Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Forward (+)

33313504 .. 33321279

Gene Structure 7776 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024228

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGGATTTCACGGCGCTGCGGACGGCTGTGGAGGAGGCGGAGCTAGTCGACGCCCACGCCCACAACCTCGTCGCCACCGACTCCTCCTTCCCCTTCATCAGTGCCTTCTCGGAAGCCAACGCCGAGGCCTTATCCTACGCACCCCACTCCCTCTCCTTCAAGAGAAGTTTGAAGGAGGTTGCGGAGCTGTACGGGTGTGAGAAGTCCTTGGATGCGGTTGAGGAGCATCGGAGAGTCGCCGGATTGGAGGCGATTAGCTGCAGATGCTTTGAGGCTGCGAGAATCTCTGCGTTACTTATTGATGATGGACTGAGGCTGGACAAAATGCATGACATAGATTGGCACAAAAGTTTCGCGCCTGTGGTTGGGAGAATTCTGAGGATTGAGTCTCTGGCTGAGCAGATTCTTAGTGAGGAGTTGCCAAGTGGATCTTCTTGGACGTTGGATGTGTTCACTGAAGCATTTGTTGGAAAGTTGAATATAGCTGCATATCGCAGCGGTCTGGAAATTAGTACACATGTCACTAGGAAAGATGTTGAGGAAGGTCTTTCTGAGGTTATAAAGGCTGGAAAGCCTGTTCGCGTATCAAATAAAAGTTTTATCGACTATATTTTCACTCGTAGTTTGGAGGTTGCTCTACTTTTTGACTTGCCAATGCAGATACATACAGGTTTTGGCGACAAAGATTTGGACTTGAGGCTATCCAATCCCCTTCATCTCCGGGCTATGCTTGAGGACAAGAGATTTTCTAAAAGCCGTATTGTTCTTTTACATGCATCCTACCCGTTTTCAAAGGAAGCATCATATCTAGCCTCTGTTTATCCCCAGGTCTACCTCGACTTTGGCTTGGCTATACCCAAGCTCAGTGTCCACGGGATGGTATCTTCACTAAAAGAACTTTTGGAGCTTGCTCCAATTAAGAAGGTCATGTTCAGCACTGATGGATATGCATTTCCTGAAACTTTCTACTTAGGTGCAAAAAAGGCACGTGAAGTTGTCTTTTCAGTTCTATGTGATGCCTGTGCCGATGGTGATCTCTCAATTCCGGAAGCTATTGAGGCTGCTAAAGACATCTTTTCACAAAATGCAGTTCAGTTTTACAAGATTGATCGTGCTGTCAAATCTTCTGGTTCAGCCGATAGTGTATCCTCTAACTTTGTGAAGGTGAAGAGTAATGACTCGGAAAATCATGTCTCACTTGTTCGTGTTTTCTGGGCTGATGCATCAGGACAACAAAGATGTCGTGTTGTTCCTGGTAAGCGGTTCAATGACATAGCTACAAAAAATGGCATTGGTTTGACTTTTGCTTGTATGGGAATGACTTCTTTCGCTGATGGTCCAGCTGATGAGACTAATTTGACTGGAGTGGGTGAGATCAGACTCATGCCTGATTTATCGACTAAACGGAGAATTCCATGGGTAGAACAAGAGGAAATGGTTTTGGCCAACATGCATCTTAAACCAGGTGAAGCATGGGAATACTGCCCAAGAGAGGCCTTACGGAGGGTTTCAAAAATATTGAAAGATGAATTCAATTTGGTAATGAATGCAGGATTTGAAAATGAATTTTTTCTCTTGAAGAGTATACTAAGGGACGGGAAAGAAGAATGGGTACCATTTGACTCAACACCCTACTGTTCCCCATCATCGTATGATGCTGCTTCCACTTTGTTTCACGAAGTTCTTGCTGCTCTCCAGTCCTTGAATATTACTGTAGAACAGTTACATGCAGAAAGTGGGAAAGGTCAATTTGAGGTGGTTCTAGGGCACACAGCTTGCCAGCATGCTGCTGACAACTTGATTTATACCCGTGAAGTTATTAGGGCCATTGCAAGGAAACACGGATTGTTGGCAACCTTTGTCCCAAAGTATGCACTAGATGAGATTGGCTCTGGAGCCCATGTACATCTCAGTTTGTGGCAGAATGGCAAAAATGTATTCATGGCATCTGGTGGATCCTCTCAGCATGGAATGTCCAAGGTTGGGGAGGAATTTATGGCAGGGGTTTTATATCATCTCCCAGCAATTTTGGCATTTACAGCTCCAATTCCAAATAGTTACGACCGGATACAACCTAATACTTGGAGTGGAGCATACAAGTGCTGGGGAAAAGAAAACAGGGAAGCTCCATTAAGAACTGCATGTCCACCTGGAATTCAAGCTGGTCTGGTGAGCAACTTTGAGATCAAGTCATTTGATGGGTGTGCAAATCCACACTTGGGCTTGGCTGCAATACTAGCTGCTGGCATTGATGGTCTTAGGAGACATCTTTGTCTCCCTGAACCTATTGATACAAATCCTTCTAGCCTTGAAGGAGAACTACAAAGGTTACCAACTTCACTTTTGGAGTCATTAGAAGCTTTAAAGGAAGATGATCTCTTCAAAGAGTTCATTGGAGAAAAGCTATTGGTTGCCATAAAGGGAGTCCGCAAGGCAGAATTTGACTACTATGAGGAGCACAAAGATGCATACAAGCAACTTATACACCGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

832

Amino Acids

92.11

Weight (kDa)

5.68

Isoelectric Point (pI)

43.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_2	PF04909	215 - 368	8.6e-14	Amidohydrolase
Gln-synt_N_2	PF16952	388 - 473	1.2e-06	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	494 - 823	6.6e-91	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	560
AasI	GACNNNNNNGTC	1 cut(s)	2271
Acc65I	GGTACC	1 cut(s)	1612
AccB1I	GGYRCC	1 cut(s)	1612
AccB7I	CCANNNNNTGG	1 cut(s)	877
AccI	GTMKAC	3 cut(s)	48, 831, 1585
AccII	CGCG	2 cut(s)	356, 582
AccIII	TCCGGA	1 cut(s)	1045
AciI	CCGC	8 cut(s)	19, 38, 182, 215, 498, 1261, 2428, 2488
AclWI	GGATC	3 cut(s)	436, 1950, 1963
AcoI	YGGCCR	1 cut(s)	1443
AcsI	RAATTY	7 cut(s)	372, 1410, 1529, 1565, 1991, 2154, 2438
AcuI	CTGAAG	1 cut(s)	477
AcvI	CACGTG	1 cut(s)	989
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	4 cut(s)	191, 514, 1614, 1905
AfiI	CCNNNNNNNGG	7 cut(s)	365, 877, 1492, 1503, 1833, 1983, 2219
AflIII	ACRYGT	1 cut(s)	517
AjnI	CCWGG	4 cut(s)	825, 1252, 1462, 2149
AjuI	GAANNNNNNNTTGG	4 cut(s)	415, 447, 450, 482
AloI	GAACNNNNNNTCC	4 cut(s)	561, 593, 2128, 2160
Alw21I	GWGCWC	1 cut(s)	2460
Alw26I	GTCTC	5 cut(s)	393, 1198, 1343, 2257, 2279
AlwI	GGATC	3 cut(s)	436, 1950, 1963
Aor13HI	TCCGGA	1 cut(s)	1045
AoxI	GGCC	4 cut(s)	126, 1443, 1494, 1824
ApoI	RAATTY	7 cut(s)	372, 1410, 1529, 1565, 1991, 2154, 2438
Asp700I	GAANNNNTTC	2 cut(s)	1321, 1569
Asp718I	GGTACC	1 cut(s)	1612
AspLEI	GCGC	2 cut(s)	16, 358
AspS9I	GGNCC	2 cut(s)	1337, 1824
AsuC2I	CCSGG	1 cut(s)	722
AsuHPI	GGTGA	5 cut(s)	1043, 1177, 1382, 1478, 2182
AvaII	GGWCC	1 cut(s)	1337
BaeGI	GKGCMC	1 cut(s)	1770
BalI	TGGCCA	1 cut(s)	1445
BamHI	GGATCC	1 cut(s)	1955
