Rh4AG151100

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002666
Physical Location & Seq
Reverse (-)
38276862 .. 38283784
6923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG151100.1

Sequence Viewer

Length: 1395 bp
ATGGCAATTGCAGCTGCTTCTTCTCTTCCCTTGGGTCATTCGCATTGCCATTCATGCCACACAGAAGGAGTTTGTTGTTCGACAAGACGTGGGATCTCAAGCAGTTGGATGAAACCTCCTTTTGATGGTCGAAGATACCCTGATCCTCTGGGTGTAAGTTTGAAGTGCAGGAATCCGTTAGTTGGACCAACACAATTCCACTGGCTGTCTATTGGACATGGTCTTTGCCTTTCCAAAGTTTCGGTTGCTGCGGATTTCTCGGATTCTGCTCCTGAATCTTCTAGTTATATGACTAACCAGGGTTATCATCCTCTTGAGGAGTTGAAAGCGTGCAAAATAGTCCGAGACACTAAACTCACCTTTGCTGAAATAGCAAGAACAACAGTCGAGGCTAACGACAGTGCTTTGCTGGTTTTCCCTGGGAAGATACACTCTGAACCACATGAACAAATTTCTTGGGCTGAGTTTCAATATGTTATCGACGATTATGGAGATTTATATTTTGAACTGTTTGATGATGCGAACATATTAGAAGATCCTACAGCAAGTAACCCTGTGAATGCTTTGTTTGGAATGGATATCCCTGTACACAACAATGGAAGAATAACTGGAGGATTCAGCATTCTAGATGACCAGAACAGTGACGATATTCCTTTTGATGATGATTATCTAGAGGTTGTAGAATCTGAAGCTTTTGATGTTCTAGACTGGGAGATACCTGATGCTTCTACCTTGATTCATCCCATTTATTTTGCAAAGTGCTTAACAAAGGCCATCAATATCAGACATGACAGAAAAATGGATCACCCATTAAATGGTGTTTCTATTTTAGGATGCCTCATACCTGCTTTTGCTGATGAAGAATTTTATGTAAGAAGACTATTTCATTATGAGGATAGCGACTATTACAACTCGGATGAGAAAGGTGGAAAAGGAGTGAGCATTAGTTCCAAAAGTGACCGAAGCAAGAATTGCTCAACACTCTACCGGTTGGAGATAATGAGAATAGAGTTGTTCTCGGTATATGGTGTTCAGTCCACAATTAACCTACAAGATTTTCAAGATGCGGAACCTGATTTTCTTATAAACTCTATCTCGGATATTGTAGAACGGTTCAACGAAAGAGGTATTAGATGCGATGTTGCTCTTAAAGCTTTATGCAAAAGAAAAGGACTTCAAGTTGAGGGAGCTCACTTGATCGGGGTTGACAGTCTAGGAATGGATGTTAGAGTCTTCTCAGGGTCAGAAGTACAAACTCATCGTTTTCCCTTCAGAGTCCGGGCCAAGTCTGAAGTTGCAGCTGAAAAACAGATTGAGCAACTTTTGTTCCCACGCTCTCGCCGTAAAAAGTTGAGAAGTCAAGGAAATAATTTTCAGGGTGTGGAGTCGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

464

Amino Acids

52.1

Weight (kDa)

