RchiOBHm_Chr1g0335941

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002666
Physical Location & Seq
Reverse (-)
27624363 .. 27633063
8701 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56398

Sequence Viewer

Length: 1281 bp
ATGGCAATTGCAGCTGCTTCTTCTCTTCCCTTGGGTCATTCGCATTGCCACTCATGCCACACAGAAGGAGTTTGTTGTTCGACAAGACATGGGATCTCAAGCAGTTGGATGAAACCTCCTTTTGATGGTCGAAGATACCCTGATCCTCTGGGTGTAAGTTTGAAGTGCAGGAATCCGTTAGTTGGACCAACACAATTCCACTGGCTGTCTATTGGACATGGTCTTTGCCTTTCCAAAGTTTCGGTTGCTGCGGATTTCTCAGATTCTGCTCCTGAATCTTCTAGTTATATGACTAACCAGGGTTATCATCCTCTTGAGGAGTTGAAAGCGTGCAAAATGGTCCGAGACACTAAACTCACCTCTGCTGAAATAGCAAGAACAACAGTCGAGGCTAACGACAGTGCTTTGCTGGTTTTCCCTGGGAAGATACACTCTGAACCACATGAACAAATTTCTTGGGCTGAGTTTCAATATGTTATCGACGATTATGGAAATGCTTTGTTTGGAATGGATATCCCTACACACAACAATGGAAGAATAGCTGGAGGATTCAGCATTCTAGATGACCAGAACAGTGACGATATTCCTTTTGATGATCATTATCTAGAGGTTGTACAATCTGAAGCTTTTGATGTTCTGGACTGGGAGATACCTGATGCTTCTACCTTGATTCATCCCATTTATTTTGCAAAGTGCTTAACAAAGGCCATCAATATCAGACATGACCGGAAAATGGATCACCCATCAAATGGTGTTTCTATTTTAGGATGCCTCATACCTGCATTTGCTGATGAAGAATTTTATGTAAGAAGACTATTTCATTATGAGGATAGTGACTATTACAACTCGGATGAGAAAGATGGAAAAGGAGTGAGCATTAGTTCCAAAAGTGACCGAAGCAAGAATTGCTTAGCACTCTACCGGTTGGAGATAATGAGAATAGAGTTGTTCTCAGTATATGAACGGTTCAACGAAAGAGGTATTAGATGCGATGTTGCTCTTAAAGCTTTATGCAAAAGAAAAGGACTTCAAGTTGAGGGGGTTGACAGTCTAGGTATGGATGTAGTCTTCTCAGGGTCAGAAGTACAAACTCATCGTTTTCCCTTCAGAGTCCGGGTAAGCAATAAGCAGTTAATCGTATTTAACATAGGTACATACTGTACATGCCACGTCTGGCTATGTACATGGAGTAATGCACTAGGCTTAATAGTTATATTTGATTTTTTACTTGATCCTAATGGTATCATGCTGTTTTCTCCTCACTATGCATGCAATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

47.83

Weight (kDa)

