Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0005114

Physical Location & Seq

Genomic Coordinates Reverse (-)

13192818 .. 13202707

Gene Structure 9890 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG133100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGTGTACAAGGAGTTGCCAGGTGGATCTTCTTGGACATTGGATGTGTTCACTGAAGCATTTGTTGGAAAGTTGAAGTCATATCCTTATACTGCGATTAGCGGTCTGGAAATTAGTACACATGTCACTAGGAAAGATGTTGAGAAAGGTCTTTCTGAGGTTATACAGGCTGGAAAGCCTGTTCGCATATCAAATAAAAGTTTTATCGACTATATTTTCACTCGTAGTTTGGAGGTTGCTCTACTTTTTGACTTGCCAATGCAGATACACACAGGTTTTAGCAACAAAGATTTGAACTTGGGGCGTTCCGATCCCCTTCATCTCCGGGCTGTGCTTGAGGACAAGAGATTTTCTAAAAGCCGCATTGTTCTTTTACATGTATCCTACCCGTTTTCACAGGAAGCATCATATCTAGCCTCTGTTTATCCCCAGGTCTACCTCGACTTTGGCTGGGCTGTACCCAAGCTCAGTGTCCGCGGGATGGTATCTTCACTAAAAAAACTTTTGGAGCTTGCTCCAATTAAGAAGGTCATGTTCAGCACTGATGGATATGCATTTCCTGAAACTTTCTACATAGGTGCAAAAAAGGCACGTGACGCTGTCTTTTCAGTTCTATGTGATGCCTGTGCCGATGGTGATCTCTCAATTCCGGAAGCTATTGAGGCTGCTAAAGACATCTTTTCACAAAATGCAGTTCAGTTTTACAAGATTGATAGTGCTGTCAAATCTTCTGGTGCAGCCAATAGTGTATCCTCTAACTTTGAGAAGGTGAAGAGTAATGACTCGGAAAATCATGTCTCACTTGTTCGTGTGTTCTGGGCTGATGCATCAGGACAACAAAGATGTCGTGTTGTTCCTGCTAAGCGGTTCAATGACATCGTTGCAAAAAATGGTATTGGTTTGACTTTTGCTTGTATGGGAATGACTTCTTTCACTGACGGTCCAGCTGATGAGACTAACTTGACTGGAGTGGGTGAGATCAGACTCGTGCCTGATTTATCGACTAAACGGAGAATTCCATGGGTAGAACAAGAGGAAATGGTTTTGGCCAACATGCATCTTAAACCAGGTGAAGCATGGGAATACTGCCCAAGAGAGGCCTTACGGAGGGTTTCAAAAATATTGAAAGATGAATTCAATTTGGTAATGAATGCAGGATTTGAAAATGAATTTTTTCTCTTGAAGAGTATGCTAAGGGACGGGAAAGAAGAATGGGTACCATTTGACTCAACACCCTACTGTTCCCCATCATCATATGATGCTGCTTCCCCATTGTTTCACGAAGTTCTTGCTGCTCTCCAGTCCTTGAATATTTCTGTAGAACAGTTACATGCAGAAAGTGGGAAAGGTCAATTTGAGGTGGTTCTAGGGCACACAGCTTGCCAGCATGCTGCTGACAACTTGATTTATACCCGTGAAGTTATTAGGGCCATTGCAAGGAAACACGGATTGTTGGCAACCTTTGTCCCAAAGTATGCGCTAGATGAGATTGGCTCTGGAGCCCATGTACATCTCAGTTTGTGGCAGAATGGCAAAAATGTATTCATGGCATCTGGTGGATCCTCGCAGCATGGAATGTCCAAGGTTGGGGAGGAATTTATGGCAGGGGTTTTATATCATCTCCCAGCAATTTTGGCATTTACAGCTCCAATTCCAAATAGTTACGACCGGATACAACCTAATACTTGGAGTGGAGCATACAAGTGCTGGGGAAAAGAAAACAGGGAAGCTCCATTAAGAACTGCATGCCCACCTGGAGTTCAAGCTGGTATGGTGAGCAACTTTGAGATCAAGTCATTTGATGGGTGTGCAAATCCACACTTGGGCTTGGCTGCAATACTAGCTGCTGGCATTGATGGTCTTAGGAGACATCTTTGTCTCCCTGAACCTATTGATACAAATCCTTCTAGCCTTGAAGGAGAAATACAAAGGTTACCAACTTCACTTTTGGAGTCATTAGAAGCATTAAAGGAAGATGATCTCTTCAAAGAGTTCATTGGAGAAAAGCTATTGGTTGCCATCAAGGGAGTCCGCAAGGCAGAATTTGACTACTACGAGAAGCACAAAGATGCATACAAGCAACTTATACACCGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000905 GO:0001896 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009403 GO:0009404 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010720 GO:0010913 GO:0010914 GO:0012501 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019954 GO:0022414 GO:0030154 GO:0030436 GO:0030582 GO:0030584 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0034305 GO:0043385 GO:0043386 GO:0043455 GO:0043934 GO:0043937 GO:0043938 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0044550 GO:0045460 GO:0045461 GO:0045595 GO:0045597 GO:0046483 GO:0048315 GO:0048468 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0060284 GO:0061458 GO:0061794 GO:0065007 GO:0071704 GO:0075259 GO:0075306 GO:0075307 GO:1900376 GO:1900378 GO:1901360 GO:1901362 GO:1901376 GO:1901378 GO:1901576 GO:1903664 GO:1903666

