AT3G59300

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002666
Physical Location & Seq
Forward (+)
21918321 .. 21922706
4386 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59300.1

Sequence Viewer

Length: 1380 bp
ATGGTGATCGCGGCTGCTTCCTCTTTTTCCCTCGGTCCATCTCATTGCCATCAATCTTACACGGATGAGTTCTCTTCCTCCATACCTTACAAAAGAACCAGCAATGCAAGAAATCGTGTGTTTGATGGATGTGGCTCTGCTAATCTCTCTGTTTTAAGTTCCAGATGCAAGATTCCTTTTTTTGGATCAGCATTTCATGTATCATCCGGTGGACATGATCTCGGCCTCACGAAAGTTTCGGTTGCTGCTGATTATTCTGATTCGGTTCCTGATTCATCTTTCTATGGATATCATCCTCTAGAAGATTTGAAGCCGTCCAAAAGAGTCCAGGAGACAAAGCTCTCTGCTTCTGAAGTTGCAAGGACTACCGTCGAGGCGAACAGCAGTGCTGTGCTGGTGTTTCCTGGAGCTATTCACTGTGAACCACACGATCATAATTCATGGTCCGAATTTAAGTATGTCATTGATGATTATGGAGATATCTTTTTTGAAATTCCTGATGATGAGAACATCTTAGAAGATCCTGGAGCTAGTAATCCAGTGAAAGCCTTTTTTGGAATGGATGTCCCGCGATACGAAAACACACGGCACCATGAGGAATATAACATTTCAGACATTGGTAATCTTGACCAAATTATTTTTGATGACCATTATTTCGAGATTATGGATTCTGAAGCTAGAGATATTCCGATAGATTGGGGAATGCCTGACACCTCCAACGGTGTTCATCCTATTTACTTTGCCAAACACCTGTCGAAGGCCATCAGCATGGACTATGACAGGAAAATGGACTATCCATCTAATGGTGTATCCATACTTGGATGCCTTAGGCCCGCTTTCCTTGACGAGGAATCTTATATAAGAAGATTATTTCTTTCGGAAGATAGAGATGACTATAGCTGGGAGGTTCAAGGTGATGATAATCCAATCACTAGTTCCAGACGTGACGAGAATGATATGAGTTCAAGTCTATATAGATTAGAGATTGTGGGAATAGAGCTTCTCTCCCTCTATGGAGCAGAGTCTTCTATAAGTTTGCAAGATTTTCAAGATGCTGAACCAGACATCCTAGTGCACTCTACTTCTGCAATCATCGAGCGCTTCAACAACAGGGGGATTAACTCCAGCATTGCACTTAAAGCTCTCTGCAAAAAGAAGGGTCTTCATGCAGAGGAAGCTAACCTGATCAGTGTTGATAGTCTTGGTATGGATGTGAGAGTTTTCGCAGGTGCACAAGTCCAAACTCACCGTTTCCCTTTCAAAACCAGGGCTACAACAGAAATGGCAGCAGAGAAGAAGATCCACCAGCTACTGTTCCCTCGTTCACGCAGAAGGAAGTTGAAGTGTCATGACGAGAGTTTGAAGGATGCATTTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

459

Amino Acids

51.59

Weight (kDa)

