FvH4_6g24440

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002172
Physical Location & Seq
Forward (+)
18536560 .. 18544235
7676 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24440.t3

Sequence Viewer

Length: 3129 bp
ATGGAGAGCTACCTGCACAAGGACTTCGACGTGCAGCCCAAGCACCTCTCGGCGGAGGCTTCCAGCAACTGGAGGAGGGCCGTCGGCCGCGTCGTCAAGAACCCCGCCAGACGCTTCCGTCATGTCGCCGATCTCGCCAAGCGCGCCGAGGCCGAGAAAAAGAAGAAGCAAATCCAGGAAAAAATTAGAGTTGCTCTTTATGTTCAAAAAGCAGCTATGCATTTCATTGAGGCTGGAGATAGTCAGCCAGCTACAGAAAAGCGTGATCAAGTTGAATACAAACTGTCTGAAGAGGCCGAAAAAGAAGGTTTCAGCATTCACCCAGATGAACTTGCATCAATCATACGTAGCCATGATACCAAGGTCTTGGAAATCCATGGTGGAGTTGATGGGATTTTAAATAAATTAGCTGTCACACCAGATGAAGGTGTAAAGGGTAGTAGTATACCAACAAGGCAAAATGTTTATGGTTTAAATCGCTATACAGAGAAACCTCCCCGATCATTCTTGAGGTTTGTATGGGAAGCACTACAGGATTTAACTTTAATTATTCTTATGGTTTGTGCCGTGGTTTCAATTGGAGTGGGAATTGGTACTGAAGGGTGGCCAGAAGGCATGTATGATGGGGTGGGAATCTTACTCAGTATAGTTTTGGTTGTTTTGGTTACTGCCATTAGTGACTACAGGCAGTCCATGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTATTCAGGTCACTAGGGATGGGAAAAGACAAAAAGTTTCCATTTATGACTTGCTGGTTGGAGATATTGTTCATTTGGCAGTAGGAGACCAAGTTCCGGCAGATGGACTTTTCATATCAGGATACAGTTTGTTGATTGATGAGTCAAGCTTGACAGGTGAGAGTGAGCCGATGAATGTATATGAAAAAAAGCCTTTTCTTCTTTCAGGAACCAAAGTGCAAGATGGGTCTGGTAAAATGCTGGTAACAACAGTTGGTATGAGGACCGAATGGGGAAAATTGATGGAAACTTTGAGTGAGGGAGGCGAAGATGAGACCCCACTACAGGTGAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGGCTTTCGCTGTGGTGACATTTATGGTCTTGACAGTGAGATTTTTGGTGGAAAAAGCACTTAGCAATGAGATCACGGATTGGTCTTCAACTGATGCAATGACACTTTTGAACTACTTTGCCATCGCAGTAACTATAATTGTTGTTGCAGTTCCTGAAGGATTACCATTGGCAGTAACACTGAGTCTTGCGTTTGCAATGAAGAAATTAATGAATGATAAGGCACTTGTGAGGCATCTCTCAGCCTGCGAGACAATGGGTTCTGCTAGTTGCATTTGCACGGATAAAACAGGAACATTAACCACAAACCATATGGTAGTTACCAAGATATGGATAGGTGAAAGATCTGTAGATGTAAGCGGTAATAAAAGTACAGACATAGTAAAATCAGAAATTTCTGGAGCACTGGACATCCTTTTGCAGGTTATATTTCAAAATACAAGCTCTGAGGTCATCAAGGATGAGGGAAAGACCTCCATATTGGGAACACCAACAGAGTCAGCATTATTAGAATTTGGTTTACTTTTGGGTGGTGATTTTGATGCCCAGCGCAGAGAGTTTAAGATTATTAAGATGGAACCTTTCAGTTCTGTTAGGAAGAAAATGTCTGTGCTCATTGCTCATCCTCATGGTGGGGTAAGAGCTTTTTGCAAAGGTGCATCTGAAATAGTATTGGGAATGTGCAACAAGGTTATTGACTGTAATGGAGAAACTGTTAATCTCTCCCGAGAAGAGGCAAATAATATCACAGATGTCATAAATTCTTTTGCTTGTGAAGCACTAAGAACTCTTTGCTTGGCTTTCAAAGATATCGATGAATCTTCCATCAACAATGACATCCCTGATGATGGCTATACATTAATTGCAGTTGTTGGAATCAAGGACCCTGTGCGTCCAGGGGTGAAGGAAGCAGTCCAAACTTGTTTGGCTGCTGGGATAACTGTACGAATGGTCACCGGTGACAATATAAATACTGCTAAGGCCATAGCTAGAGAATGTGGCATACTCACAGAGGATGGTCTGGCAATAGAGGGACCAGAATTTCGTAACTTGTCTCCTGCAGAGATGAATGCTGTCATACCGAAGATTCAGGTGATGGCTCGGTCCTTACCATTGGACAAGCACACCTTGGTGAAGAATTTGAGGAATACATTCCGGGAGGTAGTTGCAGTTACAGGTGATGGGACCAATGATGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGAATAGCAGGCACAGAGGTTGCAAAAGAAAGTGCTGATGTCATCATATTGGATGACAATTTTAGTACTATAGTGAATGTGGCCAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTGCAGTTCCAGTTGACGGTTAACGTTGTTGCTCTGATGATCAACTTTGTTTCTGCATGTGTATCAGGAGATGCTCCCCTTACTGCTGTGCAATTACTCTGGGTCAACATGATTATGGACACTCTTGGGGCTTTGGCTTTGGCTACTGAGCCTCCAAATGACGGACTGATGAAAAGGCCCCCTGTTTCAAGGGGTACAAGCTTCATCACCAAGACCATGTGGAGGAATATCATTGGTCAGAGCATTTATCAACTGGCTGTTCTTGGAGTTCTCGATTTCCGTGGGACACAGCTGCTAGGACTTACTGGTTCAGATGCAACCGATATTCTTAATACTGTGATATTCAATGCATTCGTGTTCTGCCAGGTGTTTAATGAGATAAACAGCCGTGATATAGAGAAGATAAACATATTCCGTGGCATGTTTGACAGCTGGGTATTCCTGGGGGTTATGGTTTGCACAGTGGCCTTCCAAATTGTCTTAGTAGAGTTCTTGGGCGCATTTGCTAGCACTGTACCATTGAGCTGGCAACTGTGGTTACTCTGCATTATAATTGGATCGATTAGCATGCCTGTTGCAGTTGTCTTGAAATGCATACCCGTTGAAAGCAAAGTTAAACCCCCTGAGGGCTATGAAGCTATTCCTGATGGTCCTCCAGCTGGCCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1043

Amino Acids

113.65

Weight (kDa)

5.49

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 62 - 156 1.7e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 331 - 481 6e-32 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 466 - 588 4.5e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 659 - 832 9.2e-43 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3008
AasI GACNNNNNNGTC 1 cut(s) 117
Acc36I ACCTGC 2 cut(s) 21, 1513
AccB7I CCANNNNNTGG 2 cut(s) 69, 1431
AccI GTMKAC 1 cut(s) 445
AccII CGCG 2 cut(s) 90, 144
AciI CCGC 4 cut(s) 53, 88, 105, 1461
AclI AACGTT 1 cut(s) 2481
AclWI GGATC 1 cut(s) 3022
AcoI YGGCCR 3 cut(s) 85, 605, 2415
AcsI RAATTY 5 cut(s) 1494, 1613, 1861, 2141, 2239
AcuI CTGAAG 3 cut(s) 309, 618, 1278
AfaI GTAC 7 cut(s) 595, 1474, 2046, 2401, 2438, 2653, 2973
AgeI ACCGGT 1 cut(s) 2057
AjiI CACGTC 1 cut(s) 31
AjnI CCWGG 4 cut(s) 174, 1996, 2820, 2898
AleI CACNNNNGTG 1 cut(s) 2231
AloI GAACNNNNNNTCC 4 cut(s) 786, 810, 818, 842
Alw21I GWGCWC 2 cut(s) 1507, 1716
Alw26I GTCTC 4 cut(s) 813, 1040, 1346, 2160
