Prupe.7G054300_v2.0.a1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0002172
Physical Location & Seq
Reverse (-)
9281092 .. 9290764
9673 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G054300.8

Sequence Viewer

Length: 3984 bp
ATGGAGAGGTACTTGAAGGACTTCGAGGTGGAGAACAAGAATCCTTCGGAGGAAGCTATTCGGAGATGGAGAAAAGCCGTCGCGGTCGTCAAGAACCCTCGCAGGAGGTTCCGCTACGTCGCCGATCTCGCTAAGCGGTCCGAGGCCGAGAAGAAGAAGCTTCAAAGACAGGAAAAGATTCGAGTTGCTCTTTATGTTCAGCAAGCAGCACTGCATTTTATTGAAGCTGGTGCTGGTGATCGATCCAATGAAAAGCCAGGACAGGATGAATACAAGCTGTCTGAAGATGCCAGAACTGCAGGTTTCAGCATTCACCCAGATGAACTTGCATCTATCATGTGTGGCCATGATATTAAGGCCTCGAAAATGCATGGTGCTGGTGATCGATCCAATGAAAAGCCAGGACAGGATGAATACAAGCTGTCTGAAGATGCCAGAACTGCAGGTTTCAGCATTCACCGAGATGAACTTGCATCTATCACGTGTGGCCATGATATTAAGGCCTTGAAAATGCATGGTGGAATTCATGGGATTTTAAGGAAAGTCTGTGTCTCGCTAGATGAAGGTGTCAAGGATAGTAACATACCAATCAGGCAAAATGTTTATGGCCTAAACCGCTATACGGAGAAACCTCCCGGAACATTTTTTATGTTTGTATGGGAAGCCCTACAAGACTTAACATTAATTATCCTTATGGTATGCGCTGTGGTTTCAATAGGAGTTGAAATTGCCACTGAGGGTTGGCCAAAAGGCATGTACAATGGTGTGGGAATTTTAACTAGTATAGTTTTAGTGGTTATGGTTACTGCTATTAGTGACTACAGACAGTCGTTGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTGTTCAGGTCACTAGGGATAAAAAAAGACAAAAAGTTTCCATTTATGACTTGGTTGTTGGAGATATTGTCCATTTGTCAATTGGGGACCAAGTTCCAGCTGATGGGATTTTCATATCAGGATACTCTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCGGTCAATGTATATGAAGAGAAGCCTTTTCTTCTTTCAGGAACTAAAGTGCAGGATGGGTCAGGTATAATGCTGGTGACAACAGTTGGTATGAGAACTGAATGGGGAAAGTTGATGGAAACTCTGAGTGAAGGAGGAGAAGATGAGACCCCACTGCAGGTAAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTCTGAGTTTTGCTGTGTTGACATTTTTGGTATTGGCAGTAAGATTTTTGGTGGAAAAAATACTTAACAACGAGATCACTGATTGGTCTTCAACTGATGCAGTAATCCTATTGAACTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACCATTGGCAGTGCCGCTGAGCCTTGCTTTTGCTATGAAAAAATTAATGAATGATAGGGCACTAGTAAGGCATCTCTCAGCATGTGAGACAATGGGTTATGCTAGTTGCATTTGCACAGACAAAACAGGGACATTAACCACAACCCACATGGTAGTTAACAAGCTATGGATATGTGAAAAAACTTTAGATGTAAAAGGTAATGAGAGTAAAGAAATATTGAGTTCAGAAATATCTGGAGCATCAAGCATCCTTTTACAGGTTATATTCCAAAATACAAGCTCTGAGGTTATTAAGGAAGATGGAAGGACCTCCATTTTGGGGACACCAACAGAGTCGGCATTATTAGAATTTGGTTTACTTTTGGGTGGCGATTTTGATGCCGTGCGCAGAGAGGTTAATATTCTTAAGGTTGAACCTTTCAATTCTGTGAGGAAGAAAATGTCTGTGCTTGTAGCTCATCCTCATGGTGGTAAACGCGCCTTTTGCAAAGGTGCATCAGAAATAGTATTGGGAATGTGTAACAAATTTATTGACTTTAAAGGAGAATCTGTTATTCTCTCCCGAGAACAGGTAAAGAATATCACAGATGTCATTAATTCTTTTGCTTCTGAAGCCTTGAGAACGCTCTGCTTGGCTTTCAAAAATATAGATGACTCTTCCATCGAAAATGACATCCCAGATGATGGCTATACATTGATAGCAGTTGTCGGTATCAAGGATCCTGTGCGCCCTGGGGTAAAGGACGCAGTTCAAACTTGTTTATCTGCTGGAATAACTGTACGAATGGTCACTGGTGACAATATATATACAGCTAAAGCCATAGCTAAAGAATGCGGCATACTCACAGAAGATGGTTTAGCCATAGAAGGACAGGAGTTTCGTAACATGTCTCTTGAGCAGAAGAAAGCTGTTATACCGAGGATTCAGGTGATGGCTCGGTCTTTACCATTGGATAAGCACACCTTGGTAAAAACTCTGAGAGATGAATTTGGTGAGGTAGTTGCAGTGACTGGCGATGGGGCCGACGACGCTCCTGCTTTGCACAAGGCAGACATTAGACTTGCTATGGGCATAGCAGGAACAGAGGTTGCCAAGAAAAGTGCTGATGTCGTCATATTGGATGACAATTTTAAGACTATAGTAAATGTGGCAAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTGCAGTTCCAGTTGACAGTTAACGTTGTTGCTCTAATAATCAATTTTGTTTCTGCATGTGTCTCAGGATCTGCTTCCCTTACGGCTGTGCAATTGCTTTGGGTCAGCATGATTATGGACACTCTTGGGGCATTGGCACTGGGAACAGAGCCTCCAAGTGATGGACTTATAAAAAGGCCCTCTGTTGGTTGGGGTACAAGCTTCATAACCAAGGCCATGTGGAGGAATATCATTGGCCAGAGTATCTATCAACTGATTGTCCTTGGAGTTCTCAATTTCTATGGGAAACAGCTTCTTGGACTTTCTGGTTCAGATGCAACTGAAGTTCTAGACACACTGATATTCAACGCATTTGTGTTTTGCCAGGTGTTTAATGAGATAAACAGCCGTGACATAGAGAAGATAAACATATTCGTGGGCATGTTTGACAGCCGGGTATTCCTAGGCGTCGTGGTTTGCACAGTGGCCTTCCAAGTTATCATAGTAGAGTTCTTGGGAGCTTTTGCTAGCACTGTTCCACTAAGCTGGCAACTATGGTTACTCTGCATTTTACTTGGATCAGTTAGCATGCTTGTTGCAGTTGTGCTCAAGAACCCTTGCAGGAGGTTCCGCTTCGTCGCTGATCTCGTTAAGCGGTCCGAGGCCGACAAGAAGAAGTGTCAAATCCAGATGCCCCTTCATTCTGTCCCAGGCTCATCAACCACAGCCTCCTTTGCTGAACCAGAATTGGCAGAGTTTGAAGCTATGGACCTGGATGCTCAGCTGCAGAGGATTGAAAAACTAGGATCCACTCCAAGCAAATCCAAGTCCGAAGCAGTGGATGAGGCGGTGGACAGAATAAAAATCTGGCAGTCCACTGAGCTGGATCTGGATGAGAACAAAGAAATTATTGATAAACTGATGAAAGATCTGGACCTGCTGCACAAGGAGAACATGGCTTCTCGACCTATTCTTGAGATCAGCCTGGGTCTGGCCAGAGATGTAATCAACCTTCACAACCGATATGAAGATCTCAAACCCTCCTTTAGTGCTTTTGAATCCTGCAAGGCTACGCGTGAAGCCAAGCTGGTAGAATATCAGAAGCAGAAGTTAGAACTGGATGCATTGGTTGCAGACTACAAGGAGACTAAATCCGCCGCTGATAAGCTAGAAAAGGACATTGAGAACCTCCATAAGCAACTAGTTGTACTAAGGGAGAGGCAGAAGAATCTTAGAGCTGGATTGGGTGCGAAGACCAAGTCTAGTTTCCTTTTGCAAAGCATGGTCGCAACTTCAAGGCATGCACTAGAGATTGCAGAGGCCTCCATTCATCAAGGAGTGCTTCTCCAGTTTGAGATCTCCGTTAAGAAGGCTGGATTGCAAGAGACTCTCAAGAAACTAGGCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1328

Amino Acids

146.38

Weight (kDa)

6.25

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2765
Acc16I TGCGCA 1 cut(s) 1790
