Prupe.7G054400_v2.0.a1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0002172
Physical Location & Seq
Reverse (-)
9303583 .. 9311374
7792 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G054400.8

Sequence Viewer

Length: 2892 bp
ATGCATGGTGCTGGTGATCGATCCAATGAAAAGCCAGGACAGGATGAATACAAGCTGTCTGAAGATGCCAGAATTGCAGGTTTCAGCATTTACCCAGATGAACTTGCATCTATCACGTGTGGCCATGATATTCAGGCCTTGAAAATGCATGGTGGAATTCATGGGATTTTAAGGAAAGTCTCTGTCTCGCTAGATGAAGGTGTCAAGGATAGTAACATACCAATCAGGCAAAATGTTTATGGCTTAAACCGCTATACGGAGAAACCTCCTGGAACATTTTTTATGTTTGTATGGGAAGCCCTACAAGACTTAACATTAATTATCCTTATGGTATGTGCTGTGGTTTCAATAGGAGTTGGAATTGCCACTGAGGGGTGGCCAAAAGGCATGGACGATGGTGTGGGAATTTTAATTAGTATAGTCTTAGTCGTTGTGGTTACTGCCATTAGTGACTACAGACAAGCATTGCAATTCAAGGATTTGGACAGGGAGAAGAAAAAGATTTTCGTTCAGGTCACTAGGGATAAAAAAAGACAAAAAGTTTCCATTTATGACTTGGTTGTTGGAGATATTGTCCATTTGTCAATTGGGGACCAAGTTCCAGCAGATGGATTTTTCATATCAGGATACTCTTTGCTGATTGATGAGTCAAGCTTGTCAGGTGAGAGTGAGCCAGTGAATGTATATGAAGAGAAGCCTTTTCTTCTTTCAGGAACTAAAGTGCAGGATGGGTCAGGTATAATGCTGGTGACAACAGTTGGTATGAGAACTAAATGGGGAAAGTTGATGACAGCTCTGAGTGAAGAAGAAGAAGATGAGACCCCACTGCAGGTAAAGCTGAATGGTGTTGCTATAATTATTGGTAAAATTGGTCTGACTTTTGCTGTGTTGACATTTTCGGTATTGGCAGTAAGATTTTTGGTGGAAAAAATACTTAACAACGAGATCACTGATTGGTCTTCAACCGATGCGGTAATCCTATTGAACTACTTTGCTATTGCGGTAACTATAATTGTTGTTGCAGTTCCCGAAGGATTACTATTGGCAGCGACGCTGAGCCTTGCTTTTGCTATGAAAAAATTAATGAATGATAGGGCACTCGTAAGGCATCTCTCAGCATGTGAGACAATGGGTTCTGCTAGTTGCATTTGCACAGATAAAACAGGGACATTAACCACAAACCACATGGTAGTTAACAAGATATGGATATGTGGAAAACCTTTAGATGTAAAAGGTAATGAGAGTAAAGAAATATTGAGTTCAGAAATATCTGGAGCATCAAGCATCCTTTTACAGGTTATATTCCAAAATACAAGCTCTGAGGTTGTTATGGAAGATGGAAAGACCTCCATTTTGGGGACACCAACAGAGTTAGCATTATTAGAATTTGGTTTACTTTTGGGTGGCGATTTTGATGCCGTGCGCAGAGAGGTTAATATTCATAAGGTTGAACCTTTCAATTTTGTGAGGAAGAAAATGTCTGTGCTTGTAGATCATCCTCATGGTGGGAAACGAGCCTTTTGCAAAGGTGCATCAGAAATAGTACTGAGAATGTGTAACAAATTTATTGACTTTAATGGAGAATCTGTTATTCTCTCCCTAGAACAGGTAAAGAATATCACGGATGTCATTAATTCTTTTGCTTCTGAAGCCTTGAGAACGCTCTGCTTGGCTTTCAAAAATATAGATGACTCTTCCATCGAAAATGAAATCCCAGATGATGGCTATACATTGATAGCAGTTGTCGGTATCAAGGATCCTGTGCGCCCTGGGGTCAAGGATGCAGTTCAAACTTGTTTAGCTGCTGGAATAACTGTACGAATGGTCACTGGTGACAATTTAAATACGGCTAAAGCCATAGCTAAAGAATGCGGCATACTCACAGAAGATGGTTTAGCCATAGAAGGACAGGAGTTTCATAACATGTCTCCTGAGCAGAAGAAAGCTGTTATACCGAGAATTCAGGTGATGGCCCAGTCTTTACCATTGGATAAGCACACCTTGGTAAAAACTCTGAGAGATGAATTTGGTGAGGTAGTTGCAGTGACTGGCGATGGGACCAACGACGCTCCTGCTTTGCATGAGGCAGACATTGGACTTGCTATGGGCATAGCAGGAACAGAGGTTGCCAAGGAAAGTGCTGATGTTATCATATTGGATGACAATTTTAAGACTATAGTAAATGTGGCCAGATGGGGACGTTCAGTGTACGTAAACATTCAAAAGTTTGTGCAGTTCCAGTTGACAGTTAACGTTGTTGCTCTAATAATCAATTTTGTTTCTGCATGTGTCTCGGGATCTGCTCCCCTTACGGCTGTGCAATTGCTTTGGGTCAACATGATTATGGACCCTCTTGGGGCATTGGCACTGGCAACAGAGCCTCCAAATGATGGACTTATGAAAAGGCCCCCTGTTGGGAGGGATTCAAGCTTCATAACCAAGGCCATGTGGAGGAATATCATTGGCCAGAGTATCTATCAACTGATTGTCCTTGGAGTTCTCAATTTCTATGGGAAACAGCTACTTGGACTTTCTGGTTCAGATGCAACTGAAGTTCTAGACACTGTGATATTCAACGCATTTGTGTTTTGCCAGGTGTTTAATGAGATAAACAGCCGTGACATAGAGAAGAAAAACATATTCGTGGGCATGTTTGACAGCCGGGTATTCCTAGGCGTCGTGGTTTGCACAGTGGCCTTCCAAGTTATCATTGTAGAGTTCTTGGGAGCTTTTGCTAGCACTGTTCCACTAAGCTGGCAACTATGGTTACTCTGCATTTTACTTGGATCAGTTAGCATGCTTGTTGCAGTTGTGCTCAAGAACCCTTGCAGGAGGTTCCGCTTCGTCGCCAATCTCGATAAGGGGTCCGAGGCCGACAAGAAGCGGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009624 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

964

Amino Acids

104.96

Weight (kDa)

5.32

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1424
Acc36I ACCTGC 2 cut(s) 68, 820
AccB7I CCANNNNNTGG 3 cut(s) 608, 1721, 2390
AciI CCGC 6 cut(s) 250, 971, 1001, 1872, 2839, 2884
AclI AACGTT 1 cut(s) 2253
AclWI GGATC 5 cut(s) 15, 1751, 1764, 2305, 2794
AcoI YGGCCR 4 cut(s) 121, 377, 2187, 2464
AcsI RAATTY 6 cut(s) 156, 405, 1385, 1562, 1959, 2024
AcuI CTGAAG 3 cut(s) 81, 1668, 2571
AcvI CACGTG 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 2676
AfaI GTAC 3 cut(s) 1545, 1818, 2210
AflIII ACRYGT 2 cut(s) 116, 1923
AjnI CCWGG 4 cut(s) 34, 268, 1768, 2592
Alw21I GWGCWC 1 cut(s) 2817
Alw26I GTCTC 6 cut(s) 184, 190, 812, 1118, 1932, 2296
AlwI GGATC 5 cut(s) 15, 1751, 1764, 2305, 2794
AlwNI CAGNNNCTG 1 cut(s) 2048
Ama87I CYCGRG 1 cut(s) 2293
ApeKI GCWGC 2 cut(s) 1046, 1802
ApoI RAATTY 6 cut(s) 156, 405, 1385, 1562, 1959, 2024
ArsI GACNNNNNNTTYG 2 cut(s) 1336, 1368
AseI ATTAAT 3 cut(s) 317, 1082, 1632
AspA2I CCTAGG 1 cut(s) 2671
AspLEI GCGC 2 cut(s) 1425, 1767
AspS9I GGNCC 6 cut(s) 592, 1972, 2058, 2347, 2406, 2865
AsuC2I CCSGG 1 cut(s) 2663
AsuHPI GGTGA 6 cut(s) 26, 674, 760, 1844, 1978, 2042
AsuNHI GCTAGC 1 cut(s) 2735
AvaI CYCGRG 1 cut(s) 2293
AvaII GGWCC 4 cut(s) 592, 2058, 2347, 2865
