Rroxscaffold_6G00398310

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002172
Physical Location & Seq
Reverse (-)
20190024 .. 20197658
7635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398310.1

Sequence Viewer

Length: 3120 bp
ATGGAAAGCTACTTGCACAAGGACTTCGACGTCCAGCACAAGCACCTCTCGCCGGAGGCCTCAAGCAATTGGAGGAGAGCCGTCGGCCGCGTCGTCAAGAACCCCGCCAGAAGGTTCCGACATGTCGCCGATCTTGCCAAGCGCGCCGAGGGTGAGAGGAAGAAGAAGCAAATCCAGGAAAAAATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTAACATTCATTGAGGCTGGAGGTAGCCAACCAGCTACTGATCAGGTTGAATACAAACTGTCTGAAGAGGCTGAAAAAGAGGGTTTCAGCATTCACCCAGATGAACTTGCATCAATCATACGCAGCCATGATATTAAGGCCTTGGAAAGCCATGGTGGAGTTGATGGGATTTTAAAAAAGTTATCCGTCATACCAGATGAAGGTGTCAGGGGTAGCAGTATGCCAACAAGGCAAAATGTTTATGGCTTAAACCGCTATACGGAGAAACCTCCCCGATCATTCTTGGTGTTTGTATGGGAAGCTCTACAGGATTTAACTTTAATTATTCTTATGGTTTGTGCCGTGGTTTCAATTGGAGTGGGGCTTGCTACTGACGGGTGGCCAGAAGGCATGTATGATGGGGTGGGAATTTTACTCAGTATAGTTTTAGTTGTTTTGGTCACTGCCATTAGTGATTACAGGCAGTCCATGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTATTCAGGTCACTAGGGATGGGAAAAGACAAAAAGTTTCCATTTATGATTTGCTGGTGGGAGATATTGTTCATTTGGCAGTGGGTGACCTAGTTCCAGCAGATGGACTATTCATATCAGGATACAGCTTGTTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGACTGTGTATGAAGAAAAGCCTTTTCTTCTTTCGGGAACCAAAGTGCAAGATGGGTCAGGTAAAATGTTGGTAACAACAGTTGGTATGAGGACCGAATGGGGAAAGTTGATGGAAACTCTGAGTGAGGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGGCTTTCGCTGTGGTGACATTTTTGGTTTTGACAGTAAGATATTTGGTGGAAAAGGCACTTAGCAATGAGATCACAGATTGGTCTTCAACTGATGCTGTAACACTTTTGAACTACTTTGCCATTGCAGTAACTATAATTGTTGTTGCAGTTCCTGAAGGTTTACCATTGGCAGTAACACTGAGTCTTGCTTTTGCAATGAAGAAATTAATGAATGATAAGGCACTTGTGAGGCATCTCTCCGCCTGTGAGACAATGGGTTCTGCTAGTTGCATATGCACGGATAAAACAGGAACATTAACCACAAACCATATGGTAGTTACCAAGATATGGGTAGGTGACAAATCTATAAATGTAAAAGGTAATGAAAGTAAAGACGTAGTAAAATCAGAAATTTCTGGAGCACTGGACATCCTATTGCAGGTTATTTTTCAAAATACAAGCTCTGAGGTTATCAAGGATGAGGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCATTATTAGAATTTGGTTTGCTTTTGGGTGGCGATTTTGATGCCCAGCGCAGAGAGGTTAAGATTCTTAAGATGGAACCTTTCAATTCTGTTAGGAAGAAGATGTCTGTGCTCATCGCTCATCCTTATGGTGGGATAAGAGCTTTTTGCAAAGGTGCATCTGAAATAGTATTGGGAATGTGCAACAAAGTTATTGACTGTAATGGAGAATCTGTTGATCTCTCCCGAAAAGAGGCAAATAATATCACAGATGTCATAAATTCTTTTGCTTCTGAAGCATTAAGAACTCTCTGCTTGGCTTTTAAAGATATACATGACTCTTCCATCAACAATGACATCCCCAATGATGGCTATACATTGATAGCAGTTGTAGGAATCAAGGATCCTGTGCGTCCTGGGGTGAAGGAAGCAGTCCAAACTTGTTTGGCTGCTGGGATAACTGTACGAATGGTCACAGGTGACAATATAAATACTGCTAAAGCCATAGCTAGAGAATGTGGCATACTCACAGAGAACGGTTTGGCAATAGAAGGACCAGAATTTCGTAACATGTCTCCTGCAGATATGAAAGCTGTTATACCGAGGATTCAGGTGATGGCCCGATCCTTACCATTGGATAAGCACACCTTGGTGAAGAATTTGAGGAATACATTCCAGGAGGTAGTTGCAGTTACAGGTGATGGGACCAATGACGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGCACAGAGGTTGCTAAAGAAAGTGCTGATGTCATCGTATTGGATGACAATTTTAGTACTATAGTAAATGTGGCCAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTCCAGTTCCAGTTAACAGTGAACGTTGTTGCTCTGATGATCAATTTTGTTTCTGCCTGTGTATCAGGTGATGCTCCCCTTACTGCTGTGCAATTACTCTGGGTCAACATGATTATGGACACTCTTGGTGCTTTGGCACTGGCTACCGAGCCTCCAAATGAAGGACTGATGAAAAGGCCCCCTGTTTCAAGGGGTACAAGCTTCATCACCAGGCCCATGTGGAGGAATATCATTGGTCAGAGCATTTATCAATTGGCTGTTCTTGGAGTTCTCGATTTCCGTGGAACGCAGCTGCTAGGACTCACTGGTTCAGATGCAACTGATATTCTTGACACTGTGATATTCAATGCATTCGTGTTCTGCCAGGTGTTCAATCAGATAAACAGCCGTGATATAGAGAAGATAAACATATTCCGTGGAATGTTTGACAGCTGGGTATTCCTAGTGGTCATGGTTTGCACAGTGGGCTTCCAAATTGTCCTAGTAGAGTTCTTGGGCGCGTTTGCTAGCACTGTACCCCTGAGCTGGCAGCTATGGTTACTCTGCATTATACTTGGATTGATTAGCATGCCTGTTGCAGTTGTCGTGAAATGCATACCCGTTGAAAGCAAAGTTAAACCCCCTGATGGCTATGTAACTATTCCTGATGGTCCTCCAGCCGGCCTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1039

Amino Acids

113.16

Weight (kDa)

5.67

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 5 - 50 5.3e-15 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 122 - 189 9.7e-13 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 244 - 338 6.9e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 513 - 663 2.5e-31 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 650 - 770 1.2e-11 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1014 2.1e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1039
AasI GACNNNNNNGTC 1 cut(s) 29
AatII GACGTC 1 cut(s) 33
Acc36I ACCTGC 2 cut(s) 1039, 1504
AccB7I CCANNNNNTGG 2 cut(s) 827, 1422
AccII CGCG 3 cut(s) 90, 144, 2948
AciI CCGC 4 cut(s) 88, 105, 469, 1335
AclI AACGTT 1 cut(s) 2472
AclWI GGATC 3 cut(s) 1970, 1983, 2190
AcoI YGGCCR 3 cut(s) 85, 596, 2406
AcsI RAATTY 7 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2230
AcuI CTGAAG 3 cut(s) 300, 1269, 1887
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 5 cut(s) 2037, 2392, 2429, 2644, 2964
AflII CTTAAG 1 cut(s) 1661
AflIII ACRYGT 2 cut(s) 121, 2142
AjnI CCWGG 5 cut(s) 174, 1987, 2247, 2657, 2811
AleI CACNNNNGTG 1 cut(s) 2222
AloI GAACNNNNNNTCC 2 cut(s) 777, 809
Alw21I GWGCWC 2 cut(s) 1498, 1707
Alw26I GTCTC 3 cut(s) 1031, 1337, 2151
AlwI GGATC 3 cut(s) 1970, 1983, 2190
AlwNI CAGNNNCTG 3 cut(s) 254, 899, 1247
AoxI GGCC 9 cut(s) 57, 85, 354, 596, 2190, 2406, 2624, 2660, 3109
ApeKI GCWGC 6 cut(s) 212, 339, 2021, 2737, 2740, 2977
ApoI RAATTY 7 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2230
ArsI GACNNNNNNTTYG 4 cut(s) 1555, 1587, 2118, 2150
AseI ATTAAT 1 cut(s) 1301
Asp700I GAANNNNTTC 2 cut(s) 183, 2243
