Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Reverse (-)

13576961 .. 13584077

Gene Structure 7117 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G011740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2565 bp

ATGGATTTCACGGCGCTGCGGGCGGCGGTGGAGGAGGCGGAGCTAGTCGACGCCCACGCCCACAACATCGTCGCCACCGACTCCTCCTTCCCCTTCATCAGTGCCTTCTCCGAAGCCAACGCCGATGCCTTATCCTACGCACCCCATTCCCTCTCCTTCAAGAGAAGTTTGAAGGAGGTTGCTGAGCTGTACGGCTGTGACAAGTGCTTGGATGCGGTTGAGGAGCATCGGAGAGTCGCCGGATTGGAGGCCATTAGCTCCAGATGCTTTGAGGCTGCGAGAATCACTGCGTTACTTATTGATGATGGACTGAGGCTGGACAAAATGCATGACATAGATTGGCATAGGAGTTTCGTTCCTGTGGTTGGGAGAATTCTGAGGATTGAGTCTCTGGCTGAGCAGATTCTTAGTGAGGAGTTTCCAGGTGGATCTTCTTGGACATTGGATGCGTTCACTGAAGCATTTGTTGGAAAGTTGAAGTCATATCCTTATATTGATTTTCTTAACGTCTATGCCACATTTGCTGATAACATTTTTGGCTTGAAAAGTATAGCTGCATATCGCAGCGGTCTGGAAATTAGTACACATGTCACTAGGAAAGATGTTGAGGAAGGTCTTTCTGAGGTTATACAGGCTGGAAAGCCTGTTCGCATATCAAATAAAAGTTTTATCGACTATATTTTCACTCGTAGTTTGGAGGTTGCTCTACTTTTTGACTTGCCAATGCAGATACACACAGGTTTTGGCGACAAAGATTTGGACTTGAGGCTATCCAATCCCCTTCATCTCCGGGCTGTGCTTGAGGACAAGAGATTTTCTAAAAGCCGCATTGTTCTTTTACATGCATCCTACCCGTTTTCAAAGGAAGCATCATATCTAGCCTCTGTTTATCCCCAGGTCTACCTCGACTTTGGCTTGGCTATACCCAAGCTCAGTGTCCACGGGATGGTATCTTCACTAAAGGAACTTTTGGAGCTTTCTCCAATTAAGAAGGTCATGTTCAGCACTGATGGATATGCATTTCCTGAAACTTTCTACTTAGGTGCAAAAAAGGCACGTGACGTTGTCTTTTCAGTTCTATGTGATGCCTGTGCCGATGGTGATCTCTCAATTCCGGAAGCTATTGAGGCTGCTAAGGACATCTTTTCACAAAATGCAGTTCAGTTGTACAAGATTGATAGTGCTGTCAAATCTTCTGGTTCAGCCAATAGTGTATCCTCTAACTTTGTGAAGGTGAAGAGTAATGACTCGGAAAATCATGTCTCACTTGTTCGTGTTTTCTGGGCTGATGCATCAGGACAACAAAGATGTCGTGTTGTTCCTGCTAAGCGGTTCAATGACATCGTTACAAAAAATGGTATTGGTTTGACTTTTGCTTGTATGGGAATGCCTTCTTTCACTGACGGTCCAGCTGATGAGACTAACTTGACTGGAGTGGGTGAGATCAGACTCGTGCCTGATTTATCGACTAAACGGAGAATTCCATGGGTAGAACAAGAGGAAATGGTTTTGGCCAACATGCATCTTAAACCAGGTGAAGCATGGGAATACTGCCCAAGAGAGGCCTTACGGAGGGTTTCAAAAATATTGAAAGATGAATTCAATTTGGTAATGAATGCAGGATTTGAAAATGAATTTTTTCTCTTGAAGAGTATGCTAAGGGATGGGAAAGAAGAATGGGTACCATTTGACTCAACACCCTACTGTTCCCCATCATCATATGATGCTGCTTCCCCTTTGTTTCAAGAAGTTCTCGCTGCTCTCCAGTCCTTGAATATTACTGTAGAACAGTTACATGCAGAAAGTGGGAAAGGTCAATTTGAGGTGGTTCTAGGGCACACAGCTTGCCAGCATGCTGCTGACAACTTGATTTATACCCGTGAAGTTATTAGGGCCATTGCAAGGAAACACGGATTGTTGGCAACCTTTGTCCCAAAGTATGCGCTAGATGAGATTGGCTCTGGAGCCCATGTACATCTCAGTTTGTGGCAGAATGGCACAAATGTATTCATGGCATCTGGTGGATCCTCTCAGCATGGAATGTCCAAGGTTGGGGAGGAATTTATGGCAGGGGTTTTATATCATCTCCCAGCAATTTTGGCATTTACAGCTCCAATTCCAAATAGTTACGACCGGATACAACCTAATACTTGGAGTGGAGCATACAAGTGCTGGGGAAAAGAAAACAGGGAAGCTCCATTAAGAACTGCATGCCCACCTGGAGTTCAAGCTGGTCTGGTGAGCAACTTTGAGATCAAGTCATTTGATGGGTGTGCAAATCCACACTTGGGCTTGGCTGCAATACTAGCTGCTGGCATTGATGGTCTTAGGAGACATCTTTGTCTCCCTGAACCTATTGATACAAATCCTTCTAGCCTTGAAGGAGAAATACAAAGGTTACCAACTTCACTTTTGGAGTCATTAGAAGCATTAAAGGAAGATGATCTCTTCAAAGAGTTCATTGGAGAAAAGCTATTGGTTGCCATCAAGGGAGTCCGCAAGGCAGAATTTGACTACTACGAGAAGCACAAAGATGCATACAAGCAACTTATACACCGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

854

Amino Acids

94.63

Weight (kDa)

5.64

Isoelectric Point (pI)

44.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_2	PF04909	238 - 391	5e-14	Amidohydrolase
Gln-synt_N_2	PF16952	411 - 496	7.3e-07	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	517 - 846	1.8e-91	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2340
Acc65I	GGTACC	1 cut(s)	1681
AccB1I	GGYRCC	1 cut(s)	1681
AccB7I	CCANNNNNTGG	1 cut(s)	946
AccI	GTMKAC	2 cut(s)	48, 900
AccIII	TCCGGA	1 cut(s)	1114
AclWI	GGATC	3 cut(s)	436, 2019, 2032
AcoI	YGGCCR	1 cut(s)	1512
AcsI	RAATTY	6 cut(s)	372, 1479, 1598, 1634, 2060, 2507
AcuI	CTGAAG	1 cut(s)	477
AcvI	CACGTG	1 cut(s)	1058
AcyI	GRCGYC	1 cut(s)	51
AfaI	GTAC	5 cut(s)	191, 583, 1169, 1683, 1974
AfiI	CCNNNNNNNGG	7 cut(s)	365, 946, 1561, 1572, 1902, 2052, 2288
AflIII	ACRYGT	1 cut(s)	586
AjnI	CCWGG	4 cut(s)	421, 894, 1531, 2218
AjuI	GAANNNNNNNTTGG	2 cut(s)	450, 482
AloI	GAACNNNNNNTCC	2 cut(s)	630, 662
Alw26I	GTCTC	5 cut(s)	393, 1267, 1412, 2326, 2348
AlwI	GGATC	3 cut(s)	436, 2019, 2032
Aor13HI	TCCGGA	1 cut(s)	1114
AoxI	GGCC	4 cut(s)	249, 1512, 1563, 1893
ApoI	RAATTY	6 cut(s)	372, 1479, 1598, 1634, 2060, 2507
Asp700I	GAANNNNTTC	2 cut(s)	1390, 1638
Asp718I	GGTACC	1 cut(s)	1681
AspLEI	GCGC	2 cut(s)	16, 1945
AspS9I	GGNCC	2 cut(s)	1406, 1893
AsuC2I	CCSGG	1 cut(s)	791
AsuHPI	GGTGA	5 cut(s)	1112, 1246, 1451, 1547, 2251
AvaII	GGWCC	1 cut(s)	1406
BaeGI	GKGCMC	1 cut(s)	1839
BalI	TGGCCA	1 cut(s)	1514
BamHI	GGATCC	1 cut(s)	2024
BanI	GGYRCC	1 cut(s)	1681
BanII	GRGCYC	1 cut(s)	1969
BarI	GAAGNNNNNNTAC	4 cut(s)	1665, 1697, 2373, 2405
BauI	CACGAG	1 cut(s)	1451
BbrPI	CACGTG	1 cut(s)	1058
BccI	CCATC	9 cut(s)	299, 940, 1004, 1091, 1658, 1720, 2261, 2315, 2492
BceAI	ACGGC	2 cut(s)	27, 208
BciT130I	CCWGG	4 cut(s)	423, 896, 1533, 2220
BciVI	GTATCC	2 cut(s)	1225, 2130
BcnI	CCSGG	1 cut(s)	791
BcoDI	GTCTC	5 cut(s)	393, 1267, 1412, 2326, 2348
BfaI	CTAG	7 cut(s)	44, 594, 878, 1832, 1946, 2306, 2373
BfmI	CTRYAG	1 cut(s)	1782
BfoI	RGCGCY	1 cut(s)	17
BfuI	GTATCC	2 cut(s)	1225, 2130
BlpI	GCTNAGC	3 cut(s)	183, 396, 1326
Bme1390I	CCNGG	5 cut(s)	423, 791, 896, 1533, 2220
Bme18I	GGWCC	1 cut(s)	1406
BmgT120I	GGNCC	2 cut(s)	1406, 1893
BmiI	GGNNCC	3 cut(s)	1683, 1966, 2026
BmrFI	CCNGG	5 cut(s)	423, 791, 896, 1533, 2220
BplI	GAGNNNNNCTC	2 cut(s)	1943, 1975
BpmI	CTGGAG	5 cut(s)	244, 1452, 1748, 1983, 2241
Bpu10I	CCTNAGC	2 cut(s)	1134, 1658
Bpu1102I	GCTNAGC	3 cut(s)	183, 396, 1326
BpuEI	CTTGAG	2 cut(s)	784, 821
BpuMI	CCSGG	1 cut(s)	791
BsaAI	YACGTR	1 cut(s)	1058
BsaBI	GATNNNNATC	2 cut(s)	1101, 2364
BsaHI	GRCGYC	1 cut(s)	51
BsaJI	CCNNGG	4 cut(s)	894, 940, 1484, 2046
BsaWI	WCCGGW	2 cut(s)	1114, 2133
BsaXI	ACNNNNNCTCC	2 cut(s)	1956, 1986
Bsc4I	CCNNNNNNNGG	7 cut(s)	365, 946, 1561, 1572, 1902, 2052, 2288
Bse1I	ACTGG	2 cut(s)	1435, 1765
Bse3DI	GCAATG	1 cut(s)	1896
Bse8I	GATNNNNATC	2 cut(s)	1101, 2364
BseAI	TCCGGA	1 cut(s)	1114
BseBI	CCWGG	4 cut(s)	423, 896, 1533, 2220
BseDI	CCNNGG	4 cut(s)	894, 940, 1484, 2046
BseGI	GGATG	5 cut(s)	217, 451, 845, 951, 1669
BseJI	GATNNNNATC	2 cut(s)	1101, 2364
BseLI	CCNNNNNNNGG	7 cut(s)	365, 946, 1561, 1572, 1902, 2052, 2288
BseMI	GCAATG	1 cut(s)	1896
BseMII	CTCAG	8 cut(s)	174, 302, 368, 387, 612, 946, 1993, 2045
BseNI	ACTGG	2 cut(s)	1435, 1765
BseRI	GAGGAG	4 cut(s)	47, 73, 236, 428
BseSI	GKGCMC	1 cut(s)	1839
BseYI	CCCAGC	2 cut(s)	2089, 2172
Bsh1285I	CGRYCG	1 cut(s)	2134
BshFI	GGCC	4 cut(s)	251, 1514, 1565, 1895
BshNI	GGYRCC	1 cut(s)	1681
BsiEI	CGRYCG	1 cut(s)	2134
BsiSI	CCGG	4 cut(s)	240, 790, 1115, 2134
BslFI	GGGAC	1 cut(s)	1916
BslI	CCNNNNNNNGG	7 cut(s)	365, 946, 1561, 1572, 1902, 2052, 2288
BsmAI	GTCTC	5 cut(s)	393, 1267, 1412, 2326, 2348
BsmFI	GGGAC	1 cut(s)	1916
BsmI	GAATGC	2 cut(s)	1392, 1621
BsnI	GGCC	4 cut(s)	251, 1514, 1565, 1895
Bsp1286I	GDGCHC	2 cut(s)	1839, 1969
