AT3G57330

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002172
Physical Location & Seq
Reverse (-)
21211014 .. 21216836
5823 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G57330.2

Sequence Viewer

Length: 3078 bp
ATGTCTAATCTACTAAAGGATTTCGAGGTCGCATCTAAGAATCCGTCGCTGGAAGCTCGTCAACGATGGCGATCCTCCGTGGGACTCGTAAAAAATCGGGCTCGTCGTTTCCGTATGATCAGTAATCTCGACAAACTCGCTGAGAATGAGAAGAAGAGATGCCAGATCCAGGAAAAGATAAGGGTTGTCTTCTATGTTCAAAAGGCAGCTTTTCAGTTTATTGATGCTGGTGCCCGCCCTGAATATAAACTCACTGATGAGGTCAAAAAAGCAGGATTTTATGTCGAAGCAGATGAGCTTGCTTCTATGGTTCGCAATCATGACACAAAGAGCCTTACAAAAATCGGAGGACCTGAAGGAATCGCTCAGAAAGTATCTGTATCTCTCGCTGAAGGTGTACGTTCGAGTGAACTACACATCAGAGAAAAAATCTATGGAGAAAACCGTTATACTGAGAAACCAGCCAGATCATTTTTAACGTTTGTTTGGGAAGCGCTTCAAGACATTACCCTTATCATCCTTATGGTCTGCGCTGTAGTATCAATAGGAGTGGGAGTTGCAACAGAAGGTTTTCCAAAGGGCATGTATGATGGGACGGGGATCTTGCTTAGTATAATCTTGGTGGTCATGGTTACTGCTATCAGTGACTACAAGCAATCTTTGCAGTTCAGAGATTTGGATCGAGAGAAAAAGAAGATTATTATTCAAGTCACCAGGGATGGCAGCAGACAAGAGGTCTCCATCCATGACTTGGTTGTTGGAGATGTGGTCCATTTATCTATTGGGGATCAAGTTCCAGCTGATGGGATTTTTATATCCGGATACAATCTGGAGATAGACGAGTCAAGCTTATCGGGCGAGAGTGAACCATCACATGTGAATAAAGAGAAGCCGTTTCTGCTTTCAGGAACCAAAGTCCAAAACGGCTCTGCAAAAATGCTGGTGACAACCGTTGGTATGAGGACTGAGTGGGGAAAGTTGATGGATACTCTGAGTGAGGGTGGAGAGGATGAGACCCCTCTGCAGGTGAAACTAAATGGTGTTGCAACAATCATAGGTAAGATTGGTTTAGGCTTTGCAGTGCTTACGTTTGTGGTGTTGTGCATAAGGTTCGTTGTGGAGAAAGCCACGGCTGGTAGCATCACTGAGTGGTCTTCTGAAGATGCACTAACATTGCTTGACTACTTTGCAATTGCTGTTACCATTATAGTCGTTGCTGTACCTGAAGGGTTACCTTTGGCTGTGACCTTAAGTCTAGCATTTGCTATGAAGCAATTAATGAGTGATAGGGCACTTGTAAGGCATCTGGCTGCATGTGAGACGATGGGTTCTTCTACCTGCATCTGCACGGATAAAACAGGGACCTTAACCACTAACCACATGGTAGTCAACAAAGTTTGGATATGTGAAAATATCAAAGAGAGGCAAGAGGAAAACTTCCAGTTGAACCTATCCGAACAAGTTAAGAATATCCTTATACAGGCCATATTTCAGAACACTGGTTCTGAAGTTGTTAAGGATAAGGAAGGAAAAACTCAGATCCTGGGTTCACCTACAGAAAGGGCGATACTGGAATTTGGTTTGCTTTTGGGTGGTGACGTTGACACGCAACGCCGAGAACATAAGATACTCAAGATCGAGCCATTCAACTCAGACAAGAAGAAAATGTCTGTTCTTACATCTCATTCAGGTGGTAAAGTACGTGCTTTCTGCAAAGGTGCATCTGAAATAGTCTTGAAAATGTGTGAGAAGGTTGTGGATTCAAACGGAGAATCTGTTCCTTTATCTGAAGAAAAGATTGCAAGTATCTCTGATGTCATAGAGGGATTTGCTTCAGAGGCTCTTAGGACTCTCTGCTTAGTTTACACGGATCTTGATGAAGCTCCCAGAGGGGATCTTCCTAATGGTGGCTACACATTGGTAGCAGTTGTTGGAATCAAGGACCCGGTTCGGCCTGGTGTTAGGGAAGCCGTTCAGACTTGTCAAGCTGCTGGAATCACTGTTCGTATGGTCACTGGTGATAACATTAGCACTGCCAAAGCGATTGCAAAGGAATGTGGTATACTTACAGCAGGAGGTGTAGCTATAGAAGGTTCAGACTTCCGTAATTTGCCTCCACATGAAATGAGGGCCATTCTACCGAAAATCCAGGTGATGGCTCGGTCTTTGCCATTGGACAAACATACATTAGTCAACAACTTGAGGAAAATGGGAGAGGTAGTTGCAGTGACTGGGGATGGAACAAATGATGCTCCTGCATTGCATGAGGCTGACATTGGACTTGCCATGGGAATAGCTGGAACAGAGGTTGCGAAAGAGAATGCTGATGTTATCATAATGGACGACAACTTTGCAACAATAGTGAATGTGGCAAAATGGGGACGTGCTGTGTATATCAACATCCAGAAGTTTGTTCAGTTCCAGCTAACTGTTAATGTTGTTGCTCTGATCATCAACTTTGTCTCTGCTTGCATCACTGGATCTGCGCCACTCACCGCAGTGCAACTGCTTTGGGTGAACATGATCATGGACACACTTGGTGCATTGGCTCTAGCGACCGAACCTCCCAACGAAGGTTTAATGAAACGCCAACCAATCGGTAGAACAGCAAGCTTCATCACCAGAGCTATGTGGAGAAACATCATTGGTCAAAGCATTTATCAGTTAATCGTTCTCGGCATCCTCAACTTTGCTGGCAAACAAATACTCAACCTTAATGGCCCAGATTCAACAATTGTTCTCAACACAATTATCTTCAACTCTTTCGTCTTTTGCCAGGTGTTTAATGAAGTAAACAGCAGAGAGATAGAGAAGATAAATGTGTTCGAAGGAATGTTCAAGAGTTGGGTGTTTGTTGCTGTAATGACTGCAACAGTTGGATTCCAAGTGATCATTGTTGAGTTCCTTGGAGCATTTGCAAGCACTGTTCCTTTGAGCTGGCAACATTGGCTACTCTGCATTTTGATTGGTTCCGTAAGCATGATTCTAGCCGTTGGTCTTAAATGCATACCGGTTGAGTCTAACCGTCATCACGATGGCTATGAGCTGCTTCCTTCTGGTCCATCTGACTCTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1025

Amino Acids

111.94

Weight (kDa)

5.97

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 61 - 126 1.6e-12 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 180 - 275 6e-23 P-type ATPase actuator domain
Cation_ATPase PF13246 437 - 597 4.6e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 580 - 703 3.2e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 774 - 944 2.7e-43 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1015
Acc36I ACCTGC 2 cut(s) 1015, 1346
AccB1I GGYRCC 1 cut(s) 230
AccB7I CCANNNNNTGG 3 cut(s) 751, 803, 2155
AccI GTMKAC 1 cut(s) 2062
AccIII TCCGGA 1 cut(s) 818
AciI CCGC 2 cut(s) 235, 2496
AclI AACGTT 1 cut(s) 479
AclWI GGATC 9 cut(s) 66, 160, 608, 687, 795, 1534, 1878, 1902, 2488
AcsI RAATTY 1 cut(s) 1574
AcuI CTGAAG 7 cut(s) 375, 411, 1179, 1245, 1527, 1809, 1818
AdeI CACNNNGTG 2 cut(s) 1149, 2540
AfaI GTAC 3 cut(s) 399, 1221, 1701
AfeI AGCGCT 1 cut(s) 495
