Rorug04G0087000

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002666
Physical Location & Seq
Forward (+)
13486844 .. 13490908
4065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0087000.1

Sequence Viewer

Length: 1299 bp
ATGGAGACTGCCTGTCCAGAATCACCCAAACATTCCTTACCAGCTGAAACCATAGCCGACCACGACGACCTCCTCTCGCAGATCCTCGTCCGCTTAGAAGCCAAGTCCCTCATCCGCTACAAATCCGTCTCCAAGCACTGGCTCTCTCTCATTTCCAGCCCCAAATTCCGCGAACGCCACACCCTCCGAAACCCTAACCCTACCGTCTCCGCCTTCTTCTTCCTCTCCACCAACCAACGCCTCAGCTTCATCTCTCTCTCCGACGACGAAACTCGATGCGGATCCCACTCCAATCCGCTCGATTCGGTCCCGGAGCTGCGAGGAATCAAGATTCTGCAGTCCTGCAATGGCTTGCTCCTCTGCTGCTCCGGCGGAGAAGAAACGACCCGACGAGCATACTATGTGGTCAATCCCACAGCCAGTCAGTTCTCGAGACTTCCCTCTCCTCCATATGCTTGTCAATTCGATGATAGTTTGGGTATGGCTTTGGCTTTTGATCCTTATAGAACTGGAAATGTTCATTACAAAGTGGTCTCTGTTTGGATGACTAGTCGAAATACTATAGGGATTGGGATATATGCATCAGAGACTCGGAGTTGGAGTCTAGGCTTGAGTCAAGTCGACTTCAGGCAGTGCCAATTTGAAGTGGACTTCGGCCGCGGGGTGTACTGCAATGGAGCAATTCATTGGCTAAACCACAAGGATGAGTTGTTCCACTGTCACATTGGTGATGGCTCGGATGAAAAGCCGGTGAGGGGTGGAGTTTTGTCTAGTCCACTTGGTTTAATGTTTGATAGGGAGTATAGGTATTTTGGGGAGTCTTGTGGCCATCTGCACCTAATTGATATTTTCAAGCCTTGTGTTAATCAGTTTGATGTAAGGGAATTGGAGCCGGATTACTCTGGCTGGGTTGTCAAATACCATGTTGATCTTGATCCGATAATTGAAACTAATAGGCCTTGTATTAATGAGATGCTGATGCCCAAGGACTACTCTGGCTGGTTTGTCAAGTGCAAAGCTGATTTGTATTCCAGAAGCTATTCCTTTCAGGAGGTGGTTGGGTACTATTTGGATCGATATTATCAATTTGTTGTTCTATCTCTTACTCGAGAAGAGAATGGAGGGGATCTGTGTTTGTTGCTGTATTTTCGTGCTAAGGTCATCTCTTACAATCTTAGGGATAAGACGTTCAAGAAACTATGTGACTTAGCTCCGGAAGGCACCGCTGAGACCCAGAGATCTTTACAAGTTGAATGGCTGGAGGCTTATAATCATATGGGGAGTTTGGCTTGCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

432

Amino Acids

49.32

Weight (kDa)