AspLEI GCGC 4 cut(s) 144, 146, 1644, 2948
AspS9I GGNCC 7 cut(s) 987, 2126, 2191, 2277, 2625, 2661, 3098
AsuNHI GCTAGC 1 cut(s) 2954
AvaII GGWCC 4 cut(s) 987, 2126, 2277, 3098
BalI TGGCCA 2 cut(s) 598, 2408
BamHI GGATCC 1 cut(s) 1975
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 2 cut(s) 1498, 1707
BbvI GCAGC 6 cut(s) 224, 351, 2008, 2727, 2749, 2989
BceAI ACGGC 3 cut(s) 65, 542, 2820
BcgI CGANNNNNNTGC 2 cut(s) 1616, 1650
BciT130I CCWGG 5 cut(s) 176, 1989, 2249, 2659, 2813
BciVI GTATCC 1 cut(s) 839
BclI TGATCA 2 cut(s) 256, 2487
BcoDI GTCTC 3 cut(s) 1031, 1337, 2151
BfaI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
BfmI CTRYAG 4 cut(s) 521, 1046, 2151, 2394
BfrI CTTAAG 1 cut(s) 1661
BfuAI ACCTGC 2 cut(s) 1039, 1504
BfuI GTATCC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 445
BisI GCNGC 7 cut(s) 88, 213, 340, 2022, 2738, 2741, 2978
BlsI GCNGC 7 cut(s) 89, 214, 341, 2023, 2739, 2742, 2979
BmcAI AGTACT 1 cut(s) 2392
Bme1390I CCNGG 5 cut(s) 176, 1989, 2249, 2659, 2813
Bme18I GGWCC 4 cut(s) 987, 2126, 2277, 3098
BmgT120I GGNCC 7 cut(s) 987, 2126, 2191, 2277, 2625, 2661, 3098
BmiI GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
BmrFI CCNGG 5 cut(s) 176, 1989, 2249, 2659, 2813
BmsI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
BmtI GCTAGC 1 cut(s) 2958
BpiI GAAGAC 1 cut(s) 1170
BpmI CTGGAG 3 cut(s) 255, 1512, 3087
Bpu10I CCTNAGC 1 cut(s) 2969
BpuEI CTTGAG 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 9 cut(s) 147, 357, 367, 558, 1988, 2174, 2220, 2728, 2863
BsaXI ACNNNNNCTCC 6 cut(s) 777, 807, 1791, 1821, 2584, 2614
Bse118I RCCGGY 1 cut(s) 3107
Bse1I ACTGG 6 cut(s) 893, 1503, 2452, 2458, 2592, 2758
Bse3DI GCAATG 3 cut(s) 1165, 1215, 1296
BseBI CCWGG 5 cut(s) 176, 1989, 2249, 2659, 2813
BseDI CCNNGG 9 cut(s) 147, 357, 367, 558, 1988, 2174, 2220, 2728, 2863
BseGI GGATG 6 cut(s) 748, 1503, 1558, 1714, 1929, 2383
BseMI GCAATG 3 cut(s) 1165, 1215, 1296
BseMII CTCAG 5 cut(s) 646, 1007, 1265, 1530, 2960
BseNI ACTGG 6 cut(s) 893, 1503, 2452, 2458, 2592, 2758
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 2 cut(s) 88, 1041
BseX3I CGGCCG 1 cut(s) 85
BseXI GCAGC 6 cut(s) 224, 351, 2008, 2727, 2749, 2989
BseYI CCCAGC 3 cut(s) 1638, 2024, 2880
Bsh1236I CGCG 3 cut(s) 90, 144, 2948
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 9 cut(s) 59, 87, 356, 598, 2192, 2408, 2626, 2662, 3111
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 1498, 1707
BsiSI CCGG 2 cut(s) 53, 3108
BslFI GGGAC 2 cut(s) 2290, 2430
BsmAI GTCTC 3 cut(s) 1031, 1337, 2151
BsmFI GGGAC 2 cut(s) 2290, 2430
BsmI GAATGC 2 cut(s) 306, 2798
