Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001966

Physical Location & Seq

Genomic Coordinates Forward (+)

24734061 .. 24739833

Gene Structure 5773 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1171200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGAATAATTCGGTTTCGGATCGGAGTTTTTACATTGAGAGTGAGGAAGAAGATGATGAGGAAAAGGAATTCAATAAGAGCATTGAAGACGGAGGCGAATCGGATTCTTCTGCCGAATCTTTTACTGAGAATCGGCAGCCGAATAATCCCAGTTCTCACAACAATCAATGGCCTCAAAGTTACAGGCAATCCATTGATCTCTATGGTAGTGTTCCATCCCCTAGTATTGGGTTTCTGGGCACTCCTTCATTGTCAAGACTAGGAAGTTCATTTCTTTCCTCATCCCTAACAAGAAGGTACACTCCCGAAACATTACCTTTCTTGTCAAAACCATTACCAACGGTCGCAGATGAGCAACAACACCAGCAGCAGAGGCGCAGTTCTCATTCTCTGCTTCCTCCTCTGCCAAGGAGGTCTTCGGTTAGAAAAGATGAGAAAGCATCCAGGGTTACTCATGACCATCATCCGATGCCTCGCCACAGCTCATTTGCACAAGCCGTGATCAATGGAATTAACGTTCTTTGCGGGGTTGGGATCCTTTCGACACCATACGCGGTCAAAGAAGGGGGATGGCTTGGGCTCTTCGTGTTACTTATTTTTGCAGTGCTTTCATTCTACACAGGATTGCTCCTGCGTTACTGCTTGGATAGCCAACCTGGGCTTGAGACTTACCCAGATATTGGCCAAGCTGCATTTGGTACTGGAGGCAGAATCGCCATCTCGATAATACTGTACGTGGAATTATATGCTTGTTGCATAGAATATATAATTTTGGAGAGTGATAACTTGTCTTCACTATTTCCAAATGCGCAACTAAGTTTGGGCGGATTCGTGTTAGATGCTCACCTTTTGTTTGCGATTGTGACCACCCTTGCTGTTCTTCCTACTGTATGGCTTCGAGACCTCAGCGTTCTTAGCTATATCTCAGCGGGTGGAGTCATTGCATCGATCTTGGTCGTCCTTTGCCTCCTTTGGGTTGGTCTAGTAGATGGAGTTGGCTTTGAGAGCAAAGGAACACCACTGAACCTTGCAACCCTTCCTGTTGCTATGGGTCTCTACGGTTACTGCTACTCAGGACATGCTGTTTTCCCAAACATTTATACTTCATTGGAAAACCGAAGCCAATACCCTGCAATCCTCTTAACCTGTTTTTGTATTTGCACTTTAATGTACGGTGGAGTTGCTGTCATGGGTTACACGATGTTTGGAGAATTAACACAGTCGCAGTTCACTCTCAACATGCCTCACGATTTAGTTGCGACCAAGATTGCTCTATGGACTACGGTGGTCAACCCCTTTACAAAATATCCTTTACTGCAAATTATGCAGATAATGAATGTCATCTTATGCACAAACACAAACATATATCACACAAGCGCACACAATGCATGCAATCTCGAATTTTATTTTGTCTTCGGAAAAACATCGGAAAGAACAGTAACTAGAACCACATATGCATATATTTTTTCTTTTGTTGCTCAACATCTACACACATACACACATACACACACAAAACAAAATAATAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0098588 GO:0098656 GO:0098805 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

ko04721 ko04723 ko04727 ko05032 ko05033 map04721 map04723 map04727 map05032 map05033

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

510

Amino Acids

56.44

Weight (kDa)

5.96

Isoelectric Point (pI)

