Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001966

Physical Location & Seq

Genomic Coordinates Reverse (-)

20311901 .. 20315887

Gene Structure 3987 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G54830.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGAATCACTCCACTTCTGACCAGAGCCTCTACATCGAGAGTGATGATGGAGATGACGAGAGAAAACACTTGTCCGACGATGAAGACGACGATGGAACTCTTTCTGATACTTCTGACGCTTACAATCAAAACCAACATCATCTGTCTAAAGCTAGCCCATACTCAACGGCATGGCCTAAGAGTTATAGGCAATCGATTGATATGTTTGGAAGTGTACCATCACCAAATTTGGGTTTTCTTGCTAATTCTTCTATGTCGAGAAGAGGAAGCTCATTCATGTCTTCGACACTGACAAGAAGACATACTCCAGAATCATTACCTTGTGTTACAAAGCCTCTCCTAGAAGATGAAGAAGCACCAAAACATAAACTTAGCACTCATTCTTTGCTTCCGTCGAAACCATCGTCATCCATGGTGGTGTCTCATGATATGGGAATCTCCAATGATAGCTCCTTTGGACAGGCTGTTCTTAATGGAGTGAATGTTTTATGTGGGGTTGGAATATTGTCAACACCATATGCTGTGAAGGAAGGAGGATGGTTGGGACTTATCATTCTCTTTGCATTTGGTATTCTTTGTTTTTACACCGGTTTGCTTCTGCGGTACTGCCTCGACAGCCACCCCGATGTCCAGACCTATCCTGATATTGGCCATGCCGCTTTTGGTTCCACCGGCCGCATCCTGGTCTCCGTAATACTCTATATGGAGTTATATGCTATGAGTGTTGAATACATAATATTGGAAGGTGATAATCTTTCATCAATGTTTCCAAATGCTAGTCTAAGTATTGGAGGGTTTCACTTAGATGCGCCTCGTCTATTTGCTCTGCTTACCACCCTCGCTGTTCTCCCAACCGTTTGGCTTCGAGATCTCAGCGTACTAAGTTACATTTCAGCTGGAGGGGTGATTGCATCAGTGTTGGTGGTTTTGTGCTTGTTTTGGGTTGGTTTGGTTGATGATGTTGGTATTCACAGCAAAGGAACTCCACTAAACCTCGCAACATTACCGGTTTCTGTAGGACTCTATGGTTATTGTTACTCAGGACATGGTGTTTTCCCTAATATTTACACTTCAATGGCCAAACCATCTCAATTTTCTGCTGTTCTCTTGGCATCTTTTGGAATTTGTACTCTTATGTATGCGGGTGTTGCTGTTATGGGATATAGCATGTTTGGAGAATCAACAGAATCACAGTTTACTCTTAACTTGCCTCAAGATTTGGTTGCATCTAAGATTGCTTTGTGGACTACAGTTGTTAATCCATTTACCAAATATCCTTTATCAAGAAATATTCAGAGACTTTTCATTTATCTCTTTGCAATGAGTCTTGAGGAGTTAATACCATCAAACTATGGGAAGTCACGTTTCTACGCAATCGCCATTAGAAGTGCATTAGCCATCTCTACTCTGCTCGTTGGTCTTGCGATTCCCTTCTTCGGCCTTGTCATGTCACTGATTGGATCATTCTTGACAATGCTCATTTTTCTGGACACAGACGCTGATACTACCGCCAGCTTGTTTCTTGAGCATCTTAAGGAAAAAAGTAACCCCTACTCAGGTGACAATCTGTATCTTAATTATGACAGTAGGAGCAGTATGTTCAGTCATTGGCACATATTCAGCCCTTGCAAAGATCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0098588 GO:0098656 GO:0098805 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

546

Amino Acids

59.74

Weight (kDa)

5.36

Isoelectric Point (pI)