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko00630 ko00910 ko01100 ko01120 ko01230 ko02020 ko04217 ko04724 ko04727 map00220 map00250 map00630 map00910 map01100 map01120 map01230 map02020 map04217 map04724 map04727

Pfam Domains

Protein Families

Amidohydro_2 Gln-synt_C Gln-synt_N_2

Protein Analysis

699

Amino Acids

77.52

Weight (kDa)

6.37

Isoelectric Point (pI)

39.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidohydro_2	PF04909	83 - 236	1.8e-12	Amidohydrolase
Gln-synt_N_2	PF16952	261 - 341	1.1e-07	Glutamine synthetase N-terminal domain
Gln-synt_C	PF00120	362 - 691	5.6e-92	Glutamine synthetase, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53180
fragaria_vesca	FvH4_6g16930 FvH4_6g16930 FvH4_6g16930
malus_domestica	MD00G1214800.v1.1 MD13G1277900.v1.1
prunus_persica	Prupe.7G124800_v2.0.a1 Prupe.7G124800_v2.0.a1
pyrus_communis	pycom04g08210
rosa_chinensis	RchiOBHm_Chr3g0470971 RchiOBHm_Chr5g0018301
rosa_laevigata	RLG00000024228
rosa_multiflora	Rmu_sc0005264.1_g000001 Rmu_sc0011919.1_g000005
rosa_roxburghii	Rroxscaffold_6G00410370
rosa_rugosa	Rorug03G0114300 Rorug03G0114300 Rorug03G0114400 Rorug05G0041200 Rorug05G0041200
rosa_samantha	Rh3AG164000 Rh3BG189600 Rh3CG179500 Rh3DG186600 Rh5AG134300 Rh5BG133100 Rh5CG144800 Rh5DG134500
rosa_wichuraiana	Rw3G015430 Rw5G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1875
Acc65I	GGTACC	1 cut(s)	1216
AccB1I	GGYRCC	1 cut(s)	1216
AccI	GTMKAC	1 cut(s)	435
AccII	CGCG	1 cut(s)	477
AccIII	TCCGGA	1 cut(s)	649
AciI	CCGC	7 cut(s)	102, 361, 475, 477, 865, 2032, 2092
AclWI	GGATC	4 cut(s)	34, 305, 1554, 1567
AcoI	YGGCCR	1 cut(s)	1047
AcsI	RAATTY	5 cut(s)	1014, 1133, 1169, 1595, 2042
AcuI	CTGAAG	1 cut(s)	75
AcvI	CACGTG	1 cut(s)	593
AfaI	GTAC	5 cut(s)	8, 118, 459, 1218, 1509
AfiI	CCNNNNNNNGG	6 cut(s)	481, 1096, 1107, 1437, 1587, 1823
AflIII	ACRYGT	2 cut(s)	121, 376
AjnI	CCWGG	4 cut(s)	19, 429, 1066, 1753
AjuI	GAANNNNNNNTTGG	2 cut(s)	48, 80
AloI	GAACNNNNNNTCC	2 cut(s)	165, 197
