Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Forward (+)

19706820 .. 19711460

Gene Structure 4641 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G53180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGAGTTTAGTGAGTTAAAGGAAGCAATAGAGAAGATAGAGCTCGTTGATGCTCACGCACACAACATTGTCTCTCTTGACTCATCTTTTCCGTTCATCGGAACTTTCTCCGAGGCTGCCGGCGACGCTTTAACCTTCGCTCCTCATTCTCTCTCCTTCAAGAGGAACTTGAGGGAGATTGCTCAACTTTATGGCACTGAAGTATCTTTAGAAGCGGTTGAGGAACATCGTAAAACCTCGGGTTTGGATTCTTTTACTTCCAAGTGTTTTAAAGAAGCTCGAATCTCTGCTCTCCTCATTGATGATGGATTAAAGCTAGACAAGAAACATGATATTGAATGGCATAGAAACTTTGTTCCATTCGTTGGTAGAGTGTTGCGTATCGAAACTCTTGCCGAGCAAATCCTCGAAGAGGAGTGTCCTGGTGGTTATTTCTATGGTTCTGAGAGTACTGAACCGCCTGTTTGGGATTTAGATTCTTTCACCAAAACCTTTGTTGAAAGATTAAATTCGCTTGTTCCAGAGATTGTTGCTTTGAAAACTATTGCTGCATATCGTAGCGGTTTGGATATTGATACTTATGTGAGTAAAGAAGTTGCTGAGAATGGCCTTGTTGAAGTCTTGCGAGCTGGAAAGCCTGTCCGCATAGGAAACAAGGGCTTGATTGACTACATCCTTACCATTAGTTTGGAGGTCGCTGTGCGTCGCGACTTGCCCTTGCAGATTCACACCGGTTTTGGTGACAAGGATTTGGATTTGAGGTTGTCGAATCCTCTTCACCTTAGAACCCTTCTTGAAGACAAAAGATTTGGAAAGTGTCGTATAGTTCTTCTTCATGCAGCTTATCCATTCTCAAAGGAAGCATCCTTTCTGTCTTCAGTTTATCCTCAGGTTTACCTTGATTTCGGCTTGGCGGTTCCAAAGCTTAGTGTCCATGGTATGGTTTCTTCAGTTAAAGAGCTCCTTGACTTAGCATCAATAAAGAAGGTTATGTTCAGCACTGATGGGTATGCATCTCCTGAGACCTACTATTTAGGTGCAAAGAAAGCACGTGAGGTTATCTTCTTAGTTCTGAGTGATGCTTGTGCCAGTGGTGATCTCTCACTCATGGAAGCTATTGATGCAGCTAAAGACATATTCTCACGAAATTCTATTGGATTTTATAAGCTTAATATAGACACAGATTCTTCTAGTCCACAAAATATAATATCTCCCAAATTGAAGATAAAGGAGCCTGATGTTCAAGAAGATAGTAGTTCTTTTGTACGCATTATTTGGGTTGATACATCTGGACAACAAAGATGCCGTGCTGTTCAAGCGCAGCGTTTTAACAGAAGTGTGAAGAAGAACGGCGTTGGTCTGACCTTTGCATCAATGGGGATGACTTCATTTACTGATGGACCAGCAGAAGAGTCTAAATTGACAGGTGTGGGTGAGATTAGACTTGTTCCAGATCTATCAACCAAGCAGACAATACCTTGGACGAAGCAAGAGAGCATGGTTTTAGCTGATATGCAATTGAAGCCTGGTGAAGCATGGGGGTACTGTCCACGAGAGACCTTAAGAAGGGTCGCAAAAGTTCTCAAAGATGAATTTGACTTGGTAATGAACGCTGGTTTTGAGAATGAGTTTTATCTCCTCAAGAACGTTGTGCGGGAAGGGAAAGAAGAGTATATGCCTTTTGACTTTGGTCCTTACTGTGCCACATCCTCATTCGATGCTGCATCTCCGATCTTCCATGATATTGTACCCGCACTCGAGTCCTTGAACATCGAAGTTGAACAGTTTCATGCTGAATCCGGGAAAGGTCAGTTTGAAGTATCTCTCGGTCACACCATTGCATCCCATGCTGCCGACAACTTGGTCTACACACGTGAAGTTATAAGATCGGTTGCAAGGAAACAGGGATTGCTCGCAACCTTTGTTCCAAAGTATGATTATTGTGACATTGGTTCTGGATCACATGTGCATCTAAGTCTTTGGAAAAACGGCGAAAATGTCTTCCCGGCATCTAATAACTCATCTTCTCATGGAATCTCTTCCGTTGGAGAAGAGTTCATGGCCGGAGTTCTATTTCATCTTCCATCAATCTTAGCTATTATAGCTCCACTCCCAAACAGCTACGACCGAATCCAACCTAATACATGGAGTGGAGCATTCCAATGTTGGGGCAAAGAAAACCGCGAAGCAGCGTTAAGAGCAGCTTCTCCACCAGGAACTCCTGATGGTTTAGTTACTAACTTTGAGATAAAGTCTTTTGATGGCTCTGCAAATCCTCACCTTGGTTTGGCTGTTATAATGGCTGCTGGTATTGATGGTCTCCGGCGACATCTTCAACTTCCTACTCCAATAGATATAAATCCGGCGGATGTGGCTGCTACATTGAACAGACTTCCGGAAACGCTCTCAGAAGCTGTTGAAGCTCTGGACAAAGACAAAGTTCTCCACGATTTGCTCGGACAAAAGCTTTTAGTTGCCATAAAAGGAGTCCGTAAGGCTGAGGTCGAGTATTATTCGAAGAATCCGGATGCATATAAGCAACTCATTCACCGATACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

852

Amino Acids

94.38

Weight (kDa)

5.56

Isoelectric Point (pI)

41.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_2	PF04909	230 - 357	8.1e-14	Amidohydrolase
Gln-synt_N_2	PF16952	418 - 495	5.3e-06	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	515 - 845	4.5e-97	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1164, 1883, 2297
AasI	GACNNNNNNGTC	1 cut(s)	1862
AccB7I	CCANNNNNTGG	2 cut(s)	365, 940
AccI	GTMKAC	1 cut(s)	1866
AccII	CGCG	2 cut(s)	708, 2184
AccIII	TCCGGA	2 cut(s)	2395, 2524
AciI	CCGC	9 cut(s)	215, 458, 561, 643, 914, 1654, 1752, 2182, 2366
AclI	AACGTT	1 cut(s)	1647
AclWI	GGATC	1 cut(s)	1966
AcoI	YGGCCR	1 cut(s)	2061
AcsI	RAATTY	3 cut(s)	508, 1147, 1592
AcuI	CTGAAG	3 cut(s)	219, 861, 933
AcvI	CACGTG	2 cut(s)	1052, 1874
AfaI	GTAC	4 cut(s)	451, 1266, 1544, 1749
AfiI	CCNNNNNNNGG	9 cut(s)	98, 162, 365, 412, 940, 1566, 2167, 2282, 2287
AflII	CTTAAG	1 cut(s)	1561
AflIII	ACRYGT	2 cut(s)	1871, 1963
AgeI	ACCGGT	1 cut(s)	731
AjnI	CCWGG	3 cut(s)	421, 1525, 2212
Alw21I	GWGCWC	2 cut(s)	45, 963
Alw26I	GTCTC	4 cut(s)	76, 1016, 1550, 2324
AlwI	GGATC	1 cut(s)	1966
AlwNI	CAGNNNCTG	1 cut(s)	2414
Ama87I	CYCGRG	2 cut(s)	238, 1757
Aor13HI	TCCGGA	2 cut(s)	2395, 2524
AoxI	GGCC	2 cut(s)	607, 2061
ApoI	RAATTY	3 cut(s)	508, 1147, 1592
ArsI	GACNNNNNNTTYG	2 cut(s)	791, 823
AsiGI	ACCGGT	1 cut(s)	731
Asp700I	GAANNNNTTC	2 cut(s)	1785, 2038
AspLEI	GCGC	1 cut(s)	1321
AspS9I	GGNCC	2 cut(s)	1400, 1691
AsuC2I	CCSGG	2 cut(s)	1801, 2006
AsuHPI	GGTGA	8 cut(s)	475, 752, 770, 1106, 1445, 1541, 2270, 2540
AsuII	TTCGAA	1 cut(s)	2516
