Rh3AG254300

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002172
Physical Location & Seq
Forward (+)
27697077 .. 27754484
57408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG254300.1

Sequence Viewer

Length: 2607 bp
ATGGAAAGCTACTTGCACAAGGACTTCAAAATCCAGCCCAAGCACCGCTCGCCGGAGGCTTCAAGAAACTGGAAGAAAGCCGTCGGCCTCATCGTCAAGAACCGGGCCAGAAGGTTCCGACATGTCCCCGATCCCGACAAGCGCGCCGAGGGTGAGAGGAAGAAGAAGGAAATCCAGGAAAAAATTCGAGTTGCTCTTTATGTTCAAAAAGCAGCACTAACATTCATTGAGGCTGGAGGTAGTCAACCAGTTACTGATCAGGTTGAATACAAACTGTCTGAAGAGGCCGAAAAAGAGGGTTTCAGCATTCACCCAGATGAACTTGCATCAATCATACGCAGCCATGATATTAAGGTCTTGAAAAGACACGGTGGAGTTTATGGGATTTTAATAAAAGTATCCGTAGTACTAGATGAAGGTGTCAGGAGTAGCAGTATACCAACAAGGCAAAATGTTTATGGCTTAAACCGCTATACGGAGAAACCTCCCCGATCATTCTTGGTGTTTGTATGGGAAGCTCTACAGGATTTAACTTTAATTATTCTTATGGTTTGTGCCGTGGTTTCAATTGGAGTGGGACTTGCTACTGACGGGTGGCCAGAAGGCATGTATGATGGGGTGGGAATTTTACTCAGTATAGCTTTAGTTGTTTTGGTCACTGCCATTAGTGATTACAGGCAGTCCATGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTTATTCAGGTCACTAGGGATGGGAAAAGACAAAAAGTTTCCATTTATGATTTGCTGGTGGGAGATATTGTTCATTTAGCAGTGGGTGACCTTGTTCCAGCAGATGGACTTTTCATATCAGGATACAGCTTGTTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGAATGTGTATGAAGAAAAGCCTTTTCTTCTTTCGGGAACCAAAGTGCAAGATGGGTCAGGGAAAATGCTGGTAACAACAGTTGGTATGAGGACCGAATGGGGAAAGTTGATGGAAACCCTGAGTGAGGGAGGAGAAGATGAGACCCCACTGCAGGTGAAGCTGAATGGTGTTGCTACAATTATTGGTAAAATTGGTTTGGCTTTCGCTGTGGTGACATTTTTGGTTTTGACAGTAAGATATTTGGTGGAAAAAGCACTTAGCAATGAGATCACAGATTGGTCTTCAACTGATGCTGTAACACTTTTGAACTACTTTGCCATTGCAGTAACTATAATTGTTGTTGCAGTTCCTGAAGGTTTACCATTGGCAGTAACACTGAGTCTTGCTTTTGCAATGAAGAAATTAATGAATGATAAGGCACTTGTGAGGCATCTCTCAGCCTGTGAGACAATGGGTTCTGCTAGTTGCATATGCACGGATAAAACAGGAACATTAACCACAAACCATATGGTAGTTACCAAGATATGGATAGGTGACAAATCTATAAATGTAAAAGGTAATGAAAGTAAAGACGTAGTAAAATCAGAAATTTCTGGAGCACTGGACATCCTATTGCAGGTTATATTTCAAAATACAAGCTCTGAGGTTATCAAGGATGAGGGAAAGACCTCCATTTTGGGAACACCAACAGAGTCAGCATTATTAGAATTTGGTTTGCTTTTGGGTGGCGATTTTGATGCCCTGCGCAGAGAGTTTAAGATTATTAAGATGGAACCTTTCAATTCTGTTAGGAAGAAGATGTCTGTGCTCATCGCTCATCCTGATGGTGGGATAAGAGCTTTTTGCAAAGGTGCATCTGAAATAGTATTGGGAATGTGCAACAAAGTTATTGACTGTAATGGAGAATCTGTTGATCTCTCCCAAAAAGAGGCAAATAATATCACAGATGTCATAAATTCTTTTGCTTCTGAAGCATTAAGAACTCTCTGCTTGGCTTTTAAAGATATACATGACTCTTCCATCAACAATGACATCCCCAATGATGGCTACACATTGATAGCAGTTGTAGGAATCAAGGACCCTGTGCGTCCTGGGGTGAAGGAAGCAGTCCAAACTTGTTTGGCTGCTGGGATAACTGTCCGAATGGTCACAGGTGACAATATAAATACTGCTAAAGCCATAGCTAGAGAATGTGGCATACTCACAGAGGATGGTTTGGCAATAGAAGGACCAGAATTTCGTAACATGTCTCCTGCAGATATGAAAGCTGTTATACCGAGGATTCAGGTTGGTAAAATTTTGTATTTCAGATCTTTGACCCTCAACTCAACCATATACGTTTTCAGTTTTATTATATGTTGTTGTATACAGGTGATGGCCCGATCCTTACCATTGGATAAGCACACCTTGGTGAAGAATTTGAGGAATACATTCCAGGAGGTAGTTGCAGTTACAGGTGATGGGACCAATGACGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGCACAGAGGTTTGTGTCTTAGCTACTTCTCTAGTTACAATCTCAAATCTTGAGTGCCTATCTATCACCTTGTTGCGCCCTTATGGGATCCTTTTTCTTTACCTTTTGTTTTTGTTTTTGTTCTTTTTTCTTTTCATTTTCTTTTTTAATTTCAACATCTGTTGCATAATGTTGTGCTTGCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

