Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Forward (+)

32814102 .. 32821674

Gene Structure 7573 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00410370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2502 bp

ATGGATTTCACGACGCTCCGGGCGGCGGTGGAGGAGGCGGAGCTAGTCGACGCCCACGCCCACAACATCGTCGCCGTCGACTCCTCCTTCCCCTTCATCAGCGCCTTCTCCGAAGCCAACGCCGACGCCTTATCCTACGCACCCCATTCCCTCTCCTTCAAGAGAAGTTTGAAGGAGGTTGCGGAGCTTTACGGTTGTGAGAAGGCCTTGGATGCGGTTGAGGAGCATCGGAGAGTCGCCGGATTAGAGGCGATTAGCTCCCGATGCTTTGAGGCTGCGAGAATCTCCGCATTGCTTATTGATGATGGACTGAGGCTGGACAAAATGCATGACGTAGATTGGCACAAGAGTTTCGCGCCTGTGGTTGGGAGGATTCTCAGGATTGAGTTCCTGGCTGAGCAGATTCTTAATGAGGAGTTGCCGGGTGGATCTTCTTGGACATTGGATGCGTTCACTGAAGCATTTATTTTTGGCTTGAAAAGTATAGCTGCATATCGCAGCGGTCTGGAAATTTGTACACATGTCACTAGGAAAGATGTTGAGGAAGGTCTTTCTGAGGTCATACAGGCTGGAAAGCCTGTTCGCATATCAAATAAAAGTTTTATCGACTACATTTTCACTCGTAGTTTGGAGGTTGCTCTACTTTTTGACTTGCCAATGCAGATACATACAGGTTTTGGCGATAAAGATTTGGACTTGAGGCTATCCAATCCCCTTCATCTCCGGGCTGTGCTTGAGGACAAGAGATTTTCTAAAAGCCGCATTGTTCTTTTACATGCATCCTACCCGTTTTCAAAGGAAGCATCATATCTATCCTCTGTTTATCCCCAGGTGGTCTACCTCGACTTTGGCTTGGCTATACCCAAGCTCAGTGTCCACGGGATGGTATCTTCACTAAAAGAACTTTTGGAGCTTGCTCCAATTAAGAAGGTCATGTTCAGCACTGATGGATATGCATTTCCTGAAACTTTCTACTTAGGTGCAAAAAAGGCACGTGAAGTTGTCTCTTCAGTTCTATGTGATGCCTGTGCCGATGGTGATCTCTCAATTCCGGAAGCTATTGAGGCTGCTAAAGACATCTTTTCACAAAATGCAGTTCAGTTTTACAAGATTGATCGTGCTGTCAAATCTTCTGGTTCAGCCAATAGTGTATCCTCTAACTTTGTGAAGGTGAAGAGTAATGACTCGGAAAATCATGTCTTACTTGTTCGTGTTTTCTGGGCTGATGCATCAGGACAACAAAGATGTCGTGTTGTTCCTGCTAAGCGGTTCAATGACATCGTTACAAAAAATGGCATTGGTTTGACTTTTGCTTGTATGGGAATGACTTCTTTCACTGATGGTCCAGCTGATGAGACTAATTTGACTGGAGTGGGTGAGATCAGACTCATGCCTGATTTATCAACTAAACGGAGAATTCCATGGGTAGAACAAGAGGAAATGGTTTTGGCCAACATGCATCTTAAACCAGGTGAAGCATGGGAATACTGCCCAAGAGAGGCCTTACGGAGGGTTTCAAAAATATTGAAAGATGAATTCAATTTGGTAATGAATGCAGGATTTGAAAATGAATTTTTTCTCTTGAAGAGTATACTAAGGGATAGGAAAGAAGAATGGGTACCATTTGACTCAACACTCTACTGTTCCCCATCATCATATGATGCTGCTTCCTCTTTGTTTCATGAAGTTCTTGCTGCTCTCCAGTCCTTGAATATTACTGTAGAACAGTTACATGCAGAAAGTGGGAAAGGTCAATTTGAGGTGGTTCTAGGGCACACAGCTTGCCAGGATGCTGCTGACAACTTGATTTATACCCGTGAAGTTATTAGGGCCATTGCAAGGAAACACGGATTGTTGGCAACCTTTGTCCCAAAGTATGCACTAGATGAGATTGGCTCTGGAGCCCATGTACATCTCAGTTTGTGGCAGAATGGCAAAAATGTATTCATGGCATCTGGTGGATCCTCTCAGCATGGAATGTCCAAGGTTGGGGAGGAATTTATGGCAGGGGTTTTATATCATCTCCCAGCAATTTTGGCATTTACAGCTCCAATTCCAAATAGTTATGACCGGATACAACCTAATACTTGGAGTGGAGCATACAAGTGCTGGGGAAAAGAAAACAGGGAAGCTGCATTAAGAACTGCATGCCCACCTGGAATTCAAGCTGGTCTGGTGAGCAACTTTGAGATCAAGTCATTTGATGGGTGTGCAAATCCACACTTGGGCTTGGCTGCAATACTAGCTGCTGGCATTGATGGTCTTAGGAGACATCTTTGTCTCCCTGAACCTATTGATACAAATCCTTCTAGCCTTGAAGGAGAACTACAAAGGTTACCAACTTCACTTTTGGAGTCATTAGAAGCTTTAAAGGAAGATGGTCTCTTCAAAGAGTTCATTGGAGAAAAGCTATTGGTTGCCATAAAGGGAGTCCGCAAGGCAGAATTTGACTACTACGAGAAGCACAAAGATGCATACAAGCAACTTATACACCGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

833

Amino Acids

92.35

Weight (kDa)

5.78

Isoelectric Point (pI)

43.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_2	PF04909	216 - 370	7.6e-11	Amidohydrolase
Gln-synt_N_2	PF16952	390 - 475	8.2e-07	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	496 - 825	1.1e-90	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2277
Acc65I	GGTACC	1 cut(s)	1618
AccB1I	GGYRCC	1 cut(s)	1618
AccB7I	CCANNNNNTGG	1 cut(s)	883
AccI	GTMKAC	4 cut(s)	48, 78, 837, 1591
AccII	CGCG	1 cut(s)	356
AccIII	TCCGGA	1 cut(s)	1051
AclWI	GGATC	3 cut(s)	436, 1956, 1969
AcoI	YGGCCR	1 cut(s)	1449
AcsI	RAATTY	7 cut(s)	510, 1416, 1535, 1571, 1997, 2160, 2444
AcuI	CTGAAG	2 cut(s)	477, 993
AcvI	CACGTG	1 cut(s)	995
AcyI	GRCGYC	2 cut(s)	51, 126
AfaI	GTAC	3 cut(s)	517, 1620, 1911
AfiI	CCNNNNNNNGG	8 cut(s)	25, 365, 883, 1498, 1509, 1839, 1989, 2225
AflIII	ACRYGT	1 cut(s)	520
AjnI	CCWGG	5 cut(s)	390, 828, 1468, 1785, 2155
AloI	GAACNNNNNNTCC	2 cut(s)	564, 596
Alw26I	GTCTC	5 cut(s)	1009, 1349, 2263, 2285, 2387
AlwI	GGATC	3 cut(s)	436, 1956, 1969
Aor13HI	TCCGGA	1 cut(s)	1051
AoxI	GGCC	4 cut(s)	204, 1449, 1500, 1830
ApoI	RAATTY	7 cut(s)	510, 1416, 1535, 1571, 1997, 2160, 2444
Asp700I	GAANNNNTTC	2 cut(s)	1327, 1575
Asp718I	GGTACC	1 cut(s)	1618
AspLEI	GCGC	2 cut(s)	104, 358
AspS9I	GGNCC	2 cut(s)	1343, 1830
AsuC2I	CCSGG	3 cut(s)	20, 423, 725
AsuHPI	GGTGA	5 cut(s)	1049, 1183, 1388, 1484, 2188
AvaII	GGWCC	1 cut(s)	1343
BaeGI	GKGCMC	1 cut(s)	1776
BalI	TGGCCA	1 cut(s)	1451
BamHI	GGATCC	1 cut(s)	1961
BanI	GGYRCC	1 cut(s)	1618
BanII	GRGCYC	1 cut(s)	1906
BarI	GAAGNNNNNNTAC	4 cut(s)	1602, 1634, 2310, 2342
