Prupe.7G054200_v2.0.a1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0002172
Physical Location & Seq
Reverse (-)
9258060 .. 9265159
7100 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G054200.2

Sequence Viewer

Length: 3123 bp
ATGGAGAGGTACTTGAAGGACTTCGAGGTGGAGAGCAAGAATCCTTCGGAGGAAACTATTCGGAGATGGAGAAAAGCCGTCGCGCTCGTCAAGAACCGTCGCAGGAGGTTCCGCTTCGTGGCCGATCTCGCTAAGCGGTCCGAGGCCGAGAGGAAGAAGCGTCAAATCCAGGAAAAGATTCGAGTTGCTCTTTACGTTCAGAAAGCAGCACTGCAGTTTATTGATGCTGGTGCTGGTGATCGATCCAATGAAAAGCCAAGACAGGATGAATACAAGCTGTCTGAAGATGCCAGAACCTCAGGTTTCAGCATTCACCCAGATGAACTTGCATCTATCACGCGTGGCCATGATATTAAGGCCTTGAAAATGCATGGTGGAATTCATGGGATTTTAAGGAAAGTCTCTGTCTCGCTAGATGAAGGTGTCAAGGATAGTAACATACCAATCAGGCAAAATGTTTATGGCTTAAACCGCTATACGGAGAAACCTCCCCGAACATTTTTTGTGTTTGTATGGGAAGCCCTACAAGACTTAACATTAATTATCCTTATGGTATGCGCTGTGGTTTCAATAGGAGTTGGAATTGCCACTGAGGGGTGGCCAAAAGGCATGTACGATGGTGTGGGAATTTTAATTAGTATAGTTTTGGTGGTTATGGTTACTGCCATTAGTGACTACAGACAGTCGTTGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTGTTCAGGTCACTAGGGATAAAAAAAGACAAAAAGTTTCCATTTATGACTTGGTTGTTGGAGATATTGTCCATTTGTCAATTGGGGACCAAGTTCCAGCTGATGGGATTTTCATATCAGGATACTCTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCGGTCAATGTATATGAAGAGAAGCCTTTTCTTCTTTCAGGAACTAAAGTGCAGGATGGGTCAGGTATAATGCTGGTGACAACAGTTGGTATGAGAACTGAATGGGGAAAGTTGATGGAAACTCTGAGTGAAGGAGGAGAAGATGAGACCCCACTGCAGGTAAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTCTGAGTTTTGCTGTGTTGACATTTTTGGTATTGGCAGTAAGATTTTTGGTGGAAAAAATACTTAACAACGAGATCACTGATTGGTCTTCAACTGATGCAGTAATCCTATTGAACTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACCATTGGCAGTGACGCTGAGCCTTGCTTTTGCTATGAAAAAATTAATGAATGATAGGGCACTCGTAAGGCATCTCTCAGCATGTGAGACAATGGGTTCTGCTAGTTGCATTTGCACAGATAAAACAGGGACATTAACCACAAACCACATGGTAGTTAACAAGATATGGATATGTGAAAAACCTTTAGATGTAAAAGGTAACGAGAGTAAAGAAATATTGAGTTCAGAAATATCTGGAGCATCAAGCATCCTTTTACAGGTTATATTCCAAAATACAAGCTCTGAGGTTATTAAGGAAGATGGAAAGACCTCCATTTTGGGGACACCAACAGAGTCAGCATTATTAGAATTTGGTTTACTTTTGGGTGGTGATTTTGATGCCGTGCGCAGAGAGGTTAATATTCTTAAGGTTGAACCTTTCAACTCTGTGAGGAAGAAAATGTCTGTGCTTGTAGCTCATCCTCATGGTGGGAAACGAGCCTTTTGCAAAGGTGCATCAGAAATAGTACTGGGAATGTGTAACAAATTTATTGACTTTAATGGAGAATCTGTTATTCTCTCCCGAGAACAGGTAAAGAATATCACGGATGTCATTAATTCTTTTGCTTCTGAAGCCTTGAGAACTCTCTGCTTGGCTTTCAAAAATATAGATGACTCTTCCATAGAAAATGACATCCCAGATGATGGCTATACATTGATAGCAGTTGTCGGTATCAAGGATCCTGTGCGCCCTGGGGTCAAGGATGCAGTTCAAACTTGTTTAGCTGCTGGAATAACTGTACGAATGGTCACTGGTGACAATATAAATACCGCTAAAGCCATAGCTAAAGAATGCGGCATACTCACAGAAGATGGTTTAGCCATAGAAGGACAGGAGTTTCGTAACATGTCTCTTGAGCAGAAGAAAGCTGTTATACCGAGGATTCAGGTGATGGCTCGGTCTTTACCGTTGGATAAGCACATCTTGGTAAAAACTCTGAGAGATGAATTTGGTGAGGTAGTTGCAGTGACTGGCGATGGGACCAACGACGCTCCTGCTTTGCACGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGAACAGAGGTTGCCAAGGAAAGTGCTGATGTCATCATATTGGATGACAATTTTAAGACTATAGTAAATGTGGCCAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTGCAGTTCCAGTTGACAGTTAACGTTGTTGCTCTAATAATCAATTTTGTTTCTGCATGTGTCTCAGGATCTGCTCCCCTTACGGCTGTGCAATTGCTTTGGGTCAACATGATTATGGACACTCTTGGGGCATTGGCACTGGCAACAGAGCCTCCAAATGATGGACTTATGAAAAGGCCCCCTGTTGGGAGGGGTACAAGCTTCATAACCAAGGCCATGTGGAGGAATATAATTGGCCAGAGTATCTATCAACTGATTGTCCTTGGAGTTCTCAATTTCTATGGGAAACATCTACTTGGACTTTCTGGCTCAGATGCAACTGAAGTTCTAGACACTGTGATATTCAACGCATTTGTGTTTTGCCAGGTGTTTAATGAGATAAACAGCCGTGACATAGAGAAGATAAACATATTCGTGGGCATGTTTGATAGCTGGGTATTTCTAGGCGTCATGGTTTGCACAGTGGCCTTCCAAGTTATTATAGTAGAGTTCTTGGGAGATTTTGCTAGCACTGTTCCGCTAAGCTGGCAACTATGGTTACTCTGCATTTTACTTGGATCAGTTAGCATGCTTGTTGCAGTTGTGCTCAAGTTCATACCGGTCGAAAGTACAATTAAGCACCATGATGGATATGAACCGCTTCCTTCTGGTCCTCCCGACAGCATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1041

Amino Acids

114.25

Weight (kDa)

5.87

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1646
Acc36I ACCTGC 1 cut(s) 1042
AccB7I CCANNNNNTGG 3 cut(s) 830, 1943, 2612
AccII CGCG 2 cut(s) 83, 340
AciI CCGC 8 cut(s) 112, 136, 472, 1223, 2070, 2094, 2969, 3089
AclI AACGTT 1 cut(s) 2475
AclWI GGATC 5 cut(s) 237, 1973, 1986, 2527, 3016
AcoI YGGCCR 5 cut(s) 120, 343, 599, 2409, 2686
AcsI RAATTY 5 cut(s) 378, 627, 1607, 1784, 2246
AcuI CTGAAG 3 cut(s) 303, 1890, 2793
AcyI GRCGYC 1 cut(s) 2898
AfaI GTAC 7 cut(s) 11, 614, 1767, 2040, 2432, 2647, 3061
AflII CTTAAG 1 cut(s) 1664
AflIII ACRYGT 2 cut(s) 338, 2145
AgeI ACCGGT 1 cut(s) 3049
AjnI CCWGG 3 cut(s) 168, 1990, 2814
Alw21I GWGCWC 1 cut(s) 3039
Alw26I GTCTC 6 cut(s) 406, 412, 1034, 1340, 2154, 2518
AlwI GGATC 5 cut(s) 237, 1973, 1986, 2527, 3016
AlwNI CAGNNNCTG 2 cut(s) 2270, 2522
Ama87I CYCGRG 1 cut(s) 1821
ApeKI GCWGC 2 cut(s) 206, 2024
ApoI RAATTY 5 cut(s) 378, 627, 1607, 1784, 2246
ArsI GACNNNNNNTTYG 4 cut(s) 1558, 1590, 2121, 2153
AseI ATTAAT 3 cut(s) 539, 1304, 1854
AsiGI ACCGGT 1 cut(s) 3049
Asp700I GAANNNNTTC 4 cut(s) 20, 57, 177, 3090
AspLEI GCGC 4 cut(s) 85, 560, 1647, 1989
AspS9I GGNCC 5 cut(s) 138, 814, 2280, 2628, 3101
AsuHPI GGTGA 8 cut(s) 248, 305, 896, 982, 1640, 2066, 2200, 2264