Alw26I	GTCTC	4 cut(s)	802, 947, 1861, 1883
AlwI	GGATC	4 cut(s)	34, 305, 1554, 1567
Aor13HI	TCCGGA	1 cut(s)	649
AoxI	GGCC	3 cut(s)	1047, 1098, 1428
ApeKI	GCWGC	8 cut(s)	665, 737, 1262, 1292, 1391, 1567, 1832, 1844
ApoI	RAATTY	5 cut(s)	1014, 1133, 1169, 1595, 2042
Asp700I	GAANNNNTTC	2 cut(s)	925, 1173
Asp718I	GGTACC	1 cut(s)	1216
AspLEI	GCGC	1 cut(s)	1480
AspS9I	GGNCC	2 cut(s)	941, 1428
AsuC2I	CCSGG	1 cut(s)	326
AsuHPI	GGTGA	5 cut(s)	647, 781, 986, 1082, 1786
AvaII	GGWCC	1 cut(s)	941
BaeGI	GKGCMC	1 cut(s)	1374
BalI	TGGCCA	1 cut(s)	1049
BamHI	GGATCC	1 cut(s)	1559
BanI	GGYRCC	1 cut(s)	1216
BanII	GRGCYC	1 cut(s)	1504
BarI	GAAGNNNNNNTAC	4 cut(s)	1200, 1232, 1908, 1940
BauI	CACGAG	1 cut(s)	986
BbrPI	CACGTG	1 cut(s)	593
BbvI	GCAGC	8 cut(s)	652, 749, 1249, 1279, 1378, 1579, 1819, 1831
BccI	CCATC	7 cut(s)	475, 539, 626, 1255, 1796, 1850, 2027
BcgI	CGANNNNNNTGC	2 cut(s)	1271, 1305
BciT130I	CCWGG	4 cut(s)	21, 431, 1068, 1755
BciVI	GTATCC	3 cut(s)	391, 760, 1665
BcnI	CCSGG	1 cut(s)	326
BcoDI	GTCTC	4 cut(s)	802, 947, 1861, 1883
BfaI	CTAG	6 cut(s)	129, 413, 1367, 1481, 1841, 1908
BfmI	CTRYAG	1 cut(s)	1317
BfuI	GTATCC	3 cut(s)	391, 760, 1665
BisI	GCNGC	9 cut(s)	361, 666, 738, 1263, 1293, 1392, 1568, 1833, 1845
BlpI	GCTNAGC	1 cut(s)	861
BlsI	GCNGC	9 cut(s)	362, 667, 739, 1264, 1294, 1393, 1569, 1834, 1846
Bme1390I	CCNGG	5 cut(s)	21, 326, 431, 1068, 1755
Bme18I	GGWCC	1 cut(s)	941
BmgT120I	GGNCC	2 cut(s)	941, 1428
BmiI	GGNNCC	3 cut(s)	1218, 1501, 1561
BmrFI	CCNGG	5 cut(s)	21, 326, 431, 1068, 1755
BmsI	GCATC	8 cut(s)	413, 610, 814, 836, 1066, 1249, 1559, 2059
BplI	GAGNNNNNCTC	2 cut(s)	1478, 1510
BpmI	CTGGAG	4 cut(s)	987, 1283, 1518, 1776
Bpu10I	CCTNAGC	1 cut(s)	1193
Bpu1102I	GCTNAGC	1 cut(s)	861
BpuEI	CTTGAG	1 cut(s)	356
BpuMI	CCSGG	1 cut(s)	326
BsaAI	YACGTR	1 cut(s)	593
BsaBI	GATNNNNATC	2 cut(s)	636, 1899
BsaJI	CCNNGG	4 cut(s)	429, 475, 1019, 1581
BsaWI	WCCGGW	2 cut(s)	649, 1668
BsaXI	ACNNNNNCTCC	2 cut(s)	1491, 1521
Bsc4I	CCNNNNNNNGG	6 cut(s)	481, 1096, 1107, 1437, 1587, 1823
