MD12G1080700.v1.1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002172
Physical Location & Seq
Forward (+)
9873857 .. 9881088
7232 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1080700.v1.1.491

Sequence Viewer

Length: 3120 bp
ATGGAGAAGTTCTTGAAGGACTTCGAGGTGGAGAACAAGAATCCATCGGAGGAGGCTATAAGGAGATGGAGAAGCGCCGTCGCGCTCGTCAAAAACCCTCGCAGAAGGTTTCGATTCGTCGCCGATCTCGCCAAGCGCTCCGAGGCCGAAAAGAAGAAGCTTCAAATCCAGGAAAAGATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTGCATTTCATTGATGCTGGTGATCGTGGATCTATTGAAAAGCTTGGTCAGGATGAACTCAAGCTGTCAGAAGATGCCAGAATAGCAGGTTTCAGCATTCACCCAGATGAACTTGCATCTATTACACGTGCCCATGATATTAAGGCCTTGGAAAGCCATGGTGGAATTCATGGGATTTTAAGGAAAGTCAATGTCTCGGTAGATGAAGGTGTCAAGGATAGTACCATACCAATTAGACAAAATGTTTATGGCTTAAACCGTTACAAGGAGAAAGCTCCCCGAACTTTTTGGGTGTTTGTATGGGAAGCACTACAGGACTTAACATTAATAATCCTTATGGTCTGTGCTGTGGTTTCACTTGGAGTTGGAGTTGCCACTGAAGGCTGGCCCAAAGGCACGTACGATGGTTTGGGAATTTTAATTAGTATAATTCTAGTGGTTATGGTTACCGCCATTAGTGACTACAAGCAGTCTTTGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTGTTCAGGTCACTAGGGATGGAAAAAGACAAAAAGTTTCCATTTACGACTTGGTTGTTGGAGATATTGTGCATTTGTCGATTGGGGACCAAGTTCCAGCTGATGGACTTTTCATATCTGGGTACAGTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCGGTGAATGTATCTGAAGAGAAGCCTTTTCTTCTTTCCGGAACTAAAGTGCAGGATGGGTCAGGTAAAATGCTAGCGACTACAGTTGGTATGAGGACTGAATGGGGAAAGTTGATGGAAACTCTGAGTGAAGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTTAATGGAGTCGCTACAATTATTGGTAAAATTGGTTTGACTTTTGCAGTGTTGACATTTTTGGTATTGACAGTAAGATTTTTGGTGACAAAAGGACTTAACAACGAGATCACTGATTGGTCTTCAACTGACGCCGTAACCCTTTTGAACTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACCATTAGCAGTGACGCTGAGCCTTGCTTTTGCAATGAAAAAATTGATGAATGATAGGGCACTTGTAAGGCATCTCTCAGCATGTGAGACAATGGGTTCTGCTAGTTGTATTTGCACAGATAAAACTGGAACATTAACTACAAATCATATGGTAGTTACCAAAGTATGGATATGTGAAAAAATTGTAGATGTAAAAGAAAATGACAGTAAAGAAACATTGATATCAGAAATATCTGGAGCATCAAGCATCCTTTTGCAGGTTATATTCCAAAATACGAGTTCTGAGGTTATTAAGGATGATGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCACTATTAGAGTTCGGTTTACTTTTAGGTGGCGATTTTGATACCCTGCGCAGAGAGGTTAAGATTCTTAAGATCGAACCTTTCAATTCTGTCAGGAAGAAAATGTCTGTGCTTGTAGCTTATCCTCATGGTGGAACACGAGCTTTTTGCAAAGGTGCATCAGAATTAGTACTGGGAATATGTAACAAGTACATTGACTCTAATGGGGAATCTGTTCATCTCTCCAAAGAAATGGTAGACTATATCACGGATGTCATAAATTCTCTTGCCTGTGAAGCATTGAGAACTCTCTGCTTAGCTTTTAAGGATATAGATGACTCTTCTATCGAAAATGTTATCCCAGATGATGGCTATACATTGGTAGCAGTTGTCGGTATTAAGGATCCTGTGCGCCCGGGGGTCAGGGAGGCAGTTCAGACTTGTTTAGCTGCTGGAATCACTGTACGTATGGTCACCGGTGACAATATAAATACAGCCAAAGCCATTGCTAAAGAATGTGGCATACTCACAGAGGGTGGTATAGCAATTGAAGGACCTGAGTTTCGTAGCATGTCTCTAGAGCGGATGAAAGCTGTGATACCGAAGATTCAGGTAATGGCCCGGTCTTTACCGTTGGATAAGCACACATTGGTAAAAACTTTGAGGGATGAATTTGGTGAGGTTGTTGCAGTGACTGGTGATGGGACTAACGACGCTCCCGCTTTGCATGAGTCAGACATTGGACTTGCTATGGGCATAGCTGGAACAGAGGTTGCCAAGGAAAATGCTGATGTCATCATATTGGATGACAATTTTAAAACTATAGTAAACGTGGCCAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTGCAGTTCCAGTTAACAGTTAACGTTGTCGCTCTAATGATCAATTTTGTTTCTGCATGTGTCTCAGGATCTGCTCCCCTTACAGCGGTGCAACTGCTTTGGGTGAACATGATTATGGACACTCTTGGTGCTTTGGCATTGGCAACAGAGCCTCCAAATGATGGACTTATGAAAAGGCCCCCAGTTGGGAGGGGTACCAGCTTCATCACCAAGGCTATGTGGAGAAATATCATTGGTCAGAGTATCTATCAACTGGCTGTCCTTGGAGTTCTCGATTTCTCTGGGGAGAAGCTACTAGGACTTACTGGTTCAGATGCAACTGAGGTTCTCAACACTGTGATATTCAACGCATTTGTGTTTTGCCAGGTGTTCAATCAGATAAACAGTCGTGACATAGAGAAGATAAACATATTCCGTGGAATGTTCGACAGTTGGGTATTTCTAATCGTCATGGTTTGCACAGCGGCCTTCCAGGTTATCATAGTAGAGTTCTTGGGAGCTTTTGCTAGCACTGTGCCACTAAGCTGGCAACTATGGTTACTCAGCATTTTACTTGGAGCAGTTAGCATGCTTGTTGCAGTTGTTCTCAAATTAATACCTGTTGAAAGGACAACTAAGCACCATGACGGATATGAAGCGCTTCCTTCCGGTCCTCCAGAGGGCATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1040

Amino Acids

113.47

Weight (kDa)

5.69

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 6 - 48 1.1e-19 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 122 - 189 4.1e-14 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 242 - 338 1.5e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 513 - 663 9.3e-31 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 645 - 768 8.2e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1015 5.8e-43 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1039
Acc16I TGCGCA 1 cut(s) 1643
Acc36I ACCTGC 3 cut(s) 287, 1039, 1504
Acc65I GGTACC 1 cut(s) 2642
AccB1I GGYRCC 1 cut(s) 2642
AccB7I CCANNNNNTGG 4 cut(s) 827, 1422, 1940, 2609
AccBSI CCGCTC 1 cut(s) 2155
AccI GTMKAC 1 cut(s) 1830
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 929
AciI CCGC 6 cut(s) 660, 1220, 2155, 2292, 2534, 2912
AclI AACGTT 1 cut(s) 2472
AclWI GGATC 4 cut(s) 247, 1970, 1983, 2524
AcoI YGGCCR 1 cut(s) 2406
AcsI RAATTY 4 cut(s) 375, 624, 1852, 2243
AcuI CTGAAG 2 cut(s) 609, 927
AcvI CACGTG 1 cut(s) 338
AcyI GRCGYC 1 cut(s) 1188
AfaI GTAC 8 cut(s) 433, 611, 848, 1764, 1784, 2037, 2429, 2644
AfeI AGCGCT 2 cut(s) 137, 3087
AflII CTTAAG 1 cut(s) 1661
AflIII ACRYGT 1 cut(s) 335
AgeI ACCGGT 1 cut(s) 2048
AjnI CCWGG 3 cut(s) 168, 2811, 2919