AfiI CCNNNNNNNGG 8 cut(s) 169, 751, 803, 1024, 1480, 1907, 2155, 2573
AflII CTTAAG 1 cut(s) 1249
AflIII ACRYGT 1 cut(s) 874
AgeI ACCGGT 1 cut(s) 3010
AhdI GACNNNNNGTC 2 cut(s) 734, 1251
AjiI CACGTC 1 cut(s) 2384
AjnI CCWGG 6 cut(s) 168, 713, 1542, 1954, 2148, 2775
AleI CACNNNNGTG 1 cut(s) 2498
AloI GAACNNNNNNTCC 4 cut(s) 1761, 1793, 1986, 2018
Alw26I GTCTC 4 cut(s) 742, 1007, 1313, 2467
AlwI GGATC 9 cut(s) 66, 160, 608, 687, 795, 1534, 1878, 1902, 2488
AlwNI CAGNNNCTG 2 cut(s) 1543, 2231
Aor13HI TCCGGA 1 cut(s) 818
Aor51HI AGCGCT 1 cut(s) 495
AoxI GGCC 4 cut(s) 1482, 1952, 2130, 2719
ApeKI GCWGC 5 cut(s) 206, 723, 1310, 1988, 3046
ApoI RAATTY 1 cut(s) 1574
ArsI GACNNNNNNTTYG 2 cut(s) 2333, 2365
AseI ATTAAT 1 cut(s) 1277
AsiGI ACCGGT 1 cut(s) 3010
Asp700I GAANNNNTTC 4 cut(s) 495, 570, 1776, 1971
AspLEI GCGC 3 cut(s) 496, 533, 2488
AspS9I GGNCC 7 cut(s) 350, 769, 1362, 1942, 2130, 2720, 3059
AsuC2I CCSGG 1 cut(s) 1946
AsuII TTCGAA 1 cut(s) 2826
AvaII GGWCC 5 cut(s) 350, 769, 1362, 1942, 3059
BaeGI GKGCMC 2 cut(s) 235, 1294
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 103
BbsI GAAGAC 2 cut(s) 181, 1146
BbvI GCAGC 5 cut(s) 218, 735, 1297, 1975, 3033
BceAI ACGGC 5 cut(s) 877, 940, 1146, 1955, 2975
BcgI CGANNNNNNTGC 4 cut(s) 1093, 1127, 2333, 2367
BciT130I CCWGG 6 cut(s) 170, 715, 1544, 1956, 2150, 2777
BciVI GTATCC 2 cut(s) 815, 979
BclI TGATCA 4 cut(s) 117, 2448, 2523, 2889
BcnI CCSGG 1 cut(s) 1946
BcoDI GTCTC 4 cut(s) 742, 1007, 1313, 2467
BfaI CTAG 3 cut(s) 1256, 2552, 2987
BfmI CTRYAG 4 cut(s) 534, 1022, 1554, 2085
BfoI RGCGCY 1 cut(s) 497
BfrI CTTAAG 1 cut(s) 1249
BfuAI ACCTGC 2 cut(s) 1015, 1346
BfuI GTATCC 2 cut(s) 815, 979
BisI GCNGC 5 cut(s) 207, 724, 1311, 1989, 3047
BlsI GCNGC 5 cut(s) 208, 725, 1312, 1990, 3048
Bme1390I CCNGG 7 cut(s) 170, 715, 1544, 1946, 1956, 2150, 2777
Bme18I GGWCC 5 cut(s) 350, 769, 1362, 1942, 3059
BmeRI GACNNNNNGTC 2 cut(s) 734, 1251
BmgBI CACGTC 1 cut(s) 2384
BmgT120I GGNCC 7 cut(s) 350, 769, 1362, 1942, 2130, 2720, 3059
BmiI GGNNCC 5 cut(s) 232, 910, 1363, 1944, 2971
BmrFI CCNGG 7 cut(s) 170, 715, 1544, 1946, 1956, 2150, 2777
BmrI ACTGGG 1 cut(s) 2241