45.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	150 - 494	4.8e-59	Transmembrane amino acid transporter protein
Trp_Tyr_perm	PF03222	162 - 361	4.8e-06	Tryptophan/tyrosine permease family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001966)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39130 AT2G39130 AT2G39130 AT3G54830 AT3G54830
fragaria_vesca	FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870
malus_domestica	MD01G1104600.v1.1 MD01G1104700.v1.1 MD07G1171200.v1.1
prunus_persica	Prupe.2G209900_v2.0.a1 Prupe.2G209900_v2.0.a1 Prupe.2G209900_v2.0.a1 Prupe.2G209900_v2.0.a1
pyrus_communis	pycom01g13120 pycom01g13130 pycom07g16500
rosa_chinensis	RchiOBHm_Chr1g0362811
rosa_laevigata	RLG00000027620
rosa_multiflora	Rmu_sc0012836.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293750
rosa_rugosa	Rorug01G0301400 Rorug01G0301500 Rorug01G0301600 Rorug01G0301700
rosa_samantha	Rh1AG310400 Rh1BG273600 Rh1CG290800 Rh1DG304300
rosa_wichuraiana	Rw1G027520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	5 cut(s)	601, 657, 676, 1142, 1509
AclWI	GGATC	1 cut(s)	1468
AcoI	YGGCCR	3 cut(s)	649, 673, 1077
AcsI	RAATTY	2 cut(s)	226, 1122
AfaI	GTAC	4 cut(s)	216, 605, 879, 1129
AfiI	CCNNNNNNNGG	5 cut(s)	230, 647, 682, 1436, 1556
AflII	CTTAAG	1 cut(s)	1532
AgeI	ACCGGT	2 cut(s)	587, 1006
AgsI	TTSAA	2 cut(s)	728, 1074
AjnI	CCWGG	1 cut(s)	681
AloI	GAACNNNNNNTCC	2 cut(s)	450, 482
AluBI	AGCT	5 cut(s)	152, 270, 450, 896, 1515
AluI	AGCT	5 cut(s)	152, 270, 450, 896, 1515
Alw26I	GTCTC	3 cut(s)	426, 691, 1291
AlwI	GGATC	1 cut(s)	1468
AlwNI	CAGNNNCTG	1 cut(s)	1499
AoxI	GGCC	5 cut(s)	173, 649, 673, 1077, 1438
ApoI	RAATTY	2 cut(s)	226, 1122
ArsI	GACNNNNNNTTYG	2 cut(s)	389, 421
AsiGI	ACCGGT	2 cut(s)	587, 1006
Asp700I	GAANNNNTTC	1 cut(s)	100
AspLEI	GCGC	1 cut(s)	811
AsuHPI	GGTGA	4 cut(s)	213, 758, 916, 1571
AsuNHI	GCTAGC	1 cut(s)	152
BalI	TGGCCA	2 cut(s)	651, 1079
BbsI	GAAGAC	3 cut(s)	90, 273, 304
BccI	CCATC	8 cut(s)	41, 86, 226, 409, 531, 1093, 1351, 1406
BceAI	ACGGC	1 cut(s)	183
BciT130I	CCWGG	1 cut(s)	683
BcoDI	GTCTC	3 cut(s)	426, 691, 1291
BfaI	CTAG	3 cut(s)	153, 341, 777