Bse1I	ACTGG	2 cut(s)	970, 1300
Bse3DI	GCAATG	1 cut(s)	1431
Bse8I	GATNNNNATC	2 cut(s)	636, 1899
BseAI	TCCGGA	1 cut(s)	649
BseBI	CCWGG	4 cut(s)	21, 431, 1068, 1755
BseDI	CCNNGG	4 cut(s)	429, 475, 1019, 1581
BseGI	GGATG	2 cut(s)	49, 486
BseJI	GATNNNNATC	2 cut(s)	636, 1899
BseLI	CCNNNNNNNGG	6 cut(s)	481, 1096, 1107, 1437, 1587, 1823
BseMI	GCAATG	1 cut(s)	1431
BseMII	CTCAG	3 cut(s)	147, 481, 1528
BseNI	ACTGG	2 cut(s)	970, 1300
BseSI	GKGCMC	1 cut(s)	1374
BseXI	GCAGC	8 cut(s)	652, 749, 1249, 1279, 1378, 1579, 1819, 1831
BseYI	CCCAGC	3 cut(s)	450, 1624, 1707
BsgI	GTGCAG	1 cut(s)	756
Bsh1236I	CGCG	1 cut(s)	477
Bsh1285I	CGRYCG	1 cut(s)	1669
BshFI	GGCC	3 cut(s)	1049, 1100, 1430
BshNI	GGYRCC	1 cut(s)	1216
BsiEI	CGRYCG	1 cut(s)	1669
BsiSI	CCGG	3 cut(s)	325, 650, 1669
BslFI	GGGAC	2 cut(s)	1211, 1451
BslI	CCNNNNNNNGG	6 cut(s)	481, 1096, 1107, 1437, 1587, 1823
BsmAI	GTCTC	4 cut(s)	802, 947, 1861, 1883
BsmFI	GGGAC	2 cut(s)	1211, 1451
BsmI	GAATGC	1 cut(s)	1156
BsnI	GGCC	3 cut(s)	1049, 1100, 1430
Bsp1286I	GDGCHC	2 cut(s)	1374, 1504
Bsp13I	TCCGGA	1 cut(s)	649
Bsp1407I	TGTACA	2 cut(s)	6, 1507
Bsp143I	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
Bsp1720I	GCTNAGC	1 cut(s)	861
Bsp19I	CCATGG	1 cut(s)	1019
BspACI	CCGC	7 cut(s)	102, 361, 475, 477, 865, 2032, 2092
BspANI	GGCC	3 cut(s)	1049, 1100, 1430
BspCNI	CTCAG	3 cut(s)	148, 480, 1527
BspEI	TCCGGA	1 cut(s)	649
BspFNI	CGCG	1 cut(s)	477
BspLI	GGNNCC	3 cut(s)	1218, 1501, 1561
BspPI	GGATC	4 cut(s)	34, 305, 1554, 1567
BspT107I	GGYRCC	1 cut(s)	1216
BsrDI	GCAATG	1 cut(s)	1431
BsrGI	TGTACA	2 cut(s)	6, 1507
BsrI	ACTGG	2 cut(s)	970, 1300
BssECI	CCNNGG	4 cut(s)	429, 475, 1019, 1581
BssMI	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
BssSI	CACGAG	1 cut(s)	986
BssT1I	CCWWGG	2 cut(s)	1019, 1581
Bst2BI	CACGAG	1 cut(s)	986
Bst2UI	CCWGG	4 cut(s)	21, 431, 1068, 1755
Bst4CI	ACNGT	3 cut(s)	941, 1241, 1326
Bst6I	CTCTTC	3 cut(s)	767, 1178, 1988
BstAUI	TGTACA	2 cut(s)	6, 1507
BstBAI	YACGTR	1 cut(s)	593
BstC8I	GCNNGC	6 cut(s)	513, 1381, 1385, 1389, 1747, 1849
