MD14G1077000.v1.1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002172
Physical Location & Seq
Forward (+)
8718181 .. 8726517
8337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1077000.v1.1.491

Sequence Viewer

Length: 3120 bp
ATGGAGAAGTACTTGAAGGACTTCGAGGTGGAGAACAAGAATCCGTCGGAGGAGACTATAAGGAGATGGAGAAACGCCGTCGCGCTCGTCAAAAACCCTCGCAGAAGGTTCCGATTCGTCGCCGATCTCGCCAAACGATCTGAAGCCGAGAAGAAGAAGCTTCAAATCCAGGAAAAGATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTGCAGTTCATTGATGCTGTTGATCGTGGAGCTGATGCAAAGCCAGGTCTGCATGAATTCAAGCTGTCAGAAGATGCCAGAATGGCAGGTTTCAGCATTCACCCAGATGAACTTGCATCTATTACACGTGCCCATGATATTAAGGCCTTGAAAAGCCATGGTGGAATTCATGGGATTTTAAGGAAAGTCTCTGTCTCGGTAGATGAAGGTGTCAAAGATAGTAATATAGCAATCAGGCAAAATGTTTATGGCTTAAACCGTTACAAGGAGAAACCTCCTCGAACTTTTTTGGTGTTTGTATGGGAAGCACTACAGGACTTAACATTAATGATCCTTATGGTCTGTGCTGTGGTTTCAATTGGAGTTGGAATTGCCACTGAAGGCTGGCCCAAAGGCACGTACGATGGTCTGGGAATTTTAATTAGTATAATTTTAGTGGTTACAGTTACCGCCATTAGTGACTACAAGCAGTCTTTGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTCTGTTCAGGTCACTAGGGATGGAAAAAGACAAAAAGTTTCCATTCACGACTTGGTTGTTGGAGATATTGTGCATTTGTCGGTTGGGGATGTAGTTCCAGCTGATGGACTTTTCATATCAGGGTACAGTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCGGTGAATATATATGAAGAGAAGCCTTTTCTTCTTTCCGGAACTACAGTGCAGGATGGATCAGGTAAAATGCTAGTGACTGCAGTTGGTATGAGGACTGAATGGGGAAAATTGATGGAAACACTGAGCGAAGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTTAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGACTTTTGCAGTGTTGACATTTTTGGTATTGACGGTAAGATTTTTGGTGACAAAAGGACTTAACAACGAGATCACTGATTGGTCTTCAACTGACGCCGTAACACTTTTGAACTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACCATTGGCAGTGACGCTGAGTCTTGCTTTTGCAATGAAAAAATTGATGAATGATAGGGCACTTGTAAGGCATCTCTCAGCATGTGAGACAATGGGTTCTGCTGGTTGTATTTGCACAGATAAAACAGGAACATTAACTACAAATCATATGGTAGTTACCAAAGTATGGATATGTGAAAAATCTGTAGATGTTAAAGAAAATGACAGTAAAGAAACATTGACATCAGAAATATCTGGAGCATCAAGCATCCTTTTGCAGGTTATATTTCAAAATACAAGTTCTGAGGTTATTAAGGATGATGGAAAGACCTCTATTTTGGGAACACCAACGGAGTCAGCATTGCTAGAGTTTGGTTTACTTTTAGGTGGCGATTTTGATGCCCTGCGCAGAGAGGTAAGGATTCTTAAGATTGAACCTTTTAATTCTGTCAGGAAGAAAATGTCTGTTCTTGTAGCTCATCCTCATGGTGGAAAACGAGCGTTTTGCAAAGGTGCATCAGAACTAGTACTGGGAATATGTAACAAGTACATTGACTCTAATGGAGAGCCTGTTCATCTCTCCGAAGAAATGGTAAAGAATATCACGAATGTCATAAATACTTTTGCCTGTGAAGCTTTGAGAACTCTCTGCTTAGCTTTCAAGGATATAGATGACTCTTCCATCGAAAGTGGCATCCCAGATGACGGCTATACATTGGTAGCAGTTGTTGGTATTAAGGATCCTGTGCGCCCGGGGGTCAAGGAGGCAGTTGAGACTTGTTTAGCTGCTGGAATCACTGTACGTATGGTAACTGGTGATAATATAAATACAGCTAAAGCCATTGCTAAAGAATGTGGCATACTCACAGAGGGTGGTATAGCCATAGAAGGACCTGCGTTTCGTAGCATGTCTCTTGAGCAGATGAAAACTGTGATACCGAAGATTCAGGTAATGGCCCGGTCTTTACCTTTGGATAAGCACACATTGGTAAAAACTTTGAGGGATGAATTTGGTGAGGTAGTTGCAGTGACTGGTGATGGGACTAATGACGCTCCTGCTTTGAAAGAGTCAGACATTGGACTTGCTATGGGCATAGCAGGAACAGAGGTTGCCAAAGAAAGTGCCGATGTCATCATATTGGATGACAATTTTAAAACTATAGTAAATGTGGCCAGATGGGGACGTTCAGTATACATAAACATTCAGAAGTTTGTGCAGTTCCAGTTAACTGTTAACGTTGTTGCTCTAATAATCAATTTTGTTTCTGCATGTGTCTCAGGATCTACTCCCCTTACAGCAGTGCAACTGCTTTGGGTGAACATGATTATGGACACTCTTGGTGCTTTGGCACTGGCAACAGAGCCTCCAAATGATGGACTTATGAAAAGGCCCCCAGTTGGGAGGGGTACAAGCTTCATCACCAAGGCTATGTGGAGGAATATCATTGGTCAGAGTATCTATCAACTGGCTGTCCTTGGAGTTCTTGATTTCTCTGGGAAGAAGCTACTAGGACTTACTGGTTCAGATGCAACTGAGGTTCTTAACACTGTGATATTCAACGCATTTGTGTTTTGCCAGGTGTTTAATCAGATAAACAGTCGTGACATAGAGAGGATAAACATATTCCGTGGAATGTTTGATAGCTGGATATTCCTAGGCGTCATGGTTTGCACAGTGGTCTTCCAGGCTATCATAGTAGAGTTCTTGGGAGATTTTGCTAGCACTGTACCACTAAGCTGGCAACTATGGGTACTCAGCGTTTTACTTGGATCTGTTAGCATGCTTGTTGCAGTTGTGCTCAAATTAATACCTGTCGAAAGAACAATTAAGCACCATGACGGATATGAAGCACTTCCTTCTGGTCCTCCGGAGGGCATAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1040

Amino Acids

113.23

Weight (kDa)

6.04

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 6 - 48 7.7e-19 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 122 - 189 3.4e-13 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 240 - 338 7.8e-24 P-type ATPase actuator domain
Cation_ATPase PF13246 513 - 663 7.4e-31 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 633 - 769 3e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1015 2e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1039
Acc16I TGCGCA 1 cut(s) 1643
Acc36I ACCTGC 4 cut(s) 287, 1039, 1504, 2137
AccB7I CCANNNNNTGG 3 cut(s) 827, 1422, 2609
AccI GTMKAC 1 cut(s) 2427
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 2 cut(s) 929, 3103
AciI CCGC 2 cut(s) 660, 1220
AclI AACGTT 1 cut(s) 2472
AclWI GGATC 6 cut(s) 535, 958, 1970, 1983, 2524, 3013
AcoI YGGCCR 1 cut(s) 2406
AcsI RAATTY 4 cut(s) 266, 375, 624, 2243
AcuI CTGAAG 2 cut(s) 162, 609
AcvI CACGTG 1 cut(s) 338
AcyI GRCGYC 2 cut(s) 1188, 2895
AfaI GTAC 9 cut(s) 11, 611, 848, 1764, 1784, 2037, 2644, 2964, 2988
AflII CTTAAG 1 cut(s) 1661