AvrII CCTAGG 1 cut(s) 2671
BaeGI GKGCMC 1 cut(s) 1099
BalI TGGCCA 4 cut(s) 123, 379, 2189, 2466
BamHI GGATCC 1 cut(s) 1756
BbrPI CACGTG 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 951
Bbv12I GWGCWC 1 cut(s) 2817
BbvI GCAGC 2 cut(s) 1058, 1789
BceAI ACGGC 4 cut(s) 1403, 1863, 2328, 2601
BcgI CGANNNNNNTGC 8 cut(s) 1397, 1431, 1503, 1537, 2054, 2088, 2274, 2308
BciT130I CCWGG 4 cut(s) 36, 270, 1770, 2594
BciVI GTATCC 1 cut(s) 620
BcnI CCSGG 1 cut(s) 2663
BcoDI GTCTC 6 cut(s) 184, 190, 812, 1118, 1932, 2296
BfaI CTAG 7 cut(s) 191, 519, 1140, 1601, 2558, 2672, 2736
BfmI CTRYAG 3 cut(s) 454, 827, 2175
BfuAI ACCTGC 2 cut(s) 68, 820
BfuI GTATCC 1 cut(s) 620
BisI GCNGC 3 cut(s) 1047, 1803, 1873
BlnI CCTAGG 1 cut(s) 2671
BlpI GCTNAGC 1 cut(s) 1055
BlsI GCNGC 3 cut(s) 1048, 1804, 1874
BmcAI AGTACT 1 cut(s) 1545
Bme1390I CCNGG 5 cut(s) 36, 270, 1770, 2594, 2663
Bme18I GGWCC 4 cut(s) 592, 2058, 2347, 2865
BmeT110I CYCGRG 1 cut(s) 2293
BmgT120I GGNCC 6 cut(s) 592, 1972, 2058, 2347, 2406, 2865
BmiI GGNNCC 7 cut(s) 593, 1758, 2059, 2349, 2408, 2837, 2866
BmrFI CCNGG 5 cut(s) 36, 270, 1770, 2594, 2663
BmrI ACTGGG 1 cut(s) 1969
BmtI GCTAGC 1 cut(s) 2739
BmuI ACTGGG 1 cut(s) 1969
BpiI GAAGAC 1 cut(s) 951
BpmI CTGGAG 1 cut(s) 1293
Bpu10I CCTNAGC 1 cut(s) 1932
Bpu1102I GCTNAGC 1 cut(s) 1055
BpuEI CTTGAG 2 cut(s) 1675, 2801
BpuMI CCSGG 1 cut(s) 2663
Bsa29I ATCGAT 1 cut(s) 19
BsaAI YACGTR 2 cut(s) 117, 2212
BsaHI GRCGYC 1 cut(s) 2676
BsaI GGTCTC 1 cut(s) 812
BsaJI CCNNGG 8 cut(s) 1768, 1769, 2001, 2130, 2439, 2491, 2671, 2868
BsaXI ACNNNNNCTCC 6 cut(s) 558, 588, 1572, 1602, 2365, 2395
Bse1I ACTGG 6 cut(s) 674, 1834, 1975, 2053, 2239, 2373
Bse3DI GCAATG 1 cut(s) 464
BseBI CCWGG 4 cut(s) 36, 270, 1770, 2594
BseCI ATCGAT 1 cut(s) 19
BseDI CCNNGG 8 cut(s) 1768, 1769, 2001, 2130, 2439, 2491, 2671, 2868
BseGI GGATG 7 cut(s) 49, 733, 1284, 1495, 1630, 1786, 2164
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 8 cut(s) 360, 788, 1046, 1128, 1311, 1538, 1923, 2006
BseNI ACTGG 6 cut(s) 674, 1834, 1975, 2053, 2239, 2373
BseSI GKGCMC 1 cut(s) 1099
BseXI GCAGC 2 cut(s) 1058, 1789
BsgI GTGCAG 2 cut(s) 743, 2252
BshVI ATCGAT 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 2817
BsiHKCI CYCGRG 1 cut(s) 2293
BsiSI CCGG 1 cut(s) 2662