Alw26I GTCTC 5 cut(s) 409, 1031, 1337, 2151, 2515
AlwI GGATC 4 cut(s) 247, 1970, 1983, 2524
AlwNI CAGNNNCTG 2 cut(s) 2267, 2519
Ama87I CYCGRG 1 cut(s) 1987
Aor13HI TCCGGA 1 cut(s) 929
Aor51HI AGCGCT 2 cut(s) 137, 3087
AoxI GGCC 7 cut(s) 144, 354, 596, 2190, 2406, 2624, 2913
ApeKI GCWGC 2 cut(s) 206, 2021
ApoI RAATTY 4 cut(s) 375, 624, 1852, 2243
ArsI GACNNNNNNTTYG 4 cut(s) 1555, 1587, 2118, 2150
AseI ATTAAT 2 cut(s) 536, 3041
AsiGI ACCGGT 1 cut(s) 2048
Asp700I GAANNNNTTC 4 cut(s) 20, 177, 1806, 3087
Asp718I GGTACC 1 cut(s) 2642
AspLEI GCGC 6 cut(s) 77, 85, 138, 1644, 1986, 3088
AspS9I GGNCC 6 cut(s) 597, 811, 2126, 2191, 2625, 3098
AsuC2I CCSGG 3 cut(s) 1988, 1989, 2194
AsuNHI GCTAGC 2 cut(s) 964, 2954
AvaI CYCGRG 1 cut(s) 1987
AvaII GGWCC 3 cut(s) 811, 2126, 3098
BaeGI GKGCMC 2 cut(s) 343, 1318
BalI TGGCCA 1 cut(s) 2408
BamHI GGATCC 1 cut(s) 1975
BanI GGYRCC 1 cut(s) 2642
BauI CACGAG 1 cut(s) 1731
BbrPI CACGTG 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 1170
BbvI GCAGC 2 cut(s) 218, 2008
BceAI ACGGC 2 cut(s) 62, 1175
BcgI CGANNNNNNTGC 2 cut(s) 1722, 1756
BciT130I CCWGG 3 cut(s) 170, 2813, 2921
BclI TGATCA 1 cut(s) 2487
BcnI CCSGG 3 cut(s) 1988, 1989, 2194
BcoDI GTCTC 5 cut(s) 409, 1031, 1337, 2151, 2515
BfaI CTAG 7 cut(s) 644, 738, 965, 1359, 2150, 2744, 2955
BfmI CTRYAG 4 cut(s) 521, 972, 1046, 2394
BfoI RGCGCY 3 cut(s) 78, 139, 3089
BfrI CTTAAG 1 cut(s) 1661
BfuAI ACCTGC 3 cut(s) 287, 1039, 1504
BisI GCNGC 3 cut(s) 207, 2022, 2913
BlpI GCTNAGC 2 cut(s) 1274, 1888
BlsI GCNGC 3 cut(s) 208, 2023, 2914
BmcAI AGTACT 1 cut(s) 1764
Bme1390I CCNGG 6 cut(s) 170, 1988, 1989, 2194, 2813, 2921
Bme18I GGWCC 3 cut(s) 811, 2126, 3098
BmeT110I CYCGRG 1 cut(s) 1987
BmgT120I GGNCC 6 cut(s) 597, 811, 2126, 2191, 2625, 3098
BmiI GGNNCC 4 cut(s) 812, 1977, 2627, 2644
BmrFI CCNGG 6 cut(s) 170, 1988, 1989, 2194, 2813, 2921
BmrI ACTGGG 2 cut(s) 1775, 2624
BmsI GCATC 8 cut(s) 214, 274, 335, 1336, 1505, 1512, 1760, 2752
BmtI GCTAGC 2 cut(s) 968, 2958
BmuI ACTGGG 2 cut(s) 1775, 2624
BpiI GAAGAC 1 cut(s) 1170
BpmI CTGGAG 2 cut(s) 1512, 3087
Bpu1102I GCTNAGC 2 cut(s) 1274, 1888
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 3 cut(s) 1988, 1989, 2194
BsaAI YACGTR 3 cut(s) 338, 609, 2039
BsaHI GRCGYC 1 cut(s) 1188
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 9 cut(s) 141, 357, 367, 1987, 1988, 2349, 2658, 2710, 2863
BsaWI WCCGGW 3 cut(s) 929, 2048, 3095
BsaXI ACNNNNNCTCC 6 cut(s) 564, 594, 777, 807, 2584, 2614