BmuI ACTGGG 1 cut(s) 2241
BpiI GAAGAC 2 cut(s) 181, 1146
BpmI CTGGAG 1 cut(s) 851
Bpu14I TTCGAA 1 cut(s) 2826
BpuEI CTTGAG 2 cut(s) 1616, 2221
BpuMI CCSGG 1 cut(s) 1946
BsaAI YACGTR 1 cut(s) 1703
BsaBI GATNNNNATC 2 cut(s) 70, 678
BsaI GGTCTC 2 cut(s) 742, 1007
BsaJI CCNNGG 6 cut(s) 78, 714, 1128, 1543, 2286, 2905
BsaWI WCCGGW 2 cut(s) 818, 3010
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 751, 803, 1024, 1480, 1907, 2155, 2573
Bse118I RCCGGY 1 cut(s) 3010
Bse1I ACTGG 6 cut(s) 1441, 1504, 1575, 2020, 2236, 2482
Bse3DI GCAATG 2 cut(s) 1172, 2258
Bse8I GATNNNNATC 2 cut(s) 70, 678
BseAI TCCGGA 1 cut(s) 818
BseBI CCWGG 6 cut(s) 170, 715, 1544, 1956, 2150, 2777
BseDI CCNNGG 6 cut(s) 78, 714, 1128, 1543, 2286, 2905
BseGI GGATG 7 cut(s) 516, 724, 741, 1015, 2242, 2400, 2679
BseJI GATNNNNATC 2 cut(s) 70, 678
BseLI CCNNNNNNNGG 8 cut(s) 169, 751, 803, 1024, 1480, 1907, 2155, 2573
BseMI GCAATG 2 cut(s) 1172, 2258
BseMII CTCAG 8 cut(s) 132, 380, 444, 957, 983, 1137, 1550, 1665
BseNI ACTGG 6 cut(s) 1441, 1504, 1575, 2020, 2236, 2482
BseSI GKGCMC 2 cut(s) 235, 1294
BseXI GCAGC 5 cut(s) 218, 735, 1297, 1975, 3033
BsgI GTGCAG 1 cut(s) 1330
Bsh1285I CGRYCG 1 cut(s) 2559
BshFI GGCC 4 cut(s) 1484, 1954, 2132, 2721
BshNI GGYRCC 1 cut(s) 230
BshTI ACCGGT 1 cut(s) 3010
BsiEI CGRYCG 1 cut(s) 2559
BsiSI CCGG 3 cut(s) 819, 1946, 3011
BslFI GGGAC 4 cut(s) 96, 607, 1375, 2394
BslI CCNNNNNNNGG 8 cut(s) 169, 751, 803, 1024, 1480, 1907, 2155, 2573
BsmAI GTCTC 4 cut(s) 742, 1007, 1313, 2467
BsmBI CGTCTC 1 cut(s) 1313
BsmFI GGGAC 4 cut(s) 96, 607, 1375, 2394
BsmI GAATGC 1 cut(s) 2326
BsnI GGCC 4 cut(s) 1484, 1954, 2132, 2721
Bso31I GGTCTC 2 cut(s) 742, 1007
Bsp119I TTCGAA 1 cut(s) 2826
Bsp1286I GDGCHC 3 cut(s) 103, 235, 1294
Bsp13I TCCGGA 1 cut(s) 818
Bsp19I CCATGG 1 cut(s) 2286
BspACI CCGC 2 cut(s) 235, 2496
BspANI GGCC 4 cut(s) 1484, 1954, 2132, 2721
BspCNI CTCAG 8 cut(s) 133, 379, 445, 958, 984, 1138, 1549, 1664
BspEI TCCGGA 1 cut(s) 818
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 5 cut(s) 232, 910, 1363, 1944, 2971
BspMAI CTGCAG 1 cut(s) 1026
BspMI ACCTGC 2 cut(s) 1015, 1346
BspPI GGATC 9 cut(s) 66, 160, 608, 687, 795, 1534, 1878, 1902, 2488