BanI	GGYRCC	1 cut(s)	1612
BanII	GRGCYC	1 cut(s)	1900
BarI	GAAGNNNNNNTAC	4 cut(s)	1596, 1628, 2304, 2336
BbrPI	CACGTG	1 cut(s)	989
Bbv12I	GWGCWC	1 cut(s)	2460
BccI	CCATC	8 cut(s)	299, 871, 935, 1022, 1328, 1651, 2192, 2246
BceAI	ACGGC	3 cut(s)	27, 39, 741
BcgI	CGANNNNNNTGC	2 cut(s)	1667, 1701
BciT130I	CCWGG	4 cut(s)	827, 1254, 1464, 2151
BciVI	GTATCC	2 cut(s)	1156, 2061
BcnI	CCSGG	1 cut(s)	722
BcoDI	GTCTC	5 cut(s)	393, 1198, 1343, 2257, 2279
BfaI	CTAG	7 cut(s)	44, 525, 809, 1763, 1877, 2237, 2304
BfmI	CTRYAG	2 cut(s)	259, 1713
BfoI	RGCGCY	1 cut(s)	17
BfuI	GTATCC	2 cut(s)	1156, 2061
BlpI	GCTNAGC	1 cut(s)	396
Bme1390I	CCNGG	5 cut(s)	722, 827, 1254, 1464, 2151
Bme18I	GGWCC	1 cut(s)	1337
BmgT120I	GGNCC	2 cut(s)	1337, 1824
BmiI	GGNNCC	3 cut(s)	1614, 1897, 1957
BmrFI	CCNGG	5 cut(s)	722, 827, 1254, 1464, 2151
BplI	GAGNNNNNCTC	2 cut(s)	1874, 1906
BpmI	CTGGAG	3 cut(s)	1383, 1679, 1914
Bpu1102I	GCTNAGC	1 cut(s)	396
BpuEI	CTTGAG	2 cut(s)	715, 752
BpuMI	CCSGG	1 cut(s)	722
BsaAI	YACGTR	1 cut(s)	989
BsaBI	GATNNNNATC	2 cut(s)	1032, 2295
BsaHI	GRCGYC	1 cut(s)	51
BsaJI	CCNNGG	6 cut(s)	123, 207, 825, 871, 1415, 1977
BsaWI	WCCGGW	2 cut(s)	1045, 2064
BsaXI	ACNNNNNCTCC	4 cut(s)	137, 167, 1887, 1917
Bsc4I	CCNNNNNNNGG	7 cut(s)	365, 877, 1492, 1503, 1833, 1983, 2219
Bse1I	ACTGG	2 cut(s)	1366, 1696
Bse3DI	GCAATG	1 cut(s)	1827
Bse8I	GATNNNNATC	2 cut(s)	1032, 2295
BseAI	TCCGGA	1 cut(s)	1045
BseBI	CCWGG	4 cut(s)	827, 1254, 1464, 2151
BseDI	CCNNGG	6 cut(s)	123, 207, 825, 871, 1415, 1977
BseGI	GGATG	4 cut(s)	217, 451, 776, 882
BseJI	GATNNNNATC	2 cut(s)	1032, 2295
BseLI	CCNNNNNNNGG	7 cut(s)	365, 877, 1492, 1503, 1833, 1983, 2219
BseMI	GCAATG	1 cut(s)	1827
BseMII	CTCAG	7 cut(s)	302, 368, 387, 543, 877, 1924, 1976
BseNI	ACTGG	2 cut(s)	1366, 1696
BseRI	GAGGAG	5 cut(s)	47, 73, 236, 428, 2468
BseSI	GKGCMC	1 cut(s)	1770
BseYI	CCCAGC	2 cut(s)	2020, 2103
Bsh1236I	CGCG	2 cut(s)	356, 582
Bsh1285I	CGRYCG	1 cut(s)	2065
BshFI	GGCC	4 cut(s)	128, 1445, 1496, 1826
BshNI	GGYRCC	1 cut(s)	1612
BsiEI	CGRYCG	1 cut(s)	2065
BsiHKAI	GWGCWC	1 cut(s)	2460
BsiSI	CCGG	4 cut(s)	240, 721, 1046, 2065
BslFI	GGGAC	2 cut(s)	1607, 1847
BslI	CCNNNNNNNGG	7 cut(s)	365, 877, 1492, 1503, 1833, 1983, 2219
BsmAI	GTCTC	5 cut(s)	393, 1198, 1343, 2257, 2279
