pycom04g03000

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002666
Physical Location & Seq
Reverse (-)
2770429 .. 2777831
7403 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03000.9

Sequence Viewer

Length: 1353 bp
ATGGCAATTGCAGCTGCTGCTTCTCTCCCCTTCGCAGAAAGGGTTTGTAGTTCTACAACACATGGGATCTCAAGCAGTTGGATGAAACCGCCTTTCGATGGTCGAAGAGCCCTTGATCTTCCGGGCATAAGTTTTAATTGCAGGAATCCTCTATTTGGATCAACACAATTCCATTGGCTGTCTAATGGACATGACCTTTGTCATTCCAAAGTTTCTGTTGCTGCAGATTACTCAGATTCTGTTCCCGACTCCTCTAGTTATTTGACTAGCCAAGGTTATCATCCTCTGGAAGAAGTGAAAGTGTGCAAAATGGTCCGGGACACTAAACTCACTTCTGCTGAAATAGCAAGAACAACAGTAGAGGCTAACTGCAGTGCTACGGTGGTGTTCCCGGGGAGGGTACATTGTGAACCACATGAACAAATTTCCTGGGCTGAGTTTGAATATGTTATCGACGATTGCGGAGATTTATATTTTGAAATTTTTGATGATGCAAACTTATTAGAAGATCGTACAGCAAGTAGCCCTGTGAATGTTTTGTTCGGGATGGATATCCTTGCATATGATGATGCAAGAATATCTGGTGATTTCAGCATTCTAGATGGTAGCAGTAGCGATGAAATCCCTTTGGATGATTATTATTCAGAGGTTGTGGACTCTGAAGTTTCTGATGTTCTAGACTGGGGGTTGCCAGATACTTGTAGCTCAATTCATCCTATATGTTTTGCGAAGTACTTGACAAAGGCTATTGATAATGAATACAATAGGAAAATGGATCATCCATCCAATGGCGTCTCTATTTTAGGATGCCTCAGGCCTGCTTTTGCTGATGAAGAATTCTATGTAAGAAGACTGTTTAATTACGAAGATAGCGATGGATACAACTCGGACTGGAAAGATGGAAAAAGTGTGAGCCTTAGTTCCGAAAGTGATCGTAGCTCAACTCTCTACAGGTTGGAGATCATGAGAATAGAGTTGTTCTCTGTATATGGTGTTCAGTCTACAATTAGCTTGCATGATTTTCAAGATGCAGAACCTGATGTTCTAGTAAACTCTACCTCAGAAATTGTAGAACGCTTCAGTGAAAGAGGTATTAGGTGCGATGTTGCTCTTAAAGCTTTATGCAAAAGGAAAGGACTTCATGTTGAGGGAGCTCATTTAATCGGGGTTGACAGTCTTGGAATGGATGTTAGAGTCTTCTCAGGGTTGGAAGTGCAAACTCATCGTTTTCCCTTCAAAGTCCGGGCCACATCCGAAGTTGCAGCTGAAAAACAGATTCAGCAACTTTTGTTCCCACGCTCTCGCCGCAAAAAGTTGAGTGCTCATAATGTTAGGGGCGTGGAGTCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

451

Amino Acids

50.27

Weight (kDa)

