Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Reverse (-)

13743741 .. 13751062

Gene Structure 7322 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG134300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGGATTTCACGGGGCTGCGGGCGGCGGTGGAGGAGGCGGAGCTAGTCGACGCCCACGCCCACAACATCGTCGCCACCGACTCCTCCTTCCCCTTCATCAGTGCCTTCTCCGAAGCCAACGCCGATGGCTTATCCTACGCACCCCATTCCCTCTCCTTCAAGAGAAGTTTGAAGGAGGTTGCTGAGCTGTACGGCTGTGACAAGTGCTTGGATGCGGTTGAGGAGCATCGGAGAGTCGCCGGATTGGAGGCCATTAGCTCCAGATGCTTTGAGGCTGCGAGAATCACTGCGTTACTTATTGATGATGGACTGAGGCTGGACAAAATGCATGACATAGATTGGCATAGGAGTTTCGCTCCTGTGGTTGGGAGAATTCTGAGGATTGAGTCTCTGGCTGAGCAGATTCTTAGTGAGGTCATGTTCAGCACTGATGGATATGCATTTCCTGAAACTTTCTACTTAGGTGCAAAAAAGGCACGTGACGTTGTCTTTTCAGTTCTATGTGATGCCTGTGCCGATGGTGATCTCTCAATTCCGGAAGCTATTGAGGCTGCTAAAGACATCTTTTCACAAAATGCAGTTCAGTTGTACAAGATTGATAGTGCTGTCAAATCTTCTGGTTCAGCCAATAGTGTATCCTCTAACTTTGTGAAGGTGAAGAGTAATGACTCGGAAAATCATGTCTCACTTGTTCGTGTTTTCTGGGCTGATGCATCAGGACAACAAAGATGTCGTGTTGTTCCTGCTAAGCGGTTCAATGACATCGTTACAAAAAATGGTATTGGTTTGACTTTTGCTTGTATGGGAGTGCCTTCTTTCACTGACGGTCCAGCTGATGAGACTAACTTGACTGGAGTGGGTGAGATCAGACTCGTGCCTGATTTATCGACTAAACGGAGAATTCCATGGGTAGAACAAGAGGAAATGGTTTTGGCCAACATGCATCTTAAACCAGGTGAAGCATGGGAATACTGCCCAAGAGAGGCCTTACGGAGGGTTTCAAAAATATTGAAAGATGAATTCAATTTGGTAATGAATGCAGGATTTGAAAATGAATTTTTTCTCTTGAAGAGTATGCTAAGGGACGGGAAAGAAGAATGGGTACCATTTGACTCAACACCCTACTGTTCCCCATCATCATATGATGCTGCTTCCCCTTTGTTTCAAGAAGTTCTTGCTGCTCTCCAGTCCTTGAATATTACTGTAGAACAGTTACATGCAGAAAGTGGGAAAGGTCAATTTGAGGTGGTTCTAGGGCACACAGCTTGCCAGCATGCTGCTGACAACTTGATTTATACCCGTGAAGTTATTAGGGCCATTGCAAGGAAACACGGATTGTTGGCAACCTTTGTCCCAAAGTATGCGCTAGATGAGATTGGCTCTGGAGCCCATGTACATCTCAGTTTGTGGCAGAATGGCACAAATGTATTCATGGCATCTGGTGGATCCTCTCAGCATGGAATGTCCAAGGTTGGGGAGGAATTTATGGCAGGGGTTTTATATCATCTCCCAGCAATTTTGGCATTTACAGCTCCAATTCCAAATAGTTACGACCGGATACAACCTAATACTTGGAGTGGAGCATACAAGTGCTGGGGAAAAGAAAACAGGGAAGCTCCATTAAGAACTGCATGCCCACCTGGAGTTCAAGCTGGTCTGGTGAGCAACTTTGAGATCAAGTCATTTGATGGGTGTGCAAATCCACACTTGGGTTTGGCTGCAATACTAGCTGCTGGCATTGATGGTCTTAGGAGACATCTTTGTCTCCCTGAACCTATTGATACAAATCCTTCTAGCCTTGAAGGAGAAATACAAAGGTTACCAACTTCACTTTTGGAGTCATTAGAAGCATTAAAGGAAGATGATCTCTTCAAAGAGTTCATTGGAGAAAAGCTATTGGTTGCCATCAAGGGAGTCCGCAAGGCAGAATTTGACTACTACGAGAAGCACAAAGATGCATACAAGCAACTTATACACCGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

661

Amino Acids

72.9

Weight (kDa)

5.43

Isoelectric Point (pI)