868

Amino Acids

95.31

Weight (kDa)

6.14

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 5 - 50 5.3e-12 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 123 - 189 3.3e-11 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 244 - 338 8.4e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 513 - 663 1.1e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 650 - 798 6e-12 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1039
Acc16I TGCGCA 1 cut(s) 1643
Acc36I ACCTGC 2 cut(s) 1039, 1504
AccB7I CCANNNNNTGG 2 cut(s) 827, 1422
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 2 cut(s) 436, 2263
AccII CGCG 1 cut(s) 144
AciI CCGC 2 cut(s) 46, 469
AclWI GGATC 4 cut(s) 125, 2274, 2500, 2513
AcoI YGGCCR 1 cut(s) 596
AcsI RAATTY 8 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2193, 2314
AcuI CTGAAG 3 cut(s) 300, 1269, 1887
AfaI GTAC 1 cut(s) 408
AflIII ACRYGT 2 cut(s) 121, 2142
AjnI CCWGG 3 cut(s) 174, 1987, 2331
AleI CACNNNNGTG 1 cut(s) 2306
AloI GAACNNNNNNTCC 2 cut(s) 777, 809
Alw21I GWGCWC 2 cut(s) 1498, 1707
Alw26I GTCTC 3 cut(s) 1031, 1337, 2151
AlwI GGATC 4 cut(s) 125, 2274, 2500, 2513
AlwNI CAGNNNCTG 2 cut(s) 254, 1247
AoxI GGCC 5 cut(s) 85, 105, 285, 596, 2274
ApeKI GCWGC 3 cut(s) 212, 339, 2021
ApoI RAATTY 8 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2193, 2314
ArsI GACNNNNNNTTYG 4 cut(s) 1555, 1587, 2118, 2150
AseI ATTAAT 1 cut(s) 1301
Asp700I GAANNNNTTC 2 cut(s) 183, 2327
AspLEI GCGC 4 cut(s) 144, 146, 1644, 2496
AspS9I GGNCC 6 cut(s) 105, 987, 1975, 2126, 2275, 2361
AsuC2I CCSGG 1 cut(s) 104
AvaII GGWCC 4 cut(s) 987, 1975, 2126, 2361
BalI TGGCCA 1 cut(s) 598
BamHI GGATCC 1 cut(s) 2505
BbsI GAAGAC 1 cut(s) 1170
Bbv12I GWGCWC 2 cut(s) 1498, 1707
BbvI GCAGC 3 cut(s) 224, 351, 2008
BceAI ACGGC 2 cut(s) 65, 542
BcgI CGANNNNNNTGC 2 cut(s) 1616, 1650
BciT130I CCWGG 3 cut(s) 176, 1989, 2333
BciVI GTATCC 2 cut(s) 409, 839
BclI TGATCA 1 cut(s) 256
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 3 cut(s) 1031, 1337, 2151
BfaI CTAG 5 cut(s) 410, 738, 1359, 2082, 2450
BfmI CTRYAG 3 cut(s) 521, 1046, 2151
BfuAI ACCTGC 2 cut(s) 1039, 1504
BfuI GTATCC 2 cut(s) 409, 839
BglII AGATCT 1 cut(s) 2207
BisI GCNGC 3 cut(s) 213, 340, 2022
BlsI GCNGC 3 cut(s) 214, 341, 2023
