FvH4_4g11600

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002666
Physical Location & Seq
Reverse (-)
15300110 .. 15307938
7829 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11600.t12

Sequence Viewer

Length: 1392 bp
ATGGCAATTGCAGCTGCTTCTTCTCTTCCCTTGGGTTCTTCACATTGCCATTCATGCCATACAGAAGGAGTTTGTTGTTCAACAAAACATGGGATCTCGAACAGTTGGATGAAACCTCATTTTGATGGTCGAAGATCTCCTGATCGTCTGGGTGTAAGTTTGAAGTGCAGGAATCCGTTAGTTGGACCAACACAATTCCATTGGCTGTCTATTGGACATGGTCTTTGCCTTTCCAAAGTTTTTGTTGCTGCAGATTTCTCAGATTCTGCTCCTGAATCTTCTAGCTATATGACTAACCAGGGTTATCATCCTCTTGAGGAAGTGAAAGCGTGCAAAACGGTCCGAGACACTAAACTCACCTCTGCTGAAATAGCAAGAACAACAGTCGAGGCTAACGACAATGCTTTGCTGGTTTTCCCTGGGAAGATACACTCTGAACCGCATGAACAAATTTCTTGGGCTGAGTTTCAATATGTTATCGACGATTATGGAGATTTATATTTTGAACTGTTTGATGATGCAAACATATTAGAAGATCCTACTGCAAGTAACCCTGTGAATGCTTTGTTTGGAATGGATATCCCTGCACACAACAATGGAAGAATAACTGGAGGATTCAGCATTCTAGATGACTACAACAGTGATGATATGCCTTTTGACGATGATTATTTAGAGGTTGTGGAACCTGAAGCTTTTGATGTTCTAGACTGGGAGATACCTGATGCTTCTACCGTGATTCATCCCATTTACTTTGCAAAGTGCTTGACAAAGGCCATCAATATCAGACATGACAGGAAAATGGATCACCCATCAAATGGTGTTTCTATTTTAGGATGTCTCATACCTGCTTTTGCTGATGAAGAATTTTATGTAAGAAGACTATTTCATCATGAGGATAGTGACTATGACTCGGATGAGAAAGATGGTAAAGGAGTGAGCATTAGTTCCAAAAGTGACCGAAGCAAGACTCGCTCAACGCTCTACCGGTTGGAGATAATGAGAATAGAGCTTTTTTCAGTATATGGTGTTCAGTCCGCAATTAGCCTGCAAGATTTTCAAGATGCGGAACCTGATTTTCTTATAAACTCTATCTCGGATATCGTAGAACGCTTCAACGAAAGAGGTATTAGATGCGATGTTGCTCTTAAAGCTTTATGCAAAAGAAAAGGACTTCAAGTTGAGGGAGCTCACTTGATTGGGGTTGACAGTCTAGGAATGGATGTTAGAGTTTTCTCTGGGTCAGAAGTACAAACTCATCGTTTTCCCTTCAGAGTCCGGGCTAAGTCTGAACTTGTAGCTGAAAAACAGATTGAGCAACTTCTTTTCCCACGCTCTCGCCGTAAAAAGTTGAGAAGTCAAGGAAATAATTTTCAAGGTGTAGAGTCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

464

Amino Acids

51.94

Weight (kDa)