47.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_N_2	PF16952	218 - 303	2e-06	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	324 - 653	9.6e-92	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1761
Acc65I	GGTACC	1 cut(s)	1102
AccB1I	GGYRCC	1 cut(s)	1102
AccI	GTMKAC	1 cut(s)	48
AccIII	TCCGGA	1 cut(s)	535
AciI	CCGC	8 cut(s)	19, 23, 26, 38, 215, 751, 1918, 1978
AclWI	GGATC	2 cut(s)	1440, 1453
AcoI	YGGCCR	1 cut(s)	933
AcsI	RAATTY	6 cut(s)	372, 900, 1019, 1055, 1481, 1928
AcvI	CACGTG	1 cut(s)	479
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	4 cut(s)	191, 590, 1104, 1395
AfiI	CCNNNNNNNGG	6 cut(s)	365, 982, 993, 1323, 1473, 1709
AjnI	CCWGG	2 cut(s)	952, 1639
Alw26I	GTCTC	5 cut(s)	393, 688, 833, 1747, 1769
AlwI	GGATC	2 cut(s)	1440, 1453
Aor13HI	TCCGGA	1 cut(s)	535
AoxI	GGCC	4 cut(s)	249, 933, 984, 1314
ApeKI	GCWGC	8 cut(s)	16, 275, 551, 1148, 1178, 1277, 1718, 1730
ApoI	RAATTY	6 cut(s)	372, 900, 1019, 1055, 1481, 1928
Asp700I	GAANNNNTTC	1 cut(s)	1059
Asp718I	GGTACC	1 cut(s)	1102
AspLEI	GCGC	1 cut(s)	1366
AspS9I	GGNCC	2 cut(s)	827, 1314
AsuHPI	GGTGA	5 cut(s)	533, 667, 872, 968, 1672
AvaII	GGWCC	1 cut(s)	827
BaeGI	GKGCMC	1 cut(s)	1260
BalI	TGGCCA	1 cut(s)	935
BamHI	GGATCC	1 cut(s)	1445
BanI	GGYRCC	1 cut(s)	1102
BanII	GRGCYC	1 cut(s)	1390
BarI	GAAGNNNNNNTAC	4 cut(s)	1086, 1118, 1794, 1826
BauI	CACGAG	1 cut(s)	872
BbrPI	CACGTG	1 cut(s)	479
BbvI	GCAGC	8 cut(s)	3, 262, 538, 1135, 1165, 1264, 1705, 1717
BccI	CCATC	8 cut(s)	119, 299, 425, 512, 1141, 1682, 1736, 1913
BceAI	ACGGC	1 cut(s)	208
BciT130I	CCWGG	2 cut(s)	954, 1641
BciVI	GTATCC	2 cut(s)	646, 1551
BcoDI	GTCTC	5 cut(s)	393, 688, 833, 1747, 1769
BfaI	CTAG	5 cut(s)	44, 1253, 1367, 1727, 1794
BfmI	CTRYAG	1 cut(s)	1203
BfuI	GTATCC	2 cut(s)	646, 1551
BisI	GCNGC	9 cut(s)	17, 24, 276, 552, 1149, 1179, 1278, 1719, 1731
BlpI	GCTNAGC	3 cut(s)	183, 396, 747
BlsI	GCNGC	9 cut(s)	18, 25, 277, 553, 1150, 1180, 1279, 1720, 1732
Bme1390I	CCNGG	2 cut(s)	954, 1641
Bme18I	GGWCC	1 cut(s)	827
BmgT120I	GGNCC	2 cut(s)	827, 1314
BmiI	GGNNCC	3 cut(s)	1104, 1387, 1447
BmrFI	CCNGG	2 cut(s)	954, 1641
BplI	GAGNNNNNCTC	4 cut(s)	340, 372, 1364, 1396
BpmI	CTGGAG	5 cut(s)	244, 873, 1169, 1404, 1662
Bpu10I	CCTNAGC	1 cut(s)	1079
Bpu1102I	GCTNAGC	3 cut(s)	183, 396, 747
BsaAI	YACGTR	1 cut(s)	479
BsaBI	GATNNNNATC	2 cut(s)	522, 1785
BsaHI	GRCGYC	1 cut(s)	51
BsaJI	CCNNGG	2 cut(s)	905, 1467
BsaWI	WCCGGW	2 cut(s)	535, 1554