BsnI GGCC 9 cut(s) 59, 87, 356, 598, 2192, 2408, 2626, 2662, 3111
Bso31I GGTCTC 1 cut(s) 1031
Bsp1286I GDGCHC 2 cut(s) 1498, 1707
Bsp1407I TGTACA 1 cut(s) 2427
Bsp143I GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 4 cut(s) 88, 105, 469, 1335
BspANI GGCC 9 cut(s) 59, 87, 356, 598, 2192, 2408, 2626, 2662, 3111
BspCNI CTCAG 5 cut(s) 645, 1008, 1266, 1531, 2961
BspFNI CGCG 3 cut(s) 90, 144, 2948
BspLI GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
BspMAI CTGCAG 2 cut(s) 1050, 2155
BspMI ACCTGC 2 cut(s) 1039, 1504
BspOI GCTAGC 1 cut(s) 2958
BspPI GGATC 3 cut(s) 1970, 1983, 2190
BspTI CTTAAG 1 cut(s) 1661
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 3 cut(s) 1165, 1215, 1296
BsrFI RCCGGY 1 cut(s) 3107
BsrGI TGTACA 1 cut(s) 2427
BsrI ACTGG 6 cut(s) 893, 1503, 2452, 2458, 2592, 2758
BssAI RCCGGY 1 cut(s) 3107
BssECI CCNNGG 9 cut(s) 147, 357, 367, 558, 1988, 2174, 2220, 2728, 2863
BssHII GCGCGC 1 cut(s) 142
BssMI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 3 cut(s) 357, 367, 2220
Bst2UI CCWGG 5 cut(s) 176, 1989, 2249, 2659, 2813
Bst6I CTCTTC 2 cut(s) 276, 1918
BstACI GRCGYC 1 cut(s) 30
BstAFI CTTAAG 1 cut(s) 1661
BstAPI GCANNNNNTGC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 2427
BstC8I GCNNGC 7 cut(s) 144, 582, 2335, 2956, 2975, 3017, 3109
BstDEI CTNAG 6 cut(s) 632, 1016, 1154, 1274, 1539, 2969
BstDSI CCRYGG 4 cut(s) 367, 558, 2728, 2863
BstEII GGTNACC 1 cut(s) 809
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 6 cut(s) 748, 1503, 1558, 1714, 1929, 2383
BstFNI CGCG 3 cut(s) 90, 144, 2948
BstHHI GCGC 4 cut(s) 144, 146, 1644, 2948
BstKTI GATC 8 cut(s) 133, 259, 494, 1167, 1813, 1978, 2198, 2490
BstMAI GTCTC 3 cut(s) 1031, 1337, 2151
BstMBI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
BstMCI CGRYCG 1 cut(s) 88
BstNI CCWGG 5 cut(s) 176, 1989, 2249, 2659, 2813
BstNSI RCATGY 4 cut(s) 125, 610, 2146, 3019
BstPI GGTNACC 1 cut(s) 809
BstSCI CCNGG 5 cut(s) 174, 1987, 2247, 2657, 2811
BstSFI CTRYAG 4 cut(s) 521, 1046, 2151, 2394
BstUI CGCG 3 cut(s) 90, 144, 2948
BstV1I GCAGC 6 cut(s) 224, 351, 2008, 2727, 2749, 2989
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 1 cut(s) 1975
BstYI RGATCY 1 cut(s) 1975
BstZI CGGCCG 1 cut(s) 85
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 9 cut(s) 59, 87, 356, 598, 2192, 2408, 2626, 2662, 3111
BtgI CCRYGG 4 cut(s) 367, 558, 2728, 2863
BtgZI GCGATG 1 cut(s) 1693
BtsCI GGATG 6 cut(s) 748, 1503, 1558, 1714, 1929, 2383
BtsI GCAGTG 3 cut(s) 657, 810, 1043
BveI ACCTGC 2 cut(s) 1039, 1504
Cac8I GCNNGC 7 cut(s) 144, 582, 2335, 2956, 2975, 3017, 3109