BfmI	CTRYAG	2 cut(s)	1014, 1248
BfrI	CTTAAG	1 cut(s)	1532
BglII	AGATCT	1 cut(s)	868
BisI	GCNGC	2 cut(s)	657, 676
BlsI	GCNGC	2 cut(s)	658, 677
Bme1390I	CCNGG	1 cut(s)	683
BmiI	GGNNCC	1 cut(s)	667
BmrFI	CCNGG	1 cut(s)	683
BmsI	GCATC	6 cut(s)	687, 796, 920, 1121, 1235, 1537
BmtI	GCTAGC	1 cut(s)	156
BpiI	GAAGAC	3 cut(s)	90, 273, 304
BpmI	CTGGAG	2 cut(s)	291, 918
BpuEI	CTTGAG	3 cut(s)	1197, 1349, 1544
Bsa29I	ATCGAT	1 cut(s)	194
BsaI	GGTCTC	1 cut(s)	691
BsaJI	CCNNGG	1 cut(s)	411
BsaWI	WCCGGW	2 cut(s)	587, 1006
BsaXI	ACNNNNNCTCC	2 cut(s)	468, 498
Bsc4I	CCNNNNNNNGG	5 cut(s)	230, 647, 682, 1436, 1556
Bse118I	RCCGGY	3 cut(s)	587, 671, 1006
Bse3DI	GCAATG	1 cut(s)	1326
BseBI	CCWGG	1 cut(s)	683
BseCI	ATCGAT	1 cut(s)	194
BseDI	CCNNGG	1 cut(s)	411
BseGI	GGATG	3 cut(s)	407, 542, 678
BseLI	CCNNNNNNNGG	5 cut(s)	230, 647, 682, 1436, 1556
BseMI	GCAATG	1 cut(s)	1326
BseMII	CTCAG	3 cut(s)	886, 1053, 1569
BseRI	GAGGAG	1 cut(s)	1346
BseX3I	CGGCCG	1 cut(s)	673
Bsh1285I	CGRYCG	1 cut(s)	676
BshFI	GGCC	5 cut(s)	175, 651, 675, 1079, 1440
BshTI	ACCGGT	2 cut(s)	587, 1006
BshVI	ATCGAT	1 cut(s)	194
BsiEI	CGRYCG	1 cut(s)	676
BsiSI	CCGG	3 cut(s)	588, 672, 1007
BslFI	GGGAC	1 cut(s)	558
BslI	CCNNNNNNNGG	5 cut(s)	230, 647, 682, 1436, 1556
BsmAI	GTCTC	3 cut(s)	426, 691, 1291
BsmFI	GGGAC	1 cut(s)	558
BsnI	GGCC	5 cut(s)	175, 651, 675, 1079, 1440
Bso31I	GGTCTC	1 cut(s)	691
Bsp143I	GATC	3 cut(s)	868, 1460, 1633
Bsp19I	CCATGG	1 cut(s)	411
BspACI	CCGC	5 cut(s)	601, 657, 676, 1142, 1509
BspANI	GGCC	5 cut(s)	175, 651, 675, 1079, 1440
BspCNI	CTCAG	3 cut(s)	885, 1052, 1568
BspDI	ATCGAT	1 cut(s)	194
BspHI	TCATGA	1 cut(s)	424
BspLI	GGNNCC	1 cut(s)	667
BspOI	GCTAGC	1 cut(s)	156
BspPI	GGATC	1 cut(s)	1468
BspTI	CTTAAG	1 cut(s)	1532
BspTNI	GGTCTC	1 cut(s)	691
BsrDI	GCAATG	1 cut(s)	1326
BsrFI	RCCGGY	3 cut(s)	587, 671, 1006
BssAI	RCCGGY	3 cut(s)	587, 671, 1006
BssECI	CCNNGG	1 cut(s)	411
BssMI	GATC	3 cut(s)	868, 1460, 1633
BssT1I	CCWWGG	1 cut(s)	411
Bst2UI	CCWGG	1 cut(s)	683
Bst4CI	ACNGT	4 cut(s)	856, 1194, 1252, 1586
Bst6I	CTCTTC	1 cut(s)	256
BstAFI	CTTAAG	1 cut(s)	1532
BstC8I	GCNNGC	2 cut(s)	154, 1513