BsaXI	ACNNNNNCTCC	2 cut(s)	1377, 1407
Bsc4I	CCNNNNNNNGG	6 cut(s)	365, 982, 993, 1323, 1473, 1709
Bse1I	ACTGG	2 cut(s)	856, 1186
Bse3DI	GCAATG	1 cut(s)	1317
Bse8I	GATNNNNATC	2 cut(s)	522, 1785
BseAI	TCCGGA	1 cut(s)	535
BseBI	CCWGG	2 cut(s)	954, 1641
BseDI	CCNNGG	2 cut(s)	905, 1467
BseGI	GGATG	1 cut(s)	217
BseJI	GATNNNNATC	2 cut(s)	522, 1785
BseLI	CCNNNNNNNGG	6 cut(s)	365, 982, 993, 1323, 1473, 1709
BseMI	GCAATG	1 cut(s)	1317
BseMII	CTCAG	6 cut(s)	174, 302, 368, 387, 1414, 1466
BseNI	ACTGG	2 cut(s)	856, 1186
BseRI	GAGGAG	3 cut(s)	47, 73, 236
BseSI	GKGCMC	1 cut(s)	1260
BseXI	GCAGC	8 cut(s)	3, 262, 538, 1135, 1165, 1264, 1705, 1717
BseYI	CCCAGC	2 cut(s)	1510, 1593
Bsh1285I	CGRYCG	1 cut(s)	1555
BshFI	GGCC	4 cut(s)	251, 935, 986, 1316
BshNI	GGYRCC	1 cut(s)	1102
BsiEI	CGRYCG	1 cut(s)	1555
BsiSI	CCGG	3 cut(s)	240, 536, 1555
BslFI	GGGAC	2 cut(s)	1097, 1337
BslI	CCNNNNNNNGG	6 cut(s)	365, 982, 993, 1323, 1473, 1709
BsmAI	GTCTC	5 cut(s)	393, 688, 833, 1747, 1769
BsmFI	GGGAC	2 cut(s)	1097, 1337
BsmI	GAATGC	1 cut(s)	1042
BsnI	GGCC	4 cut(s)	251, 935, 986, 1316
Bsp1286I	GDGCHC	2 cut(s)	1260, 1390
Bsp13I	TCCGGA	1 cut(s)	535
Bsp1407I	TGTACA	2 cut(s)	588, 1393
Bsp143I	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
Bsp1720I	GCTNAGC	3 cut(s)	183, 396, 747
Bsp19I	CCATGG	1 cut(s)	905
BspACI	CCGC	8 cut(s)	19, 23, 26, 38, 215, 751, 1918, 1978
BspANI	GGCC	4 cut(s)	251, 935, 986, 1316
BspCNI	CTCAG	6 cut(s)	175, 303, 369, 388, 1413, 1465
BspEI	TCCGGA	1 cut(s)	535
BspLI	GGNNCC	3 cut(s)	1104, 1387, 1447
BspPI	GGATC	2 cut(s)	1440, 1453
BspT107I	GGYRCC	1 cut(s)	1102
BsrDI	GCAATG	1 cut(s)	1317
BsrGI	TGTACA	2 cut(s)	588, 1393
BsrI	ACTGG	2 cut(s)	856, 1186
BssECI	CCNNGG	2 cut(s)	905, 1467
BssMI	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
BssNI	GRCGYC	1 cut(s)	51
BssSI	CACGAG	1 cut(s)	872
BssT1I	CCWWGG	2 cut(s)	905, 1467
Bst2BI	CACGAG	1 cut(s)	872
Bst2UI	CCWGG	2 cut(s)	954, 1641
Bst4CI	ACNGT	4 cut(s)	827, 1127, 1204, 1212
Bst6I	CTCTTC	3 cut(s)	653, 1064, 1874
BstACI	GRCGYC	1 cut(s)	51
BstAUI	TGTACA	2 cut(s)	588, 1393
BstBAI	YACGTR	1 cut(s)	479
BstC8I	GCNNGC	6 cut(s)	21, 1267, 1271, 1275, 1633, 1735
BstDSI	CCRYGG	1 cut(s)	905
BstEII	GGTNACC	1 cut(s)	1818
BstENI	CCTNNNNNAGG	1 cut(s)	991
BstF5I	GGATG	1 cut(s)	217
BstHHI	GCGC	1 cut(s)	1366
BstKTI	GATC	5 cut(s)	526, 867, 1448, 1677, 1867
BstMAI	GTCTC	5 cut(s)	393, 688, 833, 1747, 1769