AsuNHI GCTAGC 1 cut(s) 2957
AvaI CYCGRG 1 cut(s) 1821
AvaII GGWCC 4 cut(s) 138, 814, 2280, 3101
AxyI CCTNAGG 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 1321
BalI TGGCCA 4 cut(s) 345, 601, 2411, 2688
BamHI GGATCC 1 cut(s) 1978
BauI CACGAG 1 cut(s) 2303
BbsI GAAGAC 1 cut(s) 1173
Bbv12I GWGCWC 1 cut(s) 3039
BbvI GCAGC 2 cut(s) 218, 2011
BceAI ACGGC 4 cut(s) 62, 1625, 2550, 2823
BcgI CGANNNNNNTGC 4 cut(s) 1725, 1759, 2276, 2310
BciT130I CCWGG 3 cut(s) 170, 1992, 2816
BciVI GTATCC 1 cut(s) 842
BcoDI GTCTC 6 cut(s) 406, 412, 1034, 1340, 2154, 2518
BfaI CTAG 6 cut(s) 413, 741, 1362, 2780, 2894, 2958
BfmI CTRYAG 4 cut(s) 212, 676, 1049, 2397
BfrI CTTAAG 1 cut(s) 1664
BfuAI ACCTGC 1 cut(s) 1042
BfuI GTATCC 1 cut(s) 842
BisI GCNGC 3 cut(s) 207, 2025, 2095
BlpI GCTNAGC 3 cut(s) 132, 1277, 2972
BlsI GCNGC 3 cut(s) 208, 2026, 2096
BmcAI AGTACT 1 cut(s) 1767
Bme1390I CCNGG 3 cut(s) 170, 1992, 2816
Bme18I GGWCC 4 cut(s) 138, 814, 2280, 3101
BmeT110I CYCGRG 1 cut(s) 1821
BmgT120I GGNCC 5 cut(s) 138, 814, 2280, 2628, 3101
BmiI GGNNCC 5 cut(s) 110, 815, 1980, 2281, 2630
BmrFI CCNGG 3 cut(s) 170, 1992, 2816
BmrI ACTGGG 1 cut(s) 1778
BmtI GCTAGC 1 cut(s) 2961
BmuI ACTGGG 1 cut(s) 1778
BpiI GAAGAC 1 cut(s) 1173
BpmI CTGGAG 1 cut(s) 1515
Bpu1102I GCTNAGC 3 cut(s) 132, 1277, 2972
BpuEI CTTGAG 3 cut(s) 1897, 2174, 3023
Bsa29I ATCGAT 1 cut(s) 241
BsaHI GRCGYC 1 cut(s) 2898
BsaI GGTCTC 1 cut(s) 1034
BsaJI CCNNGG 7 cut(s) 141, 1990, 1991, 2177, 2352, 2661, 2713
BsaWI WCCGGW 1 cut(s) 3049
BsaXI ACNNNNNCTCC 6 cut(s) 780, 810, 1794, 1824, 2587, 2617
Bse118I RCCGGY 2 cut(s) 895, 3049
Bse1I ACTGG 5 cut(s) 1773, 2056, 2275, 2461, 2595
Bse21I CCTNAGG 1 cut(s) 298
BseBI CCWGG 3 cut(s) 170, 1992, 2816
BseCI ATCGAT 1 cut(s) 241
BseDI CCNNGG 7 cut(s) 141, 1990, 1991, 2177, 2352, 2661, 2713
BseGI GGATG 8 cut(s) 271, 955, 1506, 1717, 1852, 1932, 2008, 2386
BseNI ACTGG 5 cut(s) 1773, 2056, 2275, 2461, 2595
BseRI GAGGAG 1 cut(s) 1044
BseSI GKGCMC 1 cut(s) 1321
BseXI GCAGC 2 cut(s) 218, 2011
BseYI CCCAGC 1 cut(s) 2883
BsgI GTGCAG 2 cut(s) 965, 2474
Bsh1236I CGCG 2 cut(s) 83, 340
Bsh1285I CGRYCG 1 cut(s) 3054
BshTI ACCGGT 1 cut(s) 3049
BshVI ATCGAT 1 cut(s) 241
BsiEI CGRYCG 1 cut(s) 3054
BsiHKAI GWGCWC 1 cut(s) 3039
BsiHKCI CYCGRG 1 cut(s) 1821
BsiSI CCGG 2 cut(s) 896, 3050
BslFI GGGAC 5 cut(s) 827, 1402, 1594, 2293, 2433
BsmAI GTCTC 6 cut(s) 406, 412, 1034, 1340, 2154, 2518
