MD12G1080800.v1.1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002172
Physical Location & Seq
Forward (+)
9888618 .. 9904704
16087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1080800.v1.1.491

Sequence Viewer

Length: 4353 bp
ATGGAGAAGTTCTTGAAGGACGTCGAGGTGGAGTACAAGAATCCATCGGAGGAGGCTATAAGGAGATGGAGAAGCGCCGTCGCGCTCGTCAAAAACCCTCGCAGAAGGTTTCGATTAGTCCCCGATCTCGCCAAGCGCTCCGAGGCCGAAAAGAAGAAGCTTCAAATCCAGGAAAAGATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTGCAGTTCATTGATGCTGGTGATCGTGGATCTATTGAAAAGCCAGGTCATGATGAACTCCAGCTCCCAGAAGATGCCAGAATGGCAGGTTTTAGCATTCACCCAGATGAACTTGCATCTATTACACGTGCCCATGATATTAAGGCCTTGGAAAGCCATGGTGGAATTCATGGGATTTTAAGGAAAGTCAATGTCTCGGTAGATGAAGGTGTCAAGGATAGTAATATACCAATTAGACAAAATGTTTATGGCTTAAACCGTTACAAGGAGAAACCTCCCCGAACTTTTTGGGTGTTTGTCTGGGAAGCACTACAGGACTTAACACTAATGATCCTTATGGTCTGTGCTGTGGTTTCTATTGGAGTTGGAATTGCCACTGAAAGCTGGCCCAAAGGCACGTACAATGGTCTGGGAATTTTAATTAGTATAATTCTAGTGGTTATGGTTACCGCCATTAGTGACTACAAGCAGTCTTTGCAATTCAAGGATTTGGATAGGGAGAAGAAAAAGATTTTTGTTCAGGTCACTAGGGATGGAAAAAGACAAAAAGTTTCCATTTACGACTTGGTTATTGGAGATATTGTGCTTTTGTTGATTGGGGACCAAGTTCCAGCTGATGGACTTTTCATATCTGGGTACAGTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGAATGTATCTGAAGGGAAGCCTTTTCTTCTTTCCGGAACTAAAGTGCAGGATGGGTCATGTAAAATGCTAGTGACTACAGTTGGTATGAAGACTGAATGGGGAAAGTTGATGGAAACTCTGAGTGAAGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTTAATGGTGTCGCTACAATTATTGGTAAAATTGGTTTGACTTTTGCAGTGTTGACATTTTTGGTATTGACAGTAAGATTTTTGGTGACAAAAGGACCTAACAACGAGATCACTGATTGGTCTTCAACTGATGCCGTAACCCTTTTGAGCTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACCATTAGCAGTGACGCTGAGCCTTGCTTTTGCAATGAAAAAATTGATGAATGACAGGGCACTTGTAAGGCATCTCTCAGCATGTGAGACAATGGGTTCTGCTAGTTGTATTTGCACAGATAAAACTGGAACATTAACTACAAATCATATGGTAGTTACCAAAGTATGGATGTGTGAAAAATCTGTAGATGTAAAAGAAAATGACAGTAAAGAAACATTGATATCAGAAATATCTGGAGCATCAAGCATCCTTTTGCAGGTTATATTCCAAAATACAAGTTCTGAGGTTATTAAGGATGATGGAAAGATCTCCATTTTGGGAACACCAACAGAGTCAGCACTATTAGAGTTCGGTTTACTTTTAGGTGGGGATTTTGATACCCTGCGCAGAGAGGTTAAGATTCTTAAGATCAGACCTTTCAATTCTGTCAGGAAGAAAATCTCTGTGCTTGTAGCTTATCCTCATGGTGGAACACGAGCTTTTTGCAAAGGTGCATCAGAAATAGTACTGGGAATATGTAACAAGTACATTGACTCTAATGGGGAATCTGTTCATCTCTCCAGAGAAATGGTAAAGAATATCACGGATGTCATAAATTCTTTTGCCTGTGAAGCATTGAGAACTCTCTGCTTAGCTTTTAAGGATATAGATGACTCTTCCATCGAAAATGGCATCCCAGATGATGGCTATACATTGGTAGCAGTTGTCGGTATTAACGACCCTGTGAGCCCGGGGGTCAGGGAGGCAGTTCAGACTTGTTTAGCTGCTGGAATCACTGGTGACAATATAAATACAGCTAAAGCCATCGCTAAAGAATGTGGCATACTCACAGAGGGTTGTATAGCAATTGAAGGACCTGAGTTTCGTAGCATGTCTCTAGAGAAGATGAAAACTGTGATACCGAAGATACAGGTAATGGCCCGGTCTTTACCGTTGGATAAGCACACATTGGTAAAAAATTTAAGGGATGAATTTGGTGAGGTTGTTGCTGTGACTGGTGATGGGACTAACGACGCTCCCGCTTTGCATGAGTCAGACATTGGACTTGCTATGGGCATAGCAGGAATAGAGGTTGCCAAAGAAAATGCCGATGGCATCATATCGGATGACAATTTTAAAACTATAGTAAATGTGGCCAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTGCAGTTCCAGTTAACAGTTAACGTTGTTGCTCTAATGATCAATGTTGTTTCTGCATGTGTCTTAGGATATGCTCCCCTTACAGCGGTGCAACTGCTCTGGGTGAATATGATTATGGACACTCTTGGTGCTTTGGCACTGGCAACAGAGTCTCCAAATGATGGACTTATGAAAAGGCCCCTAGTTGGGAGGGGTACCAACTTCATCACCAAGGCTGTGTGGAGAAATATCATTGGTCAGAGTATCTATCAACTGGCTGTCCTTGGAGTTCTCAATTTCTCTGGGGAGAAGCTACTAGGACTAACTGGTTCAGATGCAACTGAGGTTCTCAACACTGTGATATTCAACGCATTCGTGTTTTGCCAGGTGTTTAATCAGATAAACAGTCGCGACATAGTAAAGATTAACGTATTCCATGGAATGTTCGACAGCTGGGTATTTCGAATCGTCATGGTTTGCACAGCAACCTTCCAGTGGGACCCAAAAACTCACAGGCCAGCAAGGCGAGAGCTTTCTCCGGTCGAGCAAAGGGCAGTGCCCAGAACACCCAGAGAGAGCTTTCCTGAAGCCAGTATGCAGACCAAGGCCAGCAAGGCAGGCAAGAGCTGGTCGAGCAAAGGGGCAGTGCCCAGAGCACCCTCAGAGAGCTCTCCTGACACCAATATGCAGATTGATATCTCCTCAGATTCCAATGGAACAACCTGTGCTGTCGTTCGGTATAAAAAAGGATTCCTTTTCGTTACTGATGGACGCCGCACAAAATCTCTAGAAAATGATGAAAGAAAAATCATCTGTGATGATGTCATAAAATTCAGGTATGTTTGCAAAGATCAGTTTGGACACGACATATGTTCTGTTTCGGCGGGATCGGTAAAAACGATAGGCCGTCTCTGGAAACGTGTGCTAGAGTGTGAATTGAGTCCTCGTACTGTTTCTGAAGTAGTAGATCTTGTTCAGAAGACACTGATGACAGTATACAGTCTGAAGGATTTCAAATCTGGTGAAACGTGTTCAATAATCATTGCTGGAGGACTTGGACAAGAGATTTTCCATGTTGGTGGTCGCTTAGCAAATATGCCTCATTATATAAGTTGCGCCTACCATGTCATAGGTTCAGGATCCCGATTAGTAAACGCTGCATTGGAAGTACTTTACTCTGATCAACATGATGAAGGGACACTTGTAAGGACTGTTGAGAAATCATATCTGTTTGCTAGTTTGCATGATCAATCTACAGGAGGGTTTATTAAATTTTATAATGTTCATGGTGAAATACATCAGCACCTATTCACTAAACACACTCTCGAATTATACAGAGACATATATGCAGATCATATTAAACCGGACGAGGTAGCGGTGCTTATCTTCTCTAAGGACTTTGATAAGAAGCGTATTCATAAGTGTTTCCACAGGGAGACAGTTTTGAGGCACGCTCATGTGTTGGGCTACGTGAGAGGCTATCAAGTGAATAGAGTGGTATTGAAAAGGACAGATTATGAGAACCACCTATGTACTGTTCGGTTTGTAAAAGAAAAGTTACTTAGCAGTGGATATGTACCTGAGTGTAAAGATTCCGTGATTAAAGATTTCAATTTTTCTTTTTACAAGCTGAGCCAAAAACATAGGAAAGGGCTTTACTATTTTACAGAGGTTTCTAGGGATATATTAAAGGATCTGGTTCCACAAGGGATGGTGGAAGGATTAATTGGGAGAAATGCATATCACCCTGGAAGAGAACAAAGTCTAGAGAATGTGGAAAGACGACTTGTGGTAAAAACTGGTCCTAAAGAATCCCCAGGGCTAGAGGATGTGCTGAATGAATTTCTTGGAGAAGAACCAATGCCAGAGGAAGTGCCAGGGGATGTTTATGATGAACTGGAAATCAATCAACTGGAGGTCCCTGCTCGTGCTGGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1451

Amino Acids

160.58

Weight (kDa)

6.71

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 9 - 48 2.5e-16 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 122 - 189 1.1e-13 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 242 - 338 7.8e-23 P-type ATPase actuator domain
