Rh4DG065900

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005053
Physical Location & Seq
Forward (+)
9906451 .. 9907149
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG065900.1

Sequence Viewer

Length: 699 bp
ATGCACGCAGCATTTTCAGTTGATCCTGCAGCAGATGGCAAAGAGCCAGTTCTTGAATTATACATGCACGACATTCTTGGTGGCAGTAGCCCTACAGCTAGGCCGATCACTGGCTTGCTAGGTAACATCTACAGCAGTCAAGTCCCCTTCGCCAAGCCAATAGGATTCAATGTTCCAAGTGGTGGGGTGGCTGTCCCAAATGCCAATGGTGCCCTGCCGACTGTAAATGGTGCCACCGGTCTCCCACTAGGAACCGGCTTATCAGGTACAGCTTTTGCAGGAAATGCAGATCAACAGAACCAGAATGGCAATATCGCAACCCAGCTAGCAGCAGATGGATTGGGACTGGGATTCGGGACAATCACTGTCATTGACGACATACTGACCTCTAGCCCCGACTTGGGATCACAAACACTTGGAAAAGCTCAAGGAATTTATATGGCAAGCTCAGCAGATGGATCACGACAGCTGATGGCATTTACAGCTATGGTGGAAGGAGGGGAGTATAATGATAACCTGAACTTCTTTGGAGTGTACAAGATTGGAAGTACCATGTCACAGTTGTCAGTGACTGGTGGCACAGGTAAGTTTAAGAATGCCAATGGAATCGCAGAGTTGCGACCACTGATTCCTCCGGGCCAACATGCAACAGATGGTGCAGAAACTTTAATGAGGGTTACTGTGCACTTAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

232

Amino Acids

23.64

Weight (kDa)