Acc36I ACCTGC 4 cut(s) 290, 434, 1186, 3519
AccB7I CCANNNNNTGG 3 cut(s) 974, 2087, 2756
AccII CGCG 3 cut(s) 83, 1881, 3649
AclI AACGTT 1 cut(s) 2619
AclWI GGATC 9 cut(s) 237, 381, 2117, 2130, 2671, 3160, 3375, 3388, 3468
AcoI YGGCCR 5 cut(s) 343, 487, 743, 2830, 3567
AcsI RAATTY 5 cut(s) 522, 771, 1751, 1928, 2390
AcuI CTGAAG 4 cut(s) 303, 447, 2034, 2937
AcvI CACGTG 1 cut(s) 483
AcyI GRCGYC 1 cut(s) 3042
AfaI GTAC 6 cut(s) 11, 758, 2184, 2576, 2791, 3783
AflII CTTAAG 1 cut(s) 1808
AflIII ACRYGT 3 cut(s) 482, 2289, 3647
AhlI ACTAGT 3 cut(s) 779, 1465, 3775
AjnI CCWGG 7 cut(s) 256, 400, 2134, 2958, 3283, 3345, 3558
AjuI GAANNNNNNNTTGG 2 cut(s) 3824, 3856
AloI GAACNNNNNNTCC 2 cut(s) 2731, 2763
Alw21I GWGCWC 1 cut(s) 3183
Alw26I GTCTC 7 cut(s) 556, 1178, 1484, 2298, 2662, 3713, 3953
AlwI GGATC 9 cut(s) 237, 381, 2117, 2130, 2671, 3160, 3375, 3388, 3468
AlwNI CAGNNNCTG 2 cut(s) 2414, 2666
Ama87I CYCGRG 1 cut(s) 1965
ApeKI GCWGC 3 cut(s) 206, 3358, 3514
ApoI RAATTY 5 cut(s) 522, 771, 1751, 1928, 2390
ArsI GACNNNNNNTTYG 6 cut(s) 1702, 1734, 2265, 2297, 3818, 3850
AseI ATTAAT 3 cut(s) 683, 1448, 1998
Asp700I GAANNNNTTC 3 cut(s) 20, 57, 177
AspA2I CCTAGG 1 cut(s) 3037
AspLEI GCGC 4 cut(s) 704, 1791, 1883, 2133
AspS9I GGNCC 8 cut(s) 138, 958, 1710, 2424, 2772, 3231, 3343, 3508
AsuC2I CCSGG 2 cut(s) 636, 3029
AsuHPI GGTGA 9 cut(s) 248, 305, 392, 449, 1040, 1126, 2210, 2344, 2408
AsuNHI GCTAGC 1 cut(s) 3101
AvaI CYCGRG 1 cut(s) 1965
AvaII GGWCC 6 cut(s) 138, 958, 1710, 3231, 3343, 3508
AvrII CCTAGG 1 cut(s) 3037
BaeGI GKGCMC 1 cut(s) 1465
BalI TGGCCA 5 cut(s) 345, 489, 745, 2832, 3569
BamHI GGATCC 2 cut(s) 2122, 3380
BarI GAAGNNNNNNTAC 2 cut(s) 3570, 3602
BbrPI CACGTG 1 cut(s) 483
BbsI GAAGAC 2 cut(s) 1317, 3833
Bbv12I GWGCWC 1 cut(s) 3183
BbvI GCAGC 3 cut(s) 218, 3345, 3501
BceAI ACGGC 4 cut(s) 62, 1769, 2694, 2967
BcgI CGANNNNNNTGC 4 cut(s) 1763, 1797, 2420, 2454
BciT130I CCWGG 7 cut(s) 258, 402, 2136, 2960, 3285, 3347, 3560
BciVI GTATCC 1 cut(s) 986
BcnI CCSGG 2 cut(s) 636, 3029
BcoDI GTCTC 7 cut(s) 556, 1178, 1484, 2298, 2662, 3713, 3953
BcuI ACTAGT 3 cut(s) 779, 1465, 3775
BfmI CTRYAG 6 cut(s) 297, 441, 820, 1193, 2541, 3359
BfrI CTTAAG 1 cut(s) 1808
BfuAI ACCTGC 4 cut(s) 290, 434, 1186, 3519
BfuI GTATCC 1 cut(s) 986
