Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G24020 AT4G13580 |
| fragaria_vesca | FvH4_4g05450 FvH4_4g05460 |
| malus_domestica | MD13G1229800.v1.1 MD13G1229900.v1.1 MD13G1230100.v1.1 |
| prunus_persica | Prupe.1G056700_v2.0.a1 Prupe.1G056800_v2.0.a1 |
| pyrus_communis | pycom13g20250 |
| rosa_chinensis | RchiOBHm_Chr4g0396611 RchiOBHm_Chr4g0396621 |
| rosa_laevigata | RLG00000009515 RLG00000009516 |
| rosa_multiflora | Rmu_co8415101.1_g000001 Rmu_sc0006586.1_g000021 Rmu_sc0006586.1_g000023 Rmu_sc0010969.1_g000003 Rmu_sc0010969.1_g000005 |
| rosa_roxburghii | Rroxscaffold_5G00341470 Rroxscaffold_5G00341490 |
| rosa_rugosa | Rorug03G0348300 Rorug03G0348400 |
| rosa_samantha | Rh4BG068700 Rh4BG068900 Rh4CG076500 Rh4CG076700 Rh4DG065800 Rh4DG065900 |
| rosa_wichuraiana | Rw4G005650 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 275 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 176, 248 |
| AclWI | GGATC | 1 cut(s) | 734 |
| AcoI | YGGCCR | 2 cut(s) | 255, 358 |
| AcsI | RAATTY | 1 cut(s) | 638 |
| AdeI | CACNNNGTG | 1 cut(s) | 248 |
| AfaI | GTAC | 2 cut(s) | 63, 128 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 176, 219, 245, 248, 452 |
| AflIII | ACRYGT | 1 cut(s) | 694 |
| AgsI | TTSAA | 4 cut(s) | 7, 545, 589, 643 |
| AjnI | CCWGG | 3 cut(s) | 360, 396, 685 |
| AluBI | AGCT | 4 cut(s) | 164, 329, 503, 744 |
| AluI | AGCT | 4 cut(s) | 164, 329, 503, 744 |
| Alw26I | GTCTC | 1 cut(s) | 707 |
| AlwI | GGATC | 1 cut(s) | 734 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 446 |
| AoxI | GGCC | 9 cut(s) | 167, 178, 221, 255, 295, 358, 363, 379, 394 |
| ApaI | GGGCCC | 1 cut(s) | 383 |
| ApeKI | GCWGC | 3 cut(s) | 77, 150, 153 |
| ApoI | RAATTY | 1 cut(s) | 638 |
| AspS9I | GGNCC | 5 cut(s) | 364, 379, 380, 394, 510 |
| AsuHPI | GGTGA | 3 cut(s) | 6, 563, 572 |
| AvaII | GGWCC | 1 cut(s) | 510 |
| BaeGI | GKGCMC | 3 cut(s) | 213, 280, 383 |
| BalI | TGGCCA | 2 cut(s) | 257, 360 |
| BanI | GGYRCC | 1 cut(s) | 275 |
| BanII | GRGCYC | 2 cut(s) | 383, 458 |
| BbvCI | CCTCAGC | 2 cut(s) | 663, 722 |
| BbvI | GCAGC | 3 cut(s) | 89, 162, 165 |
| BccI | CCATC | 5 cut(s) | 266, 380, 500, 517, 698 |
| BciT130I | CCWGG | 3 cut(s) | 362, 398, 687 |
| BcoDI | GTCTC | 1 cut(s) | 707 |
| BfaI | CTAG | 1 cut(s) | 165 |
| BfmI | CTRYAG | 1 cut(s) | 159 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 186, 303 |
| BisI | GCNGC | 3 cut(s) | 78, 151, 154 |
| BlpI | GCTNAGC | 1 cut(s) | 325 |
| BlsI | GCNGC | 3 cut(s) | 79, 152, 155 |
| Bme1390I | CCNGG | 3 cut(s) | 362, 398, 687 |
| Bme18I | GGWCC | 1 cut(s) | 510 |
| BmgT120I | GGNCC | 5 cut(s) | 364, 379, 380, 394, 510 |
| BmiI | GGNNCC | 5 cut(s) | 233, 277, 381, 409, 511 |
| BmrFI | CCNGG | 3 cut(s) | 362, 398, 687 |
| BmrI | ACTGGG | 2 cut(s) | 83, 367 |
| BmsI | GCATC | 1 cut(s) | 745 |
| BmuI | ACTGGG | 2 cut(s) | 83, 367 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 440, 472 |