AflIII ACRYGT 1 cut(s) 335
AhdI GACNNNNNGTC 1 cut(s) 1275
AhlI ACTAGT 1 cut(s) 1759
AjnI CCWGG 4 cut(s) 168, 253, 2811, 2919
AloI GAACNNNNNNTCC 2 cut(s) 1791, 1823
Alw21I GWGCWC 1 cut(s) 3036
Alw26I GTCTC 8 cut(s) 47, 403, 409, 1031, 1337, 2003, 2151, 2515
AlwI GGATC 6 cut(s) 535, 958, 1970, 1983, 2524, 3013
AlwNI CAGNNNCTG 1 cut(s) 2267
Ama87I CYCGRG 1 cut(s) 1987
Aor13HI TCCGGA 2 cut(s) 929, 3103
AoxI GGCC 5 cut(s) 354, 596, 2190, 2406, 2624
ApeKI GCWGC 2 cut(s) 206, 2021
ApoI RAATTY 4 cut(s) 266, 375, 624, 2243
ArsI GACNNNNNNTTYG 4 cut(s) 1555, 1587, 2118, 2150
AseI ATTAAT 2 cut(s) 536, 3041
Asp700I GAANNNNTTC 3 cut(s) 20, 177, 3087
AspA2I CCTAGG 1 cut(s) 2890
AspLEI GCGC 3 cut(s) 85, 1644, 1986
AspS9I GGNCC 5 cut(s) 597, 2126, 2191, 2625, 3098
AsuC2I CCSGG 3 cut(s) 1988, 1989, 2194
AsuNHI GCTAGC 1 cut(s) 2954
AvaI CYCGRG 1 cut(s) 1987
AvaII GGWCC 2 cut(s) 2126, 3098
AvrII CCTAGG 1 cut(s) 2890
BaeGI GKGCMC 2 cut(s) 343, 1318
BalI TGGCCA 1 cut(s) 2408
BamHI GGATCC 1 cut(s) 1975
BbrPI CACGTG 1 cut(s) 338
BbsI GAAGAC 2 cut(s) 1170, 2908
Bbv12I GWGCWC 1 cut(s) 3036
BbvI GCAGC 2 cut(s) 218, 2008
BceAI ACGGC 3 cut(s) 62, 1175, 1957
BcgI CGANNNNNNTGC 4 cut(s) 1616, 1650, 1722, 1756
BciT130I CCWGG 4 cut(s) 170, 255, 2813, 2921
BcnI CCSGG 3 cut(s) 1988, 1989, 2194
BcoDI GTCTC 8 cut(s) 47, 403, 409, 1031, 1337, 2003, 2151, 2515
BcuI ACTAGT 1 cut(s) 1759
BfaI CTAG 7 cut(s) 738, 965, 1601, 1760, 2744, 2891, 2955
BfmI CTRYAG 7 cut(s) 212, 521, 936, 972, 1046, 1440, 2394
BfrI CTTAAG 1 cut(s) 1661
BfuAI ACCTGC 4 cut(s) 287, 1039, 1504, 2137
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 2 cut(s) 207, 2022
BlnI CCTAGG 1 cut(s) 2890
BlpI GCTNAGC 1 cut(s) 1888
BlsI GCNGC 2 cut(s) 208, 2023
BmcAI AGTACT 2 cut(s) 11, 1764
Bme1390I CCNGG 7 cut(s) 170, 255, 1988, 1989, 2194, 2813, 2921
Bme18I GGWCC 2 cut(s) 2126, 3098
BmeRI GACNNNNNGTC 1 cut(s) 1275
BmeT110I CYCGRG 1 cut(s) 1987
BmgT120I GGNCC 5 cut(s) 597, 2126, 2191, 2625, 3098
BmiI GGNNCC 3 cut(s) 110, 1977, 2627
BmrFI CCNGG 7 cut(s) 170, 255, 1988, 1989, 2194, 2813, 2921
BmrI ACTGGG 2 cut(s) 1775, 2624
BmtI GCTAGC 1 cut(s) 2958
BmuI ACTGGG 2 cut(s) 1775, 2624
BpiI GAAGAC 2 cut(s) 1170, 2908
BpmI CTGGAG 1 cut(s) 1512
Bpu1102I GCTNAGC 1 cut(s) 1888
BpuEI CTTGAG 1 cut(s) 2171
BpuMI CCSGG 3 cut(s) 1988, 1989, 2194