BslFI GGGAC 5 cut(s) 605, 1180, 1372, 2071, 2211
BsmAI GTCTC 6 cut(s) 184, 190, 812, 1118, 1932, 2296
BsmFI GGGAC 5 cut(s) 605, 1180, 1372, 2071, 2211
BsmI GAATGC 1 cut(s) 1874
Bso31I GGTCTC 1 cut(s) 812
BsoBI CYCGRG 1 cut(s) 2293
Bsp1286I GDGCHC 2 cut(s) 1099, 2817
Bsp143I GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
Bsp1720I GCTNAGC 1 cut(s) 1055
BspACI CCGC 6 cut(s) 250, 971, 1001, 1872, 2839, 2884
BspCNI CTCAG 8 cut(s) 361, 789, 1047, 1127, 1312, 1539, 1924, 2007
BspDI ATCGAT 1 cut(s) 19
BspLI GGNNCC 7 cut(s) 593, 1758, 2059, 2349, 2408, 2837, 2866
BspMAI CTGCAG 1 cut(s) 831
BspMI ACCTGC 2 cut(s) 68, 820
BspOI GCTAGC 1 cut(s) 2739
BspPI GGATC 5 cut(s) 15, 1751, 1764, 2305, 2794
BspTNI GGTCTC 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 464
BsrI ACTGG 6 cut(s) 674, 1834, 1975, 2053, 2239, 2373
BssECI CCNNGG 8 cut(s) 1768, 1769, 2001, 2130, 2439, 2491, 2671, 2868
BssMI GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
BssNI GRCGYC 1 cut(s) 2676
BssT1I CCWWGG 5 cut(s) 2001, 2130, 2439, 2491, 2671
Bst2UI CCWGG 4 cut(s) 36, 270, 1770, 2594
Bst4CI ACNGT 6 cut(s) 757, 1816, 2248, 2566, 2692, 2743
Bst6I CTCTTC 2 cut(s) 684, 1699
BstACI GRCGYC 1 cut(s) 2676
BstBAI YACGTR 2 cut(s) 117, 2212
BstC8I GCNNGC 3 cut(s) 2737, 2756, 2798
BstENI CCTNNNNNAGG 2 cut(s) 1292, 1604
BstF5I GGATG 7 cut(s) 49, 733, 1284, 1495, 1630, 1786, 2164
BstHHI GCGC 2 cut(s) 1425, 1767
BstKTI GATC 7 cut(s) 19, 23, 948, 1495, 1759, 2300, 2789
BstMAI GTCTC 6 cut(s) 184, 190, 812, 1118, 1932, 2296
BstMBI GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
BstMWI GCNNNNNNNGC 4 cut(s) 74, 249, 835, 1649
BstNI CCWGG 4 cut(s) 36, 270, 1770, 2594
BstNSI RCATGY 5 cut(s) 1122, 1927, 2289, 2653, 2800
BstSCI CCNGG 5 cut(s) 34, 268, 1768, 2592, 2661
BstSFI CTRYAG 3 cut(s) 454, 827, 2175
BstSLI GKGCMC 1 cut(s) 1099
BstSNI TACGTA 1 cut(s) 2212
BstV1I GCAGC 2 cut(s) 1058, 1789
BstV2I GAAGAC 1 cut(s) 951
BstX2I RGATCY 2 cut(s) 1756, 2297
BstXI CCANNNNNNTGG 1 cut(s) 2754
BstYI RGATCY 2 cut(s) 1756, 2297
Bsu15I ATCGAT 1 cut(s) 19
BsuI GTATCC 1 cut(s) 620
BsuTUI ATCGAT 1 cut(s) 19
BtgZI GCGATG 1 cut(s) 2067
BtsCI GGATG 7 cut(s) 49, 733, 1284, 1495, 1630, 1786, 2164
BtsI GCAGTG 2 cut(s) 824, 2049
BveI ACCTGC 2 cut(s) 68, 820