| Bpu10I | CCTNAGC | 2 cut(s) | 663, 722 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 325 |
| BpuEI | CTTGAG | 2 cut(s) | 140, 462 |
| BsaJI | CCNNGG | 1 cut(s) | 397 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 192, 222, 474, 504 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 176, 219, 245, 248, 452 |
| Bse1I | ACTGG | 6 cut(s) | 89, 99, 181, 325, 373, 637 |
| Bse3DI | GCAATG | 1 cut(s) | 716 |
| BseBI | CCWGG | 3 cut(s) | 362, 398, 687 |
| BseDI | CCNNGG | 1 cut(s) | 397 |
| BseGI | GGATG | 1 cut(s) | 258 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 176, 219, 245, 248, 452 |
| BseMI | GCAATG | 1 cut(s) | 716 |
| BseMII | CTCAG | 5 cut(s) | 66, 438, 654, 702, 713 |
| BseNI | ACTGG | 6 cut(s) | 89, 99, 181, 325, 373, 637 |
| BseSI | GKGCMC | 3 cut(s) | 213, 280, 383 |
| BseXI | GCAGC | 3 cut(s) | 89, 162, 165 |
| BseYI | CCCAGC | 1 cut(s) | 391 |
| BshFI | GGCC | 9 cut(s) | 169, 180, 223, 257, 297, 360, 365, 381, 396 |
| BshNI | GGYRCC | 1 cut(s) | 275 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 176, 219, 245, 248, 452 |
| BsmAI | GTCTC | 1 cut(s) | 707 |
| BsmI | GAATGC | 1 cut(s) | 306 |
| BsnI | GGCC | 9 cut(s) | 169, 180, 223, 257, 297, 360, 365, 381, 396 |
| Bsp120I | GGGCCC | 1 cut(s) | 379 |
| Bsp1286I | GDGCHC | 4 cut(s) | 213, 280, 383, 458 |
| Bsp1407I | TGTACA | 1 cut(s) | 126 |
| Bsp143I | GATC | 1 cut(s) | 726 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 325 |
| BspANI | GGCC | 9 cut(s) | 169, 180, 223, 257, 297, 360, 365, 381, 396 |
| BspCNI | CTCAG | 5 cut(s) | 65, 439, 655, 703, 714 |
| BspLI | GGNNCC | 5 cut(s) | 233, 277, 381, 409, 511 |
| BspPI | GGATC | 1 cut(s) | 734 |
| BspT107I | GGYRCC | 1 cut(s) | 275 |
| BsrDI | GCAATG | 1 cut(s) | 716 |
| BsrGI | TGTACA | 1 cut(s) | 126 |
| BsrI | ACTGG | 6 cut(s) | 89, 99, 181, 325, 373, 637 |
| BssECI | CCNNGG | 1 cut(s) | 397 |
| BssMI | GATC | 1 cut(s) | 726 |
| Bst2UI | CCWGG | 3 cut(s) | 362, 398, 687 |
| Bst4CI | ACNGT | 3 cut(s) | 40, 289, 418 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 64 |
| BstAUI | TGTACA | 1 cut(s) | 126 |
| BstC8I | GCNNGC | 1 cut(s) | 331 |
| BstDEI | CTNAG | 7 cut(s) | 52, 325, 447, 663, 711, 722, 740 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 217 |
| BstF5I | GGATG | 1 cut(s) | 258 |
| BstKTI | GATC | 1 cut(s) | 729 |
| BstMAI | GTCTC | 1 cut(s) | 707 |
| BstMBI | GATC | 1 cut(s) | 726 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 64, 186, 275, 303, 330, 500, 533 |
| BstNI | CCWGG | 3 cut(s) | 362, 398, 687 |
| BstNSI | RCATGY | 3 cut(s) | 62, 133, 698 |
| BstSCI | CCNGG | 3 cut(s) | 360, 396, 685 |
| BstSFI | CTRYAG | 1 cut(s) | 159 |
| BstSLI | GKGCMC | 3 cut(s) | 213, 280, 383 |
| BstV1I | GCAGC | 3 cut(s) | 89, 162, 165 |
| BsuRI | GGCC | 9 cut(s) | 169, 180, 223, 257, 297, 360, 365, 381, 396 |
| BtsCI | GGATG | 1 cut(s) | 258 |