Cation_ATPase PF13246 514 - 661 1.4e-27 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 637 - 763 3.8e-07 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 836 - 969 2.8e-28 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3690
AarI CACCTGC 1 cut(s) 1039
AatII GACGTC 1 cut(s) 24
Acc16I TGCGCA 1 cut(s) 1643
Acc36I ACCTGC 3 cut(s) 287, 1039, 1504
Acc65I GGTACC 1 cut(s) 2627
AccB1I GGYRCC 1 cut(s) 2627
AccB7I CCANNNNNTGG 4 cut(s) 827, 1422, 1940, 2594
AccI GTMKAC 1 cut(s) 3408
AccII CGCG 2 cut(s) 83, 2823
AccIII TCCGGA 1 cut(s) 929
AciI CCGC 7 cut(s) 660, 1220, 2277, 2519, 3187, 3296, 3788
AclI AACGTT 1 cut(s) 2457
AclWI GGATC 6 cut(s) 247, 535, 3307, 3546, 3559, 4113
AcoI YGGCCR 1 cut(s) 2391
AcsI RAATTY 8 cut(s) 375, 624, 1852, 2215, 2228, 3242, 3683, 4253
AcuI CTGAAG 4 cut(s) 927, 3018, 3390, 3437
AcvI CACGTG 1 cut(s) 338
AcyI GRCGYC 2 cut(s) 21, 3184
AfeI AGCGCT 1 cut(s) 137
AflII CTTAAG 1 cut(s) 1661
AflIII ACRYGT 3 cut(s) 335, 3331, 3440
AjnI CCWGG 6 cut(s) 168, 253, 2796, 4159, 4228, 4288
AjuI GAANNNNNNNTTGG 2 cut(s) 4122, 4154
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 2 cut(s) 3070, 3083
Alw26I GTCTC 8 cut(s) 409, 1031, 1337, 2136, 2589, 3326, 3744, 3842
AlwI GGATC 6 cut(s) 247, 535, 3307, 3546, 3559, 4113
Ama87I CYCGRG 1 cut(s) 1987
Aor13HI TCCGGA 1 cut(s) 929
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 9 cut(s) 144, 354, 596, 2175, 2391, 2609, 2927, 3018, 3316
ApeKI GCWGC 3 cut(s) 206, 2021, 3569
ApoI RAATTY 8 cut(s) 375, 624, 1852, 2215, 2228, 3242, 3683, 4253
ArsI GACNNNNNNTTYG 2 cut(s) 2103, 2135
AseI ATTAAT 1 cut(s) 4136
Asp700I GAANNNNTTC 3 cut(s) 177, 1806, 3422
Asp718I GGTACC 1 cut(s) 2627
AspLEI GCGC 5 cut(s) 77, 85, 138, 1644, 3530
AspS9I GGNCC 9 cut(s) 597, 811, 1151, 2111, 2176, 2610, 2911, 4214, 4330
AsuC2I CCSGG 3 cut(s) 1988, 1989, 2179
AsuII TTCGAA 1 cut(s) 2875
AvaI CYCGRG 1 cut(s) 1987
AvaII GGWCC 6 cut(s) 811, 1151, 2111, 2911, 4214, 4330
BaeGI GKGCMC 4 cut(s) 343, 1318, 2973, 3063
BalI TGGCCA 1 cut(s) 2393
BamHI GGATCC 1 cut(s) 3551
BanI GGYRCC 1 cut(s) 2627
BanII GRGCYC 2 cut(s) 1988, 3083
BauI CACGAG 2 cut(s) 1731, 4338
BbrPI CACGTG 1 cut(s) 338
BbsI GAAGAC 3 cut(s) 992, 1170, 3398
Bbv12I GWGCWC 2 cut(s) 3070, 3083
BbvI GCAGC 3 cut(s) 218, 2008, 3556
BceAI ACGGC 3 cut(s) 62, 1175, 3303
BcgI CGANNNNNNTGC 2 cut(s) 1722, 1756
BciT130I CCWGG 6 cut(s) 170, 255, 2798, 4161, 4230, 4290
BclI TGATCA 3 cut(s) 2472, 3592, 3658
BcnI CCSGG 3 cut(s) 1988, 1989, 2179
BcoDI GTCTC 8 cut(s) 409, 1031, 1337, 2136, 2589, 3326, 3744, 3842
BfmI CTRYAG 7 cut(s) 212, 521, 972, 1046, 1440, 2379, 3666
BfoI RGCGCY 2 cut(s) 78, 139