5.18

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1217
Acc36I ACCTGC 1 cut(s) 1217
AccB1I GGYRCC 1 cut(s) 588
AccB7I CCANNNNNTGG 1 cut(s) 803
AccII CGCG 2 cut(s) 11, 571
AciI CCGC 3 cut(s) 11, 569, 834
AclWI GGATC 3 cut(s) 193, 515, 1294
AcsI RAATTY 2 cut(s) 449, 492
AcuI CTGAAG 2 cut(s) 372, 693
AfeI AGCGCT 1 cut(s) 1100
AfiI CCNNNNNNNGG 4 cut(s) 182, 757, 803, 847
AgsI TTSAA 9 cut(s) 310, 491, 911, 966, 1049, 1105, 1261, 1342, 1363
AhlI ACTAGT 1 cut(s) 932
AjiI CACGTC 1 cut(s) 944
AjnI CCWGG 4 cut(s) 327, 403, 523, 1265
AjuI GAANNNNNNNTTGG 2 cut(s) 919, 951
AloI GAACNNNNNNTCC 2 cut(s) 1050, 1082
AluBI AGCT 9 cut(s) 340, 410, 530, 677, 900, 1000, 1142, 1178, 1309
AluI AGCT 9 cut(s) 340, 410, 530, 677, 900, 1000, 1142, 1178, 1309
Alw21I GWGCWC 2 cut(s) 1077, 1234
Alw26I GTCTC 1 cut(s) 326
Alw44I GTGCAC 2 cut(s) 1073, 1230
AlwI GGATC 3 cut(s) 193, 515, 1294
AlwNI CAGNNNCTG 1 cut(s) 1312
Aor51HI AGCGCT 1 cut(s) 1100
AoxI GGCC 3 cut(s) 223, 759, 830
ApaLI GTGCAC 2 cut(s) 1073, 1230
ApeKI GCWGC 3 cut(s) 14, 245, 1286
ApoI RAATTY 2 cut(s) 449, 492
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
AspLEI GCGC 1 cut(s) 1101
AspS9I GGNCC 3 cut(s) 35, 444, 831
AsuHPI GGTGA 3 cut(s) 16, 926, 1238
AvaII GGWCC 2 cut(s) 35, 444
AxyI CCTNAGG 1 cut(s) 827
BaeGI GKGCMC 2 cut(s) 1077, 1234
BanI GGYRCC 1 cut(s) 588
BbsI GAAGAC 2 cut(s) 1017, 1154
Bbv12I GWGCWC 2 cut(s) 1077, 1234
BbvI GCAGC 2 cut(s) 232, 1298
BccI CCATC 5 cut(s) 46, 57, 119, 770, 805
BceAI ACGGC 2 cut(s) 298, 602
BciT130I CCWGG 4 cut(s) 329, 405, 525, 1267
BciVI GTATCC 1 cut(s) 820
BclI TGATCA 1 cut(s) 1185
BcoDI GTCTC 1 cut(s) 326
BcuI ACTAGT 1 cut(s) 932
BfaI CTAG 5 cut(s) 299, 531, 678, 933, 1070
BfmI CTRYAG 1 cut(s) 895
BfoI RGCGCY 1 cut(s) 1102
BfuAI ACCTGC 1 cut(s) 1217
BfuI GTATCC 1 cut(s) 820
BisI GCNGC 4 cut(s) 12, 15, 246, 1287
BlsI GCNGC 4 cut(s) 13, 16, 247, 1288
Bme1390I CCNGG 4 cut(s) 329, 405, 525, 1267
Bme18I GGWCC 2 cut(s) 35, 444
BmgBI CACGTC 1 cut(s) 944
BmgT120I GGNCC 3 cut(s) 35, 444, 831
BmiI GGNNCC 2 cut(s) 267, 590
BmrFI CCNGG 4 cut(s) 329, 405, 525, 1267
BmsI GCATC 4 cut(s) 155, 812, 1042, 1357
BoxI GACNNNNGTC 1 cut(s) 368
BpiI GAAGAC 2 cut(s) 1017, 1154
BplI GAGNNNNNCTC 2 cut(s) 989, 1021
BpmI CTGGAG 3 cut(s) 426, 546, 1108
BsaBI GATNNNNATC 1 cut(s) 921
BsaJI CCNNGG 2 cut(s) 31, 1266
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 757, 803, 847
Bse1I ACTGG 1 cut(s) 539
Bse21I CCTNAGG 1 cut(s) 827
Bse3DI GCAATG 3 cut(s) 43, 109, 1128