BmcAI AGTACT 1 cut(s) 408
Bme1390I CCNGG 4 cut(s) 104, 176, 1989, 2333
Bme18I GGWCC 4 cut(s) 987, 1975, 2126, 2361
BmgT120I GGNCC 6 cut(s) 105, 987, 1975, 2126, 2275, 2361
BmiI GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2362, 2507
BmrFI CCNGG 4 cut(s) 104, 176, 1989, 2333
BmsI GCATC 5 cut(s) 335, 1177, 1336, 1624, 1760
BpiI GAAGAC 1 cut(s) 1170
BpmI CTGGAG 2 cut(s) 255, 1512
BpuEI CTTGAG 1 cut(s) 2489
BpuMI CCSGG 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 5 cut(s) 147, 558, 1988, 2174, 2304
BsaXI ACNNNNNCTCC 6 cut(s) 418, 448, 777, 807, 1791, 1821
Bse1I ACTGG 4 cut(s) 74, 248, 893, 1503
Bse3DI GCAATG 3 cut(s) 1165, 1215, 1296
BseBI CCWGG 3 cut(s) 176, 1989, 2333
BseDI CCNNGG 5 cut(s) 147, 558, 1988, 2174, 2304
BseGI GGATG 6 cut(s) 748, 1503, 1558, 1714, 1929, 2113
BseMI GCAATG 3 cut(s) 1165, 1215, 1296
BseMII CTCAG 5 cut(s) 646, 1007, 1265, 1347, 1530
BseNI ACTGG 4 cut(s) 74, 248, 893, 1503
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 1041
BseXI GCAGC 3 cut(s) 224, 351, 2008
BseYI CCCAGC 1 cut(s) 2024
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 5 cut(s) 87, 107, 287, 598, 2276
BsiHKAI GWGCWC 2 cut(s) 1498, 1707
BsiSI CCGG 2 cut(s) 53, 103
BslFI GGGAC 3 cut(s) 110, 591, 2374
BsmAI GTCTC 3 cut(s) 1031, 1337, 2151
BsmFI GGGAC 3 cut(s) 110, 591, 2374
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 5 cut(s) 87, 107, 287, 598, 2276
Bso31I GGTCTC 1 cut(s) 1031
Bsp1286I GDGCHC 2 cut(s) 1498, 1707
Bsp143I GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
BspACI CCGC 2 cut(s) 46, 469
BspANI GGCC 5 cut(s) 87, 107, 287, 598, 2276
BspCNI CTCAG 5 cut(s) 645, 1008, 1266, 1346, 1531
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2362, 2507
BspMAI CTGCAG 2 cut(s) 1050, 2155
BspMI ACCTGC 2 cut(s) 1039, 1504
BspPI GGATC 4 cut(s) 125, 2274, 2500, 2513
BspTNI GGTCTC 1 cut(s) 1031
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 3 cut(s) 1165, 1215, 1296
BsrI ACTGG 4 cut(s) 74, 248, 893, 1503
BssECI CCNNGG 5 cut(s) 147, 558, 1988, 2174, 2304
BssHII GCGCGC 1 cut(s) 142
BssMI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
BssNAI GTATAC 2 cut(s) 437, 2264
BssT1I CCWWGG 1 cut(s) 2304
Bst1107I GTATAC 2 cut(s) 437, 2264
Bst2UI CCWGG 3 cut(s) 176, 1989, 2333
Bst4CI ACNGT 6 cut(s) 276, 371, 976, 1129, 1793, 2035
Bst6I CTCTTC 2 cut(s) 276, 1918
BstAPI GCANNNNNTGC 1 cut(s) 685
BstC8I GCNNGC 4 cut(s) 50, 144, 2419, 2597
BstDEI CTNAG 7 cut(s) 632, 1016, 1154, 1274, 1333, 1539, 2437
BstDSI CCRYGG 1 cut(s) 558
BstEII GGTNACC 1 cut(s) 809
BstENI CCTNNNNNAGG 2 cut(s) 1019, 1511
BstF5I GGATG 6 cut(s) 748, 1503, 1558, 1714, 1929, 2113