BglII AGATCT 3 cut(s) 3502, 3604, 3930
BisI GCNGC 6 cut(s) 207, 1418, 2239, 3359, 3515, 3732
BlnI CCTAGG 1 cut(s) 3037
BlpI GCTNAGC 3 cut(s) 132, 1421, 3354
BlsI GCNGC 6 cut(s) 208, 1419, 2240, 3360, 3516, 3733
Bme1390I CCNGG 9 cut(s) 258, 402, 636, 2136, 2960, 3029, 3285, 3347, 3560
Bme18I GGWCC 6 cut(s) 138, 958, 1710, 3231, 3343, 3508
BmeT110I CYCGRG 1 cut(s) 1965
BmgT120I GGNCC 8 cut(s) 138, 958, 1710, 2424, 2772, 3231, 3343, 3508
BmiI GGNNCC 6 cut(s) 110, 959, 2124, 2425, 3203, 3382
BmrFI CCNGG 9 cut(s) 258, 402, 636, 2136, 2960, 3029, 3285, 3347, 3560
BmrI ACTGGG 1 cut(s) 2744
BmtI GCTAGC 1 cut(s) 3105
BmuI ACTGGG 1 cut(s) 2744
BpiI GAAGAC 2 cut(s) 1317, 3833
BplI GAGNNNNNCTC 2 cut(s) 3903, 3935
BpmI CTGGAG 2 cut(s) 1659, 3905
Bpu1102I GCTNAGC 3 cut(s) 132, 1421, 3354
BpuEI CTTGAG 5 cut(s) 2041, 2318, 3167, 3569, 3950
BpuMI CCSGG 2 cut(s) 636, 3029
Bsa29I ATCGAT 2 cut(s) 241, 385
BsaAI YACGTR 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 3042
BsaI GGTCTC 1 cut(s) 1178
BsaXI ACNNNNNCTCC 6 cut(s) 924, 954, 1938, 1968, 2731, 2761
Bse118I RCCGGY 1 cut(s) 1039
Bse1I ACTGG 6 cut(s) 2200, 2419, 2605, 2739, 3696, 3922
BseBI CCWGG 7 cut(s) 258, 402, 2136, 2960, 3285, 3347, 3560
BseCI ATCGAT 2 cut(s) 241, 385
BseNI ACTGG 6 cut(s) 2200, 2419, 2605, 2739, 3696, 3922
BseRI GAGGAG 1 cut(s) 1188
BseSI GKGCMC 1 cut(s) 1465
BseXI GCAGC 3 cut(s) 218, 3345, 3501
BsgI GTGCAG 3 cut(s) 1109, 2618, 3500
Bsh1236I CGCG 3 cut(s) 83, 1881, 3649
Bsh1285I CGRYCG 1 cut(s) 87
BshVI ATCGAT 2 cut(s) 241, 385
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 3183
BsiHKCI CYCGRG 1 cut(s) 1965
BsiSI CCGG 3 cut(s) 636, 1040, 3028
BslFI GGGAC 5 cut(s) 971, 1546, 1738, 2577, 3266
BsmAI GTCTC 7 cut(s) 556, 1178, 1484, 2298, 2662, 3713, 3953
BsmFI GGGAC 5 cut(s) 971, 1546, 1738, 2577, 3266
BsmI GAATGC 3 cut(s) 309, 453, 2240
Bso31I GGTCTC 1 cut(s) 1178
BsoBI CYCGRG 1 cut(s) 1965
Bsp1286I GDGCHC 2 cut(s) 1465, 3183
Bsp1407I TGTACA 2 cut(s) 756, 2574
Bsp1720I GCTNAGC 3 cut(s) 132, 1421, 3354
BspDI ATCGAT 2 cut(s) 241, 385
BspFNI CGCG 3 cut(s) 83, 1881, 3649
BspLI GGNNCC 6 cut(s) 110, 959, 2124, 2425, 3203, 3382
BspMAI CTGCAG 4 cut(s) 301, 445, 1197, 3363
BspMI ACCTGC 4 cut(s) 290, 434, 1186, 3519
BspOI GCTAGC 1 cut(s) 3105
