Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Reverse (-)

15895333 .. 15902545

Gene Structure 7213 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G015430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGGATTTCACGGCGCTCCGGGCGGCGGTGGAGGAGGCGGAGCTAGTCGACGCCCACGCCCACAACATCGTCGCCGTCGACTCCTCCTTCCCCTTCATCAGTGCCTTCTCCGAAGCCAACGCCGACGCCTTATCCTACGCACCCCATTCCCTCTCCTTCAAGAGAAGTTTGAAGGAGGTTGCGGAGCTTTACGGCTGTGAGAAGACCTTGGATGCGGTTGAGGAGCATCGGAGAGTCGCCGGATTAGAGGCGATTAGCTCCCGGTGCTTTGAGGCTGCGGGAATCTCCGCATTGCTTATTGATGATGGACTGAGGCTGGACAAAATGCATGACGTAGATTGGCACAAGAGTTTCGCGCCTGTGGTTGGGAGGATTCTCAGGATTGAGTTCCTGGCTGAGCAGATTCTTAATGAGGAGTTGCCGGGTGGATCTTCTTGGACATTGGATGTGTTCACTGAAGCATTTATTTTTGGCTTGAAAAGTATAGCTGCATATCGCAGCGGTCTGGAAATTAGGACACATGTCACTAGGAAAGATGTTGAGGAAGGTCTTTCTGAGGTCATACAGGCTGGAAAGCCTGTTCGCATATCAAATAAAAGTTTTATCGACTATATTTTCACTCGTAGTTTGGAGGTTGCTCTACTTTTTGACTTGCCAATGCAGATACATACAGGTTTTGGCGACAAAGATTTGGACTTGAGGCTATCCAATCCCCTTCATCTCCGGGCTGTGCTTGAGGACAAGAGATTTTCTAAAAGCCGCATTGTTCTTTTACATGCATCCTACCCGTTTTCAAAGGAAGCATCATATCTAGCCTCTGTTTATCCCCAGGTCTACCTCGACTTTGGCTTGGCTATACCCAAGCTCAGTGTCCACGGGATGGTATCTTCACTAAAAGAACTTTTGGAGCTTGCTCCAATTAAGAAGGTCATGTTCAGCACTGATGGATATGCATTTCCCGAAACTTTCTACTTAGGTGCAAAAAAAGCACGTGAAGTTGTCCTTTCAGTTCTATCTGATGCCTGTGCTGATGGTGATCTCTCAATTCCGGAAGCTATTGAGGCTGCTGAAGACATCTTTTCACAAAATGCAGTTCAGTTTTACAAGATTGATCGTGCTGTCAAATCTTCTGGTTCAGCCAATAGTGTATGCTCTAACTTGGCGAAGGTGAAGAGTAATGACTCGGAAAATCATGTCTCACTTGTTCGTGTTTTCTGGGCTGATGCATCAGGACAACAAAGATGTCGTGTTGTTCCTGCTAAGCGGTTCAATGACATCGTTACAAAAAATGGCATTGGTTTGACTTTTGCTTGTATGGGAATGACTTCTTTCACTGATGGTCCAGCTGATGAGACTAATTTGACCGGAGTGGGTGAGATCAGACTCATGCCTGATTTATCGACTAAGTGGAGAATTCCATGGGTAGAACAAGAGGAAATGGTTTTGGCCAACATGCATCTTAAACCAGGTGAAGCATGGGAATACTGCCCAAGAGAGGCCTTACGGAGGGTTTCAAAAATATTGAAAGATGAATTCAATTTGGTAATGAATGCAGGATTTGAAAATGAATTTTTTCTCTTGAAGAGTATACTAAGGGACGGGAAAGAAGAATGGGTACCATTTGACTCAACACCCTACTGTTCCCCATCATCATATGATGCTGCTTCCCCTTTGTTTCACGAAGTTCTTGCTGCTCTCCAGTCCTTGAATATTACTGTAGAACAGTTACATGCAGAAAGTGGGAAAGGTCAATTTGAGATGGCTCTAGGGCACACGGCTTGCCAGCATGCTGCTGACAACTTGATTTATACCCGTGAAGTTATTAGGGCCATTGCAAGGAAACACGAATTGTTGGCAACCTTTGTGCCAAAGTATGCGCTAGATGATATTGGCTCTGGAGCCCATGTACATCTCAGTTTGTGGCAGAATGGGAAAAATGTATTCATGGCATCTGGTGGATCCTCTCAGCATGGAATGTCCAAGGTTGGGGAGGAATTTATGGCAGGGGTTTTATATCATCTCCCTGCAATTTTGGCATTTACAGCTCCAATTCCAAATAGTTACGACCGGATACAACCTAATACTTGGAGTGGAGCATACAAGTGCTGGGGAAAAGAAAACAGGGAAGCTCCATTAAGAACTGCATGCCCACCTGGAATTCAAGCTGGTCTGGTGAGCAACTTTGAGATCAAGTCATTTGATGGGTGTGCAAATCCACACTTGGGCTTGGCTGCAATACTAGCTGCTGGCATTGATGGCCTTAGGGGGCATCTTTGTCTCCCTGAACCTATTGATACAAATCCTTCTAGCCTTGATGGAGAACTACAAAGGTTACCAACTTCACTTTTGGAGTCATTAGAAGCTTTAAAGGAAGATGATCTCTTCAAAGAGTTCATTGGAGAAAAGCTATTGGTTGCCATAAAGGGAGTCCGCAAGGCAGAATTTGACTACTACGAGAAGCACAAAGATGCATACAAGCAACTTATACACCGGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

832

Amino Acids

92.14

Weight (kDa)

5.55

Isoelectric Point (pI)

40.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_2	PF04909	216 - 369	2.4e-14	Amidohydrolase
Gln-synt_N_2	PF16952	392 - 474	3.7e-07	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	495 - 824	4.4e-90	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1615
AccB1I	GGYRCC	1 cut(s)	1615
AccB7I	CCANNNNNTGG	1 cut(s)	880
AccI	GTMKAC	4 cut(s)	48, 78, 834, 1588
AccII	CGCG	1 cut(s)	356
AccIII	TCCGGA	1 cut(s)	1048
AclWI	GGATC	3 cut(s)	436, 1953, 1966
AcoI	YGGCCR	1 cut(s)	1446
AcsI	RAATTY	6 cut(s)	1413, 1532, 1568, 1994, 2157, 2441
AcuI	CTGAAG	2 cut(s)	477, 1089
AcvI	CACGTG	1 cut(s)	992
AcyI	GRCGYC	2 cut(s)	51, 126
AfaI	GTAC	2 cut(s)	1617, 1908
AfiI	CCNNNNNNNGG	8 cut(s)	25, 365, 880, 1495, 1506, 1836, 1986, 2222
AflIII	ACRYGT	1 cut(s)	520
AgeI	ACCGGT	1 cut(s)	2490
AjnI	CCWGG	4 cut(s)	390, 828, 1465, 2152
AloI	GAACNNNNNNTCC	2 cut(s)	564, 596
Alw26I	GTCTC	3 cut(s)	1201, 1346, 2282
AlwI	GGATC	3 cut(s)	436, 1953, 1966
Aor13HI	TCCGGA	1 cut(s)	1048
AoxI	GGCC	4 cut(s)	1446, 1497, 1827, 2257
ApeKI	GCWGC	9 cut(s)	275, 488, 498, 1064, 1661, 1691, 1790, 2231, 2243
ApoI	RAATTY	6 cut(s)	1413, 1532, 1568, 1994, 2157, 2441
AsiGI	ACCGGT	1 cut(s)	2490
Asp700I	GAANNNNTTC	2 cut(s)	1324, 1572
Asp718I	GGTACC	1 cut(s)	1615
AspLEI	GCGC	3 cut(s)	16, 358, 1879
AspS9I	GGNCC	2 cut(s)	1340, 1827
AsuC2I	CCSGG	4 cut(s)	20, 262, 423, 725
AsuHPI	GGTGA	5 cut(s)	1046, 1180, 1385, 1481, 2185
AvaII	GGWCC	1 cut(s)	1340
AxyI	CCTNAGG	1 cut(s)	2261
BaeGI	GKGCMC	1 cut(s)	1773
BalI	TGGCCA	1 cut(s)	1448
BamHI	GGATCC	1 cut(s)	1958
BanI	GGYRCC	1 cut(s)	1615
BanII	GRGCYC	1 cut(s)	1903
BarI	GAAGNNNNNNTAC	2 cut(s)	1599, 1631
BbrPI	CACGTG	1 cut(s)	992
BbsI	GAAGAC	2 cut(s)	209, 1077
BbvI	GCAGC	9 cut(s)	262, 475, 510, 1051, 1648, 1678, 1777, 2218, 2230