BstMBI	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
BstMCI	CGRYCG	1 cut(s)	1555
BstMWI	GCNNNNNNNGC	6 cut(s)	264, 473, 548, 1520, 1529, 1727
BstNI	CCWGG	2 cut(s)	954, 1641
BstNSI	RCATGY	4 cut(s)	943, 1220, 1277, 1635
BstPI	GGTNACC	1 cut(s)	1818
BstSCI	CCNGG	2 cut(s)	952, 1639
BstSFI	CTRYAG	1 cut(s)	1203
BstSLI	GKGCMC	1 cut(s)	1260
BstV1I	GCAGC	8 cut(s)	3, 262, 538, 1135, 1165, 1264, 1705, 1717
BstX2I	RGATCY	1 cut(s)	1445
BstYI	RGATCY	1 cut(s)	1445
BsuI	GTATCC	2 cut(s)	646, 1551
BsuRI	GGCC	4 cut(s)	251, 935, 986, 1316
BtgI	CCRYGG	1 cut(s)	905
BtsCI	GGATG	1 cut(s)	217
BtsI	GCAGTG	1 cut(s)	285
BtsIMutI	CAGTG	4 cut(s)	106, 285, 426, 819
Cac8I	GCNNGC	6 cut(s)	21, 1267, 1271, 1275, 1633, 1735
CfoI	GCGC	1 cut(s)	1366
Cfr13I	GGNCC	2 cut(s)	827, 1314
CseI	GACGC	1 cut(s)	59
CsiI	ACCWGGT	1 cut(s)	952
Csp6I	GTAC	4 cut(s)	190, 589, 1103, 1394
CviQI	GTAC	4 cut(s)	190, 589, 1103, 1394
DpnI	GATC	5 cut(s)	525, 866, 1447, 1676, 1866
DpnII	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
DrdI	GACNNNNNNGTC	1 cut(s)	1761
DseDI	GACNNNNNNGTC	1 cut(s)	1761
EaeI	YGGCCR	1 cut(s)	933
Eam1104I	CTCTTC	3 cut(s)	653, 1064, 1874
EarI	CTCTTC	3 cut(s)	653, 1064, 1874
EciI	GGCGGA	1 cut(s)	53
Eco130I	CCWWGG	2 cut(s)	905, 1467
Eco147I	AGGCCT	1 cut(s)	986
Eco24I	GRGCYC	1 cut(s)	1390
Eco47I	GGWCC	1 cut(s)	827
Eco72I	CACGTG	1 cut(s)	479
Eco91I	GGTNACC	1 cut(s)	1818
EcoNI	CCTNNNNNAGG	1 cut(s)	991
EcoO65I	GGTNACC	1 cut(s)	1818
EcoRI	GAATTC	3 cut(s)	372, 900, 1019
EcoRII	CCWGG	2 cut(s)	952, 1639
EcoT14I	CCWWGG	2 cut(s)	905, 1467
EcoT22I	ATGCAT	5 cut(s)	330, 442, 715, 945, 1960
EcoT38I	GRGCYC	1 cut(s)	1390
ErhI	CCWWGG	2 cut(s)	905, 1467
FalI	AAGNNNNNCTT	2 cut(s)	1158, 1190
FaqI	GGGAC	2 cut(s)	1097, 1337
FauI	CCCGC	1 cut(s)	12
FauNDI	CATATG	1 cut(s)	1141
FblI	GTMKAC	1 cut(s)	48
Fnu4HI	GCNGC	9 cut(s)	17, 24, 276, 552, 1149, 1179, 1278, 1719, 1731
FokI	GGATG	1 cut(s)	224
FriOI	GRGCYC	1 cut(s)	1390
Fsp4HI	GCNGC	9 cut(s)	17, 24, 276, 552, 1149, 1179, 1278, 1719, 1731
FspBI	CTAG	5 cut(s)	44, 1253, 1367, 1727, 1794
GlaI	GCGC	1 cut(s)	1365
GluI	GCNGC	9 cut(s)	17, 24, 276, 552, 1149, 1179, 1278, 1719, 1731
GsaI	CCCAGC	2 cut(s)	1514, 1597
GsuI	CTGGAG	5 cut(s)	244, 873, 1169, 1404, 1662
HaeIII	GGCC	4 cut(s)	251, 935, 986, 1316
HapII	CCGG	3 cut(s)	240, 536, 1555
HgaI	GACGC	1 cut(s)	59
HhaI	GCGC	1 cut(s)	1366
Hin1I	GRCGYC	1 cut(s)	51
Hin6I	GCGC	1 cut(s)	1364