BstDEI	CTNAG	6 cut(s)	156, 467, 861, 1193, 1514, 1862
BstDSI	CCRYGG	2 cut(s)	475, 1019
BstEII	GGTNACC	1 cut(s)	1932
BstENI	CCTNNNNNAGG	1 cut(s)	1105
BstF5I	GGATG	2 cut(s)	49, 486
BstFNI	CGCG	1 cut(s)	477
BstHHI	GCGC	1 cut(s)	1480
BstKTI	GATC	7 cut(s)	29, 313, 640, 981, 1562, 1791, 1981
BstMAI	GTCTC	4 cut(s)	802, 947, 1861, 1883
BstMBI	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
BstMCI	CGRYCG	1 cut(s)	1669
BstMWI	GCNNNNNNNGC	6 cut(s)	587, 596, 662, 1634, 1643, 1841
BstNI	CCWGG	4 cut(s)	21, 431, 1068, 1755
BstNSI	RCATGY	6 cut(s)	125, 380, 1057, 1334, 1391, 1749
BstPI	GGTNACC	1 cut(s)	1932
BstSCI	CCNGG	5 cut(s)	19, 324, 429, 1066, 1753
BstSFI	CTRYAG	1 cut(s)	1317
BstSLI	GKGCMC	1 cut(s)	1374
BstUI	CGCG	1 cut(s)	477
BstV1I	GCAGC	8 cut(s)	652, 749, 1249, 1279, 1378, 1579, 1819, 1831
BstX2I	RGATCY	2 cut(s)	26, 1559
BstYI	RGATCY	2 cut(s)	26, 1559
BsuI	GTATCC	3 cut(s)	391, 760, 1665
BsuRI	GGCC	3 cut(s)	1049, 1100, 1430
BtgI	CCRYGG	2 cut(s)	475, 1019
BtsCI	GGATG	2 cut(s)	49, 486
BtsIMutI	CAGTG	4 cut(s)	51, 475, 540, 933
Cac8I	GCNNGC	6 cut(s)	513, 1381, 1385, 1389, 1747, 1849
CfoI	GCGC	1 cut(s)	1480
Cfr13I	GGNCC	2 cut(s)	941, 1428
Cfr42I	CCGCGG	1 cut(s)	478
CseI	GACGC	1 cut(s)	605
CsiI	ACCWGGT	1 cut(s)	1066
Csp6I	GTAC	5 cut(s)	7, 117, 458, 1217, 1508
CviQI	GTAC	5 cut(s)	7, 117, 458, 1217, 1508
DdeI	CTNAG	6 cut(s)	156, 467, 861, 1193, 1514, 1862
DpnI	GATC	7 cut(s)	28, 312, 639, 980, 1561, 1790, 1980
DpnII	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
DrdI	GACNNNNNNGTC	1 cut(s)	1875
DseDI	GACNNNNNNGTC	1 cut(s)	1875
EaeI	YGGCCR	1 cut(s)	1047
Eam1104I	CTCTTC	3 cut(s)	767, 1178, 1988
EarI	CTCTTC	3 cut(s)	767, 1178, 1988
Eco130I	CCWWGG	2 cut(s)	1019, 1581
Eco147I	AGGCCT	1 cut(s)	1100
Eco24I	GRGCYC	1 cut(s)	1504
Eco47I	GGWCC	1 cut(s)	941
Eco57I	CTGAAG	1 cut(s)	75
Eco72I	CACGTG	1 cut(s)	593
Eco91I	GGTNACC	1 cut(s)	1932
EcoNI	CCTNNNNNAGG	1 cut(s)	1105
EcoO65I	GGTNACC	1 cut(s)	1932
EcoRI	GAATTC	2 cut(s)	1014, 1133
EcoRII	CCWGG	4 cut(s)	19, 429, 1066, 1753