5.34

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1082
Acc36I ACCTGC 1 cut(s) 853
AccB7I CCANNNNNTGG 1 cut(s) 815
AciI CCGC 3 cut(s) 440, 1035, 1064
AclWI GGATC 3 cut(s) 101, 530, 810
AcsI RAATTY 2 cut(s) 450, 863
AcuI CTGAAG 2 cut(s) 708, 1252
AfaI GTAC 1 cut(s) 1248
AfiI CCNNNNNNNGG 2 cut(s) 182, 815
AgeI ACCGGT 1 cut(s) 984
AgsI TTSAA 8 cut(s) 81, 163, 470, 506, 1058, 1114, 1175, 1373
AjnI CCWGG 2 cut(s) 297, 418
AluBI AGCT 7 cut(s) 14, 285, 692, 1009, 1151, 1187, 1298
AluI AGCT 7 cut(s) 14, 285, 692, 1009, 1151, 1187, 1298
Alw21I GWGCWC 1 cut(s) 1189
Alw26I GTCTC 2 cut(s) 339, 842
AlwI GGATC 3 cut(s) 101, 530, 810
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 1 cut(s) 771
ApeKI GCWGC 3 cut(s) 11, 14, 248
ApoI RAATTY 2 cut(s) 450, 863
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AsiGI ACCGGT 1 cut(s) 984
AspS9I GGNCC 2 cut(s) 185, 340
AsuC2I CCSGG 1 cut(s) 1277
AsuHPI GGTGA 2 cut(s) 349, 797
AvaII GGWCC 2 cut(s) 185, 340
BanII GRGCYC 1 cut(s) 1189
BbsI GAAGAC 1 cut(s) 883
Bbv12I GWGCWC 1 cut(s) 1189
BbvI GCAGC 2 cut(s) 23, 235
BccI CCATC 4 cut(s) 119, 782, 817, 917
BceAI ACGGC 1 cut(s) 1323
BciT130I CCWGG 2 cut(s) 299, 420
BcnI CCSGG 1 cut(s) 1277
BcoDI GTCTC 2 cut(s) 339, 842
BfaI CTAG 4 cut(s) 282, 626, 704, 1211
BfmI CTRYAG 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 853
BglII AGATCT 1 cut(s) 134
BisI GCNGC 3 cut(s) 12, 15, 249
BlsI GCNGC 3 cut(s) 13, 16, 250
Bme1390I CCNGG 3 cut(s) 299, 420, 1277
Bme18I GGWCC 2 cut(s) 185, 340
BmgT120I GGNCC 2 cut(s) 185, 340
BmiI GGNNCC 2 cut(s) 684, 1068
BmrFI CCNGG 3 cut(s) 299, 420, 1277
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 4 cut(s) 508, 712, 1051, 1121
BmuI ACTGGG 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 883
BpmI CTGGAG 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 335
BpuMI CCSGG 1 cut(s) 1277
BsaJI CCNNGG 4 cut(s) 30, 298, 418, 419
BsaWI WCCGGW 1 cut(s) 984
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 815
Bse118I RCCGGY 1 cut(s) 984
Bse1I ACTGG 2 cut(s) 613, 713
Bse3DI GCAATG 1 cut(s) 43
BseBI CCWGG 2 cut(s) 299, 420
BseDI CCNNGG 4 cut(s) 30, 298, 418, 419
BseGI GGATG 6 cut(s) 114, 307, 739, 839, 919, 1225
BseLI CCNNNNNNNGG 2 cut(s) 182, 815
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 273, 453
BseNI ACTGG 2 cut(s) 613, 713
BseXI GCAGC 2 cut(s) 23, 235
BsgI GTGCAG 2 cut(s) 187, 570
BshFI GGCC 1 cut(s) 773
BshTI ACCGGT 1 cut(s) 984
BsiHKAI GWGCWC 1 cut(s) 1189
BsiSI CCGG 2 cut(s) 985, 1276
BslI CCNNNNNNNGG 2 cut(s) 182, 815
BsmAI GTCTC 2 cut(s) 339, 842
BsmI GAATGC 2 cut(s) 565, 621
BsnI GGCC 1 cut(s) 773
Bsp1286I GDGCHC 1 cut(s) 1189
Bsp143I GATC 5 cut(s) 93, 134, 142, 535, 802
BspACI CCGC 3 cut(s) 440, 1035, 1064
BspANI GGCC 1 cut(s) 773
BspCNI CTCAG 2 cut(s) 272, 454
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 2 cut(s) 684, 1068
BspMAI CTGCAG 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 853
BspPI GGATC 3 cut(s) 101, 530, 810
BsrDI GCAATG 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 984
BsrI ACTGG 2 cut(s) 613, 713
BssAI RCCGGY 1 cut(s) 984
BssECI CCNNGG 4 cut(s) 30, 298, 418, 419
BssMI GATC 5 cut(s) 93, 134, 142, 535, 802
