RchiOBHm_Chr4g0396611

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005053
Physical Location & Seq
Forward (+)
12746922 .. 12747686
765 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36898

Sequence Viewer

Length: 753 bp
ATGGTCAAGCAATTATCACCAGTCTATTCCATTTTGCTCTCTATTCTATTTGCCTCAGTCCATGTACTACTGGTCAATGCAGCAGTTGACCCAGTCAGGGCCCTTGAACATGAACCAATTCTTGAGATGTACATGCATGATATTCTTGGGGGCAGCAGCCCAACAGCTAGGCCTATAACTGGTCTGCTAGGCAACATCTATGGAGGTCAAGTTCCCTTTGCAAAGCCAATAGGGTTCCTTCCCCCACAAGGTGGTGTCCCCATCCCCAATGCTAATGGTGCCCTCCCTAGTGTCAATGGCATCAATGGGATCCCTCTTGGAACCGGCTTGTCCGGCACAACGTTTTCGGGAAAGCCCATTGGGCAGGCCCAGACCCAGTTGGGCCCTGATGGTCTGAGCCTGGGCTTCGGCACCATCACCGTCATTGATGACATTCTCACCACAGCACCTGAGCTAGGGTCACAGAATATTGGTAAAGCACAAGGGGTTTATGTGGCAAGTTCAGCAGATGGGTCAAGACAGATGATGGCATTCACTGCTATGATTGAAGGAGGTGAGTATGGTGATAGTCTCAACTTCTATGGTGTGTTTAAGATTGGAAGCCCAATGTCACATTTGTCTGTGACTGGAGGGACTGGGAAGTTCAAGAGTGCTAGTGGTTTTGCTGAGGTGAGATCACTAATCCCTCCTGGTCAACATATTACTGATGGTGCAGAGACTCTGCTGAGGATCATCGTACATCTAAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

250

Amino Acids

25.81

Weight (kDa)