AlwI GGATC 1 cut(s) 3022
AlwNI CAGNNNCTG 2 cut(s) 69, 1256
Ama87I CYCGRG 1 cut(s) 1827
ApeKI GCWGC 4 cut(s) 34, 212, 2030, 2749
ApoI RAATTY 5 cut(s) 1494, 1613, 1861, 2141, 2239
ArsI GACNNNNNNTTYG 2 cut(s) 2127, 2159
AseI ATTAAT 2 cut(s) 1310, 1961
AsiGI ACCGGT 1 cut(s) 2057
Asp700I GAANNNNTTC 2 cut(s) 2252, 3096
AspLEI GCGC 4 cut(s) 144, 146, 1653, 2957
AspS9I GGNCC 8 cut(s) 78, 996, 1984, 2135, 2205, 2286, 2634, 3107
AsuC2I CCSGG 1 cut(s) 2258
AsuNHI GCTAGC 1 cut(s) 2963
AvaI CYCGRG 1 cut(s) 1827
AvaII GGWCC 6 cut(s) 996, 1984, 2135, 2205, 2286, 3107
AxyI CCTNAGG 1 cut(s) 3081
BaeI ACNNNNGTAYC 2 cut(s) 348, 381
BalI TGGCCA 2 cut(s) 607, 2417
BbsI GAAGAC 1 cut(s) 1179
Bbv12I GWGCWC 2 cut(s) 1507, 1716
BbvI GCAGC 4 cut(s) 46, 224, 2017, 2736
BceAI ACGGC 3 cut(s) 65, 551, 2829
BcgI CGANNNNNNTGC 2 cut(s) 3007, 3041
BciT130I CCWGG 4 cut(s) 176, 1998, 2822, 2900
BciVI GTATCC 1 cut(s) 848
BclI TGATCA 2 cut(s) 265, 2496
BcnI CCSGG 1 cut(s) 2258
BcoDI GTCTC 4 cut(s) 813, 1040, 1346, 2160
BfaI CTAG 5 cut(s) 747, 1368, 2091, 2753, 2964
BfmI CTRYAG 7 cut(s) 252, 530, 682, 1055, 1449, 2160, 2403
BfuAI ACCTGC 2 cut(s) 21, 1513
BfuI GTATCC 1 cut(s) 848
BglII AGATCT 1 cut(s) 1445
BisI GCNGC 5 cut(s) 35, 88, 213, 2031, 2750
BlsI GCNGC 5 cut(s) 36, 89, 214, 2032, 2751
BmcAI AGTACT 1 cut(s) 2401
Bme1390I CCNGG 5 cut(s) 176, 1998, 2258, 2822, 2900
Bme18I GGWCC 6 cut(s) 996, 1984, 2135, 2205, 2286, 3107
BmeT110I CYCGRG 1 cut(s) 1827
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 8 cut(s) 78, 996, 1984, 2135, 2205, 2286, 2634, 3107
BmiI GGNNCC 6 cut(s) 943, 1680, 1986, 2136, 2287, 2636
BmrFI CCNGG 5 cut(s) 176, 1998, 2258, 2822, 2900
BmsI GCATC 8 cut(s) 344, 1186, 1345, 1633, 1769, 2284, 2518, 2761
BmtI GCTAGC 1 cut(s) 2967
BpiI GAAGAC 1 cut(s) 1179
BpmI CTGGAG 4 cut(s) 91, 255, 1521, 3096
Bpu10I CCTNAGC 1 cut(s) 2079
BpuEI CTTGAG 1 cut(s) 529
BpuMI CCSGG 1 cut(s) 2258
Bsa29I ATCGAT 2 cut(s) 1914, 3017
BsaAI YACGTR 1 cut(s) 347
BsaI GGTCTC 2 cut(s) 813, 1040
BsaJI CCNNGG 9 cut(s) 147, 360, 376, 567, 1997, 2229, 2737, 2872, 2899
BsaWI WCCGGW 1 cut(s) 2057
BsaXI ACNNNNNCTCC 8 cut(s) 786, 810, 816, 840, 1800, 1830, 2593, 2623
Bse118I RCCGGY 1 cut(s) 2057
Bse1I ACTGG 5 cut(s) 74, 1512, 2467, 2715, 2767