BspPI GGATC 9 cut(s) 237, 381, 2117, 2130, 2671, 3160, 3375, 3388, 3468
BspTI CTTAAG 1 cut(s) 1808
BspTNI GGTCTC 1 cut(s) 1178
BsrFI RCCGGY 1 cut(s) 1039
BsrGI TGTACA 2 cut(s) 756, 2574
BsrI ACTGG 6 cut(s) 2200, 2419, 2605, 2739, 3696, 3922
BssAI RCCGGY 1 cut(s) 1039
BssNI GRCGYC 1 cut(s) 3042
BssT1I CCWWGG 4 cut(s) 2367, 2805, 2857, 3037
Bst2UI CCWGG 7 cut(s) 258, 402, 2136, 2960, 3285, 3347, 3560
Bst4CI ACNGT 6 cut(s) 828, 1123, 2182, 2614, 3058, 3109
Bst6I CTCTTC 2 cut(s) 1050, 2065
BstACI GRCGYC 1 cut(s) 3042
BstAFI CTTAAG 1 cut(s) 1808
BstAPI GCANNNNNTGC 1 cut(s) 3704
BstAUI TGTACA 2 cut(s) 756, 2574
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 5 cut(s) 204, 3103, 3122, 3164, 3876
BstENI CCTNNNNNAGG 1 cut(s) 1658
BstFNI CGCG 3 cut(s) 83, 1881, 3649
BstHHI GCGC 4 cut(s) 704, 1791, 1883, 2133
BstMAI GTCTC 7 cut(s) 556, 1178, 1484, 2298, 2662, 3713, 3953
BstMCI CGRYCG 1 cut(s) 87
BstNI CCWGG 7 cut(s) 258, 402, 2136, 2960, 3285, 3347, 3560
BstNSI RCATGY 7 cut(s) 757, 1488, 2293, 2655, 3019, 3166, 3878
BstSCI CCNGG 9 cut(s) 256, 400, 634, 2134, 2958, 3027, 3283, 3345, 3558
BstSFI CTRYAG 6 cut(s) 297, 441, 820, 1193, 2541, 3359
BstSLI GKGCMC 1 cut(s) 1465
BstUI CGCG 3 cut(s) 83, 1881, 3649
BstV1I GCAGC 3 cut(s) 218, 3345, 3501
BstV2I GAAGAC 2 cut(s) 1317, 3833
BstX2I RGATCY 7 cut(s) 2122, 2663, 3380, 3460, 3502, 3604, 3930
BstXI CCANNNNNNTGG 2 cut(s) 3120, 3457
BstYI RGATCY 7 cut(s) 2122, 2663, 3380, 3460, 3502, 3604, 3930
Bsu15I ATCGAT 2 cut(s) 241, 385
BsuI GTATCC 1 cut(s) 986
BsuTUI ATCGAT 2 cut(s) 241, 385
BtgZI GCGATG 1 cut(s) 2433
BtsI GCAGTG 5 cut(s) 209, 1190, 1419, 2415, 3417
BveI ACCTGC 4 cut(s) 290, 434, 1186, 3519
Cac8I GCNNGC 5 cut(s) 204, 3103, 3122, 3164, 3876
CaiI CAGNNNCTG 2 cut(s) 2414, 2666
CfoI GCGC 4 cut(s) 704, 1791, 1883, 2133
Cfr10I RCCGGY 1 cut(s) 1039
Cfr13I GGNCC 8 cut(s) 138, 958, 1710, 2424, 2772, 3231, 3343, 3508
ClaI ATCGAT 2 cut(s) 241, 385
CpoI CGGWCCG 2 cut(s) 138, 3231
CseI GACGC 3 cut(s) 2156, 2441, 3031
Csp6I GTAC 6 cut(s) 10, 757, 2183, 2575, 2790, 3782
CspI CGGWCCG 2 cut(s) 138, 3231
CviQI GTAC 6 cut(s) 10, 757, 2183, 2575, 2790, 3782
DraI TTTAAA 1 cut(s) 1942
EaeI YGGCCR 5 cut(s) 343, 487, 743, 2830, 3567
Eam1104I CTCTTC 2 cut(s) 1050, 2065
EarI CTCTTC 2 cut(s) 1050, 2065