BsaAI YACGTR 3 cut(s) 338, 609, 2039
BsaHI GRCGYC 2 cut(s) 1188, 2895
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 7 cut(s) 367, 1987, 1988, 2658, 2710, 2863, 2890
BsaWI WCCGGW 2 cut(s) 929, 3103
BsaXI ACNNNNNCTCC 6 cut(s) 777, 807, 1791, 1821, 2584, 2614
Bse118I RCCGGY 1 cut(s) 892
Bse1I ACTGG 8 cut(s) 1770, 2053, 2272, 2458, 2592, 2630, 2706, 2758
Bse3DI GCAATG 3 cut(s) 1296, 1595, 2076
BseAI TCCGGA 2 cut(s) 929, 3103
BseBI CCWGG 4 cut(s) 170, 255, 2813, 2921
BseDI CCNNGG 7 cut(s) 367, 1987, 1988, 2658, 2710, 2863, 2890
BseGI GGATG 9 cut(s) 748, 817, 952, 1503, 1558, 1714, 1929, 2245, 2383
BseMI GCAATG 3 cut(s) 1296, 1595, 2076
BseMII CTCAG 7 cut(s) 1007, 1265, 1347, 1530, 2526, 2760, 3004
BseNI ACTGG 8 cut(s) 1770, 2053, 2272, 2458, 2592, 2630, 2706, 2758
BseRI GAGGAG 3 cut(s) 65, 477, 1041
BseSI GKGCMC 2 cut(s) 343, 1318
BseXI GCAGC 2 cut(s) 218, 2008
BsgI GTGCAG 2 cut(s) 962, 2471
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 5 cut(s) 356, 598, 2192, 2408, 2626
BsiHKAI GWGCWC 1 cut(s) 3036
BsiHKCI CYCGRG 1 cut(s) 1987
BsiSI CCGG 5 cut(s) 893, 930, 1988, 2194, 3104
BsiWI CGTACG 1 cut(s) 609
BslFI GGGAC 2 cut(s) 2290, 2430
BsmAI GTCTC 8 cut(s) 47, 403, 409, 1031, 1337, 2003, 2151, 2515
BsmFI GGGAC 2 cut(s) 2290, 2430
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 5 cut(s) 356, 598, 2192, 2408, 2626
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 1 cut(s) 1987
Bsp1286I GDGCHC 3 cut(s) 343, 1318, 3036
Bsp13I TCCGGA 2 cut(s) 929, 3103
Bsp143I GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
Bsp1720I GCTNAGC 1 cut(s) 1888
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 2 cut(s) 660, 1220
BspANI GGCC 5 cut(s) 356, 598, 2192, 2408, 2626
BspCNI CTCAG 7 cut(s) 1008, 1266, 1346, 1531, 2525, 2761, 3003
BspEI TCCGGA 2 cut(s) 929, 3103
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 3 cut(s) 110, 1977, 2627
BspMAI CTGCAG 3 cut(s) 216, 976, 1050
BspMI ACCTGC 4 cut(s) 287, 1039, 1504, 2137
BspOI GCTAGC 1 cut(s) 2958
BspPI GGATC 6 cut(s) 535, 958, 1970, 1983, 2524, 3013
BspTI CTTAAG 1 cut(s) 1661
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 3 cut(s) 1296, 1595, 2076
BsrFI RCCGGY 1 cut(s) 892
BsrI ACTGG 8 cut(s) 1770, 2053, 2272, 2458, 2592, 2630, 2706, 2758
BssAI RCCGGY 1 cut(s) 892
