Rh3CG288700

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002172
Physical Location & Seq
Forward (+)
29240776 .. 29287875
47100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG288700.1

Sequence Viewer

Length: 2526 bp
ATGGAAAGCTACTTGCACAAGGACTTCGAAGTCCAGCACAAGCACCTCTCGCCGGAGGCCTCAAGCAATTGGAGGAAAGCCGTCGGCCGCGTCGTCAAGAACCCCGCCAGGAGGTTCCGGCATGTCGCCGATCTTGACAAGCGCGCCGAGGCTGAGAGCAAGAATAAGCAAATCCAGCAGGAAAAAATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTAACATTCATTGAGGCTGGAGGTAGTCAACCAGTTACTGATCAGGTTGAATACAAACTGTCTGAAGAGGCCGAAAAAGAGGGTTTCAGCATTCACCCAGATGAACTTGCATCAATCATACGCAGCCATGATATTAAGGTCTTGAAAAGATATGGTGGAGTTGATGGGATTTTAACAAAAGTATCCGTAGTACCAGATGAAGGTGTCAGGAGTAGCAGTATACCAATAAGGCAAAATGTTTATGGCTTAAACCGCTATACGGAGAAACCTCCCCGATCATTCTTGGTGTTTGTATGGGAAGCTCTACAGGATTTAACTTTAATTATTCTTATGGTTTGTGCCGTGGTTTCAATTGGAGTGGGACTTGCTACTGACGGGTGGCCAGAAGGCATGTATGATGGGGTGGGAATTTTACTCAGTATAGCTTTAGTTGTTTTGGTCACTGCCATTAGTGATTATAGGCAGTCCATGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTATTCAGGTGACTAGGGATGGGAAAAGACAAAAAGTTTCCATTTATGATTTGCTGGTGGGAGATATTGTTCATTTGGCAGTGGGTGACCTTGTTCCAGCAGATGGACTTTTCATATCAGGATACAGCTTGTTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGAATGTGTATGAAGAAAAGCCTTTTCTTCTTTCGGGAACCAAAGTGCAAGATGGGTCAGGGAAAATGCTGGTAACAACAGTTGGTATGAGGACCGAATGGGGAAAGTTGATGGAAACTCTGAGTGAGGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGGCTTTCGCTGTGGTGACATTTTTGGTTTTGACCGTAAGATATTTGGTGGAAAAAGCACTTAGCAATGAGATCACAGATTGGTCTTCAACTGATGCTGTAACACTTTTGAACTACTTTGCCATTGCAGTAACTATAATTGTTGTTGCAGTTCCTGAAGGTTTACCATTGGCAGTAACACTGAGTCTTGCTTTTGCAATGAAGAAATTAATGAATGATAAGGCACTTGTGAGGCATCTCTCAGCCTGTGAGACAATGGGTTCTGCTAGTTGCATATGCACGGATAAAACAGGAACATTAACCACAAACCATATGGTAGTTACCAAGATATGGATAGGTGACAAATCTATAAATGTAAAAGGTAATGAAAGTAAAGACGTAGTAAAATCAGAAATTTCTGGAGCACTGGACATCCTATTGCAGGTTATATTTCAAAATACAAGCTCTGAGGTTATCAAGGATGAGGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCATTATTAGAATTTGGTTTGCTTTTGGGTGGCGATTTTGATGCCCTGCGCAGAGAGTTTAAGATTATTAAGATGGAACCTTTCAATTCTGTTAGGAAGAAGATGTCTGTGCTCATCGCTCATCCTGATGGTGGGATAAGAGCTTTTTGCAAAGGTGCATCTGAAATAGTATTGGGAATGTGCAACAAAGTTATTGACTGTAATGGAGAATCTGTTGATCTCTCCCGAAAAGAGGCAAATAATATCACAGATGTCATAAATTCTTTTGCTTCTGAAGCATTAAGAACTCTCTGCTTGGCTTTTAAAGATATACATGACTCTTCCATCAACAATGACATCCCCAATGATGGCTATACATTGATAGCAGTTGTAGGAATCAAGGACCCTGTGCGTCCTGGGGTGAAGGAAGCAGTCCAAACTTGTTTGGCTGCTGGGATAACCGTCCGAATGGTCACAGGTGACAATATAAATACTGCTAAAGCCATAGCTAGAGAATGTGGCATACTCACAGAGGATGGTTTGGCAATAGAAGGACCAGAATTTCGTAACATGTCTCCTGCAGATATGAAAGCTGTTATACCGAGGATTCAGGTGATGGCCCGATCCTTACCATTGGATAAGCACACCTTGGTGAAGAATTTGAGGAATACATTCCGGGAGGTAGTTGCAGTTACAGGTGATGGGACCAATGACGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGCACAGAGGTTTGTGTCTTAGCTACTTCTCTAGTTACAATCTCAAATCTTGAGTACCTATCTATCACCTTGTTGCGCCCTTATGGGATCCTTTTTCTTTACCTTTTGTTTTTGTTTTTGTTCTTTTTTCTTTTCATTTTCTTTTTTAATTTCAACATCTGTTGCATAATGTTGTGCTTGCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