5.81

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 131 - 226 9.8e-24 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 278, 410
AccB7I CCANNNNNTGG 1 cut(s) 251
AclI AACGTT 1 cut(s) 341
AclWI GGATC 3 cut(s) 304, 317, 737
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 3 cut(s) 66, 131, 738
AfiI CCNNNNNNNGG 5 cut(s) 97, 179, 248, 251, 455
AgsI TTSAA 3 cut(s) 107, 548, 646
AjnI CCWGG 2 cut(s) 399, 688
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
AluBI AGCT 3 cut(s) 167, 454, 747
AluI AGCT 3 cut(s) 167, 454, 747
Alw26I GTCTC 2 cut(s) 575, 710
AlwI GGATC 3 cut(s) 304, 317, 737
AlwNI CAGNNNCTG 1 cut(s) 449
AoxI GGCC 4 cut(s) 99, 170, 366, 382
ApaI GGGCCC 2 cut(s) 103, 386
ApeKI GCWGC 3 cut(s) 80, 153, 156
Asp700I GAANNNNTTC 1 cut(s) 117
AspS9I GGNCC 5 cut(s) 99, 100, 367, 382, 383
AsuHPI GGTGA 6 cut(s) 9, 409, 430, 566, 575, 682
BaeGI GKGCMC 3 cut(s) 103, 283, 386
BamHI GGATCC 1 cut(s) 309
BanI GGYRCC 2 cut(s) 278, 410
BanII GRGCYC 2 cut(s) 103, 386
BbvCI CCTCAGC 2 cut(s) 666, 725
BbvI GCAGC 3 cut(s) 92, 165, 168
BccI CCATC 6 cut(s) 269, 383, 422, 503, 520, 701
BciT130I CCWGG 2 cut(s) 401, 690
BcoDI GTCTC 2 cut(s) 575, 710
BfaI CTAG 5 cut(s) 168, 188, 288, 455, 654
BglI GCCNNNNNGGC 1 cut(s) 361
BisI GCNGC 3 cut(s) 81, 154, 157
BlsI GCNGC 3 cut(s) 82, 155, 158
Bme1390I CCNGG 2 cut(s) 401, 690
BmgT120I GGNCC 5 cut(s) 99, 100, 367, 382, 383
BmiI GGNNCC 7 cut(s) 101, 236, 280, 311, 322, 384, 412
BmrFI CCNGG 2 cut(s) 401, 690
BmrI ACTGGG 3 cut(s) 86, 370, 645
BmsI GCATC 1 cut(s) 309
BmuI ACTGGG 3 cut(s) 86, 370, 645
BpmI CTGGAG 1 cut(s) 648
Bpu10I CCTNAGC 3 cut(s) 450, 666, 725
BpuEI CTTGAG 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 179, 248, 251, 455
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 7 cut(s) 20, 75, 92, 184, 376, 631, 640
BseBI CCWGG 2 cut(s) 401, 690
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 5 cut(s) 97, 179, 248, 251, 455
BseMII CTCAG 5 cut(s) 69, 386, 441, 657, 716
BseNI ACTGG 7 cut(s) 20, 75, 92, 184, 376, 631, 640
BseSI GKGCMC 3 cut(s) 103, 283, 386
BseXI GCAGC 3 cut(s) 92, 165, 168
BsgI GTGCAG 1 cut(s) 732
BshFI GGCC 4 cut(s) 101, 172, 368, 384
BshNI GGYRCC 2 cut(s) 278, 410
BsiSI CCGG 2 cut(s) 324, 333
BslFI GGGAC 2 cut(s) 242, 646
BslI CCNNNNNNNGG 5 cut(s) 97, 179, 248, 251, 455
BsmAI GTCTC 2 cut(s) 575, 710
BsmFI GGGAC 2 cut(s) 242, 646
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 4 cut(s) 101, 172, 368, 384
Bsp120I GGGCCC 2 cut(s) 99, 382
Bsp1286I GDGCHC 3 cut(s) 103, 283, 386
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 3 cut(s) 309, 674, 729
BspANI GGCC 4 cut(s) 101, 172, 368, 384
BspCNI CTCAG 5 cut(s) 68, 387, 442, 658, 717
BspLI GGNNCC 7 cut(s) 101, 236, 280, 311, 322, 384, 412
BspPI GGATC 3 cut(s) 304, 317, 737
BspT107I GGYRCC 2 cut(s) 278, 410
BsrFI RCCGGY 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 7 cut(s) 20, 75, 92, 184, 376, 631, 640
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 3 cut(s) 309, 674, 729
Bst2UI CCWGG 2 cut(s) 401, 690
Bst4CI ACNGT 1 cut(s) 421
BstAPI GCANNNNNTGC 1 cut(s) 536
BstAUI TGTACA 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 6 cut(s) 55, 395, 450, 666, 725, 743
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 1 cut(s) 261
BstKTI GATC 3 cut(s) 312, 677, 732
BstMAI GTCTC 2 cut(s) 575, 710
BstMBI GATC 3 cut(s) 309, 674, 729
BstMWI GCNNNNNNNGC 5 cut(s) 278, 333, 361, 503, 536
BstNI CCWGG 2 cut(s) 401, 690
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 399, 688
BstSLI GKGCMC 3 cut(s) 103, 283, 386
BstV1I GCAGC 3 cut(s) 92, 165, 168
BstX2I RGATCY 1 cut(s) 309
BstYI RGATCY 1 cut(s) 309
BsuRI GGCC 4 cut(s) 101, 172, 368, 384
BtsCI GGATG 1 cut(s) 261
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 366
CaiI CAGNNNCTG 1 cut(s) 449
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 5 cut(s) 99, 100, 367, 382, 383
Csp6I GTAC 3 cut(s) 65, 130, 737
CviAII CATG 4 cut(s) 62, 110, 133, 137
CviQI GTAC 3 cut(s) 65, 130, 737
DdeI CTNAG 6 cut(s) 55, 395, 450, 666, 725, 743
DpnI GATC 3 cut(s) 311, 676, 731
DpnII GATC 3 cut(s) 309, 674, 729
DraIII CACNNNGTG 1 cut(s) 251