Bsp13I	TCCGGA	1 cut(s)	1114
Bsp1407I	TGTACA	2 cut(s)	1167, 1972
Bsp143I	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
Bsp1720I	GCTNAGC	3 cut(s)	183, 396, 1326
Bsp19I	CCATGG	1 cut(s)	1484
BspANI	GGCC	4 cut(s)	251, 1514, 1565, 1895
BspCNI	CTCAG	8 cut(s)	175, 303, 369, 388, 613, 945, 1992, 2044
BspEI	TCCGGA	1 cut(s)	1114
BspLI	GGNNCC	3 cut(s)	1683, 1966, 2026
BspPI	GGATC	3 cut(s)	436, 2019, 2032
BspT107I	GGYRCC	1 cut(s)	1681
BsrDI	GCAATG	1 cut(s)	1896
BsrGI	TGTACA	2 cut(s)	1167, 1972
BsrI	ACTGG	2 cut(s)	1435, 1765
BssECI	CCNNGG	4 cut(s)	894, 940, 1484, 2046
BssMI	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
BssNI	GRCGYC	1 cut(s)	51
BssSI	CACGAG	1 cut(s)	1451
BssT1I	CCWWGG	2 cut(s)	1484, 2046
Bst2BI	CACGAG	1 cut(s)	1451
Bst2UI	CCWGG	4 cut(s)	423, 896, 1533, 2220
Bst4CI	ACNGT	4 cut(s)	1406, 1706, 1783, 1791
Bst6I	CTCTTC	3 cut(s)	1232, 1643, 2453
BstACI	GRCGYC	1 cut(s)	51
BstAUI	TGTACA	2 cut(s)	1167, 1972
BstBAI	YACGTR	1 cut(s)	1058
BstC8I	GCNNGC	6 cut(s)	21, 1846, 1850, 1854, 2212, 2314
BstDSI	CCRYGG	2 cut(s)	940, 1484
BstEII	GGTNACC	1 cut(s)	2397
BstENI	CCTNNNNNAGG	1 cut(s)	1570
BstF5I	GGATG	5 cut(s)	217, 451, 845, 951, 1669
BstH2I	RGCGCY	1 cut(s)	17
BstHHI	GCGC	2 cut(s)	16, 1945
BstKTI	GATC	6 cut(s)	431, 1105, 1446, 2027, 2256, 2446
BstMAI	GTCTC	5 cut(s)	393, 1267, 1412, 2326, 2348
BstMBI	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
BstMCI	CGRYCG	1 cut(s)	2134
BstMWI	GCNNNNNNNGC	8 cut(s)	20, 264, 521, 1052, 1127, 2099, 2108, 2306
BstNI	CCWGG	4 cut(s)	423, 896, 1533, 2220
BstNSI	RCATGY	6 cut(s)	590, 845, 1522, 1799, 1856, 2214
BstPI	GGTNACC	1 cut(s)	2397
BstSCI	CCNGG	5 cut(s)	421, 789, 894, 1531, 2218
BstSFI	CTRYAG	1 cut(s)	1782
BstSLI	GKGCMC	1 cut(s)	1839
BstX2I	RGATCY	2 cut(s)	428, 2024
BstYI	RGATCY	2 cut(s)	428, 2024
BsuI	GTATCC	2 cut(s)	1225, 2130
BsuRI	GGCC	4 cut(s)	251, 1514, 1565, 1895
BtgI	CCRYGG	2 cut(s)	940, 1484
BtsCI	GGATG	5 cut(s)	217, 451, 845, 951, 1669
BtsI	GCAGTG	1 cut(s)	285
BtsIMutI	CAGTG	6 cut(s)	106, 285, 453, 940, 1005, 1398
Cac8I	GCNNGC	6 cut(s)	21, 1846, 1850, 1854, 2212, 2314
CfoI	GCGC	2 cut(s)	16, 1945
Cfr13I	GGNCC	2 cut(s)	1406, 1893
CseI	GACGC	1 cut(s)	59
CsiI	ACCWGGT	1 cut(s)	1531