BstDEI	CTNAG	9 cut(s)	177, 371, 782, 802, 872, 881, 1039, 1230, 1555
BstDSI	CCRYGG	1 cut(s)	411
BstF5I	GGATG	3 cut(s)	407, 542, 678
BstHHI	GCGC	1 cut(s)	811
BstKTI	GATC	3 cut(s)	871, 1463, 1636
BstMAI	GTCTC	3 cut(s)	426, 691, 1291
BstMBI	GATC	3 cut(s)	868, 1460, 1633
BstMCI	CGRYCG	1 cut(s)	676
BstMWI	GCNNNNNNNGC	2 cut(s)	615, 1148
BstNI	CCWGG	1 cut(s)	683
BstNSI	RCATGY	1 cut(s)	1171
BstSCI	CCNGG	1 cut(s)	681
BstSFI	CTRYAG	2 cut(s)	1014, 1248
BstV2I	GAAGAC	3 cut(s)	90, 273, 304
BstX2I	RGATCY	1 cut(s)	868
BstXI	CCANNNNNNTGG	1 cut(s)	858
BstYI	RGATCY	1 cut(s)	868
BstZI	CGGCCG	1 cut(s)	673
Bsu15I	ATCGAT	1 cut(s)	194
BsuRI	GGCC	5 cut(s)	175, 651, 675, 1079, 1440
BsuTUI	ATCGAT	1 cut(s)	194
BtgI	CCRYGG	1 cut(s)	411
BtsCI	GGATG	3 cut(s)	407, 542, 678
BtsIMutI	CAGTG	3 cut(s)	287, 921, 1451
Cac8I	GCNNGC	2 cut(s)	154, 1513
CaiI	CAGNNNCTG	1 cut(s)	1499
CciI	TCATGA	1 cut(s)	424
CfoI	GCGC	1 cut(s)	811
Cfr10I	RCCGGY	3 cut(s)	587, 671, 1006
ClaI	ATCGAT	1 cut(s)	194
CseI	GACGC	2 cut(s)	125, 1505
Csp6I	GTAC	4 cut(s)	215, 604, 878, 1128
CspAI	ACCGGT	2 cut(s)	587, 1006
CviAII	CATG	8 cut(s)	171, 277, 412, 425, 653, 1046, 1168, 1447
CviQI	GTAC	4 cut(s)	215, 604, 878, 1128
DdeI	CTNAG	9 cut(s)	177, 371, 782, 802, 872, 881, 1039, 1230, 1555
DpnI	GATC	3 cut(s)	870, 1462, 1635
DpnII	GATC	3 cut(s)	868, 1460, 1633
EaeI	YGGCCR	3 cut(s)	649, 673, 1077
EagI	CGGCCG	1 cut(s)	673
Eam1104I	CTCTTC	1 cut(s)	256
EarI	CTCTTC	1 cut(s)	256
EclXI	CGGCCG	1 cut(s)	673
Eco130I	CCWWGG	1 cut(s)	411
Eco31I	GGTCTC	1 cut(s)	691
Eco52I	CGGCCG	1 cut(s)	673
EcoRII	CCWGG	1 cut(s)	681
EcoT14I	CCWWGG	1 cut(s)	411
ErhI	CCWWGG	1 cut(s)	411
FaeI	CATG	8 cut(s)	174, 280, 415, 428, 656, 1049, 1171, 1450
FaqI	GGGAC	1 cut(s)	558
FatI	CATG	8 cut(s)	170, 276, 411, 424, 652, 1045, 1167, 1446
FauI	CCCGC	1 cut(s)	1135
FauNDI	CATATG	1 cut(s)	517
Fnu4HI	GCNGC	2 cut(s)	657, 676
FokI	GGATG	3 cut(s)	394, 549, 665
Fsp4HI	GCNGC	2 cut(s)	657, 676
FspBI	CTAG	3 cut(s)	153, 341, 777
GlaI	GCGC	1 cut(s)	810
GluI	GCNGC	2 cut(s)	657, 676
GsuI	CTGGAG	2 cut(s)	291, 918
HaeIII	GGCC	5 cut(s)	175, 651, 675, 1079, 1440