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 4 cut(s) 144, 146, 1644, 2496
BstKTI GATC 8 cut(s) 133, 259, 494, 1167, 1813, 2210, 2282, 2508
BstMAI GTCTC 3 cut(s) 1031, 1337, 2151
BstMBI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
BstMWI GCNNNNNNNGC 6 cut(s) 49, 468, 685, 1054, 1868, 2418
BstNI CCWGG 3 cut(s) 176, 1989, 2333
BstNSI RCATGY 3 cut(s) 125, 610, 2146
BstPI GGTNACC 1 cut(s) 809
BstSCI CCNGG 4 cut(s) 102, 174, 1987, 2331
BstSFI CTRYAG 3 cut(s) 521, 1046, 2151
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 224, 351, 2008
BstV2I GAAGAC 1 cut(s) 1170
BstX2I RGATCY 2 cut(s) 2207, 2505
BstYI RGATCY 2 cut(s) 2207, 2505
BstZ17I GTATAC 2 cut(s) 437, 2264
BsuI GTATCC 2 cut(s) 409, 839
BsuRI GGCC 5 cut(s) 87, 107, 287, 598, 2276
BtgI CCRYGG 1 cut(s) 558
BtgZI GCGATG 1 cut(s) 1693
BtsCI GGATG 6 cut(s) 748, 1503, 1558, 1714, 1929, 2113
BtsI GCAGTG 3 cut(s) 657, 810, 1043
BtsIMutI CAGTG 6 cut(s) 657, 810, 900, 1043, 1271, 1496
BveI ACCTGC 2 cut(s) 1039, 1504
Cac8I GCNNGC 4 cut(s) 50, 144, 2419, 2597
CaiI CAGNNNCTG 2 cut(s) 254, 1247
CfoI GCGC 4 cut(s) 144, 146, 1644, 2496
Cfr13I GGNCC 6 cut(s) 105, 987, 1975, 2126, 2275, 2361
CseI GACGC 2 cut(s) 1973, 2378
Csp6I GTAC 1 cut(s) 407
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviAII CATG 7 cut(s) 122, 344, 607, 685, 1907, 2143, 2384
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 7 cut(s) 632, 1016, 1154, 1274, 1333, 1539, 2437
DpnI GATC 8 cut(s) 132, 258, 493, 1166, 1812, 2209, 2281, 2507
DpnII GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
DraI TTTAAA 1 cut(s) 1897
EaeI YGGCCR 1 cut(s) 596
Eam1104I CTCTTC 2 cut(s) 276, 1918
EarI CTCTTC 2 cut(s) 276, 1918
Eco130I CCWWGG 1 cut(s) 2304
Eco31I GGTCTC 1 cut(s) 1031
Eco47I GGWCC 4 cut(s) 987, 1975, 2126, 2361
Eco57I CTGAAG 3 cut(s) 300, 1269, 1887
Eco91I GGTNACC 1 cut(s) 809
EcoNI CCTNNNNNAGG 2 cut(s) 1019, 1511
EcoO109I RGGNCCY 1 cut(s) 1975
EcoO65I GGTNACC 1 cut(s) 809
EcoRII CCWGG 3 cut(s) 174, 1987, 2331
EcoT14I CCWWGG 1 cut(s) 2304
ErhI CCWWGG 1 cut(s) 2304
FaeI CATG 7 cut(s) 125, 347, 610, 688, 1910, 2146, 2387
FalI AAGNNNNNCTT 2 cut(s) 2288, 2320
FaqI GGGAC 3 cut(s) 110, 591, 2374
FatI CATG 7 cut(s) 121, 343, 606, 684, 1906, 2142, 2383
FauNDI CATATG 2 cut(s) 1367, 1404
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 2 cut(s) 436, 2263
Fnu4HI GCNGC 3 cut(s) 213, 340, 2022
FokI GGATG 6 cut(s) 755, 1490, 1565, 1701, 1916, 2120
Fsp4HI GCNGC 3 cut(s) 213, 340, 2022
FspBI CTAG 5 cut(s) 410, 738, 1359, 2082, 2450
FspI TGCGCA 1 cut(s) 1643
GlaI GCGC 4 cut(s) 143, 145, 1643, 2495
