RLG00000023280

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002172
Physical Location & Seq
Reverse (-)
21091023 .. 21098376
7354 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023280

Sequence Viewer

Length: 3114 bp
ATGGAAAGCTACTTGCACGAGGACTTCAACATCCAGCCCAAGCACCGCTCGCCGGAGGCTTCAAGAAAATGGAAGAAAGCCGTCGGCCTCATCGTCAAGAACCGGGCCAGAAGGTTCCGACATGTCCCCGATCCTGACAAGCGCGCCGAGGCTGAGAGCAAGAAGAAGGAAATCCAGGAAAAAATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTACAATTCATTGAGGCTGGAGGTAGCCAACCAGCTACTGATCAGGTTGAATACAAACTGTCTGAAGAGGCCAAAGAAGTGGGTTTCAGCATTCACCCAGATGAACTTGCATCAATCATACGCAGCCATGATATTAAGGCCTTGGAAAGCCATGGTGGAGTTTATGGGATTTTAAAAAAGTTATCCGTCGTACCAGATGAAGGTGTCAGGGGTAGCAGTATGCCAACAAGGCAAAATGTTTTTGGCTTAAACCGCTATACGGAGAAGCCTCCCCGATCATTCTTGATGTTTGTATGGGAAGCTCTACAGGATTTAACTTTAATTATTCTTATGGTTTGTGCCGTGGTTTCAATTGGAGTGGGACTTGCTACTGACGGGTGGCCAGAAGGCATGTATGATGGGGTGGGAATTATACTCAGTATAGAATTAGTTGTTTTGGTCACTGCCATAAGTGATTACAGGCAGTCCATGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTATTCAGGTCACTAGGGATGGGAAAAGACAAAAAGTTTCCATTTATGATTTGCTGGTGGGAGATATTGTTCATTTGGCAGTGGGTGACCTAGTTCCAGCAGATGGACTTTTCATATCAGGATACAGCTTGTTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGAATGTAAATGAAGAAAAGCCTTTTCTTCTTTCGGGAACCAAAGTGCAAGATGGGTCAGGTAAAATGCTGGTAACAACAGTTGGCATGAGGACCGAATGGGGAAAGTTGATGGAAACTCTGAGTGAGGGAGGAGAAGATGAGACCCCACTCCAGGTGAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGGCTTTCGCTGTGGTGACATTTTTGGTTTTGACAGGAAGATATTTGGTGGAAAAAGCACTTACCAATGAGATCACAGATTGGTCTTCAACTGATGCTGTAACACTTTTGAACTACTTTGCCATTGCAGTAACTATAATTGTTGTTGCAGTTCCTGAAGGTTTACCATTGGCAGTAACACTGAGTCTTGCTTTTGCAATGAAGAAATTAATGAATGATAAGGCACTTGTGAGGCATCTCTCTGCCTGTGAGACAATGGGTTCTGCTAGTTGCATATGCACGGATAAAACAGGAACATTAACCACAAACCATATGGTAGTTACCAAGATATGGATAGGTGACAAATCCATAAATGTAAAAGGTAATGAAAGTAAAGATGTAGTAAAATCAGAAATTTCTGGAGCACTGGACATCCTATTGCAGGTTATATTTCAAAATACAAGTTCTGAGGTTATCAAGGATGAGGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCATTATTAGAATTTGGTTTGCTTTTGGGTGGCGATTTTGATGCCCAGCGCAGAGAGTTTAAGATTCTTAAGATGGAACCTTTCAATTCTGTTAGGAAGAAGATGTCTGTGCTCATCGCTCATTCTGATGGTGGGATAAGAGCTTTTTGCAAAGGTGCATCTGAAATAGTATTGGGAATGTGCAACAAAGTTATTGACTGTAATGGAGAATCTGTTGATCTCTCCCGAAAAGAGGCAAATAATATCACAGATGTCATAAATTCTTTTGCTTCTGAAGCATTAAGAACTCTCTGCTTGGCTTTTAAAGATATACATGACTCTTCCATCAACAATGACATCCCCAATGATGGCTATACATTGATAGCAGTTGTAGGAATCAAGGACCCTGTGCGTCCTGGGGTGAAGGAAGCAGTCCAAACTTGTTTGGCTGCTGGGATAACTGTACGAATGGTCACAGGTGACAATATAAATACTGCTAAAGCCATAGCTAGAGAATGTGGCATACTCACAGAGGATGGTTTGGCAATAGAAGGACCAGAATTTCGTAACATGTCTCCTGCAGATATGAAAGCTGTTATACCGAGGATTCAGGTGATGGCCCGATCCTTACCATTGGATAAGCACACCTTGGTGAAGAATTTGAGGAATACATTCCAGGAGGTAGTTGCAGTTACAGGTGATGGGACCAATGACGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGCACAGAGGTTGCTAAAGAAAGTGCTGATGTCATCGTATTGGATGACAATTTTAGTACTATAGTAAATGTGGCCAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTCCAGTTCCAATTAACGGTTAACGTTGTTGCTCTGATGATCAATTTTGTTTCTGCATGTGTATCAGGAGATGCTCCCCTTACTGCTGTGCAATTACTCTGGGTCAACATGATTATGGACACGCTTGGTGCCTTGGCACTGGCTACAGAGCCTCCAAATGAAGGACTGATGAAAAGGCCCCCTGTTTCAAGGGGTACAAGCTTCATCACCAGGTCCATGTGGAGGAATATCATTGGTCAGAGCATTTATCAACTGGCTGTTCTTGGAGTTCTCGATTTCCGTGGAACGCAGCTGCTAGGACTCACTGGTTCAGATGCAACTGATGTTCTTGACACTGTGATATTCAATGCATTCGTGTTCTGCCAGGTGTTTAATCAGATAAACAGCCGTGATATAGAGAAGATAAACATATTCCGTGGCATGTTTGACAGCTGGGTATTCCTAGGGGTCATGGTTTGCACAGTGGGCTTCCAAATTGTCCTAGTAGAGTTCTTGGGTGCGTTTGCTAGCACTGTACCACTGAGCTGGCAACTATGGTTACTCTGCATTATACTTGGATTGATTAGCATGCCTGTTGCAGTTGTCGTGAAATGCATACCCGTTGAAAGCAAAGTTAAACCCCCTGATGGCTATGAAGCTATCCCTGATGGTCCTGGCGCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1038

Amino Acids

112.98

Weight (kDa)

5.49

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 5 - 50 7.5e-13 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 122 - 189 6.3e-12 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 244 - 338 6.2e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 513 - 663 1.1e-32 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 650 - 770 1.1e-11 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1014 1.3e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1504
AccB1I GGYRCC 1 cut(s) 2576
AccB7I CCANNNNNTGG 2 cut(s) 827, 1422
AccBSI CCGCTC 1 cut(s) 48
AccII CGCG 1 cut(s) 144
AciI CCGC 2 cut(s) 46, 469
AclI AACGTT 1 cut(s) 2472
AclWI GGATC 2 cut(s) 125, 2190
AcoI YGGCCR 2 cut(s) 596, 2406
AcsI RAATTY 6 cut(s) 183, 1485, 1604, 1852, 2132, 2230
AcuI CTGAAG 3 cut(s) 300, 1269, 1887
AfaI GTAC 6 cut(s) 408, 2037, 2392, 2429, 2644, 2964
AflII CTTAAG 1 cut(s) 1661
AflIII ACRYGT 2 cut(s) 121, 2142
AjnI CCWGG 7 cut(s) 174, 1047, 1987, 2247, 2657, 2811, 3100
AleI CACNNNNGTG 1 cut(s) 2222
AloI GAACNNNNNNTCC 2 cut(s) 777, 809
Alw21I GWGCWC 2 cut(s) 1498, 1707
Alw26I GTCTC 3 cut(s) 1031, 1337, 2151
AlwI GGATC 2 cut(s) 125, 2190
AlwNI CAGNNNCTG 2 cut(s) 254, 1247
AoxI GGCC 8 cut(s) 85, 105, 285, 354, 596, 2190, 2406, 2624
ApeKI GCWGC 5 cut(s) 212, 339, 2021, 2737, 2740
ApoI RAATTY 6 cut(s) 183, 1485, 1604, 1852, 2132, 2230
ArsI GACNNNNNNTTYG 4 cut(s) 1555, 1587, 2118, 2150
AseI ATTAAT 1 cut(s) 1301
Asp700I GAANNNNTTC 2 cut(s) 183, 2243
AspA2I CCTAGG 1 cut(s) 2890
AspLEI GCGC 4 cut(s) 144, 146, 1644, 3107
AspS9I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