EciI GGCGGA 1 cut(s) 3718
Eco130I CCWWGG 4 cut(s) 2367, 2805, 2857, 3037
Eco147I AGGCCT 4 cut(s) 359, 503, 3896, 3978
Eco31I GGTCTC 1 cut(s) 1178
Eco47I GGWCC 6 cut(s) 138, 958, 1710, 3231, 3343, 3508
Eco57I CTGAAG 4 cut(s) 303, 447, 2034, 2937
Eco72I CACGTG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 1965
EcoNI CCTNNNNNAGG 1 cut(s) 1658
EcoO109I RGGNCCY 2 cut(s) 1710, 2772
EcoRI GAATTC 1 cut(s) 522
EcoRII CCWGG 7 cut(s) 256, 400, 2134, 2958, 3283, 3345, 3558
EcoT14I CCWWGG 4 cut(s) 2367, 2805, 2857, 3037
EcoT22I ATGCAT 3 cut(s) 372, 516, 3700
ErhI CCWWGG 4 cut(s) 2367, 2805, 2857, 3037
FalI AAGNNNNNCTT 4 cut(s) 2351, 2383, 3900, 3932
FaqI GGGAC 5 cut(s) 971, 1546, 1738, 2577, 3266
Fnu4HI GCNGC 6 cut(s) 207, 1418, 2239, 3359, 3515, 3732
Fsp4HI GCNGC 6 cut(s) 207, 1418, 2239, 3359, 3515, 3732
FspI TGCGCA 1 cut(s) 1790
GlaI GCGC 4 cut(s) 703, 1790, 1882, 2132
GluI GCNGC 6 cut(s) 207, 1418, 2239, 3359, 3515, 3732
GsuI CTGGAG 2 cut(s) 1659, 3905
HapII CCGG 3 cut(s) 636, 1040, 3028
HgaI GACGC 3 cut(s) 2156, 2441, 3031
HhaI GCGC 4 cut(s) 704, 1791, 1883, 2133
Hin1I GRCGYC 1 cut(s) 3042
Hin6I GCGC 4 cut(s) 702, 1789, 1881, 2131
HinP1I GCGC 4 cut(s) 702, 1789, 1881, 2131
HincII GTYRAC 4 cut(s) 1257, 1561, 2610, 2617
HindII GTYRAC 4 cut(s) 1257, 1561, 2610, 2617
HindIII AAGCTT 3 cut(s) 158, 1018, 2794
HpaI GTTAAC 2 cut(s) 1561, 2617
HpaII CCGG 3 cut(s) 636, 1040, 3028
HphI GGTGA 9 cut(s) 248, 305, 392, 449, 1040, 1126, 2210, 2344, 2408
Hpy166II GTNNAC 9 cut(s) 1257, 1561, 1760, 1877, 2575, 2610, 2617, 3427, 3450
Hpy8I GTNNAC 9 cut(s) 1257, 1561, 1760, 1877, 2575, 2610, 2617, 3427, 3450
Hpy99I CGWCG 6 cut(s) 83, 122, 2432, 2435, 3047, 3215
HpyCH4III ACNGT 6 cut(s) 828, 1123, 2182, 2614, 3058, 3109
HpyCH4IV ACGT 4 cut(s) 117, 482, 2566, 2619
HpySE526I ACGT 4 cut(s) 117, 482, 2566, 2619
Hsp92I GRCGYC 1 cut(s) 3042
HspAI GCGC 4 cut(s) 702, 1789, 1881, 2131
KspAI GTTAAC 2 cut(s) 1561, 2617
LmnI GCTCC 3 cut(s) 1640, 2440, 3092
Lsp1109I GCAGC 3 cut(s) 218, 3345, 3501
MaeII ACGT 4 cut(s) 117, 482, 2566, 2619
MfeI CAATTG 2 cut(s) 951, 2687
MflI RGATCY 7 cut(s) 2122, 2663, 3380, 3460, 3502, 3604, 3930
MhlI GDGCHC 2 cut(s) 1465, 3183
MlsI TGGCCA 5 cut(s) 345, 489, 745, 2832, 3569
MluI ACGCGT 1 cut(s) 3647
