Rh4BG148500

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002666
Physical Location & Seq
Reverse (-)
26621379 .. 26628893
7515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG148500.1

Sequence Viewer

Length: 1383 bp
ATGGCAATTGCAGCTGCTTCTTCTTTTCCCTTGGGTCATTCGCATTGCCATTCATGCCACACAGAAGGAGTTTGTTGTTCGACAAGACATGGGATCTCAAGCAGTTGGATGAAACCTCCTTTTGATGGTCGAAGATACCCTGATCCTCTGGGTGTAAGGAATCCGTTAGTTGGACCAACACAGTTCCACTGGCTGTCTATTGGACATGGTCTTTGCCTTTCCAAAGTTTCGGTTGCGGCGGATTTCTCGGATTCTGCTCCTGAATCTTCTAGTTATATGACTAACCAGGGCTATCATCCTCTTGAGGAGTTGAAAGCGTGCAAAATGGTCCGAGACACTAAACTCACCTCTGCTGAAATAGCAAGAACAACAGTCGAGGCTAACGACAGTGCTTTGCTGGTTTTCCCTGGGAAGATACACTCTGAACCACATGAACAAATTTCTTGGGCTGAGTTTCAATATGTTATCGACGATTATGGAGATTTATATTTTGAACTGTTTGATGATGCGAACATATTAGAAGATCCTACAGCAAGTAACCCTGTGAATGCTTTGTTTGGAATGGATATCCCTGTACACAACAATGGAAGAATAACTGGAGGATTCAGCATTCTAGATGACCAGAACAGTGACGATATTCCTTTTGATGATGATTATCTAGAGGTTGTAGAATCTGAAGCTTTTGATGTTCTAGACTGGGAGATACCTGATGCTTCTACCTTGATTCATCCCATTTATTTTGCTAAGTGCTTAACAAAGGCCATCAATATCAGACATGACAGAAAAATGGATCACCCATCAAATGGTGTTTCTATTTTAGGATGCCTCATACCTGCTTTTGCTGATGAAGAATTTTATGTAAGAAGACTATTTCATTATGAGGATAGCGACTATTACAACTCGGATGAGAAAGGTGGAAAAGGAGTGAGCATTAGTTCCAAAAGTGACCGAAGCAAGAATTGTTCAACACTCTACCGGTTGGAGATAATGAGAATAGAGTTGTTCTCGGTATATGGTGTTCAGTCCACAATTAGCCTACAAGATTTTCAAGATGCGGAACCTGATTTTCTGATAAACTCTATCTCGGATATTGTAGAACGGTTCAACGAAAGAGGTATTAGATGCGATGTTGCTCTTAAAGCTTTATGCAAAAGAAAAGGACTTCAAGTTGAGGGAGCTCACTTGATCGGGGTTGACAGTCTAGGAATGGATGTTAGAGTCTTCTCAGGGTCAGAAGTACAAACTCATCGTTTTCCCTTCAGAGTCCGGGCCAAGTCTGAAGTTGCAGCTGAAAAACAGATTGAGCAACTTTTGTTCCCACGCTCTCGCCGTAAAAAGTTGAGAAGTCACGGAAATAATTTTCAGGGTGTGGAGTCGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

460

Amino Acids

51.6

Weight (kDa)