841

Amino Acids

92.13

Weight (kDa)

5.7

Isoelectric Point (pI)

33.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 5 - 50 2.1e-15 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 124 - 190 1.9e-12 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 245 - 339 8.1e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 514 - 664 2e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 651 - 771 6.8e-12 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1042
AasI GACNNNNNNGTC 1 cut(s) 29
Acc16I TGCGCA 1 cut(s) 1646
Acc36I ACCTGC 2 cut(s) 1042, 1507
AccB7I CCANNNNNTGG 2 cut(s) 830, 1425
AccI GTMKAC 1 cut(s) 439
AccII CGCG 2 cut(s) 90, 144
AciI CCGC 3 cut(s) 88, 105, 472
AclWI GGATC 3 cut(s) 2193, 2419, 2432
AcoI YGGCCR 2 cut(s) 85, 599
AcsI RAATTY 7 cut(s) 186, 627, 1488, 1607, 1855, 2135, 2233
AcuI CTGAAG 3 cut(s) 303, 1272, 1890
AfaI GTAC 2 cut(s) 411, 2393
AflIII ACRYGT 1 cut(s) 2145
AjnI CCWGG 2 cut(s) 107, 1990
AleI CACNNNNGTG 1 cut(s) 2225
AloI GAACNNNNNNTCC 2 cut(s) 780, 812
Alw21I GWGCWC 2 cut(s) 1501, 1710
Alw26I GTCTC 3 cut(s) 1034, 1340, 2154
AlwI GGATC 3 cut(s) 2193, 2419, 2432
AlwNI CAGNNNCTG 2 cut(s) 257, 1250
AoxI GGCC 5 cut(s) 57, 85, 288, 599, 2193
ApeKI GCWGC 3 cut(s) 215, 342, 2024
ApoI RAATTY 7 cut(s) 186, 627, 1488, 1607, 1855, 2135, 2233
ArsI GACNNNNNNTTYG 4 cut(s) 1558, 1590, 2121, 2153
AseI ATTAAT 1 cut(s) 1304
Asp700I GAANNNNTTC 2 cut(s) 186, 2246
AspLEI GCGC 4 cut(s) 144, 146, 1647, 2415
AspS9I GGNCC 5 cut(s) 990, 1978, 2129, 2194, 2280
AsuC2I CCSGG 1 cut(s) 2252
AsuII TTCGAA 1 cut(s) 27
AvaII GGWCC 4 cut(s) 990, 1978, 2129, 2280
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BalI TGGCCA 1 cut(s) 601
BamHI GGATCC 1 cut(s) 2424
BbsI GAAGAC 1 cut(s) 1173
Bbv12I GWGCWC 2 cut(s) 1501, 1710
BbvI GCAGC 3 cut(s) 227, 354, 2011
BceAI ACGGC 2 cut(s) 65, 545
BcgI CGANNNNNNTGC 2 cut(s) 1619, 1653
BciT130I CCWGG 2 cut(s) 109, 1992
BciVI GTATCC 2 cut(s) 412, 842
BclI TGATCA 1 cut(s) 259
BcnI CCSGG 1 cut(s) 2252
BcoDI GTCTC 3 cut(s) 1034, 1340, 2154
BfaI CTAG 4 cut(s) 741, 1362, 2085, 2369
BfmI CTRYAG 3 cut(s) 524, 1049, 2154
BfuAI ACCTGC 2 cut(s) 1042, 1507
BfuI GTATCC 2 cut(s) 412, 842
BisI GCNGC 4 cut(s) 88, 216, 343, 2025
BlsI GCNGC 4 cut(s) 89, 217, 344, 2026
Bme1390I CCNGG 3 cut(s) 109, 1992, 2252
Bme18I GGWCC 4 cut(s) 990, 1978, 2129, 2280
BmgT120I GGNCC 5 cut(s) 990, 1978, 2129, 2194, 2280