5.29

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1085
Acc36I ACCTGC 1 cut(s) 853
AccB7I CCANNNNNTGG 1 cut(s) 815
AciI CCGC 2 cut(s) 251, 1067
AclWI GGATC 4 cut(s) 101, 137, 530, 810
AcsI RAATTY 2 cut(s) 450, 863
AcuI CTGAAG 3 cut(s) 708, 1255, 1311
AfaI GTAC 3 cut(s) 588, 1251, 1391
AfiI CCNNNNNNNGG 3 cut(s) 125, 182, 815
AgeI ACCGGT 1 cut(s) 987
AgsI TTSAA 7 cut(s) 163, 325, 470, 506, 1061, 1117, 1178
AjiI CACGTC 1 cut(s) 89
AjnI CCWGG 2 cut(s) 297, 418
AluBI AGCT 5 cut(s) 14, 692, 1154, 1190, 1301
AluI AGCT 5 cut(s) 14, 692, 1154, 1190, 1301
Alw21I GWGCWC 1 cut(s) 1192
Alw26I GTCTC 1 cut(s) 339
AlwI GGATC 4 cut(s) 101, 137, 530, 810
AoxI GGCC 2 cut(s) 771, 1281
ApeKI GCWGC 4 cut(s) 11, 14, 248, 1298
ApoI RAATTY 2 cut(s) 450, 863
ArsI GACNNNNNNTTYG 2 cut(s) 207, 239
AsiGI ACCGGT 1 cut(s) 987
AspS9I GGNCC 2 cut(s) 185, 1281
AsuC2I CCSGG 1 cut(s) 1280
AsuHPI GGTGA 2 cut(s) 349, 797
AvaII GGWCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 1192
BbsI GAAGAC 2 cut(s) 883, 1225
Bbv12I GWGCWC 1 cut(s) 1192
BbvI GCAGC 3 cut(s) 23, 235, 1310
BccI CCATC 2 cut(s) 119, 782
BceAI ACGGC 1 cut(s) 1326
BciT130I CCWGG 2 cut(s) 299, 420
BcnI CCSGG 1 cut(s) 1280
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 5 cut(s) 282, 626, 671, 704, 1214
BfmI CTRYAG 1 cut(s) 540
BfuAI ACCTGC 1 cut(s) 853
BisI GCNGC 4 cut(s) 12, 15, 249, 1299
BlsI GCNGC 4 cut(s) 13, 16, 250, 1300
Bme1390I CCNGG 3 cut(s) 299, 420, 1280
Bme18I GGWCC 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 185, 1281
BmiI GGNNCC 1 cut(s) 1071
BmrFI CCNGG 3 cut(s) 299, 420, 1280
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 5 cut(s) 508, 712, 824, 1054, 1124
BmuI ACTGGG 1 cut(s) 718
BpiI GAAGAC 2 cut(s) 883, 1225
BplI GAGNNNNNCTC 2 cut(s) 1001, 1033
BpmI CTGGAG 1 cut(s) 630
BpuEI CTTGAG 2 cut(s) 82, 335
BpuMI CCSGG 1 cut(s) 1280
BsaBI GATNNNNATC 1 cut(s) 666
BsaJI CCNNGG 4 cut(s) 30, 298, 418, 419
BsaWI WCCGGW 1 cut(s) 987
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 182, 815
Bse118I RCCGGY 1 cut(s) 987
Bse1I ACTGG 3 cut(s) 206, 613, 713
Bse3DI GCAATG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 2 cut(s) 299, 420
BseDI CCNNGG 4 cut(s) 30, 298, 418, 419
BseGI GGATG 6 cut(s) 114, 307, 739, 839, 922, 1228
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 3 cut(s) 125, 182, 815
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 453, 1251
BseNI ACTGG 3 cut(s) 206, 613, 713
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 3 cut(s) 23, 235, 1310
BsgI GTGCAG 1 cut(s) 187
BshFI GGCC 2 cut(s) 773, 1283
BshTI ACCGGT 1 cut(s) 987
BsiHKAI GWGCWC 1 cut(s) 1192
BsiSI CCGG 2 cut(s) 988, 1279
BslI CCNNNNNNNGG 3 cut(s) 125, 182, 815
BsmAI GTCTC 1 cut(s) 339
BsmI GAATGC 2 cut(s) 565, 621
BsnI GGCC 2 cut(s) 773, 1283
Bsp1286I GDGCHC 1 cut(s) 1192
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 5 cut(s) 93, 142, 535, 802, 1197
BspACI CCGC 2 cut(s) 251, 1067
BspANI GGCC 2 cut(s) 773, 1283
BspCNI CTCAG 2 cut(s) 454, 1250
BspLI GGNNCC 1 cut(s) 1071
BspMI ACCTGC 1 cut(s) 853
BspPI GGATC 4 cut(s) 101, 137, 530, 810
BsrDI GCAATG 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 987
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 3 cut(s) 206, 613, 713
