Rh3BG291700

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002172
Physical Location & Seq
Forward (+)
29229822 .. 29238157
8336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG291700.1

Sequence Viewer

Length: 2568 bp
ATGGTTTGTGCCGTGGTTTCAATTGGAGTGGGACTTGCTACTGACGGGTGGCCAGAAGGCATGTATGATGGGGTGGGAATTTTAATCAGTATAGCTTTAGTTGTTTTGGTCACTGCCATTAGTGATTACAGGCAGTCCATGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTATTCAGGTCACTAGGGATGGGAAAAGACAAAAAGTTTCCATTTATGATTTGCTGGTGGGAGATATTGTTCATTTGGCAGTGGGTGACCTTGTTCCAGCAGATGGACTTTTCATATCAGGATACAGCTTGTTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGAATGTGTATGAAGAAAAGCCTTTTCTTCTTTCGGGAACCAAAGTGCAAGATGGGTCAGGTAAAATGCTGGTAACAACAGTTGGTATGAGGACCGAATGGGGAAAGTTGATGGAAACTCTGAGTGAGGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGGCTTTCGCTGTGGTGACATTTTTGGTTTTGACGGTAAGATATTTGGTGGAAAAAGCACTTAGCAATGAGATCACAGATTGGTCTTCAACTGATGCTGTAACACTTTTGAACTACTTTGCCATTGCAGTAACTATAATTGTTGTTGCAGTTCCTGAAGGTTTACCATTGGCAGTAACACTGAGTCTTGCTTTTGCAATGAAGAAATTAATGAATGATAAGGCACTTGTGAGGCATCTCTCAGCCTGTGAGACAATGGGTTCTGCTAGTTGCATATGCACAGATAAAACAGGAACATTAACCACAAACCATATGGTAGTTACCAAGATATGGATAGGTGACAAATCTATATATGTAAAAGGTAATGAAAGTAAAGACGTAGTAAAATCAGAAATTTCTGGAGCACTGGACATCCTATTGCAGGTTATATTTCAAAATACAAGCTCTGAGGTTATCAAGGATGAGGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCATTATTAGAATTTGGTTTGCTTTTGGGTGGCGATTTTGATGCCCAGCGCAGAGAGTTTAAGATTATTAAGATGGAACCTTTCAATTCTGTTAGGAAGAAGATGTCTGTGCTCATCGCTCATCCTGATGGTGGGATAAGAGCTTTTTGCAAAGGTGCATCTGAAATAGTATTGGGAATGTGCAACAAAGTTATTGACTGTAATGGAGAATCTGTTGATCTCTCCCGAAAAGAGGCAAATAATATCACAGATGTCATAAATTCTTTTGCTTCTGAAGCATTAAGAACTCTCTGCTTGGCTTTTAAAGATATACATGACTCTTCCATCAACAATGACATCCCCAATGATGGCTATACATTGATAGCAGTTGTAGGAATCAAGGACCCTGTGCGTCCTGGGGTGAAGGAAGCAGTCCAAACTTGTTTGGCTGCTGGGATAACTGTACGAATGGTCACAGGTGACAATATAAATACCGCTAAAGCCATAGCTAGAGAATGTGGCATACTCACAGAGGATGGTTTGGCAATAGAAGGACCAGAATTTCGTAACATGTCTCCTGCAGATATGAAAGCTGTTATACCGAGAATTCAGGTGATGGCCCGATCCTTACCATTGGATAAGCACACCTTGGTGAAGAATTTGAGGAATACATTCCGGGAGGTAGTTGCAGTTACAGGTGATGGGACCAATGACGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGCACAGAGGTTGCTAAAGAAAGTGCTGATGTCATCGTATTGGATGACAATTTTAGTACTATAGTAAATGTGGCTAGATGGGGACGTTCAGTGTACATAAACATTCAAAAGTTTGTCCAGTTCCAGTTAACGGTTAACGTTGTTGCTCTGATGATCAATTTTGTTTCTGCATGTGTATCAGGAGATGCTCCCCTTACTGCTGTGCAATTACTCTGGGTCAACATGATTATGGACACTCTTGGTGCTTTGGCACTGGCTACAGAGCCTCCAAATGAAGGACTGATGAAAAGGCCCCCTGTTTCAAGGGGTACAAGCTTCATCACCAGGCCCATGTGGAGGAATATCATTGGTCAGAGCATTTATCAATTGGCTGTTCTTGGAGTTCTCGATTTCCGTGGAACGCAGCTGCTAGGACTCACTGGTTCAGATGCAACTGATATTCTTGACACTGTGATATTCAATGCATTCGTGTTCTGCCAGGTGTTCAATCAGATAAACAGCCGTGATATAGAGAAGATAAACATATTCCGTGGAATGTTTGACAGCTGGGTATTCCTAGGGGTCATGGTTTGCACAGTGGGCTTCCAATTTGTCCTAGTAGAGTTCTTGGGCGCGTTTGCTAGCACTGTACCACTGAGCTGGCAACTATGGTTACTCTGCATTATACTTGGATTGATTAGCATGCCTGTTGCAGTTGTCGTGAAATGCATACCCGTTGAAAGCAAAGTTAAACCCCCTGATGGCTATGAAGCCATCCCTGATGGTCCTGGCCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

855

Amino Acids

92.44

Weight (kDa)

5.04

Isoelectric Point (pI)

30.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 62 - 156 8.2e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 331 - 481 9.1e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 468 - 588 7e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 659 - 832 1.4e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 493
Acc36I ACCTGC 2 cut(s) 493, 958
AccB7I CCANNNNNTGG 2 cut(s) 281, 876
AccII CGCG 1 cut(s) 2402
AciI CCGC 1 cut(s) 1521
AclI AACGTT 1 cut(s) 1926
AclWI GGATC 1 cut(s) 1644
AcoI YGGCCR 1 cut(s) 50
AcsI RAATTY 7 cut(s) 78, 939, 1058, 1306, 1586, 1632, 1684
AcuI CTGAAG 2 cut(s) 723, 1341
AfaI GTAC 5 cut(s) 1491, 1846, 1883, 2098, 2418
AflIII ACRYGT 1 cut(s) 1596
AjnI CCWGG 4 cut(s) 1441, 2111, 2265, 2554
AleI CACNNNNGTG 1 cut(s) 1676
AloI GAACNNNNNNTCC 2 cut(s) 231, 263
Alw21I GWGCWC 2 cut(s) 952, 1161
Alw26I GTCTC 3 cut(s) 485, 791, 1605
AlwI GGATC 1 cut(s) 1644
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 5 cut(s) 50, 1644, 2078, 2114, 2557
ApeKI GCWGC 3 cut(s) 1475, 2191, 2194
ApoI RAATTY 7 cut(s) 78, 939, 1058, 1306, 1586, 1632, 1684
ArsI GACNNNNNNTTYG 4 cut(s) 1009, 1041, 1572, 1604
AseI ATTAAT 1 cut(s) 755
Asp700I GAANNNNTTC 1 cut(s) 1697
AspA2I CCTAGG 1 cut(s) 2344
AspLEI GCGC 2 cut(s) 1098, 2402
AspS9I GGNCC 8 cut(s) 441, 1429, 1580, 1645, 1731, 2079, 2115, 2552
AsuC2I CCSGG 1 cut(s) 1703
AsuNHI GCTAGC 1 cut(s) 2408
AvaII GGWCC 5 cut(s) 441, 1429, 1580, 1731, 2552
AvrII CCTAGG 1 cut(s) 2344
BalI TGGCCA 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 952, 1161
BbvI GCAGC 3 cut(s) 1462, 2181, 2203
BceAI ACGGC 1 cut(s) 2274
BcgI CGANNNNNNTGC 2 cut(s) 1070, 1104
BciT130I CCWGG 4 cut(s) 1443, 2113, 2267, 2556
BciVI GTATCC 1 cut(s) 293
BclI TGATCA 1 cut(s) 1941
BcnI CCSGG 1 cut(s) 1703
BcoDI GTCTC 3 cut(s) 485, 791, 1605
BfaI CTAG 8 cut(s) 192, 813, 1536, 1863, 2198, 2345, 2384, 2409
BfmI CTRYAG 4 cut(s) 500, 1605, 1848, 2046
BfuAI ACCTGC 2 cut(s) 493, 958
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 3 cut(s) 1476, 2192, 2195
BlnI CCTAGG 1 cut(s) 2344
BlsI GCNGC 3 cut(s) 1477, 2193, 2196