56.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	160 - 493	2.3e-50	Transmembrane amino acid transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001966)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39130 AT2G39130 AT2G39130 AT3G54830 AT3G54830
fragaria_vesca	FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870 FvH4_7g18870
malus_domestica	MD01G1104600.v1.1 MD01G1104700.v1.1 MD07G1171200.v1.1
prunus_persica	Prupe.2G209900_v2.0.a1 Prupe.2G209900_v2.0.a1 Prupe.2G209900_v2.0.a1 Prupe.2G209900_v2.0.a1
pyrus_communis	pycom01g13120 pycom01g13130 pycom07g16500
rosa_chinensis	RchiOBHm_Chr1g0362811
rosa_laevigata	RLG00000027620
rosa_multiflora	Rmu_sc0012836.1_g000001
rosa_roxburghii	Rroxscaffold_4G00293750
rosa_rugosa	Rorug01G0301400 Rorug01G0301500 Rorug01G0301600 Rorug01G0301700
rosa_samantha	Rh1AG310400 Rh1BG273600 Rh1CG290800 Rh1DG304300
rosa_wichuraiana	Rw1G027520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	810
AccB7I	CCANNNNNTGG	1 cut(s)	680
AccII	CGCG	1 cut(s)	554
AciI	CCGC	4 cut(s)	525, 554, 825, 929
AclI	AACGTT	1 cut(s)	516
AclWI	GGATC	3 cut(s)	27, 529, 542
AcoI	YGGCCR	1 cut(s)	682
AcsI	RAATTY	2 cut(s)	68, 1400
AfaI	GTAC	4 cut(s)	299, 700, 734, 1172
AfiI	CCNNNNNNNGG	3 cut(s)	227, 680, 973
AgsI	TTSAA	2 cut(s)	73, 86
AjnI	CCWGG	2 cut(s)	443, 655
AjuI	GAANNNNNNNTTGG	2 cut(s)	400, 432
AloI	GAACNNNNNNTCC	2 cut(s)	501, 533
AluBI	AGCT	3 cut(s)	483, 689, 918
AluI	AGCT	3 cut(s)	483, 689, 918
Alw26I	GTCTC	3 cut(s)	659, 894, 1058
AlwI	GGATC	3 cut(s)	27, 529, 542
AoxI	GGCC	2 cut(s)	170, 682
ApeKI	GCWGC	3 cut(s)	136, 367, 689
ApoI	RAATTY	2 cut(s)	68, 1400
AspLEI	GCGC	3 cut(s)	378, 811, 1379
AsuHPI	GGTGA	1 cut(s)	836
BaeGI	GKGCMC	1 cut(s)	242
BalI	TGGCCA	1 cut(s)	684
BamHI	GGATCC	1 cut(s)	534
BanII	GRGCYC	1 cut(s)	582
BbsI	GAAGAC	4 cut(s)	93, 408, 783, 1405
BbvCI	CCTCAGC	1 cut(s)	905
BbvI	GCAGC	3 cut(s)	148, 379, 676
BccI	CCATC	5 cut(s)	223, 468, 564, 725, 983
BceAI	ACGGC	1 cut(s)	482
BcgI	CGANNNNNNTGC	2 cut(s)	1238, 1272
BciT130I	CCWGG	2 cut(s)	445, 657
BclI	TGATCA	1 cut(s)	501
BcoDI	GTCTC	3 cut(s)	659, 894, 1058
