Rh4CG076700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005053
Physical Location & Seq
Forward (+)
13903261 .. 13904088
828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG076700.1

Sequence Viewer

Length: 828 bp
ATGCCTGTTTCCAAGAAGCATAATCAGCAAGAAAACTTCAGTATTCAATCCTCAAGTCTACGATACCAATCTTTCAAGATGTTCAAGCAGACAATTTCATCTGTATTCCTTGTGATGCTACTTGCAAACATGCACGCAGCATTTTCCGTTGATCCTGCAGCAGATGGCAAAGAGCCAGTTCTTGAATTATACATGCACGACATTCTTGGTGGCAGTAGCCCTACAGCTAGGCCAATCACTGGCTTGTTAGGTAACATCTACAGCAGTCAAGTCCCCTTCGCCAAGCCAATAGGATTCAATGTTCCAAGTGGTGGGGTGGCTGTCCCAAATGCCAATGGTGCCCTGCCGACTGTAAATGGTGCTACCGGTCTCCCACTAGGAACCGGCTTATCAGGCACAGCTTTTGCAGGAAATGCAGATCAACAGAACCAGAATGGCAATATCGCAACCCAGCTAGCAGCAGATGGATTGGGACTGGGATTCGGGACAATCACTGTCATTGACGACATACTGACCTCTAGCCCCGACTTGGGATCACAAACACTTGGAAAAGCTCAAGGAATTTATATGGCAAGCTCAGCAGATGGATCACGACAGCTGATGGCATTTACAGCTATGGTGGAAGGAGGGGAGTATAATGATAACCTCAACTTCTTTGGAGTGTACAAGATTGGAAGTACCATGTCACAGTTGTCAGTGACTGGTGGCACAGGTAAGTTTAAGAATGCCAATGGAATCGCAGAGTTGCGACCACTGATTCCTCCAGGCCAACATGCAACAGATGGTGCAGAAACTTTGTTGAGGGTTACTGTGCACTTAACCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

275

Amino Acids

28.62

Weight (kDa)