Bse118I RCCGGY 2 cut(s) 892, 2048
Bse1I ACTGG 7 cut(s) 1387, 1770, 2272, 2458, 2630, 2706, 2758
Bse3DI GCAATG 2 cut(s) 1296, 2076
BseAI TCCGGA 1 cut(s) 929
BseBI CCWGG 3 cut(s) 170, 2813, 2921
BseDI CCNNGG 9 cut(s) 141, 357, 367, 1987, 1988, 2349, 2658, 2710, 2863
BseGI GGATG 9 cut(s) 268, 748, 952, 1503, 1558, 1849, 2163, 2245, 2383
BseMI GCAATG 2 cut(s) 1296, 2076
BseMII CTCAG 8 cut(s) 1007, 1265, 1347, 1530, 2121, 2526, 2760, 3004
BseNI ACTGG 7 cut(s) 1387, 1770, 2272, 2458, 2630, 2706, 2758
BseRI GAGGAG 2 cut(s) 65, 1041
BseSI GKGCMC 2 cut(s) 343, 1318
BseXI GCAGC 2 cut(s) 218, 2008
BsgI GTGCAG 2 cut(s) 962, 2471
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 7 cut(s) 146, 356, 598, 2192, 2408, 2626, 2915
BshNI GGYRCC 1 cut(s) 2642
BshTI ACCGGT 1 cut(s) 2048
BsiHKCI CYCGRG 1 cut(s) 1987
BsiSI CCGG 6 cut(s) 893, 930, 1988, 2049, 2194, 3096
BsiWI CGTACG 1 cut(s) 609
BslFI GGGAC 3 cut(s) 824, 2290, 2430
BsmAI GTCTC 5 cut(s) 409, 1031, 1337, 2151, 2515
BsmFI GGGAC 3 cut(s) 824, 2290, 2430
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 7 cut(s) 146, 356, 598, 2192, 2408, 2626, 2915
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 1 cut(s) 1987
Bsp1286I GDGCHC 2 cut(s) 343, 1318
Bsp13I TCCGGA 1 cut(s) 929
Bsp1407I TGTACA 1 cut(s) 2427
Bsp143I GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
Bsp1720I GCTNAGC 2 cut(s) 1274, 1888
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 6 cut(s) 660, 1220, 2155, 2292, 2534, 2912
BspANI GGCC 7 cut(s) 146, 356, 598, 2192, 2408, 2626, 2915
BspCNI CTCAG 8 cut(s) 1008, 1266, 1346, 1531, 2122, 2525, 2761, 3003
BspEI TCCGGA 1 cut(s) 929
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 4 cut(s) 812, 1977, 2627, 2644
BspMAI CTGCAG 1 cut(s) 1050
BspMI ACCTGC 3 cut(s) 287, 1039, 1504
BspOI GCTAGC 2 cut(s) 968, 2958
BspPI GGATC 4 cut(s) 247, 1970, 1983, 2524
BspT107I GGYRCC 1 cut(s) 2642
BspTI CTTAAG 1 cut(s) 1661
BspTNI GGTCTC 1 cut(s) 1031
BsrBI CCGCTC 1 cut(s) 2155
BsrDI GCAATG 2 cut(s) 1296, 2076
BsrFI RCCGGY 2 cut(s) 892, 2048
BsrGI TGTACA 1 cut(s) 2427
BsrI ACTGG 7 cut(s) 1387, 1770, 2272, 2458, 2630, 2706, 2758
BssAI RCCGGY 2 cut(s) 892, 2048
BssECI CCNNGG 9 cut(s) 141, 357, 367, 1987, 1988, 2349, 2658, 2710, 2863
BssMI GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
BssNI GRCGYC 1 cut(s) 1188
BssSI CACGAG 1 cut(s) 1731
BssT1I CCWWGG 5 cut(s) 357, 367, 2349, 2658, 2710