BsmFI	GGGAC	2 cut(s)	1607, 1847
BsmI	GAATGC	1 cut(s)	1552
BsnI	GGCC	4 cut(s)	128, 1445, 1496, 1826
Bsp1286I	GDGCHC	3 cut(s)	1770, 1900, 2460
Bsp13I	TCCGGA	1 cut(s)	1045
Bsp1407I	TGTACA	1 cut(s)	1903
Bsp143I	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
Bsp1720I	GCTNAGC	1 cut(s)	396
Bsp19I	CCATGG	1 cut(s)	1415
BspACI	CCGC	8 cut(s)	19, 38, 182, 215, 498, 1261, 2428, 2488
BspANI	GGCC	4 cut(s)	128, 1445, 1496, 1826
BspCNI	CTCAG	7 cut(s)	303, 369, 388, 544, 876, 1923, 1975
BspEI	TCCGGA	1 cut(s)	1045
BspFNI	CGCG	2 cut(s)	356, 582
BspLI	GGNNCC	3 cut(s)	1614, 1897, 1957
BspMAI	CTGCAG	1 cut(s)	263
BspPI	GGATC	3 cut(s)	436, 1950, 1963
BspT107I	GGYRCC	1 cut(s)	1612
BsrDI	GCAATG	1 cut(s)	1827
BsrGI	TGTACA	1 cut(s)	1903
BsrI	ACTGG	2 cut(s)	1366, 1696
BssECI	CCNNGG	6 cut(s)	123, 207, 825, 871, 1415, 1977
BssMI	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
BssNAI	GTATAC	1 cut(s)	1586
BssNI	GRCGYC	1 cut(s)	51
BssT1I	CCWWGG	3 cut(s)	207, 1415, 1977
Bst1107I	GTATAC	1 cut(s)	1586
Bst2UI	CCWGG	4 cut(s)	827, 1254, 1464, 2151
Bst4CI	ACNGT	3 cut(s)	1637, 1714, 1722
Bst6I	CTCTTC	3 cut(s)	1163, 1574, 2384
BstACI	GRCGYC	1 cut(s)	51
BstAUI	TGTACA	1 cut(s)	1903
BstBAI	YACGTR	1 cut(s)	989
BstC8I	GCNNGC	5 cut(s)	909, 1777, 1781, 1785, 2245
BstDSI	CCRYGG	2 cut(s)	871, 1415
BstEII	GGTNACC	1 cut(s)	2328
BstENI	CCTNNNNNAGG	1 cut(s)	1501
BstF5I	GGATG	4 cut(s)	217, 451, 776, 882
BstFNI	CGCG	2 cut(s)	356, 582
BstH2I	RGCGCY	1 cut(s)	17
BstHHI	GCGC	2 cut(s)	16, 358
BstKTI	GATC	7 cut(s)	431, 1036, 1111, 1377, 1958, 2187, 2377
BstMAI	GTCTC	5 cut(s)	393, 1198, 1343, 2257, 2279
BstMBI	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
BstMCI	CGRYCG	1 cut(s)	2065
BstMWI	GCNNNNNNNGC	6 cut(s)	264, 983, 1058, 2030, 2039, 2237
BstNI	CCWGG	4 cut(s)	827, 1254, 1464, 2151
BstNSI	RCATGY	6 cut(s)	521, 776, 1453, 1730, 1787, 2145
BstPI	GGTNACC	1 cut(s)	2328
BstSCI	CCNGG	5 cut(s)	720, 825, 1252, 1462, 2149
BstSFI	CTRYAG	2 cut(s)	259, 1713
BstSLI	GKGCMC	1 cut(s)	1770
BstUI	CGCG	2 cut(s)	356, 582
BstX2I	RGATCY	2 cut(s)	428, 1955
BstYI	RGATCY	2 cut(s)	428, 1955
BstZ17I	GTATAC	1 cut(s)	1586
BsuI	GTATCC	2 cut(s)	1156, 2061
BsuRI	GGCC	4 cut(s)	128, 1445, 1496, 1826
BtgI	CCRYGG	2 cut(s)	871, 1415
BtsCI	GGATG	4 cut(s)	217, 451, 776, 882