BspT104I TTCGAA 1 cut(s) 2826
BspT107I GGYRCC 1 cut(s) 230
BspTI CTTAAG 1 cut(s) 1249
BspTNI GGTCTC 2 cut(s) 742, 1007
BsrDI GCAATG 2 cut(s) 1172, 2258
BsrFI RCCGGY 1 cut(s) 3010
BsrI ACTGG 6 cut(s) 1441, 1504, 1575, 2020, 2236, 2482
BssAI RCCGGY 1 cut(s) 3010
BssECI CCNNGG 6 cut(s) 78, 714, 1128, 1543, 2286, 2905
BssNAI GTATAC 1 cut(s) 2063
BssT1I CCWWGG 2 cut(s) 2286, 2905
Bst1107I GTATAC 1 cut(s) 2063
Bst2UI CCWGG 6 cut(s) 170, 715, 1544, 1956, 2150, 2777
Bst4CI ACNGT 7 cut(s) 446, 952, 2002, 2431, 2875, 2926, 3026
Bst6I CTCTTC 1 cut(s) 149
BstAFI CTTAAG 1 cut(s) 1249
BstAPI GCANNNNNTGC 1 cut(s) 661
BstBAI YACGTR 1 cut(s) 1703
BstBI TTCGAA 1 cut(s) 2826
BstC8I GCNNGC 7 cut(s) 235, 300, 2470, 2611, 2695, 2920, 2939
BstDSI CCRYGG 3 cut(s) 78, 1128, 2286
BstEII GGTNACC 1 cut(s) 1230
BstENI CCTNNNNNAGG 1 cut(s) 1478
BstF5I GGATG 7 cut(s) 516, 724, 741, 1015, 2242, 2400, 2679
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 3 cut(s) 496, 533, 2488
BstMAI GTCTC 4 cut(s) 742, 1007, 1313, 2467
BstMCI CGRYCG 1 cut(s) 2559
BstMWI GCNNNNNNNGC 5 cut(s) 661, 855, 898, 1838, 2947
BstNI CCWGG 6 cut(s) 170, 715, 1544, 1956, 2150, 2777
BstNSI RCATGY 3 cut(s) 586, 878, 1317
BstPI GGTNACC 1 cut(s) 1230
BstSCI CCNGG 7 cut(s) 168, 713, 1542, 1944, 1954, 2148, 2775
BstSFI CTRYAG 4 cut(s) 534, 1022, 1554, 2085
BstSLI GKGCMC 2 cut(s) 235, 1294
BstV1I GCAGC 5 cut(s) 218, 735, 1297, 1975, 3033
BstV2I GAAGAC 2 cut(s) 181, 1146
BstX2I RGATCY 6 cut(s) 165, 600, 1539, 1870, 1894, 2480
BstYI RGATCY 6 cut(s) 165, 600, 1539, 1870, 1894, 2480
BstZ17I GTATAC 1 cut(s) 2063
BsuI GTATCC 2 cut(s) 815, 979
BsuRI GGCC 4 cut(s) 1484, 1954, 2132, 2721
BtgI CCRYGG 3 cut(s) 78, 1128, 2286
BtrI CACGTC 1 cut(s) 2384
BtsCI GGATG 7 cut(s) 516, 724, 741, 1015, 2242, 2400, 2679
BtsI GCAGTG 4 cut(s) 1086, 2031, 2232, 2505
BveI ACCTGC 2 cut(s) 1015, 1346
Cac8I GCNNGC 7 cut(s) 235, 300, 2470, 2611, 2695, 2920, 2939
CaiI CAGNNNCTG 2 cut(s) 1543, 2231
CciI TCATGA 1 cut(s) 319
CfoI GCGC 3 cut(s) 496, 533, 2488
Cfr10I RCCGGY 1 cut(s) 3010
Cfr13I GGNCC 7 cut(s) 350, 769, 1362, 1942, 2130, 2720, 3059