AvaI	CYCGRG	2 cut(s)	238, 1757
AvaII	GGWCC	2 cut(s)	1400, 1691
AxyI	CCTNAGG	1 cut(s)	888
BaeI	ACNNNNGTAYC	4 cut(s)	186, 219, 567, 600
BanII	GRGCYC	2 cut(s)	45, 963
BarI	GAAGNNNNNNTAC	2 cut(s)	931, 963
BauI	CACGAG	1 cut(s)	1551
BbrPI	CACGTG	2 cut(s)	1052, 1874
BbsI	GAAGAC	3 cut(s)	804, 867, 1993
Bbv12I	GWGCWC	2 cut(s)	45, 963
BbvCI	CCTCAGC	1 cut(s)	2499
BccI	CCATC	7 cut(s)	299, 998, 1391, 2092, 2219, 2255, 2309
BceAI	ACGGC	3 cut(s)	1290, 1366, 2005
BcgI	CGANNNNNNTGC	2 cut(s)	374, 408
BciT130I	CCWGG	3 cut(s)	423, 1527, 2214
BcnI	CCSGG	2 cut(s)	1801, 2006
BcoDI	GTCTC	4 cut(s)	76, 1016, 1550, 2324
BfaI	CTAG	2 cut(s)	317, 1191
BfrI	CTTAAG	1 cut(s)	1561
BglII	AGATCT	1 cut(s)	1453
BmcAI	AGTACT	1 cut(s)	451
Bme1390I	CCNGG	5 cut(s)	423, 1527, 1801, 2006, 2214
Bme18I	GGWCC	2 cut(s)	1400, 1691
BmeT110I	CYCGRG	2 cut(s)	238, 1757
BmgT120I	GGNCC	2 cut(s)	1400, 1691
BmiI	GGNNCC	2 cut(s)	918, 1233
BmrFI	CCNGG	5 cut(s)	423, 1527, 1801, 2006, 2214
BoxI	GACNNNNGTC	1 cut(s)	1689
BpiI	GAAGAC	3 cut(s)	804, 867, 1993
Bpu10I	CCTNAGC	1 cut(s)	2499
Bpu14I	TTCGAA	1 cut(s)	2516
BpuEI	CTTGAG	2 cut(s)	190, 1625
BpuMI	CCSGG	2 cut(s)	1801, 2006
BsaAI	YACGTR	2 cut(s)	1052, 1874
BsaBI	GATNNNNATC	1 cut(s)	2358
BsaI	GGTCTC	3 cut(s)	1016, 1550, 2324
BsaJI	CCNNGG	5 cut(s)	111, 237, 934, 1478, 2281
BsaWI	WCCGGW	3 cut(s)	731, 2395, 2524
BsaXI	ACNNNNNCTCC	2 cut(s)	124, 154
Bsc4I	CCNNNNNNNGG	9 cut(s)	98, 162, 365, 412, 940, 1566, 2167, 2282, 2287
Bse118I	RCCGGY	2 cut(s)	119, 731
Bse1I	ACTGG	1 cut(s)	1089
Bse21I	CCTNAGG	1 cut(s)	888
Bse3DI	GCAATG	1 cut(s)	1836
Bse8I	GATNNNNATC	1 cut(s)	2358
BseAI	TCCGGA	2 cut(s)	2395, 2524
BseBI	CCWGG	3 cut(s)	423, 1527, 2214
BseDI	CCNNGG	5 cut(s)	111, 237, 934, 1478, 2281
BseGI	GGATG	7 cut(s)	672, 863, 1386, 1706, 1841, 2374, 2533
BseJI	GATNNNNATC	1 cut(s)	2358
BseLI	CCNNNNNNNGG	9 cut(s)	98, 162, 365, 412, 940, 1566, 2167, 2282, 2287
BseMI	GCAATG	1 cut(s)	1836
BseMII	CTCAG	7 cut(s)	435, 591, 902, 1011, 1064, 2421, 2490
BseNI	ACTGG	1 cut(s)	1089
BseRI	GAGGAG	4 cut(s)	132, 284, 428, 1628
Bsh1236I	CGCG	2 cut(s)	708, 2184
Bsh1285I	CGRYCG	1 cut(s)	2128
BshFI	GGCC	2 cut(s)	609, 2063
BshTI	ACCGGT	1 cut(s)	731
BsiEI	CGRYCG	1 cut(s)	2128
BsiHKAI	GWGCWC	2 cut(s)	45, 963
