Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Reverse (-)

38739579 .. 38744842

Gene Structure 5264 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1277900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1809 bp

ATGGATTTGACGGAGCTGAAAACGGCGGTGGAGGAAGCGGAGCTCGTCGACGCCCACGCCCACAACATCGTCGCCGCCGACTCCACCATCCCCTTCATCAGCTGCTTCTCCGAAGCCACCGGCGACGCCTTATCCTACGCACCCCATTCCCTCTCCTTCAAGAGGAATTTGAAGGATGTCGCCGAGCTCTACGGATCGGAAAAAACCTTGCATGGAGTAGAAGAACACCGGAGACTCGCCGGATTGCAGGCAATTAGCTTGGCGTGCTTCACAGCTGCAAGAATCTCTGTCATTCTCATTGATGATGGGTTGAGGTTTGACAAGAAGCTCGACATAGATTGGCATAAAAATTTCGCACCGGTTGTTGGCCGGATTTTGAGAATTGAGTATTTGGCTGAGGAAATTCTCAATGAAGAATTGCCCAGTGGATCGTCTTGGACACTGGATTTGTTCACCGAAAAGTTTATTTTTGGCTTGAAAAGTATAGCTGCATATCGCAGTGGCCTGGAAATTAATACAAATGTTACTAGGGAAGATGCTGAGGAAGGTCTTGCTGATGTGTTACGTGCTGCACAGCCCGTTCGCATATCAAATAAAAGTTTTATCGATTTTATCTTCACTCGTAGTTTGGAGGTTGCTCTGCTTTTTGACTTGCCCATGCAGATACATACGGGTTTTGGTGATAAAGATTTGGATATGCGGCTATCCAATCCTCTTCATCTTCGGGATGTTCTTGAGGACAAGAGATTTTCTAAATGCCATATAGTTCTTTTACATGCATCCTACCCATTTTCAAAAGAAGCATCATACCTAGCCTCCATTTATCCCCAGGTGTACCTTGACTTTGGGTTGGCTGTCCCTAAGCTCAGTGTCCATGGGATGATATCTTCTGTCAAGGAACTTTTAGAGCTAGCTCCAATTAAGAAGGTGATGTTTAGCACTGACGGCTACGCATTTCCTGAAACTTTCTACTTAGGTGCAAAAAAAGCACGTGAAGTTGTTTTCTCTGTCCTACGGGATGCTTGCACTGACGGTGATCTCTCAGTCCCTGAAGCTATTGAGGCTGCTAAAGACATATTTTCACAAAATGCGATTCAGTTTTACAAGATTAATTATGCTGTAAAATCTTCTGGTTCAAATAATTATGTATCACTGGACTTCACGAAGGTGAACAGTAATGACTCAGAAAATGATGTCTCACTTGTTCGTGTTATGTGGGGTGATACTTCAGGACAACAGAGATGCCGTGTTGTTCCCAAAAAGCGATTCAATGATGTTGTTATAAAAAATGGTATTGGTTTGACTTTTGCTTCTATGGGAATGACTTCATTAGTTGATGGTCCGGCTGATGAGACTAATTTGACTGGAGTGGGTGAGATCAGACTCATGCCTGATTTATCGACTAAACGGAAAATTCCATGGGTAGAACAAGAGGAGATGGTTTTGGCCGATATGCATCTAAAACCGGGTGAAGCATGGGAATACTGCCCAAGAGAAGCCTTACGTAGGGTTTCAAAAATTTTGAAAGATGATTTTAACTTGGATATGAATGCAGGATTTGAGAATGAGTTTTTTCTCTTGAAAGGTATACTAAGGGATGGGAAGGAGGAATTGGTCCCATTTGACTCAACACCCTACTGCTCTGCATCAGCATATGATTCTGCTTCTTATTTATTTCACGAAATTGTTCCTGCTCTTCATTCATTGAATATTACTGTGGAGCAGGTGAAGAAAACTCATGATATTGTTGCTTATTGTTTTTATAATTTTGTAGGACGAACTTTTCCATCAGAATACCGTGCTGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

603

Amino Acids

67.5

Weight (kDa)

5.26

Isoelectric Point (pI)