4.93

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 788
AccI GTMKAC 1 cut(s) 1001
AciI CCGC 3 cut(s) 89, 462, 1306
AclWI GGATC 3 cut(s) 74, 166, 783
AcsI RAATTY 3 cut(s) 423, 480, 836
AcuI CTGAAG 2 cut(s) 681, 1063
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 3 cut(s) 402, 514, 734
AfiI CCNNNNNNNGG 4 cut(s) 98, 155, 397, 788
AgsI TTSAA 4 cut(s) 443, 479, 1025, 1237
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 7 cut(s) 14, 705, 939, 1011, 1118, 1154, 1265
AluI AGCT 7 cut(s) 14, 705, 939, 1011, 1118, 1154, 1265
Alw21I GWGCWC 2 cut(s) 1156, 1324
Alw26I GTCTC 1 cut(s) 799
AlwI GGATC 3 cut(s) 74, 166, 783
AlwNI CAGNNNCTG 3 cut(s) 17, 239, 1037
Ama87I CYCGRG 1 cut(s) 391
AoxI GGCC 2 cut(s) 815, 1245
ApeKI GCWGC 5 cut(s) 11, 14, 17, 221, 1262
ApoI RAATTY 3 cut(s) 423, 480, 836
ArsI GACNNNNNNTTYG 2 cut(s) 180, 212
AspS9I GGNCC 2 cut(s) 313, 1245
AsuC2I CCSGG 5 cut(s) 123, 317, 392, 393, 1244
AsuHPI GGTGA 1 cut(s) 596
AvaI CYCGRG 1 cut(s) 391
AvaII GGWCC 1 cut(s) 313
AxyI CCTNAGG 1 cut(s) 812
BanII GRGCYC 2 cut(s) 112, 1156
BbsI GAAGAC 2 cut(s) 856, 1189
Bbv12I GWGCWC 2 cut(s) 1156, 1324
BbvI GCAGC 4 cut(s) 4, 23, 208, 1274
BccI CCATC 6 cut(s) 92, 541, 596, 790, 869, 893
BcgI CGANNNNNNTGC 2 cut(s) 1205, 1239
BciT130I CCWGG 1 cut(s) 430
BciVI GTATCC 1 cut(s) 872
BcnI CCSGG 5 cut(s) 123, 317, 392, 393, 1244
BcoDI GTCTC 1 cut(s) 799
BfaI CTAG 5 cut(s) 255, 267, 599, 677, 1046
BfmI CTRYAG 3 cut(s) 222, 370, 949
BfuI GTATCC 1 cut(s) 872
BisI GCNGC 6 cut(s) 12, 15, 18, 222, 1263, 1306
BlsI GCNGC 6 cut(s) 13, 16, 19, 223, 1264, 1307
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 6 cut(s) 123, 317, 392, 393, 430, 1244
Bme18I GGWCC 1 cut(s) 313
BmeT110I CYCGRG 1 cut(s) 391
BmgT120I GGNCC 2 cut(s) 313, 1245
BmrFI CCNGG 6 cut(s) 123, 317, 392, 393, 430, 1244
BmrI ACTGGG 1 cut(s) 691
BmsI GCATC 4 cut(s) 481, 559, 797, 1018
BmuI ACTGGG 1 cut(s) 691
BoxI GACNNNNGTC 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 856, 1189
BplI GAGNNNNNCTC 2 cut(s) 965, 997
BpuEI CTTGAG 1 cut(s) 55
BpuMI CCSGG 5 cut(s) 123, 317, 392, 393, 1244
BsaBI GATNNNNATC 1 cut(s) 551
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 4 cut(s) 271, 391, 392, 429
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 155, 397, 788
Bse1I ACTGG 2 cut(s) 686, 896
Bse21I CCTNAGG 1 cut(s) 812
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 1 cut(s) 430
BseDI CCNNGG 4 cut(s) 271, 391, 392, 429
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 4 cut(s) 98, 155, 397, 788
BseMII CTCAG 5 cut(s) 246, 426, 826, 1074, 1215
BseNI ACTGG 2 cut(s) 686, 896
BseRI GAGGAG 1 cut(s) 241
BseXI GCAGC 4 cut(s) 4, 23, 208, 1274
BshFI GGCC 2 cut(s) 817, 1247
BsiHKAI GWGCWC 2 cut(s) 1156, 1324
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 4 cut(s) 122, 316, 392, 1243
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 4 cut(s) 98, 155, 397, 788
BsmAI GTCTC 1 cut(s) 799
BsmBI CGTCTC 1 cut(s) 799
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 594
BsnI GGCC 2 cut(s) 817, 1247
BsoBI CYCGRG 1 cut(s) 391
Bsp1286I GDGCHC 3 cut(s) 112, 1156, 1324
Bsp143I GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
BspACI CCGC 3 cut(s) 89, 462, 1306
BspANI GGCC 2 cut(s) 817, 1247
BspCNI CTCAG 5 cut(s) 245, 427, 825, 1073, 1214
BspHI TCATGA 1 cut(s) 963
BspMAI CTGCAG 2 cut(s) 226, 374
BspPI GGATC 3 cut(s) 74, 166, 783