Cac8I GCNNGC 3 cut(s) 2737, 2756, 2798
CaiI CAGNNNCTG 1 cut(s) 2048
CfoI GCGC 2 cut(s) 1425, 1767
Cfr13I GGNCC 6 cut(s) 592, 1972, 2058, 2347, 2406, 2865
ClaI ATCGAT 1 cut(s) 19
CseI GACGC 3 cut(s) 1060, 2075, 2665
Csp6I GTAC 3 cut(s) 1544, 1817, 2209
CviQI GTAC 3 cut(s) 1544, 1817, 2209
DpnI GATC 7 cut(s) 18, 22, 947, 1494, 1758, 2299, 2788
DpnII GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
DraI TTTAAA 1 cut(s) 1842
EaeI YGGCCR 4 cut(s) 121, 377, 2187, 2464
Eam1104I CTCTTC 2 cut(s) 684, 1699
EarI CTCTTC 2 cut(s) 684, 1699
Eco105I TACGTA 1 cut(s) 2212
Eco130I CCWWGG 5 cut(s) 2001, 2130, 2439, 2491, 2671
Eco147I AGGCCT 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 812
Eco47I GGWCC 4 cut(s) 592, 2058, 2347, 2865
Eco57I CTGAAG 3 cut(s) 81, 1668, 2571
Eco72I CACGTG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 2293
EcoNI CCTNNNNNAGG 2 cut(s) 1292, 1604
EcoO109I RGGNCCY 1 cut(s) 2406
EcoRI GAATTC 2 cut(s) 156, 1959
EcoRII CCWGG 4 cut(s) 34, 268, 1768, 2592
EcoT14I CCWWGG 5 cut(s) 2001, 2130, 2439, 2491, 2671
EcoT22I ATGCAT 2 cut(s) 6, 150
ErhI CCWWGG 5 cut(s) 2001, 2130, 2439, 2491, 2671
FalI AAGNNNNNCTT 2 cut(s) 1985, 2017
FaqI GGGAC 5 cut(s) 605, 1180, 1372, 2071, 2211
Fnu4HI GCNGC 3 cut(s) 1047, 1803, 1873
FokI GGATG 7 cut(s) 56, 740, 1271, 1482, 1637, 1793, 2171
Fsp4HI GCNGC 3 cut(s) 1047, 1803, 1873
FspBI CTAG 7 cut(s) 191, 519, 1140, 1601, 2558, 2672, 2736
FspI TGCGCA 1 cut(s) 1424
GlaI GCGC 2 cut(s) 1424, 1766
GluI GCNGC 3 cut(s) 1047, 1803, 1873
GsuI CTGGAG 1 cut(s) 1293
HapII CCGG 1 cut(s) 2662
HgaI GACGC 3 cut(s) 1060, 2075, 2665
HhaI GCGC 2 cut(s) 1425, 1767
Hin1I GRCGYC 1 cut(s) 2676
Hin6I GCGC 2 cut(s) 1423, 1765
HinP1I GCGC 2 cut(s) 1423, 1765
HincII GTYRAC 5 cut(s) 891, 1195, 2244, 2251, 2335
HindII GTYRAC 5 cut(s) 891, 1195, 2244, 2251, 2335
HindIII AAGCTT 2 cut(s) 652, 2428
HinfI GANTC 4 cut(s) 647, 1583, 1691, 2423
HpaI GTTAAC 2 cut(s) 1195, 2251
HpaII CCGG 1 cut(s) 2662
HphI GGTGA 6 cut(s) 26, 674, 760, 1844, 1978, 2042
Hpy166II GTNNAC 8 cut(s) 891, 1195, 1394, 2209, 2215, 2244, 2251, 2335
Hpy188III TCNNGA 9 cut(s) 624, 711, 1028, 1272, 1931, 2295, 2558, 2818, 2855
Hpy8I GTNNAC 8 cut(s) 891, 1195, 1394, 2209, 2215, 2244, 2251, 2335
Hpy99I CGWCG 4 cut(s) 1054, 2069, 2681, 2849