4.85

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 103 - 208 6.5e-24 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 209, 230
AccB7I CCANNNNNTGG 1 cut(s) 182
AclWI GGATC 3 cut(s) 17, 412, 466
AcsI RAATTY 1 cut(s) 432
AfaI GTAC 3 cut(s) 268, 536, 550
AfiI CCNNNNNNNGG 4 cut(s) 110, 182, 400, 401
AgeI ACCGGT 1 cut(s) 236
AgsI TTSAA 2 cut(s) 56, 169
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
AluBI AGCT 7 cut(s) 98, 272, 325, 425, 447, 469, 485
AluI AGCT 7 cut(s) 98, 272, 325, 425, 447, 469, 485
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 1 cut(s) 245
Alw44I GTGCAC 1 cut(s) 683
AlwI GGATC 3 cut(s) 17, 412, 466
AlwNI CAGNNNCTG 1 cut(s) 572
AoxI GGCC 2 cut(s) 101, 637
ApaLI GTGCAC 1 cut(s) 683
ApeKI GCWGC 3 cut(s) 8, 29, 329
ApoI RAATTY 1 cut(s) 432
AsiGI ACCGGT 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 637
AsuC2I CCSGG 1 cut(s) 636
AsuNHI GCTAGC 1 cut(s) 325
BaeGI GKGCMC 2 cut(s) 214, 687
BanI GGYRCC 2 cut(s) 209, 230
Bbv12I GWGCWC 1 cut(s) 687
BbvI GCAGC 3 cut(s) 20, 41, 341
BccI CCATC 5 cut(s) 29, 329, 449, 466, 647
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 5 cut(s) 99, 119, 248, 326, 390
BfmI CTRYAG 3 cut(s) 27, 93, 130
BisI GCNGC 3 cut(s) 9, 30, 330
BlpI GCTNAGC 1 cut(s) 448
BlsI GCNGC 3 cut(s) 10, 31, 331
Bme1390I CCNGG 1 cut(s) 636
BmgT120I GGNCC 1 cut(s) 637
BmiI GGNNCC 3 cut(s) 211, 232, 253
BmrFI CCNGG 1 cut(s) 636
BmrI ACTGGG 1 cut(s) 356
BmtI GCTAGC 1 cut(s) 329
BmuI ACTGGG 1 cut(s) 356
Bpu1102I GCTNAGC 1 cut(s) 448
BpuEI CTTGAG 1 cut(s) 411
BpuMI CCSGG 1 cut(s) 636
BsaI GGTCTC 1 cut(s) 245
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 182, 400, 401
Bse118I RCCGGY 2 cut(s) 236, 254
Bse1I ACTGG 4 cut(s) 47, 115, 351, 577
BseLI CCNNNNNNNGG 4 cut(s) 110, 182, 400, 401
BseMII CTCAG 1 cut(s) 462
BseNI ACTGG 4 cut(s) 47, 115, 351, 577
BseSI GKGCMC 2 cut(s) 214, 687
BseXI GCAGC 3 cut(s) 20, 41, 341
BseYI CCCAGC 1 cut(s) 321
BsgI GTGCAG 1 cut(s) 678
BshFI GGCC 2 cut(s) 103, 639
BshNI GGYRCC 2 cut(s) 209, 230
BshTI ACCGGT 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 687
BsiSI CCGG 3 cut(s) 237, 255, 635
BslFI GGGAC 4 cut(s) 128, 179, 357, 370
BslI CCNNNNNNNGG 4 cut(s) 110, 182, 400, 401
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 4 cut(s) 128, 179, 357, 370
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 2 cut(s) 103, 639
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 214, 687
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 5 cut(s) 22, 105, 289, 404, 458
Bsp1720I GCTNAGC 1 cut(s) 448
BspANI GGCC 2 cut(s) 103, 639
BspCNI CTCAG 1 cut(s) 461
BspLI GGNNCC 3 cut(s) 211, 232, 253
BspMAI CTGCAG 1 cut(s) 31
BspOI GCTAGC 1 cut(s) 329
BspPI GGATC 3 cut(s) 17, 412, 466
BspT107I GGYRCC 2 cut(s) 209, 230
BspTNI GGTCTC 1 cut(s) 245
BsrFI RCCGGY 2 cut(s) 236, 254
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 4 cut(s) 47, 115, 351, 577
BssAI RCCGGY 2 cut(s) 236, 254
BssMI GATC 5 cut(s) 22, 105, 289, 404, 458
Bst4CI ACNGT 4 cut(s) 223, 367, 561, 682
BstAPI GCANNNNNTGC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 534
BstC8I GCNNGC 4 cut(s) 6, 116, 327, 445
BstDEI CTNAG 1 cut(s) 448
BstKTI GATC 5 cut(s) 25, 108, 292, 407, 461
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 5 cut(s) 22, 105, 289, 404, 458
BstMWI GCNNNNNNNGC 4 cut(s) 209, 284, 449, 482
BstNSI RCATGY 2 cut(s) 67, 647
BstSCI CCNGG 1 cut(s) 634
BstSFI CTRYAG 3 cut(s) 27, 93, 130
BstSLI GKGCMC 2 cut(s) 214, 687
BstV1I GCAGC 3 cut(s) 20, 41, 341