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 4 cut(s) 329, 405, 525, 1267
BseDI CCNNGG 2 cut(s) 31, 1266
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 4 cut(s) 182, 757, 803, 847
BseMI GCAATG 3 cut(s) 43, 109, 1128
BseNI ACTGG 1 cut(s) 539
BseSI GKGCMC 2 cut(s) 1077, 1234
BseXI GCAGC 2 cut(s) 232, 1298
BseYI CCCAGC 1 cut(s) 900
Bsh1236I CGCG 2 cut(s) 11, 571
BshFI GGCC 3 cut(s) 225, 761, 832
BshNI GGYRCC 1 cut(s) 588
BsiHKAI GWGCWC 2 cut(s) 1077, 1234
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 4 cut(s) 182, 757, 803, 847
BsmAI GTCTC 1 cut(s) 326
BsmFI GGGAC 1 cut(s) 551
BsmI GAATGC 1 cut(s) 708
BsnI GGCC 3 cut(s) 225, 761, 832
Bsp1286I GDGCHC 2 cut(s) 1077, 1234
Bsp143I GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
BspACI CCGC 3 cut(s) 11, 569, 834
BspANI GGCC 3 cut(s) 225, 761, 832
BspFNI CGCG 2 cut(s) 11, 571
BspHI TCATGA 1 cut(s) 1348
BspLI GGNNCC 2 cut(s) 267, 590
BspMI ACCTGC 1 cut(s) 1217
BspPI GGATC 3 cut(s) 193, 515, 1294
BspT107I GGYRCC 1 cut(s) 588
BsrDI GCAATG 3 cut(s) 43, 109, 1128
BsrI ACTGG 1 cut(s) 539
BssECI CCNNGG 2 cut(s) 31, 1266
BssMI GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
Bst2UI CCWGG 4 cut(s) 329, 405, 525, 1267
Bst4CI ACNGT 5 cut(s) 370, 419, 722, 1250, 1314
Bst6I CTCTTC 1 cut(s) 79
BstC8I GCNNGC 1 cut(s) 834
BstDEI CTNAG 2 cut(s) 514, 827
BstENI CCTNNNNNAGG 2 cut(s) 755, 845
BstFNI CGCG 2 cut(s) 11, 571
BstH2I RGCGCY 1 cut(s) 1102
BstHHI GCGC 1 cut(s) 1101
BstKTI GATC 7 cut(s) 9, 188, 220, 433, 523, 1188, 1302
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
BstMWI GCNNNNNNNGC 2 cut(s) 1139, 1175
BstNI CCWGG 4 cut(s) 329, 405, 525, 1267
BstPAI GACNNNNGTC 1 cut(s) 368
BstSCI CCNGG 4 cut(s) 327, 403, 523, 1265
BstSFI CTRYAG 1 cut(s) 895
BstSLI GKGCMC 2 cut(s) 1077, 1234
BstUI CGCG 2 cut(s) 11, 571
BstV1I GCAGC 2 cut(s) 232, 1298
BstV2I GAAGAC 2 cut(s) 1017, 1154
BstX2I RGATCY 2 cut(s) 520, 1299
BstXI CCANNNNNNTGG 1 cut(s) 769
BstYI RGATCY 2 cut(s) 520, 1299
Bsu36I CCTNAGG 1 cut(s) 827
BsuI GTATCC 1 cut(s) 820
BsuRI GGCC 3 cut(s) 225, 761, 832
BtrI CACGTC 1 cut(s) 944
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 4 cut(s) 391, 415, 546, 1195
BveI ACCTGC 1 cut(s) 1217
Cac8I GCNNGC 1 cut(s) 834
CaiI CAGNNNCTG 1 cut(s) 1312
CciI TCATGA 1 cut(s) 1348
CfoI GCGC 1 cut(s) 1101
Cfr13I GGNCC 3 cut(s) 35, 444, 831
CviAII CATG 7 cut(s) 197, 215, 441, 593, 769, 1166, 1349
DdeI CTNAG 2 cut(s) 514, 827
DpnI GATC 7 cut(s) 8, 187, 219, 432, 522, 1187, 1301