BceAI	ACGGC	4 cut(s)	27, 59, 208, 1791
BcgI	CGANNNNNNTGC	2 cut(s)	1670, 1704
BciT130I	CCWGG	4 cut(s)	392, 830, 1467, 2154
BciVI	GTATCC	1 cut(s)	2064
BcnI	CCSGG	4 cut(s)	20, 262, 423, 725
BcoDI	GTCTC	3 cut(s)	1201, 1346, 2282
BfaI	CTAG	7 cut(s)	44, 528, 812, 1766, 1880, 2240, 2307
BfmI	CTRYAG	1 cut(s)	1716
BfoI	RGCGCY	1 cut(s)	17
BfuI	GTATCC	1 cut(s)	2064
BlpI	GCTNAGC	2 cut(s)	396, 1260
Bme1390I	CCNGG	8 cut(s)	20, 262, 392, 423, 725, 830, 1467, 2154
Bme18I	GGWCC	1 cut(s)	1340
BmgT120I	GGNCC	2 cut(s)	1340, 1827
BmiI	GGNNCC	3 cut(s)	1617, 1900, 1960
BmrFI	CCNGG	8 cut(s)	20, 262, 392, 423, 725, 830, 1467, 2154
BoxI	GACNNNNGTC	1 cut(s)	521
BpiI	GAAGAC	2 cut(s)	209, 1077
BpmI	CTGGAG	2 cut(s)	1682, 1917
Bpu1102I	GCTNAGC	2 cut(s)	396, 1260
BpuEI	CTTGAG	2 cut(s)	718, 755
BpuMI	CCSGG	4 cut(s)	20, 262, 423, 725
BsaAI	YACGTR	1 cut(s)	992
BsaBI	GATNNNNATC	2 cut(s)	1035, 2298
BsaHI	GRCGYC	2 cut(s)	51, 126
BsaJI	CCNNGG	5 cut(s)	207, 828, 874, 1418, 1980
BsaWI	WCCGGW	4 cut(s)	1048, 1364, 2067, 2490
BsaXI	ACNNNNNCTCC	2 cut(s)	1890, 1920
Bsc4I	CCNNNNNNNGG	8 cut(s)	25, 365, 880, 1495, 1506, 1836, 1986, 2222
Bse118I	RCCGGY	1 cut(s)	2490
Bse1I	ACTGG	1 cut(s)	1699
Bse21I	CCTNAGG	1 cut(s)	2261
Bse3DI	GCAATG	2 cut(s)	290, 1830
Bse8I	GATNNNNATC	2 cut(s)	1035, 2298
BseAI	TCCGGA	1 cut(s)	1048
BseBI	CCWGG	4 cut(s)	392, 830, 1467, 2154
BseDI	CCNNGG	5 cut(s)	207, 828, 874, 1418, 1980
BseGI	GGATG	4 cut(s)	217, 451, 779, 885
BseJI	GATNNNNATC	2 cut(s)	1035, 2298
BseLI	CCNNNNNNNGG	8 cut(s)	25, 365, 880, 1495, 1506, 1836, 1986, 2222
BseMI	GCAATG	2 cut(s)	290, 1830
BseMII	CTCAG	7 cut(s)	302, 387, 391, 546, 880, 1927, 1979
BseNI	ACTGG	1 cut(s)	1699
BseRI	GAGGAG	4 cut(s)	47, 73, 236, 428
BseSI	GKGCMC	1 cut(s)	1773
BseXI	GCAGC	9 cut(s)	262, 475, 510, 1051, 1648, 1678, 1777, 2218, 2230
BseYI	CCCAGC	1 cut(s)	2106
Bsh1236I	CGCG	1 cut(s)	356
Bsh1285I	CGRYCG	1 cut(s)	2068
BshFI	GGCC	4 cut(s)	1448, 1499, 1829, 2259
BshNI	GGYRCC	1 cut(s)	1615
BshTI	ACCGGT	1 cut(s)	2490
BsiEI	CGRYCG	1 cut(s)	2068
BsiSI	CCGG	9 cut(s)	19, 240, 262, 422, 724, 1049, 1365, 2068, 2491
BslFI	GGGAC	1 cut(s)	1610
BslI	CCNNNNNNNGG	8 cut(s)	25, 365, 880, 1495, 1506, 1836, 1986, 2222
BsmAI	GTCTC	3 cut(s)	1201, 1346, 2282
BsmFI	GGGAC	1 cut(s)	1610
BsmI	GAATGC	1 cut(s)	1555
BsnI	GGCC	4 cut(s)	1448, 1499, 1829, 2259