42.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_2	PF04909	216 - 369	8.4e-15	Amidohydrolase
Gln-synt_N_2	PF16952	395 - 474	6.6e-07	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	495 - 577	2.7e-08	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1283, 1764
AarI	CACCTGC	1 cut(s)	1714
Acc36I	ACCTGC	1 cut(s)	1714
AccI	GTMKAC	2 cut(s)	48, 1588
AciI	CCGC	4 cut(s)	26, 38, 75, 700
AclWI	GGATC	2 cut(s)	202, 436
AcoI	YGGCCR	2 cut(s)	367, 1446
AcsI	RAATTY	5 cut(s)	166, 349, 402, 1413, 1518
AcuI	CTGAAG	2 cut(s)	1071, 1212
AcvI	CACGTG	1 cut(s)	992
AcyI	GRCGYC	2 cut(s)	51, 126
AfaI	GTAC	1 cut(s)	836
AfiI	CCNNNNNNNGG	3 cut(s)	162, 365, 1506
AgeI	ACCGGT	1 cut(s)	358
AjnI	CCWGG	2 cut(s)	504, 828
AjuI	GAANNNNNNNTTGG	4 cut(s)	1250, 1282, 1595, 1627
AleI	CACNNNNGTG	1 cut(s)	1166
Alw21I	GWGCWC	2 cut(s)	45, 189
Alw26I	GTCTC	3 cut(s)	226, 1201, 1346
AlwI	GGATC	2 cut(s)	202, 436
AlwNI	CAGNNNCTG	1 cut(s)	1049
AoxI	GGCC	3 cut(s)	367, 502, 1446
ApeKI	GCWGC	5 cut(s)	102, 275, 488, 569, 1064
ApoI	RAATTY	5 cut(s)	166, 349, 402, 1413, 1518
ArsI	GACNNNNNNTTYG	2 cut(s)	430, 462
AseI	ATTAAT	2 cut(s)	513, 1110
AsiGI	ACCGGT	1 cut(s)	358
Asp700I	GAANNNNTTC	2 cut(s)	1324, 1686
AspS9I	GGNCC	2 cut(s)	1340, 1615
AsuC2I	CCSGG	1 cut(s)	1467
AsuHPI	GGTGA	9 cut(s)	445, 692, 940, 1046, 1180, 1232, 1385, 1481, 1738
AsuNHI	GCTAGC	1 cut(s)	910
AvaII	GGWCC	2 cut(s)	1340, 1615
BanII	GRGCYC	2 cut(s)	45, 189
BbrPI	CACGTG	1 cut(s)	992
Bbv12I	GWGCWC	2 cut(s)	45, 189
BbvCI	CCTCAGC	2 cut(s)	396, 540
BbvI	GCAGC	5 cut(s)	89, 262, 475, 556, 1051
BccI	CCATC	6 cut(s)	95, 299, 1331, 1432, 1592, 1795
BceAI	ACGGC	3 cut(s)	39, 961, 1230
BciT130I	CCWGG	2 cut(s)	506, 830
BcnI	CCSGG	1 cut(s)	1467
BcoDI	GTCTC	3 cut(s)	226, 1201, 1346
BfaI	CTAG	3 cut(s)	528, 812, 911
BfuAI	ACCTGC	1 cut(s)	1714
BisI	GCNGC	7 cut(s)	75, 103, 276, 489, 570, 701, 1065
BlsI	GCNGC	7 cut(s)	76, 104, 277, 490, 571, 702, 1066
Bme1390I	CCNGG	3 cut(s)	506, 830, 1467
Bme18I	GGWCC	2 cut(s)	1340, 1615
BmgT120I	GGNCC	2 cut(s)	1340, 1615
BmiI	GGNNCC	1 cut(s)	1617
BmrFI	CCNGG	3 cut(s)	506, 830, 1467
BmrI	ACTGGG	1 cut(s)	417
BmsI	GCATC	7 cut(s)	526, 788, 812, 1009, 1232, 1465, 1655
BmtI	GCTAGC	1 cut(s)	914
BmuI	ACTGGG	1 cut(s)	417
BpmI	CTGGAG	1 cut(s)	1386
Bpu10I	CCTNAGC	3 cut(s)	396, 540, 861
BpuEI	CTTGAG	1 cut(s)	755
BpuMI	CCSGG	1 cut(s)	1467
Bsa29I	ATCGAT	1 cut(s)	606
BsaAI	YACGTR	3 cut(s)	566, 992, 1505
