Rmu_sc0005878.1_g000022

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005878.1
OG0002666
Physical Location & Seq
Forward (+)
89050 .. 97200
8151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005878.1_g000022.1.cds

Sequence Viewer

Length: 1545 bp
atggctgctgatgctggggcagtagcgcggagggagatgtacgcgcagagggggggggggggcgggggggggaggaggaggcgcggggctgctggaaccagcgctcggcctggtgtgctggtctggccagctgtggggcttcaaccagcgattccgccaccgactgcttcttctcttcccttgggtcattcacattgccattcatgccacacagaaggagtttgttgttcgacaagacatgggatctcaagcagttggatgaaacctccttttgatggtcgaagatcccctgatcctctgggtgtaagtttgaagtgcaggaatccgttagttggaccaacgcaattccactggctgtctattggacatggtctttgcctttccaaagtttcagttgctgcggatttctcagattctgctcctgaatcttctagttatatgactaaccagggctatcatcctcttgaggagttgaaagcgtgcaaaatggtccgagacactaaactcacctgtgctgaaatagcaagaacaacagtcgaggctaacgacagtgctttgctggttttccctgggaagatacactctgaaccacatgaacaaatttcttgggctgagtttcaatatgttatcgacgattatggagatttatattttgaactgtttgatgatgcgaacatattagaagatcctacagcaagtaaccctgtgaatgctttgtttggaatggatatccctgtacacaacaatggaagaataactggaggattcagcattcaagatgaccagaacagtgacgatattccttttgatgatgattatctagaggttgtagaatctgaagcttttgatgttctagactgggagatacctgatgcttctaccttgattcatcccatttattttgcaaagtgcttaacaaaggccatcaatatcagacatgacagaaaaatggatcacccattaaatggtgtttctattttaggatgcctcatacctgcttttgctgatgaagaattttatgtaagaagactatttcattatgaggatagcgactattacaactcggatgagaaaggtggaaaaggagtgagcattagttccaaaagtgaccgaagcaagaattgctcaacactctaccggttggagataatgagaatagagttgttctcggtatatggtgttcagtccacaattaacctacaagattttcaagatgcggaacctgattttcttataaactctatctcggatattgtagaacggttcaacgaaagaggtattagatgcgatgttgctcttaaagctttatgcaaaagaaaaggacttcaagttgagggagctcacttgattggggttgacagtctaggaatggatgttagagtcttctcaggttcagaagtacaaactcatcgttttcccttcagagtccgggccaagtctgaagttgcagctgaaaaacagattgagcaacttttgttcccacgctctcgccgtaaaaagttgagaagtcaaggaaataattttcagggtgtggagtcgtactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

514

Amino Acids

56.97

Weight (kDa)

