RchiOBHm_Chr4g0396621

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005053
Physical Location & Seq
Forward (+)
12749615 .. 12750609
995 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36899

Sequence Viewer

Length: 750 bp
ATGTTCAAGCAGACAATTTCATCTGTTTTCCTTGTGATGCTACTTGCAAATATGCACGCAGCATTTTCAGTTGATCCTGCAGCAGATGGCAAAGAGCCAGTTCTTGAATTATACATGCATGACATTCTTGGTGGCAGTAGCCCTACAGCTAGGCCGATCACTGGCTTGCTAGGTAACATCTACAGCAGTCAAGTCCCCTTCGCCAAGCCAATAGGATTCAATGTTCCAAGTGGTGGGGTGGCTGTCCCAAATGCCAATGGTGCCCTGCCGACTGTAAATGGTGCCACCGGTCTCCCACTAGGAACCGGCTTATCAGGTACAGCTTTTGCAGGAAATGCAGATCAACAGAACCAGAATGGCAATATCGCAACCCAGCTAGCAGCAGATGGACTGGGACTGGGATTCGGGACAATCACTGTCATTGACGACATACTGACCTCTAGCCCCGACTTGGGATCACAAACACTTGGAAAAGCTCAAGGAATTTATATGGCAAGCTCGGCAGATGGATCACGACAGCTGATGGCATTTACAGCTATGGTGGAAGGAGGGGAGTATAATGATAACCTCAACTTCTTTGGAGTGTACAAGATTGGAAGTACCATGTCACAGTTGTCAGTGACCAGTGGCACAGGTAAGTTTAAGAATGCCAATGGAATCGCAGAGCTGCGACCACTGATTCCTCCAGGCCAACATGCAACAGATGGTGCAGAAACTTTGCTGAGGGTTACTGTGCACTTAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

249

Amino Acids

25.58

Weight (kDa)