BtsIMutI	CAGTG	4 cut(s)	106, 453, 871, 936
Cac8I	GCNNGC	5 cut(s)	909, 1777, 1781, 1785, 2245
CfoI	GCGC	2 cut(s)	16, 358
Cfr13I	GGNCC	2 cut(s)	1337, 1824
CseI	GACGC	1 cut(s)	59
CsiI	ACCWGGT	1 cut(s)	1462
Csp6I	GTAC	4 cut(s)	190, 513, 1613, 1904
CviQI	GTAC	4 cut(s)	190, 513, 1613, 1904
DpnI	GATC	7 cut(s)	430, 1035, 1110, 1376, 1957, 2186, 2376
DpnII	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
DraI	TTTAAA	1 cut(s)	2364
DrdI	GACNNNNNNGTC	1 cut(s)	2271
DseDI	GACNNNNNNGTC	1 cut(s)	2271
EaeI	YGGCCR	1 cut(s)	1443
Eam1104I	CTCTTC	3 cut(s)	1163, 1574, 2384
EarI	CTCTTC	3 cut(s)	1163, 1574, 2384
EciI	GGCGGA	1 cut(s)	53
Eco130I	CCWWGG	3 cut(s)	207, 1415, 1977
Eco147I	AGGCCT	2 cut(s)	128, 1496
Eco24I	GRGCYC	1 cut(s)	1900
Eco47I	GGWCC	1 cut(s)	1337
Eco57I	CTGAAG	1 cut(s)	477
Eco72I	CACGTG	1 cut(s)	989
Eco91I	GGTNACC	1 cut(s)	2328
EcoNI	CCTNNNNNAGG	1 cut(s)	1501
EcoO65I	GGTNACC	1 cut(s)	2328
EcoRI	GAATTC	4 cut(s)	372, 1410, 1529, 2154
EcoRII	CCWGG	4 cut(s)	825, 1252, 1462, 2149
EcoT14I	CCWWGG	3 cut(s)	207, 1415, 1977
EcoT22I	ATGCAT	6 cut(s)	330, 778, 952, 1225, 1455, 2470
EcoT38I	GRGCYC	1 cut(s)	1900
ErhI	CCWWGG	3 cut(s)	207, 1415, 1977
FalI	AAGNNNNNCTT	2 cut(s)	415, 447
FaqI	GGGAC	2 cut(s)	1607, 1847
FblI	GTMKAC	3 cut(s)	48, 831, 1585
FokI	GGATG	4 cut(s)	224, 458, 763, 889
FriOI	GRGCYC	1 cut(s)	1900
FspBI	CTAG	7 cut(s)	44, 525, 809, 1763, 1877, 2237, 2304
GlaI	GCGC	2 cut(s)	15, 357
GsaI	CCCAGC	2 cut(s)	2024, 2107
GsuI	CTGGAG	3 cut(s)	1383, 1679, 1914
HaeII	RGCGCY	1 cut(s)	17
HaeIII	GGCC	4 cut(s)	128, 1445, 1496, 1826
HapII	CCGG	4 cut(s)	240, 721, 1046, 2065
HgaI	GACGC	1 cut(s)	59
HhaI	GCGC	2 cut(s)	16, 358
Hin1I	GRCGYC	1 cut(s)	51
Hin6I	GCGC	2 cut(s)	14, 356
HinP1I	GCGC	2 cut(s)	14, 356
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	1 cut(s)	2358
HpaII	CCGG	4 cut(s)	240, 721, 1046, 2065
HphI	GGTGA	5 cut(s)	1043, 1177, 1382, 1478, 2182
Hpy166II	GTNNAC	7 cut(s)	49, 453, 515, 832, 871, 1586, 2147
Hpy188I	TCNGA	6 cut(s)	112, 231, 378, 553, 1183, 1379
Hpy188III	TCNNGA	8 cut(s)	160, 503, 956, 1046, 1227, 1576, 1676, 1893
Hpy8I	GTNNAC	7 cut(s)	49, 453, 515, 832, 871, 1586, 2147
Hpy99I	CGWCG	2 cut(s)	53, 74
HpyCH4III	ACNGT	3 cut(s)	1637, 1714, 1722
HpyCH4IV	ACGT	2 cut(s)	440, 988