BfaI	CTAG	4 cut(s)	222, 260, 983, 1443
BisI	GCNGC	3 cut(s)	137, 368, 690
BlsI	GCNGC	3 cut(s)	138, 369, 691
Bme1390I	CCNGG	2 cut(s)	445, 657
BmiI	GGNNCC	1 cut(s)	536
BmrFI	CCNGG	2 cut(s)	445, 657
BmrI	ACTGGG	1 cut(s)	144
BmsI	GCATC	4 cut(s)	449, 459, 829, 953
BmuI	ACTGGG	1 cut(s)	144
BpiI	GAAGAC	4 cut(s)	93, 408, 783, 1405
BpmI	CTGGAG	1 cut(s)	723
Bpu10I	CCTNAGC	1 cut(s)	905
BpuEI	CTTGAG	1 cut(s)	683
Bsa29I	ATCGAT	1 cut(s)	947
BsaAI	YACGTR	1 cut(s)	736
BsaI	GGTCTC	2 cut(s)	894, 1058
BsaJI	CCNNGG	3 cut(s)	407, 444, 656
BsaXI	ACNNNNNCTCC	2 cut(s)	1170, 1200
Bsc4I	CCNNNNNNNGG	3 cut(s)	227, 680, 973
Bse1I	ACTGG	2 cut(s)	150, 706
Bse3DI	GCAATG	1 cut(s)	939
BseBI	CCWGG	2 cut(s)	445, 657
BseCI	ATCGAT	1 cut(s)	947
BseDI	CCNNGG	3 cut(s)	407, 444, 656
BseGI	GGATG	5 cut(s)	215, 281, 440, 463, 575
BseLI	CCNNNNNNNGG	3 cut(s)	227, 680, 973
BseMI	GCAATG	1 cut(s)	939
BseMII	CTCAG	4 cut(s)	117, 919, 939, 1086
BseNI	ACTGG	2 cut(s)	150, 706
BseRI	GAGGAG	1 cut(s)	390
BseSI	GKGCMC	1 cut(s)	242
BseXI	GCAGC	3 cut(s)	148, 379, 676
Bsh1236I	CGCG	1 cut(s)	554
Bsh1285I	CGRYCG	1 cut(s)	345
BshFI	GGCC	2 cut(s)	172, 684
BshVI	ATCGAT	1 cut(s)	947
BsiEI	CGRYCG	1 cut(s)	345
BslI	CCNNNNNNNGG	3 cut(s)	227, 680, 973
BsmAI	GTCTC	3 cut(s)	659, 894, 1058
BsnI	GGCC	2 cut(s)	172, 684
Bso31I	GGTCTC	2 cut(s)	894, 1058
Bsp1286I	GDGCHC	2 cut(s)	242, 582
Bsp143I	GATC	5 cut(s)	19, 196, 501, 534, 948
BspACI	CCGC	4 cut(s)	525, 554, 825, 929
BspANI	GGCC	2 cut(s)	172, 684
BspCNI	CTCAG	4 cut(s)	118, 918, 938, 1085
BspDI	ATCGAT	1 cut(s)	947
BspFNI	CGCG	1 cut(s)	554
BspHI	TCATGA	1 cut(s)	454
BspLI	GGNNCC	1 cut(s)	536
BspPI	GGATC	3 cut(s)	27, 529, 542
BspQI	GCTCTTC	1 cut(s)	587
BspTNI	GGTCTC	2 cut(s)	894, 1058
BsrDI	GCAATG	1 cut(s)	939
BsrI	ACTGG	2 cut(s)	150, 706
BssECI	CCNNGG	3 cut(s)	407, 444, 656
BssMI	GATC	5 cut(s)	19, 196, 501, 534, 948
BssT1I	CCWWGG	1 cut(s)	407
Bst2UI	CCWGG	2 cut(s)	445, 657
Bst4CI	ACNGT	8 cut(s)	343, 732, 889, 1061, 1175, 1221, 1285, 1438
Bst6I	CTCTTC	1 cut(s)	587
BstAPI	GCANNNNNTGC	2 cut(s)	1324, 1385
BstBAI	YACGTR	1 cut(s)	736