BsmFI GGGAC 5 cut(s) 827, 1402, 1594, 2293, 2433
BsmI GAATGC 2 cut(s) 309, 2096
Bso31I GGTCTC 1 cut(s) 1034
BsoBI CYCGRG 1 cut(s) 1821
Bsp1286I GDGCHC 2 cut(s) 1321, 3039
Bsp1407I TGTACA 1 cut(s) 2430
Bsp143I GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
Bsp1720I GCTNAGC 3 cut(s) 132, 1277, 2972
BspACI CCGC 8 cut(s) 112, 136, 472, 1223, 2070, 2094, 2969, 3089
BspDI ATCGAT 1 cut(s) 241
BspFNI CGCG 2 cut(s) 83, 340
BspLI GGNNCC 5 cut(s) 110, 815, 1980, 2281, 2630
BspMAI CTGCAG 2 cut(s) 216, 1053
BspMI ACCTGC 1 cut(s) 1042
BspOI GCTAGC 1 cut(s) 2961
BspPI GGATC 5 cut(s) 237, 1973, 1986, 2527, 3016
BspTI CTTAAG 1 cut(s) 1664
BspTNI GGTCTC 1 cut(s) 1034
BsrFI RCCGGY 2 cut(s) 895, 3049
BsrGI TGTACA 1 cut(s) 2430
BsrI ACTGG 5 cut(s) 1773, 2056, 2275, 2461, 2595
BssAI RCCGGY 2 cut(s) 895, 3049
BssECI CCNNGG 7 cut(s) 141, 1990, 1991, 2177, 2352, 2661, 2713
BssMI GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
BssNI GRCGYC 1 cut(s) 2898
BssSI CACGAG 1 cut(s) 2303
BssT1I CCWWGG 3 cut(s) 2352, 2661, 2713
Bst2BI CACGAG 1 cut(s) 2303
Bst2UI CCWGG 3 cut(s) 170, 1992, 2816
Bst4CI ACNGT 9 cut(s) 98, 684, 979, 2038, 2208, 2470, 2788, 2914, 2965
Bst6I CTCTTC 2 cut(s) 906, 1921
BstACI GRCGYC 1 cut(s) 2898
BstAFI CTTAAG 1 cut(s) 1664
BstAUI TGTACA 1 cut(s) 2430
BstC8I GCNNGC 3 cut(s) 2959, 2978, 3020
BstENI CCTNNNNNAGG 1 cut(s) 1514
BstF5I GGATG 8 cut(s) 271, 955, 1506, 1717, 1852, 1932, 2008, 2386
BstFNI CGCG 2 cut(s) 83, 340
BstHHI GCGC 4 cut(s) 85, 560, 1647, 1989
BstKTI GATC 7 cut(s) 127, 241, 245, 1170, 1981, 2522, 3011
BstMAI GTCTC 6 cut(s) 406, 412, 1034, 1340, 2154, 2518
BstMBI GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
BstMCI CGRYCG 1 cut(s) 3054
BstMWI GCNNNNNNNGC 5 cut(s) 128, 471, 1057, 1871, 2977
BstNI CCWGG 3 cut(s) 170, 1992, 2816
BstNSI RCATGY 6 cut(s) 613, 1344, 2149, 2511, 2875, 3022
BstSCI CCNGG 3 cut(s) 168, 1990, 2814
BstSFI CTRYAG 4 cut(s) 212, 676, 1049, 2397
BstSLI GKGCMC 1 cut(s) 1321
BstUI CGCG 2 cut(s) 83, 340
BstV1I GCAGC 2 cut(s) 218, 2011
BstV2I GAAGAC 1 cut(s) 1173
BstX2I RGATCY 2 cut(s) 1978, 2519
BstYI RGATCY 2 cut(s) 1978, 2519
Bsu15I ATCGAT 1 cut(s) 241
Bsu36I CCTNAGG 1 cut(s) 298
BsuI GTATCC 1 cut(s) 842
BsuTUI ATCGAT 1 cut(s) 241
BtgZI GCGATG 1 cut(s) 2289
BtsCI GGATG 8 cut(s) 271, 955, 1506, 1717, 1852, 1932, 2008, 2386
BtsI GCAGTG 4 cut(s) 209, 1046, 1275, 2271
BveI ACCTGC 1 cut(s) 1042