BssAI RCCGGY 1 cut(s) 987
BssECI CCNNGG 4 cut(s) 30, 298, 418, 419
BssMI GATC 5 cut(s) 93, 142, 535, 802, 1197
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 299, 420
Bst4CI ACNGT 6 cut(s) 385, 401, 510, 641, 1113, 1211
Bst6I CTCTTC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 972
BstAUI TGTACA 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 2 cut(s) 462, 1237
BstF5I GGATG 6 cut(s) 114, 307, 739, 839, 922, 1228
BstKTI GATC 5 cut(s) 96, 145, 538, 805, 1200
BstMAI GTCTC 1 cut(s) 339
BstMBI GATC 5 cut(s) 93, 142, 535, 802, 1197
BstMWI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 972, 1151
BstNI CCWGG 2 cut(s) 299, 420
BstSCI CCNGG 3 cut(s) 297, 418, 1278
BstSFI CTRYAG 1 cut(s) 540
BstV1I GCAGC 3 cut(s) 23, 235, 1310
BstV2I GAAGAC 2 cut(s) 883, 1225
BstX2I RGATCY 2 cut(s) 93, 535
BstYI RGATCY 2 cut(s) 93, 535
BsuRI GGCC 2 cut(s) 773, 1283
BtgZI GCGATG 1 cut(s) 1152
BtrI CACGTC 1 cut(s) 89
BtsCI GGATG 6 cut(s) 114, 307, 739, 839, 922, 1228
BtsIMutI CAGTG 3 cut(s) 199, 406, 646
BveI ACCTGC 1 cut(s) 853
Cac8I GCNNGC 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 987
Cfr13I GGNCC 2 cut(s) 185, 1281
Csp6I GTAC 3 cut(s) 587, 1250, 1390
CspAI ACCGGT 1 cut(s) 987
CviAII CATG 4 cut(s) 54, 218, 443, 788
CviQI GTAC 3 cut(s) 587, 1250, 1390
DdeI CTNAG 2 cut(s) 462, 1237
DpnI GATC 5 cut(s) 95, 144, 537, 804, 1199
DpnII GATC 5 cut(s) 93, 142, 535, 802, 1197
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 1190
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 1192
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 1190
Eco57I CTGAAG 3 cut(s) 708, 1255, 1311
EcoICRI GAGCTC 1 cut(s) 1190
EcoRII CCWGG 2 cut(s) 297, 418
EcoRV GATATC 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 1192
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 4 cut(s) 57, 221, 446, 791
FatI CATG 4 cut(s) 53, 217, 442, 787
Fnu4HI GCNGC 4 cut(s) 12, 15, 249, 1299
FokI GGATG 6 cut(s) 121, 294, 726, 846, 929, 1235
FriOI GRGCYC 1 cut(s) 1192
Fsp4HI GCNGC 4 cut(s) 12, 15, 249, 1299
FspBI CTAG 5 cut(s) 282, 626, 671, 704, 1214
GluI GCNGC 4 cut(s) 12, 15, 249, 1299
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 2 cut(s) 773, 1283
HapII CCGG 2 cut(s) 988, 1279
Hin1II CATG 4 cut(s) 57, 221, 446, 791
HincII GTYRAC 1 cut(s) 1207
HindII GTYRAC 1 cut(s) 1207
HindIII AAGCTT 2 cut(s) 690, 1152
HinfI GANTC 9 cut(s) 172, 263, 275, 615, 683, 736, 1230, 1275, 1385
HpaII CCGG 2 cut(s) 988, 1279
HphI GGTGA 2 cut(s) 349, 797
Hpy166II GTNNAC 3 cut(s) 589, 1038, 1207
Hpy188III TCNNGA 6 cut(s) 272, 314, 626, 671, 704, 1061
Hpy8I GTNNAC 3 cut(s) 589, 1038, 1207
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 2 cut(s) 59, 1279
HpyCH4III ACNGT 6 cut(s) 385, 401, 510, 641, 1113, 1211
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 6 cut(s) 11, 168, 333, 755, 1161, 1298
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 972, 1151
HpyF3I CTNAG 2 cut(s) 462, 1237
HpySE526I ACGT 1 cut(s) 88
Hsp92II CATG 4 cut(s) 57, 221, 446, 791
Kzo9I GATC 5 cut(s) 93, 142, 535, 802, 1197
LmnI GCTCC 2 cut(s) 274, 1187
Lsp1109I GCAGC 3 cut(s) 23, 235, 1310
LweI GCATC 5 cut(s) 508, 712, 824, 1054, 1124
MaeI CTAG 5 cut(s) 282, 626, 671, 704, 1214