BstC8I	GCNNGC	1 cut(s)	1390
BstDEI	CTNAG	6 cut(s)	126, 815, 905, 914, 925, 1072
BstF5I	GGATG	5 cut(s)	215, 281, 440, 463, 575
BstFNI	CGCG	1 cut(s)	554
BstHHI	GCGC	3 cut(s)	378, 811, 1379
BstKTI	GATC	5 cut(s)	22, 199, 504, 537, 951
BstMAI	GTCTC	3 cut(s)	659, 894, 1058
BstMBI	GATC	5 cut(s)	19, 196, 501, 534, 948
BstMCI	CGRYCG	1 cut(s)	345
BstMWI	GCNNNNNNNGC	6 cut(s)	373, 648, 915, 1005, 1324, 1385
BstNI	CCWGG	2 cut(s)	445, 657
BstNSI	RCATGY	3 cut(s)	1082, 1243, 1392
BstSCI	CCNGG	2 cut(s)	443, 655
BstSLI	GKGCMC	1 cut(s)	242
BstUI	CGCG	1 cut(s)	554
BstV1I	GCAGC	3 cut(s)	148, 379, 676
BstV2I	GAAGAC	4 cut(s)	93, 408, 783, 1405
BstX2I	RGATCY	1 cut(s)	534
BstYI	RGATCY	1 cut(s)	534
Bsu15I	ATCGAT	1 cut(s)	947
BsuRI	GGCC	2 cut(s)	172, 684
BsuTUI	ATCGAT	1 cut(s)	947
BtsCI	GGATG	5 cut(s)	215, 281, 440, 463, 575
BtsI	GCAGTG	1 cut(s)	609
BtsIMutI	CAGTG	2 cut(s)	609, 1019
Cac8I	GCNNGC	1 cut(s)	1390
CciI	TCATGA	1 cut(s)	454
CfoI	GCGC	3 cut(s)	378, 811, 1379
ClaI	ATCGAT	1 cut(s)	947
Csp6I	GTAC	4 cut(s)	298, 699, 733, 1171
CviAII	CATG	5 cut(s)	455, 1079, 1189, 1240, 1389
CviQI	GTAC	4 cut(s)	298, 699, 733, 1171
DdeI	CTNAG	6 cut(s)	126, 815, 905, 914, 925, 1072
DpnI	GATC	5 cut(s)	21, 198, 503, 536, 950
DpnII	GATC	5 cut(s)	19, 196, 501, 534, 948
EaeI	YGGCCR	1 cut(s)	682
Eam1104I	CTCTTC	1 cut(s)	587
EarI	CTCTTC	1 cut(s)	587
EciI	GGCGGA	1 cut(s)	840
Eco130I	CCWWGG	1 cut(s)	407
Eco24I	GRGCYC	1 cut(s)	582
Eco31I	GGTCTC	2 cut(s)	894, 1058
EcoRI	GAATTC	1 cut(s)	68
EcoRII	CCWGG	2 cut(s)	443, 655
EcoT14I	CCWWGG	1 cut(s)	407
EcoT22I	ATGCAT	2 cut(s)	1390, 1459
EcoT38I	GRGCYC	1 cut(s)	582
ErhI	CCWWGG	1 cut(s)	407
FaeI	CATG	5 cut(s)	458, 1082, 1192, 1243, 1392
FalI	AAGNNNNNCTT	2 cut(s)	400, 432
FatI	CATG	5 cut(s)	454, 1078, 1188, 1239, 1388
FauI	CCCGC	2 cut(s)	518, 922
FauNDI	CATATG	1 cut(s)	1453
FbaI	TGATCA	1 cut(s)	501
Fnu4HI	GCNGC	3 cut(s)	137, 368, 690
FokI	GGATG	5 cut(s)	202, 268, 427, 450, 582
FriOI	GRGCYC	1 cut(s)	582
Fsp4HI	GCNGC	3 cut(s)	137, 368, 690
FspBI	CTAG	4 cut(s)	222, 260, 983, 1443
FspI	TGCGCA	1 cut(s)	810
GlaI	GCGC	3 cut(s)	377, 810, 1378