Bse21I CCTNAGG 1 cut(s) 3081
Bse3DI GCAATG 4 cut(s) 1174, 1206, 1305, 1716
BseBI CCWGG 4 cut(s) 176, 1998, 2822, 2900
BseCI ATCGAT 2 cut(s) 1914, 3017
BseDI CCNNGG 9 cut(s) 147, 360, 376, 567, 1997, 2229, 2737, 2872, 2899
BseGI GGATG 7 cut(s) 757, 1512, 1567, 1723, 1938, 2122, 2392
BseMI GCAATG 4 cut(s) 1174, 1206, 1305, 1716
BseMII CTCAG 6 cut(s) 655, 1274, 1356, 1539, 2595, 3072
BseNI ACTGG 5 cut(s) 74, 1512, 2467, 2715, 2767
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 88
BseX3I CGGCCG 1 cut(s) 85
BseXI GCAGC 4 cut(s) 46, 224, 2017, 2736
BseYI CCCAGC 3 cut(s) 1647, 2033, 2889
BsgI GTGCAG 2 cut(s) 53, 2480
Bsh1236I CGCG 2 cut(s) 90, 144
Bsh1285I CGRYCG 1 cut(s) 88
BshTI ACCGGT 1 cut(s) 2057
BshVI ATCGAT 2 cut(s) 1914, 3017
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 1507, 1716
BsiHKCI CYCGRG 1 cut(s) 1827
BsiSI CCGG 3 cut(s) 830, 2058, 2257
BslFI GGGAC 4 cut(s) 2148, 2299, 2439, 2755
BsmAI GTCTC 4 cut(s) 813, 1040, 1346, 2160
BsmFI GGGAC 4 cut(s) 2148, 2299, 2439, 2755
BsmI GAATGC 3 cut(s) 315, 2176, 2807
Bso31I GGTCTC 2 cut(s) 813, 1040
BsoBI CYCGRG 1 cut(s) 1827
Bsp1286I GDGCHC 2 cut(s) 1507, 1716
Bsp1407I TGTACA 1 cut(s) 2436
Bsp143I GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
Bsp19I CCATGG 1 cut(s) 376
BspACI CCGC 4 cut(s) 53, 88, 105, 1461
BspCNI CTCAG 6 cut(s) 654, 1275, 1355, 1540, 2596, 3073
BspDI ATCGAT 2 cut(s) 1914, 3017
BspFNI CGCG 2 cut(s) 90, 144
BspLI GGNNCC 6 cut(s) 943, 1680, 1986, 2136, 2287, 2636
BspMAI CTGCAG 1 cut(s) 2164
BspMI ACCTGC 2 cut(s) 21, 1513
BspOI GCTAGC 1 cut(s) 2967
BspPI GGATC 1 cut(s) 3022
BspTNI GGTCTC 2 cut(s) 813, 1040
BsrDI GCAATG 4 cut(s) 1174, 1206, 1305, 1716
BsrFI RCCGGY 1 cut(s) 2057
BsrGI TGTACA 1 cut(s) 2436
BsrI ACTGG 5 cut(s) 74, 1512, 2467, 2715, 2767
BssAI RCCGGY 1 cut(s) 2057
BssECI CCNNGG 9 cut(s) 147, 360, 376, 567, 1997, 2229, 2737, 2872, 2899
BssHII GCGCGC 1 cut(s) 142
BssMI GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
BssNAI GTATAC 1 cut(s) 446
BssT1I CCWWGG 3 cut(s) 360, 376, 2229
Bst1107I GTATAC 1 cut(s) 446
Bst2UI CCWGG 4 cut(s) 176, 1998, 2822, 2900
Bst6I CTCTTC 2 cut(s) 285, 1827
BstAPI GCANNNNNTGC 1 cut(s) 694
BstAUI TGTACA 1 cut(s) 2436
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 8 cut(s) 144, 249, 1348, 2344, 2965, 2984, 3026, 3118