BspQI GCTCTTC 1 cut(s) 100
BsrI ACTGG 2 cut(s) 686, 896
BssECI CCNNGG 4 cut(s) 271, 391, 392, 429
BssMI GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
BssNI GRCGYC 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 4 cut(s) 358, 382, 855, 1175
Bst6I CTCTTC 1 cut(s) 100
BstACI GRCGYC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 819, 1013
BstDEI CTNAG 6 cut(s) 232, 435, 812, 917, 1060, 1201
BstKTI GATC 7 cut(s) 69, 118, 161, 511, 778, 934, 963
BstMAI GTCTC 1 cut(s) 799
BstMBI GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
BstMWI GCNNNNNNNGC 5 cut(s) 11, 17, 344, 1115, 1305
BstNI CCWGG 1 cut(s) 430
BstPAI GACNNNNGTC 1 cut(s) 198
BstSCI CCNGG 6 cut(s) 121, 315, 390, 391, 428, 1242
BstSFI CTRYAG 3 cut(s) 222, 370, 949
BstV1I GCAGC 4 cut(s) 4, 23, 208, 1274
BstV2I GAAGAC 2 cut(s) 856, 1189
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 812
BsuI GTATCC 1 cut(s) 872
BsuRI GGCC 2 cut(s) 817, 1247
BtgZI GCGATG 3 cut(s) 630, 888, 1116
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 2 cut(s) 379, 1087
Cac8I GCNNGC 2 cut(s) 819, 1013
CaiI CAGNNNCTG 3 cut(s) 17, 239, 1037
CciI TCATGA 1 cut(s) 963
Cfr13I GGNCC 2 cut(s) 313, 1245
Cfr9I CCCGGG 1 cut(s) 391
CseI GACGC 1 cut(s) 781
Csp6I GTAC 3 cut(s) 401, 513, 733
CviAII CATG 6 cut(s) 62, 191, 416, 964, 1016, 1142
CviQI GTAC 3 cut(s) 401, 513, 733
DdeI CTNAG 6 cut(s) 232, 435, 812, 917, 1060, 1201
DpnI GATC 7 cut(s) 68, 117, 160, 510, 777, 933, 962
DpnII GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 1154
Eco130I CCWWGG 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 817
Eco24I GRGCYC 2 cut(s) 112, 1156
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 1154
Eco57I CTGAAG 2 cut(s) 681, 1063
Eco81I CCTNAGG 1 cut(s) 812
Eco88I CYCGRG 1 cut(s) 391
EcoICRI GAGCTC 1 cut(s) 1154
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 1 cut(s) 428
EcoRV GATATC 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 112, 1156
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 799
FaeI CATG 6 cut(s) 65, 194, 419, 967, 1019, 1145
FaqI GGGAC 1 cut(s) 332
FatI CATG 6 cut(s) 61, 190, 415, 963, 1015, 1141
FauNDI CATATG 1 cut(s) 562
FblI GTMKAC 1 cut(s) 1001
Fnu4HI GCNGC 6 cut(s) 12, 15, 18, 222, 1263, 1306
FriOI GRGCYC 2 cut(s) 112, 1156
Fsp4HI GCNGC 6 cut(s) 12, 15, 18, 222, 1263, 1306
FspBI CTAG 5 cut(s) 255, 267, 599, 677, 1046
GluI GCNGC 6 cut(s) 12, 15, 18, 222, 1263, 1306
HaeIII GGCC 2 cut(s) 817, 1247
HapII CCGG 4 cut(s) 122, 316, 392, 1243
HgaI GACGC 1 cut(s) 781
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 6 cut(s) 65, 194, 419, 967, 1019, 1145
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HindIII AAGCTT 1 cut(s) 1116
HinfI GANTC 7 cut(s) 145, 236, 248, 656, 1194, 1276, 1343
HpaII CCGG 4 cut(s) 122, 316, 392, 1243
HphI GGTGA 1 cut(s) 596
Hpy166II GTNNAC 5 cut(s) 410, 655, 1002, 1051, 1171
Hpy188I TCNGA 8 cut(s) 235, 646, 661, 670, 889, 925, 1063, 1255
Hpy188III TCNNGA 7 cut(s) 245, 287, 544, 599, 677, 964, 1025
Hpy8I GTNNAC 5 cut(s) 410, 655, 1002, 1051, 1171
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 2 cut(s) 40, 1243
HpyCH4III ACNGT 4 cut(s) 358, 382, 855, 1175
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 17, 344, 1115, 1305
HpyF3I CTNAG 6 cut(s) 232, 435, 812, 917, 1060, 1201
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 6 cut(s) 65, 194, 419, 967, 1019, 1145