Bst2BI CACGAG 1 cut(s) 1731
Bst2UI CCWGG 3 cut(s) 170, 2813, 2921
Bst6I CTCTTC 2 cut(s) 903, 1918
BstACI GRCGYC 1 cut(s) 1188
BstAFI CTTAAG 1 cut(s) 1661
BstAPI GCANNNNNTGC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 2427
BstBAI YACGTR 3 cut(s) 338, 609, 2039
BstC8I GCNNGC 5 cut(s) 596, 966, 2956, 2975, 3017
BstDSI CCRYGG 2 cut(s) 367, 2863
BstEII GGTNACC 2 cut(s) 655, 2044
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 9 cut(s) 268, 748, 952, 1503, 1558, 1849, 2163, 2245, 2383
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 3 cut(s) 78, 139, 3089
BstHHI GCGC 6 cut(s) 77, 85, 138, 1644, 1986, 3088
BstKTI GATC 8 cut(s) 127, 235, 242, 1167, 1668, 1978, 2490, 2519
BstMAI GTCTC 5 cut(s) 409, 1031, 1337, 2151, 2515
BstMBI GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
BstMWI GCNNNNNNNGC 5 cut(s) 128, 293, 685, 1054, 1868
BstNI CCWGG 3 cut(s) 170, 2813, 2921
BstNSI RCATGY 4 cut(s) 1341, 2146, 2508, 3019
BstPI GGTNACC 2 cut(s) 655, 2044
BstSCI CCNGG 6 cut(s) 168, 1986, 1987, 2192, 2811, 2919
BstSFI CTRYAG 4 cut(s) 521, 972, 1046, 2394
BstSLI GKGCMC 2 cut(s) 343, 1318
BstSNI TACGTA 1 cut(s) 2039
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 218, 2008
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 3 cut(s) 239, 1975, 2516
BstXI CCANNNNNNTGG 2 cut(s) 1825, 2973
BstYI RGATCY 3 cut(s) 239, 1975, 2516
BsuRI GGCC 7 cut(s) 146, 356, 598, 2192, 2408, 2626, 2915
BtgI CCRYGG 2 cut(s) 367, 2863
BtsCI GGATG 9 cut(s) 268, 748, 952, 1503, 1558, 1849, 2163, 2245, 2383
BtsI GCAGTG 5 cut(s) 209, 1043, 1110, 1272, 2268
BveI ACCTGC 3 cut(s) 287, 1039, 1504
Cac8I GCNNGC 5 cut(s) 596, 966, 2956, 2975, 3017
CaiI CAGNNNCTG 2 cut(s) 2267, 2519
CfoI GCGC 6 cut(s) 77, 85, 138, 1644, 1986, 3088
Cfr10I RCCGGY 2 cut(s) 892, 2048
Cfr13I GGNCC 6 cut(s) 597, 811, 2126, 2191, 2625, 3098
Cfr9I CCCGGG 1 cut(s) 1987
CseI GACGC 3 cut(s) 1196, 1279, 2294
Csp6I GTAC 8 cut(s) 432, 610, 847, 1763, 1783, 2036, 2428, 2643
CspAI ACCGGT 1 cut(s) 2048
CviQI GTAC 8 cut(s) 432, 610, 847, 1763, 1783, 2036, 2428, 2643
DpnI GATC 8 cut(s) 126, 234, 241, 1166, 1667, 1977, 2489, 2518
DpnII GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
DraI TTTAAA 1 cut(s) 2389
EaeI YGGCCR 1 cut(s) 2406
Eam1104I CTCTTC 2 cut(s) 903, 1918
EarI CTCTTC 2 cut(s) 903, 1918
Eco105I TACGTA 1 cut(s) 2039
Eco130I CCWWGG 5 cut(s) 357, 367, 2349, 2658, 2710
Eco147I AGGCCT 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 1031
