RchiOBHm_Chr3g0483421

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002172
Physical Location & Seq
Forward (+)
29698493 .. 29706329
7837 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44817

Sequence Viewer

Length: 3114 bp
ATGGAAAGCTACTTGCACAAGGACTTCAAAATCCAGCCCAAGCACCGCTCGCCGGAGGCTTCAAGAAACTGGAAGAAAGCCGTCGGCCTCATCGTCAAGAACCGGGCCAGAAGGTTCCGACATGTCCCCGATCCCGACAAGCGCGCCGAGGGTGAGAGGAAGAAGAAGGAAATCCAGGAAAAAATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTAACATTCATTGAGGCTGGAGGTAGTCAACCAGTTACTGATCAGGTTGAATACAAACTGTCTGAAGAGGCCGAAAAAGAGGGTTTCAGCATTCACCCAGATGAACTTGCATCAATCATACGCAGCCATGATATTAAGGTCTTGAAAAGACACGGTGGAGTTTATGGGATTTTAATAAAAGTATCCGTAGTACTAGATGAAGGTGTCAGGAGTAGCAGTATACCAACAAGGCAAAATGTTTATGGCTTAAACCGCTATACGGAGAAACCTCCCCGATCATTCTTGGTGTTTGTATGGGAAGCTCTACAGGATTTAACTTTAATTATTCTTATGGTTTGTGCCGTGGTTTCAATTGGAGTGGGACTTGCTACTGACGGGTGGCCAGAAGGCATGTATGATGGGGTGGGAATTTTACTCAGTATAGCTTTAGTTGTTTTGGTCACTGCCATTAGTGATTACAGGCAGTCCATGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTATTCAGGTCACTAGGGATGGGAAAAGACAAAAAGTTTCCATTTATGATTTGCTGGTGGGAGATATTGTTCATTTAGCAGTGGGTGACCTTGTTCCAGCAGATGGACTTTTCATATCAGGATACAGCTTGTTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGAATGTGTATGAAGAAAAGCCTTTTCTTCTTTCGGGAACCAAAGTGCAAGATGGGTCAGGGAAAATGCTGGTAACAACAGTTGGTATGAGGACCGAATGGGGAAAGTTGATGGAAACCCTGAGTGAGGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGGCTTTCGCTGTGGTGACATTTTTGGTTTTGACAGTAAGATATTTGGTGGAAAAAGCACTTAGCAATGAGATCACAGATTGGTCTTCAACTGATGCTGTAACACTTTTGAACTACTTTGCCATTGCAGTAACTATAATTGTTGTTGCAGTTCCTGAAGGTTTACCATTGGCAGTAACACTGAGTCTTGCTTTTGCAATGAAGAAATTAATGAATGATAAGGCACTTGTGAGGCATCTCTCAGCCTGTGAGACAATGGGTTCTGCTAGTTGCATATGCACGGATAAAACAGGAACATTAACCACAAACCATATGGTAGTTACCAAGATATGGATAGGTGACAAATCTATAAATGTAAAAGGTAATGAAAGTAAAGACGTAGTAAAATCAGAAATTTCTGGAGCACTGGACATCCTATTGCAGGTTATATTTCAAAATACAAGCTCTGAGGTTATCAAGGATGAGGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCATTATTAGAATTTGGTTTGCTTTTGGGTGGCGATTTTGATGCCCTGCGCAGAGAGTTTAAGATTATTAAGATGGAACCTTTCAATTCTGTTAGGAAGAAGATGTCTGTGCTCATCGCTCATCCTGATGGTGGGATAAGAGCTTTTTGCAAAGGTGCATCTGAAATAGTATTGGGAATGTGCAACAAAGTTATTGACTGTAATGGAGAATCTGTTGATCTCTCCCAAAAAGAGGCAAATAATATCACAGATGTCATAAATTCTTTTGCTTCTGAAGCATTAAGAACTCTCTGCTTGGCTTTTAAAGATATACATGACTCTTCCATCAACAATGACATCCCCAATGATGGCTACACATTGATAGCAGTTGTAGGAATCAAGGACCCTGTGCGTCCTGGGGTGAAGGAAGCAGTCCAAACTTGTTTGGCTGCTGGGATAACTGTCCGAATGGTCACAGGTGACAATATAAATACTGCTAAAGCCATAGCTAGAGAATGTGGCATACTCACAGAGGATGGTTTGGCAATAGAAGGACCAGAATTTCGTAACATGTCTCCTGCAGATATGAAAGCTGTTATACCGAGGATTCAGGTGATGGCCCGATCCTTACCATTGGATAAGCACACCTTGGTGAAGAATTTGAGGAATACATTCCAGGAGGTAGTTGCAGTTACAGGTGATGGGACCAATGACGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGCACAGAGGTTGCTAAAGAAAGTGCTGATGTCATCGTATTGGATGACAATTTTAGTACTGTAGTAAATGTGGCCAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTCCAGTTCCAGTTAACGGTTAACGTTGTTGCTCTGATGATCAATTTTGTTTCTGCATGTGTATCAGGAGATGCTCCCCTTACTGCTGTGCAATTACTCTGGGTCAACATGATTATGGACACTCTTGGTGCTTTGGCACTGGCTACAGAGCCTCCAAATGAAGGACTGATGAAAAGGCCCCCTGTTTCAAGGGGTACAAGCTTCATCACCAGGCCCATGTGGAGGAATATCATTGGTCAGAGCATTTATCAATTGGCTGTTCTTGGAGTTCTCGATTTCCGTGGAACGCAGCTGCTAGGACTCACTGGTTCAGATGCAACTGATATTCTTGACACTGTGATATTCAATGCATTCGTGTTCTGCCAGGTGTTCAATCAGATAAACAGCCGTGATATAGAGAAGATAAACATATTCCGTGGAATGTTTGACAGCTGGGTATTCCTAGGGGTCATGGTTTGCACAGTGGGCTTCCAAATTGTCCTAGTAGAGTTCTTGGGCGCGTTTGCTAGCACTGTACCACTGAGCTGGCAACTATGGTTACTCTGCATTATACTTGGATTGATTAGCATGCCTGTTGCAGTTGTCGTGAAATGCATACCAGTTGAAAGCAAAGTTAAAACCCCTGATGGCTATGAAGCTATCCCTGATGGTCCTGGCCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1037

Amino Acids

113.19

Weight (kDa)

5.7

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 5 - 50 6.6e-12 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 123 - 189 4.1e-11 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 244 - 338 1.1e-20 P-type ATPase actuator domain
Cation_ATPase PF13246 513 - 663 1.4e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 650 - 770 1.1e-11 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1014 1.7e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1039
Acc16I TGCGCA 1 cut(s) 1643
Acc36I ACCTGC 2 cut(s) 1039, 1504
AccB7I CCANNNNNTGG 2 cut(s) 827, 1422
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 1 cut(s) 436
AccII CGCG 2 cut(s) 144, 2948
AciI CCGC 2 cut(s) 46, 469
AclI AACGTT 1 cut(s) 2472
AclWI GGATC 2 cut(s) 125, 2190
AcoI YGGCCR 2 cut(s) 596, 2406
AcsI RAATTY 7 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2230
AcuI CTGAAG 3 cut(s) 300, 1269, 1887
AfaI GTAC 5 cut(s) 408, 2392, 2429, 2644, 2964
AflIII ACRYGT 2 cut(s) 121, 2142
AjnI CCWGG 6 cut(s) 174, 1987, 2247, 2657, 2811, 3100
AleI CACNNNNGTG 1 cut(s) 2222
AloI GAACNNNNNNTCC 2 cut(s) 777, 809
Alw21I GWGCWC 2 cut(s) 1498, 1707
Alw26I GTCTC 3 cut(s) 1031, 1337, 2151
AlwI GGATC 2 cut(s) 125, 2190
AlwNI CAGNNNCTG 2 cut(s) 254, 1247
AoxI GGCC 9 cut(s) 85, 105, 285, 596, 2190, 2406, 2624, 2660, 3103
ApeKI GCWGC 5 cut(s) 212, 339, 2021, 2737, 2740
ApoI RAATTY 7 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2230
ArsI GACNNNNNNTTYG 4 cut(s) 1555, 1587, 2118, 2150