AsuC2I CCSGG 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 2954
AvaII GGWCC 6 cut(s) 987, 1975, 2126, 2277, 2661, 3098
AvrII CCTAGG 1 cut(s) 2890
BalI TGGCCA 2 cut(s) 598, 2408
BanI GGYRCC 1 cut(s) 2576
BauI CACGAG 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 2 cut(s) 1498, 1707
BbvI GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
BceAI ACGGC 3 cut(s) 65, 542, 2820
BcgI CGANNNNNNTGC 2 cut(s) 1616, 1650
BciT130I CCWGG 7 cut(s) 176, 1049, 1989, 2249, 2659, 2813, 3102
BciVI GTATCC 1 cut(s) 839
BclI TGATCA 2 cut(s) 256, 2487
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 3 cut(s) 1031, 1337, 2151
BfaI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
BfmI CTRYAG 4 cut(s) 521, 2151, 2394, 2592
BfoI RGCGCY 1 cut(s) 3108
BfrI CTTAAG 1 cut(s) 1661
BfuAI ACCTGC 1 cut(s) 1504
BfuI GTATCC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 445
BisI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
BlnI CCTAGG 1 cut(s) 2890
BlsI GCNGC 5 cut(s) 214, 341, 2023, 2739, 2742
BmcAI AGTACT 1 cut(s) 2392
Bme1390I CCNGG 8 cut(s) 104, 176, 1049, 1989, 2249, 2659, 2813, 3102
Bme18I GGWCC 6 cut(s) 987, 1975, 2126, 2277, 2661, 3098
BmgT120I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
BmiI GGNNCC 7 cut(s) 116, 934, 1671, 1977, 2278, 2578, 2627
BmrFI CCNGG 8 cut(s) 104, 176, 1049, 1989, 2249, 2659, 2813, 3102
BmsI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
BmtI GCTAGC 1 cut(s) 2958
BpiI GAAGAC 1 cut(s) 1170
BpmI CTGGAG 3 cut(s) 255, 1031, 1512
BpuMI CCSGG 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 1031
BsaXI ACNNNNNCTCC 6 cut(s) 777, 807, 1791, 1821, 2584, 2614
Bse1I ACTGG 6 cut(s) 893, 1503, 2452, 2592, 2706, 2758
Bse3DI GCAATG 2 cut(s) 1215, 1296
BseBI CCWGG 7 cut(s) 176, 1049, 1989, 2249, 2659, 2813, 3102
BseGI GGATG 7 cut(s) 30, 748, 1503, 1558, 1929, 2113, 2383
BseMI GCAATG 2 cut(s) 1215, 1296
BseMII CTCAG 6 cut(s) 144, 646, 1007, 1265, 1530, 2960
BseNI ACTGG 6 cut(s) 893, 1503, 2452, 2592, 2706, 2758
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 1041
BseXI GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
BseYI CCCAGC 3 cut(s) 1638, 2024, 2880
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 8 cut(s) 87, 107, 287, 356, 598, 2192, 2408, 2626
BshNI GGYRCC 1 cut(s) 2576
BsiHKAI GWGCWC 2 cut(s) 1498, 1707
BsiSI CCGG 2 cut(s) 53, 103
BslFI GGGAC 4 cut(s) 110, 591, 2290, 2430
BsmAI GTCTC 3 cut(s) 1031, 1337, 2151
BsmFI GGGAC 4 cut(s) 110, 591, 2290, 2430
BsmI GAATGC 2 cut(s) 306, 2798
BsnI GGCC 8 cut(s) 87, 107, 287, 356, 598, 2192, 2408, 2626
