AT3G50685

peptidase inhibitor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008544
Physical Location & Seq
Reverse (-)
18834155 .. 18834834
680 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50685.1

Sequence Viewer

Length: 477 bp
ATGCTTCTTCTATCTCCGATCTCTGCCTCTCTTCCACCGTCGTTTCACCGGGGTAATTTGATCCGGCGATCAATTAAGCCGTTAGGTAGGGTTGTTGCAAAGGCCAAGGACAACACCGACACTGGTGGATTTCTGGAGACGGCAGCTATAGCCGGTAGTTTAGTGTCGACGCCGGTTATTGGATGGTCACTCTACACTTTGAAGACCACGGGATGTGGCTTACCTCCCGGACCGGCCGGTTTAATCGGAGCGTTGGAAGGTGTGAGCTACTTGGTGGTGGTTGGCATCGTGGGCTGGTCTTTGTACACTAAAACAAAAACTGGGTCAGGTCTGCCAAATGGGCCATTTGGTTTGTTGGGTGCGGTCGAGGGTTTATCGTATCTGTCGGTTCTAGCCATTCTTGTGGTGTTTGGTATTCAGTTCTTGGATAATGGGTCGGTTCCAGGTCCACTTCCTAGTGACCAGTGTTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

16.14

Weight (kDa)

9.03

Isoelectric Point (pI)