EcoT14I	CCWWGG	2 cut(s)	1019, 1581
EcoT22I	ATGCAT	4 cut(s)	556, 829, 1059, 2074
EcoT38I	GRGCYC	1 cut(s)	1504
ErhI	CCWWGG	2 cut(s)	1019, 1581
FaqI	GGGAC	2 cut(s)	1211, 1451
FauI	CCCGC	1 cut(s)	470
FauNDI	CATATG	1 cut(s)	1255
FblI	GTMKAC	1 cut(s)	435
Fnu4HI	GCNGC	9 cut(s)	361, 666, 738, 1263, 1293, 1392, 1568, 1833, 1845
FokI	GGATG	2 cut(s)	56, 493
FriOI	GRGCYC	1 cut(s)	1504
Fsp4HI	GCNGC	9 cut(s)	361, 666, 738, 1263, 1293, 1392, 1568, 1833, 1845
FspBI	CTAG	6 cut(s)	129, 413, 1367, 1481, 1841, 1908
GlaI	GCGC	1 cut(s)	1479
GluI	GCNGC	9 cut(s)	361, 666, 738, 1263, 1293, 1392, 1568, 1833, 1845
GsaI	CCCAGC	3 cut(s)	454, 1628, 1711
GsuI	CTGGAG	4 cut(s)	987, 1283, 1518, 1776
HaeIII	GGCC	3 cut(s)	1049, 1100, 1430
HapII	CCGG	3 cut(s)	325, 650, 1669
HgaI	GACGC	1 cut(s)	605
HhaI	GCGC	1 cut(s)	1480
Hin6I	GCGC	1 cut(s)	1478
HinP1I	GCGC	1 cut(s)	1478
HinfI	GANTC	5 cut(s)	782, 984, 1226, 1952, 2028
HpaII	CCGG	3 cut(s)	325, 650, 1669
HphI	GGTGA	5 cut(s)	647, 781, 986, 1082, 1786
Hpy166II	GTNNAC	4 cut(s)	7, 51, 119, 436
Hpy188I	TCNGA	4 cut(s)	157, 310, 787, 983
Hpy188III	TCNNGA	7 cut(s)	107, 560, 650, 831, 1180, 1280, 1497
Hpy8I	GTNNAC	4 cut(s)	7, 51, 119, 436
HpyAV	CCTTC	5 cut(s)	326, 520, 760, 1910, 1914
HpyCH4III	ACNGT	3 cut(s)	941, 1241, 1326
HpyCH4IV	ACGT	1 cut(s)	592
HpyF10VI	GCNNNNNNNGC	6 cut(s)	587, 596, 662, 1634, 1643, 1841
HpyF3I	CTNAG	6 cut(s)	156, 467, 861, 1193, 1514, 1862
HpySE526I	ACGT	1 cut(s)	592
HspAI	GCGC	1 cut(s)	1478
Kpn2I	TCCGGA	1 cut(s)	649
KpnI	GGTACC	1 cut(s)	1220
KspI	CCGCGG	1 cut(s)	478
Kzo9I	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
LmnI	GCTCC	6 cut(s)	508, 520, 1499, 1651, 1694, 1735
Lsp1109I	GCAGC	8 cut(s)	652, 749, 1249, 1279, 1378, 1579, 1819, 1831
LweI	GCATC	8 cut(s)	413, 610, 814, 836, 1066, 1249, 1559, 2059
MabI	ACCWGGT	1 cut(s)	1066
MaeI	CTAG	6 cut(s)	129, 413, 1367, 1481, 1841, 1908
MaeII	ACGT	1 cut(s)	592
MaeIII	GTNAC	5 cut(s)	124, 593, 1326, 1661, 1932
MalI	GATC	7 cut(s)	28, 312, 639, 980, 1561, 1790, 1980