BstDSI CCRYGG 4 cut(s) 376, 567, 2737, 2872
BstEII GGTNACC 1 cut(s) 2053
BstENI CCTNNNNNAGG 2 cut(s) 17, 1520
BstF5I GGATG 7 cut(s) 757, 1512, 1567, 1723, 1938, 2122, 2392
BstFNI CGCG 2 cut(s) 90, 144
BstHHI GCGC 4 cut(s) 144, 146, 1653, 2957
BstKTI GATC 7 cut(s) 133, 268, 503, 1176, 1448, 2499, 3017
BstMAI GTCTC 4 cut(s) 813, 1040, 1346, 2160
BstMBI GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 5 cut(s) 40, 134, 143, 694, 1877
BstNI CCWGG 4 cut(s) 176, 1998, 2822, 2900
BstNSI RCATGY 4 cut(s) 619, 2517, 2881, 3028
BstPI GGTNACC 1 cut(s) 2053
BstSCI CCNGG 5 cut(s) 174, 1996, 2256, 2820, 2898
BstSFI CTRYAG 7 cut(s) 252, 530, 682, 1055, 1449, 2160, 2403
BstSNI TACGTA 1 cut(s) 347
BstUI CGCG 2 cut(s) 90, 144
BstV1I GCAGC 4 cut(s) 46, 224, 2017, 2736
BstV2I GAAGAC 1 cut(s) 1179
BstX2I RGATCY 1 cut(s) 1445
BstXI CCANNNNNNTGG 2 cut(s) 367, 2982
BstYI RGATCY 1 cut(s) 1445
BstZ17I GTATAC 1 cut(s) 446
BstZI CGGCCG 1 cut(s) 85
Bsu15I ATCGAT 2 cut(s) 1914, 3017
Bsu36I CCTNAGG 1 cut(s) 3081
BsuI GTATCC 1 cut(s) 848
BsuTUI ATCGAT 2 cut(s) 1914, 3017
BtgI CCRYGG 4 cut(s) 376, 567, 2737, 2872
BtgZI GCGATG 1 cut(s) 1210
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 7 cut(s) 757, 1512, 1567, 1723, 1938, 2122, 2392
BtsIMutI CAGTG 6 cut(s) 1143, 1280, 1505, 2439, 2925, 2967
BveI ACCTGC 2 cut(s) 21, 1513
Cac8I GCNNGC 8 cut(s) 144, 249, 1348, 2344, 2965, 2984, 3026, 3118
CaiI CAGNNNCTG 2 cut(s) 69, 1256
CfoI GCGC 4 cut(s) 144, 146, 1653, 2957
Cfr10I RCCGGY 1 cut(s) 2057
Cfr13I GGNCC 8 cut(s) 78, 996, 1984, 2135, 2205, 2286, 2634, 3107
ClaI ATCGAT 2 cut(s) 1914, 3017
CseI GACGC 3 cut(s) 79, 120, 1982
Csp6I GTAC 7 cut(s) 594, 1473, 2045, 2400, 2437, 2652, 2972
CspAI ACCGGT 1 cut(s) 2057
CspCI CAANNNNNGTGG 2 cut(s) 564, 599
CviQI GTAC 7 cut(s) 594, 1473, 2045, 2400, 2437, 2652, 2972
DpnI GATC 7 cut(s) 132, 267, 502, 1175, 1447, 2498, 3016
DpnII GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
DraI TTTAAA 2 cut(s) 399, 474
DrdI GACNNNNNNGTC 1 cut(s) 117
DseDI GACNNNNNNGTC 1 cut(s) 117
EaeI YGGCCR 3 cut(s) 85, 605, 2415
EagI CGGCCG 1 cut(s) 85
Eam1104I CTCTTC 2 cut(s) 285, 1827
EarI CTCTTC 2 cut(s) 285, 1827
EciI GGCGGA 1 cut(s) 68
EclXI CGGCCG 1 cut(s) 85
Eco105I TACGTA 1 cut(s) 347