MluNI TGGCCA 5 cut(s) 345, 489, 745, 2832, 3569
MlyI GAGTC 4 cut(s) 1022, 1745, 2051, 3955
MmeI TCCRAC 1 cut(s) 910
Mox20I TGGCCA 5 cut(s) 345, 489, 745, 2832, 3569
Mph1103I ATGCAT 3 cut(s) 372, 516, 3700
MroXI GAANNNNTTC 3 cut(s) 20, 57, 177
MscI TGGCCA 5 cut(s) 345, 489, 745, 2832, 3569
MslI CAYNNNNRTG 5 cut(s) 318, 462, 1866, 2708, 3008
Msp20I TGGCCA 5 cut(s) 345, 489, 745, 2832, 3569
MspA1I CMGCKG 4 cut(s) 971, 1420, 3358, 3734
MspCI CTTAAG 1 cut(s) 1808
MspI CCGG 3 cut(s) 636, 1040, 3028
MspR9I CCNGG 9 cut(s) 258, 402, 636, 2136, 2960, 3029, 3285, 3347, 3560
MunI CAATTG 2 cut(s) 951, 2687
Mva1269I GAATGC 3 cut(s) 309, 453, 2240
MvaI CCWGG 7 cut(s) 258, 402, 2136, 2960, 3285, 3347, 3560
MvnI CGCG 3 cut(s) 83, 1881, 3649
NciI CCSGG 2 cut(s) 636, 3029
NheI GCTAGC 1 cut(s) 3101
NlaIV GGNNCC 6 cut(s) 110, 959, 2124, 2425, 3203, 3382
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 7 cut(s) 815, 880, 1114, 2191, 2198, 2410, 2984
NsbI TGCGCA 1 cut(s) 1790
NsiI ATGCAT 3 cut(s) 372, 516, 3700
NspI RCATGY 7 cut(s) 757, 1488, 2293, 2655, 3019, 3166, 3878
PaeI GCATGC 2 cut(s) 3166, 3878
PasI CCCWGGG 1 cut(s) 2135
PceI AGGCCT 4 cut(s) 359, 503, 3896, 3978
PciI ACATGT 1 cut(s) 2289
PcsI WCGNNNNNNNCGW 1 cut(s) 3219
PctI GAATGC 3 cut(s) 309, 453, 2240
PdmI GAANNNNTTC 3 cut(s) 20, 57, 177
PfeI GAWTC 6 cut(s) 40, 178, 1949, 2326, 3632, 3802
PflFI GACNNNGTC 1 cut(s) 3832
PflMI CCANNNNNTGG 3 cut(s) 974, 2087, 2756
PfoI TCCNGGA 1 cut(s) 634
PkrI GCNGC 6 cut(s) 208, 1419, 2240, 3360, 3516, 3733
PleI GAGTC 4 cut(s) 1021, 1744, 2051, 3955
PmaCI CACGTG 1 cut(s) 483
PmlI CACGTG 1 cut(s) 483
PpsI GAGTC 4 cut(s) 1021, 1744, 2051, 3955
Ppu21I YACGTR 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 1710
PscI ACATGT 1 cut(s) 2289
PshBI ATTAAT 3 cut(s) 683, 1448, 1998
PsiI TTATAA 1 cut(s) 2765
Psp1406I AACGTT 1 cut(s) 2619
Psp5II RGGWCCY 1 cut(s) 1710
Psp6I CCWGG 7 cut(s) 256, 400, 2134, 2958, 3283, 3345, 3558
PspCI CACGTG 1 cut(s) 483
PspGI CCWGG 7 cut(s) 256, 400, 2134, 2958, 3283, 3345, 3558
PspN4I GGNNCC 6 cut(s) 110, 959, 2124, 2425, 3203, 3382
PspPI GGNCC 8 cut(s) 138, 958, 1710, 2424, 2772, 3231, 3343, 3508
PspPPI RGGWCCY 1 cut(s) 1710
PstI CTGCAG 4 cut(s) 301, 445, 1197, 3363
PstNI CAGNNNCTG 2 cut(s) 2414, 2666