BbrPI	CACGTG	1 cut(s)	995
BccI	CCATC	9 cut(s)	299, 877, 941, 1028, 1334, 1657, 2198, 2252, 2372
BceAI	ACGGC	1 cut(s)	59
BciT130I	CCWGG	5 cut(s)	392, 830, 1470, 1787, 2157
BciVI	GTATCC	2 cut(s)	1162, 2067
BcnI	CCSGG	3 cut(s)	20, 423, 725
BcoDI	GTCTC	5 cut(s)	1009, 1349, 2263, 2285, 2387
BfaI	CTAG	6 cut(s)	44, 528, 1769, 1883, 2243, 2310
BfmI	CTRYAG	1 cut(s)	1719
BfoI	RGCGCY	1 cut(s)	105
BfuI	GTATCC	2 cut(s)	1162, 2067
BlpI	GCTNAGC	2 cut(s)	396, 1263
Bme1390I	CCNGG	8 cut(s)	20, 392, 423, 725, 830, 1470, 1787, 2157
Bme18I	GGWCC	1 cut(s)	1343
BmgT120I	GGNCC	2 cut(s)	1343, 1830
BmiI	GGNNCC	3 cut(s)	1620, 1903, 1963
BmrFI	CCNGG	8 cut(s)	20, 392, 423, 725, 830, 1470, 1787, 2157
BplI	GAGNNNNNCTC	2 cut(s)	1880, 1912
BpmI	CTGGAG	3 cut(s)	1389, 1685, 1920
Bpu1102I	GCTNAGC	2 cut(s)	396, 1263
BpuEI	CTTGAG	2 cut(s)	718, 755
BpuMI	CCSGG	3 cut(s)	20, 423, 725
BsaAI	YACGTR	1 cut(s)	995
BsaBI	GATNNNNATC	2 cut(s)	1038, 2301
BsaHI	GRCGYC	2 cut(s)	51, 126
BsaI	GGTCTC	1 cut(s)	2387
BsaJI	CCNNGG	5 cut(s)	207, 828, 877, 1421, 1983
BsaWI	WCCGGW	2 cut(s)	1051, 2070
BsaXI	ACNNNNNCTCC	2 cut(s)	1893, 1923
Bsc4I	CCNNNNNNNGG	8 cut(s)	25, 365, 883, 1498, 1509, 1839, 1989, 2225
Bse1I	ACTGG	2 cut(s)	1372, 1702
Bse3DI	GCAATG	2 cut(s)	290, 1833
Bse8I	GATNNNNATC	2 cut(s)	1038, 2301
BseAI	TCCGGA	1 cut(s)	1051
BseBI	CCWGG	5 cut(s)	392, 830, 1470, 1787, 2157
BseDI	CCNNGG	5 cut(s)	207, 828, 877, 1421, 1983
BseGI	GGATG	5 cut(s)	217, 451, 779, 888, 1795
BseJI	GATNNNNATC	2 cut(s)	1038, 2301
BseLI	CCNNNNNNNGG	8 cut(s)	25, 365, 883, 1498, 1509, 1839, 1989, 2225
BseMI	GCAATG	2 cut(s)	290, 1833
BseMII	CTCAG	7 cut(s)	302, 387, 391, 546, 883, 1930, 1982
BseNI	ACTGG	2 cut(s)	1372, 1702
BseRI	GAGGAG	4 cut(s)	47, 73, 236, 428
BseSI	GKGCMC	1 cut(s)	1776
BseYI	CCCAGC	2 cut(s)	2026, 2109
Bsh1236I	CGCG	1 cut(s)	356
BshFI	GGCC	4 cut(s)	206, 1451, 1502, 1832
BshNI	GGYRCC	1 cut(s)	1618
BsiSI	CCGG	6 cut(s)	19, 240, 422, 724, 1052, 2071
BslFI	GGGAC	1 cut(s)	1853
BslI	CCNNNNNNNGG	8 cut(s)	25, 365, 883, 1498, 1509, 1839, 1989, 2225
BsmAI	GTCTC	5 cut(s)	1009, 1349, 2263, 2285, 2387
BsmFI	GGGAC	1 cut(s)	1853
BsmI	GAATGC	1 cut(s)	1558
BsnI	GGCC	4 cut(s)	206, 1451, 1502, 1832
Bso31I	GGTCTC	1 cut(s)	2387