Csp6I	GTAC	5 cut(s)	190, 582, 1168, 1682, 1973
CviQI	GTAC	5 cut(s)	190, 582, 1168, 1682, 1973
DpnI	GATC	6 cut(s)	430, 1104, 1445, 2026, 2255, 2445
DpnII	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
DrdI	GACNNNNNNGTC	1 cut(s)	2340
DseDI	GACNNNNNNGTC	1 cut(s)	2340
EaeI	YGGCCR	1 cut(s)	1512
Eam1104I	CTCTTC	3 cut(s)	1232, 1643, 2453
EarI	CTCTTC	3 cut(s)	1232, 1643, 2453
EciI	GGCGGA	1 cut(s)	53
Eco130I	CCWWGG	2 cut(s)	1484, 2046
Eco147I	AGGCCT	1 cut(s)	1565
Eco24I	GRGCYC	1 cut(s)	1969
Eco47I	GGWCC	1 cut(s)	1406
Eco57I	CTGAAG	1 cut(s)	477
Eco72I	CACGTG	1 cut(s)	1058
Eco91I	GGTNACC	1 cut(s)	2397
EcoNI	CCTNNNNNAGG	1 cut(s)	1570
EcoO65I	GGTNACC	1 cut(s)	2397
EcoRI	GAATTC	3 cut(s)	372, 1479, 1598
EcoRII	CCWGG	4 cut(s)	421, 894, 1531, 2218
EcoT14I	CCWWGG	2 cut(s)	1484, 2046
EcoT22I	ATGCAT	6 cut(s)	330, 847, 1021, 1294, 1524, 2539
EcoT38I	GRGCYC	1 cut(s)	1969
ErhI	CCWWGG	2 cut(s)	1484, 2046
FalI	AAGNNNNNCTT	2 cut(s)	1127, 1159
FaqI	GGGAC	1 cut(s)	1916
FauI	CCCGC	1 cut(s)	12
FauNDI	CATATG	1 cut(s)	1720
FblI	GTMKAC	2 cut(s)	48, 900
FokI	GGATG	5 cut(s)	224, 458, 832, 958, 1676
FriOI	GRGCYC	1 cut(s)	1969
FspBI	CTAG	7 cut(s)	44, 594, 878, 1832, 1946, 2306, 2373
GlaI	GCGC	2 cut(s)	15, 1944
GsaI	CCCAGC	2 cut(s)	2093, 2176
GsuI	CTGGAG	5 cut(s)	244, 1452, 1748, 1983, 2241
HaeII	RGCGCY	1 cut(s)	17
HaeIII	GGCC	4 cut(s)	251, 1514, 1565, 1895
HapII	CCGG	4 cut(s)	240, 790, 1115, 2134
HgaI	GACGC	1 cut(s)	59
HhaI	GCGC	2 cut(s)	16, 1945
Hin1I	GRCGYC	1 cut(s)	51
Hin6I	GCGC	2 cut(s)	14, 1943
HinP1I	GCGC	2 cut(s)	14, 1943
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HpaII	CCGG	4 cut(s)	240, 790, 1115, 2134
HphI	GGTGA	5 cut(s)	1112, 1246, 1451, 1547, 2251
Hpy166II	GTNNAC	5 cut(s)	49, 453, 584, 901, 940
Hpy188I	TCNGA	6 cut(s)	112, 231, 378, 622, 1252, 1448
Hpy188III	TCNNGA	9 cut(s)	160, 261, 572, 1025, 1115, 1296, 1645, 1745, 1962
Hpy8I	GTNNAC	5 cut(s)	49, 453, 584, 901, 940
Hpy99I	CGWCG	2 cut(s)	53, 74
HpyCH4III	ACNGT	4 cut(s)	1406, 1706, 1783, 1791
HpyCH4IV	ACGT	3 cut(s)	507, 1057, 1062
HpyF10VI	GCNNNNNNNGC	8 cut(s)	20, 264, 521, 1052, 1127, 2099, 2108, 2306
HpySE526I	ACGT	3 cut(s)	507, 1057, 1062
Hsp92I	GRCGYC	1 cut(s)	51
HspAI	GCGC	2 cut(s)	14, 1943
Kpn2I	TCCGGA	1 cut(s)	1114