| BtsIMutI | CAGTG | 8 cut(s) | 36, 60, 106, 174, 318, 624, 630, 699 |
| Cac8I | GCNNGC | 1 cut(s) | 331 |
| CaiI | CAGNNNCTG | 1 cut(s) | 446 |
| Cfr13I | GGNCC | 5 cut(s) | 364, 379, 380, 394, 510 |
| Csp6I | GTAC | 2 cut(s) | 62, 127 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 82, 117 |
| CviAII | CATG | 6 cut(s) | 59, 107, 130, 134, 538, 695 |
| CviQI | GTAC | 2 cut(s) | 62, 127 |
| DdeI | CTNAG | 7 cut(s) | 52, 325, 447, 663, 711, 722, 740 |
| DpnI | GATC | 1 cut(s) | 728 |
| DpnII | GATC | 1 cut(s) | 726 |
| DraIII | CACNNNGTG | 1 cut(s) | 248 |
| EaeI | YGGCCR | 2 cut(s) | 255, 358 |
| Eco24I | GRGCYC | 2 cut(s) | 383, 458 |
| Eco47I | GGWCC | 1 cut(s) | 510 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 217 |
| EcoO109I | RGGNCCY | 2 cut(s) | 380, 510 |
| EcoRII | CCWGG | 3 cut(s) | 360, 396, 685 |
| EcoT22I | ATGCAT | 1 cut(s) | 135 |
| EcoT38I | GRGCYC | 2 cut(s) | 383, 458 |
| FaeI | CATG | 6 cut(s) | 62, 110, 133, 137, 541, 698 |
| FatI | CATG | 6 cut(s) | 58, 106, 129, 133, 537, 694 |
| Fnu4HI | GCNGC | 3 cut(s) | 78, 151, 154 |
| FokI | GGATG | 1 cut(s) | 245 |
| FriOI | GRGCYC | 2 cut(s) | 383, 458 |
| Fsp4HI | GCNGC | 3 cut(s) | 78, 151, 154 |
| FspBI | CTAG | 1 cut(s) | 165 |
| GluI | GCNGC | 3 cut(s) | 78, 151, 154 |
| GsaI | CCCAGC | 1 cut(s) | 395 |
| HaeIII | GGCC | 9 cut(s) | 169, 180, 223, 257, 297, 360, 365, 381, 396 |
| Hin1II | CATG | 6 cut(s) | 62, 110, 133, 137, 541, 698 |
| HincII | GTYRAC | 2 cut(s) | 85, 692 |
| HindII | GTYRAC | 2 cut(s) | 85, 692 |
| HphI | GGTGA | 3 cut(s) | 6, 563, 572 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 692 |
| Hpy188III | TCNNGA | 2 cut(s) | 119, 345 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 692 |
| HpyAV | CCTTC | 3 cut(s) | 245, 466, 539 |
| HpyCH4III | ACNGT | 3 cut(s) | 40, 289, 418 |
| HpyCH4V | TGCA | 5 cut(s) | 58, 133, 218, 650, 736 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 64, 186, 275, 303, 330, 500, 533 |
| HpyF3I | CTNAG | 7 cut(s) | 52, 325, 447, 663, 711, 722, 740 |
| Hsp92II | CATG | 6 cut(s) | 62, 110, 133, 137, 541, 698 |
| Kzo9I | GATC | 1 cut(s) | 726 |
| Lsp1109I | GCAGC | 3 cut(s) | 89, 162, 165 |
| LweI | GCATC | 1 cut(s) | 745 |
| MaeI | CTAG | 1 cut(s) | 165 |
| MaeIII | GTNAC | 5 cut(s) | 560, 606, 619, 672, 697 |
| MalI | GATC | 1 cut(s) | 728 |
| MboI | GATC | 1 cut(s) | 726 |
| MboII | GAAGA | 1 cut(s) | 25 |
| MfeI | CAATTG | 1 cut(s) | 465 |
| MhlI | GDGCHC | 4 cut(s) | 213, 280, 383, 458 |
| MlsI | TGGCCA | 2 cut(s) | 257, 360 |
| MluCI | AATT | 5 cut(s) | 45, 114, 465, 591, 638 |
| MluNI | TGGCCA | 2 cut(s) | 257, 360 |
| Mox20I | TGGCCA | 2 cut(s) | 257, 360 |
| Mph1103I | ATGCAT | 1 cut(s) | 135 |
| MscI | TGGCCA | 2 cut(s) | 257, 360 |
| MseI | TTAA | 1 cut(s) | 569 |
| MslI | CAYNNNNRTG | 1 cut(s) | 536 |
| Msp20I | TGGCCA | 2 cut(s) | 257, 360 |
| MspA1I | CMGCKG | 1 cut(s) | 503 |