Bsp1286I	GDGCHC	2 cut(s)	1776, 1906
Bsp13I	TCCGGA	1 cut(s)	1051
Bsp1407I	TGTACA	2 cut(s)	515, 1909
Bsp143I	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
Bsp1720I	GCTNAGC	2 cut(s)	396, 1263
Bsp19I	CCATGG	1 cut(s)	1421
BspANI	GGCC	4 cut(s)	206, 1451, 1502, 1832
BspCNI	CTCAG	7 cut(s)	303, 388, 390, 547, 882, 1929, 1981
BspEI	TCCGGA	1 cut(s)	1051
BspFNI	CGCG	1 cut(s)	356
BspHI	TCATGA	1 cut(s)	1681
BspLI	GGNNCC	3 cut(s)	1620, 1903, 1963
BspPI	GGATC	3 cut(s)	436, 1956, 1969
BspT107I	GGYRCC	1 cut(s)	1618
BspTNI	GGTCTC	1 cut(s)	2387
BsrDI	GCAATG	2 cut(s)	290, 1833
BsrGI	TGTACA	2 cut(s)	515, 1909
BsrI	ACTGG	2 cut(s)	1372, 1702
BssECI	CCNNGG	5 cut(s)	207, 828, 877, 1421, 1983
BssMI	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
BssNAI	GTATAC	1 cut(s)	1592
BssNI	GRCGYC	2 cut(s)	51, 126
BssT1I	CCWWGG	3 cut(s)	207, 1421, 1983
Bst1107I	GTATAC	1 cut(s)	1592
Bst2UI	CCWGG	5 cut(s)	392, 830, 1470, 1787, 2157
Bst4CI	ACNGT	4 cut(s)	194, 1643, 1720, 1728
Bst6I	CTCTTC	4 cut(s)	1012, 1169, 1580, 2390
BstACI	GRCGYC	2 cut(s)	51, 126
BstAUI	TGTACA	2 cut(s)	515, 1909
BstBAI	YACGTR	1 cut(s)	995
BstC8I	GCNNGC	4 cut(s)	915, 1783, 2149, 2251
BstDSI	CCRYGG	2 cut(s)	877, 1421
BstEII	GGTNACC	1 cut(s)	2334
BstENI	CCTNNNNNAGG	1 cut(s)	1507
BstF5I	GGATG	5 cut(s)	217, 451, 779, 888, 1795
BstFNI	CGCG	1 cut(s)	356
BstH2I	RGCGCY	1 cut(s)	105
BstHHI	GCGC	2 cut(s)	104, 358
BstKTI	GATC	6 cut(s)	431, 1042, 1117, 1383, 1964, 2193
BstMAI	GTCTC	5 cut(s)	1009, 1349, 2263, 2285, 2387
BstMBI	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
BstMWI	GCNNNNNNNGC	7 cut(s)	212, 264, 989, 1064, 2036, 2045, 2243
BstNI	CCWGG	5 cut(s)	392, 830, 1470, 1787, 2157
BstNSI	RCATGY	5 cut(s)	524, 779, 1459, 1736, 2151
BstPI	GGTNACC	1 cut(s)	2334
BstSCI	CCNGG	8 cut(s)	18, 390, 421, 723, 828, 1468, 1785, 2155
BstSFI	CTRYAG	1 cut(s)	1719
BstSLI	GKGCMC	1 cut(s)	1776
BstUI	CGCG	1 cut(s)	356
BstX2I	RGATCY	2 cut(s)	428, 1961
BstYI	RGATCY	2 cut(s)	428, 1961
BstZ17I	GTATAC	1 cut(s)	1592
BsuI	GTATCC	2 cut(s)	1162, 2067
BsuRI	GGCC	4 cut(s)	206, 1451, 1502, 1832
BtgI	CCRYGG	2 cut(s)	877, 1421
BtsCI	GGATG	5 cut(s)	217, 451, 779, 888, 1795
BtsIMutI	CAGTG	4 cut(s)	453, 877, 942, 1335
Cac8I	GCNNGC	4 cut(s)	915, 1783, 2149, 2251