AseI ATTAAT 1 cut(s) 1301
Asp700I GAANNNNTTC 2 cut(s) 183, 2243
AspA2I CCTAGG 1 cut(s) 2890
AspLEI GCGC 4 cut(s) 144, 146, 1644, 2948
AspS9I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
AsuC2I CCSGG 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 2954
AvaII GGWCC 5 cut(s) 987, 1975, 2126, 2277, 3098
AvrII CCTAGG 1 cut(s) 2890
BalI TGGCCA 2 cut(s) 598, 2408
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 2 cut(s) 1498, 1707
BbvI GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
BceAI ACGGC 3 cut(s) 65, 542, 2820
BcgI CGANNNNNNTGC 2 cut(s) 1616, 1650
BciT130I CCWGG 6 cut(s) 176, 1989, 2249, 2659, 2813, 3102
BciVI GTATCC 2 cut(s) 409, 839
BclI TGATCA 2 cut(s) 256, 2487
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 3 cut(s) 1031, 1337, 2151
BfaI CTAG 8 cut(s) 410, 738, 1359, 2082, 2744, 2891, 2930, 2955
BfmI CTRYAG 5 cut(s) 521, 1046, 2151, 2394, 2592
BfuAI ACCTGC 2 cut(s) 1039, 1504
BfuI GTATCC 2 cut(s) 409, 839
BisI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
BlnI CCTAGG 1 cut(s) 2890
BlsI GCNGC 5 cut(s) 214, 341, 2023, 2739, 2742
BmcAI AGTACT 2 cut(s) 408, 2392
Bme1390I CCNGG 7 cut(s) 104, 176, 1989, 2249, 2659, 2813, 3102
Bme18I GGWCC 5 cut(s) 987, 1975, 2126, 2277, 3098
BmgT120I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
BmiI GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
BmrFI CCNGG 7 cut(s) 104, 176, 1989, 2249, 2659, 2813, 3102
BmsI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
BmtI GCTAGC 1 cut(s) 2958
BpiI GAAGAC 1 cut(s) 1170
BpmI CTGGAG 2 cut(s) 255, 1512
BpuMI CCSGG 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 8 cut(s) 147, 558, 1988, 2174, 2220, 2728, 2863, 2890
BsaXI ACNNNNNCTCC 8 cut(s) 418, 448, 777, 807, 1791, 1821, 2584, 2614
Bse1I ACTGG 9 cut(s) 74, 248, 893, 1503, 2452, 2458, 2592, 2758, 3047
Bse3DI GCAATG 3 cut(s) 1165, 1215, 1296
BseBI CCWGG 6 cut(s) 176, 1989, 2249, 2659, 2813, 3102
BseDI CCNNGG 8 cut(s) 147, 558, 1988, 2174, 2220, 2728, 2863, 2890
BseGI GGATG 7 cut(s) 748, 1503, 1558, 1714, 1929, 2113, 2383
BseMI GCAATG 3 cut(s) 1165, 1215, 1296
BseMII CTCAG 6 cut(s) 646, 1007, 1265, 1347, 1530, 2960
BseNI ACTGG 9 cut(s) 74, 248, 893, 1503, 2452, 2458, 2592, 2758, 3047
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 1041
BseXI GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
BseYI CCCAGC 2 cut(s) 2024, 2880
Bsh1236I CGCG 2 cut(s) 144, 2948
BshFI GGCC 9 cut(s) 87, 107, 287, 598, 2192, 2408, 2626, 2662, 3105
BsiHKAI GWGCWC 2 cut(s) 1498, 1707