5.28

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 841
AccB7I CCANNNNNTGG 1 cut(s) 803
AciI CCGC 3 cut(s) 236, 239, 1055
AclWI GGATC 4 cut(s) 101, 137, 518, 798
AcsI RAATTY 2 cut(s) 438, 851
AcuI CTGAAG 3 cut(s) 696, 1243, 1299
AfaI GTAC 3 cut(s) 576, 1239, 1379
AfiI CCNNNNNNNGG 3 cut(s) 125, 170, 803
AgeI ACCGGT 1 cut(s) 975
AgsI TTSAA 7 cut(s) 313, 458, 494, 966, 1049, 1105, 1166
AjnI CCWGG 2 cut(s) 285, 406
AluBI AGCT 5 cut(s) 14, 680, 1142, 1178, 1289
AluI AGCT 5 cut(s) 14, 680, 1142, 1178, 1289
Alw21I GWGCWC 1 cut(s) 1180
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 4 cut(s) 101, 137, 518, 798
AoxI GGCC 2 cut(s) 759, 1269
ApeKI GCWGC 3 cut(s) 11, 14, 1286
ApoI RAATTY 2 cut(s) 438, 851
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AsiGI ACCGGT 1 cut(s) 975
AspS9I GGNCC 3 cut(s) 173, 328, 1269
AsuC2I CCSGG 1 cut(s) 1268
AsuHPI GGTGA 2 cut(s) 337, 785
AvaII GGWCC 2 cut(s) 173, 328
BanII GRGCYC 1 cut(s) 1180
BbsI GAAGAC 2 cut(s) 871, 1213
Bbv12I GWGCWC 1 cut(s) 1180
BbvI GCAGC 2 cut(s) 23, 1298
BccI CCATC 3 cut(s) 119, 770, 805
BceAI ACGGC 1 cut(s) 1314
BciT130I CCWGG 2 cut(s) 287, 408
BcnI CCSGG 1 cut(s) 1268
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 5 cut(s) 270, 614, 659, 692, 1202
BfmI CTRYAG 1 cut(s) 528
BfuAI ACCTGC 1 cut(s) 841
BisI GCNGC 4 cut(s) 12, 15, 237, 1287
BlsI GCNGC 4 cut(s) 13, 16, 238, 1288
Bme1390I CCNGG 3 cut(s) 287, 408, 1268
Bme18I GGWCC 2 cut(s) 173, 328
BmgT120I GGNCC 3 cut(s) 173, 328, 1269
BmiI GGNNCC 1 cut(s) 1059
BmrFI CCNGG 3 cut(s) 287, 408, 1268
BmrI ACTGGG 1 cut(s) 706
BmsI GCATC 5 cut(s) 496, 700, 812, 1042, 1112
BmuI ACTGGG 1 cut(s) 706
BpiI GAAGAC 2 cut(s) 871, 1213
BplI GAGNNNNNCTC 2 cut(s) 989, 1021
BpmI CTGGAG 1 cut(s) 618
BpuEI CTTGAG 2 cut(s) 82, 323
BpuMI CCSGG 1 cut(s) 1268
BsaBI GATNNNNATC 1 cut(s) 654
BsaJI CCNNGG 4 cut(s) 30, 286, 406, 407
BsaWI WCCGGW 1 cut(s) 975
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 170, 803
Bse118I RCCGGY 1 cut(s) 975
Bse1I ACTGG 3 cut(s) 194, 601, 701
Bse3DI GCAATG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 654
BseBI CCWGG 2 cut(s) 287, 408
BseDI CCNNGG 4 cut(s) 30, 286, 406, 407
BseGI GGATG 6 cut(s) 114, 295, 727, 827, 910, 1216
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 3 cut(s) 125, 170, 803
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 441, 1239
BseNI ACTGG 3 cut(s) 194, 601, 701
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 23, 1298
BshFI GGCC 2 cut(s) 761, 1271
BshTI ACCGGT 1 cut(s) 975
BsiHKAI GWGCWC 1 cut(s) 1180
BsiSI CCGG 2 cut(s) 976, 1267
BslI CCNNNNNNNGG 3 cut(s) 125, 170, 803
BsmAI GTCTC 1 cut(s) 327
BsmI GAATGC 2 cut(s) 553, 609
BsnI GGCC 2 cut(s) 761, 1271
Bsp1286I GDGCHC 1 cut(s) 1180
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 5 cut(s) 93, 142, 523, 790, 1185
BspACI CCGC 3 cut(s) 236, 239, 1055
BspANI GGCC 2 cut(s) 761, 1271
BspCNI CTCAG 2 cut(s) 442, 1238
BspLI GGNNCC 1 cut(s) 1059
BspMI ACCTGC 1 cut(s) 841
BspPI GGATC 4 cut(s) 101, 137, 518, 798
BsrDI GCAATG 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 975
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 3 cut(s) 194, 601, 701