CciI	TCATGA	1 cut(s)	1681
CfoI	GCGC	2 cut(s)	104, 358
Cfr13I	GGNCC	2 cut(s)	1343, 1830
CseI	GACGC	3 cut(s)	22, 59, 134
CsiI	ACCWGGT	1 cut(s)	1468
Csp6I	GTAC	3 cut(s)	516, 1619, 1910
CviQI	GTAC	3 cut(s)	516, 1619, 1910
DpnI	GATC	6 cut(s)	430, 1041, 1116, 1382, 1963, 2192
DpnII	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
DraI	TTTAAA	1 cut(s)	2370
DrdI	GACNNNNNNGTC	1 cut(s)	2277
DseDI	GACNNNNNNGTC	1 cut(s)	2277
EaeI	YGGCCR	1 cut(s)	1449
Eam1104I	CTCTTC	4 cut(s)	1012, 1169, 1580, 2390
EarI	CTCTTC	4 cut(s)	1012, 1169, 1580, 2390
EciI	GGCGGA	1 cut(s)	53
Eco130I	CCWWGG	3 cut(s)	207, 1421, 1983
Eco147I	AGGCCT	2 cut(s)	206, 1502
Eco24I	GRGCYC	1 cut(s)	1906
Eco31I	GGTCTC	1 cut(s)	2387
Eco47I	GGWCC	1 cut(s)	1343
Eco57I	CTGAAG	2 cut(s)	477, 993
Eco72I	CACGTG	1 cut(s)	995
Eco91I	GGTNACC	1 cut(s)	2334
EcoNI	CCTNNNNNAGG	1 cut(s)	1507
EcoO65I	GGTNACC	1 cut(s)	2334
EcoRI	GAATTC	3 cut(s)	1416, 1535, 2160
EcoRII	CCWGG	5 cut(s)	390, 828, 1468, 1785, 2155
EcoT14I	CCWWGG	3 cut(s)	207, 1421, 1983
EcoT22I	ATGCAT	6 cut(s)	330, 781, 958, 1231, 1461, 2476
EcoT38I	GRGCYC	1 cut(s)	1906
ErhI	CCWWGG	3 cut(s)	207, 1421, 1983
FaqI	GGGAC	1 cut(s)	1853
FauNDI	CATATG	1 cut(s)	1657
FblI	GTMKAC	4 cut(s)	48, 78, 837, 1591
FokI	GGATG	5 cut(s)	224, 458, 766, 895, 1802
FriOI	GRGCYC	1 cut(s)	1906
FspBI	CTAG	6 cut(s)	44, 528, 1769, 1883, 2243, 2310
GlaI	GCGC	2 cut(s)	103, 357
GsaI	CCCAGC	2 cut(s)	2030, 2113
GsuI	CTGGAG	3 cut(s)	1389, 1685, 1920
HaeII	RGCGCY	1 cut(s)	105
HaeIII	GGCC	4 cut(s)	206, 1451, 1502, 1832
HapII	CCGG	6 cut(s)	19, 240, 422, 724, 1052, 2071
HgaI	GACGC	3 cut(s)	22, 59, 134
HhaI	GCGC	2 cut(s)	104, 358
Hin1I	GRCGYC	2 cut(s)	51, 126
Hin6I	GCGC	2 cut(s)	102, 356
HinP1I	GCGC	2 cut(s)	102, 356
HincII	GTYRAC	2 cut(s)	49, 79
HindII	GTYRAC	2 cut(s)	49, 79
HindIII	AAGCTT	1 cut(s)	2364
HpaII	CCGG	6 cut(s)	19, 240, 422, 724, 1052, 2071
HphI	GGTGA	5 cut(s)	1049, 1183, 1388, 1484, 2188
Hpy166II	GTNNAC	7 cut(s)	49, 79, 453, 518, 838, 877, 1592
Hpy188I	TCNGA	5 cut(s)	112, 231, 556, 1189, 1385
Hpy8I	GTNNAC	7 cut(s)	49, 79, 453, 518, 838, 877, 1592
Hpy99I	CGWCG	5 cut(s)	16, 53, 74, 80, 128
HpyCH4III	ACNGT	4 cut(s)	194, 1643, 1720, 1728
HpyCH4IV	ACGT	2 cut(s)	333, 994