MaeII ACGT 1 cut(s) 88
MaeIII GTNAC 3 cut(s) 548, 641, 956
MalI GATC 5 cut(s) 95, 144, 537, 804, 1199
MboI GATC 5 cut(s) 93, 142, 535, 802, 1197
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 2 cut(s) 93, 535
MhlI GDGCHC 1 cut(s) 1192
MluCI AATT 7 cut(s) 6, 194, 450, 863, 970, 1041, 1369
MlyI GAGTC 3 cut(s) 1239, 1284, 1394
MmeI TCCRAC 3 cut(s) 86, 163, 972
MseI TTAA 4 cut(s) 764, 812, 1044, 1149
MslI CAYNNNNRTG 1 cut(s) 594
MspA1I CMGCKG 2 cut(s) 14, 1301
MspI CCGG 2 cut(s) 988, 1279
MspR9I CCNGG 3 cut(s) 299, 420, 1280
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 2 cut(s) 565, 621
MvaI CCWGG 2 cut(s) 299, 420
MwoI GCNNNNNNNGC 5 cut(s) 11, 54, 371, 972, 1151
NciI CCSGG 1 cut(s) 1280
NdeII GATC 5 cut(s) 93, 142, 535, 802, 1197
NlaIII CATG 4 cut(s) 57, 221, 446, 791
NlaIV GGNNCC 1 cut(s) 1071
NmuCI GTSAC 2 cut(s) 641, 956
PasI CCCWGGG 1 cut(s) 419
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 1339
PctI GAATGC 2 cut(s) 565, 621
PfeI GAWTC 6 cut(s) 172, 263, 275, 615, 683, 736
PflFI GACNNNGTC 1 cut(s) 219
PflMI CCANNNNNTGG 1 cut(s) 815
PinAI ACCGGT 1 cut(s) 987
PkrI GCNGC 4 cut(s) 13, 16, 250, 1300
PleI GAGTC 3 cut(s) 1238, 1283, 1393
PpsI GAGTC 3 cut(s) 1238, 1283, 1393
PsiI TTATAA 1 cut(s) 1085
Psp124BI GAGCTC 1 cut(s) 1192
Psp6I CCWGG 2 cut(s) 297, 418
PspGI CCWGG 2 cut(s) 297, 418
PspN4I GGNNCC 1 cut(s) 1071
PspPI GGNCC 2 cut(s) 185, 1281
PsrI GAACNNNNNNTAC 2 cut(s) 1014, 1046
PsuI RGATCY 2 cut(s) 93, 535
PsyI GACNNNGTC 1 cut(s) 219
PvuII CAGCTG 2 cut(s) 14, 1301
RsaI GTAC 3 cut(s) 588, 1251, 1391
RsaNI GTAC 3 cut(s) 587, 1250, 1390
RseI CAYNNNNRTG 1 cut(s) 594
SacI GAGCTC 1 cut(s) 1192
SaqAI TTAA 4 cut(s) 764, 812, 1044, 1149
SatI GCNGC 4 cut(s) 12, 15, 249, 1299
Sau3AI GATC 5 cut(s) 93, 142, 535, 802, 1197
Sau96I GGNCC 2 cut(s) 185, 1281
SchI GAGTC 3 cut(s) 1239, 1284, 1394
ScrFI CCNGG 3 cut(s) 299, 420, 1280
SduI GDGCHC 1 cut(s) 1192
SfaNI GCATC 5 cut(s) 508, 712, 824, 1054, 1124
SfcI CTRYAG 1 cut(s) 540
SinI GGWCC 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 2 cut(s) 97, 314
SmoI CTYRAG 2 cut(s) 97, 314
Sse9I AATT 7 cut(s) 6, 194, 450, 863, 970, 1041, 1369
SsiI CCGC 2 cut(s) 251, 1067
SspMI CTAG 5 cut(s) 282, 626, 671, 704, 1214
SstI GAGCTC 1 cut(s) 1192
StyD4I CCNGG 3 cut(s) 297, 418, 1278
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 6 cut(s) 385, 401, 510, 641, 1113, 1211
TaiI ACGT 1 cut(s) 91
TaqI TCGA 4 cut(s) 80, 130, 387, 480
TaqII GACCGA 1 cut(s) 975
TasI AATT 7 cut(s) 6, 194, 450, 863, 970, 1041, 1369
TatI WGTACW 2 cut(s) 586, 1249
TfiI GAWTC 6 cut(s) 172, 263, 275, 615, 683, 736
Tru1I TTAA 4 cut(s) 764, 812, 1044, 1149
Tru9I TTAA 4 cut(s) 764, 812, 1044, 1149
TscAI CASTG 3 cut(s) 206, 406, 646
TseFI GTSAC 2 cut(s) 641, 956
TseI GCWGC 4 cut(s) 11, 14, 248, 1298
Tsp45I GTSAC 2 cut(s) 641, 956
TspDTI ATGAA 6 cut(s) 42, 125, 459, 728, 873, 875
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 3 cut(s) 206, 406, 646
Tth111I GACNNNGTC 1 cut(s) 219
Van91I CCANNNNNTGG 1 cut(s) 815
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 2 cut(s) 450, 863
XbaI TCTAGA 3 cut(s) 625, 670, 703
XspI CTAG 5 cut(s) 282, 626, 671, 704, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.