Bso31I GGTCTC 1 cut(s) 1031
Bsp1286I GDGCHC 2 cut(s) 1498, 1707
Bsp1407I TGTACA 1 cut(s) 2427
Bsp143I GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 2 cut(s) 46, 469
BspANI GGCC 8 cut(s) 87, 107, 287, 356, 598, 2192, 2408, 2626
BspCNI CTCAG 6 cut(s) 145, 645, 1008, 1266, 1531, 2961
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 7 cut(s) 116, 934, 1671, 1977, 2278, 2578, 2627
BspMAI CTGCAG 1 cut(s) 2155
BspMI ACCTGC 1 cut(s) 1504
BspOI GCTAGC 1 cut(s) 2958
BspPI GGATC 2 cut(s) 125, 2190
BspT107I GGYRCC 1 cut(s) 2576
BspTI CTTAAG 1 cut(s) 1661
BspTNI GGTCTC 1 cut(s) 1031
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 1215, 1296
BsrGI TGTACA 1 cut(s) 2427
BsrI ACTGG 6 cut(s) 893, 1503, 2452, 2592, 2706, 2758
BssHII GCGCGC 1 cut(s) 142
BssMI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
BssSI CACGAG 1 cut(s) 17
BssT1I CCWWGG 5 cut(s) 357, 367, 2220, 2580, 2890
Bst2BI CACGAG 1 cut(s) 17
Bst2UI CCWGG 7 cut(s) 176, 1049, 1989, 2249, 2659, 2813, 3102
Bst4CI ACNGT 8 cut(s) 276, 976, 1793, 2035, 2467, 2785, 2911, 2962
Bst6I CTCTTC 2 cut(s) 276, 1918
BstAFI CTTAAG 1 cut(s) 1661
BstAPI GCANNNNNTGC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 2427
BstC8I GCNNGC 6 cut(s) 50, 144, 2335, 2956, 2975, 3017
BstDEI CTNAG 6 cut(s) 153, 632, 1016, 1274, 1539, 2969
BstDSI CCRYGG 4 cut(s) 367, 558, 2728, 2863
BstEII GGTNACC 1 cut(s) 809
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 7 cut(s) 30, 748, 1503, 1558, 1929, 2113, 2383
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 3108
BstHHI GCGC 4 cut(s) 144, 146, 1644, 3107
BstKTI GATC 7 cut(s) 133, 259, 494, 1167, 1813, 2198, 2490
BstMAI GTCTC 3 cut(s) 1031, 1337, 2151
BstMBI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
BstMWI GCNNNNNNNGC 7 cut(s) 49, 445, 468, 685, 1868, 2334, 2913
BstNI CCWGG 7 cut(s) 176, 1049, 1989, 2249, 2659, 2813, 3102
BstNSI RCATGY 6 cut(s) 125, 610, 2146, 2508, 2872, 3019
BstPI GGTNACC 1 cut(s) 809
BstSCI CCNGG 8 cut(s) 102, 174, 1047, 1987, 2247, 2657, 2811, 3100
BstSFI CTRYAG 4 cut(s) 521, 2151, 2394, 2592
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
BstV2I GAAGAC 1 cut(s) 1170
BstXI CCANNNNNNTGG 2 cut(s) 295, 2973
BsuI GTATCC 1 cut(s) 839
BsuRI GGCC 8 cut(s) 87, 107, 287, 356, 598, 2192, 2408, 2626
BtgI CCRYGG 4 cut(s) 367, 558, 2728, 2863
BtgZI GCGATG 1 cut(s) 1693
BtsCI GGATG 7 cut(s) 30, 748, 1503, 1558, 1929, 2113, 2383
BtsI GCAGTG 2 cut(s) 657, 810
BveI ACCTGC 1 cut(s) 1504
Cac8I GCNNGC 6 cut(s) 50, 144, 2335, 2956, 2975, 3017
CaiI CAGNNNCTG 2 cut(s) 254, 1247