BsaBI	GATNNNNATC	1 cut(s)	1455
BsaHI	GRCGYC	2 cut(s)	51, 126
BsaJI	CCNNGG	3 cut(s)	828, 874, 1418
BsaWI	WCCGGW	2 cut(s)	228, 358
BsaXI	ACNNNNNCTCC	2 cut(s)	800, 830
Bsc4I	CCNNNNNNNGG	3 cut(s)	162, 365, 1506
Bse118I	RCCGGY	2 cut(s)	119, 358
Bse1I	ACTGG	4 cut(s)	423, 447, 1158, 1369
Bse8I	GATNNNNATC	1 cut(s)	1455
BseBI	CCWGG	2 cut(s)	506, 830
BseCI	ATCGAT	1 cut(s)	606
BseDI	CCNNGG	3 cut(s)	828, 874, 1418
BseGI	GGATG	7 cut(s)	87, 181, 733, 779, 885, 1024, 1603
BseJI	GATNNNNATC	1 cut(s)	1455
BseLI	CCNNNNNNNGG	3 cut(s)	162, 365, 1506
BseMII	CTCAG	5 cut(s)	387, 531, 880, 1056, 1197
BseNI	ACTGG	4 cut(s)	423, 447, 1158, 1369
BseRI	GAGGAG	1 cut(s)	1448
BseXI	GCAGC	5 cut(s)	89, 262, 475, 556, 1051
BsgI	GTGCAG	1 cut(s)	555
BshFI	GGCC	3 cut(s)	369, 504, 1448
BshTI	ACCGGT	1 cut(s)	358
BshVI	ATCGAT	1 cut(s)	606
BsiHKAI	GWGCWC	2 cut(s)	45, 189
BsiSI	CCGG	7 cut(s)	120, 229, 240, 359, 370, 1343, 1466
BslFI	GGGAC	3 cut(s)	842, 1031, 1601
BslI	CCNNNNNNNGG	3 cut(s)	162, 365, 1506
BsmAI	GTCTC	3 cut(s)	226, 1201, 1346
BsmFI	GGGAC	3 cut(s)	842, 1031, 1601
BsmI	GAATGC	1 cut(s)	1555
BsnI	GGCC	3 cut(s)	369, 504, 1448
Bsp1286I	GDGCHC	2 cut(s)	45, 189
Bsp143I	GATC	4 cut(s)	194, 428, 1036, 1377
Bsp19I	CCATGG	2 cut(s)	874, 1418
BspACI	CCGC	4 cut(s)	26, 38, 75, 700
BspANI	GGCC	3 cut(s)	369, 504, 1448
BspCNI	CTCAG	5 cut(s)	388, 532, 879, 1055, 1196
BspDI	ATCGAT	1 cut(s)	606
BspHI	TCATGA	1 cut(s)	1738
BspLI	GGNNCC	1 cut(s)	1617
BspMI	ACCTGC	1 cut(s)	1714
BspOI	GCTAGC	1 cut(s)	914
BspPI	GGATC	2 cut(s)	202, 436
BspQI	GCTCTTC	1 cut(s)	1701
BsrFI	RCCGGY	2 cut(s)	119, 358
BsrI	ACTGG	4 cut(s)	423, 447, 1158, 1369
BssAI	RCCGGY	2 cut(s)	119, 358
BssECI	CCNNGG	3 cut(s)	828, 874, 1418
BssMI	GATC	4 cut(s)	194, 428, 1036, 1377
BssNAI	GTATAC	1 cut(s)	1589
BssNI	GRCGYC	2 cut(s)	51, 126
BssT1I	CCWWGG	2 cut(s)	874, 1418
Bst1107I	GTATAC	1 cut(s)	1589
Bst2UI	CCWGG	2 cut(s)	506, 830
Bst4CI	ACNGT	4 cut(s)	1034, 1175, 1717, 1799
Bst6I	CTCTTC	2 cut(s)	720, 1701
BstACI	GRCGYC	2 cut(s)	51, 126
BstBAI	YACGTR	3 cut(s)	566, 992, 1505
BstC8I	GCNNGC	4 cut(s)	249, 265, 912, 1024
BstDEI	CTNAG	8 cut(s)	396, 540, 861, 866, 973, 1042, 1183, 1592
BstDSI	CCRYGG	2 cut(s)	874, 1418
BstENI	CCTNNNNNAGG	2 cut(s)	160, 1504
BstF5I	GGATG	7 cut(s)	87, 181, 733, 779, 885, 1024, 1603
BstKTI	GATC	4 cut(s)	197, 431, 1039, 1380
BstMAI	GTCTC	3 cut(s)	226, 1201, 1346
BstMBI	GATC	4 cut(s)	194, 428, 1036, 1377