Csp6I GTAC 3 cut(s) 398, 1220, 1700
CspAI ACCGGT 1 cut(s) 3010
CspCI CAANNNNNGTGG 2 cut(s) 531, 566
CviQI GTAC 3 cut(s) 398, 1220, 1700
DraIII CACNNNGTG 2 cut(s) 1149, 2540
DriI GACNNNNNGTC 2 cut(s) 734, 1251
Eam1104I CTCTTC 1 cut(s) 149
Eam1105I GACNNNNNGTC 2 cut(s) 734, 1251
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 2286, 2905
Eco24I GRGCYC 1 cut(s) 103
Eco31I GGTCTC 2 cut(s) 742, 1007
Eco47I GGWCC 5 cut(s) 350, 769, 1362, 1942, 3059
Eco47III AGCGCT 1 cut(s) 495
Eco57I CTGAAG 7 cut(s) 375, 411, 1179, 1245, 1527, 1809, 1818
Eco91I GGTNACC 1 cut(s) 1230
EcoNI CCTNNNNNAGG 1 cut(s) 1478
EcoO109I RGGNCCY 3 cut(s) 350, 1362, 1942
EcoO65I GGTNACC 1 cut(s) 1230
EcoRII CCWGG 6 cut(s) 168, 713, 1542, 1954, 2148, 2775
EcoT14I CCWWGG 2 cut(s) 2286, 2905
EcoT22I ATGCAT 1 cut(s) 3008
EcoT38I GRGCYC 1 cut(s) 103
ErhI CCWWGG 2 cut(s) 2286, 2905
Esp3I CGTCTC 1 cut(s) 1313
FaqI GGGAC 4 cut(s) 96, 607, 1375, 2394
FauI CCCGC 1 cut(s) 242
FbaI TGATCA 4 cut(s) 117, 2448, 2523, 2889
FblI GTMKAC 1 cut(s) 2062
Fnu4HI GCNGC 5 cut(s) 207, 724, 1311, 1989, 3047
FokI GGATG 7 cut(s) 503, 728, 731, 1022, 2249, 2387, 2666
FriOI GRGCYC 1 cut(s) 103
Fsp4HI GCNGC 5 cut(s) 207, 724, 1311, 1989, 3047
FspBI CTAG 3 cut(s) 1256, 2552, 2987
GlaI GCGC 3 cut(s) 495, 532, 2487
GluI GCNGC 5 cut(s) 207, 724, 1311, 1989, 3047
GsuI CTGGAG 1 cut(s) 851
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 4 cut(s) 1484, 1954, 2132, 2721
HapII CCGG 3 cut(s) 819, 1946, 3011
HhaI GCGC 3 cut(s) 496, 533, 2488
Hin6I GCGC 3 cut(s) 494, 531, 2486
HinP1I GCGC 3 cut(s) 494, 531, 2486
HincII GTYRAC 4 cut(s) 62, 1390, 1603, 2194
HindII GTYRAC 4 cut(s) 62, 1390, 1603, 2194
HindIII AAGCTT 2 cut(s) 847, 2611
HpaII CCGG 3 cut(s) 819, 1946, 3011
Hpy99I CGWCG 2 cut(s) 49, 108
HpyCH4III ACNGT 7 cut(s) 446, 952, 2002, 2431, 2875, 2926, 3026
HpyCH4IV ACGT 6 cut(s) 400, 479, 1088, 1599, 1702, 2383
HpyF10VI GCNNNNNNNGC 5 cut(s) 661, 855, 898, 1838, 2947
HpySE526I ACGT 6 cut(s) 400, 479, 1088, 1599, 1702, 2383
HspAI GCGC 3 cut(s) 494, 531, 2486
Kpn2I TCCGGA 1 cut(s) 818
Ksp22I TGATCA 4 cut(s) 117, 2448, 2523, 2889
LmnI GCTCC 3 cut(s) 1888, 2257, 2909