Kzo9I GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
LguI GCTCTTC 1 cut(s) 100
LmnI GCTCC 1 cut(s) 1151
Lsp1109I GCAGC 4 cut(s) 4, 23, 208, 1274
LweI GCATC 4 cut(s) 481, 559, 797, 1018
MaeI CTAG 5 cut(s) 255, 267, 599, 677, 1046
MalI GATC 7 cut(s) 68, 117, 160, 510, 777, 933, 962
MboI GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
MboII GAAGA 8 cut(s) 110, 117, 302, 518, 845, 861, 878, 1189
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 3 cut(s) 112, 1156, 1324
MlyI GAGTC 4 cut(s) 242, 650, 1203, 1352
MmeI TCCRAC 3 cut(s) 59, 936, 1188
MseI TTAA 4 cut(s) 135, 858, 1113, 1160
MspA1I CMGCKG 2 cut(s) 14, 1265
MspI CCGG 4 cut(s) 122, 316, 392, 1243
MspR9I CCNGG 6 cut(s) 123, 317, 392, 393, 430, 1244
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 594
MvaI CCWGG 1 cut(s) 430
MwoI GCNNNNNNNGC 5 cut(s) 11, 17, 344, 1115, 1305
NciI CCSGG 5 cut(s) 123, 317, 392, 393, 1244
NdeI CATATG 1 cut(s) 562
NdeII GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
NlaIII CATG 6 cut(s) 65, 194, 419, 967, 1019, 1145
PagI TCATGA 1 cut(s) 963
PceI AGGCCT 1 cut(s) 817
PciSI GCTCTTC 1 cut(s) 100
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PctI GAATGC 1 cut(s) 594
PfeI GAWTC 3 cut(s) 145, 236, 1276
PflMI CCANNNNNTGG 1 cut(s) 788
PfoI TCCNGGA 1 cut(s) 315
PkrI GCNGC 6 cut(s) 13, 16, 19, 223, 1264, 1307
PleI GAGTC 4 cut(s) 242, 650, 1202, 1351
PpsI GAGTC 4 cut(s) 242, 650, 1202, 1351
PshAI GACNNNNGTC 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 1156
Psp6I CCWGG 1 cut(s) 428
PspGI CCWGG 1 cut(s) 428
PspPI GGNCC 2 cut(s) 313, 1245
PsrI GAACNNNNNNTAC 2 cut(s) 978, 1010
PstI CTGCAG 2 cut(s) 226, 374
PstNI CAGNNNCTG 3 cut(s) 17, 239, 1037
PsuI RGATCY 1 cut(s) 66
PvuII CAGCTG 2 cut(s) 14, 1265
RsaI GTAC 3 cut(s) 402, 514, 734
RsaNI GTAC 3 cut(s) 401, 513, 733
SacI GAGCTC 1 cut(s) 1156
SapI GCTCTTC 1 cut(s) 100
SaqAI TTAA 4 cut(s) 135, 858, 1113, 1160
SatI GCNGC 6 cut(s) 12, 15, 18, 222, 1263, 1306
Sau3AI GATC 7 cut(s) 66, 115, 158, 508, 775, 931, 960
Sau96I GGNCC 2 cut(s) 313, 1245
ScaI AGTACT 1 cut(s) 734
SchI GAGTC 4 cut(s) 242, 650, 1203, 1352
ScrFI CCNGG 6 cut(s) 123, 317, 392, 393, 430, 1244
SduI GDGCHC 3 cut(s) 112, 1156, 1324
SfaNI GCATC 4 cut(s) 481, 559, 797, 1018
SfcI CTRYAG 3 cut(s) 222, 370, 949
SinI GGWCC 1 cut(s) 313
SmaI CCCGGG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
SseBI AGGCCT 1 cut(s) 817
SsiI CCGC 3 cut(s) 89, 462, 1306
SspMI CTAG 5 cut(s) 255, 267, 599, 677, 1046
SstI GAGCTC 1 cut(s) 1156
StuI AGGCCT 1 cut(s) 817
StyD4I CCNGG 6 cut(s) 121, 315, 390, 391, 428, 1242
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 4 cut(s) 358, 382, 855, 1175
TaqI TCGA 3 cut(s) 96, 103, 453
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 1308
TfiI GAWTC 3 cut(s) 145, 236, 1276
Tru1I TTAA 4 cut(s) 135, 858, 1113, 1160
Tru9I TTAA 4 cut(s) 135, 858, 1113, 1160
TscAI CASTG 2 cut(s) 379, 1087
TseI GCWGC 5 cut(s) 11, 14, 17, 221, 1262
TspDTI ATGAA 7 cut(s) 98, 432, 633, 701, 771, 846, 1130
TspMI CCCGGG 1 cut(s) 391
TspRI CASTG 2 cut(s) 379, 1087
Van91I CCANNNNNTGG 1 cut(s) 788
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 3 cut(s) 423, 480, 836
XbaI TCTAGA 2 cut(s) 598, 676
XmaI CCCGGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 1001
XspI CTAG 5 cut(s) 255, 267, 599, 677, 1046
ZrmI AGTACT 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.