MD01G1104600.v1.1

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001966
Physical Location & Seq
Forward (+)
21786348 .. 21790046
3699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1104600.v1.1.491

Sequence Viewer

Length: 783 bp
ATGAATAACTCGGTTTCAGATCGGAGTTTTTACATTGAGAGTGAGGAGGAAGATGATGAGGAAAAGGACTTCAATAAGAGCATTGAAGACGGAGGCAACACGGATTCTTCTGCCGAATCTTTTGCTGAGAATCGGCAGCCGAATAATCCCAGTTCTTACAACACTCAATGGCCTCAAAGTTACAGGCAATCTATTGATCTGTATAGTAGTGTACCGTCCCCTGGTATTGGGTTTCTGGGCACTCCTTCATTGTCAAGACTAGGAAGTTCATATCTTTCCTCATCCCTAACAAGAAGGCACACTCCCGAAACATTGCCTTTCTTGCCGAAACCATTACCAACACTCGCAGATAAGGAACAACAACAACAGCCGCAGAGACGCAGTTCTCATTCTCTGCTTCCTCCTTTGCCAAGGAGGACTTCTGTTAGAAAAGATGAGAAAGCATCCAGAGTTACTCATGACCAGTATCCGATGTCTCGCCAGAGCTCCTTTACACAAGCTGTGTTTAATGGAATTAACGTTCTTTGCGGGGTTGGAATCCTTTCGACGCCATATGCAGTCAAGGAAGGAGGATGGCTCGGGCTCCTCGTTTTGCTTATTTTTGCAGTGCTTTCGTTCTACACCGGGTTGCTCCTGCGTTACTGCTTGGACAGCCAACCTGGACTCGAGACATACCCGGACATTGGCCAAGCTGCATTTGGTATTGGAGGCAGAATCGCCATCTCGATAGTACTGTACGTGGAATTATATTTTGGTTTGAAATGGAACACATCCATGGGATGA

Protein Analysis

261

Amino Acids

28.89

Weight (kDa)

5.47

Isoelectric Point (pI)

