Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Reverse (-)

51397088 .. 51400087

Gene Structure 3000 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1214800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 741 bp

ATGGCACTTCGGCACACTGCTTGTATGCATGCTGCTGACAACCTGATTTATACTCGTGAAGTTATTAGAGCCATTGCAAGGAAACATGGATTGTTGGCAACTTTCATGCCAAAGTATGCTCTTGATGACATTGGCTCTGGGGCGCATGTACATCTTAGTTTGTGGCAGAATGGCACAAATGTGTTTACGGCATCTGGTGGTTCCTCTCAGCATGGAATGTCAAAGATCGGGGAAGAATTCATGGCAGGGGTTTTACACCATCTTCCTGCAATTTTGGCATTTATAGCACCGATTCCAAATAGTTATGACCGAATTCAACCTAATACATGGAGTGGAGCATACAAGTGTTGGGGAAAAGAAAACAGAGAAGCTCCGTTAAGAACTGCATGCCCACCTGGAATACAAGCTGGTTTGGTGAGCAACTTTGAGATTAAATCATTTGATGGATGTGCAAATCCACACTTAGGCTTGGCTGCAATACTTGCTGGTGGTATTGATGGTCTTAGGAATCATCTTCGTCTCCCGGAACCTGTTGATACAAATCCTTCTGGCCTTGGTGCAGAAGTAGAAAGGTTGCCGAAATCACTTTCTGAATCTTTAGAAGCTCTCAAGGAAGACAATTTGTTCGCAGATTTACTTGGGGAAAACCTATTGGTTGCGATAAAAGGGGTCCGCAAGGCGGAGATTGACTACTACTCGAAGAACAAAGATGCATACAAGCAACTTATATACCGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

247

Amino Acids

26.86

Weight (kDa)

7.15

Isoelectric Point (pI)