BfrI CTTAAG 1 cut(s) 1661
BfuAI ACCTGC 3 cut(s) 287, 1039, 1504
BglI GCCNNNNNGGC 3 cut(s) 293, 2935, 3026
BglII AGATCT 2 cut(s) 1563, 3379
BisI GCNGC 4 cut(s) 207, 2022, 3187, 3570
BlpI GCTNAGC 4 cut(s) 1274, 1888, 3499, 4043
BlsI GCNGC 4 cut(s) 208, 2023, 3188, 3571
BmcAI AGTACT 2 cut(s) 1764, 3582
Bme1390I CCNGG 9 cut(s) 170, 255, 1988, 1989, 2179, 2798, 4161, 4230, 4290
Bme18I GGWCC 6 cut(s) 811, 1151, 2111, 2911, 4214, 4330
BmeT110I CYCGRG 1 cut(s) 1987
BmgT120I GGNCC 9 cut(s) 597, 811, 1151, 2111, 2176, 2610, 2911, 4214, 4330
BmiI GGNNCC 8 cut(s) 812, 2612, 2629, 2912, 2913, 3553, 4113, 4332
BmrFI CCNGG 9 cut(s) 170, 255, 1988, 1989, 2179, 2798, 4161, 4230, 4290
BmrI ACTGGG 1 cut(s) 1775
BmuI ACTGGG 1 cut(s) 1775
BpiI GAAGAC 3 cut(s) 992, 1170, 3398
BplI GAGNNNNNCTC 2 cut(s) 3850, 3882
BpmI CTGGAG 5 cut(s) 254, 1512, 1801, 3480, 4346
Bpu1102I GCTNAGC 4 cut(s) 1274, 1888, 3499, 4043
Bpu14I TTCGAA 1 cut(s) 2875
BpuMI CCSGG 3 cut(s) 1988, 1989, 2179
BsaAI YACGTR 3 cut(s) 338, 609, 3883
BsaBI GATNNNNATC 1 cut(s) 3107
BsaHI GRCGYC 2 cut(s) 21, 3184
BsaI GGTCTC 1 cut(s) 1031
BsaWI WCCGGW 3 cut(s) 929, 2950, 3775
BsaXI ACNNNNNCTCC 6 cut(s) 258, 288, 777, 807, 2569, 2599
Bse3DI GCAATG 2 cut(s) 1296, 3453
Bse8I GATNNNNATC 1 cut(s) 3107
BseAI TCCGGA 1 cut(s) 929
BseBI CCWGG 6 cut(s) 170, 255, 2798, 4161, 4230, 4290
BseJI GATNNNNATC 1 cut(s) 3107
BseMI GCAATG 2 cut(s) 1296, 3453
BseRI GAGGAG 3 cut(s) 65, 1041, 3103
BseSI GKGCMC 4 cut(s) 343, 1318, 2973, 3063
BseXI GCAGC 3 cut(s) 218, 2008, 3556
BseYI CCCAGC 1 cut(s) 2865
BsgI GTGCAG 2 cut(s) 962, 2456
Bsh1236I CGCG 2 cut(s) 83, 2823
Bsh1285I CGRYCG 1 cut(s) 2955
BshFI GGCC 9 cut(s) 146, 356, 598, 2177, 2393, 2611, 2929, 3020, 3318
BshNI GGYRCC 1 cut(s) 2627
BsiEI CGRYCG 1 cut(s) 2955
BsiHKAI GWGCWC 2 cut(s) 3070, 3083
BsiHKCI CYCGRG 1 cut(s) 1987
BsiSI CCGG 5 cut(s) 930, 1988, 2179, 2951, 3776
BslFI GGGAC 7 cut(s) 104, 824, 2275, 2415, 2924, 3622, 4316
BsmAI GTCTC 8 cut(s) 409, 1031, 1337, 2136, 2589, 3326, 3744, 3842
BsmBI CGTCTC 1 cut(s) 3326
BsmFI GGGAC 7 cut(s) 104, 824, 2275, 2415, 2924, 3622, 4316
BsmI GAATGC 2 cut(s) 306, 2783
BsnI GGCC 9 cut(s) 146, 356, 598, 2177, 2393, 2611, 2929, 3020, 3318
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 1 cut(s) 1987
Bsp119I TTCGAA 1 cut(s) 2875
Bsp1286I GDGCHC 7 cut(s) 343, 1318, 1988, 2973, 3063, 3070, 3083
Bsp13I TCCGGA 1 cut(s) 929
Bsp1407I TGTACA 1 cut(s) 2412
Bsp1720I GCTNAGC 4 cut(s) 1274, 1888, 3499, 4043
Bsp19I CCATGG 2 cut(s) 367, 2848
Bsp68I TCGCGA 1 cut(s) 2823