Eco32I GATATC 1 cut(s) 1479
Eco47I GGWCC 3 cut(s) 811, 2126, 3098
Eco47III AGCGCT 2 cut(s) 137, 3087
Eco57I CTGAAG 2 cut(s) 609, 927
Eco72I CACGTG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 1987
Eco91I GGTNACC 2 cut(s) 655, 2044
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 2 cut(s) 2126, 2625
EcoO65I GGTNACC 2 cut(s) 655, 2044
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 3 cut(s) 168, 2811, 2919
EcoRV GATATC 1 cut(s) 1479
EcoT14I CCWWGG 5 cut(s) 357, 367, 2349, 2658, 2710
ErhI CCWWGG 5 cut(s) 357, 367, 2349, 2658, 2710
FaqI GGGAC 3 cut(s) 824, 2290, 2430
FauI CCCGC 1 cut(s) 2299
FauNDI CATATG 1 cut(s) 1404
FbaI TGATCA 1 cut(s) 2487
FblI GTMKAC 1 cut(s) 1830
Fnu4HI GCNGC 3 cut(s) 207, 2022, 2913
FokI GGATG 9 cut(s) 275, 755, 959, 1490, 1565, 1856, 2170, 2252, 2390
Fsp4HI GCNGC 3 cut(s) 207, 2022, 2913
FspBI CTAG 7 cut(s) 644, 738, 965, 1359, 2150, 2744, 2955
FspI TGCGCA 1 cut(s) 1643
GlaI GCGC 6 cut(s) 76, 84, 137, 1643, 1985, 3087
GluI GCNGC 3 cut(s) 207, 2022, 2913
GsuI CTGGAG 2 cut(s) 1512, 3087
HaeII RGCGCY 3 cut(s) 78, 139, 3089
HaeIII GGCC 7 cut(s) 146, 356, 598, 2192, 2408, 2626, 2915
HapII CCGG 6 cut(s) 893, 930, 1988, 2049, 2194, 3096
HgaI GACGC 3 cut(s) 1196, 1279, 2294
HhaI GCGC 6 cut(s) 77, 85, 138, 1644, 1986, 3088
Hin1I GRCGYC 1 cut(s) 1188
Hin6I GCGC 6 cut(s) 75, 83, 136, 1642, 1984, 3086
HinP1I GCGC 6 cut(s) 75, 83, 136, 1642, 1984, 3086
HincII GTYRAC 3 cut(s) 1110, 2463, 2470
HindII GTYRAC 3 cut(s) 1110, 2463, 2470
HindIII AAGCTT 4 cut(s) 158, 251, 871, 1055
HpaI GTTAAC 2 cut(s) 2463, 2470
HpaII CCGG 6 cut(s) 893, 930, 1988, 2049, 2194, 3096
Hpy166II GTNNAC 8 cut(s) 1110, 1613, 1831, 2401, 2428, 2463, 2470, 2554
Hpy8I GTNNAC 8 cut(s) 1110, 1613, 1831, 2401, 2428, 2463, 2470, 2554
Hpy99I CGWCG 3 cut(s) 83, 122, 2288
HpyAV CCTTC 9 cut(s) 10, 99, 410, 584, 1016, 1244, 2117, 2926, 3102
HpyCH4IV ACGT 6 cut(s) 337, 608, 2038, 2403, 2419, 2472
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 293, 685, 1054, 1868
HpySE526I ACGT 6 cut(s) 337, 608, 2038, 2403, 2419, 2472
Hsp92I GRCGYC 1 cut(s) 1188
HspAI GCGC 6 cut(s) 75, 83, 136, 1642, 1984, 3086
Kpn2I TCCGGA 1 cut(s) 929
KpnI GGTACC 1 cut(s) 2646
Ksp22I TGATCA 1 cut(s) 2487
KspAI GTTAAC 2 cut(s) 2463, 2470
Kzo9I GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
LmnI GCTCC 7 cut(s) 143, 490, 1493, 2293, 2527, 2945, 3005