HpyF10VI	GCNNNNNNNGC	7 cut(s)	212, 264, 989, 1064, 2036, 2045, 2243
HpySE526I	ACGT	2 cut(s)	333, 994
Hsp92I	GRCGYC	2 cut(s)	51, 126
HspAI	GCGC	2 cut(s)	102, 356
Kpn2I	TCCGGA	1 cut(s)	1051
KpnI	GGTACC	1 cut(s)	1622
Kzo9I	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
MabI	ACCWGGT	1 cut(s)	1468
MaeI	CTAG	6 cut(s)	44, 528, 1769, 1883, 2243, 2310
MaeII	ACGT	2 cut(s)	333, 994
MaeIII	GTNAC	4 cut(s)	523, 1282, 1728, 2334
MalI	GATC	6 cut(s)	430, 1041, 1116, 1382, 1963, 2192
MboI	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
MboII	GAAGA	9 cut(s)	423, 882, 999, 1122, 1186, 1597, 1622, 2377, 2387
MflI	RGATCY	2 cut(s)	428, 1961
MhlI	GDGCHC	2 cut(s)	1776, 1906
MlsI	TGGCCA	1 cut(s)	1451
MluNI	TGGCCA	1 cut(s)	1451
MlyI	GAGTC	7 cut(s)	74, 243, 1178, 1380, 1622, 2363, 2439
Mox20I	TGGCCA	1 cut(s)	1451
Mph1103I	ATGCAT	6 cut(s)	330, 781, 958, 1231, 1461, 2476
MroI	TCCGGA	1 cut(s)	1051
MroXI	GAANNNNTTC	2 cut(s)	1327, 1575
MscI	TGGCCA	1 cut(s)	1451
MseI	TTAA	5 cut(s)	408, 924, 1464, 2138, 2369
MslI	CAYNNNNRTG	2 cut(s)	2104, 2469
Msp20I	TGGCCA	1 cut(s)	1451
MspA1I	CMGCKG	2 cut(s)	501, 1349
MspI	CCGG	6 cut(s)	19, 240, 422, 724, 1052, 2071
MspR9I	CCNGG	8 cut(s)	20, 392, 423, 725, 830, 1470, 1787, 2157
Mva1269I	GAATGC	1 cut(s)	1558
MvaI	CCWGG	5 cut(s)	392, 830, 1470, 1787, 2157
MvnI	CGCG	1 cut(s)	356
MwoI	GCNNNNNNNGC	7 cut(s)	212, 264, 989, 1064, 2036, 2045, 2243
NciI	CCSGG	3 cut(s)	20, 423, 725
NcoI	CCATGG	1 cut(s)	1421
NdeI	CATATG	1 cut(s)	1657
NdeII	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
NlaIV	GGNNCC	3 cut(s)	1620, 1903, 1963
NmuCI	GTSAC	1 cut(s)	523
NsiI	ATGCAT	6 cut(s)	330, 781, 958, 1231, 1461, 2476
NspI	RCATGY	5 cut(s)	524, 779, 1459, 1736, 2151
PaeI	GCATGC	1 cut(s)	2151
PagI	TCATGA	1 cut(s)	1681
PceI	AGGCCT	2 cut(s)	206, 1502
PciI	ACATGT	1 cut(s)	520
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1558
PdmI	GAANNNNTTC	2 cut(s)	1327, 1575
PfeI	GAWTC	3 cut(s)	282, 373, 403
PflMI	CCANNNNNTGG	1 cut(s)	883
PleI	GAGTC	7 cut(s)	74, 242, 1178, 1380, 1622, 2362, 2438
PmaCI	CACGTG	1 cut(s)	995
PmlI	CACGTG	1 cut(s)	995
PpsI	GAGTC	7 cut(s)	74, 242, 1178, 1380, 1622, 2362, 2438
Ppu21I	YACGTR	1 cut(s)	995
PscI	ACATGT	1 cut(s)	520
Psp6I	CCWGG	5 cut(s)	390, 828, 1468, 1785, 2155