KpnI	GGTACC	1 cut(s)	1685
Kzo9I	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
LmnI	GCTCC	8 cut(s)	40, 223, 263, 973, 1964, 2116, 2159, 2200
MabI	ACCWGGT	1 cut(s)	1531
MaeI	CTAG	7 cut(s)	44, 594, 878, 1832, 1946, 2306, 2373
MaeII	ACGT	3 cut(s)	507, 1057, 1062
MaeIII	GTNAC	8 cut(s)	197, 291, 589, 1058, 1345, 1791, 2126, 2397
MalI	GATC	6 cut(s)	430, 1104, 1445, 2026, 2255, 2445
MboI	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
MboII	GAAGA	8 cut(s)	423, 945, 1185, 1249, 1660, 1685, 2440, 2450
MflI	RGATCY	2 cut(s)	428, 2024
MhlI	GDGCHC	2 cut(s)	1839, 1969
MlsI	TGGCCA	1 cut(s)	1514
MluNI	TGGCCA	1 cut(s)	1514
MlyI	GAGTC	8 cut(s)	74, 243, 395, 1241, 1443, 1685, 2426, 2502
MmeI	TCCRAC	1 cut(s)	448
Mox20I	TGGCCA	1 cut(s)	1514
Mph1103I	ATGCAT	6 cut(s)	330, 847, 1021, 1294, 1524, 2539
MroI	TCCGGA	1 cut(s)	1114
MroXI	GAANNNNTTC	2 cut(s)	1390, 1638
MscI	TGGCCA	1 cut(s)	1514
MseI	TTAA	5 cut(s)	504, 987, 1527, 2201, 2432
MslI	CAYNNNNRTG	2 cut(s)	2167, 2532
Msp20I	TGGCCA	1 cut(s)	1514
MspA1I	CMGCKG	2 cut(s)	567, 1412
MspI	CCGG	4 cut(s)	240, 790, 1115, 2134
MspR9I	CCNGG	5 cut(s)	423, 791, 896, 1533, 2220
Mva1269I	GAATGC	2 cut(s)	1392, 1621
MvaI	CCWGG	4 cut(s)	423, 896, 1533, 2220
MwoI	GCNNNNNNNGC	8 cut(s)	20, 264, 521, 1052, 1127, 2099, 2108, 2306
NciI	CCSGG	1 cut(s)	791
NcoI	CCATGG	1 cut(s)	1484
NdeI	CATATG	1 cut(s)	1720
NdeII	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
NlaIV	GGNNCC	3 cut(s)	1683, 1966, 2026
NmuCI	GTSAC	3 cut(s)	197, 589, 1058
NsiI	ATGCAT	6 cut(s)	330, 847, 1021, 1294, 1524, 2539
NspI	RCATGY	6 cut(s)	590, 845, 1522, 1799, 1856, 2214
PaeI	GCATGC	2 cut(s)	1856, 2214
PceI	AGGCCT	1 cut(s)	1565
PciI	ACATGT	1 cut(s)	586
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	2 cut(s)	1392, 1621
PdmI	GAANNNNTTC	2 cut(s)	1390, 1638
PfeI	GAWTC	2 cut(s)	282, 403
PflFI	GACNNNGTC	1 cut(s)	1064
PflMI	CCANNNNNTGG	1 cut(s)	946
PleI	GAGTC	8 cut(s)	74, 242, 394, 1241, 1443, 1685, 2425, 2501
PmaCI	CACGTG	1 cut(s)	1058
PmlI	CACGTG	1 cut(s)	1058
PpsI	GAGTC	8 cut(s)	74, 242, 394, 1241, 1443, 1685, 2425, 2501
Ppu21I	YACGTR	1 cut(s)	1058
PscI	ACATGT	1 cut(s)	586
Psp6I	CCWGG	4 cut(s)	421, 894, 1531, 2218
PspCI	CACGTG	1 cut(s)	1058
PspEI	GGTNACC	1 cut(s)	2397