Cac8I GCNNGC 3 cut(s) 2959, 2978, 3020
CaiI CAGNNNCTG 2 cut(s) 2270, 2522
CfoI GCGC 4 cut(s) 85, 560, 1647, 1989
Cfr10I RCCGGY 2 cut(s) 895, 3049
Cfr13I GGNCC 5 cut(s) 138, 814, 2280, 2628, 3101
ClaI ATCGAT 1 cut(s) 241
CpoI CGGWCCG 1 cut(s) 138
CseI GACGC 4 cut(s) 149, 1282, 2297, 2887
Csp6I GTAC 7 cut(s) 10, 613, 1766, 2039, 2431, 2646, 3060
CspAI ACCGGT 1 cut(s) 3049
CspI CGGWCCG 1 cut(s) 138
CviQI GTAC 7 cut(s) 10, 613, 1766, 2039, 2431, 2646, 3060
DpnI GATC 7 cut(s) 126, 240, 244, 1169, 1980, 2521, 3010
DpnII GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
EaeI YGGCCR 5 cut(s) 120, 343, 599, 2409, 2686
Eam1104I CTCTTC 2 cut(s) 906, 1921
EarI CTCTTC 2 cut(s) 906, 1921
Eco130I CCWWGG 3 cut(s) 2352, 2661, 2713
Eco147I AGGCCT 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 1034
Eco47I GGWCC 4 cut(s) 138, 814, 2280, 3101
Eco57I CTGAAG 3 cut(s) 303, 1890, 2793
Eco81I CCTNAGG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 1821
EcoNI CCTNNNNNAGG 1 cut(s) 1514
EcoO109I RGGNCCY 1 cut(s) 2628
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 3 cut(s) 168, 1990, 2814
EcoT14I CCWWGG 3 cut(s) 2352, 2661, 2713
EcoT22I ATGCAT 1 cut(s) 372
ErhI CCWWGG 3 cut(s) 2352, 2661, 2713
FalI AAGNNNNNCTT 2 cut(s) 2207, 2239
FaqI GGGAC 5 cut(s) 827, 1402, 1594, 2293, 2433
Fnu4HI GCNGC 3 cut(s) 207, 2025, 2095
FokI GGATG 8 cut(s) 278, 962, 1493, 1704, 1859, 1919, 2015, 2393
Fsp4HI GCNGC 3 cut(s) 207, 2025, 2095
FspBI CTAG 6 cut(s) 413, 741, 1362, 2780, 2894, 2958
FspI TGCGCA 1 cut(s) 1646
GlaI GCGC 4 cut(s) 84, 559, 1646, 1988
GluI GCNGC 3 cut(s) 207, 2025, 2095
GsaI CCCAGC 1 cut(s) 2887
GsuI CTGGAG 1 cut(s) 1515
HapII CCGG 2 cut(s) 896, 3050
HgaI GACGC 4 cut(s) 149, 1282, 2297, 2887
HhaI GCGC 4 cut(s) 85, 560, 1647, 1989
Hin1I GRCGYC 1 cut(s) 2898
Hin6I GCGC 4 cut(s) 83, 558, 1645, 1987
HinP1I GCGC 4 cut(s) 83, 558, 1645, 1987
HincII GTYRAC 5 cut(s) 1113, 1417, 2466, 2473, 2557
HindII GTYRAC 5 cut(s) 1113, 1417, 2466, 2473, 2557
HindIII AAGCTT 2 cut(s) 874, 2650
HinfI GANTC 7 cut(s) 40, 178, 869, 1592, 1805, 1913, 2182
HpaI GTTAAC 2 cut(s) 1417, 2473
HpaII CCGG 2 cut(s) 896, 3050
HphI GGTGA 8 cut(s) 248, 305, 896, 982, 1640, 2066, 2200, 2264
Hpy166II GTNNAC 7 cut(s) 1113, 1417, 1616, 2431, 2466, 2473, 2557
Hpy8I GTNNAC 7 cut(s) 1113, 1417, 1616, 2431, 2466, 2473, 2557
Hpy99I CGWCG 3 cut(s) 83, 102, 2291