23.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 167
AciI CCGC 1 cut(s) 362
AclWI GGATC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 234
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 1 cut(s) 202
AjnI CCWGG 1 cut(s) 442
AluBI AGCT 2 cut(s) 146, 267
AluI AGCT 2 cut(s) 146, 267
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 55
AoxI GGCC 3 cut(s) 102, 234, 341
ApeKI GCWGC 1 cut(s) 143
ArsI GACNNNNNNTTYG 3 cut(s) 308, 340, 452
AspS9I GGNCC 3 cut(s) 230, 341, 446
AsuC2I CCSGG 2 cut(s) 50, 228
AsuHPI GGTGA 1 cut(s) 38
AvaII GGWCC 2 cut(s) 230, 446
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 155
BccI CCATC 1 cut(s) 177
BceAI ACGGC 2 cut(s) 64, 156
BciT130I CCWGG 1 cut(s) 444
BcnI CCSGG 2 cut(s) 50, 228
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 392, 456
BfmI CTRYAG 1 cut(s) 147
BglI GCCNNNNNGGC 1 cut(s) 340
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme1390I CCNGG 3 cut(s) 50, 228, 444
Bme18I GGWCC 2 cut(s) 230, 446
BmgT120I GGNCC 3 cut(s) 230, 341, 446
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 3 cut(s) 50, 228, 444
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 1 cut(s) 294
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 155
BpuMI CCSGG 2 cut(s) 50, 228
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 49, 105, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse118I RCCGGY 4 cut(s) 152, 172, 232, 236
Bse1I ACTGG 3 cut(s) 127, 325, 463
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 49, 105, 207
BseGI GGATG 2 cut(s) 188, 218
BseLI CCNNNNNNNGG 1 cut(s) 179
BseNI ACTGG 3 cut(s) 127, 325, 463
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 1 cut(s) 155
Bsh1285I CGRYCG 2 cut(s) 237, 366
BshFI GGCC 3 cut(s) 104, 236, 343
BsiEI CGRYCG 2 cut(s) 237, 366
BsiSI CCGG 7 cut(s) 49, 64, 153, 173, 228, 233, 237
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 131
BsmBI CGTCTC 1 cut(s) 131
BsnI GGCC 3 cut(s) 104, 236, 343
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 3 cut(s) 18, 60, 68
BspACI CCGC 1 cut(s) 362
BspANI GGCC 3 cut(s) 104, 236, 343
BspLI GGNNCC 1 cut(s) 441
BspPI GGATC 1 cut(s) 55
BsrFI RCCGGY 4 cut(s) 152, 172, 232, 236
BsrGI TGTACA 1 cut(s) 303
BsrI ACTGG 3 cut(s) 127, 325, 463
BssAI RCCGGY 4 cut(s) 152, 172, 232, 236
BssECI CCNNGG 3 cut(s) 49, 105, 207
BssMI GATC 3 cut(s) 18, 60, 68
BssNI GRCGYC 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 1 cut(s) 39
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 170
BstAUI TGTACA 1 cut(s) 303
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 188, 218
BstKTI GATC 3 cut(s) 21, 63, 71
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 18, 60, 68
BstMCI CGRYCG 2 cut(s) 237, 366
BstMWI GCNNNNNNNGC 3 cut(s) 149, 291, 340
BstNI CCWGG 1 cut(s) 444
BstSCI CCNGG 3 cut(s) 48, 226, 442
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 155
BstV2I GAAGAC 1 cut(s) 209
BstXI CCANNNNNNTGG 2 cut(s) 403, 470
BstZI CGGCCG 1 cut(s) 234
BsuRI GGCC 3 cut(s) 104, 236, 343
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 188, 218
BtsIMutI CAGTG 2 cut(s) 120, 470
Cfr10I RCCGGY 4 cut(s) 152, 172, 232, 236
Cfr13I GGNCC 3 cut(s) 230, 341, 446
CpoI CGGWCCG 1 cut(s) 230
CseI GACGC 1 cut(s) 178
Csp6I GTAC 1 cut(s) 304
CspI CGGWCCG 1 cut(s) 230
CviQI GTAC 1 cut(s) 304
DpnI GATC 3 cut(s) 20, 62, 70
DpnII GATC 3 cut(s) 18, 60, 68
EaeI YGGCCR 1 cut(s) 234
EagI CGGCCG 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EclXI CGGCCG 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 230, 446
Eco52I CGGCCG 1 cut(s) 234
EcoRII CCWGG 1 cut(s) 442
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 131
FaiI YATR 1 cut(s) 149
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 2 cut(s) 195, 225
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 2 cut(s) 392, 456
GluI GCNGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 3 cut(s) 104, 236, 343
HapII CCGG 7 cut(s) 49, 64, 153, 173, 228, 233, 237
HgaI GACGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 170
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HpaII CCGG 7 cut(s) 49, 64, 153, 173, 228, 233, 237
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 3 cut(s) 168, 306, 449
Hpy188I TCNGA 2 cut(s) 18, 248
Hpy188III TCNNGA 1 cut(s) 134
Hpy8I GTNNAC 3 cut(s) 168, 306, 449
Hpy99I CGWCG 2 cut(s) 43, 172
HpyAV CCTTC 1 cut(s) 251
HpyCH4III ACNGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 291, 340
Hsp92I GRCGYC 1 cut(s) 170
Kzo9I GATC 3 cut(s) 18, 60, 68
LmnI GCTCC 1 cut(s) 248
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 1 cut(s) 294
MaeI CTAG 2 cut(s) 392, 456
MaeIII GTNAC 2 cut(s) 186, 458
MalI GATC 3 cut(s) 20, 62, 70
MboI GATC 3 cut(s) 18, 60, 68
MboII GAAGA 2 cut(s) 23, 214
MluCI AATT 2 cut(s) 55, 72
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 3 cut(s) 37, 234, 361
MseI TTAA 3 cut(s) 75, 242, 475
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 7 cut(s) 49, 64, 153, 173, 228, 233, 237
MspR9I CCNGG 3 cut(s) 50, 228, 444
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 3 cut(s) 149, 291, 340
NciI CCSGG 2 cut(s) 50, 228
NdeII GATC 3 cut(s) 18, 60, 68
NlaIV GGNNCC 1 cut(s) 441
NmuCI GTSAC 2 cut(s) 186, 458
PfoI TCCNGGA 1 cut(s) 226
PkrI GCNGC 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 3 cut(s) 230, 341, 446
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 1 cut(s) 401
Rsr2I CGGWCCG 1 cut(s) 230
RsrII CGGWCCG 1 cut(s) 230
SalI GTCGAC 1 cut(s) 166
SaqAI TTAA 3 cut(s) 75, 242, 475
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 3 cut(s) 18, 60, 68
Sau96I GGNCC 3 cut(s) 230, 341, 446
ScrFI CCNGG 3 cut(s) 50, 228, 444
SetI ASST 7 cut(s) 88, 148, 226, 262, 269, 331, 448
SfaNI GCATC 1 cut(s) 294
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 2 cut(s) 230, 446
SmiMI CAYNNNNRTG 1 cut(s) 401
Sse9I AATT 2 cut(s) 55, 72
SsiI CCGC 1 cut(s) 362
SspMI CTAG 2 cut(s) 392, 456
StyD4I CCNGG 3 cut(s) 48, 226, 442
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 39
TaqI TCGA 2 cut(s) 167, 366
TasI AATT 2 cut(s) 55, 72
TatI WGTACW 1 cut(s) 303
Tru1I TTAA 3 cut(s) 75, 242, 475
Tru9I TTAA 3 cut(s) 75, 242, 475
TscAI CASTG 2 cut(s) 127, 470
TseFI GTSAC 2 cut(s) 186, 458
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 2 cut(s) 186, 458
TspRI CASTG 2 cut(s) 127, 470
VpaK11BI GGWCC 2 cut(s) 230, 446
XmiI GTMKAC 1 cut(s) 167
XspI CTAG 2 cut(s) 392, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.