BmiI GGNNCC 6 cut(s) 116, 937, 1674, 1980, 2281, 2426
BmrFI CCNGG 3 cut(s) 109, 1992, 2252
BmsI GCATC 5 cut(s) 338, 1180, 1339, 1627, 1763
BpiI GAAGAC 1 cut(s) 1173
BpmI CTGGAG 2 cut(s) 258, 1515
Bpu14I TTCGAA 1 cut(s) 27
BpuEI CTTGAG 2 cut(s) 46, 2408
BpuMI CCSGG 1 cut(s) 2252
BsaI GGTCTC 1 cut(s) 1034
BsaJI CCNNGG 5 cut(s) 147, 561, 1991, 2177, 2223
BsaXI ACNNNNNCTCC 6 cut(s) 421, 451, 780, 810, 1794, 1824
Bse1I ACTGG 3 cut(s) 251, 896, 1506
Bse3DI GCAATG 3 cut(s) 1168, 1218, 1299
BseBI CCWGG 2 cut(s) 109, 1992
BseDI CCNNGG 5 cut(s) 147, 561, 1991, 2177, 2223
BseGI GGATG 6 cut(s) 751, 1506, 1561, 1717, 1932, 2116
BseMI GCAATG 3 cut(s) 1168, 1218, 1299
BseMII CTCAG 6 cut(s) 144, 649, 1010, 1268, 1350, 1533
BseNI ACTGG 3 cut(s) 251, 896, 1506
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 1044
BseX3I CGGCCG 1 cut(s) 85
BseXI GCAGC 3 cut(s) 227, 354, 2011
BseYI CCCAGC 1 cut(s) 2027
Bsh1236I CGCG 2 cut(s) 90, 144
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 5 cut(s) 59, 87, 290, 601, 2195
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 1501, 1710
BsiSI CCGG 3 cut(s) 53, 118, 2251
BslFI GGGAC 2 cut(s) 594, 2293
BsmAI GTCTC 3 cut(s) 1034, 1340, 2154
BsmFI GGGAC 2 cut(s) 594, 2293
BsmI GAATGC 1 cut(s) 309
BsnI GGCC 5 cut(s) 59, 87, 290, 601, 2195
Bso31I GGTCTC 1 cut(s) 1034
Bsp119I TTCGAA 1 cut(s) 27
Bsp1286I GDGCHC 2 cut(s) 1501, 1710
Bsp143I GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
BspACI CCGC 3 cut(s) 88, 105, 472
BspANI GGCC 5 cut(s) 59, 87, 290, 601, 2195
BspCNI CTCAG 6 cut(s) 145, 648, 1011, 1269, 1349, 1534
BspFNI CGCG 2 cut(s) 90, 144
BspLI GGNNCC 6 cut(s) 116, 937, 1674, 1980, 2281, 2426
BspMAI CTGCAG 2 cut(s) 1053, 2158
BspMI ACCTGC 2 cut(s) 1042, 1507
BspPI GGATC 3 cut(s) 2193, 2419, 2432
BspT104I TTCGAA 1 cut(s) 27
BspTNI GGTCTC 1 cut(s) 1034
BsrDI GCAATG 3 cut(s) 1168, 1218, 1299
BsrI ACTGG 3 cut(s) 251, 896, 1506
BssECI CCNNGG 5 cut(s) 147, 561, 1991, 2177, 2223
BssHII GCGCGC 1 cut(s) 142
BssMI GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
BssNAI GTATAC 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 2223
Bst1107I GTATAC 1 cut(s) 440
Bst2UI CCWGG 2 cut(s) 109, 1992
Bst4CI ACNGT 5 cut(s) 279, 979, 1132, 1796, 2038
Bst6I CTCTTC 2 cut(s) 279, 1921
BstAPI GCANNNNNTGC 1 cut(s) 688
BstBI TTCGAA 1 cut(s) 27
BstC8I GCNNGC 3 cut(s) 144, 2338, 2516
BstDEI CTNAG 8 cut(s) 153, 635, 1019, 1157, 1277, 1336, 1542, 2356
BstDSI CCRYGG 1 cut(s) 561
BstEII GGTNACC 1 cut(s) 812
BstENI CCTNNNNNAGG 1 cut(s) 1514
BstF5I GGATG 6 cut(s) 751, 1506, 1561, 1717, 1932, 2116
BstFNI CGCG 2 cut(s) 90, 144