BmcAI AGTACT 1 cut(s) 1846
Bme1390I CCNGG 5 cut(s) 1443, 1703, 2113, 2267, 2556
Bme18I GGWCC 5 cut(s) 441, 1429, 1580, 1731, 2552
BmgT120I GGNCC 8 cut(s) 441, 1429, 1580, 1645, 1731, 2079, 2115, 2552
BmiI GGNNCC 5 cut(s) 388, 1125, 1431, 1732, 2081
BmrFI CCNGG 5 cut(s) 1443, 1703, 2113, 2267, 2556
BmsI GCATC 6 cut(s) 631, 790, 1078, 1214, 1963, 2206
BmtI GCTAGC 1 cut(s) 2412
BpiI GAAGAC 1 cut(s) 624
BpmI CTGGAG 1 cut(s) 966
BpuMI CCSGG 1 cut(s) 1703
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 6 cut(s) 12, 1442, 1674, 2182, 2317, 2344
BsaXI ACNNNNNCTCC 6 cut(s) 231, 261, 1245, 1275, 2038, 2068
Bse1I ACTGG 6 cut(s) 347, 957, 1906, 1912, 2046, 2212
Bse3DI GCAATG 3 cut(s) 619, 669, 750
BseBI CCWGG 4 cut(s) 1443, 2113, 2267, 2556
BseDI CCNNGG 6 cut(s) 12, 1442, 1674, 2182, 2317, 2344
BseGI GGATG 8 cut(s) 202, 957, 1012, 1168, 1383, 1567, 1837, 2541
BseMI GCAATG 3 cut(s) 619, 669, 750
BseMII CTCAG 5 cut(s) 461, 719, 801, 984, 2414
BseNI ACTGG 6 cut(s) 347, 957, 1906, 1912, 2046, 2212
BseRI GAGGAG 1 cut(s) 495
BseXI GCAGC 3 cut(s) 1462, 2181, 2203
BseYI CCCAGC 3 cut(s) 1092, 1478, 2334
Bsh1236I CGCG 1 cut(s) 2402
BshFI GGCC 5 cut(s) 52, 1646, 2080, 2116, 2559
BsiHKAI GWGCWC 2 cut(s) 952, 1161
BsiSI CCGG 1 cut(s) 1702
BslFI GGGAC 3 cut(s) 45, 1744, 1884
BsmAI GTCTC 3 cut(s) 485, 791, 1605
BsmFI GGGAC 3 cut(s) 45, 1744, 1884
BsmI GAATGC 1 cut(s) 2252
BsnI GGCC 5 cut(s) 52, 1646, 2080, 2116, 2559
Bso31I GGTCTC 1 cut(s) 485
Bsp1286I GDGCHC 2 cut(s) 952, 1161
Bsp1407I TGTACA 1 cut(s) 1881
Bsp143I GATC 4 cut(s) 618, 1264, 1649, 1941
BspACI CCGC 1 cut(s) 1521
BspANI GGCC 5 cut(s) 52, 1646, 2080, 2116, 2559
BspCNI CTCAG 5 cut(s) 462, 720, 800, 985, 2415
BspFNI CGCG 1 cut(s) 2402
BspLI GGNNCC 5 cut(s) 388, 1125, 1431, 1732, 2081
BspMAI CTGCAG 2 cut(s) 504, 1609
BspMI ACCTGC 2 cut(s) 493, 958
BspOI GCTAGC 1 cut(s) 2412
BspPI GGATC 1 cut(s) 1644
BspTNI GGTCTC 1 cut(s) 485
BsrDI GCAATG 3 cut(s) 619, 669, 750
BsrGI TGTACA 1 cut(s) 1881
BsrI ACTGG 6 cut(s) 347, 957, 1906, 1912, 2046, 2212
BssECI CCNNGG 6 cut(s) 12, 1442, 1674, 2182, 2317, 2344
BssMI GATC 4 cut(s) 618, 1264, 1649, 1941
BssT1I CCWWGG 2 cut(s) 1674, 2344
Bst2UI CCWGG 4 cut(s) 1443, 2113, 2267, 2556
Bst4CI ACNGT 8 cut(s) 430, 583, 1247, 1489, 1921, 2239, 2365, 2416
Bst6I CTCTTC 1 cut(s) 1372
BstAPI GCANNNNNTGC 1 cut(s) 139
BstAUI TGTACA 1 cut(s) 1881
BstC8I GCNNGC 4 cut(s) 1789, 2410, 2429, 2471
BstDEI CTNAG 6 cut(s) 470, 608, 728, 787, 993, 2423
BstDSI CCRYGG 3 cut(s) 12, 2182, 2317