Bsp1286I	GDGCHC	2 cut(s)	1773, 1903
Bsp13I	TCCGGA	1 cut(s)	1048
Bsp1407I	TGTACA	1 cut(s)	1906
Bsp143I	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
Bsp1720I	GCTNAGC	2 cut(s)	396, 1260
Bsp19I	CCATGG	1 cut(s)	1418
BspANI	GGCC	4 cut(s)	1448, 1499, 1829, 2259
BspCNI	CTCAG	7 cut(s)	303, 388, 390, 547, 879, 1926, 1978
BspEI	TCCGGA	1 cut(s)	1048
BspFNI	CGCG	1 cut(s)	356
BspLI	GGNNCC	3 cut(s)	1617, 1900, 1960
BspPI	GGATC	3 cut(s)	436, 1953, 1966
BspT107I	GGYRCC	1 cut(s)	1615
BsrDI	GCAATG	2 cut(s)	290, 1830
BsrFI	RCCGGY	1 cut(s)	2490
BsrGI	TGTACA	1 cut(s)	1906
BsrI	ACTGG	1 cut(s)	1699
BssAI	RCCGGY	1 cut(s)	2490
BssECI	CCNNGG	5 cut(s)	207, 828, 874, 1418, 1980
BssMI	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
BssNAI	GTATAC	1 cut(s)	1589
BssNI	GRCGYC	2 cut(s)	51, 126
BssT1I	CCWWGG	3 cut(s)	207, 1418, 1980
Bst1107I	GTATAC	1 cut(s)	1589
Bst2UI	CCWGG	4 cut(s)	392, 830, 1467, 2154
Bst4CI	ACNGT	3 cut(s)	1640, 1717, 1725
Bst6I	CTCTTC	3 cut(s)	1166, 1577, 2387
BstACI	GRCGYC	2 cut(s)	51, 126
BstAUI	TGTACA	1 cut(s)	1906
BstBAI	YACGTR	1 cut(s)	992
BstC8I	GCNNGC	6 cut(s)	912, 1780, 1784, 1788, 2146, 2248
BstDSI	CCRYGG	2 cut(s)	874, 1418
BstEII	GGTNACC	1 cut(s)	2331
BstENI	CCTNNNNNAGG	1 cut(s)	1504
BstF5I	GGATG	4 cut(s)	217, 451, 779, 885
BstFNI	CGCG	1 cut(s)	356
BstH2I	RGCGCY	1 cut(s)	17
BstHHI	GCGC	3 cut(s)	16, 358, 1879
BstKTI	GATC	7 cut(s)	431, 1039, 1114, 1380, 1961, 2190, 2380
BstMAI	GTCTC	3 cut(s)	1201, 1346, 2282
BstMBI	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
BstMCI	CGRYCG	1 cut(s)	2068
BstMWI	GCNNNNNNNGC	8 cut(s)	20, 264, 986, 1061, 2033, 2042, 2240, 2256
BstNI	CCWGG	4 cut(s)	392, 830, 1467, 2154
BstNSI	RCATGY	6 cut(s)	524, 779, 1456, 1733, 1790, 2148
BstPAI	GACNNNNGTC	1 cut(s)	521
BstPI	GGTNACC	1 cut(s)	2331
BstSCI	CCNGG	8 cut(s)	18, 260, 390, 421, 723, 828, 1465, 2152
BstSFI	CTRYAG	1 cut(s)	1716
BstSLI	GKGCMC	1 cut(s)	1773
BstUI	CGCG	1 cut(s)	356
BstV1I	GCAGC	9 cut(s)	262, 475, 510, 1051, 1648, 1678, 1777, 2218, 2230
BstV2I	GAAGAC	2 cut(s)	209, 1077
BstX2I	RGATCY	2 cut(s)	428, 1958
BstYI	RGATCY	2 cut(s)	428, 1958
BstZ17I	GTATAC	1 cut(s)	1589
Bsu36I	CCTNAGG	1 cut(s)	2261
BsuI	GTATCC	1 cut(s)	2064
BsuRI	GGCC	4 cut(s)	1448, 1499, 1829, 2259
BtgI	CCRYGG	2 cut(s)	874, 1418
BtsCI	GGATG	4 cut(s)	217, 451, 779, 885
BtsIMutI	CAGTG	5 cut(s)	106, 453, 874, 939, 1332
