNNNNNGTC 1 cut(s) 439
Eco47I GGWCC 2 cut(s) 365, 428
EcoO109I RGGNCCY 1 cut(s) 365
FaeI CATG 2 cut(s) 118, 592
FaqI GGGAC 1 cut(s) 126
FatI CATG 2 cut(s) 114, 588
FauI CCCGC 1 cut(s) 664
Fnu4HI GCNGC 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 51
GlaI GCGC 1 cut(s) 586
GluI GCNGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 588
HaeIII GGCC 2 cut(s) 658, 675
HapII CCGG 1 cut(s) 618
HhaI GCGC 1 cut(s) 587
Hin1II CATG 2 cut(s) 118, 592
Hin6I GCGC 1 cut(s) 585
HinP1I GCGC 1 cut(s) 585
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 6 cut(s) 337, 349, 355, 379, 567, 664
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 618
HphI GGTGA 2 cut(s) 6, 415
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 4 cut(s) 336, 354, 378, 726
Hpy188III TCNNGA 3 cut(s) 315, 368, 431
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 524, 615
HpyCH4III ACNGT 7 cut(s) 188, 198, 236, 274, 281, 290, 403
HpyCH4V TGCA 4 cut(s) 65, 86, 118, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 260, 485, 518
HpyF3I CTNAG 5 cut(s) 204, 357, 377, 484, 696
Hsp92II CATG 2 cut(s) 118, 592
HspAI GCGC 1 cut(s) 585
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 79, 210, 711
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 1 cut(s) 690
MaeIII GTNAC 3 cut(s) 441, 591, 604
MalI GATC 3 cut(s) 81, 212, 713
MboI GATC 3 cut(s) 79, 210, 711
MboII GAAGA 1 cut(s) 104
MluCI AATT 2 cut(s) 107, 556
MlyI GAGTC 2 cut(s) 349, 388
MnlI CCTC 3 cut(s) 605, 643, 702
MseI TTAA 3 cut(s) 29, 276, 627
MslI CAYNNNNRTG 2 cut(s) 503, 521
MspI CCGG 1 cut(s) 618
MwoI GCNNNNNNNGC 4 cut(s) 71, 260, 485, 518
NdeII GATC 3 cut(s) 79, 210, 711
NlaIII CATG 2 cut(s) 118, 592
NlaIV GGNNCC 2 cut(s) 366, 616
NmuCI GTSAC 3 cut(s) 441, 591, 604
NspI RCATGY 1 cut(s) 118
PfeI GAWTC 4 cut(s) 337, 349, 567, 664
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 2 cut(s) 349, 387
PpsI GAGTC 2 cut(s) 349, 387
PpuMI RGGWCCY 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 365
PspN4I GGNNCC 2 cut(s) 366, 616
PspPI GGNCC 3 cut(s) 365, 428, 673
PspPPI RGGWCCY 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 608
RsaI GTAC 4 cut(s) 113, 195, 304, 542
RsaNI GTAC 4 cut(s) 112, 194, 303, 541
RseI CAYNNNNRTG 2 cut(s) 503, 521
SaqAI TTAA 3 cut(s) 29, 276, 627
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 3 cut(s) 79, 210, 711
Sau96I GGNCC 3 cut(s) 365, 428, 673
SchI GAGTC 2 cut(s) 349, 388
SetI ASST 9 cut(s) 52, 304, 469, 485, 523, 535, 626, 654, 697
SfaNI GCATC 1 cut(s) 690
SfcI CTRYAG 1 cut(s) 571
SinI GGWCC 2 cut(s) 365, 428
SmiMI CAYNNNNRTG 2 cut(s) 503, 521
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse9I AATT 2 cut(s) 107, 556
SsiI CCGC 4 cut(s) 72, 227, 639, 671
TaaI ACNGT 7 cut(s) 188, 198, 236, 274, 281, 290, 403
TaqI TCGA 1 cut(s) 78
TasI AATT 2 cut(s) 107, 556
TatI WGTACW 2 cut(s) 111, 540
TfiI GAWTC 4 cut(s) 337, 349, 567, 664
Tru1I TTAA 3 cut(s) 29, 276, 627
Tru9I TTAA 3 cut(s) 29, 276, 627
TscAI CASTG 1 cut(s) 609
TseFI GTSAC 3 cut(s) 441, 591, 604
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 3 cut(s) 441, 591, 604
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 1 cut(s) 609
VpaK11BI GGWCC 2 cut(s) 365, 428
XapI RAATTY 1 cut(s) 556
XceI RCATGY 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.