Eco130I CCWWGG 3 cut(s) 360, 376, 2229
Eco31I GGTCTC 2 cut(s) 813, 1040
Eco32I GATATC 1 cut(s) 1912
Eco47I GGWCC 6 cut(s) 996, 1984, 2135, 2205, 2286, 3107
Eco52I CGGCCG 1 cut(s) 85
Eco57I CTGAAG 3 cut(s) 309, 618, 1278
Eco81I CCTNAGG 1 cut(s) 3081
Eco88I CYCGRG 1 cut(s) 1827
Eco91I GGTNACC 1 cut(s) 2053
EcoNI CCTNNNNNAGG 2 cut(s) 17, 1520
EcoO109I RGGNCCY 2 cut(s) 1984, 2634
EcoO65I GGTNACC 1 cut(s) 2053
EcoRII CCWGG 4 cut(s) 174, 1996, 2820, 2898
EcoRV GATATC 1 cut(s) 1912
EcoT14I CCWWGG 3 cut(s) 360, 376, 2229
EcoT22I ATGCAT 3 cut(s) 222, 2809, 3053
ErhI CCWWGG 3 cut(s) 360, 376, 2229
FalI AAGNNNNNCTT 2 cut(s) 2213, 2245
FaqI GGGAC 4 cut(s) 2148, 2299, 2439, 2755
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 1413
FbaI TGATCA 2 cut(s) 265, 2496
FblI GTMKAC 1 cut(s) 445
Fnu4HI GCNGC 5 cut(s) 35, 88, 213, 2031, 2750
FokI GGATG 7 cut(s) 764, 1499, 1574, 1710, 1925, 2129, 2399
Fsp4HI GCNGC 5 cut(s) 35, 88, 213, 2031, 2750
FspBI CTAG 5 cut(s) 747, 1368, 2091, 2753, 2964
GlaI GCGC 4 cut(s) 143, 145, 1652, 2956
GluI GCNGC 5 cut(s) 35, 88, 213, 2031, 2750
GsaI CCCAGC 3 cut(s) 1651, 2037, 2893
GsuI CTGGAG 4 cut(s) 91, 255, 1521, 3096
HapII CCGG 3 cut(s) 830, 2058, 2257
HgaI GACGC 3 cut(s) 79, 120, 1982
HhaI GCGC 4 cut(s) 144, 146, 1653, 2957
Hin6I GCGC 4 cut(s) 142, 144, 1651, 2955
HinP1I GCGC 4 cut(s) 142, 144, 1651, 2955
HincII GTYRAC 3 cut(s) 2472, 2479, 2563
HindII GTYRAC 3 cut(s) 2472, 2479, 2563
HindIII AAGCTT 2 cut(s) 880, 2656
HinfI GANTC 7 cut(s) 633, 875, 1285, 1598, 1919, 1977, 2188
HpaI GTTAAC 1 cut(s) 2479
HpaII CCGG 3 cut(s) 830, 2058, 2257
Hpy166II GTNNAC 6 cut(s) 446, 1622, 2437, 2472, 2479, 2563
Hpy188I TCNGA 7 cut(s) 289, 1492, 1549, 1765, 2493, 2697, 2770
Hpy8I GTNNAC 6 cut(s) 446, 1622, 2437, 2472, 2479, 2563
Hpy99I CGWCG 3 cut(s) 32, 86, 95
HpyAV CCTTC 7 cut(s) 299, 419, 593, 605, 1253, 1999, 2935
HpyCH4IV ACGT 4 cut(s) 30, 346, 2428, 2481
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 134, 143, 694, 1877
HpySE526I ACGT 4 cut(s) 30, 346, 2428, 2481
HspAI GCGC 4 cut(s) 142, 144, 1651, 2955
Ksp22I TGATCA 2 cut(s) 265, 2496
KspAI GTTAAC 1 cut(s) 2479
Kzo9I GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
LmnI GCTCC 3 cut(s) 1502, 2302, 2536
Lsp1109I GCAGC 4 cut(s) 46, 224, 2017, 2736