CfoI GCGC 4 cut(s) 144, 146, 1644, 3107
Cfr13I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
CseI GACGC 2 cut(s) 1973, 2294
CsiI ACCWGGT 1 cut(s) 2657
Csp6I GTAC 6 cut(s) 407, 2036, 2391, 2428, 2643, 2963
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviQI GTAC 6 cut(s) 407, 2036, 2391, 2428, 2643, 2963
DdeI CTNAG 6 cut(s) 153, 632, 1016, 1274, 1539, 2969
DpnI GATC 7 cut(s) 132, 258, 493, 1166, 1812, 2197, 2489
DpnII GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
DraI TTTAAA 2 cut(s) 390, 1897
EaeI YGGCCR 2 cut(s) 596, 2406
Eam1104I CTCTTC 2 cut(s) 276, 1918
EarI CTCTTC 2 cut(s) 276, 1918
Eco130I CCWWGG 5 cut(s) 357, 367, 2220, 2580, 2890
Eco147I AGGCCT 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 6 cut(s) 987, 1975, 2126, 2277, 2661, 3098
Eco57I CTGAAG 3 cut(s) 300, 1269, 1887
Eco91I GGTNACC 1 cut(s) 809
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 2 cut(s) 1975, 2625
EcoO65I GGTNACC 1 cut(s) 809
EcoRII CCWGG 7 cut(s) 174, 1047, 1987, 2247, 2657, 2811, 3100
EcoT14I CCWWGG 5 cut(s) 357, 367, 2220, 2580, 2890
EcoT22I ATGCAT 2 cut(s) 2800, 3044
ErhI CCWWGG 5 cut(s) 357, 367, 2220, 2580, 2890
FalI AAGNNNNNCTT 2 cut(s) 2204, 2236
FaqI GGGAC 4 cut(s) 110, 591, 2290, 2430
FauNDI CATATG 2 cut(s) 1367, 1404
FbaI TGATCA 2 cut(s) 256, 2487
Fnu4HI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
FokI GGATG 7 cut(s) 17, 755, 1490, 1565, 1916, 2120, 2390
Fsp4HI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
FspBI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
GlaI GCGC 4 cut(s) 143, 145, 1643, 3106
GluI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
GsaI CCCAGC 3 cut(s) 1642, 2028, 2884
GsuI CTGGAG 3 cut(s) 255, 1031, 1512
HaeII RGCGCY 1 cut(s) 3108
HaeIII GGCC 8 cut(s) 87, 107, 287, 356, 598, 2192, 2408, 2626
HapII CCGG 2 cut(s) 53, 103
HgaI GACGC 2 cut(s) 1973, 2294
HhaI GCGC 4 cut(s) 144, 146, 1644, 3107
Hin6I GCGC 4 cut(s) 142, 144, 1642, 3105
HinP1I GCGC 4 cut(s) 142, 144, 1642, 3105
HincII GTYRAC 2 cut(s) 2470, 2554
HindII GTYRAC 2 cut(s) 2470, 2554
HindIII AAGCTT 2 cut(s) 871, 2647
HinfI GANTC 9 cut(s) 866, 1276, 1589, 1657, 1802, 1910, 1968, 2179, 2748
HpaI GTTAAC 1 cut(s) 2470
HpaII CCGG 2 cut(s) 53, 103
Hpy166II GTNNAC 4 cut(s) 1256, 2428, 2470, 2554
Hpy8I GTNNAC 4 cut(s) 1256, 2428, 2470, 2554
Hpy99I CGWCG 2 cut(s) 86, 407
HpyAV CCTTC 8 cut(s) 105, 160, 410, 596, 1244, 1990, 2117, 2603
HpyCH4III ACNGT 8 cut(s) 276, 976, 1793, 2035, 2467, 2785, 2911, 2962
HpyCH4IV ACGT 2 cut(s) 2419, 2472
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 445, 468, 685, 1868, 2334, 2913