BspACI CCGC 7 cut(s) 660, 1220, 2277, 2519, 3187, 3296, 3788
BspANI GGCC 9 cut(s) 146, 356, 598, 2177, 2393, 2611, 2929, 3020, 3318
BspEI TCCGGA 1 cut(s) 929
BspFNI CGCG 2 cut(s) 83, 2823
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 8 cut(s) 812, 2612, 2629, 2912, 2913, 3553, 4113, 4332
BspMAI CTGCAG 2 cut(s) 216, 1050
BspMI ACCTGC 3 cut(s) 287, 1039, 1504
BspPI GGATC 6 cut(s) 247, 535, 3307, 3546, 3559, 4113
BspT104I TTCGAA 1 cut(s) 2875
BspT107I GGYRCC 1 cut(s) 2627
BspTI CTTAAG 1 cut(s) 1661
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 2 cut(s) 1296, 3453
BsrGI TGTACA 1 cut(s) 2412
BssNAI GTATAC 1 cut(s) 3409
BssNI GRCGYC 2 cut(s) 21, 3184
BssSI CACGAG 2 cut(s) 1731, 4338
BssT1I CCWWGG 6 cut(s) 357, 367, 2643, 2695, 2848, 3015
Bst1107I GTATAC 1 cut(s) 3409
Bst2BI CACGAG 2 cut(s) 1731, 4338
Bst2UI CCWGG 6 cut(s) 170, 255, 2798, 4161, 4230, 4290
Bst6I CTCTTC 2 cut(s) 1918, 4159
BstACI GRCGYC 2 cut(s) 21, 3184
BstAFI CTTAAG 1 cut(s) 1661
BstAPI GCANNNNNTGC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 2412
BstBAI YACGTR 3 cut(s) 338, 609, 3883
BstBI TTCGAA 1 cut(s) 2875
BstC8I GCNNGC 5 cut(s) 596, 2931, 3022, 3031, 3864
BstDSI CCRYGG 2 cut(s) 367, 2848
BstEII GGTNACC 1 cut(s) 655
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstFNI CGCG 2 cut(s) 83, 2823
BstH2I RGCGCY 2 cut(s) 78, 139
BstHHI GCGC 5 cut(s) 77, 85, 138, 1644, 3530
BstMAI GTCTC 8 cut(s) 409, 1031, 1337, 2136, 2589, 3326, 3744, 3842
BstMCI CGRYCG 1 cut(s) 2955
BstMWI GCNNNNNNNGC 8 cut(s) 293, 685, 1054, 1868, 2935, 3026, 3030, 3045
BstNI CCWGG 6 cut(s) 170, 255, 2798, 4161, 4230, 4290
BstNSI RCATGY 3 cut(s) 1341, 2131, 2493
BstPI GGTNACC 1 cut(s) 655
BstSCI CCNGG 9 cut(s) 168, 253, 1986, 1987, 2177, 2796, 4159, 4228, 4288
BstSFI CTRYAG 7 cut(s) 212, 521, 972, 1046, 1440, 2379, 3666
BstSLI GKGCMC 4 cut(s) 343, 1318, 2973, 3063
BstUI CGCG 2 cut(s) 83, 2823
BstV1I GCAGC 3 cut(s) 218, 2008, 3556
BstV2I GAAGAC 3 cut(s) 992, 1170, 3398
BstX2I RGATCY 5 cut(s) 239, 1563, 3379, 3551, 4105
BstXI CCANNNNNNTGG 2 cut(s) 1825, 3491
BstYI RGATCY 5 cut(s) 239, 1563, 3379, 3551, 4105
BstZ17I GTATAC 1 cut(s) 3409
BsuRI GGCC 9 cut(s) 146, 356, 598, 2177, 2393, 2611, 2929, 3020, 3318
BtgI CCRYGG 2 cut(s) 367, 2848
BtgZI GCGATG 1 cut(s) 2047
BtsI GCAGTG 7 cut(s) 209, 1043, 1110, 1272, 2973, 3063, 3985
BtuMI TCGCGA 1 cut(s) 2823
BveI ACCTGC 3 cut(s) 287, 1039, 1504
Cac8I GCNNGC 5 cut(s) 596, 2931, 3022, 3031, 3864
CciI TCATGA 1 cut(s) 259
CfoI GCGC 5 cut(s) 77, 85, 138, 1644, 3530
Cfr13I GGNCC 9 cut(s) 597, 811, 1151, 2111, 2176, 2610, 2911, 4214, 4330