BssECI CCNNGG 7 cut(s) 367, 1987, 1988, 2658, 2710, 2863, 2890
BssMI GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
BssNAI GTATAC 1 cut(s) 2428
BssNI GRCGYC 2 cut(s) 1188, 2895
BssT1I CCWWGG 4 cut(s) 367, 2658, 2710, 2890
Bst1107I GTATAC 1 cut(s) 2428
Bst2UI CCWGG 4 cut(s) 170, 255, 2813, 2921
Bst6I CTCTTC 2 cut(s) 903, 1918
BstACI GRCGYC 2 cut(s) 1188, 2895
BstAFI CTTAAG 1 cut(s) 1661
BstAPI GCANNNNNTGC 1 cut(s) 685
BstBAI YACGTR 3 cut(s) 338, 609, 2039
BstC8I GCNNGC 4 cut(s) 596, 2956, 2975, 3017
BstDEI CTNAG 9 cut(s) 1016, 1274, 1333, 1539, 1888, 2512, 2769, 2969, 2990
BstDSI CCRYGG 2 cut(s) 367, 2863
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 9 cut(s) 748, 817, 952, 1503, 1558, 1714, 1929, 2245, 2383
BstFNI CGCG 1 cut(s) 83
BstHHI GCGC 3 cut(s) 85, 1644, 1986
BstKTI GATC 9 cut(s) 127, 140, 235, 543, 953, 1167, 1978, 2519, 3008
BstMAI GTCTC 8 cut(s) 47, 403, 409, 1031, 1337, 2003, 2151, 2515
BstMBI GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
BstMWI GCNNNNNNNGC 6 cut(s) 128, 259, 293, 685, 1054, 1868
BstNI CCWGG 4 cut(s) 170, 255, 2813, 2921
BstNSI RCATGY 4 cut(s) 1341, 2146, 2508, 3019
BstSCI CCNGG 7 cut(s) 168, 253, 1986, 1987, 2192, 2811, 2919
BstSFI CTRYAG 7 cut(s) 212, 521, 936, 972, 1046, 1440, 2394
BstSLI GKGCMC 2 cut(s) 343, 1318
BstSNI TACGTA 1 cut(s) 2039
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 218, 2008
BstV2I GAAGAC 2 cut(s) 1170, 2908
BstX2I RGATCY 3 cut(s) 1975, 2516, 3005
BstXI CCANNNNNNTGG 1 cut(s) 2973
BstYI RGATCY 3 cut(s) 1975, 2516, 3005
BstZ17I GTATAC 1 cut(s) 2428
BsuRI GGCC 5 cut(s) 356, 598, 2192, 2408, 2626
BtgI CCRYGG 2 cut(s) 367, 2863
BtsCI GGATG 9 cut(s) 748, 817, 952, 1503, 1558, 1714, 1929, 2245, 2383
BtsI GCAGTG 6 cut(s) 209, 1043, 1110, 1272, 2268, 2541
BveI ACCTGC 4 cut(s) 287, 1039, 1504, 2137
Cac8I GCNNGC 4 cut(s) 596, 2956, 2975, 3017
CaiI CAGNNNCTG 1 cut(s) 2267
CfoI GCGC 3 cut(s) 85, 1644, 1986
Cfr10I RCCGGY 1 cut(s) 892
Cfr13I GGNCC 5 cut(s) 597, 2126, 2191, 2625, 3098
Cfr9I CCCGGG 1 cut(s) 1987
CseI GACGC 4 cut(s) 1196, 1279, 2294, 2884
Csp6I GTAC 9 cut(s) 10, 610, 847, 1763, 1783, 2036, 2643, 2963, 2987
CviQI GTAC 9 cut(s) 10, 610, 847, 1763, 1783, 2036, 2643, 2963, 2987
DdeI CTNAG 9 cut(s) 1016, 1274, 1333, 1539, 1888, 2512, 2769, 2969, 2990
DpnI GATC 9 cut(s) 126, 139, 234, 542, 952, 1166, 1977, 2518, 3007