BsiSI CCGG 2 cut(s) 53, 103
BslFI GGGAC 4 cut(s) 110, 591, 2290, 2430
BsmAI GTCTC 3 cut(s) 1031, 1337, 2151
BsmFI GGGAC 4 cut(s) 110, 591, 2290, 2430
BsmI GAATGC 2 cut(s) 306, 2798
BsnI GGCC 9 cut(s) 87, 107, 287, 598, 2192, 2408, 2626, 2662, 3105
Bso31I GGTCTC 1 cut(s) 1031
Bsp1286I GDGCHC 2 cut(s) 1498, 1707
Bsp1407I TGTACA 1 cut(s) 2427
Bsp143I GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
BspACI CCGC 2 cut(s) 46, 469
BspANI GGCC 9 cut(s) 87, 107, 287, 598, 2192, 2408, 2626, 2662, 3105
BspCNI CTCAG 6 cut(s) 645, 1008, 1266, 1346, 1531, 2961
BspFNI CGCG 2 cut(s) 144, 2948
BspLI GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
BspMAI CTGCAG 2 cut(s) 1050, 2155
BspMI ACCTGC 2 cut(s) 1039, 1504
BspOI GCTAGC 1 cut(s) 2958
BspPI GGATC 2 cut(s) 125, 2190
BspTNI GGTCTC 1 cut(s) 1031
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 3 cut(s) 1165, 1215, 1296
BsrGI TGTACA 1 cut(s) 2427
BsrI ACTGG 9 cut(s) 74, 248, 893, 1503, 2452, 2458, 2592, 2758, 3047
BssECI CCNNGG 8 cut(s) 147, 558, 1988, 2174, 2220, 2728, 2863, 2890
BssHII GCGCGC 1 cut(s) 142
BssMI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
BssNAI GTATAC 1 cut(s) 437
BssT1I CCWWGG 2 cut(s) 2220, 2890
Bst1107I GTATAC 1 cut(s) 437
Bst2UI CCWGG 6 cut(s) 176, 1989, 2249, 2659, 2813, 3102
Bst6I CTCTTC 2 cut(s) 276, 1918
BstAPI GCANNNNNTGC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 2427
BstC8I GCNNGC 6 cut(s) 50, 144, 2335, 2956, 2975, 3017
BstDEI CTNAG 7 cut(s) 632, 1016, 1154, 1274, 1333, 1539, 2969
BstDSI CCRYGG 3 cut(s) 558, 2728, 2863
BstEII GGTNACC 1 cut(s) 809
BstENI CCTNNNNNAGG 2 cut(s) 1019, 1511
BstF5I GGATG 7 cut(s) 748, 1503, 1558, 1714, 1929, 2113, 2383
BstFNI CGCG 2 cut(s) 144, 2948
BstHHI GCGC 4 cut(s) 144, 146, 1644, 2948
BstKTI GATC 7 cut(s) 133, 259, 494, 1167, 1813, 2198, 2490
BstMAI GTCTC 3 cut(s) 1031, 1337, 2151
BstMBI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
BstMWI GCNNNNNNNGC 7 cut(s) 49, 468, 685, 1054, 1868, 2334, 2913
BstNI CCWGG 6 cut(s) 176, 1989, 2249, 2659, 2813, 3102
BstNSI RCATGY 5 cut(s) 125, 610, 2146, 2508, 3019
BstPI GGTNACC 1 cut(s) 809
BstSCI CCNGG 7 cut(s) 102, 174, 1987, 2247, 2657, 2811, 3100
BstSFI CTRYAG 5 cut(s) 521, 1046, 2151, 2394, 2592
BstUI CGCG 2 cut(s) 144, 2948
BstV1I GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
BstV2I GAAGAC 1 cut(s) 1170
BstXI CCANNNNNNTGG 1 cut(s) 2973
BstZ17I GTATAC 1 cut(s) 437
BsuI GTATCC 2 cut(s) 409, 839
BsuRI GGCC 9 cut(s) 87, 107, 287, 598, 2192, 2408, 2626, 2662, 3105