38.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gln-synt_C	PF00120	3 - 239	1.9e-72	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	673, 680, 733
AcsI	RAATTY	2 cut(s)	236, 312
AfaI	GTAC	1 cut(s)	150
AfiI	CCNNNNNNNGG	3 cut(s)	78, 464, 679
AgsI	TTSAA	1 cut(s)	317
AjnI	CCWGG	1 cut(s)	394
AleI	CACNNNNGTG	1 cut(s)	179
AluBI	AGCT	3 cut(s)	371, 407, 605
AluI	AGCT	3 cut(s)	371, 407, 605
Alw26I	GTCTC	1 cut(s)	524
AoxI	GGCC	1 cut(s)	550
ApeKI	GCWGC	2 cut(s)	32, 473
ApoI	RAATTY	2 cut(s)	236, 312
ArsI	GACNNNNNNTTYG	2 cut(s)	608, 640
AspLEI	GCGC	1 cut(s)	145
AspS9I	GGNCC	1 cut(s)	670
AsuC2I	CCSGG	1 cut(s)	524
AsuHPI	GGTGA	1 cut(s)	427
AvaII	GGWCC	1 cut(s)	670
BauI	CACGAG	1 cut(s)	54
BbsI	GAAGAC	1 cut(s)	621
BbvI	GCAGC	2 cut(s)	19, 460
BccI	CCATC	3 cut(s)	267, 437, 491
BceAI	ACGGC	1 cut(s)	204
BciT130I	CCWGG	1 cut(s)	396
BcnI	CCSGG	1 cut(s)	524
BcoDI	GTCTC	1 cut(s)	524
BisI	GCNGC	2 cut(s)	33, 474
BlsI	GCNGC	2 cut(s)	34, 475
Bme1390I	CCNGG	2 cut(s)	396, 524
Bme18I	GGWCC	1 cut(s)	670
BmgT120I	GGNCC	1 cut(s)	670
BmiI	GGNNCC	3 cut(s)	202, 528, 671
BmrFI	CCNGG	2 cut(s)	396, 524
BmsI	GCATC	2 cut(s)	200, 700
BpiI	GAAGAC	1 cut(s)	621
BpuEI	CTTGAG	1 cut(s)	593
BpuMI	CCSGG	1 cut(s)	524
BsaBI	GATNNNNATC	1 cut(s)	540
BsaJI	CCNNGG	1 cut(s)	553
Bsc4I	CCNNNNNNNGG	3 cut(s)	78, 464, 679
Bse3DI	GCAATG	1 cut(s)	72
Bse8I	GATNNNNATC	1 cut(s)	540
BseBI	CCWGG	1 cut(s)	396
BseDI	CCNNGG	1 cut(s)	553
BseGI	GGATG	1 cut(s)	452
BseJI	GATNNNNATC	1 cut(s)	540
BseLI	CCNNNNNNNGG	3 cut(s)	78, 464, 679
BseMI	GCAATG	1 cut(s)	72
BseMII	CTCAG	1 cut(s)	221
BseXI	GCAGC	2 cut(s)	19, 460
BsgI	GTGCAG	1 cut(s)	579
BshFI	GGCC	1 cut(s)	552
BsiSI	CCGG	1 cut(s)	524
BslI	CCNNNNNNNGG	3 cut(s)	78, 464, 679
BsmAI	GTCTC	1 cut(s)	524
BsmBI	CGTCTC	1 cut(s)	524
BsnI	GGCC	1 cut(s)	552
Bsp1407I	TGTACA	1 cut(s)	148
Bsp143I	GATC	1 cut(s)	225
BspACI	CCGC	3 cut(s)	673, 680, 733
BspANI	GGCC	1 cut(s)	552
BspCNI	CTCAG	1 cut(s)	220
BspLI	GGNNCC	3 cut(s)	202, 528, 671
BsrDI	GCAATG	1 cut(s)	72
BsrGI	TGTACA	1 cut(s)	148
BssECI	CCNNGG	1 cut(s)	553
BssMI	GATC	1 cut(s)	225
BssSI	CACGAG	1 cut(s)	54
BssT1I	CCWWGG	1 cut(s)	553
Bst2BI	CACGAG	1 cut(s)	54
Bst2UI	CCWGG	1 cut(s)	396
BstAPI	GCANNNNNTGC	1 cut(s)	482
BstAUI	TGTACA	1 cut(s)	148
BstC8I	GCNNGC	2 cut(s)	30, 388
BstDEI	CTNAG	4 cut(s)	155, 207, 463, 503
BstF5I	GGATG	1 cut(s)	452
BstHHI	GCGC	1 cut(s)	145
BstKTI	GATC	1 cut(s)	228
BstMAI	GTCTC	1 cut(s)	524
BstMBI	GATC	1 cut(s)	225
BstMWI	GCNNNNNNNGC	3 cut(s)	275, 284, 482
BstNI	CCWGG	1 cut(s)	396
BstNSI	RCATGY	3 cut(s)	32, 149, 390
BstSCI	CCNGG	2 cut(s)	394, 522
BstV1I	GCAGC	2 cut(s)	19, 460
BstV2I	GAAGAC	1 cut(s)	621
BsuRI	GGCC	1 cut(s)	552
BtsCI	GGATG	1 cut(s)	452
BtsI	GCAGTG	1 cut(s)	15
BtsIMutI	CAGTG	1 cut(s)	15
Cac8I	GCNNGC	2 cut(s)	30, 388
CfoI	GCGC	1 cut(s)	145
Cfr13I	GGNCC	1 cut(s)	670
Csp6I	GTAC	1 cut(s)	149
CviAII	CATG	8 cut(s)	29, 86, 106, 146, 212, 241, 327, 387
CviJI	RGCY	8 cut(s)	71, 135, 371, 407, 468, 473, 552, 605
CviKI_1	RGCY	8 cut(s)	71, 135, 371, 407, 468, 473, 552, 605
CviQI	GTAC	1 cut(s)	149
DdeI	CTNAG	4 cut(s)	155, 207, 463, 503
DpnI	GATC	1 cut(s)	227
DpnII	GATC	1 cut(s)	225
EciI	GGCGGA	1 cut(s)	695