BsiHKCI	CYCGRG	2 cut(s)	238, 1757
BsiSI	CCGG	9 cut(s)	120, 732, 1800, 2006, 2064, 2323, 2363, 2396, 2525
BslI	CCNNNNNNNGG	9 cut(s)	98, 162, 365, 412, 940, 1566, 2167, 2282, 2287
BsmAI	GTCTC	4 cut(s)	76, 1016, 1550, 2324
BsmI	GAATGC	1 cut(s)	2156
BsnI	GGCC	2 cut(s)	609, 2063
Bso31I	GGTCTC	3 cut(s)	1016, 1550, 2324
BsoBI	CYCGRG	2 cut(s)	238, 1757
Bsp119I	TTCGAA	1 cut(s)	2516
Bsp1286I	GDGCHC	2 cut(s)	45, 963
Bsp13I	TCCGGA	2 cut(s)	2395, 2524
Bsp143I	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
Bsp19I	CCATGG	1 cut(s)	934
Bsp68I	TCGCGA	1 cut(s)	708
BspACI	CCGC	9 cut(s)	215, 458, 561, 643, 914, 1654, 1752, 2182, 2366
BspANI	GGCC	2 cut(s)	609, 2063
BspCNI	CTCAG	7 cut(s)	436, 592, 901, 1012, 1065, 2420, 2491
BspEI	TCCGGA	2 cut(s)	2395, 2524
BspFNI	CGCG	2 cut(s)	708, 2184
BspLI	GGNNCC	2 cut(s)	918, 1233
BspPI	GGATC	1 cut(s)	1966
BspT104I	TTCGAA	1 cut(s)	2516
BspTI	CTTAAG	1 cut(s)	1561
BspTNI	GGTCTC	3 cut(s)	1016, 1550, 2324
BsrDI	GCAATG	1 cut(s)	1836
BsrFI	RCCGGY	2 cut(s)	119, 731
BsrI	ACTGG	1 cut(s)	1089
BssAI	RCCGGY	2 cut(s)	119, 731
BssECI	CCNNGG	5 cut(s)	111, 237, 934, 1478, 2281
BssMI	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
BssSI	CACGAG	1 cut(s)	1551
BssT1I	CCWWGG	3 cut(s)	934, 1478, 2281
Bst2BI	CACGAG	1 cut(s)	1551
Bst2UI	CCWGG	3 cut(s)	423, 1527, 2214
Bst4CI	ACNGT	3 cut(s)	1547, 1700, 1785
Bst6I	CTCTTC	6 cut(s)	405, 780, 1404, 1662, 2044, 2046
BstAFI	CTTAAG	1 cut(s)	1561
BstAPI	GCANNNNNTGC	1 cut(s)	1847
BstBAI	YACGTR	2 cut(s)	1052, 1874
BstBI	TTCGAA	1 cut(s)	2516
BstC8I	GCNNGC	3 cut(s)	121, 627, 1914
BstDSI	CCRYGG	1 cut(s)	934
BstENI	CCTNNNNNAGG	3 cut(s)	160, 410, 1564
BstF5I	GGATG	7 cut(s)	672, 863, 1386, 1706, 1841, 2374, 2533
BstFNI	CGCG	2 cut(s)	708, 2184
BstHHI	GCGC	1 cut(s)	1321
BstKTI	GATC	5 cut(s)	1099, 1456, 1734, 1889, 1961
BstMAI	GTCTC	4 cut(s)	76, 1016, 1550, 2324
BstMBI	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
BstMCI	CGRYCG	1 cut(s)	2128
BstNI	CCWGG	3 cut(s)	423, 1527, 2214
BstNSI	RCATGY	1 cut(s)	1967
BstPAI	GACNNNNGTC	1 cut(s)	1689
BstSCI	CCNGG	5 cut(s)	421, 1525, 1799, 2004, 2212
BstUI	CGCG	2 cut(s)	708, 2184
BstV2I	GAAGAC	3 cut(s)	804, 867, 1993
BstX2I	RGATCY	1 cut(s)	1453
BstXI	CCANNNNNNTGG	1 cut(s)	688