GluI	GCNGC	3 cut(s)	137, 368, 690
GsuI	CTGGAG	1 cut(s)	723
HaeIII	GGCC	2 cut(s)	172, 684
HhaI	GCGC	3 cut(s)	378, 811, 1379
Hin1II	CATG	5 cut(s)	458, 1082, 1192, 1243, 1392
Hin6I	GCGC	3 cut(s)	376, 809, 1377
HinP1I	GCGC	3 cut(s)	376, 809, 1377
HincII	GTYRAC	1 cut(s)	1291
HindII	GTYRAC	1 cut(s)	1291
HinfI	GANTC	7 cut(s)	98, 104, 116, 130, 711, 828, 936
HphI	GGTGA	1 cut(s)	836
Hpy166II	GTNNAC	3 cut(s)	300, 1230, 1291
Hpy188I	TCNGA	6 cut(s)	19, 24, 103, 468, 1418, 1429
Hpy188III	TCNNGA	8 cut(s)	255, 305, 455, 721, 899, 1074, 1247, 1397
Hpy8I	GTNNAC	3 cut(s)	300, 1230, 1291
HpyAV	CCTTC	4 cut(s)	255, 288, 557, 1046
HpyCH4III	ACNGT	8 cut(s)	343, 732, 889, 1061, 1175, 1221, 1285, 1438
HpyCH4IV	ACGT	2 cut(s)	516, 735
HpyF10VI	GCNNNNNNNGC	6 cut(s)	373, 648, 915, 1005, 1324, 1385
HpyF3I	CTNAG	6 cut(s)	126, 815, 905, 914, 925, 1072
HpySE526I	ACGT	2 cut(s)	516, 735
Hsp92II	CATG	5 cut(s)	458, 1082, 1192, 1243, 1392
HspAI	GCGC	3 cut(s)	376, 809, 1377
Ksp22I	TGATCA	1 cut(s)	501
Kzo9I	GATC	5 cut(s)	19, 196, 501, 534, 948
LguI	GCTCTTC	1 cut(s)	587
LmnI	GCTCC	1 cut(s)	633
Lsp1109I	GCAGC	3 cut(s)	148, 379, 676
LweI	GCATC	4 cut(s)	449, 459, 829, 953
MaeI	CTAG	4 cut(s)	222, 260, 983, 1443
MaeII	ACGT	2 cut(s)	516, 735
MaeIII	GTNAC	8 cut(s)	179, 448, 588, 635, 862, 1061, 1193, 1438
MalI	GATC	5 cut(s)	21, 198, 503, 536, 950
MboI	GATC	5 cut(s)	19, 196, 501, 534, 948
MboII	GAAGA	9 cut(s)	59, 62, 98, 99, 408, 574, 783, 872, 1405
MflI	RGATCY	1 cut(s)	534
MhlI	GDGCHC	2 cut(s)	242, 582
MlsI	TGGCCA	1 cut(s)	684
MluCI	AATT	8 cut(s)	7, 68, 510, 740, 768, 1211, 1320, 1400
MluNI	TGGCCA	1 cut(s)	684
MlyI	GAGTC	1 cut(s)	945
Mox20I	TGGCCA	1 cut(s)	684
Mph1103I	ATGCAT	2 cut(s)	1390, 1459
MscI	TGGCCA	1 cut(s)	684
MseI	TTAA	4 cut(s)	513, 1142, 1166, 1214
MslI	CAYNNNNRTG	1 cut(s)	1166
Msp20I	TGGCCA	1 cut(s)	684
MspA1I	CMGCKG	1 cut(s)	929
MspR9I	CCNGG	2 cut(s)	445, 657
MvaI	CCWGG	2 cut(s)	445, 657
MvnI	CGCG	1 cut(s)	554
MwoI	GCNNNNNNNGC	6 cut(s)	373, 648, 915, 1005, 1324, 1385
NdeI	CATATG	1 cut(s)	1453
NdeII	GATC	5 cut(s)	19, 196, 501, 534, 948
NlaIII	CATG	5 cut(s)	458, 1082, 1192, 1243, 1392