PspFI	CCCAGC	2 cut(s)	2089, 2172
PspGI	CCWGG	4 cut(s)	421, 894, 1531, 2218
PspN4I	GGNNCC	3 cut(s)	1683, 1966, 2026
PspPI	GGNCC	2 cut(s)	1406, 1893
PsuI	RGATCY	2 cut(s)	428, 2024
PsyI	GACNNNGTC	1 cut(s)	1064
PvuII	CAGCTG	1 cut(s)	1412
RsaI	GTAC	5 cut(s)	191, 583, 1169, 1683, 1974
RsaNI	GTAC	5 cut(s)	190, 582, 1168, 1682, 1973
RseI	CAYNNNNRTG	2 cut(s)	2167, 2532
SalI	GTCGAC	1 cut(s)	47
SaqAI	TTAA	5 cut(s)	504, 987, 1527, 2201, 2432
Sau3AI	GATC	6 cut(s)	428, 1102, 1443, 2024, 2253, 2443
Sau96I	GGNCC	2 cut(s)	1406, 1893
SchI	GAGTC	8 cut(s)	74, 243, 395, 1241, 1443, 1685, 2426, 2502
ScrFI	CCNGG	5 cut(s)	423, 791, 896, 1533, 2220
SduI	GDGCHC	2 cut(s)	1839, 1969
SexAI	ACCWGGT	1 cut(s)	1531
SfcI	CTRYAG	1 cut(s)	1782
SinI	GGWCC	1 cut(s)	1406
SmiMI	CAYNNNNRTG	2 cut(s)	2167, 2532
SmlI	CTYRAG	2 cut(s)	763, 800
SmoI	CTYRAG	2 cut(s)	763, 800
SphI	GCATGC	2 cut(s)	1856, 2214
SseBI	AGGCCT	1 cut(s)	1565
SspI	AATATT	2 cut(s)	1587, 1777
SspMI	CTAG	7 cut(s)	44, 594, 878, 1832, 1946, 2306, 2373
StuI	AGGCCT	1 cut(s)	1565
StyD4I	CCNGG	5 cut(s)	421, 789, 894, 1531, 2218
StyI	CCWWGG	2 cut(s)	1484, 2046
TaaI	ACNGT	4 cut(s)	1406, 1706, 1783, 1791
TaiI	ACGT	3 cut(s)	510, 1060, 1065
TaqI	TCGA	4 cut(s)	48, 672, 906, 1466
TatI	WGTACW	3 cut(s)	581, 1167, 1972
TauI	GCSGC	2 cut(s)	26, 828
TfiI	GAWTC	2 cut(s)	282, 403
Tru1I	TTAA	5 cut(s)	504, 987, 1527, 2201, 2432
Tru9I	TTAA	5 cut(s)	504, 987, 1527, 2201, 2432
TscAI	CASTG	6 cut(s)	106, 292, 460, 940, 1012, 1405
TseFI	GTSAC	3 cut(s)	197, 589, 1058
Tsp45I	GTSAC	3 cut(s)	197, 589, 1058
TspDTI	ATGAA	7 cut(s)	85, 773, 1611, 1628, 1647, 1999, 2449
TspGWI	ACGGA	3 cut(s)	1489, 1585, 1926
TspRI	CASTG	6 cut(s)	106, 292, 460, 940, 1012, 1405
Tth111I	GACNNNGTC	1 cut(s)	1064
Van91I	CCANNNNNTGG	1 cut(s)	946
VpaK11BI	GGWCC	1 cut(s)	1406
XagI	CCTNNNNNAGG	1 cut(s)	1570
XapI	RAATTY	6 cut(s)	372, 1479, 1598, 1634, 2060, 2507
XceI	RCATGY	6 cut(s)	590, 845, 1522, 1799, 1856, 2214
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1539
XmiI	GTMKAC	2 cut(s)	48, 900
XmnI	GAANNNNTTC	2 cut(s)	1390, 1638
XspI	CTAG	7 cut(s)	44, 594, 878, 1832, 1946, 2306, 2373
Zsp2I	ATGCAT	6 cut(s)	330, 847, 1021, 1294, 1524, 2539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.