DpnII GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
DraI TTTAAA 1 cut(s) 2389
DriI GACNNNNNGTC 1 cut(s) 1275
EaeI YGGCCR 1 cut(s) 2406
Eam1104I CTCTTC 2 cut(s) 903, 1918
Eam1105I GACNNNNNGTC 1 cut(s) 1275
EarI CTCTTC 2 cut(s) 903, 1918
Eco105I TACGTA 1 cut(s) 2039
Eco130I CCWWGG 4 cut(s) 367, 2658, 2710, 2890
Eco147I AGGCCT 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 2 cut(s) 2126, 3098
Eco57I CTGAAG 2 cut(s) 162, 609
Eco72I CACGTG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 1987
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 2 cut(s) 2126, 2625
EcoRI GAATTC 2 cut(s) 266, 375
EcoRII CCWGG 4 cut(s) 168, 253, 2811, 2919
EcoT14I CCWWGG 4 cut(s) 367, 2658, 2710, 2890
ErhI CCWWGG 4 cut(s) 367, 2658, 2710, 2890
FaqI GGGAC 2 cut(s) 2290, 2430
FauNDI CATATG 1 cut(s) 1404
FblI GTMKAC 1 cut(s) 2427
Fnu4HI GCNGC 2 cut(s) 207, 2022
FokI GGATG 9 cut(s) 755, 824, 959, 1490, 1565, 1701, 1916, 2252, 2390
Fsp4HI GCNGC 2 cut(s) 207, 2022
FspBI CTAG 7 cut(s) 738, 965, 1601, 1760, 2744, 2891, 2955
FspI TGCGCA 1 cut(s) 1643
GlaI GCGC 3 cut(s) 84, 1643, 1985
GluI GCNGC 2 cut(s) 207, 2022
GsuI CTGGAG 1 cut(s) 1512
HaeIII GGCC 5 cut(s) 356, 598, 2192, 2408, 2626
HapII CCGG 5 cut(s) 893, 930, 1988, 2194, 3104
HgaI GACGC 4 cut(s) 1196, 1279, 2294, 2884
HhaI GCGC 3 cut(s) 85, 1644, 1986
Hin1I GRCGYC 2 cut(s) 1188, 2895
Hin6I GCGC 3 cut(s) 83, 1642, 1984
HinP1I GCGC 3 cut(s) 83, 1642, 1984
HincII GTYRAC 3 cut(s) 1110, 2463, 2470
HindII GTYRAC 3 cut(s) 1110, 2463, 2470
HindIII AAGCTT 5 cut(s) 158, 871, 1055, 1869, 2647
HpaI GTTAAC 2 cut(s) 2463, 2470
HpaII CCGG 5 cut(s) 893, 930, 1988, 2194, 3104
Hpy166II GTNNAC 6 cut(s) 1110, 1613, 2428, 2463, 2470, 2554
Hpy8I GTNNAC 6 cut(s) 1110, 1613, 2428, 2463, 2470, 2554
Hpy99I CGWCG 3 cut(s) 49, 83, 122
HpyAV CCTTC 8 cut(s) 10, 99, 410, 584, 1016, 1244, 2117, 3102
HpyCH4IV ACGT 5 cut(s) 337, 608, 2038, 2419, 2472
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 259, 293, 685, 1054, 1868
HpyF3I CTNAG 9 cut(s) 1016, 1274, 1333, 1539, 1888, 2512, 2769, 2969, 2990
HpySE526I ACGT 5 cut(s) 337, 608, 2038, 2419, 2472
Hsp92I GRCGYC 2 cut(s) 1188, 2895
HspAI GCGC 3 cut(s) 83, 1642, 1984
Kpn2I TCCGGA 2 cut(s) 929, 3103
KspAI GTTAAC 2 cut(s) 2463, 2470
Kzo9I GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