PsuI RGATCY 7 cut(s) 2122, 2663, 3380, 3460, 3502, 3604, 3930
PsyI GACNNNGTC 1 cut(s) 3832
PvuII CAGCTG 2 cut(s) 971, 3358
RsaI GTAC 6 cut(s) 11, 758, 2184, 2576, 2791, 3783
RsaNI GTAC 6 cut(s) 10, 757, 2183, 2575, 2790, 3782
RseI CAYNNNNRTG 5 cut(s) 318, 462, 1866, 2708, 3008
Rsr2I CGGWCCG 2 cut(s) 138, 3231
RsrII CGGWCCG 2 cut(s) 138, 3231
SatI GCNGC 6 cut(s) 207, 1418, 2239, 3359, 3515, 3732
Sau96I GGNCC 8 cut(s) 138, 958, 1710, 2424, 2772, 3231, 3343, 3508
SchI GAGTC 4 cut(s) 1022, 1745, 2051, 3955
ScrFI CCNGG 9 cut(s) 258, 402, 636, 2136, 2960, 3029, 3285, 3347, 3560
SduI GDGCHC 2 cut(s) 1465, 3183
SfcI CTRYAG 6 cut(s) 297, 441, 820, 1193, 2541, 3359
SinI GGWCC 6 cut(s) 138, 958, 1710, 3231, 3343, 3508
SmiMI CAYNNNNRTG 5 cut(s) 318, 462, 1866, 2708, 3008
SmlI CTYRAG 6 cut(s) 1808, 2020, 2297, 3182, 3548, 3965
SmoI CTYRAG 6 cut(s) 1808, 2020, 2297, 3182, 3548, 3965
SpeI ACTAGT 3 cut(s) 779, 1465, 3775
SphI GCATGC 2 cut(s) 3166, 3878
SseBI AGGCCT 4 cut(s) 359, 503, 3896, 3978
SspI AATATT 2 cut(s) 1620, 1804
StuI AGGCCT 4 cut(s) 359, 503, 3896, 3978
StyD4I CCNGG 9 cut(s) 256, 400, 634, 2134, 2958, 3027, 3283, 3345, 3558
StyI CCWWGG 4 cut(s) 2367, 2805, 2857, 3037
TaaI ACNGT 6 cut(s) 828, 1123, 2182, 2614, 3058, 3109
TaiI ACGT 4 cut(s) 120, 485, 2569, 2622
TaqI TCGA 7 cut(s) 24, 181, 241, 362, 385, 2067, 3538
TaqII GACCGA 1 cut(s) 2331
TatI WGTACW 3 cut(s) 756, 2574, 3781
TauI GCSGC 3 cut(s) 1420, 2241, 3734
TfiI GAWTC 6 cut(s) 40, 178, 1949, 2326, 3632, 3802
TseFI GTSAC 7 cut(s) 815, 880, 1114, 2191, 2198, 2410, 2984
TseI GCWGC 3 cut(s) 206, 3358, 3514
Tsp45I GTSAC 7 cut(s) 815, 880, 1114, 2191, 2198, 2410, 2984
TspGWI ACGGA 2 cut(s) 638, 3925
Tth111I GACNNNGTC 1 cut(s) 3832
Van91I CCANNNNNTGG 3 cut(s) 974, 2087, 2756
Vha464I CTTAAG 1 cut(s) 1808
VpaK11BI GGWCC 6 cut(s) 138, 958, 1710, 3231, 3343, 3508
VspI ATTAAT 3 cut(s) 683, 1448, 1998
XagI CCTNNNNNAGG 1 cut(s) 1658
XapI RAATTY 5 cut(s) 522, 771, 1751, 1928, 2390
XbaI TCTAGA 1 cut(s) 2923
XceI RCATGY 7 cut(s) 757, 1488, 2293, 2655, 3019, 3166, 3878
XcmI CCANNNNNNNNNTGG 3 cut(s) 919, 950, 1549
XmaJI CCTAGG 1 cut(s) 3037
XmnI GAANNNNTTC 3 cut(s) 20, 57, 177
Zsp2I ATGCAT 3 cut(s) 372, 516, 3700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.