BstYI	RGATCY	1 cut(s)	1453
Bsu36I	CCTNAGG	1 cut(s)	888
BsuRI	GGCC	2 cut(s)	609, 2063
BtgI	CCRYGG	1 cut(s)	934
BtsCI	GGATG	7 cut(s)	672, 863, 1386, 1706, 1841, 2374, 2533
BtsIMutI	CAGTG	3 cut(s)	195, 999, 1096
BtuMI	TCGCGA	1 cut(s)	708
Cac8I	GCNNGC	3 cut(s)	121, 627, 1914
CaiI	CAGNNNCTG	1 cut(s)	2414
CfoI	GCGC	1 cut(s)	1321
Cfr10I	RCCGGY	2 cut(s)	119, 731
Cfr13I	GGNCC	2 cut(s)	1400, 1691
CseI	GACGC	2 cut(s)	134, 692
Csp6I	GTAC	4 cut(s)	450, 1265, 1543, 1748
CspAI	ACCGGT	1 cut(s)	731
CviQI	GTAC	4 cut(s)	450, 1265, 1543, 1748
DpnI	GATC	5 cut(s)	1098, 1455, 1733, 1888, 1960
DpnII	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
DraI	TTTAAA	1 cut(s)	271
DrdI	GACNNNNNNGTC	1 cut(s)	1862
DseDI	GACNNNNNNGTC	1 cut(s)	1862
EaeI	YGGCCR	1 cut(s)	2061
Eam1104I	CTCTTC	6 cut(s)	405, 780, 1404, 1662, 2044, 2046
EarI	CTCTTC	6 cut(s)	405, 780, 1404, 1662, 2044, 2046
EciI	GGCGGA	1 cut(s)	2381
Ecl136II	GAGCTC	2 cut(s)	43, 961
Eco130I	CCWWGG	3 cut(s)	934, 1478, 2281
Eco24I	GRGCYC	2 cut(s)	45, 963
Eco31I	GGTCTC	3 cut(s)	1016, 1550, 2324
Eco47I	GGWCC	2 cut(s)	1400, 1691
Eco53kI	GAGCTC	2 cut(s)	43, 961
Eco57I	CTGAAG	3 cut(s)	219, 861, 933
Eco72I	CACGTG	2 cut(s)	1052, 1874
Eco81I	CCTNAGG	1 cut(s)	888
Eco88I	CYCGRG	2 cut(s)	238, 1757
EcoICRI	GAGCTC	2 cut(s)	43, 961
EcoNI	CCTNNNNNAGG	3 cut(s)	160, 410, 1564
EcoRII	CCWGG	3 cut(s)	421, 1525, 2212
EcoT14I	CCWWGG	3 cut(s)	934, 1478, 2281
EcoT22I	ATGCAT	2 cut(s)	1015, 2533
EcoT38I	GRGCYC	2 cut(s)	45, 963
ErhI	CCWWGG	3 cut(s)	934, 1478, 2281
FalI	AAGNNNNNCTT	6 cut(s)	152, 184, 948, 980, 2188, 2220
FauI	CCCGC	2 cut(s)	1647, 1759
FblI	GTMKAC	1 cut(s)	1866
FokI	GGATG	7 cut(s)	659, 850, 1393, 1693, 1828, 2381, 2540
FriOI	GRGCYC	2 cut(s)	45, 963
FspBI	CTAG	2 cut(s)	317, 1191
GlaI	GCGC	1 cut(s)	1320
HaeIII	GGCC	2 cut(s)	609, 2063
HapII	CCGG	9 cut(s)	120, 732, 1800, 2006, 2064, 2323, 2363, 2396, 2525
HgaI	GACGC	2 cut(s)	134, 692
HhaI	GCGC	1 cut(s)	1321
Hin6I	GCGC	1 cut(s)	1319
HinP1I	GCGC	1 cut(s)	1319
HindIII	AAGCTT	3 cut(s)	923, 1166, 2465
HpaII	CCGG	9 cut(s)	120, 732, 1800, 2006, 2064, 2323, 2363, 2396, 2525
HphI	GGTGA	8 cut(s)	475, 752, 770, 1106, 1445, 1541, 2270, 2540
Hpy166II	GTNNAC	4 cut(s)	895, 1196, 1550, 1867