BstHHI GCGC 4 cut(s) 144, 146, 1647, 2415
BstKTI GATC 7 cut(s) 133, 262, 497, 1170, 1816, 2201, 2427
BstMAI GTCTC 3 cut(s) 1034, 1340, 2154
BstMBI GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 7 cut(s) 49, 175, 471, 688, 1057, 1871, 2337
BstNI CCWGG 2 cut(s) 109, 1992
BstNSI RCATGY 3 cut(s) 125, 613, 2149
BstPI GGTNACC 1 cut(s) 812
BstSCI CCNGG 3 cut(s) 107, 1990, 2250
BstSFI CTRYAG 3 cut(s) 524, 1049, 2154
BstUI CGCG 2 cut(s) 90, 144
BstV1I GCAGC 3 cut(s) 227, 354, 2011
BstV2I GAAGAC 1 cut(s) 1173
BstX2I RGATCY 1 cut(s) 2424
BstYI RGATCY 1 cut(s) 2424
BstZ17I GTATAC 1 cut(s) 440
BstZI CGGCCG 1 cut(s) 85
BsuI GTATCC 2 cut(s) 412, 842
BsuRI GGCC 5 cut(s) 59, 87, 290, 601, 2195
BtgI CCRYGG 1 cut(s) 561
BtgZI GCGATG 1 cut(s) 1696
BtsCI GGATG 6 cut(s) 751, 1506, 1561, 1717, 1932, 2116
BtsI GCAGTG 3 cut(s) 660, 813, 1046
BtsIMutI CAGTG 6 cut(s) 660, 813, 903, 1046, 1274, 1499
BveI ACCTGC 2 cut(s) 1042, 1507
Cac8I GCNNGC 3 cut(s) 144, 2338, 2516
CaiI CAGNNNCTG 2 cut(s) 257, 1250
CfoI GCGC 4 cut(s) 144, 146, 1647, 2415
Cfr13I GGNCC 5 cut(s) 990, 1978, 2129, 2194, 2280
CseI GACGC 3 cut(s) 79, 1976, 2297
Csp6I GTAC 2 cut(s) 410, 2392
CspCI CAANNNNNGTGG 2 cut(s) 558, 593
CviAII CATG 7 cut(s) 122, 347, 610, 688, 1910, 2146, 2303
CviQI GTAC 2 cut(s) 410, 2392
DdeI CTNAG 8 cut(s) 153, 635, 1019, 1157, 1277, 1336, 1542, 2356
DpnI GATC 7 cut(s) 132, 261, 496, 1169, 1815, 2200, 2426
DpnII GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
DraI TTTAAA 1 cut(s) 1900
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 2 cut(s) 85, 599
EagI CGGCCG 1 cut(s) 85
Eam1104I CTCTTC 2 cut(s) 279, 1921
EarI CTCTTC 2 cut(s) 279, 1921
EclXI CGGCCG 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 2223
Eco147I AGGCCT 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 1034
Eco47I GGWCC 4 cut(s) 990, 1978, 2129, 2280
Eco52I CGGCCG 1 cut(s) 85
Eco57I CTGAAG 3 cut(s) 303, 1272, 1890
Eco91I GGTNACC 1 cut(s) 812
EcoNI CCTNNNNNAGG 1 cut(s) 1514
EcoO109I RGGNCCY 1 cut(s) 1978
EcoO65I GGTNACC 1 cut(s) 812
EcoRII CCWGG 2 cut(s) 107, 1990
EcoT14I CCWWGG 1 cut(s) 2223
ErhI CCWWGG 1 cut(s) 2223
FaeI CATG 7 cut(s) 125, 350, 613, 691, 1913, 2149, 2306
FalI AAGNNNNNCTT 2 cut(s) 2207, 2239
FaqI GGGAC 2 cut(s) 594, 2293
FatI CATG 7 cut(s) 121, 346, 609, 687, 1909, 2145, 2302
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 2 cut(s) 1370, 1407
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 1 cut(s) 439
Fnu4HI GCNGC 4 cut(s) 88, 216, 343, 2025
FokI GGATG 6 cut(s) 758, 1493, 1568, 1704, 1919, 2123
Fsp4HI GCNGC 4 cut(s) 88, 216, 343, 2025
FspBI CTAG 4 cut(s) 741, 1362, 2085, 2369
FspI TGCGCA 1 cut(s) 1646