68.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 161 - 249 2.1e-19 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 371, 528
AclI AACGTT 1 cut(s) 519
AcoI YGGCCR 1 cut(s) 685
AcyI GRCGYC 1 cut(s) 548
AfaI GTAC 3 cut(s) 213, 732, 737
AfiI CCNNNNNNNGG 3 cut(s) 221, 227, 683
AgsI TTSAA 3 cut(s) 73, 86, 760
AjnI CCWGG 2 cut(s) 220, 658
AjuI GAANNNNNNNTTGG 4 cut(s) 403, 435, 735, 767
AloI GAACNNNNNNTCC 2 cut(s) 504, 536
AluBI AGCT 3 cut(s) 486, 500, 692
AluI AGCT 3 cut(s) 486, 500, 692
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 3 cut(s) 370, 480, 662
Ama87I CYCGRG 2 cut(s) 578, 665
AoxI GGCC 2 cut(s) 170, 685
ApeKI GCWGC 2 cut(s) 136, 692
Asp700I GAANNNNTTC 1 cut(s) 541
AsuC2I CCSGG 2 cut(s) 625, 677
AvaI CYCGRG 2 cut(s) 578, 665
BaeGI GKGCMC 1 cut(s) 242
BalI TGGCCA 1 cut(s) 687
BanII GRGCYC 2 cut(s) 488, 585
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 2 cut(s) 148, 679
BccI CCATC 2 cut(s) 567, 728
BcgI CGANNNNNNTGC 4 cut(s) 104, 138, 594, 628
BciT130I CCWGG 2 cut(s) 222, 660
BciVI GTATCC 1 cut(s) 477
BcnI CCSGG 2 cut(s) 625, 677
BcoDI GTCTC 3 cut(s) 370, 480, 662
BfaI CTAG 1 cut(s) 260
BfuI GTATCC 1 cut(s) 477
BisI GCNGC 3 cut(s) 137, 371, 693
BlsI GCNGC 3 cut(s) 138, 372, 694
BmcAI AGTACT 1 cut(s) 732
Bme1390I CCNGG 4 cut(s) 222, 625, 660, 677
BmeT110I CYCGRG 2 cut(s) 578, 665
BmiI GGNNCC 1 cut(s) 584
BmrFI CCNGG 4 cut(s) 222, 625, 660, 677
BmrI ACTGGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 561, 593
BpuMI CCSGG 2 cut(s) 625, 677
BsaAI YACGTR 1 cut(s) 739
BsaHI GRCGYC 1 cut(s) 548
BsaJI CCNNGG 3 cut(s) 220, 410, 774
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 227, 683
Bse1I ACTGG 2 cut(s) 150, 463
Bse3DI GCAATG 1 cut(s) 311
BseBI CCWGG 2 cut(s) 222, 660
BseDI CCNNGG 3 cut(s) 220, 410, 774
BseGI GGATG 4 cut(s) 281, 443, 578, 770
BseLI CCNNNNNNNGG 3 cut(s) 221, 227, 683
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 2 cut(s) 150, 463
BseRI GAGGAG 2 cut(s) 59, 575
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 2 cut(s) 148, 679
BshFI GGCC 2 cut(s) 172, 687
BsiHKAI GWGCWC 1 cut(s) 488
BsiHKCI CYCGRG 2 cut(s) 578, 665
BsiSI CCGG 2 cut(s) 624, 677
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 3 cut(s) 221, 227, 683
BsmAI GTCTC 3 cut(s) 370, 480, 662
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 2 cut(s) 172, 687
BsoBI CYCGRG 2 cut(s) 578, 665
Bsp1286I GDGCHC 3 cut(s) 242, 488, 585
Bsp143I GATC 2 cut(s) 19, 196
Bsp19I CCATGG 1 cut(s) 774
BspACI CCGC 2 cut(s) 371, 528
BspANI GGCC 2 cut(s) 172, 687
BspCNI CTCAG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 584
BsrDI GCAATG 1 cut(s) 311
BsrI ACTGG 2 cut(s) 150, 463
BssECI CCNNGG 3 cut(s) 220, 410, 774
BssMI GATC 2 cut(s) 19, 196
BssNI GRCGYC 1 cut(s) 548
BssT1I CCWWGG 2 cut(s) 410, 774
Bst2UI CCWGG 2 cut(s) 222, 660
Bst4CI ACNGT 2 cut(s) 216, 735
BstACI GRCGYC 1 cut(s) 548
BstBAI YACGTR 1 cut(s) 739
BstDEI CTNAG 1 cut(s) 126
BstDSI CCRYGG 1 cut(s) 774
BstF5I GGATG 4 cut(s) 281, 443, 578, 770
BstKTI GATC 2 cut(s) 22, 199
BstMAI GTCTC 3 cut(s) 370, 480, 662
BstMBI GATC 2 cut(s) 19, 196
BstMWI GCNNNNNNNGC 2 cut(s) 322, 651
BstNI CCWGG 2 cut(s) 222, 660
BstSCI CCNGG 4 cut(s) 220, 623, 658, 675
BstSLI GKGCMC 1 cut(s) 242
BstV1I GCAGC 2 cut(s) 148, 679
BstV2I GAAGAC 1 cut(s) 93
BsuI GTATCC 1 cut(s) 477
BsuRI GGCC 2 cut(s) 172, 687
BtgI CCRYGG 1 cut(s) 774
BtsCI GGATG 4 cut(s) 281, 443, 578, 770
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 1 cut(s) 612
CciI TCATGA 1 cut(s) 457
CseI GACGC 2 cut(s) 387, 556
Csp6I GTAC 3 cut(s) 212, 731, 736
CviAII CATG 2 cut(s) 458, 775
CviQI GTAC 3 cut(s) 212, 731, 736
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 21, 198
DpnII GATC 2 cut(s) 19, 196
EaeI YGGCCR 1 cut(s) 685
Ecl136II GAGCTC 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 410, 774
Eco24I GRGCYC 2 cut(s) 488, 585
Eco53kI GAGCTC 1 cut(s) 486
Eco88I CYCGRG 2 cut(s) 578, 665
EcoICRI GAGCTC 1 cut(s) 486
EcoRII CCWGG 2 cut(s) 220, 658