BstEII GGTNACC 1 cut(s) 263
BstENI CCTNNNNNAGG 1 cut(s) 965
BstF5I GGATG 8 cut(s) 202, 957, 1012, 1168, 1383, 1567, 1837, 2541
BstFNI CGCG 1 cut(s) 2402
BstHHI GCGC 2 cut(s) 1098, 2402
BstKTI GATC 4 cut(s) 621, 1267, 1652, 1944
BstMAI GTCTC 3 cut(s) 485, 791, 1605
BstMBI GATC 4 cut(s) 618, 1264, 1649, 1941
BstMWI GCNNNNNNNGC 5 cut(s) 139, 508, 1322, 1788, 2367
BstNI CCWGG 4 cut(s) 1443, 2113, 2267, 2556
BstNSI RCATGY 4 cut(s) 64, 1600, 1962, 2473
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 5 cut(s) 1441, 1701, 2111, 2265, 2554
BstSFI CTRYAG 4 cut(s) 500, 1605, 1848, 2046
BstUI CGCG 1 cut(s) 2402
BstV1I GCAGC 3 cut(s) 1462, 2181, 2203
BstV2I GAAGAC 1 cut(s) 624
BstXI CCANNNNNNTGG 1 cut(s) 2427
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 5 cut(s) 52, 1646, 2080, 2116, 2559
BtgI CCRYGG 3 cut(s) 12, 2182, 2317
BtgZI GCGATG 1 cut(s) 1147
BtsCI GGATG 8 cut(s) 202, 957, 1012, 1168, 1383, 1567, 1837, 2541
BtsI GCAGTG 3 cut(s) 111, 264, 497
BveI ACCTGC 2 cut(s) 493, 958
Cac8I GCNNGC 4 cut(s) 1789, 2410, 2429, 2471
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 2 cut(s) 1098, 2402
Cfr13I GGNCC 8 cut(s) 441, 1429, 1580, 1645, 1731, 2079, 2115, 2552
CseI GACGC 2 cut(s) 1427, 1748
Csp6I GTAC 5 cut(s) 1490, 1845, 1882, 2097, 2417
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviQI GTAC 5 cut(s) 1490, 1845, 1882, 2097, 2417
DdeI CTNAG 6 cut(s) 470, 608, 728, 787, 993, 2423
DpnI GATC 4 cut(s) 620, 1266, 1651, 1943
DpnII GATC 4 cut(s) 618, 1264, 1649, 1941
DraI TTTAAA 1 cut(s) 1351
EaeI YGGCCR 1 cut(s) 50
Eam1104I CTCTTC 1 cut(s) 1372
EarI CTCTTC 1 cut(s) 1372
Eco130I CCWWGG 2 cut(s) 1674, 2344
Eco31I GGTCTC 1 cut(s) 485
Eco47I GGWCC 5 cut(s) 441, 1429, 1580, 1731, 2552
Eco57I CTGAAG 2 cut(s) 723, 1341
Eco91I GGTNACC 1 cut(s) 263
EcoNI CCTNNNNNAGG 1 cut(s) 965
EcoO109I RGGNCCY 2 cut(s) 1429, 2079
EcoO65I GGTNACC 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 1632
EcoRII CCWGG 4 cut(s) 1441, 2111, 2265, 2554
EcoT14I CCWWGG 2 cut(s) 1674, 2344
EcoT22I ATGCAT 2 cut(s) 2254, 2498
ErhI CCWWGG 2 cut(s) 1674, 2344
FalI AAGNNNNNCTT 2 cut(s) 1658, 1690
FaqI GGGAC 3 cut(s) 45, 1744, 1884
FauNDI CATATG 2 cut(s) 821, 858
FbaI TGATCA 1 cut(s) 1941
Fnu4HI GCNGC 3 cut(s) 1476, 2192, 2195
FokI GGATG 8 cut(s) 209, 944, 1019, 1155, 1370, 1574, 1844, 2528
Fsp4HI GCNGC 3 cut(s) 1476, 2192, 2195
FspBI CTAG 8 cut(s) 192, 813, 1536, 1863, 2198, 2345, 2384, 2409
GlaI GCGC 2 cut(s) 1097, 2401
GluI GCNGC 3 cut(s) 1476, 2192, 2195
GsaI CCCAGC 3 cut(s) 1096, 1482, 2338