LmnI GCTCC 3 cut(s) 239, 1493, 2293
Lsp1109I GCAGC 2 cut(s) 218, 2008
MaeI CTAG 7 cut(s) 738, 965, 1601, 1760, 2744, 2891, 2955
MaeII ACGT 5 cut(s) 337, 608, 2038, 2419, 2472
MalI GATC 9 cut(s) 126, 139, 234, 542, 952, 1166, 1977, 2518, 3007
MboI GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 3 cut(s) 1975, 2516, 3005
MhlI GDGCHC 3 cut(s) 343, 1318, 3036
MlsI TGGCCA 1 cut(s) 2408
MluNI TGGCCA 1 cut(s) 2408
MlyI GAGTC 6 cut(s) 875, 1285, 1598, 1784, 1904, 2312
MmeI TCCRAC 3 cut(s) 27, 556, 763
Mox20I TGGCCA 1 cut(s) 2408
MroI TCCGGA 2 cut(s) 929, 3103
MroXI GAANNNNTTC 3 cut(s) 20, 177, 3087
MscI TGGCCA 1 cut(s) 2408
MslI CAYNNNNRTG 3 cut(s) 315, 1719, 2561
Msp20I TGGCCA 1 cut(s) 2408
MspA1I CMGCKG 1 cut(s) 824
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 5 cut(s) 893, 930, 1988, 2194, 3104
MspR9I CCNGG 7 cut(s) 170, 255, 1988, 1989, 2194, 2813, 2921
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 1 cut(s) 306
MvaI CCWGG 4 cut(s) 170, 255, 2813, 2921
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 6 cut(s) 128, 259, 293, 685, 1054, 1868
NciI CCSGG 3 cut(s) 1988, 1989, 2194
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 1404
NdeII GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
NheI GCTAGC 1 cut(s) 2954
NlaIV GGNNCC 3 cut(s) 110, 1977, 2627
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 7 cut(s) 668, 733, 967, 1141, 1267, 2263, 2837
NsbI TGCGCA 1 cut(s) 1643
NspI RCATGY 4 cut(s) 1341, 2146, 2508, 3019
PaeI GCATGC 1 cut(s) 3019
PaqCI CACCTGC 1 cut(s) 1039
PceI AGGCCT 1 cut(s) 356
PctI GAATGC 1 cut(s) 306
PdmI GAANNNNTTC 3 cut(s) 20, 177, 3087
PfeI GAWTC 6 cut(s) 40, 114, 178, 1657, 2028, 2179
Pfl23II CGTACG 1 cut(s) 609
PflMI CCANNNNNTGG 3 cut(s) 827, 1422, 2609
PfoI TCCNGGA 1 cut(s) 168
PkrI GCNGC 2 cut(s) 208, 2023
PleI GAGTC 6 cut(s) 874, 1284, 1597, 1784, 1904, 2311
PmaCI CACGTG 1 cut(s) 338
PmlI CACGTG 1 cut(s) 338
PpsI GAGTC 6 cut(s) 874, 1284, 1597, 1784, 1904, 2311
Ppu21I YACGTR 3 cut(s) 338, 609, 2039
PpuMI RGGWCCY 1 cut(s) 2126
PshBI ATTAAT 2 cut(s) 536, 3041
Psp1406I AACGTT 1 cut(s) 2472
Psp5II RGGWCCY 1 cut(s) 2126
Psp6I CCWGG 4 cut(s) 168, 253, 2811, 2919
PspCI CACGTG 1 cut(s) 338
PspGI CCWGG 4 cut(s) 168, 253, 2811, 2919
PspLI CGTACG 1 cut(s) 609
PspN4I GGNNCC 3 cut(s) 110, 1977, 2627