EcoT14I CCWWGG 2 cut(s) 410, 774
EcoT38I GRGCYC 2 cut(s) 488, 585
ErhI CCWWGG 2 cut(s) 410, 774
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 2 cut(s) 461, 778
FaiI YATR 8 cut(s) 204, 271, 459, 553, 555, 673, 748, 776
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 457, 774
FauI CCCGC 1 cut(s) 521
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 3 cut(s) 137, 371, 693
FokI GGATG 4 cut(s) 268, 430, 585, 757
FriOI GRGCYC 2 cut(s) 488, 585
Fsp4HI GCNGC 3 cut(s) 137, 371, 693
FspBI CTAG 1 cut(s) 260
GluI GCNGC 3 cut(s) 137, 371, 693
HaeIII GGCC 2 cut(s) 172, 687
HapII CCGG 2 cut(s) 624, 677
HgaI GACGC 2 cut(s) 387, 556
Hin1I GRCGYC 1 cut(s) 548
Hin1II CATG 2 cut(s) 461, 778
HinfI GANTC 6 cut(s) 104, 116, 130, 537, 663, 714
HpaII CCGG 2 cut(s) 624, 677
Hpy166II GTNNAC 1 cut(s) 212
Hpy188I TCNGA 3 cut(s) 19, 24, 471
Hpy188III TCNNGA 6 cut(s) 255, 305, 447, 458, 667, 724
Hpy8I GTNNAC 1 cut(s) 212
Hpy99I CGWCG 1 cut(s) 550
HpyAV CCTTC 3 cut(s) 255, 288, 560
HpyCH4III ACNGT 2 cut(s) 216, 735
HpyCH4IV ACGT 2 cut(s) 519, 738
HpyCH4V TGCA 3 cut(s) 557, 605, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 322, 651
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 2 cut(s) 519, 738
Hsp92I GRCGYC 1 cut(s) 548
Hsp92II CATG 2 cut(s) 461, 778
Kzo9I GATC 2 cut(s) 19, 196
LmnI GCTCC 3 cut(s) 491, 588, 636
Lsp1109I GCAGC 2 cut(s) 148, 679
LweI GCATC 1 cut(s) 452
MaeI CTAG 1 cut(s) 260
MaeII ACGT 2 cut(s) 519, 738
MaeIII GTNAC 3 cut(s) 179, 451, 638
MalI GATC 2 cut(s) 21, 198
MboI GATC 2 cut(s) 19, 196
MboII GAAGA 3 cut(s) 62, 98, 99
MhlI GDGCHC 3 cut(s) 242, 488, 585
MlsI TGGCCA 1 cut(s) 687
MluCI AATT 2 cut(s) 513, 743
MluNI TGGCCA 1 cut(s) 687
MlyI GAGTC 1 cut(s) 657
MmeI TCCRAC 1 cut(s) 514
Mox20I TGGCCA 1 cut(s) 687
MroXI GAANNNNTTC 1 cut(s) 541
MscI TGGCCA 1 cut(s) 687
MseI TTAA 2 cut(s) 507, 516
MslI CAYNNNNRTG 1 cut(s) 773
Msp20I TGGCCA 1 cut(s) 687
MspI CCGG 2 cut(s) 624, 677
MspR9I CCNGG 4 cut(s) 222, 625, 660, 677
MvaI CCWGG 2 cut(s) 222, 660
MwoI GCNNNNNNNGC 2 cut(s) 322, 651
NciI CCSGG 2 cut(s) 625, 677
NcoI CCATGG 1 cut(s) 774
NdeI CATATG 1 cut(s) 553
NdeII GATC 2 cut(s) 19, 196
NlaIII CATG 2 cut(s) 461, 778
NlaIV GGNNCC 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 665
PagI TCATGA 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 541
PfeI GAWTC 5 cut(s) 104, 116, 130, 537, 714
PkrI GCNGC 3 cut(s) 138, 372, 694
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
Ppu21I YACGTR 1 cut(s) 739
Psp124BI GAGCTC 1 cut(s) 488
Psp1406I AACGTT 1 cut(s) 519
Psp6I CCWGG 2 cut(s) 220, 658
PspGI CCWGG 2 cut(s) 220, 658
PspN4I GGNNCC 1 cut(s) 584
RsaI GTAC 3 cut(s) 213, 732, 737
RsaNI GTAC 3 cut(s) 212, 731, 736
RseI CAYNNNNRTG 1 cut(s) 773
SacI GAGCTC 1 cut(s) 488
SaqAI TTAA 2 cut(s) 507, 516
SatI GCNGC 3 cut(s) 137, 371, 693
Sau3AI GATC 2 cut(s) 19, 196
ScaI AGTACT 1 cut(s) 732
SchI GAGTC 1 cut(s) 657
ScrFI CCNGG 4 cut(s) 222, 625, 660, 677
SduI GDGCHC 3 cut(s) 242, 488, 585
SetI ASST 6 cut(s) 488, 502, 522, 661, 694, 741
SfaNI GCATC 1 cut(s) 452
Sfr274I CTCGAG 1 cut(s) 665
SlaI CTCGAG 1 cut(s) 665
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 2 cut(s) 513, 743
SsiI CCGC 2 cut(s) 371, 528
SspMI CTAG 1 cut(s) 260
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 4 cut(s) 220, 623, 658, 675
StyI CCWWGG 2 cut(s) 410, 774
TaaI ACNGT 2 cut(s) 216, 735
TaiI ACGT 2 cut(s) 522, 741
TaqI TCGA 3 cut(s) 545, 666, 725
TasI AATT 2 cut(s) 513, 743
TatI WGTACW 1 cut(s) 730
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 5 cut(s) 104, 116, 130, 537, 714
Tru1I TTAA 2 cut(s) 507, 516
Tru9I TTAA 2 cut(s) 507, 516
TscAI CASTG 1 cut(s) 612
TseI GCWGC 2 cut(s) 136, 692
TspDTI ATGAA 3 cut(s) 17, 237, 258
TspGWI ACGGA 2 cut(s) 105, 116
TspRI CASTG 1 cut(s) 612
XcmI CCANNNNNNNNNTGG 1 cut(s) 695
XhoI CTCGAG 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 541
XspI CTAG 1 cut(s) 260
ZrmI AGTACT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.