Cfr9I CCCGGG 1 cut(s) 1987
CseI GACGC 3 cut(s) 1279, 2279, 3192
DraI TTTAAA 1 cut(s) 2374
EaeI YGGCCR 1 cut(s) 2391
Eam1104I CTCTTC 2 cut(s) 1918, 4159
EarI CTCTTC 2 cut(s) 1918, 4159
Ecl136II GAGCTC 1 cut(s) 3081
Eco130I CCWWGG 6 cut(s) 357, 367, 2643, 2695, 2848, 3015
Eco147I AGGCCT 1 cut(s) 356
Eco24I GRGCYC 2 cut(s) 1988, 3083
Eco31I GGTCTC 1 cut(s) 1031
Eco32I GATATC 2 cut(s) 1479, 3109
Eco47I GGWCC 6 cut(s) 811, 1151, 2111, 2911, 4214, 4330
Eco47III AGCGCT 1 cut(s) 137
Eco53kI GAGCTC 1 cut(s) 3081
Eco57I CTGAAG 4 cut(s) 927, 3018, 3390, 3437
Eco72I CACGTG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 1987
Eco91I GGTNACC 1 cut(s) 655
EcoICRI GAGCTC 1 cut(s) 3081
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 5 cut(s) 1151, 2111, 2610, 2911, 4330
EcoO65I GGTNACC 1 cut(s) 655
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 6 cut(s) 168, 253, 2796, 4159, 4228, 4288
EcoRV GATATC 2 cut(s) 1479, 3109
EcoT14I CCWWGG 6 cut(s) 357, 367, 2643, 2695, 2848, 3015
EcoT22I ATGCAT 1 cut(s) 4153
EcoT38I GRGCYC 2 cut(s) 1988, 3083
ErhI CCWWGG 6 cut(s) 357, 367, 2643, 2695, 2848, 3015
Esp3I CGTCTC 1 cut(s) 3326
FalI AAGNNNNNCTT 4 cut(s) 3150, 3182, 3597, 3629
FaqI GGGAC 7 cut(s) 104, 824, 2275, 2415, 2924, 3622, 4316
FauI CCCGC 2 cut(s) 2284, 3289
FauNDI CATATG 2 cut(s) 1404, 3281
FbaI TGATCA 3 cut(s) 2472, 3592, 3658
FblI GTMKAC 1 cut(s) 3408
Fnu4HI GCNGC 4 cut(s) 207, 2022, 3187, 3570
FriOI GRGCYC 2 cut(s) 1988, 3083
Fsp4HI GCNGC 4 cut(s) 207, 2022, 3187, 3570
FspI TGCGCA 1 cut(s) 1643
GlaI GCGC 5 cut(s) 76, 84, 137, 1643, 3529
GluI GCNGC 4 cut(s) 207, 2022, 3187, 3570
GsaI CCCAGC 1 cut(s) 2869
GsuI CTGGAG 5 cut(s) 254, 1512, 1801, 3480, 4346
HaeII RGCGCY 2 cut(s) 78, 139
HaeIII GGCC 9 cut(s) 146, 356, 598, 2177, 2393, 2611, 2929, 3020, 3318
HapII CCGG 5 cut(s) 930, 1988, 2179, 2951, 3776
HgaI GACGC 3 cut(s) 1279, 2279, 3192
HhaI GCGC 5 cut(s) 77, 85, 138, 1644, 3530
Hin1I GRCGYC 2 cut(s) 21, 3184
Hin6I GCGC 5 cut(s) 75, 83, 136, 1642, 3528
HinP1I GCGC 5 cut(s) 75, 83, 136, 1642, 3528
HincII GTYRAC 3 cut(s) 1110, 2448, 2455
HindII GTYRAC 3 cut(s) 1110, 2448, 2455
HindIII AAGCTT 3 cut(s) 158, 871, 1055
HpaI GTTAAC 2 cut(s) 2448, 2455
HpaII CCGG 5 cut(s) 930, 1988, 2179, 2951, 3776
Hpy166II GTNNAC 7 cut(s) 1110, 1613, 2413, 2448, 2455, 3409, 3565
Hpy8I GTNNAC 7 cut(s) 1110, 1613, 2413, 2448, 2455, 3409, 3565
Hpy99I CGWCG 3 cut(s) 26, 83, 2273
HpyCH4IV ACGT 9 cut(s) 21, 337, 608, 2404, 2457, 2841, 3331, 3440, 3882
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 685, 1054, 1868, 2935, 3026, 3030, 3045