5.01

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 120 - 225 5.6e-23 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 260, 281
AccB7I CCANNNNNTGG 1 cut(s) 233
AclWI GGATC 3 cut(s) 68, 463, 517
AcsI RAATTY 1 cut(s) 483
AfaI GTAC 3 cut(s) 319, 587, 601
AfiI CCNNNNNNNGG 4 cut(s) 161, 233, 451, 452
AgeI ACCGGT 1 cut(s) 287
AgsI TTSAA 3 cut(s) 7, 107, 220
AjnI CCWGG 1 cut(s) 685
AloI GAACNNNNNNTCC 2 cut(s) 207, 239
AluBI AGCT 8 cut(s) 149, 323, 376, 476, 498, 520, 536, 667
AluI AGCT 8 cut(s) 149, 323, 376, 476, 498, 520, 536, 667
Alw21I GWGCWC 1 cut(s) 738
Alw26I GTCTC 1 cut(s) 296
Alw44I GTGCAC 1 cut(s) 734
AlwI GGATC 3 cut(s) 68, 463, 517
AoxI GGCC 2 cut(s) 152, 688
ApaLI GTGCAC 1 cut(s) 734
ApeKI GCWGC 4 cut(s) 59, 80, 380, 667
ApoI RAATTY 1 cut(s) 483
AsiGI ACCGGT 1 cut(s) 287
AsuNHI GCTAGC 1 cut(s) 376
BaeGI GKGCMC 2 cut(s) 265, 738
BanI GGYRCC 2 cut(s) 260, 281
Bbv12I GWGCWC 1 cut(s) 738
BbvCI CCTCAGC 1 cut(s) 722
BbvI GCAGC 4 cut(s) 71, 92, 392, 654
BccI CCATC 5 cut(s) 80, 380, 500, 517, 698
BciT130I CCWGG 1 cut(s) 687
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 5 cut(s) 150, 170, 299, 377, 441
BfmI CTRYAG 3 cut(s) 78, 144, 181
BisI GCNGC 4 cut(s) 60, 81, 381, 668
BlsI GCNGC 4 cut(s) 61, 82, 382, 669
Bme1390I CCNGG 1 cut(s) 687
BmiI GGNNCC 3 cut(s) 262, 283, 304
BmrFI CCNGG 1 cut(s) 687
BmrI ACTGGG 2 cut(s) 401, 407
BmsI GCATC 1 cut(s) 27
BmtI GCTAGC 1 cut(s) 380
BmuI ACTGGG 2 cut(s) 401, 407
BpmI CTGGAG 1 cut(s) 669
Bpu10I CCTNAGC 1 cut(s) 722
BpuEI CTTGAG 1 cut(s) 462
BsaI GGTCTC 1 cut(s) 296
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 233, 451, 452
Bse118I RCCGGY 2 cut(s) 287, 305
Bse1I ACTGG 5 cut(s) 98, 166, 396, 402, 624
BseBI CCWGG 1 cut(s) 687
BseLI CCNNNNNNNGG 4 cut(s) 161, 233, 451, 452
BseMII CTCAG 1 cut(s) 713
BseNI ACTGG 5 cut(s) 98, 166, 396, 402, 624
BseSI GKGCMC 2 cut(s) 265, 738
BseXI GCAGC 4 cut(s) 71, 92, 392, 654
BseYI CCCAGC 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 729
BshFI GGCC 2 cut(s) 154, 690
BshNI GGYRCC 2 cut(s) 260, 281
BshTI ACCGGT 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 738
BsiSI CCGG 2 cut(s) 288, 306
BslFI GGGAC 4 cut(s) 179, 230, 408, 421
BslI CCNNNNNNNGG 4 cut(s) 161, 233, 451, 452
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 4 cut(s) 179, 230, 408, 421
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 2 cut(s) 154, 690
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 265, 738
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 5 cut(s) 73, 156, 340, 455, 509
BspANI GGCC 2 cut(s) 154, 690
BspCNI CTCAG 1 cut(s) 714
BspLI GGNNCC 3 cut(s) 262, 283, 304
BspMAI CTGCAG 1 cut(s) 82
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 3 cut(s) 68, 463, 517
BspT107I GGYRCC 2 cut(s) 260, 281
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 2 cut(s) 287, 305
BsrGI TGTACA 1 cut(s) 585
BsrI ACTGG 5 cut(s) 98, 166, 396, 402, 624
BssAI RCCGGY 2 cut(s) 287, 305
BssMI GATC 5 cut(s) 73, 156, 340, 455, 509
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 4 cut(s) 274, 418, 612, 733
BstAPI GCANNNNNTGC 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 585
BstC8I GCNNGC 4 cut(s) 57, 167, 378, 496
BstDEI CTNAG 1 cut(s) 722
BstKTI GATC 5 cut(s) 76, 159, 343, 458, 512
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 5 cut(s) 73, 156, 340, 455, 509
BstMWI GCNNNNNNNGC 4 cut(s) 260, 335, 500, 533
BstNI CCWGG 1 cut(s) 687
BstNSI RCATGY 2 cut(s) 118, 698
BstSCI CCNGG 1 cut(s) 685
BstSFI CTRYAG 3 cut(s) 78, 144, 181
BstSLI GKGCMC 2 cut(s) 265, 738
BstV1I GCAGC 4 cut(s) 71, 92, 392, 654
BsuRI GGCC 2 cut(s) 154, 690
BtsIMutI CAGTG 5 cut(s) 159, 414, 624, 631, 674