HpyAV CCTTC 4 cut(s) 191, 1025, 1898, 2707
HpyCH4III ACNGT 6 cut(s) 757, 1816, 2248, 2566, 2692, 2743
HpyCH4IV ACGT 4 cut(s) 116, 2200, 2211, 2253
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 249, 835, 1649
HpySE526I ACGT 4 cut(s) 116, 2200, 2211, 2253
Hsp92I GRCGYC 1 cut(s) 2676
HspAI GCGC 2 cut(s) 1423, 1765
KspAI GTTAAC 2 cut(s) 1195, 2251
Kzo9I GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
LmnI GCTCC 4 cut(s) 1274, 2074, 2308, 2726
Lsp1109I GCAGC 2 cut(s) 1058, 1789
MaeI CTAG 7 cut(s) 191, 519, 1140, 1601, 2558, 2672, 2736
MaeII ACGT 4 cut(s) 116, 2200, 2211, 2253
MalI GATC 7 cut(s) 18, 22, 947, 1494, 1758, 2299, 2788
MboI GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
MfeI CAATTG 2 cut(s) 585, 2321
MflI RGATCY 2 cut(s) 1756, 2297
MhlI GDGCHC 2 cut(s) 1099, 2817
MlsI TGGCCA 4 cut(s) 123, 379, 2189, 2466
MluNI TGGCCA 4 cut(s) 123, 379, 2189, 2466
MlyI GAGTC 2 cut(s) 656, 1685
MmeI TCCRAC 2 cut(s) 337, 544
Mox20I TGGCCA 4 cut(s) 123, 379, 2189, 2466
Mph1103I ATGCAT 2 cut(s) 6, 150
MscI TGGCCA 4 cut(s) 123, 379, 2189, 2466
MslI CAYNNNNRTG 3 cut(s) 1500, 2342, 2642
Msp20I TGGCCA 4 cut(s) 123, 379, 2189, 2466
MspI CCGG 1 cut(s) 2662
MspR9I CCNGG 5 cut(s) 36, 270, 1770, 2594, 2663
MunI CAATTG 2 cut(s) 585, 2321
Mva1269I GAATGC 1 cut(s) 1874
MvaI CCWGG 4 cut(s) 36, 270, 1770, 2594
MwoI GCNNNNNNNGC 4 cut(s) 74, 249, 835, 1649
NciI CCSGG 1 cut(s) 2663
NdeII GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
NheI GCTAGC 1 cut(s) 2735
NlaIV GGNNCC 7 cut(s) 593, 1758, 2059, 2349, 2408, 2837, 2866
NmuCI GTSAC 7 cut(s) 449, 514, 748, 1825, 1832, 2044, 2618
NsbI TGCGCA 1 cut(s) 1424
NsiI ATGCAT 2 cut(s) 6, 150
NspI RCATGY 5 cut(s) 1122, 1927, 2289, 2653, 2800
PaeI GCATGC 1 cut(s) 2800
PasI CCCWGGG 1 cut(s) 1769
PceI AGGCCT 1 cut(s) 137
PciI ACATGT 1 cut(s) 1923
PcsI WCGNNNNNNNCGW 1 cut(s) 2853
PctI GAATGC 1 cut(s) 1874
PfeI GAWTC 2 cut(s) 1583, 2423
PflMI CCANNNNNTGG 3 cut(s) 608, 1721, 2390
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 3 cut(s) 1048, 1804, 1874
PleI GAGTC 2 cut(s) 655, 1685
PmaCI CACGTG 1 cut(s) 117
PmlI CACGTG 1 cut(s) 117
PpsI GAGTC 2 cut(s) 655, 1685
Ppu21I YACGTR 2 cut(s) 117, 2212
PscI ACATGT 1 cut(s) 1923
PshBI ATTAAT 3 cut(s) 317, 1082, 1632
Psp1406I AACGTT 1 cut(s) 2253