HinP1I	GCGC	1 cut(s)	1364
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HpaII	CCGG	3 cut(s)	240, 536, 1555
HphI	GGTGA	5 cut(s)	533, 667, 872, 968, 1672
Hpy166II	GTNNAC	1 cut(s)	49
Hpy188I	TCNGA	5 cut(s)	112, 231, 378, 673, 869
Hpy188III	TCNNGA	8 cut(s)	160, 261, 446, 536, 717, 1066, 1166, 1383
Hpy8I	GTNNAC	1 cut(s)	49
Hpy99I	CGWCG	2 cut(s)	53, 74
HpyAV	CCTTC	9 cut(s)	97, 103, 115, 166, 166, 646, 822, 1796, 1800
HpyCH4III	ACNGT	4 cut(s)	827, 1127, 1204, 1212
HpyCH4IV	ACGT	2 cut(s)	478, 483
HpyF10VI	GCNNNNNNNGC	6 cut(s)	264, 473, 548, 1520, 1529, 1727
HpySE526I	ACGT	2 cut(s)	478, 483
Hsp92I	GRCGYC	1 cut(s)	51
HspAI	GCGC	1 cut(s)	1364
Kpn2I	TCCGGA	1 cut(s)	535
KpnI	GGTACC	1 cut(s)	1106
Kzo9I	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
LmnI	GCTCC	8 cut(s)	40, 223, 263, 361, 1385, 1537, 1580, 1621
Lsp1109I	GCAGC	8 cut(s)	3, 262, 538, 1135, 1165, 1264, 1705, 1717
MabI	ACCWGGT	1 cut(s)	952
MaeI	CTAG	5 cut(s)	44, 1253, 1367, 1727, 1794
MaeII	ACGT	2 cut(s)	478, 483
MaeIII	GTNAC	7 cut(s)	197, 291, 479, 766, 1212, 1547, 1818
MalI	GATC	5 cut(s)	525, 866, 1447, 1676, 1866
MboI	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
MboII	GAAGA	6 cut(s)	606, 670, 1081, 1106, 1861, 1871
MflI	RGATCY	1 cut(s)	1445
MhlI	GDGCHC	2 cut(s)	1260, 1390
MlsI	TGGCCA	1 cut(s)	935
MluNI	TGGCCA	1 cut(s)	935
MlyI	GAGTC	8 cut(s)	74, 243, 395, 662, 864, 1106, 1847, 1923
Mox20I	TGGCCA	1 cut(s)	935
Mph1103I	ATGCAT	5 cut(s)	330, 442, 715, 945, 1960
MroI	TCCGGA	1 cut(s)	535
MroXI	GAANNNNTTC	1 cut(s)	1059
MscI	TGGCCA	1 cut(s)	935
MseI	TTAA	3 cut(s)	948, 1622, 1853
MslI	CAYNNNNRTG	2 cut(s)	1588, 1953
Msp20I	TGGCCA	1 cut(s)	935
MspA1I	CMGCKG	1 cut(s)	833
MspI	CCGG	3 cut(s)	240, 536, 1555
MspR9I	CCNGG	2 cut(s)	954, 1641
Mva1269I	GAATGC	1 cut(s)	1042
MvaI	CCWGG	2 cut(s)	954, 1641
MwoI	GCNNNNNNNGC	6 cut(s)	264, 473, 548, 1520, 1529, 1727
NcoI	CCATGG	1 cut(s)	905
NdeI	CATATG	1 cut(s)	1141
NdeII	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
NlaIV	GGNNCC	3 cut(s)	1104, 1387, 1447
NmuCI	GTSAC	2 cut(s)	197, 479
NsiI	ATGCAT	5 cut(s)	330, 442, 715, 945, 1960
NspI	RCATGY	4 cut(s)	943, 1220, 1277, 1635
PaeI	GCATGC	2 cut(s)	1277, 1635
PceI	AGGCCT	1 cut(s)	986
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1042
PdmI	GAANNNNTTC	1 cut(s)	1059
PfeI	GAWTC	2 cut(s)	282, 403
PflFI	GACNNNGTC	1 cut(s)	485