BtgI CCRYGG 3 cut(s) 558, 2728, 2863
BtgZI GCGATG 1 cut(s) 1693
BtsCI GGATG 7 cut(s) 748, 1503, 1558, 1714, 1929, 2113, 2383
BtsI GCAGTG 3 cut(s) 657, 810, 1043
BveI ACCTGC 2 cut(s) 1039, 1504
Cac8I GCNNGC 6 cut(s) 50, 144, 2335, 2956, 2975, 3017
CaiI CAGNNNCTG 2 cut(s) 254, 1247
CfoI GCGC 4 cut(s) 144, 146, 1644, 2948
Cfr13I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
CseI GACGC 2 cut(s) 1973, 2294
Csp6I GTAC 5 cut(s) 407, 2391, 2428, 2643, 2963
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviQI GTAC 5 cut(s) 407, 2391, 2428, 2643, 2963
DdeI CTNAG 7 cut(s) 632, 1016, 1154, 1274, 1333, 1539, 2969
DpnI GATC 7 cut(s) 132, 258, 493, 1166, 1812, 2197, 2489
DpnII GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
DraI TTTAAA 1 cut(s) 1897
EaeI YGGCCR 2 cut(s) 596, 2406
Eam1104I CTCTTC 2 cut(s) 276, 1918
EarI CTCTTC 2 cut(s) 276, 1918
Eco130I CCWWGG 2 cut(s) 2220, 2890
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 5 cut(s) 987, 1975, 2126, 2277, 3098
Eco57I CTGAAG 3 cut(s) 300, 1269, 1887
Eco91I GGTNACC 1 cut(s) 809
EcoNI CCTNNNNNAGG 2 cut(s) 1019, 1511
EcoO109I RGGNCCY 2 cut(s) 1975, 2625
EcoO65I GGTNACC 1 cut(s) 809
EcoRII CCWGG 6 cut(s) 174, 1987, 2247, 2657, 2811, 3100
EcoT14I CCWWGG 2 cut(s) 2220, 2890
EcoT22I ATGCAT 2 cut(s) 2800, 3044
ErhI CCWWGG 2 cut(s) 2220, 2890
FalI AAGNNNNNCTT 2 cut(s) 2204, 2236
FaqI GGGAC 4 cut(s) 110, 591, 2290, 2430
FauNDI CATATG 2 cut(s) 1367, 1404
FbaI TGATCA 2 cut(s) 256, 2487
FblI GTMKAC 1 cut(s) 436
Fnu4HI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
FokI GGATG 7 cut(s) 755, 1490, 1565, 1701, 1916, 2120, 2390
Fsp4HI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
FspBI CTAG 8 cut(s) 410, 738, 1359, 2082, 2744, 2891, 2930, 2955
FspI TGCGCA 1 cut(s) 1643
GlaI GCGC 4 cut(s) 143, 145, 1643, 2947
GluI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
GsaI CCCAGC 2 cut(s) 2028, 2884
GsuI CTGGAG 2 cut(s) 255, 1512
HaeIII GGCC 9 cut(s) 87, 107, 287, 598, 2192, 2408, 2626, 2662, 3105
HapII CCGG 2 cut(s) 53, 103
HgaI GACGC 2 cut(s) 1973, 2294
HhaI GCGC 4 cut(s) 144, 146, 1644, 2948
Hin6I GCGC 4 cut(s) 142, 144, 1642, 2946
HinP1I GCGC 4 cut(s) 142, 144, 1642, 2946
HincII GTYRAC 4 cut(s) 245, 2463, 2470, 2554
HindII GTYRAC 4 cut(s) 245, 2463, 2470, 2554
HindIII AAGCTT 2 cut(s) 871, 2647
HinfI GANTC 8 cut(s) 866, 1276, 1589, 1802, 1910, 1968, 2179, 2748
HpaI GTTAAC 2 cut(s) 2463, 2470
HpaII CCGG 2 cut(s) 53, 103
Hpy166II GTNNAC 7 cut(s) 245, 437, 1256, 2428, 2463, 2470, 2554