HapII	CCGG	3 cut(s)	588, 672, 1007
HgaI	GACGC	2 cut(s)	125, 1505
HhaI	GCGC	1 cut(s)	811
Hin1II	CATG	8 cut(s)	174, 280, 415, 428, 656, 1049, 1171, 1450
Hin6I	GCGC	1 cut(s)	809
HinP1I	GCGC	1 cut(s)	809
HincII	GTYRAC	1 cut(s)	510
HindII	GTYRAC	1 cut(s)	510
HinfI	GANTC	8 cut(s)	4, 311, 435, 1020, 1178, 1187, 1324, 1426
HpaII	CCGG	3 cut(s)	588, 672, 1007
HphI	GGTGA	4 cut(s)	213, 758, 916, 1571
Hpy166II	GTNNAC	4 cut(s)	215, 510, 1197, 1245
Hpy188I	TCNGA	5 cut(s)	19, 76, 106, 115, 1296
Hpy8I	GTNNAC	4 cut(s)	215, 510, 1197, 1245
Hpy99I	CGWCG	3 cut(s)	80, 92, 397
HpyAV	CCTTC	4 cut(s)	520, 524, 737, 1441
HpyCH4III	ACNGT	4 cut(s)	856, 1194, 1252, 1586
HpyCH4IV	ACGT	1 cut(s)	1363
HpyCH4V	TGCA	6 cut(s)	563, 911, 1226, 1319, 1391, 1629
HpyF10VI	GCNNNNNNNGC	2 cut(s)	615, 1148
HpyF3I	CTNAG	9 cut(s)	177, 371, 782, 802, 872, 881, 1039, 1230, 1555
HpySE526I	ACGT	1 cut(s)	1363
Hsp92II	CATG	8 cut(s)	174, 280, 415, 428, 656, 1049, 1171, 1450
HspAI	GCGC	1 cut(s)	809
Kzo9I	GATC	3 cut(s)	868, 1460, 1633
LmnI	GCTCC	2 cut(s)	455, 1590
LweI	GCATC	6 cut(s)	687, 796, 920, 1121, 1235, 1537
MaeI	CTAG	3 cut(s)	153, 341, 777
MaeII	ACGT	1 cut(s)	1363
MaeIII	GTNAC	7 cut(s)	325, 884, 1034, 1359, 1449, 1544, 1559
MalI	GATC	3 cut(s)	870, 1462, 1635
MboI	GATC	3 cut(s)	868, 1460, 1633
MboII	GAAGA	8 cut(s)	95, 240, 273, 273, 309, 356, 362, 1426
MflI	RGATCY	1 cut(s)	868
MlsI	TGGCCA	2 cut(s)	651, 1079
MluCI	AATT	5 cut(s)	226, 244, 1091, 1122, 1576
MluNI	TGGCCA	2 cut(s)	651, 1079
MlyI	GAGTC	2 cut(s)	1014, 1333
MmeI	TCCRAC	2 cut(s)	99, 478
Mox20I	TGGCCA	2 cut(s)	651, 1079
MroXI	GAANNNNTTC	1 cut(s)	100
MscI	TGGCCA	2 cut(s)	651, 1079
MseI	TTAA	6 cut(s)	471, 1203, 1257, 1337, 1533, 1575
MslI	CAYNNNNRTG	4 cut(s)	416, 624, 804, 1073
Msp20I	TGGCCA	2 cut(s)	651, 1079
MspA1I	CMGCKG	1 cut(s)	896
MspCI	CTTAAG	1 cut(s)	1532
MspI	CCGG	3 cut(s)	588, 672, 1007
MspR9I	CCNGG	1 cut(s)	683
MvaI	CCWGG	1 cut(s)	683
MwoI	GCNNNNNNNGC	2 cut(s)	615, 1148
NcoI	CCATGG	1 cut(s)	411
NdeI	CATATG	1 cut(s)	517
NdeII	GATC	3 cut(s)	868, 1460, 1633
NheI	GCTAGC	1 cut(s)	152
NlaIII	CATG	8 cut(s)	174, 280, 415, 428, 656, 1049, 1171, 1450