HpyAV CCTTC 8 cut(s) 10, 54, 413, 1019, 1247, 2120, 2929, 3105
HpyCH4III ACNGT 9 cut(s) 98, 684, 979, 2038, 2208, 2470, 2788, 2914, 2965
HpyCH4IV ACGT 3 cut(s) 195, 2422, 2475
HpyF10VI GCNNNNNNNGC 5 cut(s) 128, 471, 1057, 1871, 2977
HpySE526I ACGT 3 cut(s) 195, 2422, 2475
Hsp92I GRCGYC 1 cut(s) 2898
HspAI GCGC 4 cut(s) 83, 558, 1645, 1987
KspAI GTTAAC 2 cut(s) 1417, 2473
Kzo9I GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
LmnI GCTCC 3 cut(s) 1496, 2296, 2530
Lsp1109I GCAGC 2 cut(s) 218, 2011
MaeI CTAG 6 cut(s) 413, 741, 1362, 2780, 2894, 2958
MaeII ACGT 3 cut(s) 195, 2422, 2475
MalI GATC 7 cut(s) 126, 240, 244, 1169, 1980, 2521, 3010
MboI GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
MfeI CAATTG 2 cut(s) 807, 2543
MflI RGATCY 2 cut(s) 1978, 2519
MhlI GDGCHC 2 cut(s) 1321, 3039
MlsI TGGCCA 4 cut(s) 345, 601, 2411, 2688
MluI ACGCGT 1 cut(s) 338
MluNI TGGCCA 4 cut(s) 345, 601, 2411, 2688
MlyI GAGTC 3 cut(s) 878, 1601, 1907
MmeI TCCRAC 3 cut(s) 559, 766, 2190
Mox20I TGGCCA 4 cut(s) 345, 601, 2411, 2688
Mph1103I ATGCAT 1 cut(s) 372
MroXI GAANNNNTTC 4 cut(s) 20, 57, 177, 3090
MscI TGGCCA 4 cut(s) 345, 601, 2411, 2688
MslI CAYNNNNRTG 5 cut(s) 318, 1722, 2564, 2864, 3075
Msp20I TGGCCA 4 cut(s) 345, 601, 2411, 2688
MspA1I CMGCKG 1 cut(s) 827
MspCI CTTAAG 1 cut(s) 1664
MspI CCGG 2 cut(s) 896, 3050
MspR9I CCNGG 3 cut(s) 170, 1992, 2816
MunI CAATTG 2 cut(s) 807, 2543
Mva1269I GAATGC 2 cut(s) 309, 2096
MvaI CCWGG 3 cut(s) 170, 1992, 2816
MvnI CGCG 2 cut(s) 83, 340
MwoI GCNNNNNNNGC 5 cut(s) 128, 471, 1057, 1871, 2977
NdeII GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
NheI GCTAGC 1 cut(s) 2957
NlaIV GGNNCC 5 cut(s) 110, 815, 1980, 2281, 2630
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 8 cut(s) 671, 736, 970, 1270, 2047, 2054, 2266, 2840
NsbI TGCGCA 1 cut(s) 1646
NsiI ATGCAT 1 cut(s) 372
NspI RCATGY 6 cut(s) 613, 1344, 2149, 2511, 2875, 3022
PaeI GCATGC 1 cut(s) 3022
PasI CCCWGGG 1 cut(s) 1991
PceI AGGCCT 1 cut(s) 359
PciI ACATGT 1 cut(s) 2145
PctI GAATGC 2 cut(s) 309, 2096
PdmI GAANNNNTTC 4 cut(s) 20, 57, 177, 3090
PfeI GAWTC 4 cut(s) 40, 178, 1805, 2182
PflMI CCANNNNNTGG 3 cut(s) 830, 1943, 2612
PfoI TCCNGGA 1 cut(s) 168
PinAI ACCGGT 1 cut(s) 3049
PkrI GCNGC 3 cut(s) 208, 2026, 2096
PleI GAGTC 3 cut(s) 877, 1600, 1907
PpsI GAGTC 3 cut(s) 877, 1600, 1907
PscI ACATGT 1 cut(s) 2145
PshBI ATTAAT 3 cut(s) 539, 1304, 1854
Psp1406I AACGTT 1 cut(s) 2475