Lsp1109I GCAGC 5 cut(s) 218, 735, 1297, 1975, 3033
MaeI CTAG 3 cut(s) 1256, 2552, 2987
MaeII ACGT 6 cut(s) 400, 479, 1088, 1599, 1702, 2383
MfeI CAATTG 2 cut(s) 1191, 2733
MflI RGATCY 6 cut(s) 165, 600, 1539, 1870, 1894, 2480
MhlI GDGCHC 3 cut(s) 103, 235, 1294
MluCI AATT 6 cut(s) 1191, 1274, 1574, 2107, 2733, 2748
MlyI GAGTC 5 cut(s) 78, 851, 1843, 3026, 3062
MmeI TCCRAC 3 cut(s) 739, 1912, 2857
Mph1103I ATGCAT 1 cut(s) 3008
MroI TCCGGA 1 cut(s) 818
MroXI GAANNNNTTC 4 cut(s) 495, 570, 1776, 1971
MslI CAYNNNNRTG 5 cut(s) 521, 1689, 2498, 2525, 3033
MspA1I CMGCKG 1 cut(s) 800
MspCI CTTAAG 1 cut(s) 1249
MspI CCGG 3 cut(s) 819, 1946, 3011
MspR9I CCNGG 7 cut(s) 170, 715, 1544, 1946, 1956, 2150, 2777
MunI CAATTG 2 cut(s) 1191, 2733
Mva1269I GAATGC 1 cut(s) 2326
MvaI CCWGG 6 cut(s) 170, 715, 1544, 1956, 2150, 2777
MwoI GCNNNNNNNGC 5 cut(s) 661, 855, 898, 1838, 2947
NciI CCSGG 1 cut(s) 1946
NcoI CCATGG 1 cut(s) 2286
NlaIV GGNNCC 5 cut(s) 232, 910, 1363, 1944, 2971
NmeAIII GCCGAG 2 cut(s) 1640, 2655
NmuCI GTSAC 7 cut(s) 644, 709, 943, 1243, 1595, 2011, 2227
NsiI ATGCAT 1 cut(s) 3008
NspI RCATGY 3 cut(s) 586, 878, 1317
NspV TTCGAA 1 cut(s) 2826
OliI CACNNNNGTG 1 cut(s) 2498
PagI TCATGA 1 cut(s) 319
PaqCI CACCTGC 1 cut(s) 1015
PciI ACATGT 1 cut(s) 874
PcsI WCGNNNNNNNCGW 2 cut(s) 103, 109
PctI GAATGC 1 cut(s) 2326
PdmI GAANNNNTTC 4 cut(s) 495, 570, 1776, 1971
PfeI GAWTC 9 cut(s) 40, 360, 1760, 1772, 1935, 1995, 2726, 2880, 2983
PflMI CCANNNNNTGG 3 cut(s) 751, 803, 2155
PfoI TCCNGGA 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 3010
PkrI GCNGC 5 cut(s) 208, 725, 1312, 1990, 3048
PleI GAGTC 5 cut(s) 78, 850, 1843, 3025, 3062
PpsI GAGTC 5 cut(s) 78, 850, 1843, 3025, 3062
Ppu21I YACGTR 1 cut(s) 1703
PpuMI RGGWCCY 3 cut(s) 350, 1362, 1942
PscI ACATGT 1 cut(s) 874
PshBI ATTAAT 1 cut(s) 1277
Psp1406I AACGTT 1 cut(s) 479
Psp5II RGGWCCY 3 cut(s) 350, 1362, 1942
Psp6I CCWGG 6 cut(s) 168, 713, 1542, 1954, 2148, 2775
PspEI GGTNACC 1 cut(s) 1230
PspGI CCWGG 6 cut(s) 168, 713, 1542, 1954, 2148, 2775
PspN4I GGNNCC 5 cut(s) 232, 910, 1363, 1944, 2971
PspPI GGNCC 7 cut(s) 350, 769, 1362, 1942, 2130, 2720, 3059
PspPPI RGGWCCY 3 cut(s) 350, 1362, 1942