LweI GCATC 8 cut(s) 344, 1186, 1345, 1633, 1769, 2284, 2518, 2761
MaeI CTAG 5 cut(s) 747, 1368, 2091, 2753, 2964
MaeII ACGT 4 cut(s) 30, 346, 2428, 2481
MalI GATC 7 cut(s) 132, 267, 502, 1175, 1447, 2498, 3016
MboI GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 1 cut(s) 1445
MhlI GDGCHC 2 cut(s) 1507, 1716
MlsI TGGCCA 2 cut(s) 607, 2417
MluNI TGGCCA 2 cut(s) 607, 2417
MlyI GAGTC 3 cut(s) 884, 1294, 1607
MmeI TCCRAC 2 cut(s) 772, 1954
Mox20I TGGCCA 2 cut(s) 607, 2417
Mph1103I ATGCAT 3 cut(s) 222, 2809, 3053
MroXI GAANNNNTTC 2 cut(s) 2252, 3096
MscI TGGCCA 2 cut(s) 607, 2417
MslI CAYNNNNRTG 4 cut(s) 324, 1728, 2231, 2570
Msp20I TGGCCA 2 cut(s) 607, 2417
MspA1I CMGCKG 3 cut(s) 2749, 2889, 3116
MspI CCGG 3 cut(s) 830, 2058, 2257
MspR9I CCNGG 5 cut(s) 176, 1998, 2258, 2822, 2900
MunI CAATTG 1 cut(s) 576
Mva1269I GAATGC 3 cut(s) 315, 2176, 2807
MvaI CCWGG 4 cut(s) 176, 1998, 2822, 2900
MvnI CGCG 2 cut(s) 90, 144
MwoI GCNNNNNNNGC 5 cut(s) 40, 134, 143, 694, 1877
NciI CCSGG 1 cut(s) 2258
NcoI CCATGG 1 cut(s) 376
NdeI CATATG 1 cut(s) 1413
NdeII GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
NheI GCTAGC 1 cut(s) 2963
NlaIV GGNNCC 6 cut(s) 943, 1680, 1986, 2136, 2287, 2636
NmeAIII GCCGAG 3 cut(s) 29, 172, 178
NmuCI GTSAC 6 cut(s) 412, 677, 742, 1117, 2053, 2060
NsiI ATGCAT 3 cut(s) 222, 2809, 3053
NspI RCATGY 4 cut(s) 619, 2517, 2881, 3028
OliI CACNNNNGTG 1 cut(s) 2231
PaeI GCATGC 1 cut(s) 3028
PauI GCGCGC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 3 cut(s) 315, 2176, 2807
PdmI GAANNNNTTC 2 cut(s) 2252, 3096
PfeI GAWTC 4 cut(s) 633, 1919, 1977, 2188
PflMI CCANNNNNTGG 2 cut(s) 69, 1431
PfoI TCCNGGA 2 cut(s) 174, 2256
PinAI ACCGGT 1 cut(s) 2057
PkrI GCNGC 5 cut(s) 36, 89, 214, 2032, 2751
PleI GAGTC 3 cut(s) 883, 1293, 1606
PpsI GAGTC 3 cut(s) 883, 1293, 1606
Ppu21I YACGTR 1 cut(s) 347
PpuMI RGGWCCY 1 cut(s) 1984
PshBI ATTAAT 2 cut(s) 1310, 1961
PsiI TTATAA 1 cut(s) 3008
Psp1406I AACGTT 1 cut(s) 2481
Psp5II RGGWCCY 1 cut(s) 1984
Psp6I CCWGG 4 cut(s) 174, 1996, 2820, 2898
PspEI GGTNACC 1 cut(s) 2053
PspFI CCCAGC 3 cut(s) 1647, 2033, 2889
PspGI CCWGG 4 cut(s) 174, 1996, 2820, 2898
PspN4I GGNNCC 6 cut(s) 943, 1680, 1986, 2136, 2287, 2636
PspPI GGNCC 8 cut(s) 78, 996, 1984, 2135, 2205, 2286, 2634, 3107