5.72

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 787
AccB7I CCANNNNNTGG 1 cut(s) 749
AciI CCGC 1 cut(s) 251
AclWI GGATC 4 cut(s) 101, 137, 744, 1226
AcsI RAATTY 2 cut(s) 450, 797
AcuI CTGAAG 2 cut(s) 642, 1090
AfaI GTAC 5 cut(s) 615, 1086, 1153, 1162, 1183
AfiI CCNNNNNNNGG 4 cut(s) 125, 182, 733, 749
AgeI ACCGGT 1 cut(s) 921
AgsI TTSAA 5 cut(s) 163, 325, 470, 970, 1031
AjiI CACGTC 1 cut(s) 1171
AjnI CCWGG 2 cut(s) 297, 418
AluBI AGCT 4 cut(s) 14, 542, 626, 1007
AluI AGCT 4 cut(s) 14, 542, 626, 1007
Alw26I GTCTC 1 cut(s) 339
AlwI GGATC 4 cut(s) 101, 137, 744, 1226
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 1 cut(s) 705
ApeKI GCWGC 3 cut(s) 11, 14, 248
ApoI RAATTY 2 cut(s) 450, 797
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AsiGI ACCGGT 1 cut(s) 921
AspS9I GGNCC 2 cut(s) 185, 340
AsuC2I CCSGG 1 cut(s) 1115
AsuHPI GGTGA 2 cut(s) 349, 731
AvaII GGWCC 2 cut(s) 185, 340
BbsI GAAGAC 2 cut(s) 817, 1060
BbvI GCAGC 2 cut(s) 23, 235
BccI CCATC 4 cut(s) 119, 716, 751, 854
BciT130I CCWGG 2 cut(s) 299, 420
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 1 cut(s) 1115
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 5 cut(s) 282, 560, 605, 1052, 1199
BfuAI ACCTGC 1 cut(s) 787
BisI GCNGC 3 cut(s) 12, 15, 249
BlpI GCTNAGC 1 cut(s) 910
BlsI GCNGC 3 cut(s) 13, 16, 250
Bme1390I CCNGG 3 cut(s) 299, 420, 1115
Bme18I GGWCC 2 cut(s) 185, 340
BmgBI CACGTC 1 cut(s) 1171
BmgT120I GGNCC 2 cut(s) 185, 340
BmrFI CCNGG 3 cut(s) 299, 420, 1115
BmrI ACTGGG 1 cut(s) 652
BmsI GCATC 3 cut(s) 646, 758, 977
BmuI ACTGGG 1 cut(s) 652
BpiI GAAGAC 2 cut(s) 817, 1060
BplI GAGNNNNNCTC 2 cut(s) 935, 967
BpmI CTGGAG 1 cut(s) 564
Bpu1102I GCTNAGC 1 cut(s) 910
BpuEI CTTGAG 2 cut(s) 82, 335
BpuMI CCSGG 1 cut(s) 1115
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 4 cut(s) 30, 298, 418, 419
BsaWI WCCGGW 2 cut(s) 726, 921
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 182, 733, 749
Bse118I RCCGGY 1 cut(s) 921
Bse1I ACTGG 2 cut(s) 206, 647
Bse3DI GCAATG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 2 cut(s) 299, 420
BseDI CCNNGG 4 cut(s) 30, 298, 418, 419
BseGI GGATG 6 cut(s) 114, 307, 673, 773, 856, 1066
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 4 cut(s) 125, 182, 733, 749
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 4 cut(s) 273, 453, 966, 1086
BseNI ACTGG 2 cut(s) 206, 647
BseRI GAGGAG 2 cut(s) 332, 1248
BseXI GCAGC 2 cut(s) 23, 235
BsgI GTGCAG 1 cut(s) 187
BshFI GGCC 1 cut(s) 707
BshTI ACCGGT 1 cut(s) 921
BsiSI CCGG 3 cut(s) 727, 922, 1114
BslI CCNNNNNNNGG 4 cut(s) 125, 182, 733, 749
BsmAI GTCTC 1 cut(s) 339
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 1 cut(s) 707
Bsp1407I TGTACA 3 cut(s) 613, 1160, 1181
Bsp143I GATC 5 cut(s) 93, 142, 595, 736, 1231
Bsp1720I GCTNAGC 1 cut(s) 910
BspACI CCGC 1 cut(s) 251
BspANI GGCC 1 cut(s) 707
BspCNI CTCAG 4 cut(s) 272, 454, 965, 1085
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 4 cut(s) 101, 137, 744, 1226
BsrDI GCAATG 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 921
BsrGI TGTACA 3 cut(s) 613, 1160, 1181
BsrI ACTGG 2 cut(s) 206, 647
BssAI RCCGGY 1 cut(s) 921
BssECI CCNNGG 4 cut(s) 30, 298, 418, 419
BssMI GATC 5 cut(s) 93, 142, 595, 736, 1231
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 299, 420