Eco147I AGGCCT 1 cut(s) 172
Eco24I GRGCYC 2 cut(s) 103, 386
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoO109I RGGNCCY 3 cut(s) 99, 100, 383
EcoRII CCWGG 2 cut(s) 399, 688
EcoT22I ATGCAT 1 cut(s) 138
EcoT38I GRGCYC 2 cut(s) 103, 386
FaeI CATG 4 cut(s) 65, 113, 136, 140
FaqI GGGAC 2 cut(s) 242, 646
FatI CATG 4 cut(s) 61, 109, 132, 136
Fnu4HI GCNGC 3 cut(s) 81, 154, 157
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 2 cut(s) 103, 386
Fsp4HI GCNGC 3 cut(s) 81, 154, 157
FspBI CTAG 5 cut(s) 168, 188, 288, 455, 654
GluI GCNGC 3 cut(s) 81, 154, 157
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 4 cut(s) 101, 172, 368, 384
HapII CCGG 2 cut(s) 324, 333
Hin1II CATG 4 cut(s) 65, 113, 136, 140
HincII GTYRAC 2 cut(s) 88, 695
HindII GTYRAC 2 cut(s) 88, 695
HinfI GANTC 1 cut(s) 718
HpaII CCGG 2 cut(s) 324, 333
HphI GGTGA 6 cut(s) 9, 409, 430, 566, 575, 682
Hpy166II GTNNAC 2 cut(s) 88, 695
Hpy188I TCNGA 1 cut(s) 396
Hpy188III TCNNGA 4 cut(s) 122, 348, 516, 646
Hpy8I GTNNAC 2 cut(s) 88, 695
HpyAV CCTTC 2 cut(s) 248, 542
HpyCH4III ACNGT 1 cut(s) 421
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 4 cut(s) 80, 136, 221, 713
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 333, 361, 503, 536
HpyF3I CTNAG 6 cut(s) 55, 395, 450, 666, 725, 743
HpySE526I ACGT 1 cut(s) 341
Hsp92II CATG 4 cut(s) 65, 113, 136, 140
Kzo9I GATC 3 cut(s) 309, 674, 729
Lsp1109I GCAGC 3 cut(s) 92, 165, 168
LweI GCATC 1 cut(s) 309
MaeI CTAG 5 cut(s) 168, 188, 288, 455, 654
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 3 cut(s) 459, 609, 622
MalI GATC 3 cut(s) 311, 676, 731
MboI GATC 3 cut(s) 309, 674, 729
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 3 cut(s) 103, 283, 386
MluCI AATT 2 cut(s) 11, 117
MlyI GAGTC 1 cut(s) 712
MnlI CCTC 9 cut(s) 64, 197, 293, 324, 545, 623, 661, 696, 720
Mph1103I ATGCAT 1 cut(s) 138
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 1 cut(s) 539
MspI CCGG 2 cut(s) 324, 333
MspR9I CCNGG 2 cut(s) 401, 690
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 401, 690
MwoI GCNNNNNNNGC 5 cut(s) 278, 333, 361, 503, 536
NdeII GATC 3 cut(s) 309, 674, 729
NlaIII CATG 4 cut(s) 65, 113, 136, 140
NlaIV GGNNCC 7 cut(s) 101, 236, 280, 311, 322, 384, 412
NmuCI GTSAC 3 cut(s) 459, 609, 622
NsiI ATGCAT 1 cut(s) 138
NspI RCATGY 1 cut(s) 136
PceI AGGCCT 1 cut(s) 172
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 117
PflFI GACNNNGTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 3 cut(s) 82, 155, 158
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
Psp1406I AACGTT 1 cut(s) 341
Psp6I CCWGG 2 cut(s) 399, 688
PspGI CCWGG 2 cut(s) 399, 688
PspN4I GGNNCC 7 cut(s) 101, 236, 280, 311, 322, 384, 412
PspOMI GGGCCC 2 cut(s) 99, 382
PspPI GGNCC 5 cut(s) 99, 100, 367, 382, 383
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 1 cut(s) 309
PsyI GACNNNGTC 1 cut(s) 92
RsaI GTAC 3 cut(s) 66, 131, 738
RsaNI GTAC 3 cut(s) 65, 130, 737
RseI CAYNNNNRTG 1 cut(s) 539
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 3 cut(s) 81, 154, 157
Sau3AI GATC 3 cut(s) 309, 674, 729
Sau96I GGNCC 5 cut(s) 99, 100, 367, 382, 383
SchI GAGTC 1 cut(s) 712
ScrFI CCNGG 2 cut(s) 401, 690
SduI GDGCHC 3 cut(s) 103, 283, 386
SetI ASST 9 cut(s) 169, 208, 253, 344, 451, 456, 556, 672, 749
SfaNI GCATC 1 cut(s) 309
SmiMI CAYNNNNRTG 1 cut(s) 539
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 11, 117
SseBI AGGCCT 1 cut(s) 172
SspI AATATT 1 cut(s) 469
SspMI CTAG 5 cut(s) 168, 188, 288, 455, 654
StuI AGGCCT 1 cut(s) 172
StyD4I CCNGG 2 cut(s) 399, 688
TaaI ACNGT 1 cut(s) 421
TaiI ACGT 1 cut(s) 344
TasI AATT 2 cut(s) 11, 117
TatI WGTACW 2 cut(s) 64, 129
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 1 cut(s) 541
TseFI GTSAC 3 cut(s) 459, 609, 622
TseI GCWGC 3 cut(s) 80, 153, 156
Tsp45I GTSAC 3 cut(s) 459, 609, 622
TspDTI ATGAA 1 cut(s) 126
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 92
Van91I CCANNNNNTGG 1 cut(s) 251
XagI CCTNNNNNAGG 1 cut(s) 453
XceI RCATGY 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 5 cut(s) 168, 188, 288, 455, 654
Zsp2I ATGCAT 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.