5.67

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1235
Acc36I ACCTGC 1 cut(s) 1003
AccB7I CCANNNNNTGG 1 cut(s) 965
AccII CGCG 3 cut(s) 28, 44, 84
AciI CCGC 6 cut(s) 28, 63, 84, 155, 401, 1217
AclWI GGATC 5 cut(s) 251, 279, 287, 680, 960
AcoI YGGCCR 1 cut(s) 125
AcsI RAATTY 2 cut(s) 600, 1013
AcuI CTGAAG 3 cut(s) 858, 1405, 1461
AfaI GTAC 4 cut(s) 41, 738, 1401, 1541
AfeI AGCGCT 1 cut(s) 103
AfiI CCNNNNNNNGG 5 cut(s) 105, 134, 275, 332, 965
AgeI ACCGGT 1 cut(s) 1137
AgsI TTSAA 9 cut(s) 143, 313, 475, 620, 656, 776, 1211, 1267, 1328
AjnI CCWGG 3 cut(s) 109, 447, 568
AluBI AGCT 5 cut(s) 131, 842, 1304, 1340, 1451
AluI AGCT 5 cut(s) 131, 842, 1304, 1340, 1451
Alw21I GWGCWC 1 cut(s) 1342
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 5 cut(s) 251, 279, 287, 680, 960
AlwNI CAGNNNCTG 2 cut(s) 398, 416
Aor51HI AGCGCT 1 cut(s) 103
AoxI GGCC 4 cut(s) 107, 125, 921, 1431
ApeKI GCWGC 4 cut(s) 5, 89, 398, 1448
ApoI RAATTY 2 cut(s) 600, 1013
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AsiGI ACCGGT 1 cut(s) 1137
AspLEI GCGC 4 cut(s) 28, 46, 84, 104
AspS9I GGNCC 3 cut(s) 335, 490, 1431
AsuC2I CCSGG 1 cut(s) 1430
AsuHPI GGTGA 2 cut(s) 499, 947
AvaII GGWCC 2 cut(s) 335, 490
BalI TGGCCA 1 cut(s) 127
BanII GRGCYC 1 cut(s) 1342
BbsI GAAGAC 2 cut(s) 1033, 1375
Bbv12I GWGCWC 1 cut(s) 1342
BbvI GCAGC 3 cut(s) 76, 385, 1460
BccI CCATC 2 cut(s) 269, 932
BceAI ACGGC 1 cut(s) 1476
BciT130I CCWGG 3 cut(s) 111, 449, 570
BcnI CCSGG 1 cut(s) 1430
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 4 cut(s) 432, 821, 854, 1364
BfmI CTRYAG 1 cut(s) 690
BfoI RGCGCY 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 1003
BisI GCNGC 4 cut(s) 6, 90, 399, 1449
BlsI GCNGC 4 cut(s) 7, 91, 400, 1450
Bme1390I CCNGG 4 cut(s) 111, 449, 570, 1430
Bme18I GGWCC 2 cut(s) 335, 490
BmgT120I GGNCC 3 cut(s) 335, 490, 1431
BmiI GGNNCC 2 cut(s) 97, 1221
BmrFI CCNGG 4 cut(s) 111, 449, 570, 1430
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 5 cut(s) 658, 862, 974, 1204, 1274
BmuI ACTGGG 1 cut(s) 868
BpiI GAAGAC 2 cut(s) 1033, 1375
BplI GAGNNNNNCTC 2 cut(s) 1151, 1183
BpmI CTGGAG 1 cut(s) 780
BpuEI CTTGAG 2 cut(s) 232, 485
BpuMI CCSGG 1 cut(s) 1430
BsaBI GATNNNNATC 1 cut(s) 816
BsaJI CCNNGG 4 cut(s) 180, 448, 568, 569
BsaWI WCCGGW 1 cut(s) 1137
Bsc4I CCNNNNNNNGG 5 cut(s) 105, 134, 275, 332, 965
Bse118I RCCGGY 1 cut(s) 1137
Bse1I ACTGG 3 cut(s) 356, 763, 863
Bse3DI GCAATG 1 cut(s) 193
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 3 cut(s) 111, 449, 570
BseDI CCNNGG 4 cut(s) 180, 448, 568, 569
BseGI GGATG 6 cut(s) 264, 457, 889, 989, 1072, 1378
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 5 cut(s) 105, 134, 275, 332, 965
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 3 cut(s) 423, 603, 1401