PkrI	GCNGC	9 cut(s)	18, 25, 277, 553, 1150, 1180, 1279, 1720, 1732
PleI	GAGTC	8 cut(s)	74, 242, 394, 662, 864, 1106, 1846, 1922
PmaCI	CACGTG	1 cut(s)	479
PmlI	CACGTG	1 cut(s)	479
PpsI	GAGTC	8 cut(s)	74, 242, 394, 662, 864, 1106, 1846, 1922
Ppu21I	YACGTR	1 cut(s)	479
Psp6I	CCWGG	2 cut(s)	952, 1639
PspCI	CACGTG	1 cut(s)	479
PspEI	GGTNACC	1 cut(s)	1818
PspFI	CCCAGC	2 cut(s)	1510, 1593
PspGI	CCWGG	2 cut(s)	952, 1639
PspN4I	GGNNCC	3 cut(s)	1104, 1387, 1447
PspPI	GGNCC	2 cut(s)	827, 1314
PsuI	RGATCY	1 cut(s)	1445
PsyI	GACNNNGTC	1 cut(s)	485
PvuII	CAGCTG	1 cut(s)	833
RsaI	GTAC	4 cut(s)	191, 590, 1104, 1395
RsaNI	GTAC	4 cut(s)	190, 589, 1103, 1394
RseI	CAYNNNNRTG	2 cut(s)	1588, 1953
SalI	GTCGAC	1 cut(s)	47
SaqAI	TTAA	3 cut(s)	948, 1622, 1853
SatI	GCNGC	9 cut(s)	17, 24, 276, 552, 1149, 1179, 1278, 1719, 1731
Sau3AI	GATC	5 cut(s)	523, 864, 1445, 1674, 1864
Sau96I	GGNCC	2 cut(s)	827, 1314
SchI	GAGTC	8 cut(s)	74, 243, 395, 662, 864, 1106, 1847, 1923
ScrFI	CCNGG	2 cut(s)	954, 1641
SduI	GDGCHC	2 cut(s)	1260, 1390
SexAI	ACCWGGT	1 cut(s)	952
SfcI	CTRYAG	1 cut(s)	1203
SinI	GGWCC	1 cut(s)	827
SmiMI	CAYNNNNRTG	2 cut(s)	1588, 1953
SphI	GCATGC	2 cut(s)	1277, 1635
SseBI	AGGCCT	1 cut(s)	986
SsiI	CCGC	8 cut(s)	19, 23, 26, 38, 215, 751, 1918, 1978
SspI	AATATT	2 cut(s)	1008, 1198
SspMI	CTAG	5 cut(s)	44, 1253, 1367, 1727, 1794
StuI	AGGCCT	1 cut(s)	986
StyD4I	CCNGG	2 cut(s)	952, 1639
StyI	CCWWGG	2 cut(s)	905, 1467
TaaI	ACNGT	4 cut(s)	827, 1127, 1204, 1212
TaiI	ACGT	2 cut(s)	481, 486
TaqI	TCGA	2 cut(s)	48, 887
TatI	WGTACW	2 cut(s)	588, 1393
TauI	GCSGC	1 cut(s)	26
TfiI	GAWTC	2 cut(s)	282, 403
Tru1I	TTAA	3 cut(s)	948, 1622, 1853
Tru9I	TTAA	3 cut(s)	948, 1622, 1853
TscAI	CASTG	4 cut(s)	106, 292, 433, 826
TseFI	GTSAC	2 cut(s)	197, 479
TseI	GCWGC	8 cut(s)	16, 275, 551, 1148, 1178, 1277, 1718, 1730
Tsp45I	GTSAC	2 cut(s)	197, 479
TspDTI	ATGAA	6 cut(s)	85, 1032, 1049, 1068, 1420, 1870
TspGWI	ACGGA	3 cut(s)	910, 1006, 1347
TspRI	CASTG	4 cut(s)	106, 292, 433, 826
Tth111I	GACNNNGTC	1 cut(s)	485
VpaK11BI	GGWCC	1 cut(s)	827
XagI	CCTNNNNNAGG	1 cut(s)	991
XapI	RAATTY	6 cut(s)	372, 900, 1019, 1055, 1481, 1928
XceI	RCATGY	4 cut(s)	943, 1220, 1277, 1635
XcmI	CCANNNNNNNNNTGG	1 cut(s)	960
XmiI	GTMKAC	1 cut(s)	48
XmnI	GAANNNNTTC	1 cut(s)	1059
XspI	CTAG	5 cut(s)	44, 1253, 1367, 1727, 1794
Zsp2I	ATGCAT	5 cut(s)	330, 442, 715, 945, 1960

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.