Psp6I CCWGG 3 cut(s) 168, 1990, 2814
PspFI CCCAGC 1 cut(s) 2883
PspGI CCWGG 3 cut(s) 168, 1990, 2814
PspN4I GGNNCC 5 cut(s) 110, 815, 1980, 2281, 2630
PspPI GGNCC 5 cut(s) 138, 814, 2280, 2628, 3101
PstI CTGCAG 2 cut(s) 216, 1053
PstNI CAGNNNCTG 2 cut(s) 2270, 2522
PsuI RGATCY 2 cut(s) 1978, 2519
PvuII CAGCTG 1 cut(s) 827
RsaI GTAC 7 cut(s) 11, 614, 1767, 2040, 2432, 2647, 3061
RsaNI GTAC 7 cut(s) 10, 613, 1766, 2039, 2431, 2646, 3060
RseI CAYNNNNRTG 5 cut(s) 318, 1722, 2564, 2864, 3075
Rsr2I CGGWCCG 1 cut(s) 138
RsrII CGGWCCG 1 cut(s) 138
SatI GCNGC 3 cut(s) 207, 2025, 2095
Sau3AI GATC 7 cut(s) 124, 238, 242, 1167, 1978, 2519, 3008
Sau96I GGNCC 5 cut(s) 138, 814, 2280, 2628, 3101
ScaI AGTACT 1 cut(s) 1767
SchI GAGTC 3 cut(s) 878, 1601, 1907
ScrFI CCNGG 3 cut(s) 170, 1992, 2816
SduI GDGCHC 2 cut(s) 1321, 3039
SfcI CTRYAG 4 cut(s) 212, 676, 1049, 2397
SinI GGWCC 4 cut(s) 138, 814, 2280, 3101
SmiMI CAYNNNNRTG 5 cut(s) 318, 1722, 2564, 2864, 3075
SmlI CTYRAG 4 cut(s) 1664, 1876, 2153, 3038
SmoI CTYRAG 4 cut(s) 1664, 1876, 2153, 3038
SphI GCATGC 1 cut(s) 3022
SseBI AGGCCT 1 cut(s) 359
SsiI CCGC 8 cut(s) 112, 136, 472, 1223, 2070, 2094, 2969, 3089
SspI AATATT 2 cut(s) 1476, 1660
SspMI CTAG 6 cut(s) 413, 741, 1362, 2780, 2894, 2958
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 3 cut(s) 168, 1990, 2814
StyI CCWWGG 3 cut(s) 2352, 2661, 2713
TaaI ACNGT 9 cut(s) 98, 684, 979, 2038, 2208, 2470, 2788, 2914, 2965
TaiI ACGT 3 cut(s) 198, 2425, 2478
TaqI TCGA 4 cut(s) 24, 181, 241, 3054
TaqII GACCGA 1 cut(s) 2187
TatI WGTACW 3 cut(s) 1765, 2430, 3059
TauI GCSGC 1 cut(s) 2097
TfiI GAWTC 4 cut(s) 40, 178, 1805, 2182
TseFI GTSAC 8 cut(s) 671, 736, 970, 1270, 2047, 2054, 2266, 2840
TseI GCWGC 2 cut(s) 206, 2024
Tsp45I GTSAC 8 cut(s) 671, 736, 970, 1270, 2047, 2054, 2266, 2840
TspGWI ACGGA 2 cut(s) 494, 1859
Van91I CCANNNNNTGG 3 cut(s) 830, 1943, 2612
Vha464I CTTAAG 1 cut(s) 1664
VpaK11BI GGWCC 4 cut(s) 138, 814, 2280, 3101
VspI ATTAAT 3 cut(s) 539, 1304, 1854
XagI CCTNNNNNAGG 1 cut(s) 1514
XapI RAATTY 5 cut(s) 378, 627, 1607, 1784, 2246
XbaI TCTAGA 1 cut(s) 2779
XceI RCATGY 6 cut(s) 613, 1344, 2149, 2511, 2875, 3022
XcmI CCANNNNNNNNNTGG 3 cut(s) 775, 806, 1405
XmnI GAANNNNTTC 4 cut(s) 20, 57, 177, 3090
XspI CTAG 6 cut(s) 413, 741, 1362, 2780, 2894, 2958
ZrmI AGTACT 1 cut(s) 1767
Zsp2I ATGCAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.