PsrI GAACNNNNNNTAC 2 cut(s) 1611, 1643
PstI CTGCAG 1 cut(s) 1026
PstNI CAGNNNCTG 2 cut(s) 1543, 2231
PsuI RGATCY 6 cut(s) 165, 600, 1539, 1870, 1894, 2480
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 3 cut(s) 399, 1221, 1701
RsaNI GTAC 3 cut(s) 398, 1220, 1700
RseI CAYNNNNRTG 5 cut(s) 521, 1689, 2498, 2525, 3033
SatI GCNGC 5 cut(s) 207, 724, 1311, 1989, 3047
Sau96I GGNCC 7 cut(s) 350, 769, 1362, 1942, 2130, 2720, 3059
SchI GAGTC 5 cut(s) 78, 851, 1843, 3026, 3062
ScrFI CCNGG 7 cut(s) 170, 715, 1544, 1946, 1956, 2150, 2777
SduI GDGCHC 3 cut(s) 103, 235, 1294
SfcI CTRYAG 4 cut(s) 534, 1022, 1554, 2085
SfuI TTCGAA 1 cut(s) 2826
SinI GGWCC 5 cut(s) 350, 769, 1362, 1942, 3059
SmiMI CAYNNNNRTG 5 cut(s) 521, 1689, 2498, 2525, 3033
SmlI CTYRAG 3 cut(s) 1249, 1631, 2200
SmoI CTYRAG 3 cut(s) 1249, 1631, 2200
Sse9I AATT 6 cut(s) 1191, 1274, 1574, 2107, 2733, 2748
SsiI CCGC 2 cut(s) 235, 2496
SspMI CTAG 3 cut(s) 1256, 2552, 2987
StyD4I CCNGG 7 cut(s) 168, 713, 1542, 1944, 1954, 2148, 2775
StyI CCWWGG 2 cut(s) 2286, 2905
TaaI ACNGT 7 cut(s) 446, 952, 2002, 2431, 2875, 2926, 3026
TaiI ACGT 6 cut(s) 403, 482, 1091, 1602, 1705, 2386
TaqI TCGA 7 cut(s) 24, 129, 285, 404, 682, 1638, 2826
TaqII GACCGA 2 cut(s) 2151, 2573
TasI AATT 6 cut(s) 1191, 1274, 1574, 2107, 2733, 2748
TfiI GAWTC 9 cut(s) 40, 360, 1760, 1772, 1935, 1995, 2726, 2880, 2983
TseFI GTSAC 7 cut(s) 644, 709, 943, 1243, 1595, 2011, 2227
TseI GCWGC 5 cut(s) 206, 723, 1310, 1988, 3046
Tsp45I GTSAC 7 cut(s) 644, 709, 943, 1243, 1595, 2011, 2227
TspDTI ATGAA 6 cut(s) 1283, 1893, 2136, 2597, 2605, 2802
TspGWI ACGGA 8 cut(s) 33, 67, 101, 1364, 1782, 1883, 2093, 2962
Van91I CCANNNNNTGG 3 cut(s) 751, 803, 2155
Vha464I CTTAAG 1 cut(s) 1249
VpaK11BI GGWCC 5 cut(s) 350, 769, 1362, 1942, 3059
VspI ATTAAT 1 cut(s) 1277
XagI CCTNNNNNAGG 1 cut(s) 1478
XapI RAATTY 1 cut(s) 1574
XceI RCATGY 3 cut(s) 586, 878, 1317
XcmI CCANNNNNNNNNTGG 3 cut(s) 748, 779, 1378
XmiI GTMKAC 1 cut(s) 2062
XmnI GAANNNNTTC 4 cut(s) 495, 570, 1776, 1971
XspI CTAG 3 cut(s) 1256, 2552, 2987
Zsp2I ATGCAT 1 cut(s) 3008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.