5.91

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 146 - 251 7.7e-24 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 2 cut(s) 239, 311
AccI GTMKAC 1 cut(s) 58
AclWI GGATC 3 cut(s) 146, 541, 595
AcsI RAATTY 1 cut(s) 561
AcuI CTGAAG 1 cut(s) 22
AfaI GTAC 2 cut(s) 665, 679
AfiI CCNNNNNNNGG 4 cut(s) 239, 311, 529, 530
AgeI ACCGGT 1 cut(s) 365
AgsI TTSAA 5 cut(s) 47, 76, 85, 185, 298
AjnI CCWGG 1 cut(s) 763
AloI GAACNNNNNNTCC 2 cut(s) 285, 317
AluBI AGCT 7 cut(s) 227, 401, 454, 554, 576, 598, 614
AluI AGCT 7 cut(s) 227, 401, 454, 554, 576, 598, 614
Alw21I GWGCWC 1 cut(s) 816
Alw26I GTCTC 1 cut(s) 374
Alw44I GTGCAC 1 cut(s) 812
AlwI GGATC 3 cut(s) 146, 541, 595
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 2 cut(s) 230, 766
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 3 cut(s) 137, 158, 458
ApoI RAATTY 1 cut(s) 561
AsiGI ACCGGT 1 cut(s) 365
AsuNHI GCTAGC 1 cut(s) 454
BaeGI GKGCMC 2 cut(s) 343, 816
BanI GGYRCC 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 816
BbvI GCAGC 3 cut(s) 149, 170, 470
BccI CCATC 5 cut(s) 158, 458, 578, 595, 776
BciT130I CCWGG 1 cut(s) 765
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 4 cut(s) 228, 377, 455, 519
BfmI CTRYAG 3 cut(s) 156, 222, 259
BisI GCNGC 3 cut(s) 138, 159, 459
BlpI GCTNAGC 1 cut(s) 577
BlsI GCNGC 3 cut(s) 139, 160, 460
Bme1390I CCNGG 1 cut(s) 765
BmiI GGNNCC 2 cut(s) 340, 382
BmrFI CCNGG 1 cut(s) 765
BmrI ACTGGG 1 cut(s) 485
BmsI GCATC 1 cut(s) 105
BmtI GCTAGC 1 cut(s) 458
BmuI ACTGGG 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 747
Bpu1102I GCTNAGC 1 cut(s) 577
BpuEI CTTGAG 2 cut(s) 37, 540
BsaBI GATNNNNATC 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 374
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 311, 529, 530
Bse118I RCCGGY 2 cut(s) 365, 383
Bse1I ACTGG 4 cut(s) 176, 244, 480, 706
Bse8I GATNNNNATC 1 cut(s) 67
BseBI CCWGG 1 cut(s) 765
BseJI GATNNNNATC 1 cut(s) 67
BseLI CCNNNNNNNGG 4 cut(s) 239, 311, 529, 530
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 4 cut(s) 176, 244, 480, 706
BseSI GKGCMC 2 cut(s) 343, 816
BseXI GCAGC 3 cut(s) 149, 170, 470
BseYI CCCAGC 1 cut(s) 450
BsgI GTGCAG 1 cut(s) 807
BshFI GGCC 2 cut(s) 232, 768
BshNI GGYRCC 1 cut(s) 338
BshTI ACCGGT 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 816
BsiSI CCGG 2 cut(s) 366, 384
BslFI GGGAC 4 cut(s) 257, 308, 486, 499
BslI CCNNNNNNNGG 4 cut(s) 239, 311, 529, 530
BsmAI GTCTC 1 cut(s) 374
BsmFI GGGAC 4 cut(s) 257, 308, 486, 499
BsmI GAATGC 1 cut(s) 730
BsnI GGCC 2 cut(s) 232, 768
Bso31I GGTCTC 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 343, 816
Bsp1407I TGTACA 1 cut(s) 663
Bsp143I GATC 4 cut(s) 151, 418, 533, 587
Bsp1720I GCTNAGC 1 cut(s) 577
BspANI GGCC 2 cut(s) 232, 768
BspCNI CTCAG 1 cut(s) 590
BspLI GGNNCC 2 cut(s) 340, 382
BspMAI CTGCAG 1 cut(s) 160
BspOI GCTAGC 1 cut(s) 458
BspPI GGATC 3 cut(s) 146, 541, 595
BspT107I GGYRCC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 374
BsrFI RCCGGY 2 cut(s) 365, 383
BsrGI TGTACA 1 cut(s) 663
BsrI ACTGG 4 cut(s) 176, 244, 480, 706
BssAI RCCGGY 2 cut(s) 365, 383
BssMI GATC 4 cut(s) 151, 418, 533, 587
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 4 cut(s) 352, 496, 690, 811
BstAPI GCANNNNNTGC 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 663
BstC8I GCNNGC 3 cut(s) 135, 456, 574
BstDEI CTNAG 1 cut(s) 577
BstKTI GATC 4 cut(s) 154, 421, 536, 590
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 4 cut(s) 151, 418, 533, 587
BstMWI GCNNNNNNNGC 6 cut(s) 25, 338, 393, 413, 578, 611
BstNI CCWGG 1 cut(s) 765
BstNSI RCATGY 3 cut(s) 133, 196, 776
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 3 cut(s) 156, 222, 259
BstSLI GKGCMC 2 cut(s) 343, 816