Lsp1109I GCAGC 2 cut(s) 218, 2008
LweI GCATC 8 cut(s) 214, 274, 335, 1336, 1505, 1512, 1760, 2752
MaeI CTAG 7 cut(s) 644, 738, 965, 1359, 2150, 2744, 2955
MaeII ACGT 6 cut(s) 337, 608, 2038, 2403, 2419, 2472
MalI GATC 8 cut(s) 126, 234, 241, 1166, 1667, 1977, 2489, 2518
MbiI CCGCTC 1 cut(s) 2155
MboI GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
MfeI CAATTG 1 cut(s) 2118
MflI RGATCY 3 cut(s) 239, 1975, 2516
MhlI GDGCHC 2 cut(s) 343, 1318
MlsI TGGCCA 1 cut(s) 2408
MluNI TGGCCA 1 cut(s) 2408
MlyI GAGTC 6 cut(s) 875, 1074, 1598, 1784, 1904, 2312
MmeI TCCRAC 3 cut(s) 556, 763, 2187
Mox20I TGGCCA 1 cut(s) 2408
MroI TCCGGA 1 cut(s) 929
MroXI GAANNNNTTC 4 cut(s) 20, 177, 1806, 3087
MscI TGGCCA 1 cut(s) 2408
MslI CAYNNNNRTG 2 cut(s) 315, 2561
Msp20I TGGCCA 1 cut(s) 2408
MspA1I CMGCKG 3 cut(s) 824, 2534, 2912
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 6 cut(s) 893, 930, 1988, 2049, 2194, 3096
MspR9I CCNGG 6 cut(s) 170, 1988, 1989, 2194, 2813, 2921
MunI CAATTG 1 cut(s) 2118
Mva1269I GAATGC 1 cut(s) 306
MvaI CCWGG 3 cut(s) 170, 2813, 2921
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 5 cut(s) 128, 293, 685, 1054, 1868
NciI CCSGG 3 cut(s) 1988, 1989, 2194
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 1404
NdeII GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
NheI GCTAGC 2 cut(s) 964, 2954
NlaIV GGNNCC 4 cut(s) 812, 1977, 2627, 2644
NmuCI GTSAC 8 cut(s) 668, 733, 1141, 1267, 2044, 2051, 2263, 2837
NsbI TGCGCA 1 cut(s) 1643
NspI RCATGY 4 cut(s) 1341, 2146, 2508, 3019
PaeI GCATGC 1 cut(s) 3019
PaqCI CACCTGC 1 cut(s) 1039
PceI AGGCCT 1 cut(s) 356
PctI GAATGC 1 cut(s) 306
PdmI GAANNNNTTC 4 cut(s) 20, 177, 1806, 3087
PfeI GAWTC 7 cut(s) 40, 114, 178, 1657, 1802, 2028, 2179
Pfl23II CGTACG 1 cut(s) 609
PflMI CCANNNNNTGG 4 cut(s) 827, 1422, 1940, 2609
PfoI TCCNGGA 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 2048
PkrI GCNGC 3 cut(s) 208, 2023, 2914
PleI GAGTC 6 cut(s) 874, 1073, 1597, 1784, 1904, 2311
PmaCI CACGTG 1 cut(s) 338
PmlI CACGTG 1 cut(s) 338
PpsI GAGTC 6 cut(s) 874, 1073, 1597, 1784, 1904, 2311
Ppu21I YACGTR 3 cut(s) 338, 609, 2039
PpuMI RGGWCCY 1 cut(s) 2126
PshBI ATTAAT 2 cut(s) 536, 3041
Psp1406I AACGTT 1 cut(s) 2472
Psp5II RGGWCCY 1 cut(s) 2126
Psp6I CCWGG 3 cut(s) 168, 2811, 2919
PspCI CACGTG 1 cut(s) 338
PspEI GGTNACC 2 cut(s) 655, 2044
PspGI CCWGG 3 cut(s) 168, 2811, 2919
PspLI CGTACG 1 cut(s) 609
PspN4I GGNNCC 4 cut(s) 812, 1977, 2627, 2644