Bst4CI ACNGT 6 cut(s) 385, 401, 575, 966, 1049, 1160
Bst6I CTCTTC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 906
BstAUI TGTACA 3 cut(s) 613, 1160, 1181
BstC8I GCNNGC 2 cut(s) 331, 1270
BstDEI CTNAG 5 cut(s) 259, 462, 910, 952, 1072
BstF5I GGATG 6 cut(s) 114, 307, 673, 773, 856, 1066
BstKTI GATC 5 cut(s) 96, 145, 598, 739, 1234
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 5 cut(s) 93, 142, 595, 736, 1231
BstMWI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 906, 1004
BstNI CCWGG 2 cut(s) 299, 420
BstNSI RCATGY 2 cut(s) 1167, 1272
BstSCI CCNGG 3 cut(s) 297, 418, 1113
BstV1I GCAGC 2 cut(s) 23, 235
BstV2I GAAGAC 2 cut(s) 817, 1060
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
BsuRI GGCC 1 cut(s) 707
BtgZI GCGATG 1 cut(s) 1005
BtrI CACGTC 1 cut(s) 1171
BtsCI GGATG 6 cut(s) 114, 307, 673, 773, 856, 1066
BtsIMutI CAGTG 3 cut(s) 199, 406, 580
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 2 cut(s) 331, 1270
CaiI CAGNNNCTG 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 921
Cfr13I GGNCC 2 cut(s) 185, 340
Csp6I GTAC 5 cut(s) 614, 1085, 1152, 1161, 1182
CspAI ACCGGT 1 cut(s) 921
CviAII CATG 9 cut(s) 54, 89, 218, 443, 722, 1164, 1185, 1246, 1269
CviQI GTAC 5 cut(s) 614, 1085, 1152, 1161, 1182
DdeI CTNAG 5 cut(s) 259, 462, 910, 952, 1072
DpnI GATC 5 cut(s) 95, 144, 597, 738, 1233
DpnII GATC 5 cut(s) 93, 142, 595, 736, 1231
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 30
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 2 cut(s) 185, 340
Eco57I CTGAAG 2 cut(s) 642, 1090
EcoRII CCWGG 2 cut(s) 297, 418
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 1270
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 9 cut(s) 57, 92, 221, 446, 725, 1167, 1188, 1249, 1272
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FatI CATG 9 cut(s) 53, 88, 217, 442, 721, 1163, 1184, 1245, 1268
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 3 cut(s) 12, 15, 249
FokI GGATG 6 cut(s) 121, 294, 660, 780, 863, 1073
Fsp4HI GCNGC 3 cut(s) 12, 15, 249
FspBI CTAG 5 cut(s) 282, 560, 605, 1052, 1199
GluI GCNGC 3 cut(s) 12, 15, 249
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 1 cut(s) 707
HapII CCGG 3 cut(s) 727, 922, 1114
Hin1II CATG 9 cut(s) 57, 92, 221, 446, 725, 1167, 1188, 1249, 1272
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HindIII AAGCTT 2 cut(s) 624, 1005
HinfI GANTC 6 cut(s) 172, 263, 275, 549, 670, 1110
HpaII CCGG 3 cut(s) 727, 922, 1114
HphI GGTGA 2 cut(s) 349, 731
Hpy166II GTNNAC 1 cut(s) 1045
Hpy188I TCNGA 8 cut(s) 262, 344, 436, 622, 719, 850, 1081, 1109
Hpy188III TCNNGA 5 cut(s) 272, 314, 560, 605, 638
Hpy8I GTNNAC 1 cut(s) 1045
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 2 cut(s) 59, 1114
HpyCH4III ACNGT 6 cut(s) 385, 401, 575, 966, 1049, 1160
HpyCH4IV ACGT 1 cut(s) 1170
HpyCH4V TGCA 9 cut(s) 11, 168, 333, 689, 782, 1014, 1196, 1268, 1272
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 906, 1004
HpyF3I CTNAG 5 cut(s) 259, 462, 910, 952, 1072
HpySE526I ACGT 1 cut(s) 1170
Hsp92II CATG 9 cut(s) 57, 92, 221, 446, 725, 1167, 1188, 1249, 1272
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 5 cut(s) 93, 142, 595, 736, 1231
LmnI GCTCC 1 cut(s) 274
Lsp1109I GCAGC 2 cut(s) 23, 235