Hpy8I GTNNAC 7 cut(s) 245, 437, 1256, 2428, 2463, 2470, 2554
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 8 cut(s) 105, 160, 410, 596, 1244, 1990, 2117, 2603
HpyCH4IV ACGT 3 cut(s) 1470, 2419, 2472
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 468, 685, 1054, 1868, 2334, 2913
HpyF3I CTNAG 7 cut(s) 632, 1016, 1154, 1274, 1333, 1539, 2969
HpySE526I ACGT 3 cut(s) 1470, 2419, 2472
HspAI GCGC 4 cut(s) 142, 144, 1642, 2946
Ksp22I TGATCA 2 cut(s) 256, 2487
KspAI GTTAAC 2 cut(s) 2463, 2470
Kzo9I GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
LmnI GCTCC 3 cut(s) 1493, 2293, 2527
Lsp1109I GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
LweI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
MaeI CTAG 8 cut(s) 410, 738, 1359, 2082, 2744, 2891, 2930, 2955
MaeII ACGT 3 cut(s) 1470, 2419, 2472
MalI GATC 7 cut(s) 132, 258, 493, 1166, 1812, 2197, 2489
MbiI CCGCTC 1 cut(s) 48
MboI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
MfeI CAATTG 2 cut(s) 567, 2699
MhlI GDGCHC 2 cut(s) 1498, 1707
MlsI TGGCCA 2 cut(s) 598, 2408
MluNI TGGCCA 2 cut(s) 598, 2408
MlyI GAGTC 5 cut(s) 875, 1285, 1598, 1904, 2742
MmeI TCCRAC 1 cut(s) 142
Mox20I TGGCCA 2 cut(s) 598, 2408
Mph1103I ATGCAT 2 cut(s) 2800, 3044
MroXI GAANNNNTTC 2 cut(s) 183, 2243
MscI TGGCCA 2 cut(s) 598, 2408
MslI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
Msp20I TGGCCA 2 cut(s) 598, 2408
MspA1I CMGCKG 2 cut(s) 2740, 2880
MspI CCGG 2 cut(s) 53, 103
MspR9I CCNGG 7 cut(s) 104, 176, 1989, 2249, 2659, 2813, 3102
MunI CAATTG 2 cut(s) 567, 2699
Mva1269I GAATGC 2 cut(s) 306, 2798
MvaI CCWGG 6 cut(s) 176, 1989, 2249, 2659, 2813, 3102
MvnI CGCG 2 cut(s) 144, 2948
MwoI GCNNNNNNNGC 7 cut(s) 49, 468, 685, 1054, 1868, 2334, 2913
NciI CCSGG 1 cut(s) 104
NdeI CATATG 2 cut(s) 1367, 1404
NdeII GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
NheI GCTAGC 1 cut(s) 2954
NlaIV GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
NsbI TGCGCA 1 cut(s) 1643
NsiI ATGCAT 2 cut(s) 2800, 3044
NspI RCATGY 5 cut(s) 125, 610, 2146, 2508, 3019
OliI CACNNNNGTG 1 cut(s) 2222
PaeI GCATGC 1 cut(s) 3019
PaqCI CACCTGC 1 cut(s) 1039
PauI GCGCGC 1 cut(s) 142
PciI ACATGT 2 cut(s) 121, 2142
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 2 cut(s) 306, 2798
PdmI GAANNNNTTC 2 cut(s) 183, 2243
PfeI GAWTC 3 cut(s) 1802, 1968, 2179
PflMI CCANNNNNTGG 2 cut(s) 827, 1422
PfoI TCCNGGA 2 cut(s) 174, 2247
PkrI GCNGC 5 cut(s) 214, 341, 2023, 2739, 2742
PleI GAGTC 5 cut(s) 874, 1284, 1597, 1904, 2742
PpsI GAGTC 5 cut(s) 874, 1284, 1597, 1904, 2742