PspPI GGNCC 5 cut(s) 597, 2126, 2191, 2625, 3098
PspPPI RGGWCCY 1 cut(s) 2126
PsrI GAACNNNNNNTAC 2 cut(s) 1378, 1410
PstI CTGCAG 3 cut(s) 216, 976, 1050
PstNI CAGNNNCTG 1 cut(s) 2267
PsuI RGATCY 3 cut(s) 1975, 2516, 3005
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 9 cut(s) 11, 611, 848, 1764, 1784, 2037, 2644, 2964, 2988
RsaNI GTAC 9 cut(s) 10, 610, 847, 1763, 1783, 2036, 2643, 2963, 2987
RseI CAYNNNNRTG 3 cut(s) 315, 1719, 2561
SatI GCNGC 2 cut(s) 207, 2022
Sau3AI GATC 9 cut(s) 124, 137, 232, 540, 950, 1164, 1975, 2516, 3005
Sau96I GGNCC 5 cut(s) 597, 2126, 2191, 2625, 3098
ScaI AGTACT 2 cut(s) 11, 1764
SchI GAGTC 6 cut(s) 875, 1285, 1598, 1784, 1904, 2312
ScrFI CCNGG 7 cut(s) 170, 255, 1988, 1989, 2194, 2813, 2921
SduI GDGCHC 3 cut(s) 343, 1318, 3036
SfcI CTRYAG 7 cut(s) 212, 521, 936, 972, 1046, 1440, 2394
SinI GGWCC 2 cut(s) 2126, 3098
SmaI CCCGGG 1 cut(s) 1989
SmiMI CAYNNNNRTG 3 cut(s) 315, 1719, 2561
SmlI CTYRAG 2 cut(s) 1661, 2150
SmoI CTYRAG 2 cut(s) 1661, 2150
SnaBI TACGTA 1 cut(s) 2039
SpeI ACTAGT 1 cut(s) 1759
SphI GCATGC 1 cut(s) 3019
SseBI AGGCCT 1 cut(s) 356
SsiI CCGC 2 cut(s) 660, 1220
SspMI CTAG 7 cut(s) 738, 965, 1601, 1760, 2744, 2891, 2955
StuI AGGCCT 1 cut(s) 356
StyD4I CCNGG 7 cut(s) 168, 253, 1986, 1987, 2192, 2811, 2919
StyI CCWWGG 4 cut(s) 367, 2658, 2710, 2890
TaiI ACGT 5 cut(s) 340, 611, 2041, 2422, 2475
TaqI TCGA 5 cut(s) 24, 181, 490, 1920, 3051
TatI WGTACW 3 cut(s) 9, 1762, 1782
TfiI GAWTC 6 cut(s) 40, 114, 178, 1657, 2028, 2179
TseFI GTSAC 7 cut(s) 668, 733, 967, 1141, 1267, 2263, 2837
TseI GCWGC 2 cut(s) 206, 2021
Tsp45I GTSAC 7 cut(s) 668, 733, 967, 1141, 1267, 2263, 2837
TspGWI ACGGA 4 cut(s) 33, 1601, 2852, 3090
TspMI CCCGGG 1 cut(s) 1987
Van91I CCANNNNNTGG 3 cut(s) 827, 1422, 2609
Vha464I CTTAAG 1 cut(s) 1661
VpaK11BI GGWCC 2 cut(s) 2126, 3098
VspI ATTAAT 2 cut(s) 536, 3041
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 4 cut(s) 266, 375, 624, 2243
XceI RCATGY 4 cut(s) 1341, 2146, 2508, 3019
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XmaI CCCGGG 1 cut(s) 1987
XmaJI CCTAGG 1 cut(s) 2890
XmiI GTMKAC 1 cut(s) 2427
XmnI GAANNNNTTC 3 cut(s) 20, 177, 3087
XspI CTAG 7 cut(s) 738, 965, 1601, 1760, 2744, 2891, 2955
ZrmI AGTACT 2 cut(s) 11, 1764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.