BstMWI	GCNNNNNNNGC	4 cut(s)	264, 945, 986, 1061
BstNI	CCWGG	2 cut(s)	506, 830
BstNSI	RCATGY	1 cut(s)	779
BstSCI	CCNGG	3 cut(s)	504, 828, 1465
BstSNI	TACGTA	1 cut(s)	1505
BstV1I	GCAGC	5 cut(s)	89, 262, 475, 556, 1051
BstZ17I	GTATAC	1 cut(s)	1589
Bsu15I	ATCGAT	1 cut(s)	606
BsuRI	GGCC	3 cut(s)	369, 504, 1448
BsuTUI	ATCGAT	1 cut(s)	606
BtgI	CCRYGG	2 cut(s)	874, 1418
BtsCI	GGATG	7 cut(s)	87, 181, 733, 779, 885, 1024, 1603
BtsI	GCAGTG	1 cut(s)	505
BtsIMutI	CAGTG	7 cut(s)	430, 440, 505, 874, 939, 1026, 1151
BveI	ACCTGC	1 cut(s)	1714
Cac8I	GCNNGC	4 cut(s)	249, 265, 912, 1024
CaiI	CAGNNNCTG	1 cut(s)	1049
CciI	TCATGA	1 cut(s)	1738
Cfr10I	RCCGGY	2 cut(s)	119, 358
Cfr13I	GGNCC	2 cut(s)	1340, 1615
ClaI	ATCGAT	1 cut(s)	606
CseI	GACGC	2 cut(s)	59, 134
Csp6I	GTAC	1 cut(s)	835
CspAI	ACCGGT	1 cut(s)	358
CviAII	CATG	8 cut(s)	212, 658, 776, 875, 1387, 1419, 1476, 1739
CviQI	GTAC	1 cut(s)	835
DdeI	CTNAG	8 cut(s)	396, 540, 861, 866, 973, 1042, 1183, 1592
DpnI	GATC	4 cut(s)	196, 430, 1038, 1379
DpnII	GATC	4 cut(s)	194, 428, 1036, 1377
EaeI	YGGCCR	2 cut(s)	367, 1446
Eam1104I	CTCTTC	2 cut(s)	720, 1701
EarI	CTCTTC	2 cut(s)	720, 1701
Ecl136II	GAGCTC	2 cut(s)	43, 187
Eco105I	TACGTA	1 cut(s)	1505
Eco130I	CCWWGG	2 cut(s)	874, 1418
Eco24I	GRGCYC	2 cut(s)	45, 189
Eco32I	GATATC	1 cut(s)	885
Eco47I	GGWCC	2 cut(s)	1340, 1615
Eco53kI	GAGCTC	2 cut(s)	43, 187
Eco57I	CTGAAG	2 cut(s)	1071, 1212
Eco72I	CACGTG	1 cut(s)	992
EcoICRI	GAGCTC	2 cut(s)	43, 187
EcoNI	CCTNNNNNAGG	2 cut(s)	160, 1504
EcoRII	CCWGG	2 cut(s)	504, 828
EcoRV	GATATC	1 cut(s)	885
EcoT14I	CCWWGG	2 cut(s)	874, 1418
EcoT22I	ATGCAT	2 cut(s)	781, 1458
EcoT38I	GRGCYC	2 cut(s)	45, 189
ErhI	CCWWGG	2 cut(s)	874, 1418
FaeI	CATG	8 cut(s)	215, 661, 779, 878, 1390, 1422, 1479, 1742
FaqI	GGGAC	3 cut(s)	842, 1031, 1601
FatI	CATG	8 cut(s)	211, 657, 775, 874, 1386, 1418, 1475, 1738
FauNDI	CATATG	1 cut(s)	1654
FblI	GTMKAC	2 cut(s)	48, 1588
Fnu4HI	GCNGC	7 cut(s)	75, 103, 276, 489, 570, 701, 1065
FokI	GGATG	7 cut(s)	74, 188, 740, 766, 892, 1031, 1610
FriOI	GRGCYC	2 cut(s)	45, 189
Fsp4HI	GCNGC	7 cut(s)	75, 103, 276, 489, 570, 701, 1065
FspBI	CTAG	3 cut(s)	528, 812, 911
GluI	GCNGC	7 cut(s)	75, 103, 276, 489, 570, 701, 1065
GsuI	CTGGAG	1 cut(s)	1386
HaeIII	GGCC	3 cut(s)	369, 504, 1448
HapII	CCGG	7 cut(s)	120, 229, 240, 359, 370, 1343, 1466
HgaI	GACGC	2 cut(s)	59, 134
Hin1I	GRCGYC	2 cut(s)	51, 126