HpySE526I ACGT 9 cut(s) 21, 337, 608, 2404, 2457, 2841, 3331, 3440, 3882
Hsp92I GRCGYC 2 cut(s) 21, 3184
HspAI GCGC 5 cut(s) 75, 83, 136, 1642, 3528
KflI GGGWCCC 1 cut(s) 2911
Kpn2I TCCGGA 1 cut(s) 929
KpnI GGTACC 1 cut(s) 2631
Ksp22I TGATCA 3 cut(s) 2472, 3592, 3658
KspAI GTTAAC 2 cut(s) 2448, 2455
LmnI GCTCC 5 cut(s) 143, 279, 1493, 2278, 2512
Lsp1109I GCAGC 3 cut(s) 218, 2008, 3556
MaeII ACGT 9 cut(s) 21, 337, 608, 2404, 2457, 2841, 3331, 3440, 3882
MfeI CAATTG 1 cut(s) 2103
MflI RGATCY 5 cut(s) 239, 1563, 3379, 3551, 4105
MhlI GDGCHC 7 cut(s) 343, 1318, 1988, 2973, 3063, 3070, 3083
MlsI TGGCCA 1 cut(s) 2393
MluNI TGGCCA 1 cut(s) 2393
MlyI GAGTC 7 cut(s) 875, 1598, 1784, 1904, 2297, 2591, 3361
MmeI TCCRAC 2 cut(s) 556, 2172
Mox20I TGGCCA 1 cut(s) 2393
Mph1103I ATGCAT 1 cut(s) 4153
MroI TCCGGA 1 cut(s) 929
MroXI GAANNNNTTC 3 cut(s) 177, 1806, 3422
MscI TGGCCA 1 cut(s) 2393
MslI CAYNNNNRTG 4 cut(s) 315, 3095, 3489, 3867
Msp20I TGGCCA 1 cut(s) 2393
MspA1I CMGCKG 3 cut(s) 824, 2519, 2865
MspCI CTTAAG 1 cut(s) 1661
MspI CCGG 5 cut(s) 930, 1988, 2179, 2951, 3776
MspR9I CCNGG 9 cut(s) 170, 255, 1988, 1989, 2179, 2798, 4161, 4230, 4290
MunI CAATTG 1 cut(s) 2103
Mva1269I GAATGC 2 cut(s) 306, 2783
MvaI CCWGG 6 cut(s) 170, 255, 2798, 4161, 4230, 4290
MvnI CGCG 2 cut(s) 83, 2823
MwoI GCNNNNNNNGC 8 cut(s) 293, 685, 1054, 1868, 2935, 3026, 3030, 3045
NciI CCSGG 3 cut(s) 1988, 1989, 2179
NcoI CCATGG 2 cut(s) 367, 2848
NdeI CATATG 2 cut(s) 1404, 3281
NlaIV GGNNCC 8 cut(s) 812, 2612, 2629, 2912, 2913, 3553, 4113, 4332
NmuCI GTSAC 7 cut(s) 668, 733, 967, 1141, 1267, 2036, 2248
NruI TCGCGA 1 cut(s) 2823
NsbI TGCGCA 1 cut(s) 1643
NsiI ATGCAT 1 cut(s) 4153
NspI RCATGY 3 cut(s) 1341, 2131, 2493
NspV TTCGAA 1 cut(s) 2875
PagI TCATGA 1 cut(s) 259
PaqCI CACCTGC 1 cut(s) 1039
PasI CCCWGGG 1 cut(s) 4229
PceI AGGCCT 1 cut(s) 356
PcsI WCGNNNNNNNCGW 2 cut(s) 1971, 3308
PctI GAATGC 2 cut(s) 306, 2783
PdmI GAANNNNTTC 3 cut(s) 177, 1806, 3422
PflMI CCANNNNNTGG 4 cut(s) 827, 1422, 1940, 2594
PfoI TCCNGGA 1 cut(s) 168
PkrI GCNGC 4 cut(s) 208, 2023, 3188, 3571
PleI GAGTC 7 cut(s) 874, 1597, 1784, 1904, 2296, 2590, 3360
PmaCI CACGTG 1 cut(s) 338
PmlI CACGTG 1 cut(s) 338
PpsI GAGTC 7 cut(s) 874, 1597, 1784, 1904, 2296, 2590, 3360
Ppu21I YACGTR 3 cut(s) 338, 609, 3883
PpuMI RGGWCCY 4 cut(s) 1151, 2111, 2911, 4330
PshBI ATTAAT 1 cut(s) 4136
PsiI TTATAA 1 cut(s) 3690
Psp124BI GAGCTC 1 cut(s) 3083
Psp1406I AACGTT 1 cut(s) 2457
Psp5II RGGWCCY 4 cut(s) 1151, 2111, 2911, 4330
Psp6I CCWGG 6 cut(s) 168, 253, 2796, 4159, 4228, 4288