MboI	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
MboII	GAAGA	8 cut(s)	21, 480, 720, 784, 1195, 1220, 1975, 1985
MflI	RGATCY	2 cut(s)	26, 1559
MhlI	GDGCHC	2 cut(s)	1374, 1504
MlsI	TGGCCA	1 cut(s)	1049
MluNI	TGGCCA	1 cut(s)	1049
MlyI	GAGTC	5 cut(s)	776, 978, 1220, 1961, 2037
MmeI	TCCRAC	1 cut(s)	46
Mox20I	TGGCCA	1 cut(s)	1049
Mph1103I	ATGCAT	4 cut(s)	556, 829, 1059, 2074
MroI	TCCGGA	1 cut(s)	649
MroXI	GAANNNNTTC	2 cut(s)	925, 1173
MscI	TGGCCA	1 cut(s)	1049
MseI	TTAA	4 cut(s)	522, 1062, 1736, 1967
MslI	CAYNNNNRTG	2 cut(s)	1702, 2067
Msp20I	TGGCCA	1 cut(s)	1049
MspA1I	CMGCKG	2 cut(s)	477, 947
MspI	CCGG	3 cut(s)	325, 650, 1669
MspR9I	CCNGG	5 cut(s)	21, 326, 431, 1068, 1755
Mva1269I	GAATGC	1 cut(s)	1156
MvaI	CCWGG	4 cut(s)	21, 431, 1068, 1755
MvnI	CGCG	1 cut(s)	477
MwoI	GCNNNNNNNGC	6 cut(s)	587, 596, 662, 1634, 1643, 1841
NciI	CCSGG	1 cut(s)	326
NcoI	CCATGG	1 cut(s)	1019
NdeI	CATATG	1 cut(s)	1255
NdeII	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
NlaIV	GGNNCC	3 cut(s)	1218, 1501, 1561
NmuCI	GTSAC	2 cut(s)	124, 593
NsiI	ATGCAT	4 cut(s)	556, 829, 1059, 2074
NspI	RCATGY	6 cut(s)	125, 380, 1057, 1334, 1391, 1749
PaeI	GCATGC	2 cut(s)	1391, 1749
PceI	AGGCCT	1 cut(s)	1100
PciI	ACATGT	2 cut(s)	121, 376
PctI	GAATGC	1 cut(s)	1156
PdmI	GAANNNNTTC	2 cut(s)	925, 1173
PflFI	GACNNNGTC	1 cut(s)	599
PkrI	GCNGC	9 cut(s)	362, 667, 739, 1264, 1294, 1393, 1569, 1834, 1846
PleI	GAGTC	5 cut(s)	776, 978, 1220, 1960, 2036
PmaCI	CACGTG	1 cut(s)	593
PmlI	CACGTG	1 cut(s)	593
PpsI	GAGTC	5 cut(s)	776, 978, 1220, 1960, 2036
Ppu21I	YACGTR	1 cut(s)	593
PscI	ACATGT	2 cut(s)	121, 376
Psp6I	CCWGG	4 cut(s)	19, 429, 1066, 1753
PspCI	CACGTG	1 cut(s)	593
PspEI	GGTNACC	1 cut(s)	1932
PspFI	CCCAGC	3 cut(s)	450, 1624, 1707
PspGI	CCWGG	4 cut(s)	19, 429, 1066, 1753
PspN4I	GGNNCC	3 cut(s)	1218, 1501, 1561
PspPI	GGNCC	2 cut(s)	941, 1428
PsuI	RGATCY	2 cut(s)	26, 1559
PsyI	GACNNNGTC	1 cut(s)	599
PvuII	CAGCTG	1 cut(s)	947
RsaI	GTAC	5 cut(s)	8, 118, 459, 1218, 1509
RsaNI	GTAC	5 cut(s)	7, 117, 458, 1217, 1508