NlaIV	GGNNCC	1 cut(s)	667
NmuCI	GTSAC	3 cut(s)	1359, 1449, 1559
NspI	RCATGY	1 cut(s)	1171
PagI	TCATGA	1 cut(s)	424
PcsI	WCGNNNNNNNCGW	2 cut(s)	84, 401
PdmI	GAANNNNTTC	1 cut(s)	100
PfeI	GAWTC	6 cut(s)	4, 311, 435, 1178, 1187, 1426
PinAI	ACCGGT	2 cut(s)	587, 1006
PkrI	GCNGC	2 cut(s)	658, 677
PleI	GAGTC	2 cut(s)	1014, 1332
PpsI	GAGTC	2 cut(s)	1014, 1332
Psp6I	CCWGG	1 cut(s)	681
PspGI	CCWGG	1 cut(s)	681
PspN4I	GGNNCC	1 cut(s)	667
PstNI	CAGNNNCTG	1 cut(s)	1499
PsuI	RGATCY	1 cut(s)	868
PvuII	CAGCTG	1 cut(s)	896
RsaI	GTAC	4 cut(s)	216, 605, 879, 1129
RsaNI	GTAC	4 cut(s)	215, 604, 878, 1128
RseI	CAYNNNNRTG	4 cut(s)	416, 624, 804, 1073
SaqAI	TTAA	6 cut(s)	471, 1203, 1257, 1337, 1533, 1575
SatI	GCNGC	2 cut(s)	657, 676
Sau3AI	GATC	3 cut(s)	868, 1460, 1633
SchI	GAGTC	2 cut(s)	1014, 1333
ScrFI	CCNGG	1 cut(s)	683
SfaNI	GCATC	6 cut(s)	687, 796, 920, 1121, 1235, 1537
SfcI	CTRYAG	2 cut(s)	1014, 1248
SmiMI	CAYNNNNRTG	4 cut(s)	416, 624, 804, 1073
SmlI	CTYRAG	4 cut(s)	1212, 1328, 1523, 1532
SmoI	CTYRAG	4 cut(s)	1212, 1328, 1523, 1532
Sse9I	AATT	5 cut(s)	226, 244, 1091, 1122, 1576
SsiI	CCGC	5 cut(s)	601, 657, 676, 1142, 1509
SspI	AATATT	4 cut(s)	504, 738, 1063, 1291
SspMI	CTAG	3 cut(s)	153, 341, 777
StyD4I	CCNGG	1 cut(s)	681
StyI	CCWWGG	1 cut(s)	411
TaaI	ACNGT	4 cut(s)	856, 1194, 1252, 1586
TaiI	ACGT	1 cut(s)	1366
TaqI	TCGA	7 cut(s)	36, 194, 257, 284, 395, 612, 865
TasI	AATT	5 cut(s)	226, 244, 1091, 1122, 1576
TatI	WGTACW	1 cut(s)	1127
TauI	GCSGC	2 cut(s)	659, 678
TfiI	GAWTC	6 cut(s)	4, 311, 435, 1178, 1187, 1426
Tru1I	TTAA	6 cut(s)	471, 1203, 1257, 1337, 1533, 1575
Tru9I	TTAA	6 cut(s)	471, 1203, 1257, 1337, 1533, 1575
TscAI	CASTG	3 cut(s)	294, 921, 1458
TseFI	GTSAC	3 cut(s)	1359, 1449, 1559
Tsp45I	GTSAC	3 cut(s)	1359, 1449, 1559
TspDTI	ATGAA	6 cut(s)	17, 96, 265, 363, 747, 1294
TspGWI	ACGGA	2 cut(s)	381, 679
TspRI	CASTG	3 cut(s)	294, 921, 1458
Vha464I	CTTAAG	1 cut(s)	1532
XapI	RAATTY	2 cut(s)	226, 1122
XceI	RCATGY	1 cut(s)	1171
XcmI	CCANNNNNNNNNTGG	1 cut(s)	659
XmnI	GAANNNNTTC	1 cut(s)	100
XspI	CTAG	3 cut(s)	153, 341, 777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.