PspPPI RGGWCCY 1 cut(s) 1984
PstI CTGCAG 1 cut(s) 2164
PstNI CAGNNNCTG 2 cut(s) 69, 1256
PsuI RGATCY 1 cut(s) 1445
PteI GCGCGC 1 cut(s) 142
PvuII CAGCTG 3 cut(s) 2749, 2889, 3116
RsaI GTAC 7 cut(s) 595, 1474, 2046, 2401, 2438, 2653, 2973
RsaNI GTAC 7 cut(s) 594, 1473, 2045, 2400, 2437, 2652, 2972
RseI CAYNNNNRTG 4 cut(s) 324, 1728, 2231, 2570
SatI GCNGC 5 cut(s) 35, 88, 213, 2031, 2750
Sau3AI GATC 7 cut(s) 130, 265, 500, 1173, 1445, 2496, 3014
Sau96I GGNCC 8 cut(s) 78, 996, 1984, 2135, 2205, 2286, 2634, 3107
ScaI AGTACT 1 cut(s) 2401
SchI GAGTC 3 cut(s) 884, 1294, 1607
ScrFI CCNGG 5 cut(s) 176, 1998, 2258, 2822, 2900
SduI GDGCHC 2 cut(s) 1507, 1716
SfaNI GCATC 8 cut(s) 344, 1186, 1345, 1633, 1769, 2284, 2518, 2761
SfcI CTRYAG 7 cut(s) 252, 530, 682, 1055, 1449, 2160, 2403
SgrAI CRCCGGYG 1 cut(s) 2057
SinI GGWCC 6 cut(s) 996, 1984, 2135, 2205, 2286, 3107
SmiMI CAYNNNNRTG 4 cut(s) 324, 1728, 2231, 2570
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
SnaBI TACGTA 1 cut(s) 347
SphI GCATGC 1 cut(s) 3028
SsiI CCGC 4 cut(s) 53, 88, 105, 1461
SspMI CTAG 5 cut(s) 747, 1368, 2091, 2753, 2964
StyD4I CCNGG 5 cut(s) 174, 1996, 2256, 2820, 2898
StyI CCWWGG 3 cut(s) 360, 376, 2229
TaiI ACGT 4 cut(s) 33, 349, 2431, 2484
TaqI TCGA 4 cut(s) 27, 1914, 2730, 3017
TaqII GACCGA 2 cut(s) 1013, 2193
TatI WGTACW 3 cut(s) 1472, 2399, 2436
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 4 cut(s) 633, 1919, 1977, 2188
TscAI CASTG 6 cut(s) 1143, 1287, 1512, 2439, 2925, 2974
TseFI GTSAC 6 cut(s) 412, 677, 742, 1117, 2053, 2060
TseI GCWGC 4 cut(s) 34, 212, 2030, 2749
Tsp45I GTSAC 6 cut(s) 412, 677, 742, 1117, 2053, 2060
TspGWI ACGGA 6 cut(s) 107, 1193, 1397, 2634, 2726, 2861
TspRI CASTG 6 cut(s) 1143, 1287, 1512, 2439, 2925, 2974
Van91I CCANNNNNTGG 2 cut(s) 69, 1431
VpaK11BI GGWCC 6 cut(s) 996, 1984, 2135, 2205, 2286, 3107
VspI ATTAAT 2 cut(s) 1310, 1961
XagI CCTNNNNNAGG 2 cut(s) 17, 1520
XapI RAATTY 5 cut(s) 1494, 1613, 1861, 2141, 2239
XceI RCATGY 4 cut(s) 619, 2517, 2881, 3028
XcmI CCANNNNNNNNNTGG 1 cut(s) 1411
XmiI GTMKAC 1 cut(s) 445
XmnI GAANNNNTTC 2 cut(s) 2252, 3096
XspI CTAG 5 cut(s) 747, 1368, 2091, 2753, 2964
ZrmI AGTACT 1 cut(s) 2401
Zsp2I ATGCAT 3 cut(s) 222, 2809, 3053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.