Cac8I	GCNNGC	6 cut(s)	912, 1780, 1784, 1788, 2146, 2248
CfoI	GCGC	3 cut(s)	16, 358, 1879
Cfr10I	RCCGGY	1 cut(s)	2490
Cfr13I	GGNCC	2 cut(s)	1340, 1827
CseI	GACGC	2 cut(s)	59, 134
CsiI	ACCWGGT	1 cut(s)	1465
Csp6I	GTAC	2 cut(s)	1616, 1907
CspAI	ACCGGT	1 cut(s)	2490
CviQI	GTAC	2 cut(s)	1616, 1907
DpnI	GATC	7 cut(s)	430, 1038, 1113, 1379, 1960, 2189, 2379
DpnII	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
DraI	TTTAAA	1 cut(s)	2367
EaeI	YGGCCR	1 cut(s)	1446
Eam1104I	CTCTTC	3 cut(s)	1166, 1577, 2387
EarI	CTCTTC	3 cut(s)	1166, 1577, 2387
EciI	GGCGGA	1 cut(s)	53
Eco130I	CCWWGG	3 cut(s)	207, 1418, 1980
Eco147I	AGGCCT	1 cut(s)	1499
Eco24I	GRGCYC	1 cut(s)	1903
Eco47I	GGWCC	1 cut(s)	1340
Eco57I	CTGAAG	2 cut(s)	477, 1089
Eco72I	CACGTG	1 cut(s)	992
Eco81I	CCTNAGG	1 cut(s)	2261
Eco91I	GGTNACC	1 cut(s)	2331
EcoNI	CCTNNNNNAGG	1 cut(s)	1504
EcoO65I	GGTNACC	1 cut(s)	2331
EcoRI	GAATTC	3 cut(s)	1413, 1532, 2157
EcoRII	CCWGG	4 cut(s)	390, 828, 1465, 2152
EcoT14I	CCWWGG	3 cut(s)	207, 1418, 1980
EcoT22I	ATGCAT	6 cut(s)	330, 781, 955, 1228, 1458, 2473
EcoT38I	GRGCYC	1 cut(s)	1903
ErhI	CCWWGG	3 cut(s)	207, 1418, 1980
FalI	AAGNNNNNCTT	2 cut(s)	987, 1019
FaqI	GGGAC	1 cut(s)	1610
FauI	CCCGC	1 cut(s)	271
FauNDI	CATATG	1 cut(s)	1654
FblI	GTMKAC	4 cut(s)	48, 78, 834, 1588
FokI	GGATG	4 cut(s)	224, 458, 766, 892
FriOI	GRGCYC	1 cut(s)	1903
FspBI	CTAG	7 cut(s)	44, 528, 812, 1766, 1880, 2240, 2307
GlaI	GCGC	3 cut(s)	15, 357, 1878
GsaI	CCCAGC	1 cut(s)	2110
GsuI	CTGGAG	2 cut(s)	1682, 1917
HaeII	RGCGCY	1 cut(s)	17
HaeIII	GGCC	4 cut(s)	1448, 1499, 1829, 2259
HapII	CCGG	9 cut(s)	19, 240, 262, 422, 724, 1049, 1365, 2068, 2491
HgaI	GACGC	2 cut(s)	59, 134
HhaI	GCGC	3 cut(s)	16, 358, 1879
Hin1I	GRCGYC	2 cut(s)	51, 126
Hin6I	GCGC	3 cut(s)	14, 356, 1877
HinP1I	GCGC	3 cut(s)	14, 356, 1877
HincII	GTYRAC	2 cut(s)	49, 79
HindII	GTYRAC	2 cut(s)	49, 79
HindIII	AAGCTT	1 cut(s)	2361
HpaII	CCGG	9 cut(s)	19, 240, 262, 422, 724, 1049, 1365, 2068, 2491
HphI	GGTGA	5 cut(s)	1046, 1180, 1385, 1481, 2185
Hpy166II	GTNNAC	6 cut(s)	49, 79, 453, 835, 874, 1589
Hpy188I	TCNGA	6 cut(s)	112, 231, 556, 1018, 1186, 1382
Hpy188III	TCNNGA	9 cut(s)	160, 379, 506, 959, 1049, 1230, 1579, 1679, 1896
Hpy8I	GTNNAC	6 cut(s)	49, 79, 453, 835, 874, 1589
Hpy99I	CGWCG	4 cut(s)	53, 74, 80, 128
HpyCH4III	ACNGT	3 cut(s)	1640, 1717, 1725
HpyCH4IV	ACGT	2 cut(s)	333, 991
HpyF10VI	GCNNNNNNNGC	8 cut(s)	20, 264, 986, 1061, 2033, 2042, 2240, 2256
HpySE526I	ACGT	2 cut(s)	333, 991
Hsp92I	GRCGYC	2 cut(s)	51, 126
HspAI	GCGC	3 cut(s)	14, 356, 1877
Kpn2I	TCCGGA	1 cut(s)	1048
KpnI	GGTACC	1 cut(s)	1619
Kzo9I	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
Lsp1109I	GCAGC	9 cut(s)	262, 475, 510, 1051, 1648, 1678, 1777, 2218, 2230
MabI	ACCWGGT	1 cut(s)	1465
MaeI	CTAG	7 cut(s)	44, 528, 812, 1766, 1880, 2240, 2307
MaeII	ACGT	2 cut(s)	333, 991
MaeIII	GTNAC	5 cut(s)	523, 1279, 1725, 2060, 2331
MalI	GATC	7 cut(s)	430, 1038, 1113, 1379, 1960, 2189, 2379
MboI	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
MflI	RGATCY	2 cut(s)	428, 1958
MhlI	GDGCHC	2 cut(s)	1773, 1903
MlsI	TGGCCA	1 cut(s)	1448
MluNI	TGGCCA	1 cut(s)	1448
MlyI	GAGTC	7 cut(s)	74, 243, 1175, 1377, 1619, 2360, 2436
Mox20I	TGGCCA	1 cut(s)	1448
Mph1103I	ATGCAT	6 cut(s)	330, 781, 955, 1228, 1458, 2473
MroI	TCCGGA	1 cut(s)	1048
MroXI	GAANNNNTTC	2 cut(s)	1324, 1572
MscI	TGGCCA	1 cut(s)	1448
MseI	TTAA	5 cut(s)	408, 921, 1461, 2135, 2366
MslI	CAYNNNNRTG	2 cut(s)	2101, 2466
Msp20I	TGGCCA	1 cut(s)	1448
MspA1I	CMGCKG	2 cut(s)	501, 1346
MspI	CCGG	9 cut(s)	19, 240, 262, 422, 724, 1049, 1365, 2068, 2491
MspR9I	CCNGG	8 cut(s)	20, 262, 392, 423, 725, 830, 1467, 2154
Mva1269I	GAATGC	1 cut(s)	1555
MvaI	CCWGG	4 cut(s)	392, 830, 1467, 2154
MvnI	CGCG	1 cut(s)	356
MwoI	GCNNNNNNNGC	8 cut(s)	20, 264, 986, 1061, 2033, 2042, 2240, 2256
NciI	CCSGG	4 cut(s)	20, 262, 423, 725
NcoI	CCATGG	1 cut(s)	1418
NdeI	CATATG	1 cut(s)	1654
NdeII	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
NlaIV	GGNNCC	3 cut(s)	1617, 1900, 1960
NmuCI	GTSAC	1 cut(s)	523
NsiI	ATGCAT	6 cut(s)	330, 781, 955, 1228, 1458, 2473
NspI	RCATGY	6 cut(s)	524, 779, 1456, 1733, 1790, 2148
PaeI	GCATGC	2 cut(s)	1790, 2148
PceI	AGGCCT	1 cut(s)	1499
PciI	ACATGT	1 cut(s)	520
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1555
PdmI	GAANNNNTTC	2 cut(s)	1324, 1572
PfeI	GAWTC	3 cut(s)	282, 373, 403
PflMI	CCANNNNNTGG	1 cut(s)	880
PinAI	ACCGGT	1 cut(s)	2490
PleI	GAGTC	7 cut(s)	74, 242, 1175, 1377, 1619, 2359, 2435
PmaCI	CACGTG	1 cut(s)	992
PmlI	CACGTG	1 cut(s)	992
PpsI	GAGTC	7 cut(s)	74, 242, 1175, 1377, 1619, 2359, 2435
Ppu21I	YACGTR	1 cut(s)	992
PscI	ACATGT	1 cut(s)	520