GluI GCNGC 3 cut(s) 213, 340, 2022
GsaI CCCAGC 1 cut(s) 2028
GsuI CTGGAG 2 cut(s) 255, 1512
HaeIII GGCC 5 cut(s) 87, 107, 287, 598, 2276
HapII CCGG 2 cut(s) 53, 103
HgaI GACGC 2 cut(s) 1973, 2378
HhaI GCGC 4 cut(s) 144, 146, 1644, 2496
Hin1II CATG 7 cut(s) 125, 347, 610, 688, 1910, 2146, 2387
Hin6I GCGC 4 cut(s) 142, 144, 1642, 2494
HinP1I GCGC 4 cut(s) 142, 144, 1642, 2494
HincII GTYRAC 1 cut(s) 245
HindII GTYRAC 1 cut(s) 245
HindIII AAGCTT 1 cut(s) 871
HinfI GANTC 7 cut(s) 866, 1276, 1589, 1802, 1910, 1968, 2179
HpaII CCGG 2 cut(s) 53, 103
Hpy166II GTNNAC 4 cut(s) 245, 437, 1256, 2264
Hpy188I TCNGA 8 cut(s) 119, 280, 1483, 1540, 1756, 1867, 2039, 2207
Hpy8I GTNNAC 4 cut(s) 245, 437, 1256, 2264
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 7 cut(s) 105, 160, 410, 596, 1244, 1990, 2117
HpyCH4III ACNGT 6 cut(s) 276, 371, 976, 1129, 1793, 2035
HpyCH4IV ACGT 2 cut(s) 1470, 2235
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 468, 685, 1054, 1868, 2418
HpyF3I CTNAG 7 cut(s) 632, 1016, 1154, 1274, 1333, 1539, 2437
HpySE526I ACGT 2 cut(s) 1470, 2235
Hsp92II CATG 7 cut(s) 125, 347, 610, 688, 1910, 2146, 2387
HspAI GCGC 4 cut(s) 142, 144, 1642, 2494
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
LmnI GCTCC 2 cut(s) 1493, 2377
Lsp1109I GCAGC 3 cut(s) 224, 351, 2008
LweI GCATC 5 cut(s) 335, 1177, 1336, 1624, 1760
MaeI CTAG 5 cut(s) 410, 738, 1359, 2082, 2450
MaeII ACGT 2 cut(s) 1470, 2235
MalI GATC 8 cut(s) 132, 258, 493, 1166, 1812, 2209, 2281, 2507
MbiI CCGCTC 1 cut(s) 48
MboI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 2 cut(s) 2207, 2505
MhlI GDGCHC 2 cut(s) 1498, 1707
MlsI TGGCCA 1 cut(s) 598
MluNI TGGCCA 1 cut(s) 598
MlyI GAGTC 4 cut(s) 875, 1285, 1598, 1904
MmeI TCCRAC 1 cut(s) 142
Mox20I TGGCCA 1 cut(s) 598
MroXI GAANNNNTTC 2 cut(s) 183, 2327
MscI TGGCCA 1 cut(s) 598
MslI CAYNNNNRTG 3 cut(s) 315, 1719, 2306
Msp20I TGGCCA 1 cut(s) 598
MspI CCGG 2 cut(s) 53, 103
MspR9I CCNGG 4 cut(s) 104, 176, 1989, 2333
MunI CAATTG 1 cut(s) 567
Mva1269I GAATGC 1 cut(s) 306
MvaI CCWGG 3 cut(s) 176, 1989, 2333
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 6 cut(s) 49, 468, 685, 1054, 1868, 2418
NciI CCSGG 1 cut(s) 104
NdeI CATATG 2 cut(s) 1367, 1404
NdeII GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
NlaIII CATG 7 cut(s) 125, 347, 610, 688, 1910, 2146, 2387
NlaIV GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2362, 2507
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
NsbI TGCGCA 1 cut(s) 1643
NspI RCATGY 3 cut(s) 125, 610, 2146
OliI CACNNNNGTG 1 cut(s) 2306
PaqCI CACCTGC 1 cut(s) 1039