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 299, 420
Bst4CI ACNGT 7 cut(s) 104, 340, 385, 510, 641, 733, 1208
Bst6I CTCTTC 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 331, 1046
BstDEI CTNAG 3 cut(s) 259, 462, 1281
BstF5I GGATG 6 cut(s) 114, 307, 739, 839, 919, 1225
BstKTI GATC 5 cut(s) 96, 137, 145, 538, 805
BstMAI GTCTC 2 cut(s) 339, 842
BstMBI GATC 5 cut(s) 93, 134, 142, 535, 802
BstMWI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 969, 1148
BstNI CCWGG 2 cut(s) 299, 420
BstSCI CCNGG 3 cut(s) 297, 418, 1275
BstSFI CTRYAG 1 cut(s) 249
BstV1I GCAGC 2 cut(s) 23, 235
BstV2I GAAGAC 1 cut(s) 883
BstX2I RGATCY 3 cut(s) 93, 134, 535
BstYI RGATCY 3 cut(s) 93, 134, 535
BsuRI GGCC 1 cut(s) 773
BtgZI GCGATG 1 cut(s) 1149
BtsCI GGATG 6 cut(s) 114, 307, 739, 839, 919, 1225
BtsIMutI CAGTG 1 cut(s) 646
BveI ACCTGC 1 cut(s) 853
Cac8I GCNNGC 2 cut(s) 331, 1046
CaiI CAGNNNCTG 1 cut(s) 266
CciI TCATGA 1 cut(s) 889
Cfr10I RCCGGY 1 cut(s) 984
Cfr13I GGNCC 2 cut(s) 185, 340
CpoI CGGWCCG 1 cut(s) 340
Csp6I GTAC 1 cut(s) 1247
CspAI ACCGGT 1 cut(s) 984
CspI CGGWCCG 1 cut(s) 340
CviAII CATG 6 cut(s) 54, 89, 218, 443, 788, 890
CviQI GTAC 1 cut(s) 1247
DdeI CTNAG 3 cut(s) 259, 462, 1281
DpnI GATC 5 cut(s) 95, 136, 144, 537, 804
DpnII GATC 5 cut(s) 93, 134, 142, 535, 802
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 1187
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 1189
Eco32I GATATC 2 cut(s) 580, 1099
Eco47I GGWCC 2 cut(s) 185, 340
Eco53kI GAGCTC 1 cut(s) 1187
Eco57I CTGAAG 2 cut(s) 708, 1252
EcoICRI GAGCTC 1 cut(s) 1187
EcoRII CCWGG 2 cut(s) 297, 418
EcoRV GATATC 2 cut(s) 580, 1099
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 1189
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 6 cut(s) 57, 92, 221, 446, 791, 893
FatI CATG 6 cut(s) 53, 88, 217, 442, 787, 889
Fnu4HI GCNGC 3 cut(s) 12, 15, 249
FokI GGATG 6 cut(s) 121, 294, 726, 846, 926, 1232
FriOI GRGCYC 1 cut(s) 1189
Fsp4HI GCNGC 3 cut(s) 12, 15, 249
FspBI CTAG 4 cut(s) 282, 626, 704, 1211
GluI GCNGC 3 cut(s) 12, 15, 249
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 1 cut(s) 773
HapII CCGG 2 cut(s) 985, 1276
Hin1II CATG 6 cut(s) 57, 92, 221, 446, 791, 893
HincII GTYRAC 1 cut(s) 1204
HindII GTYRAC 1 cut(s) 1204
HindIII AAGCTT 2 cut(s) 690, 1149
HinfI GANTC 9 cut(s) 172, 263, 275, 615, 736, 908, 967, 1272, 1382
HpaII CCGG 2 cut(s) 985, 1276
HphI GGTGA 2 cut(s) 349, 797
Hpy166II GTNNAC 1 cut(s) 1204
Hpy188I TCNGA 9 cut(s) 262, 344, 436, 785, 913, 1096, 1243, 1271, 1288
Hpy188III TCNNGA 8 cut(s) 97, 140, 272, 314, 626, 704, 890, 1058
Hpy8I GTNNAC 1 cut(s) 1204
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 2 cut(s) 59, 1276
HpyCH4III ACNGT 7 cut(s) 104, 340, 385, 510, 641, 733, 1208
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 969, 1148
HpyF3I CTNAG 3 cut(s) 259, 462, 1281
Hsp92II CATG 6 cut(s) 57, 92, 221, 446, 791, 893
Kzo9I GATC 5 cut(s) 93, 134, 142, 535, 802
LmnI GCTCC 2 cut(s) 274, 1184
Lsp1109I GCAGC 2 cut(s) 23, 235
LweI GCATC 4 cut(s) 508, 712, 1051, 1121
MaeI CTAG 4 cut(s) 282, 626, 704, 1211
MaeIII GTNAC 3 cut(s) 548, 899, 953
MalI GATC 5 cut(s) 95, 136, 144, 537, 804