CaiI CAGNNNCTG 3 cut(s) 254, 899, 1247
CfoI GCGC 4 cut(s) 144, 146, 1644, 2948
Cfr10I RCCGGY 1 cut(s) 3107
Cfr13I GGNCC 7 cut(s) 987, 2126, 2191, 2277, 2625, 2661, 3098
CseI GACGC 3 cut(s) 79, 1973, 2294
Csp6I GTAC 5 cut(s) 2036, 2391, 2428, 2643, 2963
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviQI GTAC 5 cut(s) 2036, 2391, 2428, 2643, 2963
DdeI CTNAG 6 cut(s) 632, 1016, 1154, 1274, 1539, 2969
DpnI GATC 8 cut(s) 132, 258, 493, 1166, 1812, 1977, 2197, 2489
DpnII GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
DraI TTTAAA 2 cut(s) 390, 1897
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 3 cut(s) 85, 596, 2406
EagI CGGCCG 1 cut(s) 85
Eam1104I CTCTTC 2 cut(s) 276, 1918
EarI CTCTTC 2 cut(s) 276, 1918
EciI GGCGGA 1 cut(s) 1324
EclXI CGGCCG 1 cut(s) 85
Eco130I CCWWGG 3 cut(s) 357, 367, 2220
Eco147I AGGCCT 2 cut(s) 59, 356
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 4 cut(s) 987, 2126, 2277, 3098
Eco52I CGGCCG 1 cut(s) 85
Eco57I CTGAAG 3 cut(s) 300, 1269, 1887
Eco91I GGTNACC 1 cut(s) 809
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 1 cut(s) 2625
EcoO65I GGTNACC 1 cut(s) 809
EcoRII CCWGG 5 cut(s) 174, 1987, 2247, 2657, 2811
EcoT14I CCWWGG 3 cut(s) 357, 367, 2220
EcoT22I ATGCAT 2 cut(s) 2800, 3044
ErhI CCWWGG 3 cut(s) 357, 367, 2220
FalI AAGNNNNNCTT 2 cut(s) 2204, 2236
FaqI GGGAC 2 cut(s) 2290, 2430
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 2 cut(s) 1367, 1404
FbaI TGATCA 2 cut(s) 256, 2487
Fnu4HI GCNGC 7 cut(s) 88, 213, 340, 2022, 2738, 2741, 2978
FokI GGATG 6 cut(s) 755, 1490, 1565, 1701, 1916, 2390
Fsp4HI GCNGC 7 cut(s) 88, 213, 340, 2022, 2738, 2741, 2978
FspBI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
GlaI GCGC 4 cut(s) 143, 145, 1643, 2947
GluI GCNGC 7 cut(s) 88, 213, 340, 2022, 2738, 2741, 2978
GsaI CCCAGC 3 cut(s) 1642, 2028, 2884
GsuI CTGGAG 3 cut(s) 255, 1512, 3087
HaeIII GGCC 9 cut(s) 59, 87, 356, 598, 2192, 2408, 2626, 2662, 3111
HapII CCGG 2 cut(s) 53, 3108
HgaI GACGC 3 cut(s) 79, 1973, 2294
HhaI GCGC 4 cut(s) 144, 146, 1644, 2948
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 4 cut(s) 142, 144, 1642, 2946
HinP1I GCGC 4 cut(s) 142, 144, 1642, 2946
HincII GTYRAC 2 cut(s) 2463, 2554
HindII GTYRAC 2 cut(s) 2463, 2554
HindIII AAGCTT 2 cut(s) 871, 2647
HinfI GANTC 9 cut(s) 866, 1276, 1589, 1657, 1802, 1910, 1968, 2179, 2748
HpaI GTTAAC 1 cut(s) 2463
HpaII CCGG 2 cut(s) 53, 3108
Hpy166II GTNNAC 5 cut(s) 1256, 2428, 2463, 2470, 2554
Hpy8I GTNNAC 5 cut(s) 1256, 2428, 2463, 2470, 2554
Hpy99I CGWCG 3 cut(s) 32, 86, 95
HpyAV CCTTC 7 cut(s) 105, 410, 596, 1244, 1990, 2117, 2603