GlaI GCGC 4 cut(s) 143, 145, 1646, 2414
GluI GCNGC 4 cut(s) 88, 216, 343, 2025
GsaI CCCAGC 1 cut(s) 2031
GsuI CTGGAG 2 cut(s) 258, 1515
HaeIII GGCC 5 cut(s) 59, 87, 290, 601, 2195
HapII CCGG 3 cut(s) 53, 118, 2251
HgaI GACGC 3 cut(s) 79, 1976, 2297
HhaI GCGC 4 cut(s) 144, 146, 1647, 2415
Hin1II CATG 7 cut(s) 125, 350, 613, 691, 1913, 2149, 2306
Hin6I GCGC 4 cut(s) 142, 144, 1645, 2413
HinP1I GCGC 4 cut(s) 142, 144, 1645, 2413
HincII GTYRAC 1 cut(s) 248
HindII GTYRAC 1 cut(s) 248
HindIII AAGCTT 1 cut(s) 874
HinfI GANTC 7 cut(s) 869, 1279, 1592, 1805, 1913, 1971, 2182
HpaII CCGG 3 cut(s) 53, 118, 2251
Hpy166II GTNNAC 3 cut(s) 248, 440, 1259
Hpy188I TCNGA 7 cut(s) 283, 1020, 1486, 1543, 1759, 1870, 2042
Hpy8I GTNNAC 3 cut(s) 248, 440, 1259
Hpy99I CGWCG 2 cut(s) 86, 95
HpyAV CCTTC 5 cut(s) 413, 599, 1247, 1993, 2120
HpyCH4III ACNGT 5 cut(s) 279, 979, 1132, 1796, 2038
HpyCH4IV ACGT 1 cut(s) 1473
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 175, 471, 688, 1057, 1871, 2337
HpyF3I CTNAG 8 cut(s) 153, 635, 1019, 1157, 1277, 1336, 1542, 2356
HpySE526I ACGT 1 cut(s) 1473
Hsp92II CATG 7 cut(s) 125, 350, 613, 691, 1913, 2149, 2306
HspAI GCGC 4 cut(s) 142, 144, 1645, 2413
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
LmnI GCTCC 2 cut(s) 1496, 2296
Lsp1109I GCAGC 3 cut(s) 227, 354, 2011
LweI GCATC 5 cut(s) 338, 1180, 1339, 1627, 1763
MaeI CTAG 4 cut(s) 741, 1362, 2085, 2369
MaeII ACGT 1 cut(s) 1473
MalI GATC 7 cut(s) 132, 261, 496, 1169, 1815, 2200, 2426
MboI GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
MfeI CAATTG 2 cut(s) 67, 570
MflI RGATCY 1 cut(s) 2424
MhlI GDGCHC 2 cut(s) 1501, 1710
MlsI TGGCCA 1 cut(s) 601
MluNI TGGCCA 1 cut(s) 601
MlyI GAGTC 4 cut(s) 878, 1288, 1601, 1907
Mox20I TGGCCA 1 cut(s) 601
MroXI GAANNNNTTC 2 cut(s) 186, 2246
MscI TGGCCA 1 cut(s) 601
MslI CAYNNNNRTG 3 cut(s) 318, 1722, 2225
Msp20I TGGCCA 1 cut(s) 601
MspI CCGG 3 cut(s) 53, 118, 2251
MspR9I CCNGG 3 cut(s) 109, 1992, 2252
MunI CAATTG 2 cut(s) 67, 570
Mva1269I GAATGC 1 cut(s) 309
MvaI CCWGG 2 cut(s) 109, 1992
MvnI CGCG 2 cut(s) 90, 144
MwoI GCNNNNNNNGC 7 cut(s) 49, 175, 471, 688, 1057, 1871, 2337
NciI CCSGG 1 cut(s) 2252
NdeI CATATG 2 cut(s) 1370, 1407
NdeII GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
NlaIII CATG 7 cut(s) 125, 350, 613, 691, 1913, 2149, 2306
NlaIV GGNNCC 6 cut(s) 116, 937, 1674, 1980, 2281, 2426
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 7 cut(s) 658, 736, 812, 1111, 1433, 2047, 2054
NsbI TGCGCA 1 cut(s) 1646
NspI RCATGY 3 cut(s) 125, 613, 2149
NspV TTCGAA 1 cut(s) 27
OliI CACNNNNGTG 1 cut(s) 2225
PaqCI CACCTGC 1 cut(s) 1042