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 5 cut(s) 52, 1646, 2080, 2116, 2559
HapII CCGG 1 cut(s) 1702
HgaI GACGC 2 cut(s) 1427, 1748
HhaI GCGC 2 cut(s) 1098, 2402
Hin6I GCGC 2 cut(s) 1096, 2400
HinP1I GCGC 2 cut(s) 1096, 2400
HincII GTYRAC 3 cut(s) 1917, 1924, 2008
HindII GTYRAC 3 cut(s) 1917, 1924, 2008
HindIII AAGCTT 2 cut(s) 325, 2101
HinfI GANTC 7 cut(s) 320, 730, 1043, 1256, 1364, 1422, 2202
HpaI GTTAAC 2 cut(s) 1917, 1924
HpaII CCGG 1 cut(s) 1702
Hpy166II GTNNAC 5 cut(s) 710, 1882, 1917, 1924, 2008
Hpy188I TCNGA 9 cut(s) 471, 937, 994, 1210, 1321, 1938, 2142, 2215, 2280
Hpy8I GTNNAC 5 cut(s) 710, 1882, 1917, 1924, 2008
HpyAV CCTTC 5 cut(s) 50, 698, 1444, 1571, 2057
HpyCH4III ACNGT 8 cut(s) 430, 583, 1247, 1489, 1921, 2239, 2365, 2416
HpyCH4IV ACGT 3 cut(s) 924, 1873, 1926
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 508, 1322, 1788, 2367
HpyF3I CTNAG 6 cut(s) 470, 608, 728, 787, 993, 2423
HpySE526I ACGT 3 cut(s) 924, 1873, 1926
HspAI GCGC 2 cut(s) 1096, 2400
Ksp22I TGATCA 1 cut(s) 1941
KspAI GTTAAC 2 cut(s) 1917, 1924
Kzo9I GATC 4 cut(s) 618, 1264, 1649, 1941
LmnI GCTCC 3 cut(s) 947, 1747, 1981
Lsp1109I GCAGC 3 cut(s) 1462, 2181, 2203
LweI GCATC 6 cut(s) 631, 790, 1078, 1214, 1963, 2206
MaeI CTAG 8 cut(s) 192, 813, 1536, 1863, 2198, 2345, 2384, 2409
MaeII ACGT 3 cut(s) 924, 1873, 1926
MalI GATC 4 cut(s) 620, 1266, 1651, 1943
MboI GATC 4 cut(s) 618, 1264, 1649, 1941
MfeI CAATTG 2 cut(s) 21, 2153
MhlI GDGCHC 2 cut(s) 952, 1161
MlsI TGGCCA 1 cut(s) 52
MluNI TGGCCA 1 cut(s) 52
MlyI GAGTC 5 cut(s) 329, 739, 1052, 1358, 2196
Mox20I TGGCCA 1 cut(s) 52
Mph1103I ATGCAT 2 cut(s) 2254, 2498
MroXI GAANNNNTTC 1 cut(s) 1697
MscI TGGCCA 1 cut(s) 52
MslI CAYNNNNRTG 3 cut(s) 1173, 1676, 2015
Msp20I TGGCCA 1 cut(s) 52
MspA1I CMGCKG 2 cut(s) 2194, 2334
MspI CCGG 1 cut(s) 1702
MspR9I CCNGG 5 cut(s) 1443, 1703, 2113, 2267, 2556
MunI CAATTG 2 cut(s) 21, 2153
Mva1269I GAATGC 1 cut(s) 2252
MvaI CCWGG 4 cut(s) 1443, 2113, 2267, 2556
MvnI CGCG 1 cut(s) 2402
MwoI GCNNNNNNNGC 5 cut(s) 139, 508, 1322, 1788, 2367
NciI CCSGG 1 cut(s) 1703
NdeI CATATG 2 cut(s) 821, 858
NdeII GATC 4 cut(s) 618, 1264, 1649, 1941
NheI GCTAGC 1 cut(s) 2408
NlaIV GGNNCC 5 cut(s) 388, 1125, 1431, 1732, 2081
NmuCI GTSAC 7 cut(s) 109, 187, 263, 562, 884, 1498, 1505
NsiI ATGCAT 2 cut(s) 2254, 2498
NspI RCATGY 4 cut(s) 64, 1600, 1962, 2473
OliI CACNNNNGTG 1 cut(s) 1676
PaeI GCATGC 1 cut(s) 2473
PaqCI CACCTGC 1 cut(s) 493
PciI ACATGT 1 cut(s) 1596
PctI GAATGC 1 cut(s) 2252
PdmI GAANNNNTTC 1 cut(s) 1697