MboI GATC 5 cut(s) 93, 134, 142, 535, 802
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 3 cut(s) 93, 134, 535
MhlI GDGCHC 1 cut(s) 1189
MluCI AATT 6 cut(s) 6, 194, 450, 863, 1038, 1366
MlyI GAGTC 4 cut(s) 902, 961, 1281, 1391
MmeI TCCRAC 3 cut(s) 86, 163, 969
MseI TTAA 1 cut(s) 1146
MslI CAYNNNNRTG 2 cut(s) 123, 594
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 2 cut(s) 985, 1276
MspR9I CCNGG 3 cut(s) 299, 420, 1277
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 2 cut(s) 565, 621
MvaI CCWGG 2 cut(s) 299, 420
MwoI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 969, 1148
NciI CCSGG 1 cut(s) 1277
NdeII GATC 5 cut(s) 93, 134, 142, 535, 802
NlaIII CATG 6 cut(s) 57, 92, 221, 446, 791, 893
NlaIV GGNNCC 2 cut(s) 684, 1068
NmuCI GTSAC 2 cut(s) 899, 953
PagI TCATGA 1 cut(s) 889
PasI CCCWGGG 1 cut(s) 419
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 1336
PctI GAATGC 2 cut(s) 565, 621
PfeI GAWTC 5 cut(s) 172, 263, 275, 615, 736
PflFI GACNNNGTC 1 cut(s) 219
PflMI CCANNNNNTGG 1 cut(s) 815
PinAI ACCGGT 1 cut(s) 984
PkrI GCNGC 3 cut(s) 13, 16, 250
PleI GAGTC 4 cut(s) 902, 961, 1280, 1390
PpsI GAGTC 4 cut(s) 902, 961, 1280, 1390
PsiI TTATAA 1 cut(s) 1082
Psp124BI GAGCTC 1 cut(s) 1189
Psp6I CCWGG 2 cut(s) 297, 418
PspGI CCWGG 2 cut(s) 297, 418
PspN4I GGNNCC 2 cut(s) 684, 1068
PspPI GGNCC 2 cut(s) 185, 340
PsrI GAACNNNNNNTAC 2 cut(s) 1011, 1043
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 3 cut(s) 93, 134, 535
PsyI GACNNNGTC 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 1 cut(s) 1248
RsaNI GTAC 1 cut(s) 1247
RseI CAYNNNNRTG 2 cut(s) 123, 594
Rsr2I CGGWCCG 1 cut(s) 340
RsrII CGGWCCG 1 cut(s) 340
SacI GAGCTC 1 cut(s) 1189
SaqAI TTAA 1 cut(s) 1146
SatI GCNGC 3 cut(s) 12, 15, 249
Sau3AI GATC 5 cut(s) 93, 134, 142, 535, 802
Sau96I GGNCC 2 cut(s) 185, 340
SchI GAGTC 4 cut(s) 902, 961, 1281, 1391
ScrFI CCNGG 3 cut(s) 299, 420, 1277
SduI GDGCHC 1 cut(s) 1189
SfaNI GCATC 4 cut(s) 508, 712, 1051, 1121
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 2 cut(s) 185, 340
SmiMI CAYNNNNRTG 2 cut(s) 123, 594
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 6 cut(s) 6, 194, 450, 863, 1038, 1366
SsiI CCGC 3 cut(s) 440, 1035, 1064
SspMI CTAG 4 cut(s) 282, 626, 704, 1211
SstI GAGCTC 1 cut(s) 1189
StyD4I CCNGG 3 cut(s) 297, 418, 1275
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 7 cut(s) 104, 340, 385, 510, 641, 733, 1208
TaqI TCGA 4 cut(s) 98, 130, 387, 480
TaqII GACCGA 1 cut(s) 972
TasI AATT 6 cut(s) 6, 194, 450, 863, 1038, 1366
TatI WGTACW 1 cut(s) 1246
TfiI GAWTC 5 cut(s) 172, 263, 275, 615, 736
Tru1I TTAA 1 cut(s) 1146
Tru9I TTAA 1 cut(s) 1146
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 2 cut(s) 899, 953
TseI GCWGC 3 cut(s) 11, 14, 248
Tsp45I GTSAC 2 cut(s) 899, 953
TspDTI ATGAA 6 cut(s) 42, 125, 459, 728, 873, 875
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 646
Tth111I GACNNNGTC 1 cut(s) 219
Van91I CCANNNNNTGG 1 cut(s) 815
VpaK11BI GGWCC 2 cut(s) 185, 340
XapI RAATTY 2 cut(s) 450, 863
XbaI TCTAGA 2 cut(s) 625, 703
XspI CTAG 4 cut(s) 282, 626, 704, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.