Rorug01G0301500

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001966
Physical Location & Seq
Forward (+)
41739281 .. 41740165
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0301500.1

Sequence Viewer

Length: 312 bp
ATGGCGTCACCCACTCCCGGAATCCTCCTCCGGCTCCTCCAGTCCATGAACTCCGCCACTAAAGTCACCGGCGACCACCGATCTGCCCTGCTCCAAGTCATCGGCATAGTCCCCGCCCTCGCCGACTCCGACGACCTCTGGCCCAACCAAGGCTTCTATGTTCGGCTCTCCGACTCCCTCGACTCCACCTACGTCTCCCTCTCCGAGAAAGACACCGATCTCATCCTCGCCAACCGCCTCCAGCTCGGCCAGTTCGTCTACGTCGACTTTTTCTTTGATCACTTTCTCTTCGGTCAAACGGGTCGGGTATGA

Protein Analysis

103

Amino Acids

11.44

Weight (kDa)

4.74

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF936 PF06075 5 - 89 2.6e-39 Plant protein of unknown function (DUF936)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 258, 264
AciI CCGC 3 cut(s) 54, 114, 235
AcoI YGGCCR 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 17, 149
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 1 cut(s) 244
AluI AGCT 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 199
AoxI GGCC 2 cut(s) 140, 247
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 58
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 1 cut(s) 199
Bme1390I CCNGG 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 18
BpmI CTGGAG 2 cut(s) 23, 224
BpuMI CCSGG 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 148
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 149
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 2 cut(s) 40, 250
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 17, 149
BseNI ACTGG 2 cut(s) 40, 250
BseRI GAGGAG 2 cut(s) 17, 26
BshFI GGCC 2 cut(s) 142, 249
BsiSI CCGG 3 cut(s) 18, 31, 69
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 17, 149
BsmAI GTCTC 1 cut(s) 199
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 2 cut(s) 142, 249
Bsp143I GATC 3 cut(s) 80, 217, 277
BspACI CCGC 3 cut(s) 54, 114, 235
BspANI GGCC 2 cut(s) 142, 249
BspLI GGNNCC 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 2 cut(s) 40, 250
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 3 cut(s) 80, 217, 277
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 5
BstF5I GGATG 1 cut(s) 222
BstKTI GATC 3 cut(s) 83, 220, 280
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 3 cut(s) 80, 217, 277
BstSCI CCNGG 1 cut(s) 16
BsuRI GGCC 2 cut(s) 142, 249
BtsCI GGATG 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 141
CviAII CATG 1 cut(s) 46
CviJI RGCY 6 cut(s) 34, 142, 153, 166, 244, 249
CviKI_1 RGCY 6 cut(s) 34, 142, 153, 166, 244, 249
DpnI GATC 3 cut(s) 82, 219, 279
DpnII GATC 3 cut(s) 80, 217, 277
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 148
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 1 cut(s) 49
FaiI YATR 4 cut(s) 47, 107, 159, 310
FaqI GGGAC 1 cut(s) 95
FatI CATG 1 cut(s) 45
FauI CCCGC 1 cut(s) 121
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 2 cut(s) 258, 264
FokI GGATG 1 cut(s) 209
GsuI CTGGAG 2 cut(s) 23, 224
HaeIII GGCC 2 cut(s) 142, 249
HapII CCGG 3 cut(s) 18, 31, 69
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 49
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 4 cut(s) 21, 125, 173, 182
HpaII CCGG 3 cut(s) 18, 31, 69
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 2 cut(s) 259, 265
Hpy188I TCNGA 3 cut(s) 130, 172, 205
Hpy8I GTNNAC 2 cut(s) 259, 265
Hpy99I CGWCG 2 cut(s) 134, 266
HpyCH4IV ACGT 2 cut(s) 192, 261
HpySE526I ACGT 2 cut(s) 192, 261
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 80, 217, 277
LmnI GCTCC 2 cut(s) 39, 96
LpnPI CCDG 8 cut(s) 31, 44, 53, 82, 101, 124, 254, 263
MaeII ACGT 2 cut(s) 192, 261
MaeIII GTNAC 2 cut(s) 6, 64
MalI GATC 3 cut(s) 82, 219, 279
MboI GATC 3 cut(s) 80, 217, 277
MboII GAAGA 1 cut(s) 280
MlyI GAGTC 3 cut(s) 119, 167, 176
MmeI TCCRAC 2 cut(s) 153, 195
MnlI CCTC 9 cut(s) 35, 38, 47, 128, 146, 188, 209, 236, 248
MspI CCGG 3 cut(s) 18, 31, 69
MspR9I CCNGG 1 cut(s) 18
NciI CCSGG 1 cut(s) 18
NdeII GATC 3 cut(s) 80, 217, 277
NlaIII CATG 1 cut(s) 49
NlaIV GGNNCC 1 cut(s) 35
NmeAIII GCCGAG 1 cut(s) 225
NmuCI GTSAC 2 cut(s) 6, 64
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 252, 261
PfeI GAWTC 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 16
PleI GAGTC 3 cut(s) 119, 167, 176
PpsI GAGTC 3 cut(s) 119, 167, 176
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 1 cut(s) 141
SalI GTCGAC 1 cut(s) 263
Sau3AI GATC 3 cut(s) 80, 217, 277
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 3 cut(s) 119, 167, 176
ScrFI CCNGG 1 cut(s) 18
SetI ASST 5 cut(s) 138, 191, 195, 246, 264
SgrAI CRCCGGYG 1 cut(s) 68
SsiI CCGC 3 cut(s) 54, 114, 235
StyD4I CCNGG 1 cut(s) 16
StyI CCWWGG 1 cut(s) 148
TaiI ACGT 2 cut(s) 195, 264
TaqI TCGA 2 cut(s) 180, 264
TaqII GACCGA 1 cut(s) 281
TfiI GAWTC 1 cut(s) 21
TseFI GTSAC 2 cut(s) 6, 64
Tsp45I GTSAC 2 cut(s) 6, 64
TspDTI ATGAA 1 cut(s) 62
XmiI GTMKAC 2 cut(s) 258, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.