BssAI RCCGGY 1 cut(s) 975
BssECI CCNNGG 4 cut(s) 30, 286, 406, 407
BssMI GATC 5 cut(s) 93, 142, 523, 790, 1185
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 287, 408
Bst4CI ACNGT 7 cut(s) 183, 373, 389, 498, 629, 1101, 1199
BstAUI TGTACA 1 cut(s) 574
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 3 cut(s) 450, 744, 1225
BstF5I GGATG 6 cut(s) 114, 295, 727, 827, 910, 1216
BstKTI GATC 5 cut(s) 96, 145, 526, 793, 1188
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 5 cut(s) 93, 142, 523, 790, 1185
BstMWI GCNNNNNNNGC 4 cut(s) 11, 54, 359, 1139
BstNI CCWGG 2 cut(s) 287, 408
BstSCI CCNGG 3 cut(s) 285, 406, 1266
BstSFI CTRYAG 1 cut(s) 528
BstV1I GCAGC 2 cut(s) 23, 1298
BstV2I GAAGAC 2 cut(s) 871, 1213
BstX2I RGATCY 2 cut(s) 93, 523
BstYI RGATCY 2 cut(s) 93, 523
BsuRI GGCC 2 cut(s) 761, 1271
BtgZI GCGATG 1 cut(s) 1140
BtsCI GGATG 6 cut(s) 114, 295, 727, 827, 910, 1216
BtsIMutI CAGTG 3 cut(s) 187, 394, 634
BveI ACCTGC 1 cut(s) 841
Cac8I GCNNGC 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 975
Cfr13I GGNCC 3 cut(s) 173, 328, 1269
Csp6I GTAC 3 cut(s) 575, 1238, 1378
CspAI ACCGGT 1 cut(s) 975
CviAII CATG 5 cut(s) 54, 89, 206, 431, 776
CviQI GTAC 3 cut(s) 575, 1238, 1378
DdeI CTNAG 3 cut(s) 450, 744, 1225
DpnI GATC 5 cut(s) 95, 144, 525, 792, 1187
DpnII GATC 5 cut(s) 93, 142, 523, 790, 1185
EciI GGCGGA 1 cut(s) 254
Ecl136II GAGCTC 1 cut(s) 1178
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 1180
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 2 cut(s) 173, 328
Eco53kI GAGCTC 1 cut(s) 1178
Eco57I CTGAAG 3 cut(s) 696, 1243, 1299
EcoICRI GAGCTC 1 cut(s) 1178
EcoRII CCWGG 2 cut(s) 285, 406
EcoRV GATATC 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 1180
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 5 cut(s) 57, 92, 209, 434, 779
FatI CATG 5 cut(s) 53, 88, 205, 430, 775
Fnu4HI GCNGC 4 cut(s) 12, 15, 237, 1287
FokI GGATG 6 cut(s) 121, 282, 714, 834, 917, 1223
FriOI GRGCYC 1 cut(s) 1180
Fsp4HI GCNGC 4 cut(s) 12, 15, 237, 1287
FspBI CTAG 5 cut(s) 270, 614, 659, 692, 1202
GluI GCNGC 4 cut(s) 12, 15, 237, 1287
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 2 cut(s) 761, 1271
HapII CCGG 2 cut(s) 976, 1267
Hin1II CATG 5 cut(s) 57, 92, 209, 434, 779
HincII GTYRAC 1 cut(s) 1195
HindII GTYRAC 1 cut(s) 1195
HindIII AAGCTT 2 cut(s) 678, 1140
HinfI GANTC 9 cut(s) 160, 251, 263, 603, 671, 724, 1218, 1263, 1373
HpaII CCGG 2 cut(s) 976, 1267
HphI GGTGA 2 cut(s) 337, 785
Hpy166II GTNNAC 3 cut(s) 577, 1026, 1195
Hpy188III TCNNGA 6 cut(s) 260, 302, 614, 659, 692, 1049
Hpy8I GTNNAC 3 cut(s) 577, 1026, 1195
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 2 cut(s) 59, 1267
HpyCH4III ACNGT 7 cut(s) 183, 373, 389, 498, 629, 1101, 1199
HpyCH4V TGCA 4 cut(s) 11, 321, 1149, 1286
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 54, 359, 1139
HpyF3I CTNAG 3 cut(s) 450, 744, 1225
Hsp92II CATG 5 cut(s) 57, 92, 209, 434, 779
Kzo9I GATC 5 cut(s) 93, 142, 523, 790, 1185
LmnI GCTCC 2 cut(s) 262, 1175
Lsp1109I GCAGC 2 cut(s) 23, 1298
LweI GCATC 5 cut(s) 496, 700, 812, 1042, 1112
MaeI CTAG 5 cut(s) 270, 614, 659, 692, 1202
MaeIII GTNAC 4 cut(s) 536, 629, 944, 1346