PfeI GAWTC 2 cut(s) 1256, 1422
PflMI CCANNNNNTGG 2 cut(s) 281, 876
PfoI TCCNGGA 1 cut(s) 1701
PkrI GCNGC 3 cut(s) 1477, 2193, 2196
PleI GAGTC 5 cut(s) 328, 738, 1051, 1358, 2196
PpsI GAGTC 5 cut(s) 328, 738, 1051, 1358, 2196
PpuMI RGGWCCY 1 cut(s) 1429
PscI ACATGT 1 cut(s) 1596
PshBI ATTAAT 1 cut(s) 755
Psp1406I AACGTT 1 cut(s) 1926
Psp5II RGGWCCY 1 cut(s) 1429
Psp6I CCWGG 4 cut(s) 1441, 2111, 2265, 2554
PspEI GGTNACC 1 cut(s) 263
PspFI CCCAGC 3 cut(s) 1092, 1478, 2334
PspGI CCWGG 4 cut(s) 1441, 2111, 2265, 2554
PspN4I GGNNCC 5 cut(s) 388, 1125, 1431, 1732, 2081
PspPI GGNCC 8 cut(s) 441, 1429, 1580, 1645, 1731, 2079, 2115, 2552
PspPPI RGGWCCY 1 cut(s) 1429
PstI CTGCAG 2 cut(s) 504, 1609
PstNI CAGNNNCTG 1 cut(s) 701
PvuII CAGCTG 2 cut(s) 2194, 2334
RsaI GTAC 5 cut(s) 1491, 1846, 1883, 2098, 2418
RsaNI GTAC 5 cut(s) 1490, 1845, 1882, 2097, 2417
RseI CAYNNNNRTG 3 cut(s) 1173, 1676, 2015
SatI GCNGC 3 cut(s) 1476, 2192, 2195
Sau3AI GATC 4 cut(s) 618, 1264, 1649, 1941
Sau96I GGNCC 8 cut(s) 441, 1429, 1580, 1645, 1731, 2079, 2115, 2552
ScaI AGTACT 1 cut(s) 1846
SchI GAGTC 5 cut(s) 329, 739, 1052, 1358, 2196
ScrFI CCNGG 5 cut(s) 1443, 1703, 2113, 2267, 2556
SduI GDGCHC 2 cut(s) 952, 1161
SfaNI GCATC 6 cut(s) 631, 790, 1078, 1214, 1963, 2206
SfcI CTRYAG 4 cut(s) 500, 1605, 1848, 2046
SinI GGWCC 5 cut(s) 441, 1429, 1580, 1731, 2552
SmiMI CAYNNNNRTG 3 cut(s) 1173, 1676, 2015
SphI GCATGC 1 cut(s) 2473
SsiI CCGC 1 cut(s) 1521
SspMI CTAG 8 cut(s) 192, 813, 1536, 1863, 2198, 2345, 2384, 2409
StyD4I CCNGG 5 cut(s) 1441, 1701, 2111, 2265, 2554
StyI CCWWGG 2 cut(s) 1674, 2344
TaaI ACNGT 8 cut(s) 430, 583, 1247, 1489, 1921, 2239, 2365, 2416
TaiI ACGT 3 cut(s) 927, 1876, 1929
TaqI TCGA 1 cut(s) 2175
TaqII GACCGA 1 cut(s) 458
TatI WGTACW 2 cut(s) 1844, 1881
TfiI GAWTC 2 cut(s) 1256, 1422
TseFI GTSAC 7 cut(s) 109, 187, 263, 562, 884, 1498, 1505
TseI GCWGC 3 cut(s) 1475, 2191, 2194
Tsp45I GTSAC 7 cut(s) 109, 187, 263, 562, 884, 1498, 1505
TspGWI ACGGA 2 cut(s) 2171, 2306
Van91I CCANNNNNTGG 2 cut(s) 281, 876
VpaK11BI GGWCC 5 cut(s) 441, 1429, 1580, 1731, 2552
VspI ATTAAT 1 cut(s) 755
XagI CCTNNNNNAGG 1 cut(s) 965
XapI RAATTY 7 cut(s) 78, 939, 1058, 1306, 1586, 1632, 1684
XceI RCATGY 4 cut(s) 64, 1600, 1962, 2473
XcmI CCANNNNNNNNNTGG 1 cut(s) 856
XmaJI CCTAGG 1 cut(s) 2344
XmnI GAANNNNTTC 1 cut(s) 1697
XspI CTAG 8 cut(s) 192, 813, 1536, 1863, 2198, 2345, 2384, 2409
ZrmI AGTACT 1 cut(s) 1846
Zsp2I ATGCAT 2 cut(s) 2254, 2498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.