HpyF3I CTNAG 6 cut(s) 153, 632, 1016, 1274, 1539, 2969
HpySE526I ACGT 2 cut(s) 2419, 2472
HspAI GCGC 4 cut(s) 142, 144, 1642, 3105
Ksp22I TGATCA 2 cut(s) 256, 2487
KspAI GTTAAC 1 cut(s) 2470
Kzo9I GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
LmnI GCTCC 3 cut(s) 1493, 2293, 2527
Lsp1109I GCAGC 5 cut(s) 224, 351, 2008, 2727, 2749
LweI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
MabI ACCWGGT 1 cut(s) 2657
MaeI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
MaeII ACGT 2 cut(s) 2419, 2472
MalI GATC 7 cut(s) 132, 258, 493, 1166, 1812, 2197, 2489
MbiI CCGCTC 1 cut(s) 48
MboI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
MfeI CAATTG 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 1498, 1707
MlsI TGGCCA 2 cut(s) 598, 2408
MluNI TGGCCA 2 cut(s) 598, 2408
MlyI GAGTC 5 cut(s) 875, 1285, 1598, 1904, 2742
MmeI TCCRAC 1 cut(s) 142
Mox20I TGGCCA 2 cut(s) 598, 2408
Mph1103I ATGCAT 2 cut(s) 2800, 3044
MroXI GAANNNNTTC 2 cut(s) 183, 2243
MscI TGGCCA 2 cut(s) 598, 2408
MslI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
Msp20I TGGCCA 2 cut(s) 598, 2408
MspA1I CMGCKG 2 cut(s) 2740, 2880
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 2 cut(s) 53, 103
MspR9I CCNGG 8 cut(s) 104, 176, 1049, 1989, 2249, 2659, 2813, 3102
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 2 cut(s) 306, 2798
MvaI CCWGG 7 cut(s) 176, 1049, 1989, 2249, 2659, 2813, 3102
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 7 cut(s) 49, 445, 468, 685, 1868, 2334, 2913
NciI CCSGG 1 cut(s) 104
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 2 cut(s) 1367, 1404
NdeII GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
NheI GCTAGC 1 cut(s) 2954
NlaIV GGNNCC 7 cut(s) 116, 934, 1671, 1977, 2278, 2578, 2627
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
NsiI ATGCAT 2 cut(s) 2800, 3044
NspI RCATGY 6 cut(s) 125, 610, 2146, 2508, 2872, 3019
OliI CACNNNNGTG 1 cut(s) 2222
PaeI GCATGC 1 cut(s) 3019
PauI GCGCGC 1 cut(s) 142
PceI AGGCCT 1 cut(s) 356
PciI ACATGT 2 cut(s) 121, 2142
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 2 cut(s) 306, 2798
PdmI GAANNNNTTC 2 cut(s) 183, 2243
PfeI GAWTC 4 cut(s) 1657, 1802, 1968, 2179
PflMI CCANNNNNTGG 2 cut(s) 827, 1422
PfoI TCCNGGA 2 cut(s) 174, 2247
PkrI GCNGC 5 cut(s) 214, 341, 2023, 2739, 2742
PleI GAGTC 5 cut(s) 874, 1284, 1597, 1904, 2742
PpsI GAGTC 5 cut(s) 874, 1284, 1597, 1904, 2742
PpuMI RGGWCCY 1 cut(s) 1975
PscI ACATGT 2 cut(s) 121, 2142
PshBI ATTAAT 1 cut(s) 1301
Psp1406I AACGTT 1 cut(s) 2472
Psp5II RGGWCCY 1 cut(s) 1975