DpnII GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
Eco32I GATATC 2 cut(s) 290, 481
Eco47I GGWCC 2 cut(s) 35, 444
Eco47III AGCGCT 1 cut(s) 1100
Eco57I CTGAAG 2 cut(s) 372, 693
Eco81I CCTNAGG 1 cut(s) 827
EcoNI CCTNNNNNAGG 2 cut(s) 755, 845
EcoRII CCWGG 4 cut(s) 327, 403, 523, 1265
EcoRV GATATC 2 cut(s) 290, 481
EcoT22I ATGCAT 1 cut(s) 1372
FaeI CATG 7 cut(s) 200, 218, 444, 596, 772, 1169, 1352
FaqI GGGAC 1 cut(s) 551
FatI CATG 7 cut(s) 196, 214, 440, 592, 768, 1165, 1348
FauI CCCGC 2 cut(s) 576, 841
FbaI TGATCA 1 cut(s) 1185
Fnu4HI GCNGC 4 cut(s) 12, 15, 246, 1287
FokI GGATG 9 cut(s) 77, 141, 190, 279, 575, 714, 834, 1052, 1223
Fsp4HI GCNGC 4 cut(s) 12, 15, 246, 1287
FspBI CTAG 5 cut(s) 299, 531, 678, 933, 1070
GlaI GCGC 1 cut(s) 1100
GluI GCNGC 4 cut(s) 12, 15, 246, 1287
GsaI CCCAGC 1 cut(s) 904
GsuI CTGGAG 3 cut(s) 426, 546, 1108
HaeII RGCGCY 1 cut(s) 1102
HaeIII GGCC 3 cut(s) 225, 761, 832
HapII CCGG 1 cut(s) 207
HhaI GCGC 1 cut(s) 1101
Hin1II CATG 7 cut(s) 200, 218, 444, 596, 772, 1169, 1352
Hin6I GCGC 1 cut(s) 1099
HinP1I GCGC 1 cut(s) 1099
HinfI GANTC 7 cut(s) 172, 260, 272, 324, 668, 851, 1022
HpaII CCGG 1 cut(s) 207
HphI GGTGA 3 cut(s) 16, 926, 1238
Hpy166II GTNNAC 5 cut(s) 212, 422, 1075, 1232, 1325
Hpy188I TCNGA 7 cut(s) 259, 352, 448, 613, 673, 690, 880
Hpy8I GTNNAC 5 cut(s) 212, 422, 1075, 1232, 1325
Hpy99I CGWCG 1 cut(s) 374
HpyAV CCTTC 4 cut(s) 751, 1150, 1326, 1357
HpyCH4III ACNGT 5 cut(s) 370, 419, 722, 1250, 1314
HpyCH4IV ACGT 1 cut(s) 943
HpyF10VI GCNNNNNNNGC 2 cut(s) 1139, 1175
HpyF3I CTNAG 2 cut(s) 514, 827
HpySE526I ACGT 1 cut(s) 943
Hsp92II CATG 7 cut(s) 200, 218, 444, 596, 772, 1169, 1352
HspAI GCGC 1 cut(s) 1099
Ksp22I TGATCA 1 cut(s) 1185
Kzo9I GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
LmnI GCTCC 3 cut(s) 407, 527, 1016
Lsp1109I GCAGC 2 cut(s) 232, 1298
LweI GCATC 4 cut(s) 155, 812, 1042, 1357
MaeI CTAG 5 cut(s) 299, 531, 678, 933, 1070
MaeII ACGT 1 cut(s) 943
MaeIII GTNAC 1 cut(s) 944
MalI GATC 7 cut(s) 8, 187, 219, 432, 522, 1187, 1301
MboI GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
MboII GAAGA 9 cut(s) 66, 314, 530, 876, 893, 1017, 1154, 1306, 1309
MflI RGATCY 2 cut(s) 520, 1299
MhlI GDGCHC 2 cut(s) 1077, 1234
MluCI AATT 4 cut(s) 436, 449, 492, 633
MlyI GAGTC 2 cut(s) 333, 1031
MmeI TCCRAC 1 cut(s) 741
Mph1103I ATGCAT 1 cut(s) 1372
MseI TTAA 5 cut(s) 155, 453, 1119, 1137, 1378
MslI CAYNNNNRTG 2 cut(s) 767, 1070
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 4 cut(s) 329, 405, 525, 1267