Psp6I CCWGG 4 cut(s) 34, 268, 1768, 2592
PspCI CACGTG 1 cut(s) 117
PspGI CCWGG 4 cut(s) 34, 268, 1768, 2592
PspN4I GGNNCC 7 cut(s) 593, 1758, 2059, 2349, 2408, 2837, 2866
PspPI GGNCC 6 cut(s) 592, 1972, 2058, 2347, 2406, 2865
PstI CTGCAG 1 cut(s) 831
PstNI CAGNNNCTG 1 cut(s) 2048
PsuI RGATCY 2 cut(s) 1756, 2297
RsaI GTAC 3 cut(s) 1545, 1818, 2210
RsaNI GTAC 3 cut(s) 1544, 1817, 2209
RseI CAYNNNNRTG 3 cut(s) 1500, 2342, 2642
SatI GCNGC 3 cut(s) 1047, 1803, 1873
Sau3AI GATC 7 cut(s) 16, 20, 945, 1492, 1756, 2297, 2786
Sau96I GGNCC 6 cut(s) 592, 1972, 2058, 2347, 2406, 2865
ScaI AGTACT 1 cut(s) 1545
SchI GAGTC 2 cut(s) 656, 1685
ScrFI CCNGG 5 cut(s) 36, 270, 1770, 2594, 2663
SduI GDGCHC 2 cut(s) 1099, 2817
SfcI CTRYAG 3 cut(s) 454, 827, 2175
SinI GGWCC 4 cut(s) 592, 2058, 2347, 2865
SmiI ATTTAAAT 1 cut(s) 1842
SmiMI CAYNNNNRTG 3 cut(s) 1500, 2342, 2642
SmlI CTYRAG 2 cut(s) 1654, 2816
SmoI CTYRAG 2 cut(s) 1654, 2816
SnaBI TACGTA 1 cut(s) 2212
SphI GCATGC 1 cut(s) 2800
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 6 cut(s) 250, 971, 1001, 1872, 2839, 2884
SspI AATATT 2 cut(s) 1254, 1438
SspMI CTAG 7 cut(s) 191, 519, 1140, 1601, 2558, 2672, 2736
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 5 cut(s) 34, 268, 1768, 2592, 2661
StyI CCWWGG 5 cut(s) 2001, 2130, 2439, 2491, 2671
SwaI ATTTAAAT 1 cut(s) 1842
TaaI ACNGT 6 cut(s) 757, 1816, 2248, 2566, 2692, 2743
TaiI ACGT 4 cut(s) 119, 2203, 2214, 2256
TaqI TCGA 3 cut(s) 19, 1701, 2856
TatI WGTACW 1 cut(s) 1543
TauI GCSGC 1 cut(s) 1875
TfiI GAWTC 2 cut(s) 1583, 2423
TseFI GTSAC 7 cut(s) 449, 514, 748, 1825, 1832, 2044, 2618
TseI GCWGC 2 cut(s) 1046, 1802
Tsp45I GTSAC 7 cut(s) 449, 514, 748, 1825, 1832, 2044, 2618
TspGWI ACGGA 2 cut(s) 272, 1637
Van91I CCANNNNNTGG 3 cut(s) 608, 1721, 2390
VpaK11BI GGWCC 4 cut(s) 592, 2058, 2347, 2865
VspI ATTAAT 3 cut(s) 317, 1082, 1632
XagI CCTNNNNNAGG 2 cut(s) 1292, 1604
XapI RAATTY 6 cut(s) 156, 405, 1385, 1562, 1959, 2024
XbaI TCTAGA 1 cut(s) 2557
XceI RCATGY 5 cut(s) 1122, 1927, 2289, 2653, 2800
XcmI CCANNNNNNNNNTGG 3 cut(s) 553, 584, 1183
XmaJI CCTAGG 1 cut(s) 2671
XspI CTAG 7 cut(s) 191, 519, 1140, 1601, 2558, 2672, 2736
ZrmI AGTACT 1 cut(s) 1545
Zsp2I ATGCAT 2 cut(s) 6, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.