BstV1I GCAGC 3 cut(s) 149, 170, 470
BsuRI GGCC 2 cut(s) 232, 768
BtsIMutI CAGTG 4 cut(s) 237, 492, 702, 752
Cac8I GCNNGC 3 cut(s) 135, 456, 574
CaiI CAGNNNCTG 1 cut(s) 701
Cfr10I RCCGGY 2 cut(s) 365, 383
Csp6I GTAC 2 cut(s) 664, 678
CspAI ACCGGT 1 cut(s) 365
CviAII CATG 4 cut(s) 130, 193, 682, 773
CviQI GTAC 2 cut(s) 664, 678
DdeI CTNAG 1 cut(s) 577
DpnI GATC 4 cut(s) 153, 420, 535, 589
DpnII GATC 4 cut(s) 151, 418, 533, 587
Eco31I GGTCTC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 1 cut(s) 763
FaeI CATG 4 cut(s) 133, 196, 685, 776
FaqI GGGAC 4 cut(s) 257, 308, 486, 499
FatI CATG 4 cut(s) 129, 192, 681, 772
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 3 cut(s) 138, 159, 459
Fsp4HI GCNGC 3 cut(s) 138, 159, 459
FspBI CTAG 4 cut(s) 228, 377, 455, 519
GluI GCNGC 3 cut(s) 138, 159, 459
GsaI CCCAGC 1 cut(s) 454
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 2 cut(s) 232, 768
HapII CCGG 2 cut(s) 366, 384
Hin1II CATG 4 cut(s) 133, 196, 685, 776
HinfI GANTC 4 cut(s) 294, 480, 735, 757
HpaII CCGG 2 cut(s) 366, 384
Hpy166II GTNNAC 3 cut(s) 59, 664, 814
Hpy188III TCNNGA 4 cut(s) 76, 182, 484, 591
Hpy8I GTNNAC 3 cut(s) 59, 664, 814
HpyAV CCTTC 2 cut(s) 286, 617
HpyCH4III ACNGT 4 cut(s) 352, 496, 690, 811
HpyCH4V TGCA 9 cut(s) 125, 133, 158, 196, 407, 416, 776, 788, 814
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 338, 393, 413, 578, 611
HpyF3I CTNAG 1 cut(s) 577
Hsp92II CATG 4 cut(s) 133, 196, 685, 776
Kzo9I GATC 4 cut(s) 151, 418, 533, 587
Lsp1109I GCAGC 3 cut(s) 149, 170, 470
LweI GCATC 1 cut(s) 105
MaeI CTAG 4 cut(s) 228, 377, 455, 519
MaeIII GTNAC 4 cut(s) 251, 684, 697, 805
MalI GATC 4 cut(s) 153, 420, 535, 589
MboI GATC 4 cut(s) 151, 418, 533, 587
MhlI GDGCHC 2 cut(s) 343, 816
MluCI AATT 3 cut(s) 93, 185, 561
MnlI CCTC 6 cut(s) 61, 526, 620, 656, 771, 795
MseI TTAA 2 cut(s) 720, 818
MspA1I CMGCKG 1 cut(s) 598
MspI CCGG 2 cut(s) 366, 384
MspR9I CCNGG 1 cut(s) 765
Mva1269I GAATGC 1 cut(s) 730
MvaI CCWGG 1 cut(s) 765
MwoI GCNNNNNNNGC 6 cut(s) 25, 338, 393, 413, 578, 611
NdeII GATC 4 cut(s) 151, 418, 533, 587
NheI GCTAGC 1 cut(s) 454
NlaIII CATG 4 cut(s) 133, 196, 685, 776
NlaIV GGNNCC 2 cut(s) 340, 382
NmuCI GTSAC 2 cut(s) 684, 697
NspI RCATGY 3 cut(s) 133, 196, 776
PctI GAATGC 1 cut(s) 730
PfeI GAWTC 4 cut(s) 294, 480, 735, 757
PflMI CCANNNNNTGG 2 cut(s) 239, 311
PinAI ACCGGT 1 cut(s) 365
PkrI GCNGC 3 cut(s) 139, 160, 460
Psp6I CCWGG 1 cut(s) 763
PspFI CCCAGC 1 cut(s) 450
PspGI CCWGG 1 cut(s) 763
PspN4I GGNNCC 2 cut(s) 340, 382
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 598
RsaI GTAC 2 cut(s) 665, 679
RsaNI GTAC 2 cut(s) 664, 678
SaqAI TTAA 2 cut(s) 720, 818
SatI GCNGC 3 cut(s) 138, 159, 459
Sau3AI GATC 4 cut(s) 151, 418, 533, 587
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 2 cut(s) 343, 816
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 3 cut(s) 156, 222, 259
SmlI CTYRAG 2 cut(s) 52, 555
SmoI CTYRAG 2 cut(s) 52, 555
Sse9I AATT 3 cut(s) 93, 185, 561
SspMI CTAG 4 cut(s) 228, 377, 455, 519
StyD4I CCNGG 1 cut(s) 763
TaaI ACNGT 4 cut(s) 352, 496, 690, 811
TasI AATT 3 cut(s) 93, 185, 561
TatI WGTACW 1 cut(s) 663
TfiI GAWTC 4 cut(s) 294, 480, 735, 757
Tru1I TTAA 2 cut(s) 720, 818
Tru9I TTAA 2 cut(s) 720, 818
TscAI CASTG 4 cut(s) 244, 499, 702, 759
TseFI GTSAC 2 cut(s) 684, 697
TseI GCWGC 3 cut(s) 137, 158, 458
Tsp45I GTSAC 2 cut(s) 684, 697
TspDTI ATGAA 1 cut(s) 87
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 4 cut(s) 244, 499, 702, 759
Van91I CCANNNNNTGG 2 cut(s) 239, 311
VneI GTGCAC 1 cut(s) 812
XapI RAATTY 1 cut(s) 561
XceI RCATGY 3 cut(s) 133, 196, 776
XmiI GTMKAC 1 cut(s) 58
XspI CTAG 4 cut(s) 228, 377, 455, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.