BseNI ACTGG 3 cut(s) 356, 763, 863
BseRI GAGGAG 3 cut(s) 88, 91, 482
BseXI GCAGC 3 cut(s) 76, 385, 1460
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 1 cut(s) 337
Bsh1236I CGCG 3 cut(s) 28, 44, 84
BshFI GGCC 4 cut(s) 109, 127, 923, 1433
BshTI ACCGGT 1 cut(s) 1137
BsiHKAI GWGCWC 1 cut(s) 1342
BsiSI CCGG 2 cut(s) 1138, 1429
BslI CCNNNNNNNGG 5 cut(s) 105, 134, 275, 332, 965
BsmAI GTCTC 1 cut(s) 489
BsmI GAATGC 2 cut(s) 715, 771
BsnI GGCC 4 cut(s) 109, 127, 923, 1433
Bsp1286I GDGCHC 1 cut(s) 1342
Bsp1407I TGTACA 1 cut(s) 736
Bsp143I GATC 5 cut(s) 243, 284, 292, 685, 952
BspACI CCGC 6 cut(s) 28, 63, 84, 155, 401, 1217
BspANI GGCC 4 cut(s) 109, 127, 923, 1433
BspCNI CTCAG 3 cut(s) 422, 604, 1400
BspFNI CGCG 3 cut(s) 28, 44, 84
BspLI GGNNCC 2 cut(s) 97, 1221
BspMI ACCTGC 1 cut(s) 1003
BspPI GGATC 5 cut(s) 251, 279, 287, 680, 960
BsrDI GCAATG 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 1137
BsrGI TGTACA 1 cut(s) 736
BsrI ACTGG 3 cut(s) 356, 763, 863
BssAI RCCGGY 1 cut(s) 1137
BssECI CCNNGG 4 cut(s) 180, 448, 568, 569
BssMI GATC 5 cut(s) 243, 284, 292, 685, 952
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 3 cut(s) 111, 449, 570
Bst4CI ACNGT 6 cut(s) 535, 551, 660, 791, 1263, 1361
Bst6I CTCTTC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 1122
BstAUI TGTACA 1 cut(s) 736
BstC8I GCNNGC 2 cut(s) 129, 481
BstDEI CTNAG 3 cut(s) 409, 612, 1387
BstF5I GGATG 6 cut(s) 264, 457, 889, 989, 1072, 1378
BstFNI CGCG 3 cut(s) 28, 44, 84
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 4 cut(s) 28, 46, 84, 104
BstKTI GATC 5 cut(s) 246, 287, 295, 688, 955
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 5 cut(s) 243, 284, 292, 685, 952
BstMWI GCNNNNNNNGC 7 cut(s) 11, 115, 124, 204, 521, 1122, 1301
BstNI CCWGG 3 cut(s) 111, 449, 570
BstSCI CCNGG 4 cut(s) 109, 447, 568, 1428
BstSFI CTRYAG 1 cut(s) 690
BstUI CGCG 3 cut(s) 28, 44, 84
BstV1I GCAGC 3 cut(s) 76, 385, 1460
BstV2I GAAGAC 2 cut(s) 1033, 1375
BstX2I RGATCY 3 cut(s) 243, 284, 685
BstYI RGATCY 3 cut(s) 243, 284, 685
BsuRI GGCC 4 cut(s) 109, 127, 923, 1433
BtgZI GCGATG 1 cut(s) 1302
BtsCI GGATG 6 cut(s) 264, 457, 889, 989, 1072, 1378
BtsIMutI CAGTG 3 cut(s) 349, 556, 796
BveI ACCTGC 1 cut(s) 1003
Cac8I GCNNGC 2 cut(s) 129, 481
CaiI CAGNNNCTG 2 cut(s) 398, 416
CfoI GCGC 4 cut(s) 28, 46, 84, 104
Cfr10I RCCGGY 1 cut(s) 1137
Cfr13I GGNCC 3 cut(s) 335, 490, 1431
Csp6I GTAC 4 cut(s) 40, 737, 1400, 1540
CspAI ACCGGT 1 cut(s) 1137
CviAII CATG 5 cut(s) 204, 239, 368, 593, 938
CviQI GTAC 4 cut(s) 40, 737, 1400, 1540
DdeI CTNAG 3 cut(s) 409, 612, 1387
DpnI GATC 5 cut(s) 245, 286, 294, 687, 954
DpnII GATC 5 cut(s) 243, 284, 292, 685, 952
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 144