PauI GCGCGC 1 cut(s) 142
PciI ACATGT 2 cut(s) 121, 2142
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 306
PdmI GAANNNNTTC 2 cut(s) 183, 2327
PfeI GAWTC 3 cut(s) 1802, 1968, 2179
PflMI CCANNNNNTGG 2 cut(s) 827, 1422
PfoI TCCNGGA 2 cut(s) 174, 2331
PkrI GCNGC 3 cut(s) 214, 341, 2023
PleI GAGTC 4 cut(s) 874, 1284, 1597, 1904
PpsI GAGTC 4 cut(s) 874, 1284, 1597, 1904
PpuMI RGGWCCY 1 cut(s) 1975
PscI ACATGT 2 cut(s) 121, 2142
PshBI ATTAAT 1 cut(s) 1301
Psp5II RGGWCCY 1 cut(s) 1975
Psp6I CCWGG 3 cut(s) 174, 1987, 2331
PspEI GGTNACC 1 cut(s) 809
PspFI CCCAGC 1 cut(s) 2024
PspGI CCWGG 3 cut(s) 174, 1987, 2331
PspN4I GGNNCC 6 cut(s) 116, 934, 1671, 1977, 2362, 2507
PspPI GGNCC 6 cut(s) 105, 987, 1975, 2126, 2275, 2361
PspPPI RGGWCCY 1 cut(s) 1975
PstI CTGCAG 2 cut(s) 1050, 2155
PstNI CAGNNNCTG 2 cut(s) 254, 1247
PsuI RGATCY 2 cut(s) 2207, 2505
PteI GCGCGC 1 cut(s) 142
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
RseI CAYNNNNRTG 3 cut(s) 315, 1719, 2306
SatI GCNGC 3 cut(s) 213, 340, 2022
Sau3AI GATC 8 cut(s) 130, 256, 491, 1164, 1810, 2207, 2279, 2505
Sau96I GGNCC 6 cut(s) 105, 987, 1975, 2126, 2275, 2361
ScaI AGTACT 1 cut(s) 408
SchI GAGTC 4 cut(s) 875, 1285, 1598, 1904
ScrFI CCNGG 4 cut(s) 104, 176, 1989, 2333
SduI GDGCHC 2 cut(s) 1498, 1707
SfaNI GCATC 5 cut(s) 335, 1177, 1336, 1624, 1760
SfcI CTRYAG 3 cut(s) 521, 1046, 2151
SinI GGWCC 4 cut(s) 987, 1975, 2126, 2361
SmiMI CAYNNNNRTG 3 cut(s) 315, 1719, 2306
SmlI CTYRAG 1 cut(s) 2468
SmoI CTYRAG 1 cut(s) 2468
SsiI CCGC 2 cut(s) 46, 469
SspMI CTAG 5 cut(s) 410, 738, 1359, 2082, 2450
StyD4I CCNGG 4 cut(s) 102, 174, 1987, 2331
StyI CCWWGG 1 cut(s) 2304
TaaI ACNGT 6 cut(s) 276, 371, 976, 1129, 1793, 2035
TaiI ACGT 2 cut(s) 1473, 2238
TaqI TCGA 1 cut(s) 187
TaqII GACCGA 1 cut(s) 1004
TatI WGTACW 1 cut(s) 406
TfiI GAWTC 3 cut(s) 1802, 1968, 2179
TscAI CASTG 6 cut(s) 664, 810, 900, 1050, 1278, 1503
TseFI GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
TseI GCWGC 3 cut(s) 212, 339, 2021
Tsp45I GTSAC 7 cut(s) 655, 733, 809, 1108, 1430, 2044, 2051
TspGWI ACGGA 3 cut(s) 391, 491, 1388
TspRI CASTG 6 cut(s) 664, 810, 900, 1050, 1278, 1503
Van91I CCANNNNNTGG 2 cut(s) 827, 1422
VpaK11BI GGWCC 4 cut(s) 987, 1975, 2126, 2361
VspI ATTAAT 1 cut(s) 1301
XagI CCTNNNNNAGG 2 cut(s) 1019, 1511
XapI RAATTY 8 cut(s) 183, 624, 1485, 1604, 1852, 2132, 2193, 2314
XceI RCATGY 3 cut(s) 125, 610, 2146
XcmI CCANNNNNNNNNTGG 1 cut(s) 1402
XmiI GTMKAC 2 cut(s) 436, 2263
XmnI GAANNNNTTC 2 cut(s) 183, 2327
XspI CTAG 5 cut(s) 410, 738, 1359, 2082, 2450
ZrmI AGTACT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.