PspCI	CACGTG	1 cut(s)	995
PspEI	GGTNACC	1 cut(s)	2334
PspFI	CCCAGC	2 cut(s)	2026, 2109
PspGI	CCWGG	5 cut(s)	390, 828, 1468, 1785, 2155
PspN4I	GGNNCC	3 cut(s)	1620, 1903, 1963
PspPI	GGNCC	2 cut(s)	1343, 1830
PsuI	RGATCY	2 cut(s)	428, 1961
PvuII	CAGCTG	1 cut(s)	1349
RsaI	GTAC	3 cut(s)	517, 1620, 1911
RsaNI	GTAC	3 cut(s)	516, 1619, 1910
RseI	CAYNNNNRTG	2 cut(s)	2104, 2469
SalI	GTCGAC	2 cut(s)	47, 77
SaqAI	TTAA	5 cut(s)	408, 924, 1464, 2138, 2369
Sau3AI	GATC	6 cut(s)	428, 1039, 1114, 1380, 1961, 2190
Sau96I	GGNCC	2 cut(s)	1343, 1830
SchI	GAGTC	7 cut(s)	74, 243, 1178, 1380, 1622, 2363, 2439
ScrFI	CCNGG	8 cut(s)	20, 392, 423, 725, 830, 1470, 1787, 2157
SduI	GDGCHC	2 cut(s)	1776, 1906
SexAI	ACCWGGT	1 cut(s)	1468
SfcI	CTRYAG	1 cut(s)	1719
SinI	GGWCC	1 cut(s)	1343
SmiMI	CAYNNNNRTG	2 cut(s)	2104, 2469
SmlI	CTYRAG	2 cut(s)	697, 734
SmoI	CTYRAG	2 cut(s)	697, 734
SphI	GCATGC	1 cut(s)	2151
SseBI	AGGCCT	2 cut(s)	206, 1502
SspI	AATATT	2 cut(s)	1524, 1714
SspMI	CTAG	6 cut(s)	44, 528, 1769, 1883, 2243, 2310
StuI	AGGCCT	2 cut(s)	206, 1502
StyD4I	CCNGG	8 cut(s)	18, 390, 421, 723, 828, 1468, 1785, 2155
StyI	CCWWGG	3 cut(s)	207, 1421, 1983
TaaI	ACNGT	4 cut(s)	194, 1643, 1720, 1728
TaiI	ACGT	2 cut(s)	336, 997
TaqI	TCGA	4 cut(s)	48, 78, 606, 843
TatI	WGTACW	2 cut(s)	515, 1909
TauI	GCSGC	2 cut(s)	26, 762
TfiI	GAWTC	3 cut(s)	282, 373, 403
Tru1I	TTAA	5 cut(s)	408, 924, 1464, 2138, 2369
Tru9I	TTAA	5 cut(s)	408, 924, 1464, 2138, 2369
TscAI	CASTG	4 cut(s)	460, 877, 949, 1342
TseFI	GTSAC	1 cut(s)	523
Tsp45I	GTSAC	1 cut(s)	523
TspDTI	ATGAA	9 cut(s)	85, 707, 1548, 1565, 1584, 1670, 1698, 1936, 2386
TspGWI	ACGGA	3 cut(s)	1426, 1522, 1863
TspRI	CASTG	4 cut(s)	460, 877, 949, 1342
Van91I	CCANNNNNTGG	1 cut(s)	883
VpaK11BI	GGWCC	1 cut(s)	1343
XagI	CCTNNNNNAGG	1 cut(s)	1507
XapI	RAATTY	7 cut(s)	510, 1416, 1535, 1571, 1997, 2160, 2444
XceI	RCATGY	5 cut(s)	524, 779, 1459, 1736, 2151
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1476
XmiI	GTMKAC	4 cut(s)	48, 78, 837, 1591
XmnI	GAANNNNTTC	2 cut(s)	1327, 1575
XspI	CTAG	6 cut(s)	44, 528, 1769, 1883, 2243, 2310
Zsp2I	ATGCAT	6 cut(s)	330, 781, 958, 1231, 1461, 2476

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.