Hpy188I	TCNGA	8 cut(s)	101, 112, 445, 1074, 1362, 1731, 2410, 2459
Hpy8I	GTNNAC	4 cut(s)	895, 1196, 1550, 1867
Hpy99I	CGWCG	2 cut(s)	128, 708
HpyAV	CCTTC	6 cut(s)	145, 166, 800, 979, 1560, 1652
HpyCH4III	ACNGT	3 cut(s)	1547, 1700, 1785
HpyCH4IV	ACGT	3 cut(s)	1051, 1647, 1873
HpySE526I	ACGT	3 cut(s)	1051, 1647, 1873
HspAI	GCGC	1 cut(s)	1319
Kpn2I	TCCGGA	2 cut(s)	2395, 2524
KroI	GCCGGC	1 cut(s)	119
KroNI	GCCGGC	1 cut(s)	121
Kzo9I	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
LmnI	GCTCC	5 cut(s)	145, 966, 1231, 2110, 2153
MaeI	CTAG	2 cut(s)	317, 1191
MaeII	ACGT	3 cut(s)	1051, 1647, 1873
MaeIII	GTNAC	4 cut(s)	740, 1829, 1943, 2233
MalI	GATC	5 cut(s)	1098, 1455, 1733, 1888, 1960
MboI	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
MfeI	CAATTG	1 cut(s)	1517
MflI	RGATCY	1 cut(s)	1453
MhlI	GDGCHC	2 cut(s)	45, 963
MluCI	AATT	6 cut(s)	508, 1147, 1217, 1418, 1517, 1592
MlyI	GAGTC	4 cut(s)	74, 1421, 1769, 2496
MmeI	TCCRAC	2 cut(s)	2026, 2158
Mph1103I	ATGCAT	2 cut(s)	1015, 2533
MroI	TCCGGA	2 cut(s)	2395, 2524
MroNI	GCCGGC	1 cut(s)	119
MroXI	GAANNNNTTC	2 cut(s)	1785, 2038
MslI	CAYNNNNRTG	1 cut(s)	2161
MspCI	CTTAAG	1 cut(s)	1561
MspI	CCGG	9 cut(s)	120, 732, 1800, 2006, 2064, 2323, 2363, 2396, 2525
MspR9I	CCNGG	5 cut(s)	423, 1527, 1801, 2006, 2214
MunI	CAATTG	1 cut(s)	1517
Mva1269I	GAATGC	1 cut(s)	2156
MvaI	CCWGG	3 cut(s)	423, 1527, 2214
MvnI	CGCG	2 cut(s)	708, 2184
NaeI	GCCGGC	1 cut(s)	121
NciI	CCSGG	2 cut(s)	1801, 2006
NcoI	CCATGG	1 cut(s)	934
NdeII	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
NgoMIV	GCCGGC	1 cut(s)	119
NlaIV	GGNNCC	2 cut(s)	918, 1233
NmeAIII	GCCGAG	1 cut(s)	421
NmuCI	GTSAC	3 cut(s)	740, 1829, 1943
NruI	TCGCGA	1 cut(s)	708
NsiI	ATGCAT	2 cut(s)	1015, 2533
NspI	RCATGY	1 cut(s)	1967
NspV	TTCGAA	1 cut(s)	2516
PaeR7I	CTCGAG	1 cut(s)	1757
PciI	ACATGT	1 cut(s)	1963
PctI	GAATGC	1 cut(s)	2156
PdiI	GCCGGC	1 cut(s)	121
PdmI	GAANNNNTTC	2 cut(s)	1785, 2038
PflMI	CCANNNNNTGG	2 cut(s)	365, 940
PfoI	TCCNGGA	1 cut(s)	1799
PinAI	ACCGGT	1 cut(s)	731
PleI	GAGTC	4 cut(s)	74, 1420, 1768, 2495
PmaCI	CACGTG	2 cut(s)	1052, 1874
PmlI	CACGTG	2 cut(s)	1052, 1874
PpsI	GAGTC	4 cut(s)	74, 1420, 1768, 2495
Ppu21I	YACGTR	2 cut(s)	1052, 1874
PscI	ACATGT	1 cut(s)	1963
PshAI	GACNNNNGTC	1 cut(s)	1689