MalI GATC 5 cut(s) 95, 144, 525, 792, 1187
MboI GATC 5 cut(s) 93, 142, 523, 790, 1185
MboII GAAGA 9 cut(s) 12, 144, 258, 424, 533, 600, 860, 876, 1213
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 2 cut(s) 93, 523
MhlI GDGCHC 1 cut(s) 1180
MluCI AATT 6 cut(s) 6, 438, 851, 958, 1029, 1357
MlyI GAGTC 3 cut(s) 1227, 1272, 1382
MmeI TCCRAC 3 cut(s) 86, 151, 960
MseI TTAA 2 cut(s) 752, 1137
MslI CAYNNNNRTG 1 cut(s) 582
MspA1I CMGCKG 2 cut(s) 14, 1289
MspI CCGG 2 cut(s) 976, 1267
MspR9I CCNGG 3 cut(s) 287, 408, 1268
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 2 cut(s) 553, 609
MvaI CCWGG 2 cut(s) 287, 408
MwoI GCNNNNNNNGC 4 cut(s) 11, 54, 359, 1139
NciI CCSGG 1 cut(s) 1268
NdeII GATC 5 cut(s) 93, 142, 523, 790, 1185
NlaIII CATG 5 cut(s) 57, 92, 209, 434, 779
NlaIV GGNNCC 1 cut(s) 1059
NmuCI GTSAC 3 cut(s) 629, 944, 1346
PasI CCCWGGG 1 cut(s) 407
PcsI WCGNNNNNNNCGW 2 cut(s) 381, 1327
PctI GAATGC 2 cut(s) 553, 609
PfeI GAWTC 6 cut(s) 160, 251, 263, 603, 671, 724
PflFI GACNNNGTC 1 cut(s) 207
PflMI CCANNNNNTGG 1 cut(s) 803
PinAI ACCGGT 1 cut(s) 975
PkrI GCNGC 4 cut(s) 13, 16, 238, 1288
PleI GAGTC 3 cut(s) 1226, 1271, 1381
PpsI GAGTC 3 cut(s) 1226, 1271, 1381
Psp124BI GAGCTC 1 cut(s) 1180
Psp6I CCWGG 2 cut(s) 285, 406
PspGI CCWGG 2 cut(s) 285, 406
PspN4I GGNNCC 1 cut(s) 1059
PspPI GGNCC 3 cut(s) 173, 328, 1269
PsrI GAACNNNNNNTAC 2 cut(s) 1002, 1034
PsuI RGATCY 2 cut(s) 93, 523
PsyI GACNNNGTC 1 cut(s) 207
PvuII CAGCTG 2 cut(s) 14, 1289
RsaI GTAC 3 cut(s) 576, 1239, 1379
RsaNI GTAC 3 cut(s) 575, 1238, 1378
RseI CAYNNNNRTG 1 cut(s) 582
SacI GAGCTC 1 cut(s) 1180
SaqAI TTAA 2 cut(s) 752, 1137
SatI GCNGC 4 cut(s) 12, 15, 237, 1287
Sau3AI GATC 5 cut(s) 93, 142, 523, 790, 1185
Sau96I GGNCC 3 cut(s) 173, 328, 1269
SchI GAGTC 3 cut(s) 1227, 1272, 1382
ScrFI CCNGG 3 cut(s) 287, 408, 1268
SduI GDGCHC 1 cut(s) 1180
SfaNI GCATC 5 cut(s) 496, 700, 812, 1042, 1112
SfcI CTRYAG 1 cut(s) 528
SinI GGWCC 2 cut(s) 173, 328
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 97, 302
SmoI CTYRAG 2 cut(s) 97, 302
Sse9I AATT 6 cut(s) 6, 438, 851, 958, 1029, 1357
SsiI CCGC 3 cut(s) 236, 239, 1055
SspMI CTAG 5 cut(s) 270, 614, 659, 692, 1202
SstI GAGCTC 1 cut(s) 1180
StyD4I CCNGG 3 cut(s) 285, 406, 1266
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 7 cut(s) 183, 373, 389, 498, 629, 1101, 1199
TaqI TCGA 4 cut(s) 80, 130, 375, 468
TaqII GACCGA 1 cut(s) 963
TasI AATT 6 cut(s) 6, 438, 851, 958, 1029, 1357
TatI WGTACW 2 cut(s) 574, 1237
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 6 cut(s) 160, 251, 263, 603, 671, 724
Tru1I TTAA 2 cut(s) 752, 1137
Tru9I TTAA 2 cut(s) 752, 1137
TscAI CASTG 3 cut(s) 194, 394, 634
TseFI GTSAC 3 cut(s) 629, 944, 1346
TseI GCWGC 3 cut(s) 11, 14, 1286
Tsp45I GTSAC 3 cut(s) 629, 944, 1346
TspDTI ATGAA 6 cut(s) 42, 125, 447, 716, 861, 863
TspGWI ACGGA 2 cut(s) 153, 1365
TspRI CASTG 3 cut(s) 194, 394, 634
Tth111I GACNNNGTC 1 cut(s) 207
Van91I CCANNNNNTGG 1 cut(s) 803
VpaK11BI GGWCC 2 cut(s) 173, 328
XapI RAATTY 2 cut(s) 438, 851
XbaI TCTAGA 3 cut(s) 613, 658, 691
XspI CTAG 5 cut(s) 270, 614, 659, 692, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.