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 4 cut(s) 329, 405, 525, 1267
MvnI CGCG 2 cut(s) 11, 571
MwoI GCNNNNNNNGC 2 cut(s) 1139, 1175
NdeII GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
NlaIII CATG 7 cut(s) 200, 218, 444, 596, 772, 1169, 1352
NlaIV GGNNCC 2 cut(s) 267, 590
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 944
NsiI ATGCAT 1 cut(s) 1372
PagI TCATGA 1 cut(s) 1348
PaqCI CACCTGC 1 cut(s) 1217
PctI GAATGC 1 cut(s) 708
PfeI GAWTC 5 cut(s) 172, 260, 272, 668, 851
PflMI CCANNNNNTGG 1 cut(s) 803
PfoI TCCNGGA 3 cut(s) 327, 403, 523
PkrI GCNGC 4 cut(s) 13, 16, 247, 1288
PleI GAGTC 2 cut(s) 332, 1030
PpsI GAGTC 2 cut(s) 332, 1030
PshAI GACNNNNGTC 1 cut(s) 368
Psp6I CCWGG 4 cut(s) 327, 403, 523, 1265
PspFI CCCAGC 1 cut(s) 900
PspGI CCWGG 4 cut(s) 327, 403, 523, 1265
PspN4I GGNNCC 2 cut(s) 267, 590
PspPI GGNCC 3 cut(s) 35, 444, 831
PstNI CAGNNNCTG 1 cut(s) 1312
PsuI RGATCY 2 cut(s) 520, 1299
RseI CAYNNNNRTG 2 cut(s) 767, 1070
SaqAI TTAA 5 cut(s) 155, 453, 1119, 1137, 1378
SatI GCNGC 4 cut(s) 12, 15, 246, 1287
Sau3AI GATC 7 cut(s) 6, 185, 217, 430, 520, 1185, 1299
Sau96I GGNCC 3 cut(s) 35, 444, 831
SchI GAGTC 2 cut(s) 333, 1031
ScrFI CCNGG 4 cut(s) 329, 405, 525, 1267
SduI GDGCHC 2 cut(s) 1077, 1234
SfaNI GCATC 4 cut(s) 155, 812, 1042, 1357
SfcI CTRYAG 1 cut(s) 895
SinI GGWCC 2 cut(s) 35, 444
SmiMI CAYNNNNRTG 2 cut(s) 767, 1070
SpeI ACTAGT 1 cut(s) 932
Sse9I AATT 4 cut(s) 436, 449, 492, 633
SsiI CCGC 3 cut(s) 11, 569, 834
SspMI CTAG 5 cut(s) 299, 531, 678, 933, 1070
StyD4I CCNGG 4 cut(s) 327, 403, 523, 1265
TaaI ACNGT 5 cut(s) 370, 419, 722, 1250, 1314
TaiI ACGT 1 cut(s) 946
TaqI TCGA 4 cut(s) 372, 657, 755, 1095
TaqII GACCGA 1 cut(s) 23
TasI AATT 4 cut(s) 436, 449, 492, 633
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 5 cut(s) 172, 260, 272, 668, 851
Tru1I TTAA 5 cut(s) 155, 453, 1119, 1137, 1378
Tru9I TTAA 5 cut(s) 155, 453, 1119, 1137, 1378
TscAI CASTG 4 cut(s) 391, 422, 546, 1195
TseFI GTSAC 1 cut(s) 944
TseI GCWGC 3 cut(s) 14, 245, 1286
Tsp45I GTSAC 1 cut(s) 944
TspDTI ATGAA 5 cut(s) 185, 264, 429, 716, 1154
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 4 cut(s) 391, 422, 546, 1195
Van91I CCANNNNNTGG 1 cut(s) 803
VneI GTGCAC 2 cut(s) 1073, 1230
VpaK11BI GGWCC 2 cut(s) 35, 444
XagI CCTNNNNNAGG 2 cut(s) 755, 845
XapI RAATTY 2 cut(s) 449, 492
XbaI TCTAGA 1 cut(s) 298
XspI CTAG 5 cut(s) 299, 531, 678, 933, 1070
Zsp2I ATGCAT 1 cut(s) 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.