PsiI	TTATAA	3 cut(s)	1164, 1883, 2297
Psp124BI	GAGCTC	2 cut(s)	45, 963
Psp1406I	AACGTT	1 cut(s)	1647
Psp6I	CCWGG	3 cut(s)	421, 1525, 2212
PspCI	CACGTG	2 cut(s)	1052, 1874
PspGI	CCWGG	3 cut(s)	421, 1525, 2212
PspN4I	GGNNCC	2 cut(s)	918, 1233
PspPI	GGNCC	2 cut(s)	1400, 1691
PspXI	VCTCGAGB	1 cut(s)	1757
PstNI	CAGNNNCTG	1 cut(s)	2414
PsuI	RGATCY	1 cut(s)	1453
RruI	TCGCGA	1 cut(s)	708
RsaI	GTAC	4 cut(s)	451, 1266, 1544, 1749
RsaNI	GTAC	4 cut(s)	450, 1265, 1543, 1748
RseI	CAYNNNNRTG	1 cut(s)	2161
SacI	GAGCTC	2 cut(s)	45, 963
Sau3AI	GATC	5 cut(s)	1096, 1453, 1731, 1886, 1958
Sau96I	GGNCC	2 cut(s)	1400, 1691
ScaI	AGTACT	1 cut(s)	451
SchI	GAGTC	4 cut(s)	74, 1421, 1769, 2496
ScrFI	CCNGG	5 cut(s)	423, 1527, 1801, 2006, 2214
SduI	GDGCHC	2 cut(s)	45, 963
Sfr274I	CTCGAG	1 cut(s)	1757
SfuI	TTCGAA	1 cut(s)	2516
SinI	GGWCC	2 cut(s)	1400, 1691
SlaI	CTCGAG	1 cut(s)	1757
SmiMI	CAYNNNNRTG	1 cut(s)	2161
SmlI	CTYRAG	4 cut(s)	169, 1561, 1640, 1757
SmoI	CTYRAG	4 cut(s)	169, 1561, 1640, 1757
Sse9I	AATT	6 cut(s)	508, 1147, 1217, 1418, 1517, 1592
SsiI	CCGC	9 cut(s)	215, 458, 561, 643, 914, 1654, 1752, 2182, 2366
SspMI	CTAG	2 cut(s)	317, 1191
SstI	GAGCTC	2 cut(s)	45, 963
StyD4I	CCNGG	5 cut(s)	421, 1525, 1799, 2004, 2212
StyI	CCWWGG	3 cut(s)	934, 1478, 2281
TaaI	ACNGT	3 cut(s)	1547, 1700, 1785
TaiI	ACGT	3 cut(s)	1054, 1650, 1876
TaqI	TCGA	9 cut(s)	280, 384, 408, 767, 1716, 1758, 1773, 2505, 2516
TaqII	GACCGA	2 cut(s)	1817, 2142
TasI	AATT	6 cut(s)	508, 1147, 1217, 1418, 1517, 1592
TatI	WGTACW	1 cut(s)	449
TscAI	CASTG	3 cut(s)	202, 1006, 1096
TseFI	GTSAC	3 cut(s)	740, 1829, 1943
Tsp45I	GTSAC	3 cut(s)	740, 1829, 1943
TspDTI	ATGAA	8 cut(s)	85, 824, 1377, 1605, 1622, 1778, 2047, 2066
TspGWI	ACGGA	3 cut(s)	81, 2032, 2480
TspRI	CASTG	3 cut(s)	202, 1006, 1096
Van91I	CCANNNNNTGG	2 cut(s)	365, 940
Vha464I	CTTAAG	1 cut(s)	1561
VpaK11BI	GGWCC	2 cut(s)	1400, 1691
XagI	CCTNNNNNAGG	3 cut(s)	160, 410, 1564
XapI	RAATTY	3 cut(s)	508, 1147, 1592
XceI	RCATGY	1 cut(s)	1967
XhoI	CTCGAG	1 cut(s)	1757
XmiI	GTMKAC	1 cut(s)	1866
XmnI	GAANNNNTTC	2 cut(s)	1785, 2038
XspI	CTAG	2 cut(s)	317, 1191
ZrmI	AGTACT	1 cut(s)	451
Zsp2I	ATGCAT	2 cut(s)	1015, 2533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.