HpyF10VI	GCNNNNNNNGC	6 cut(s)	264, 983, 1058, 2030, 2039, 2237
HpySE526I	ACGT	2 cut(s)	440, 988
Hsp92I	GRCGYC	1 cut(s)	51
HspAI	GCGC	2 cut(s)	14, 356
Kpn2I	TCCGGA	1 cut(s)	1045
KpnI	GGTACC	1 cut(s)	1616
Kzo9I	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
MabI	ACCWGGT	1 cut(s)	1462
MaeI	CTAG	7 cut(s)	44, 525, 809, 1763, 1877, 2237, 2304
MaeII	ACGT	2 cut(s)	440, 988
MaeIII	GTNAC	5 cut(s)	291, 520, 1722, 2057, 2328
MalI	GATC	7 cut(s)	430, 1035, 1110, 1376, 1957, 2186, 2376
MboI	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
MboII	GAAGA	8 cut(s)	423, 876, 1116, 1180, 1591, 1616, 2371, 2381
MflI	RGATCY	2 cut(s)	428, 1955
MhlI	GDGCHC	3 cut(s)	1770, 1900, 2460
MlsI	TGGCCA	1 cut(s)	1445
MluNI	TGGCCA	1 cut(s)	1445
MlyI	GAGTC	8 cut(s)	74, 243, 395, 1172, 1374, 1616, 2357, 2433
MmeI	TCCRAC	2 cut(s)	423, 448
Mox20I	TGGCCA	1 cut(s)	1445
Mph1103I	ATGCAT	6 cut(s)	330, 778, 952, 1225, 1455, 2470
MroI	TCCGGA	1 cut(s)	1045
MroXI	GAANNNNTTC	2 cut(s)	1321, 1569
MscI	TGGCCA	1 cut(s)	1445
MseI	TTAA	4 cut(s)	918, 1458, 2132, 2363
MslI	CAYNNNNRTG	2 cut(s)	2098, 2463
Msp20I	TGGCCA	1 cut(s)	1445
MspA1I	CMGCKG	2 cut(s)	498, 1343
MspI	CCGG	4 cut(s)	240, 721, 1046, 2065
MspR9I	CCNGG	5 cut(s)	722, 827, 1254, 1464, 2151
Mva1269I	GAATGC	1 cut(s)	1552
MvaI	CCWGG	4 cut(s)	827, 1254, 1464, 2151
MvnI	CGCG	2 cut(s)	356, 582
MwoI	GCNNNNNNNGC	6 cut(s)	264, 983, 1058, 2030, 2039, 2237
NciI	CCSGG	1 cut(s)	722
NcoI	CCATGG	1 cut(s)	1415
NdeII	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
NlaIV	GGNNCC	3 cut(s)	1614, 1897, 1957
NmeAIII	GCCGAG	1 cut(s)	148
NmuCI	GTSAC	1 cut(s)	520
NsiI	ATGCAT	6 cut(s)	330, 778, 952, 1225, 1455, 2470
NspI	RCATGY	6 cut(s)	521, 776, 1453, 1730, 1787, 2145
PaeI	GCATGC	1 cut(s)	1787
PceI	AGGCCT	2 cut(s)	128, 1496
PciI	ACATGT	1 cut(s)	517
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1552
PdmI	GAANNNNTTC	2 cut(s)	1321, 1569
PfeI	GAWTC	2 cut(s)	282, 403
PflMI	CCANNNNNTGG	1 cut(s)	877
PleI	GAGTC	8 cut(s)	74, 242, 394, 1172, 1374, 1616, 2356, 2432
PmaCI	CACGTG	1 cut(s)	989
PmlI	CACGTG	1 cut(s)	989
PpsI	GAGTC	8 cut(s)	74, 242, 394, 1172, 1374, 1616, 2356, 2432
Ppu21I	YACGTR	1 cut(s)	989
PscI	ACATGT	1 cut(s)	517
PsiI	TTATAA	1 cut(s)	560