| MspR9I | CCNGG | 3 cut(s) | 362, 398, 687 |
| MunI | CAATTG | 1 cut(s) | 465 |
| Mva1269I | GAATGC | 1 cut(s) | 306 |
| MvaI | CCWGG | 3 cut(s) | 362, 398, 687 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 64, 186, 275, 303, 330, 500, 533 |
| NdeII | GATC | 1 cut(s) | 726 |
| NlaIII | CATG | 6 cut(s) | 62, 110, 133, 137, 541, 698 |
| NlaIV | GGNNCC | 5 cut(s) | 233, 277, 381, 409, 511 |
| NmuCI | GTSAC | 5 cut(s) | 560, 606, 619, 672, 697 |
| NsiI | ATGCAT | 1 cut(s) | 135 |
| NspI | RCATGY | 3 cut(s) | 62, 133, 698 |
| PciI | ACATGT | 1 cut(s) | 694 |
| PctI | GAATGC | 1 cut(s) | 306 |
| PflFI | GACNNNGTC | 1 cut(s) | 89 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 176, 248 |
| PkrI | GCNGC | 3 cut(s) | 79, 152, 155 |
| PpuMI | RGGWCCY | 1 cut(s) | 510 |
| PscI | ACATGT | 1 cut(s) | 694 |
| Psp5II | RGGWCCY | 1 cut(s) | 510 |
| Psp6I | CCWGG | 3 cut(s) | 360, 396, 685 |
| PspFI | CCCAGC | 1 cut(s) | 391 |
| PspGI | CCWGG | 3 cut(s) | 360, 396, 685 |
| PspN4I | GGNNCC | 5 cut(s) | 233, 277, 381, 409, 511 |
| PspOMI | GGGCCC | 1 cut(s) | 379 |
| PspPI | GGNCC | 5 cut(s) | 364, 379, 380, 394, 510 |
| PspPPI | RGGWCCY | 1 cut(s) | 510 |
| PstNI | CAGNNNCTG | 1 cut(s) | 446 |
| PsyI | GACNNNGTC | 1 cut(s) | 89 |
| PvuII | CAGCTG | 1 cut(s) | 503 |
| RsaI | GTAC | 2 cut(s) | 63, 128 |
| RsaNI | GTAC | 2 cut(s) | 62, 127 |
| RseI | CAYNNNNRTG | 1 cut(s) | 536 |
| SaqAI | TTAA | 1 cut(s) | 569 |
| SatI | GCNGC | 3 cut(s) | 78, 151, 154 |
| Sau3AI | GATC | 1 cut(s) | 726 |
| Sau96I | GGNCC | 5 cut(s) | 364, 379, 380, 394, 510 |
| ScrFI | CCNGG | 3 cut(s) | 362, 398, 687 |
| SduI | GDGCHC | 4 cut(s) | 213, 280, 383, 458 |
| SfaNI | GCATC | 1 cut(s) | 745 |
| SfcI | CTRYAG | 1 cut(s) | 159 |
| SinI | GGWCC | 1 cut(s) | 510 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 536 |
| SmlI | CTYRAG | 2 cut(s) | 119, 477 |
| SmoI | CTYRAG | 2 cut(s) | 119, 477 |
| Sse9I | AATT | 5 cut(s) | 45, 114, 465, 591, 638 |
| SspMI | CTAG | 1 cut(s) | 165 |
| StyD4I | CCNGG | 3 cut(s) | 360, 396, 685 |
| TaaI | ACNGT | 3 cut(s) | 40, 289, 418 |
| TasI | AATT | 5 cut(s) | 45, 114, 465, 591, 638 |
| TatI | WGTACW | 2 cut(s) | 61, 126 |
| Tru1I | TTAA | 1 cut(s) | 569 |
| Tru9I | TTAA | 1 cut(s) | 569 |
| TscAI | CASTG | 8 cut(s) | 43, 60, 106, 181, 325, 624, 637, 706 |
| TseFI | GTSAC | 5 cut(s) | 560, 606, 619, 672, 697 |
| TseI | GCWGC | 3 cut(s) | 77, 150, 153 |
| Tsp45I | GTSAC | 5 cut(s) | 560, 606, 619, 672, 697 |
| TspRI | CASTG | 8 cut(s) | 43, 60, 106, 181, 325, 624, 637, 706 |
| Tth111I | GACNNNGTC | 1 cut(s) | 89 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 176, 248 |
| VpaK11BI | GGWCC | 1 cut(s) | 510 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 217 |
| XapI | RAATTY | 1 cut(s) | 638 |
| XceI | RCATGY | 3 cut(s) | 62, 133, 698 |
| XspI | CTAG | 1 cut(s) | 165 |
| Zsp2I | ATGCAT | 1 cut(s) | 135 |