PpuMI RGGWCCY 1 cut(s) 1975
PscI ACATGT 2 cut(s) 121, 2142
PshBI ATTAAT 1 cut(s) 1301
Psp1406I AACGTT 1 cut(s) 2472
Psp5II RGGWCCY 1 cut(s) 1975
Psp6I CCWGG 6 cut(s) 174, 1987, 2247, 2657, 2811, 3100
PspEI GGTNACC 1 cut(s) 809
PspFI CCCAGC 2 cut(s) 2024, 2880
PspGI CCWGG 6 cut(s) 174, 1987, 2247, 2657, 2811, 3100
PspN4I GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
PspPI GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
PspPPI RGGWCCY 1 cut(s) 1975
PstI CTGCAG 2 cut(s) 1050, 2155
PstNI CAGNNNCTG 2 cut(s) 254, 1247
PteI GCGCGC 1 cut(s) 142
PvuII CAGCTG 2 cut(s) 2740, 2880
RsaI GTAC 5 cut(s) 408, 2392, 2429, 2644, 2964
RsaNI GTAC 5 cut(s) 407, 2391, 2428, 2643, 2963
RseI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
SatI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
Sau3AI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
Sau96I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
ScaI AGTACT 2 cut(s) 408, 2392
SchI GAGTC 5 cut(s) 875, 1285, 1598, 1904, 2742
ScrFI CCNGG 7 cut(s) 104, 176, 1989, 2249, 2659, 2813, 3102
SduI GDGCHC 2 cut(s) 1498, 1707
SfaNI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
SfcI CTRYAG 5 cut(s) 521, 1046, 2151, 2394, 2592
SinI GGWCC 5 cut(s) 987, 1975, 2126, 2277, 3098
SmiMI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
SphI GCATGC 1 cut(s) 3019
SsiI CCGC 2 cut(s) 46, 469
SspMI CTAG 8 cut(s) 410, 738, 1359, 2082, 2744, 2891, 2930, 2955
StyD4I CCNGG 7 cut(s) 102, 174, 1987, 2247, 2657, 2811, 3100
StyI CCWWGG 2 cut(s) 2220, 2890
TaiI ACGT 3 cut(s) 1473, 2422, 2475
TaqI TCGA 2 cut(s) 187, 2721
TaqII GACCGA 1 cut(s) 1004
TatI WGTACW 3 cut(s) 406, 2390, 2427
TfiI GAWTC 3 cut(s) 1802, 1968, 2179
TseFI GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
TseI GCWGC 5 cut(s) 212, 339, 2021, 2737, 2740
Tsp45I GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
TspGWI ACGGA 5 cut(s) 391, 491, 1388, 2717, 2852
Van91I CCANNNNNTGG 2 cut(s) 827, 1422
VpaK11BI GGWCC 5 cut(s) 987, 1975, 2126, 2277, 3098
VspI ATTAAT 1 cut(s) 1301
XagI CCTNNNNNAGG 2 cut(s) 1019, 1511
XapI RAATTY 7 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2230
XceI RCATGY 5 cut(s) 125, 610, 2146, 2508, 3019
XcmI CCANNNNNNNNNTGG 1 cut(s) 1402
XmaJI CCTAGG 1 cut(s) 2890
XmiI GTMKAC 1 cut(s) 436
XmnI GAANNNNTTC 2 cut(s) 183, 2243
XspI CTAG 8 cut(s) 410, 738, 1359, 2082, 2744, 2891, 2930, 2955
ZrmI AGTACT 2 cut(s) 408, 2392
Zsp2I ATGCAT 2 cut(s) 2800, 3044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.