Hin1II	CATG	8 cut(s)	215, 661, 779, 878, 1390, 1422, 1479, 1742
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HinfI	GANTC	9 cut(s)	80, 234, 282, 1093, 1181, 1266, 1383, 1625, 1658
HpaII	CCGG	7 cut(s)	120, 229, 240, 359, 370, 1343, 1466
HphI	GGTGA	9 cut(s)	445, 692, 940, 1046, 1180, 1232, 1385, 1481, 1738
Hpy166II	GTNNAC	5 cut(s)	49, 453, 835, 1171, 1589
Hpy188I	TCNGA	5 cut(s)	112, 199, 1186, 1382, 1792
Hpy188III	TCNNGA	9 cut(s)	160, 725, 734, 959, 1162, 1230, 1579, 1679, 1739
Hpy8I	GTNNAC	5 cut(s)	49, 453, 835, 1171, 1589
Hpy99I	CGWCG	4 cut(s)	50, 53, 74, 128
HpyAV	CCTTC	7 cut(s)	103, 166, 166, 539, 919, 1159, 1597
HpyCH4III	ACNGT	4 cut(s)	1034, 1175, 1717, 1799
HpyCH4IV	ACGT	3 cut(s)	565, 991, 1504
HpyF10VI	GCNNNNNNNGC	4 cut(s)	264, 945, 986, 1061
HpyF3I	CTNAG	8 cut(s)	396, 540, 861, 866, 973, 1042, 1183, 1592
HpySE526I	ACGT	3 cut(s)	565, 991, 1504
Hsp92I	GRCGYC	2 cut(s)	51, 126
Hsp92II	CATG	8 cut(s)	215, 661, 779, 878, 1390, 1422, 1479, 1742
Kzo9I	GATC	4 cut(s)	194, 428, 1036, 1377
LguI	GCTCTTC	1 cut(s)	1701
LmnI	GCTCC	4 cut(s)	13, 40, 919, 1720
Lsp1109I	GCAGC	5 cut(s)	89, 262, 475, 556, 1051
LweI	GCATC	7 cut(s)	526, 788, 812, 1009, 1232, 1465, 1655
MaeI	CTAG	3 cut(s)	528, 812, 911
MaeII	ACGT	3 cut(s)	565, 991, 1504
MaeIII	GTNAC	2 cut(s)	523, 561
MalI	GATC	4 cut(s)	196, 430, 1038, 1379
MboI	GATC	4 cut(s)	194, 428, 1036, 1377
MhlI	GDGCHC	2 cut(s)	45, 189
MlyI	GAGTC	5 cut(s)	74, 228, 1175, 1377, 1619
Mph1103I	ATGCAT	2 cut(s)	781, 1458
MroXI	GAANNNNTTC	2 cut(s)	1324, 1686
MseI	TTAA	4 cut(s)	513, 921, 1110, 1536
MslI	CAYNNNNRTG	1 cut(s)	1166
MspA1I	CMGCKG	2 cut(s)	102, 275
MspI	CCGG	7 cut(s)	120, 229, 240, 359, 370, 1343, 1466
MspR9I	CCNGG	3 cut(s)	506, 830, 1467
Mva1269I	GAATGC	1 cut(s)	1555
MvaI	CCWGG	2 cut(s)	506, 830
MwoI	GCNNNNNNNGC	4 cut(s)	264, 945, 986, 1061
NciI	CCSGG	1 cut(s)	1467
NcoI	CCATGG	2 cut(s)	874, 1418
NdeI	CATATG	1 cut(s)	1654
NdeII	GATC	4 cut(s)	194, 428, 1036, 1377
NheI	GCTAGC	1 cut(s)	910
NlaIII	CATG	8 cut(s)	215, 661, 779, 878, 1390, 1422, 1479, 1742
NlaIV	GGNNCC	1 cut(s)	1617
NmeAIII	GCCGAG	1 cut(s)	208
NsiI	ATGCAT	2 cut(s)	781, 1458
NspI	RCATGY	1 cut(s)	779
OliI	CACNNNNGTG	1 cut(s)	1166
PagI	TCATGA	1 cut(s)	1738
PaqCI	CACCTGC	1 cut(s)	1714
PciSI	GCTCTTC	1 cut(s)	1701
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1555
PdmI	GAANNNNTTC	2 cut(s)	1324, 1686