RseI	CAYNNNNRTG	2 cut(s)	1702, 2067
SacII	CCGCGG	1 cut(s)	478
SaqAI	TTAA	4 cut(s)	522, 1062, 1736, 1967
SatI	GCNGC	9 cut(s)	361, 666, 738, 1263, 1293, 1392, 1568, 1833, 1845
Sau3AI	GATC	7 cut(s)	26, 310, 637, 978, 1559, 1788, 1978
Sau96I	GGNCC	2 cut(s)	941, 1428
SchI	GAGTC	5 cut(s)	776, 978, 1220, 1961, 2037
ScrFI	CCNGG	5 cut(s)	21, 326, 431, 1068, 1755
SduI	GDGCHC	2 cut(s)	1374, 1504
SexAI	ACCWGGT	1 cut(s)	1066
SfaNI	GCATC	8 cut(s)	413, 610, 814, 836, 1066, 1249, 1559, 2059
SfcI	CTRYAG	1 cut(s)	1317
Sfr303I	CCGCGG	1 cut(s)	478
SgrBI	CCGCGG	1 cut(s)	478
SinI	GGWCC	1 cut(s)	941
SmiMI	CAYNNNNRTG	2 cut(s)	1702, 2067
SmlI	CTYRAG	1 cut(s)	335
SmoI	CTYRAG	1 cut(s)	335
SphI	GCATGC	2 cut(s)	1391, 1749
SseBI	AGGCCT	1 cut(s)	1100
SsiI	CCGC	7 cut(s)	102, 361, 475, 477, 865, 2032, 2092
SspI	AATATT	2 cut(s)	1122, 1312
SspMI	CTAG	6 cut(s)	129, 413, 1367, 1481, 1841, 1908
StuI	AGGCCT	1 cut(s)	1100
StyD4I	CCNGG	5 cut(s)	19, 324, 429, 1066, 1753
StyI	CCWWGG	2 cut(s)	1019, 1581
TaaI	ACNGT	3 cut(s)	941, 1241, 1326
TaiI	ACGT	1 cut(s)	595
TaqI	TCGA	3 cut(s)	207, 441, 1001
TatI	WGTACW	3 cut(s)	6, 116, 1507
TauI	GCSGC	1 cut(s)	363
Tru1I	TTAA	4 cut(s)	522, 1062, 1736, 1967
Tru9I	TTAA	4 cut(s)	522, 1062, 1736, 1967
TscAI	CASTG	4 cut(s)	58, 475, 547, 940
TseFI	GTSAC	2 cut(s)	124, 593
TseI	GCWGC	8 cut(s)	665, 737, 1262, 1292, 1391, 1567, 1832, 1844
Tsp45I	GTSAC	2 cut(s)	124, 593
TspDTI	ATGAA	6 cut(s)	308, 1146, 1163, 1182, 1534, 1984
TspGWI	ACGGA	3 cut(s)	1024, 1120, 1461
TspRI	CASTG	4 cut(s)	58, 475, 547, 940
Tth111I	GACNNNGTC	1 cut(s)	599
VpaK11BI	GGWCC	1 cut(s)	941
XagI	CCTNNNNNAGG	1 cut(s)	1105
XapI	RAATTY	5 cut(s)	1014, 1133, 1169, 1595, 2042
XceI	RCATGY	6 cut(s)	125, 380, 1057, 1334, 1391, 1749
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1074
XmiI	GTMKAC	1 cut(s)	435
XmnI	GAANNNNTTC	2 cut(s)	925, 1173
XspI	CTAG	6 cut(s)	129, 413, 1367, 1481, 1841, 1908
Zsp2I	ATGCAT	4 cut(s)	556, 829, 1059, 2074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.