Psp6I	CCWGG	4 cut(s)	825, 1252, 1462, 2149
PspCI	CACGTG	1 cut(s)	989
PspEI	GGTNACC	1 cut(s)	2328
PspFI	CCCAGC	2 cut(s)	2020, 2103
PspGI	CCWGG	4 cut(s)	825, 1252, 1462, 2149
PspN4I	GGNNCC	3 cut(s)	1614, 1897, 1957
PspPI	GGNCC	2 cut(s)	1337, 1824
PstI	CTGCAG	1 cut(s)	263
PsuI	RGATCY	2 cut(s)	428, 1955
PvuII	CAGCTG	1 cut(s)	1343
RsaI	GTAC	4 cut(s)	191, 514, 1614, 1905
RsaNI	GTAC	4 cut(s)	190, 513, 1613, 1904
RseI	CAYNNNNRTG	2 cut(s)	2098, 2463
SalI	GTCGAC	1 cut(s)	47
SaqAI	TTAA	4 cut(s)	918, 1458, 2132, 2363
Sau3AI	GATC	7 cut(s)	428, 1033, 1108, 1374, 1955, 2184, 2374
Sau96I	GGNCC	2 cut(s)	1337, 1824
SchI	GAGTC	8 cut(s)	74, 243, 395, 1172, 1374, 1616, 2357, 2433
ScrFI	CCNGG	5 cut(s)	722, 827, 1254, 1464, 2151
SduI	GDGCHC	3 cut(s)	1770, 1900, 2460
SexAI	ACCWGGT	1 cut(s)	1462
SfcI	CTRYAG	2 cut(s)	259, 1713
SinI	GGWCC	1 cut(s)	1337
SmiMI	CAYNNNNRTG	2 cut(s)	2098, 2463
SmlI	CTYRAG	2 cut(s)	694, 731
SmoI	CTYRAG	2 cut(s)	694, 731
SphI	GCATGC	1 cut(s)	1787
SseBI	AGGCCT	2 cut(s)	128, 1496
SsiI	CCGC	8 cut(s)	19, 38, 182, 215, 498, 1261, 2428, 2488
SspI	AATATT	2 cut(s)	1518, 1708
SspMI	CTAG	7 cut(s)	44, 525, 809, 1763, 1877, 2237, 2304
StuI	AGGCCT	2 cut(s)	128, 1496
StyD4I	CCNGG	5 cut(s)	720, 825, 1252, 1462, 2149
StyI	CCWWGG	3 cut(s)	207, 1415, 1977
TaaI	ACNGT	3 cut(s)	1637, 1714, 1722
TaiI	ACGT	2 cut(s)	443, 991
TaqI	TCGA	4 cut(s)	48, 603, 837, 1397
TatI	WGTACW	2 cut(s)	512, 1903
TfiI	GAWTC	2 cut(s)	282, 403
Tru1I	TTAA	4 cut(s)	918, 1458, 2132, 2363
Tru9I	TTAA	4 cut(s)	918, 1458, 2132, 2363
TscAI	CASTG	4 cut(s)	106, 460, 871, 943
TseFI	GTSAC	1 cut(s)	520
Tsp45I	GTSAC	1 cut(s)	520
TspDTI	ATGAA	7 cut(s)	85, 704, 1542, 1559, 1578, 1930, 2380
TspGWI	ACGGA	3 cut(s)	1420, 1516, 1857
TspRI	CASTG	4 cut(s)	106, 460, 871, 943
Van91I	CCANNNNNTGG	1 cut(s)	877
VpaK11BI	GGWCC	1 cut(s)	1337
XagI	CCTNNNNNAGG	1 cut(s)	1501
XapI	RAATTY	7 cut(s)	372, 1410, 1529, 1565, 1991, 2154, 2438
XceI	RCATGY	6 cut(s)	521, 776, 1453, 1730, 1787, 2145
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1470
XmiI	GTMKAC	3 cut(s)	48, 831, 1585
XmnI	GAANNNNTTC	2 cut(s)	1321, 1569
XspI	CTAG	7 cut(s)	44, 525, 809, 1763, 1877, 2237, 2304
Zsp2I	ATGCAT	6 cut(s)	330, 778, 952, 1225, 1455, 2470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.