PshAI	GACNNNNGTC	1 cut(s)	521
Psp6I	CCWGG	4 cut(s)	390, 828, 1465, 2152
PspCI	CACGTG	1 cut(s)	992
PspEI	GGTNACC	1 cut(s)	2331
PspFI	CCCAGC	1 cut(s)	2106
PspGI	CCWGG	4 cut(s)	390, 828, 1465, 2152
PspN4I	GGNNCC	3 cut(s)	1617, 1900, 1960
PspPI	GGNCC	2 cut(s)	1340, 1827
PsuI	RGATCY	2 cut(s)	428, 1958
PvuII	CAGCTG	1 cut(s)	1346
RsaI	GTAC	2 cut(s)	1617, 1908
RsaNI	GTAC	2 cut(s)	1616, 1907
RseI	CAYNNNNRTG	2 cut(s)	2101, 2466
SalI	GTCGAC	2 cut(s)	47, 77
SaqAI	TTAA	5 cut(s)	408, 921, 1461, 2135, 2366
Sau3AI	GATC	7 cut(s)	428, 1036, 1111, 1377, 1958, 2187, 2377
Sau96I	GGNCC	2 cut(s)	1340, 1827
SchI	GAGTC	7 cut(s)	74, 243, 1175, 1377, 1619, 2360, 2436
ScrFI	CCNGG	8 cut(s)	20, 262, 392, 423, 725, 830, 1467, 2154
SduI	GDGCHC	2 cut(s)	1773, 1903
SexAI	ACCWGGT	1 cut(s)	1465
SfcI	CTRYAG	1 cut(s)	1716
SinI	GGWCC	1 cut(s)	1340
SmiMI	CAYNNNNRTG	2 cut(s)	2101, 2466
SmlI	CTYRAG	2 cut(s)	697, 734
SmoI	CTYRAG	2 cut(s)	697, 734
SphI	GCATGC	2 cut(s)	1790, 2148
SseBI	AGGCCT	1 cut(s)	1499
SspI	AATATT	2 cut(s)	1521, 1711
SspMI	CTAG	7 cut(s)	44, 528, 812, 1766, 1880, 2240, 2307
StuI	AGGCCT	1 cut(s)	1499
StyD4I	CCNGG	8 cut(s)	18, 260, 390, 421, 723, 828, 1465, 2152
StyI	CCWWGG	3 cut(s)	207, 1418, 1980
TaaI	ACNGT	3 cut(s)	1640, 1717, 1725
TaiI	ACGT	2 cut(s)	336, 994
TaqI	TCGA	5 cut(s)	48, 78, 606, 840, 1400
TatI	WGTACW	1 cut(s)	1906
TauI	GCSGC	2 cut(s)	26, 762
TfiI	GAWTC	3 cut(s)	282, 373, 403
Tru1I	TTAA	5 cut(s)	408, 921, 1461, 2135, 2366
Tru9I	TTAA	5 cut(s)	408, 921, 1461, 2135, 2366
TscAI	CASTG	5 cut(s)	106, 460, 874, 946, 1339
TseFI	GTSAC	1 cut(s)	523
TseI	GCWGC	9 cut(s)	275, 488, 498, 1064, 1661, 1691, 1790, 2231, 2243
Tsp45I	GTSAC	1 cut(s)	523
TspDTI	ATGAA	7 cut(s)	85, 707, 1545, 1562, 1581, 1933, 2383
TspGWI	ACGGA	1 cut(s)	1519
TspRI	CASTG	5 cut(s)	106, 460, 874, 946, 1339
Van91I	CCANNNNNTGG	1 cut(s)	880
VpaK11BI	GGWCC	1 cut(s)	1340
XagI	CCTNNNNNAGG	1 cut(s)	1504
XapI	RAATTY	6 cut(s)	1413, 1532, 1568, 1994, 2157, 2441
XceI	RCATGY	6 cut(s)	524, 779, 1456, 1733, 1790, 2148
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1473
XmiI	GTMKAC	4 cut(s)	48, 78, 834, 1588
XmnI	GAANNNNTTC	2 cut(s)	1324, 1572
XspI	CTAG	7 cut(s)	44, 528, 812, 1766, 1880, 2240, 2307
Zsp2I	ATGCAT	6 cut(s)	330, 781, 955, 1228, 1458, 2473

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.