Eco130I	CCWWGG	1 cut(s)	553
Eco47I	GGWCC	1 cut(s)	670
EcoRI	GAATTC	2 cut(s)	236, 312
EcoRII	CCWGG	1 cut(s)	394
EcoT14I	CCWWGG	1 cut(s)	553
EcoT22I	ATGCAT	2 cut(s)	30, 715
ErhI	CCWWGG	1 cut(s)	553
Esp3I	CGTCTC	1 cut(s)	524
FaeI	CATG	8 cut(s)	32, 89, 109, 149, 215, 244, 330, 390
FatI	CATG	8 cut(s)	28, 85, 105, 145, 211, 240, 326, 386
Fnu4HI	GCNGC	2 cut(s)	33, 474
FokI	GGATG	1 cut(s)	459
Fsp4HI	GCNGC	2 cut(s)	33, 474
GlaI	GCGC	1 cut(s)	144
GluI	GCNGC	2 cut(s)	33, 474
HaeIII	GGCC	1 cut(s)	552
HapII	CCGG	1 cut(s)	524
HhaI	GCGC	1 cut(s)	145
Hin1II	CATG	8 cut(s)	32, 89, 109, 149, 215, 244, 330, 390
Hin6I	GCGC	1 cut(s)	143
HinP1I	GCGC	1 cut(s)	143
HinfI	GANTC	3 cut(s)	292, 508, 593
HpaII	CCGG	1 cut(s)	524
HphI	GGTGA	1 cut(s)	427
Hpy166II	GTNNAC	1 cut(s)	186
Hpy188I	TCNGA	1 cut(s)	592
Hpy188III	TCNNGA	2 cut(s)	56, 122
Hpy8I	GTNNAC	1 cut(s)	186
HpyAV	CCTTC	1 cut(s)	555
HpyCH4V	TGCA	8 cut(s)	28, 77, 269, 386, 452, 476, 560, 713
HpyF10VI	GCNNNNNNNGC	3 cut(s)	275, 284, 482
HpyF3I	CTNAG	4 cut(s)	155, 207, 463, 503
Hsp92II	CATG	8 cut(s)	32, 89, 109, 149, 215, 244, 330, 390
HspAI	GCGC	1 cut(s)	143
Kzo9I	GATC	1 cut(s)	225
LmnI	GCTCC	2 cut(s)	335, 376
Lsp1109I	GCAGC	2 cut(s)	19, 460
LweI	GCATC	2 cut(s)	200, 700
MalI	GATC	1 cut(s)	227
MboI	GATC	1 cut(s)	225
MboII	GAAGA	5 cut(s)	245, 254, 506, 626, 712
MluCI	AATT	4 cut(s)	236, 270, 312, 619
MnlI	CCTC	1 cut(s)	214
Mph1103I	ATGCAT	2 cut(s)	30, 715
MseI	TTAA	2 cut(s)	377, 432
MslI	CAYNNNNRTG	2 cut(s)	179, 343
MspI	CCGG	1 cut(s)	524
MspR9I	CCNGG	2 cut(s)	396, 524
MvaI	CCWGG	1 cut(s)	396
MwoI	GCNNNNNNNGC	3 cut(s)	275, 284, 482
NciI	CCSGG	1 cut(s)	524
NdeII	GATC	1 cut(s)	225
NlaIII	CATG	8 cut(s)	32, 89, 109, 149, 215, 244, 330, 390
NlaIV	GGNNCC	3 cut(s)	202, 528, 671
NsiI	ATGCAT	2 cut(s)	30, 715
NspI	RCATGY	3 cut(s)	32, 149, 390
OliI	CACNNNNGTG	1 cut(s)	179
PaeI	GCATGC	2 cut(s)	32, 390
PfeI	GAWTC	3 cut(s)	292, 508, 593
PfoI	TCCNGGA	1 cut(s)	522
PkrI	GCNGC	2 cut(s)	34, 475
Psp6I	CCWGG	1 cut(s)	394
PspGI	CCWGG	1 cut(s)	394
PspN4I	GGNNCC	3 cut(s)	202, 528, 671
PspPI	GGNCC	1 cut(s)	670
RsaI	GTAC	1 cut(s)	150
RsaNI	GTAC	1 cut(s)	149
RseI	CAYNNNNRTG	2 cut(s)	179, 343
SaqAI	TTAA	2 cut(s)	377, 432
SatI	GCNGC	2 cut(s)	33, 474
Sau3AI	GATC	1 cut(s)	225
Sau96I	GGNCC	1 cut(s)	670
ScrFI	CCNGG	2 cut(s)	396, 524
SetI	ASST	9 cut(s)	45, 322, 373, 397, 409, 532, 575, 607, 651
SfaNI	GCATC	2 cut(s)	200, 700
SinI	GGWCC	1 cut(s)	670
SmiMI	CAYNNNNRTG	2 cut(s)	179, 343
SmlI	CTYRAG	1 cut(s)	608
SmoI	CTYRAG	1 cut(s)	608
SphI	GCATGC	2 cut(s)	32, 390
Sse9I	AATT	4 cut(s)	236, 270, 312, 619
SsiI	CCGC	3 cut(s)	673, 680, 733
StyD4I	CCNGG	2 cut(s)	394, 522
StyI	CCWWGG	1 cut(s)	553
TaqI	TCGA	1 cut(s)	698
TaqII	GACCGA	1 cut(s)	324
TasI	AATT	4 cut(s)	236, 270, 312, 619
TatI	WGTACW	1 cut(s)	148
TfiI	GAWTC	3 cut(s)	292, 508, 593
Tru1I	TTAA	2 cut(s)	377, 432
Tru9I	TTAA	2 cut(s)	377, 432
TscAI	CASTG	1 cut(s)	22
TseI	GCWGC	2 cut(s)	32, 473
TspDTI	ATGAA	2 cut(s)	94, 229
TspGWI	ACGGA	1 cut(s)	363
TspRI	CASTG	1 cut(s)	22
VpaK11BI	GGWCC	1 cut(s)	670
XapI	RAATTY	2 cut(s)	236, 312
XceI	RCATGY	3 cut(s)	32, 149, 390
Zsp2I	ATGCAT	2 cut(s)	30, 715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.