PspCI CACGTG 1 cut(s) 338
PspEI GGTNACC 1 cut(s) 655
PspFI CCCAGC 1 cut(s) 2865
PspGI CCWGG 6 cut(s) 168, 253, 2796, 4159, 4228, 4288
PspN4I GGNNCC 8 cut(s) 812, 2612, 2629, 2912, 2913, 3553, 4113, 4332
PspPI GGNCC 9 cut(s) 597, 811, 1151, 2111, 2176, 2610, 2911, 4214, 4330
PspPPI RGGWCCY 4 cut(s) 1151, 2111, 2911, 4330
PsrI GAACNNNNNNTAC 4 cut(s) 1378, 1410, 3369, 3401
PstI CTGCAG 2 cut(s) 216, 1050
PsuI RGATCY 5 cut(s) 239, 1563, 3379, 3551, 4105
PvuII CAGCTG 2 cut(s) 824, 2865
RruI TCGCGA 1 cut(s) 2823
RseI CAYNNNNRTG 4 cut(s) 315, 3095, 3489, 3867
SacI GAGCTC 1 cut(s) 3083
SatI GCNGC 4 cut(s) 207, 2022, 3187, 3570
Sau96I GGNCC 9 cut(s) 597, 811, 1151, 2111, 2176, 2610, 2911, 4214, 4330
ScaI AGTACT 2 cut(s) 1764, 3582
SchI GAGTC 7 cut(s) 875, 1598, 1784, 1904, 2297, 2591, 3361
ScrFI CCNGG 9 cut(s) 170, 255, 1988, 1989, 2179, 2798, 4161, 4230, 4290
SduI GDGCHC 7 cut(s) 343, 1318, 1988, 2973, 3063, 3070, 3083
SfcI CTRYAG 7 cut(s) 212, 521, 972, 1046, 1440, 2379, 3666
SfuI TTCGAA 1 cut(s) 2875
SinI GGWCC 6 cut(s) 811, 1151, 2111, 2911, 4214, 4330
SmaI CCCGGG 1 cut(s) 1989
SmiMI CAYNNNNRTG 4 cut(s) 315, 3095, 3489, 3867
SmlI CTYRAG 1 cut(s) 1661
SmoI CTYRAG 1 cut(s) 1661
SseBI AGGCCT 1 cut(s) 356
SsiI CCGC 7 cut(s) 660, 1220, 2277, 2519, 3187, 3296, 3788
SstI GAGCTC 1 cut(s) 3083
StuI AGGCCT 1 cut(s) 356
StyD4I CCNGG 9 cut(s) 168, 253, 1986, 1987, 2177, 2796, 4159, 4228, 4288
StyI CCWWGG 6 cut(s) 357, 367, 2643, 2695, 2848, 3015
TaiI ACGT 9 cut(s) 24, 340, 611, 2407, 2460, 2844, 3334, 3443, 3885
TaqI TCGA 9 cut(s) 24, 112, 181, 1920, 2859, 2875, 2955, 3044, 3738
TatI WGTACW 6 cut(s) 33, 1762, 1782, 2412, 3580, 3944
TauI GCSGC 1 cut(s) 3189
TseFI GTSAC 7 cut(s) 668, 733, 967, 1141, 1267, 2036, 2248
TseI GCWGC 3 cut(s) 206, 2021, 3569
Tsp45I GTSAC 7 cut(s) 668, 733, 967, 1141, 1267, 2036, 2248
TspGWI ACGGA 2 cut(s) 1856, 3997
TspMI CCCGGG 1 cut(s) 1987
Van91I CCANNNNNTGG 4 cut(s) 827, 1422, 1940, 2594
Vha464I CTTAAG 1 cut(s) 1661
VpaK11BI GGWCC 6 cut(s) 811, 1151, 2111, 2911, 4214, 4330
VspI ATTAAT 1 cut(s) 4136
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 8 cut(s) 375, 624, 1852, 2215, 2228, 3242, 3683, 4253
XbaI TCTAGA 3 cut(s) 2134, 3199, 4177
XceI RCATGY 3 cut(s) 1341, 2131, 2493
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XmaI CCCGGG 1 cut(s) 1987
XmiI GTMKAC 1 cut(s) 3408
XmnI GAANNNNTTC 3 cut(s) 177, 1806, 3422
ZraI GACGTC 1 cut(s) 22
ZrmI AGTACT 2 cut(s) 1764, 3582
Zsp2I ATGCAT 1 cut(s) 4153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.