HpyCH4IV ACGT 4 cut(s) 30, 1470, 2419, 2472
HpyF3I CTNAG 6 cut(s) 632, 1016, 1154, 1274, 1539, 2969
HpySE526I ACGT 4 cut(s) 30, 1470, 2419, 2472
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 4 cut(s) 142, 144, 1642, 2946
KroI GCCGGC 1 cut(s) 3107
KroNI GCCGGC 1 cut(s) 3109
Ksp22I TGATCA 2 cut(s) 256, 2487
KspAI GTTAAC 1 cut(s) 2463
Kzo9I GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
LmnI GCTCC 3 cut(s) 1493, 2293, 2527
Lsp1109I GCAGC 6 cut(s) 224, 351, 2008, 2727, 2749, 2989
LweI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
MaeI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
MaeII ACGT 4 cut(s) 30, 1470, 2419, 2472
MalI GATC 8 cut(s) 132, 258, 493, 1166, 1812, 1977, 2197, 2489
MboI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
MfeI CAATTG 3 cut(s) 67, 567, 2699
MflI RGATCY 1 cut(s) 1975
MhlI GDGCHC 2 cut(s) 1498, 1707
MlsI TGGCCA 2 cut(s) 598, 2408
MluNI TGGCCA 2 cut(s) 598, 2408
MlyI GAGTC 5 cut(s) 875, 1285, 1598, 1904, 2742
MmeI TCCRAC 1 cut(s) 142
Mox20I TGGCCA 2 cut(s) 598, 2408
Mph1103I ATGCAT 2 cut(s) 2800, 3044
MroNI GCCGGC 1 cut(s) 3107
MroXI GAANNNNTTC 2 cut(s) 183, 2243
MscI TGGCCA 2 cut(s) 598, 2408
MslI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
Msp20I TGGCCA 2 cut(s) 598, 2408
MspA1I CMGCKG 2 cut(s) 2740, 2880
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 2 cut(s) 53, 3108
MspR9I CCNGG 5 cut(s) 176, 1989, 2249, 2659, 2813
MunI CAATTG 3 cut(s) 67, 567, 2699
Mva1269I GAATGC 2 cut(s) 306, 2798
MvaI CCWGG 5 cut(s) 176, 1989, 2249, 2659, 2813
MvnI CGCG 3 cut(s) 90, 144, 2948
NaeI GCCGGC 1 cut(s) 3109
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 2 cut(s) 1367, 1404
NdeII GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
NgoMIV GCCGGC 1 cut(s) 3107
NheI GCTAGC 1 cut(s) 2954
NlaIV GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 8 cut(s) 655, 733, 809, 895, 1108, 1430, 2044, 2051
NsiI ATGCAT 2 cut(s) 2800, 3044
NspI RCATGY 4 cut(s) 125, 610, 2146, 3019
OliI CACNNNNGTG 1 cut(s) 2222
PaeI GCATGC 1 cut(s) 3019
PaqCI CACCTGC 1 cut(s) 1039
PauI GCGCGC 1 cut(s) 142
PceI AGGCCT 2 cut(s) 59, 356
PciI ACATGT 2 cut(s) 121, 2142
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 2 cut(s) 306, 2798
PdiI GCCGGC 1 cut(s) 3109
PdmI GAANNNNTTC 2 cut(s) 183, 2243
PfeI GAWTC 4 cut(s) 1657, 1802, 1968, 2179
PflMI CCANNNNNTGG 2 cut(s) 827, 1422
PfoI TCCNGGA 2 cut(s) 174, 2247
PkrI GCNGC 7 cut(s) 89, 214, 341, 2023, 2739, 2742, 2979
PleI GAGTC 5 cut(s) 874, 1284, 1597, 1904, 2742
PpsI GAGTC 5 cut(s) 874, 1284, 1597, 1904, 2742