PspPI GGNCC 6 cut(s) 597, 811, 2126, 2191, 2625, 3098
PspPPI RGGWCCY 1 cut(s) 2126
PsrI GAACNNNNNNTAC 2 cut(s) 1378, 1410
PstI CTGCAG 1 cut(s) 1050
PstNI CAGNNNCTG 2 cut(s) 2267, 2519
PsuI RGATCY 3 cut(s) 239, 1975, 2516
PvuII CAGCTG 1 cut(s) 824
RsaI GTAC 8 cut(s) 433, 611, 848, 1764, 1784, 2037, 2429, 2644
RsaNI GTAC 8 cut(s) 432, 610, 847, 1763, 1783, 2036, 2428, 2643
RseI CAYNNNNRTG 2 cut(s) 315, 2561
SatI GCNGC 3 cut(s) 207, 2022, 2913
Sau3AI GATC 8 cut(s) 124, 232, 239, 1164, 1665, 1975, 2487, 2516
Sau96I GGNCC 6 cut(s) 597, 811, 2126, 2191, 2625, 3098
ScaI AGTACT 1 cut(s) 1764
SchI GAGTC 6 cut(s) 875, 1074, 1598, 1784, 1904, 2312
ScrFI CCNGG 6 cut(s) 170, 1988, 1989, 2194, 2813, 2921
SduI GDGCHC 2 cut(s) 343, 1318
SfaNI GCATC 8 cut(s) 214, 274, 335, 1336, 1505, 1512, 1760, 2752
SfcI CTRYAG 4 cut(s) 521, 972, 1046, 2394
SgrAI CRCCGGYG 1 cut(s) 2048
SinI GGWCC 3 cut(s) 811, 2126, 3098
SmaI CCCGGG 1 cut(s) 1989
SmiMI CAYNNNNRTG 2 cut(s) 315, 2561
SmlI CTYRAG 2 cut(s) 269, 1661
SmoI CTYRAG 2 cut(s) 269, 1661
SnaBI TACGTA 1 cut(s) 2039
SphI GCATGC 1 cut(s) 3019
SseBI AGGCCT 1 cut(s) 356
SsiI CCGC 6 cut(s) 660, 1220, 2155, 2292, 2534, 2912
SspMI CTAG 7 cut(s) 644, 738, 965, 1359, 2150, 2744, 2955
StuI AGGCCT 1 cut(s) 356
StyD4I CCNGG 6 cut(s) 168, 1986, 1987, 2192, 2811, 2919
StyI CCWWGG 5 cut(s) 357, 367, 2349, 2658, 2710
TaiI ACGT 6 cut(s) 340, 611, 2041, 2406, 2422, 2475
TaqI TCGA 8 cut(s) 24, 112, 181, 803, 1668, 1920, 2721, 2874
TatI WGTACW 3 cut(s) 1762, 1782, 2427
TauI GCSGC 1 cut(s) 2915
TfiI GAWTC 7 cut(s) 40, 114, 178, 1657, 1802, 2028, 2179
TseFI GTSAC 8 cut(s) 668, 733, 1141, 1267, 2044, 2051, 2263, 2837
TseI GCWGC 2 cut(s) 206, 2021
Tsp45I GTSAC 8 cut(s) 668, 733, 1141, 1267, 2044, 2051, 2263, 2837
TspGWI ACGGA 3 cut(s) 1856, 2852, 3090
TspMI CCCGGG 1 cut(s) 1987
Van91I CCANNNNNTGG 4 cut(s) 827, 1422, 1940, 2609
Vha464I CTTAAG 1 cut(s) 1661
VpaK11BI GGWCC 3 cut(s) 811, 2126, 3098
VspI ATTAAT 2 cut(s) 536, 3041
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 4 cut(s) 375, 624, 1852, 2243
XbaI TCTAGA 1 cut(s) 2149
XceI RCATGY 4 cut(s) 1341, 2146, 2508, 3019
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XmaI CCCGGG 1 cut(s) 1987
XmiI GTMKAC 1 cut(s) 1830
XmnI GAANNNNTTC 4 cut(s) 20, 177, 1806, 3087
XspI CTAG 7 cut(s) 644, 738, 965, 1359, 2150, 2744, 2955
ZrmI AGTACT 1 cut(s) 1764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.