Psp6I CCWGG 7 cut(s) 174, 1047, 1987, 2247, 2657, 2811, 3100
PspEI GGTNACC 1 cut(s) 809
PspFI CCCAGC 3 cut(s) 1638, 2024, 2880
PspGI CCWGG 7 cut(s) 174, 1047, 1987, 2247, 2657, 2811, 3100
PspN4I GGNNCC 7 cut(s) 116, 934, 1671, 1977, 2278, 2578, 2627
PspPI GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
PspPPI RGGWCCY 1 cut(s) 1975
PstI CTGCAG 1 cut(s) 2155
PstNI CAGNNNCTG 2 cut(s) 254, 1247
PteI GCGCGC 1 cut(s) 142
PvuII CAGCTG 2 cut(s) 2740, 2880
RsaI GTAC 6 cut(s) 408, 2037, 2392, 2429, 2644, 2964
RsaNI GTAC 6 cut(s) 407, 2036, 2391, 2428, 2643, 2963
RseI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
SatI GCNGC 5 cut(s) 213, 340, 2022, 2738, 2741
Sau3AI GATC 7 cut(s) 130, 256, 491, 1164, 1810, 2195, 2487
Sau96I GGNCC 9 cut(s) 105, 987, 1975, 2126, 2191, 2277, 2625, 2661, 3098
ScaI AGTACT 1 cut(s) 2392
SchI GAGTC 5 cut(s) 875, 1285, 1598, 1904, 2742
ScrFI CCNGG 8 cut(s) 104, 176, 1049, 1989, 2249, 2659, 2813, 3102
SduI GDGCHC 2 cut(s) 1498, 1707
SexAI ACCWGGT 1 cut(s) 2657
SfaNI GCATC 7 cut(s) 335, 1177, 1336, 1624, 1760, 2509, 2752
SfcI CTRYAG 4 cut(s) 521, 2151, 2394, 2592
SinI GGWCC 6 cut(s) 987, 1975, 2126, 2277, 2661, 3098
SmiMI CAYNNNNRTG 4 cut(s) 315, 1719, 2222, 2561
SmlI CTYRAG 1 cut(s) 1661
SmoI CTYRAG 1 cut(s) 1661
SphI GCATGC 1 cut(s) 3019
SseBI AGGCCT 1 cut(s) 356
SsiI CCGC 2 cut(s) 46, 469
SspMI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
StuI AGGCCT 1 cut(s) 356
StyD4I CCNGG 8 cut(s) 102, 174, 1047, 1987, 2247, 2657, 2811, 3100
StyI CCWWGG 5 cut(s) 357, 367, 2220, 2580, 2890
TaaI ACNGT 8 cut(s) 276, 976, 1793, 2035, 2467, 2785, 2911, 2962
TaiI ACGT 2 cut(s) 2422, 2475
TaqI TCGA 2 cut(s) 187, 2721
TaqII GACCGA 1 cut(s) 1004
TatI WGTACW 2 cut(s) 2390, 2427
TfiI GAWTC 4 cut(s) 1657, 1802, 1968, 2179
TseFI GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
TseI GCWGC 5 cut(s) 212, 339, 2021, 2737, 2740
Tsp45I GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
TspGWI ACGGA 5 cut(s) 391, 491, 1388, 2717, 2852
Van91I CCANNNNNTGG 2 cut(s) 827, 1422
Vha464I CTTAAG 1 cut(s) 1661
VpaK11BI GGWCC 6 cut(s) 987, 1975, 2126, 2277, 2661, 3098
VspI ATTAAT 1 cut(s) 1301
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 6 cut(s) 183, 1485, 1604, 1852, 2132, 2230
XceI RCATGY 6 cut(s) 125, 610, 2146, 2508, 2872, 3019
XcmI CCANNNNNNNNNTGG 1 cut(s) 1402
XmaJI CCTAGG 1 cut(s) 2890
XmnI GAANNNNTTC 2 cut(s) 183, 2243
XspI CTAG 8 cut(s) 738, 815, 1359, 2082, 2744, 2891, 2930, 2955
ZrmI AGTACT 1 cut(s) 2392
Zsp2I ATGCAT 2 cut(s) 2800, 3044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.