LweI GCATC 3 cut(s) 646, 758, 977
MaeI CTAG 5 cut(s) 282, 560, 605, 1052, 1199
MaeII ACGT 1 cut(s) 1170
MaeIII GTNAC 3 cut(s) 575, 833, 890
MalI GATC 5 cut(s) 95, 144, 597, 738, 1233
MboI GATC 5 cut(s) 93, 142, 595, 736, 1231
MboII GAAGA 9 cut(s) 12, 17, 144, 270, 436, 546, 806, 822, 1060
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 1 cut(s) 93
MluCI AATT 6 cut(s) 6, 194, 450, 797, 904, 1273
MlyI GAGTC 1 cut(s) 1119
MmeI TCCRAC 3 cut(s) 86, 163, 906
Mph1103I ATGCAT 1 cut(s) 1270
MseI TTAA 5 cut(s) 698, 1002, 1133, 1143, 1205
MslI CAYNNNNRTG 1 cut(s) 528
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 3 cut(s) 727, 922, 1114
MspR9I CCNGG 3 cut(s) 299, 420, 1115
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 2 cut(s) 299, 420
MwoI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 906, 1004
NciI CCSGG 1 cut(s) 1115
NdeII GATC 5 cut(s) 93, 142, 595, 736, 1231
NlaIII CATG 9 cut(s) 57, 92, 221, 446, 725, 1167, 1188, 1249, 1272
NmuCI GTSAC 3 cut(s) 575, 833, 890
NsiI ATGCAT 1 cut(s) 1270
NspI RCATGY 2 cut(s) 1167, 1272
PaeI GCATGC 1 cut(s) 1272
PasI CCCWGGG 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 5 cut(s) 172, 263, 275, 549, 670
PflFI GACNNNGTC 1 cut(s) 219
PflMI CCANNNNNTGG 1 cut(s) 749
PinAI ACCGGT 1 cut(s) 921
PkrI GCNGC 3 cut(s) 13, 16, 250
PleI GAGTC 1 cut(s) 1118
PpsI GAGTC 1 cut(s) 1118
Psp6I CCWGG 2 cut(s) 297, 418
PspGI CCWGG 2 cut(s) 297, 418
PspPI GGNCC 2 cut(s) 185, 340
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 93
PsyI GACNNNGTC 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 5 cut(s) 615, 1086, 1153, 1162, 1183
RsaNI GTAC 5 cut(s) 614, 1085, 1152, 1161, 1182
RseI CAYNNNNRTG 1 cut(s) 528
SaqAI TTAA 5 cut(s) 698, 1002, 1133, 1143, 1205
SatI GCNGC 3 cut(s) 12, 15, 249
Sau3AI GATC 5 cut(s) 93, 142, 595, 736, 1231
Sau96I GGNCC 2 cut(s) 185, 340
SchI GAGTC 1 cut(s) 1119
ScrFI CCNGG 3 cut(s) 299, 420, 1115
SfaNI GCATC 3 cut(s) 646, 758, 977
SinI GGWCC 2 cut(s) 185, 340
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 2 cut(s) 97, 314
SmoI CTYRAG 2 cut(s) 97, 314
SphI GCATGC 1 cut(s) 1272
Sse9I AATT 6 cut(s) 6, 194, 450, 797, 904, 1273
SsiI CCGC 1 cut(s) 251
SspMI CTAG 5 cut(s) 282, 560, 605, 1052, 1199
StyD4I CCNGG 3 cut(s) 297, 418, 1113
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 6 cut(s) 385, 401, 575, 966, 1049, 1160
TaiI ACGT 1 cut(s) 1173
TaqI TCGA 4 cut(s) 80, 130, 387, 480
TaqII GACCGA 1 cut(s) 909
TasI AATT 6 cut(s) 6, 194, 450, 797, 904, 1273
TatI WGTACW 4 cut(s) 613, 1084, 1160, 1181
TfiI GAWTC 5 cut(s) 172, 263, 275, 549, 670
Tru1I TTAA 5 cut(s) 698, 1002, 1133, 1143, 1205
Tru9I TTAA 5 cut(s) 698, 1002, 1133, 1143, 1205
TscAI CASTG 3 cut(s) 206, 406, 580
TseFI GTSAC 3 cut(s) 575, 833, 890
TseI GCWGC 3 cut(s) 11, 14, 248
Tsp45I GTSAC 3 cut(s) 575, 833, 890
TspDTI ATGAA 6 cut(s) 125, 459, 662, 807, 809, 975
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 3 cut(s) 206, 406, 580
Tth111I GACNNNGTC 1 cut(s) 219
Van91I CCANNNNNTGG 1 cut(s) 749
VpaK11BI GGWCC 2 cut(s) 185, 340
XapI RAATTY 2 cut(s) 450, 797
XbaI TCTAGA 2 cut(s) 559, 604
XceI RCATGY 2 cut(s) 1167, 1272
XspI CTAG 5 cut(s) 282, 560, 605, 1052, 1199
Zsp2I ATGCAT 1 cut(s) 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.