Cac8I GCNNGC 4 cut(s) 57, 167, 378, 496
Cfr10I RCCGGY 2 cut(s) 287, 305
Csp6I GTAC 3 cut(s) 318, 586, 600
CspAI ACCGGT 1 cut(s) 287
CviAII CATG 4 cut(s) 115, 119, 604, 695
CviQI GTAC 3 cut(s) 318, 586, 600
DdeI CTNAG 1 cut(s) 722
DpnI GATC 5 cut(s) 75, 158, 342, 457, 511
DpnII GATC 5 cut(s) 73, 156, 340, 455, 509
Eco31I GGTCTC 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 685
EcoT22I ATGCAT 1 cut(s) 120
FaeI CATG 4 cut(s) 118, 122, 607, 698
FaqI GGGAC 4 cut(s) 179, 230, 408, 421
FatI CATG 4 cut(s) 114, 118, 603, 694
Fnu4HI GCNGC 4 cut(s) 60, 81, 381, 668
Fsp4HI GCNGC 4 cut(s) 60, 81, 381, 668
FspBI CTAG 5 cut(s) 150, 170, 299, 377, 441
GluI GCNGC 4 cut(s) 60, 81, 381, 668
GsaI CCCAGC 1 cut(s) 376
GsuI CTGGAG 1 cut(s) 669
HaeIII GGCC 2 cut(s) 154, 690
HapII CCGG 2 cut(s) 288, 306
Hin1II CATG 4 cut(s) 118, 122, 607, 698
HinfI GANTC 4 cut(s) 216, 402, 657, 679
HpaII CCGG 2 cut(s) 288, 306
Hpy166II GTNNAC 2 cut(s) 586, 736
Hpy188III TCNNGA 3 cut(s) 104, 406, 513
Hpy8I GTNNAC 2 cut(s) 586, 736
HpyAV CCTTC 2 cut(s) 208, 539
HpyCH4III ACNGT 4 cut(s) 274, 418, 612, 733
HpyCH4V TGCA 9 cut(s) 47, 55, 80, 118, 329, 338, 698, 710, 736
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 335, 500, 533
HpyF3I CTNAG 1 cut(s) 722
Hsp92II CATG 4 cut(s) 118, 122, 607, 698
Kzo9I GATC 5 cut(s) 73, 156, 340, 455, 509
Lsp1109I GCAGC 4 cut(s) 71, 92, 392, 654
LweI GCATC 1 cut(s) 27
MaeI CTAG 5 cut(s) 150, 170, 299, 377, 441
MaeIII GTNAC 4 cut(s) 173, 606, 619, 727
MalI GATC 5 cut(s) 75, 158, 342, 457, 511
MboI GATC 5 cut(s) 73, 156, 340, 455, 509
MhlI GDGCHC 2 cut(s) 265, 738
MluCI AATT 3 cut(s) 15, 107, 483
MnlI CCTC 5 cut(s) 448, 542, 578, 693, 717
Mph1103I ATGCAT 1 cut(s) 120
MseI TTAA 2 cut(s) 642, 740
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 2 cut(s) 288, 306
MspR9I CCNGG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 1 cut(s) 687
MwoI GCNNNNNNNGC 4 cut(s) 260, 335, 500, 533
NdeII GATC 5 cut(s) 73, 156, 340, 455, 509
NheI GCTAGC 1 cut(s) 376
NlaIII CATG 4 cut(s) 118, 122, 607, 698
NlaIV GGNNCC 3 cut(s) 262, 283, 304
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 2 cut(s) 606, 619
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 2 cut(s) 118, 698
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 4 cut(s) 216, 402, 657, 679
PflMI CCANNNNNTGG 1 cut(s) 233
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 4 cut(s) 61, 82, 382, 669
Psp6I CCWGG 1 cut(s) 685
PspFI CCCAGC 1 cut(s) 372
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 3 cut(s) 262, 283, 304
PstI CTGCAG 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 3 cut(s) 319, 587, 601
RsaNI GTAC 3 cut(s) 318, 586, 600
SaqAI TTAA 2 cut(s) 642, 740
SatI GCNGC 4 cut(s) 60, 81, 381, 668
Sau3AI GATC 5 cut(s) 73, 156, 340, 455, 509
ScrFI CCNGG 1 cut(s) 687
SduI GDGCHC 2 cut(s) 265, 738
SfaNI GCATC 1 cut(s) 27
SfcI CTRYAG 3 cut(s) 78, 144, 181
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
Sse9I AATT 3 cut(s) 15, 107, 483
SspMI CTAG 5 cut(s) 150, 170, 299, 377, 441
StyD4I CCNGG 1 cut(s) 685
TaaI ACNGT 4 cut(s) 274, 418, 612, 733
TasI AATT 3 cut(s) 15, 107, 483
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 4 cut(s) 216, 402, 657, 679
Tru1I TTAA 2 cut(s) 642, 740
Tru9I TTAA 2 cut(s) 642, 740
TscAI CASTG 5 cut(s) 166, 421, 624, 631, 681
TseFI GTSAC 2 cut(s) 606, 619
TseI GCWGC 4 cut(s) 59, 80, 380, 667
Tsp45I GTSAC 2 cut(s) 606, 619
TspDTI ATGAA 1 cut(s) 9
TspRI CASTG 5 cut(s) 166, 421, 624, 631, 681
Van91I CCANNNNNTGG 1 cut(s) 233
VneI GTGCAC 1 cut(s) 734
XapI RAATTY 1 cut(s) 483
XceI RCATGY 2 cut(s) 118, 698
XspI CTAG 5 cut(s) 150, 170, 299, 377, 441
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.