Ecl136II GAGCTC 1 cut(s) 1340
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 1342
Eco32I GATATC 1 cut(s) 730
Eco47I GGWCC 2 cut(s) 335, 490
Eco47III AGCGCT 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 1340
Eco57I CTGAAG 3 cut(s) 858, 1405, 1461
EcoICRI GAGCTC 1 cut(s) 1340
EcoRII CCWGG 3 cut(s) 109, 447, 568
EcoRV GATATC 1 cut(s) 730
EcoT14I CCWWGG 1 cut(s) 180
EcoT38I GRGCYC 1 cut(s) 1342
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 5 cut(s) 207, 242, 371, 596, 941
FatI CATG 5 cut(s) 203, 238, 367, 592, 937
FauI CCCGC 2 cut(s) 56, 77
Fnu4HI GCNGC 4 cut(s) 6, 90, 399, 1449
FokI GGATG 6 cut(s) 271, 444, 876, 996, 1079, 1385
FriOI GRGCYC 1 cut(s) 1342
Fsp4HI GCNGC 4 cut(s) 6, 90, 399, 1449
FspBI CTAG 4 cut(s) 432, 821, 854, 1364
GlaI GCGC 4 cut(s) 27, 45, 83, 103
GluI GCNGC 4 cut(s) 6, 90, 399, 1449
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 780
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 4 cut(s) 109, 127, 923, 1433
HapII CCGG 2 cut(s) 1138, 1429
HhaI GCGC 4 cut(s) 28, 46, 84, 104
Hin1II CATG 5 cut(s) 207, 242, 371, 596, 941
Hin6I GCGC 4 cut(s) 26, 44, 82, 102
HinP1I GCGC 4 cut(s) 26, 44, 82, 102
HincII GTYRAC 1 cut(s) 1357
HindII GTYRAC 1 cut(s) 1357
HindIII AAGCTT 2 cut(s) 840, 1302
HpaII CCGG 2 cut(s) 1138, 1429
HphI GGTGA 2 cut(s) 499, 947
Hpy166II GTNNAC 3 cut(s) 739, 1188, 1357
Hpy188III TCNNGA 6 cut(s) 422, 464, 776, 821, 854, 1211
Hpy8I GTNNAC 3 cut(s) 739, 1188, 1357
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 2 cut(s) 209, 1429
HpyCH4III ACNGT 6 cut(s) 535, 551, 660, 791, 1263, 1361
HpyCH4V TGCA 5 cut(s) 318, 483, 905, 1311, 1448
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 115, 124, 204, 521, 1122, 1301
HpyF3I CTNAG 3 cut(s) 409, 612, 1387
Hsp92II CATG 5 cut(s) 207, 242, 371, 596, 941
HspAI GCGC 4 cut(s) 26, 44, 82, 102
Kzo9I GATC 5 cut(s) 243, 284, 292, 685, 952
LmnI GCTCC 2 cut(s) 424, 1337
Lsp1109I GCAGC 3 cut(s) 76, 385, 1460
LweI GCATC 5 cut(s) 658, 862, 974, 1204, 1274
MaeI CTAG 4 cut(s) 432, 821, 854, 1364
MaeIII GTNAC 3 cut(s) 698, 791, 1106
MalI GATC 5 cut(s) 245, 286, 294, 687, 954
MboI GATC 5 cut(s) 243, 284, 292, 685, 952
MflI RGATCY 3 cut(s) 243, 284, 685
MhlI GDGCHC 1 cut(s) 1342
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 6 cut(s) 344, 600, 1013, 1120, 1191, 1519
MluNI TGGCCA 1 cut(s) 127
MlyI GAGTC 3 cut(s) 1389, 1434, 1544
MmeI TCCRAC 3 cut(s) 236, 313, 1122
Mox20I TGGCCA 1 cut(s) 127
MscI TGGCCA 1 cut(s) 127
MseI TTAA 4 cut(s) 914, 962, 1194, 1299
MslI CAYNNNNRTG 1 cut(s) 744
Msp20I TGGCCA 1 cut(s) 127
MspA1I CMGCKG 2 cut(s) 131, 1451
MspI CCGG 2 cut(s) 1138, 1429
MspR9I CCNGG 4 cut(s) 111, 449, 570, 1430
Mva1269I GAATGC 2 cut(s) 715, 771
MvaI CCWGG 3 cut(s) 111, 449, 570
MvnI CGCG 3 cut(s) 28, 44, 84