PscI ACATGT 2 cut(s) 121, 2142
PshBI ATTAAT 1 cut(s) 1301
Psp1406I AACGTT 1 cut(s) 2472
Psp6I CCWGG 5 cut(s) 174, 1987, 2247, 2657, 2811
PspEI GGTNACC 1 cut(s) 809
PspFI CCCAGC 3 cut(s) 1638, 2024, 2880
PspGI CCWGG 5 cut(s) 174, 1987, 2247, 2657, 2811
PspN4I GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2278, 2627
PspPI GGNCC 7 cut(s) 987, 2126, 2191, 2277, 2625, 2661, 3098
PstI CTGCAG 2 cut(s) 1050, 2155
PstNI CAGNNNCTG 3 cut(s) 254, 899, 1247
PsuI RGATCY 1 cut(s) 1975
PteI GCGCGC 1 cut(s) 142
PvuII CAGCTG 2 cut(s) 2740, 2880
RsaI GTAC 5 cut(s) 2037, 2392, 2429, 2644, 2964
RsaNI GTAC 5 cut(s) 2036, 2391, 2428, 2643, 2963
RseI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
SatI GCNGC 7 cut(s) 88, 213, 340, 2022, 2738, 2741, 2978
Sau3AI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 1975, 2195, 2487
Sau96I GGNCC 7 cut(s) 987, 2126, 2191, 2277, 2625, 2661, 3098
ScaI AGTACT 1 cut(s) 2392
SchI GAGTC 5 cut(s) 875, 1285, 1598, 1904, 2742
ScrFI CCNGG 5 cut(s) 176, 1989, 2249, 2659, 2813
SduI GDGCHC 2 cut(s) 1498, 1707
SfaNI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
SfcI CTRYAG 4 cut(s) 521, 1046, 2151, 2394
SinI GGWCC 4 cut(s) 987, 2126, 2277, 3098
SmiMI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
SmlI CTYRAG 2 cut(s) 61, 1661
SmoI CTYRAG 2 cut(s) 61, 1661
SphI GCATGC 1 cut(s) 3019
SseBI AGGCCT 2 cut(s) 59, 356
SsiI CCGC 4 cut(s) 88, 105, 469, 1335
SspMI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
StuI AGGCCT 2 cut(s) 59, 356
StyD4I CCNGG 5 cut(s) 174, 1987, 2247, 2657, 2811
StyI CCWWGG 3 cut(s) 357, 367, 2220
TaiI ACGT 4 cut(s) 33, 1473, 2422, 2475
TaqI TCGA 3 cut(s) 27, 187, 2721
TaqII GACCGA 1 cut(s) 1004
TatI WGTACW 2 cut(s) 2390, 2427
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 4 cut(s) 1657, 1802, 1968, 2179
TseFI GTSAC 8 cut(s) 655, 733, 809, 895, 1108, 1430, 2044, 2051
TseI GCWGC 6 cut(s) 212, 339, 2021, 2737, 2740, 2977
Tsp45I GTSAC 8 cut(s) 655, 733, 809, 895, 1108, 1430, 2044, 2051
TspGWI ACGGA 5 cut(s) 391, 491, 1388, 2717, 2852
Van91I CCANNNNNTGG 2 cut(s) 827, 1422
Vha464I CTTAAG 1 cut(s) 1661
VpaK11BI GGWCC 4 cut(s) 987, 2126, 2277, 3098
VspI ATTAAT 1 cut(s) 1301
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 7 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2230
XceI RCATGY 4 cut(s) 125, 610, 2146, 3019
XcmI CCANNNNNNNNNTGG 1 cut(s) 1402
XmnI GAANNNNTTC 2 cut(s) 183, 2243
XspI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
ZraI GACGTC 1 cut(s) 31
ZrmI AGTACT 1 cut(s) 2392
Zsp2I ATGCAT 2 cut(s) 2800, 3044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.