BsuRI GGCC 2 cut(s) 103, 639
BtsIMutI CAGTG 4 cut(s) 108, 363, 573, 623
Cac8I GCNNGC 4 cut(s) 6, 116, 327, 445
CaiI CAGNNNCTG 1 cut(s) 572
Cfr10I RCCGGY 2 cut(s) 236, 254
Cfr13I GGNCC 1 cut(s) 637
Csp6I GTAC 3 cut(s) 267, 535, 549
CspAI ACCGGT 1 cut(s) 236
CviAII CATG 3 cut(s) 64, 553, 644
CviQI GTAC 3 cut(s) 267, 535, 549
DdeI CTNAG 1 cut(s) 448
DpnI GATC 5 cut(s) 24, 107, 291, 406, 460
DpnII GATC 5 cut(s) 22, 105, 289, 404, 458
Eco31I GGTCTC 1 cut(s) 245
FaeI CATG 3 cut(s) 67, 556, 647
FaiI YATR 9 cut(s) 61, 65, 380, 438, 440, 488, 507, 554, 645
FaqI GGGAC 4 cut(s) 128, 179, 357, 370
FatI CATG 3 cut(s) 63, 552, 643
Fnu4HI GCNGC 3 cut(s) 9, 30, 330
Fsp4HI GCNGC 3 cut(s) 9, 30, 330
FspBI CTAG 5 cut(s) 99, 119, 248, 326, 390
GluI GCNGC 3 cut(s) 9, 30, 330
GsaI CCCAGC 1 cut(s) 325
HaeIII GGCC 2 cut(s) 103, 639
HapII CCGG 3 cut(s) 237, 255, 635
Hin1II CATG 3 cut(s) 67, 556, 647
HinfI GANTC 4 cut(s) 165, 351, 606, 628
HpaII CCGG 3 cut(s) 237, 255, 635
Hpy166II GTNNAC 2 cut(s) 535, 685
Hpy188III TCNNGA 3 cut(s) 53, 355, 462
Hpy8I GTNNAC 2 cut(s) 535, 685
HpyAV CCTTC 2 cut(s) 157, 488
HpyCH4III ACNGT 4 cut(s) 223, 367, 561, 682
HpyCH4V TGCA 8 cut(s) 4, 29, 67, 278, 287, 647, 659, 685
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 284, 449, 482
HpyF3I CTNAG 1 cut(s) 448
Hsp92II CATG 3 cut(s) 67, 556, 647
Kzo9I GATC 5 cut(s) 22, 105, 289, 404, 458
Lsp1109I GCAGC 3 cut(s) 20, 41, 341
MaeI CTAG 5 cut(s) 99, 119, 248, 326, 390
MaeIII GTNAC 4 cut(s) 122, 555, 568, 676
MalI GATC 5 cut(s) 24, 107, 291, 406, 460
MboI GATC 5 cut(s) 22, 105, 289, 404, 458
MhlI GDGCHC 2 cut(s) 214, 687
MluCI AATT 2 cut(s) 56, 432
MnlI CCTC 4 cut(s) 397, 491, 642, 666
MseI TTAA 3 cut(s) 591, 668, 689
MspA1I CMGCKG 1 cut(s) 469
MspI CCGG 3 cut(s) 237, 255, 635
MspR9I CCNGG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 601
MwoI GCNNNNNNNGC 4 cut(s) 209, 284, 449, 482
NciI CCSGG 1 cut(s) 636
NdeII GATC 5 cut(s) 22, 105, 289, 404, 458
NheI GCTAGC 1 cut(s) 325
NlaIII CATG 3 cut(s) 67, 556, 647
NlaIV GGNNCC 3 cut(s) 211, 232, 253
NmuCI GTSAC 2 cut(s) 555, 568
NspI RCATGY 2 cut(s) 67, 647
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 4 cut(s) 165, 351, 606, 628
PflMI CCANNNNNTGG 1 cut(s) 182
PinAI ACCGGT 1 cut(s) 236
PkrI GCNGC 3 cut(s) 10, 31, 331
PspFI CCCAGC 1 cut(s) 321
PspN4I GGNNCC 3 cut(s) 211, 232, 253
PspPI GGNCC 1 cut(s) 637
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 572
PvuII CAGCTG 1 cut(s) 469
RsaI GTAC 3 cut(s) 268, 536, 550
RsaNI GTAC 3 cut(s) 267, 535, 549
SaqAI TTAA 3 cut(s) 591, 668, 689
SatI GCNGC 3 cut(s) 9, 30, 330
Sau3AI GATC 5 cut(s) 22, 105, 289, 404, 458
Sau96I GGNCC 1 cut(s) 637
ScrFI CCNGG 1 cut(s) 636
SduI GDGCHC 2 cut(s) 214, 687
SfcI CTRYAG 3 cut(s) 27, 93, 130
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 2 cut(s) 56, 432
SspMI CTAG 5 cut(s) 99, 119, 248, 326, 390
StyD4I CCNGG 1 cut(s) 634
TaaI ACNGT 4 cut(s) 223, 367, 561, 682
TasI AATT 2 cut(s) 56, 432
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 4 cut(s) 165, 351, 606, 628
Tru1I TTAA 3 cut(s) 591, 668, 689
Tru9I TTAA 3 cut(s) 591, 668, 689
TscAI CASTG 4 cut(s) 115, 370, 573, 630
TseFI GTSAC 2 cut(s) 555, 568
TseI GCWGC 3 cut(s) 8, 29, 329
Tsp45I GTSAC 2 cut(s) 555, 568
TspRI CASTG 4 cut(s) 115, 370, 573, 630
Van91I CCANNNNNTGG 1 cut(s) 182
VneI GTGCAC 1 cut(s) 683
XapI RAATTY 1 cut(s) 432
XceI RCATGY 2 cut(s) 67, 647
XspI CTAG 5 cut(s) 99, 119, 248, 326, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.