PauI GCGCGC 1 cut(s) 142
PceI AGGCCT 1 cut(s) 59
PciI ACATGT 1 cut(s) 2145
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 309
PdmI GAANNNNTTC 2 cut(s) 186, 2246
PfeI GAWTC 3 cut(s) 1805, 1971, 2182
PflMI CCANNNNNTGG 2 cut(s) 830, 1425
PfoI TCCNGGA 1 cut(s) 2250
PkrI GCNGC 4 cut(s) 89, 217, 344, 2026
PleI GAGTC 4 cut(s) 877, 1287, 1600, 1907
PpsI GAGTC 4 cut(s) 877, 1287, 1600, 1907
PpuMI RGGWCCY 1 cut(s) 1978
PscI ACATGT 1 cut(s) 2145
PshBI ATTAAT 1 cut(s) 1304
Psp5II RGGWCCY 1 cut(s) 1978
Psp6I CCWGG 2 cut(s) 107, 1990
PspEI GGTNACC 1 cut(s) 812
PspFI CCCAGC 1 cut(s) 2027
PspGI CCWGG 2 cut(s) 107, 1990
PspN4I GGNNCC 6 cut(s) 116, 937, 1674, 1980, 2281, 2426
PspPI GGNCC 5 cut(s) 990, 1978, 2129, 2194, 2280
PspPPI RGGWCCY 1 cut(s) 1978
PstI CTGCAG 2 cut(s) 1053, 2158
PstNI CAGNNNCTG 2 cut(s) 257, 1250
PsuI RGATCY 1 cut(s) 2424
PteI GCGCGC 1 cut(s) 142
RsaI GTAC 2 cut(s) 411, 2393
RsaNI GTAC 2 cut(s) 410, 2392
RseI CAYNNNNRTG 3 cut(s) 318, 1722, 2225
SatI GCNGC 4 cut(s) 88, 216, 343, 2025
Sau3AI GATC 7 cut(s) 130, 259, 494, 1167, 1813, 2198, 2424
Sau96I GGNCC 5 cut(s) 990, 1978, 2129, 2194, 2280
SchI GAGTC 4 cut(s) 878, 1288, 1601, 1907
ScrFI CCNGG 3 cut(s) 109, 1992, 2252
SduI GDGCHC 2 cut(s) 1501, 1710
SfaNI GCATC 5 cut(s) 338, 1180, 1339, 1627, 1763
SfcI CTRYAG 3 cut(s) 524, 1049, 2154
SfuI TTCGAA 1 cut(s) 27
SinI GGWCC 4 cut(s) 990, 1978, 2129, 2280
SmiMI CAYNNNNRTG 3 cut(s) 318, 1722, 2225
SmlI CTYRAG 2 cut(s) 61, 2387
SmoI CTYRAG 2 cut(s) 61, 2387
SseBI AGGCCT 1 cut(s) 59
SsiI CCGC 3 cut(s) 88, 105, 472
SspMI CTAG 4 cut(s) 741, 1362, 2085, 2369
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 3 cut(s) 107, 1990, 2250
StyI CCWWGG 1 cut(s) 2223
TaaI ACNGT 5 cut(s) 279, 979, 1132, 1796, 2038
TaiI ACGT 1 cut(s) 1476
TaqI TCGA 2 cut(s) 27, 190
TaqII GACCGA 1 cut(s) 1007
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 3 cut(s) 1805, 1971, 2182
TscAI CASTG 6 cut(s) 667, 813, 903, 1053, 1281, 1506
TseFI GTSAC 7 cut(s) 658, 736, 812, 1111, 1433, 2047, 2054
TseI GCWGC 3 cut(s) 215, 342, 2024
Tsp45I GTSAC 7 cut(s) 658, 736, 812, 1111, 1433, 2047, 2054
TspGWI ACGGA 3 cut(s) 394, 494, 1391
TspRI CASTG 6 cut(s) 667, 813, 903, 1053, 1281, 1506
Van91I CCANNNNNTGG 2 cut(s) 830, 1425
VpaK11BI GGWCC 4 cut(s) 990, 1978, 2129, 2280
VspI ATTAAT 1 cut(s) 1304
XagI CCTNNNNNAGG 1 cut(s) 1514
XapI RAATTY 7 cut(s) 186, 627, 1488, 1607, 1855, 2135, 2233
XceI RCATGY 3 cut(s) 125, 613, 2149
XcmI CCANNNNNNNNNTGG 1 cut(s) 1405
XmiI GTMKAC 1 cut(s) 439
XmnI GAANNNNTTC 2 cut(s) 186, 2246
XspI CTAG 4 cut(s) 741, 1362, 2085, 2369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.