MwoI GCNNNNNNNGC 7 cut(s) 11, 115, 124, 204, 521, 1122, 1301
NciI CCSGG 1 cut(s) 1430
NdeII GATC 5 cut(s) 243, 284, 292, 685, 952
NlaIII CATG 5 cut(s) 207, 242, 371, 596, 941
NlaIV GGNNCC 2 cut(s) 97, 1221
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 791, 1106
PasI CCCWGGG 1 cut(s) 569
PcsI WCGNNNNNNNCGW 2 cut(s) 543, 1489
PctI GAATGC 2 cut(s) 715, 771
PfeI GAWTC 7 cut(s) 151, 322, 413, 425, 765, 833, 886
PflFI GACNNNGTC 1 cut(s) 369
PflMI CCANNNNNTGG 1 cut(s) 965
PinAI ACCGGT 1 cut(s) 1137
PkrI GCNGC 4 cut(s) 7, 91, 400, 1450
PleI GAGTC 3 cut(s) 1388, 1433, 1543
PpsI GAGTC 3 cut(s) 1388, 1433, 1543
PsiI TTATAA 1 cut(s) 1235
Psp124BI GAGCTC 1 cut(s) 1342
Psp6I CCWGG 3 cut(s) 109, 447, 568
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 3 cut(s) 109, 447, 568
PspN4I GGNNCC 2 cut(s) 97, 1221
PspPI GGNCC 3 cut(s) 335, 490, 1431
PsrI GAACNNNNNNTAC 2 cut(s) 1164, 1196
PstNI CAGNNNCTG 2 cut(s) 398, 416
PsuI RGATCY 3 cut(s) 243, 284, 685
PsyI GACNNNGTC 1 cut(s) 369
PvuII CAGCTG 2 cut(s) 131, 1451
RsaI GTAC 4 cut(s) 41, 738, 1401, 1541
RsaNI GTAC 4 cut(s) 40, 737, 1400, 1540
RseI CAYNNNNRTG 1 cut(s) 744
SacI GAGCTC 1 cut(s) 1342
SaqAI TTAA 4 cut(s) 914, 962, 1194, 1299
SatI GCNGC 4 cut(s) 6, 90, 399, 1449
Sau3AI GATC 5 cut(s) 243, 284, 292, 685, 952
Sau96I GGNCC 3 cut(s) 335, 490, 1431
SchI GAGTC 3 cut(s) 1389, 1434, 1544
ScrFI CCNGG 4 cut(s) 111, 449, 570, 1430
SduI GDGCHC 1 cut(s) 1342
SfaNI GCATC 5 cut(s) 658, 862, 974, 1204, 1274
SfcI CTRYAG 1 cut(s) 690
SinI GGWCC 2 cut(s) 335, 490
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 2 cut(s) 247, 464
SmoI CTYRAG 2 cut(s) 247, 464
Sse9I AATT 6 cut(s) 344, 600, 1013, 1120, 1191, 1519
SsiI CCGC 6 cut(s) 28, 63, 84, 155, 401, 1217
SspMI CTAG 4 cut(s) 432, 821, 854, 1364
SstI GAGCTC 1 cut(s) 1342
StyD4I CCNGG 4 cut(s) 109, 447, 568, 1428
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 6 cut(s) 535, 551, 660, 791, 1263, 1361
TaqI TCGA 4 cut(s) 230, 280, 537, 630
TaqII GACCGA 1 cut(s) 1125
TasI AATT 6 cut(s) 344, 600, 1013, 1120, 1191, 1519
TatI WGTACW 2 cut(s) 736, 1399
TfiI GAWTC 7 cut(s) 151, 322, 413, 425, 765, 833, 886
Tru1I TTAA 4 cut(s) 914, 962, 1194, 1299
Tru9I TTAA 4 cut(s) 914, 962, 1194, 1299
TscAI CASTG 3 cut(s) 356, 556, 796
TseFI GTSAC 2 cut(s) 791, 1106
TseI GCWGC 4 cut(s) 5, 89, 398, 1448
Tsp45I GTSAC 2 cut(s) 791, 1106
TspDTI ATGAA 6 cut(s) 192, 275, 609, 878, 1023, 1025
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 3 cut(s) 356, 556, 796
Tth111I GACNNNGTC 1 cut(s) 369
Van91I CCANNNNNTGG 1 cut(s) 965
VpaK11BI GGWCC 2 cut(s) 335, 490
XapI RAATTY 2 cut(s) 600, 1013
XbaI TCTAGA 2 cut(s) 820, 853
XspI CTAG 4 cut(s) 432, 821, 854, 1364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.