PfeI	GAWTC	4 cut(s)	282, 1093, 1266, 1658
PinAI	ACCGGT	1 cut(s)	358
PkrI	GCNGC	7 cut(s)	76, 104, 277, 490, 571, 702, 1066
PleI	GAGTC	5 cut(s)	74, 228, 1175, 1377, 1619
PmaCI	CACGTG	1 cut(s)	992
PmlI	CACGTG	1 cut(s)	992
PpsI	GAGTC	5 cut(s)	74, 228, 1175, 1377, 1619
Ppu21I	YACGTR	3 cut(s)	566, 992, 1505
PshBI	ATTAAT	2 cut(s)	513, 1110
PsiI	TTATAA	2 cut(s)	1283, 1764
Psp124BI	GAGCTC	2 cut(s)	45, 189
Psp6I	CCWGG	2 cut(s)	504, 828
PspCI	CACGTG	1 cut(s)	992
PspGI	CCWGG	2 cut(s)	504, 828
PspN4I	GGNNCC	1 cut(s)	1617
PspPI	GGNCC	2 cut(s)	1340, 1615
PstNI	CAGNNNCTG	1 cut(s)	1049
PvuII	CAGCTG	2 cut(s)	102, 275
RsaI	GTAC	1 cut(s)	836
RsaNI	GTAC	1 cut(s)	835
RseI	CAYNNNNRTG	1 cut(s)	1166
SacI	GAGCTC	2 cut(s)	45, 189
SalI	GTCGAC	1 cut(s)	47
SapI	GCTCTTC	1 cut(s)	1701
SaqAI	TTAA	4 cut(s)	513, 921, 1110, 1536
SatI	GCNGC	7 cut(s)	75, 103, 276, 489, 570, 701, 1065
Sau3AI	GATC	4 cut(s)	194, 428, 1036, 1377
Sau96I	GGNCC	2 cut(s)	1340, 1615
SchI	GAGTC	5 cut(s)	74, 228, 1175, 1377, 1619
ScrFI	CCNGG	3 cut(s)	506, 830, 1467
SduI	GDGCHC	2 cut(s)	45, 189
SfaNI	GCATC	7 cut(s)	526, 788, 812, 1009, 1232, 1465, 1655
SgrAI	CRCCGGYG	1 cut(s)	119
SgrDI	CGTCGACG	1 cut(s)	47
SinI	GGWCC	2 cut(s)	1340, 1615
SmiMI	CAYNNNNRTG	1 cut(s)	1166
SmlI	CTYRAG	1 cut(s)	734
SmoI	CTYRAG	1 cut(s)	734
SnaBI	TACGTA	1 cut(s)	1505
SsiI	CCGC	4 cut(s)	26, 38, 75, 700
SspI	AATATT	1 cut(s)	1711
SspMI	CTAG	3 cut(s)	528, 812, 911
SstI	GAGCTC	2 cut(s)	45, 189
StyD4I	CCNGG	3 cut(s)	504, 828, 1465
StyI	CCWWGG	2 cut(s)	874, 1418
TaaI	ACNGT	4 cut(s)	1034, 1175, 1717, 1799
TaiI	ACGT	3 cut(s)	568, 994, 1507
TaqI	TCGA	4 cut(s)	48, 330, 606, 1400
TauI	GCSGC	2 cut(s)	77, 703
TfiI	GAWTC	4 cut(s)	282, 1093, 1266, 1658
Tru1I	TTAA	4 cut(s)	513, 921, 1110, 1536
Tru9I	TTAA	4 cut(s)	513, 921, 1110, 1536
TscAI	CASTG	7 cut(s)	430, 447, 505, 874, 946, 1033, 1158
TseI	GCWGC	5 cut(s)	102, 275, 488, 569, 1064
TspDTI	ATGAA	7 cut(s)	85, 426, 707, 1317, 1562, 1688, 1692
TspGWI	ACGGA	3 cut(s)	26, 207, 1423
TspRI	CASTG	7 cut(s)	430, 447, 505, 874, 946, 1033, 1158
VpaK11BI	GGWCC	2 cut(s)	1340, 1615
VspI	ATTAAT	2 cut(s)	513, 1110
XagI	CCTNNNNNAGG	2 cut(s)	160, 1504
XapI	RAATTY	5 cut(s)	166, 349, 402, 1413, 1518
XceI	RCATGY	1 cut(s)	779
XmiI	GTMKAC	2 cut(s)	48, 1588
XmnI	GAANNNNTTC	2 cut(s)	1324, 1686
XspI	CTAG	3 cut(s)	528, 812, 911
Zsp2I	ATGCAT	2 cut(s)	781, 1458

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.