NlaIV	GGNNCC	1 cut(s)	536
NmuCI	GTSAC	1 cut(s)	862
NsbI	TGCGCA	1 cut(s)	810
NsiI	ATGCAT	2 cut(s)	1390, 1459
NspI	RCATGY	3 cut(s)	1082, 1243, 1392
PaeI	GCATGC	1 cut(s)	1392
PagI	TCATGA	1 cut(s)	454
PciSI	GCTCTTC	1 cut(s)	587
PfeI	GAWTC	6 cut(s)	98, 104, 116, 130, 711, 828
PflMI	CCANNNNNTGG	1 cut(s)	680
PkrI	GCNGC	3 cut(s)	138, 369, 691
PleI	GAGTC	1 cut(s)	944
PpsI	GAGTC	1 cut(s)	944
Ppu21I	YACGTR	1 cut(s)	736
Psp1406I	AACGTT	1 cut(s)	516
Psp6I	CCWGG	2 cut(s)	443, 655
PspGI	CCWGG	2 cut(s)	443, 655
PspN4I	GGNNCC	1 cut(s)	536
PsuI	RGATCY	1 cut(s)	534
RsaI	GTAC	4 cut(s)	299, 700, 734, 1172
RsaNI	GTAC	4 cut(s)	298, 699, 733, 1171
RseI	CAYNNNNRTG	1 cut(s)	1166
SapI	GCTCTTC	1 cut(s)	587
SaqAI	TTAA	4 cut(s)	513, 1142, 1166, 1214
SatI	GCNGC	3 cut(s)	137, 368, 690
Sau3AI	GATC	5 cut(s)	19, 196, 501, 534, 948
SchI	GAGTC	1 cut(s)	945
ScrFI	CCNGG	2 cut(s)	445, 657
SduI	GDGCHC	2 cut(s)	242, 582
SfaNI	GCATC	4 cut(s)	449, 459, 829, 953
SmiMI	CAYNNNNRTG	1 cut(s)	1166
SmlI	CTYRAG	1 cut(s)	662
SmoI	CTYRAG	1 cut(s)	662
SphI	GCATGC	1 cut(s)	1392
Sse9I	AATT	8 cut(s)	7, 68, 510, 740, 768, 1211, 1320, 1400
SsiI	CCGC	4 cut(s)	525, 554, 825, 929
SspMI	CTAG	4 cut(s)	222, 260, 983, 1443
StyD4I	CCNGG	2 cut(s)	443, 655
StyI	CCWWGG	1 cut(s)	407
TaaI	ACNGT	8 cut(s)	343, 732, 889, 1061, 1175, 1221, 1285, 1438
TaiI	ACGT	2 cut(s)	519, 738
TaqI	TCGA	5 cut(s)	542, 722, 898, 947, 1398
TasI	AATT	8 cut(s)	7, 68, 510, 740, 768, 1211, 1320, 1400
TfiI	GAWTC	6 cut(s)	98, 104, 116, 130, 711, 828
Tru1I	TTAA	4 cut(s)	513, 1142, 1166, 1214
Tru9I	TTAA	4 cut(s)	513, 1142, 1166, 1214
TscAI	CASTG	2 cut(s)	609, 1026
TseFI	GTSAC	1 cut(s)	862
TseI	GCWGC	3 cut(s)	136, 367, 689
Tsp45I	GTSAC	1 cut(s)	862
TspDTI	ATGAA	6 cut(s)	17, 237, 258, 600, 1095, 1349
TspGWI	ACGGA	1 cut(s)	105
TspRI	CASTG	2 cut(s)	609, 1026
Van91I	CCANNNNNTGG	1 cut(s)	680
XapI	RAATTY	2 cut(s)	68, 1400
XceI	RCATGY	3 cut(s)	1082, 1243, 1392
XcmI	CCANNNNNNNNNTGG	1 cut(s)	692
XspI	CTAG	4 cut(s)	222, 260, 983, 1443
Zsp2I	ATGCAT	2 cut(s)	1390, 1459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.