6.07

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 58 3e-06 F-box domain
FBA_1 PF07734 97 - 234 2.8e-10 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 109 - 235 4.5e-07 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1269
AccB1I GGYRCC 1 cut(s) 1220
AccB7I CCANNNNNTGG 1 cut(s) 138
AccBSI CCGCTC 1 cut(s) 298
AccI GTMKAC 1 cut(s) 621
AccII CGCG 2 cut(s) 171, 660
AccIII TCCGGA 1 cut(s) 1213
AclWI GGATC 7 cut(s) 76, 276, 289, 491, 929, 1080, 1134
AcoI YGGCCR 2 cut(s) 655, 826
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 1 cut(s) 610
AfaI GTAC 2 cut(s) 668, 1064
AfiI CCNNNNNNNGG 2 cut(s) 138, 755
AgsI TTSAA 5 cut(s) 644, 853, 947, 1192, 1253
AhdI GACNNNNNGTC 1 cut(s) 12
AhlI ACTAGT 1 cut(s) 548
AleI CACNNNNGTG 1 cut(s) 726
AloI GAACNNNNNNTCC 4 cut(s) 695, 727, 1172, 1204
AluBI AGCT 6 cut(s) 44, 246, 316, 1019, 1038, 1211
AluI AGCT 6 cut(s) 44, 246, 316, 1019, 1038, 1211
Alw26I GTCTC 6 cut(s) 133, 211, 427, 538, 581, 1223
AlwI GGATC 7 cut(s) 76, 276, 289, 491, 929, 1080, 1134
Ama87I CYCGRG 2 cut(s) 430, 1107
Aor13HI TCCGGA 1 cut(s) 1213
AoxI GGCC 3 cut(s) 655, 826, 956
ApeKI GCWGC 2 cut(s) 316, 363
ApoI RAATTY 1 cut(s) 164
AseI ATTAAT 1 cut(s) 966
Asp700I GAANNNNTTC 1 cut(s) 1039
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 15, 740, 763
AvaI CYCGRG 2 cut(s) 430, 1107
AvaII GGWCC 1 cut(s) 307
BalI TGGCCA 1 cut(s) 828
BamHI GGATCC 1 cut(s) 281
BanI GGYRCC 1 cut(s) 1220
BbvCI CCTCAGC 1 cut(s) 242
BbvI GCAGC 2 cut(s) 303, 350
BccI CCATC 2 cut(s) 725, 837
BcnI CCSGG 1 cut(s) 311
BcoDI GTCTC 6 cut(s) 133, 211, 427, 538, 581, 1223
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 3 cut(s) 549, 605, 771
BfmI CTRYAG 2 cut(s) 335, 561
BglII AGATCT 1 cut(s) 1238
BisI GCNGC 3 cut(s) 317, 364, 658
BlsI GCNGC 3 cut(s) 318, 365, 659
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 307
BmeRI GACNNNNNGTC 1 cut(s) 12
BmeT110I CYCGRG 2 cut(s) 430, 1107
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 4 cut(s) 283, 309, 891, 1222
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 4 cut(s) 266, 590, 963, 969
BpmI CTGGAG 1 cut(s) 1280
Bpu10I CCTNAGC 2 cut(s) 242, 1155
BpuEI CTTGAG 1 cut(s) 631
BpuMI CCSGG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 1075
BsaBI GATNNNNATC 2 cut(s) 280, 933
BsaI GGTCTC 2 cut(s) 538, 1223
BsaJI CCNNGG 2 cut(s) 658, 984
BsaWI WCCGGW 1 cut(s) 1213
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 755
Bse118I RCCGGY 1 cut(s) 748
Bse1I ACTGG 3 cut(s) 143, 420, 514
Bse3DI GCAATG 2 cut(s) 352, 679
Bse8I GATNNNNATC 2 cut(s) 280, 933
BseAI TCCGGA 1 cut(s) 1213
BseCI ATCGAT 1 cut(s) 1075
BseDI CCNNGG 2 cut(s) 658, 984
BseGI GGATG 4 cut(s) 111, 549, 709, 745
BseJI GATNNNNATC 2 cut(s) 280, 933
BseLI CCNNNNNNNGG 2 cut(s) 138, 755
BseMI GCAATG 2 cut(s) 352, 679
BseMII CTCAG 2 cut(s) 256, 1218
BseNI ACTGG 3 cut(s) 143, 420, 514
BseRI GAGGAG 3 cut(s) 62, 347, 435
BseX3I CGGCCG 1 cut(s) 655
BseXI GCAGC 2 cut(s) 303, 350
BseYI CCCAGC 1 cut(s) 906
BsgI GTGCAG 1 cut(s) 818
Bsh1236I CGCG 2 cut(s) 171, 660
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 3 cut(s) 657, 828, 958
BshNI GGYRCC 1 cut(s) 1220
BshVI ATCGAT 1 cut(s) 1075
BsiEI CGRYCG 1 cut(s) 658
BsiHKCI CYCGRG 2 cut(s) 430, 1107
BsiSI CCGG 5 cut(s) 311, 369, 749, 893, 1214
BslFI GGGAC 2 cut(s) 91, 293
BslI CCNNNNNNNGG 2 cut(s) 138, 755
BsmAI GTCTC 6 cut(s) 133, 211, 427, 538, 581, 1223
BsmBI CGTCTC 2 cut(s) 133, 211
BsmFI GGGAC 2 cut(s) 91, 293
BsnI GGCC 3 cut(s) 657, 828, 958
Bso31I GGTCTC 2 cut(s) 538, 1223
BsoBI CYCGRG 2 cut(s) 430, 1107
Bsp13I TCCGGA 1 cut(s) 1213
Bsp143I GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
BspANI GGCC 3 cut(s) 657, 828, 958
BspCNI CTCAG 2 cut(s) 255, 1219
BspDI ATCGAT 1 cut(s) 1075
BspEI TCCGGA 1 cut(s) 1213
BspFNI CGCG 2 cut(s) 171, 660
BspLI GGNNCC 4 cut(s) 283, 309, 891, 1222
BspMAI CTGCAG 1 cut(s) 339
BspPI GGATC 7 cut(s) 76, 276, 289, 491, 929, 1080, 1134
BspT107I GGYRCC 1 cut(s) 1220
BspTNI GGTCTC 2 cut(s) 538, 1223
BsrBI CCGCTC 1 cut(s) 298
BsrDI GCAATG 2 cut(s) 352, 679
BsrFI RCCGGY 1 cut(s) 748
BsrI ACTGG 3 cut(s) 143, 420, 514
BssAI RCCGGY 1 cut(s) 748
BssECI CCNNGG 2 cut(s) 658, 984
BssMI GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
BssT1I CCWWGG 1 cut(s) 984
Bst4CI ACNGT 2 cut(s) 205, 719
Bst6I CTCTTC 1 cut(s) 1107
BstC8I GCNNGC 2 cut(s) 353, 1291
BstDEI CTNAG 6 cut(s) 94, 242, 1155, 1175, 1207, 1227
BstDSI CCRYGG 1 cut(s) 658
BstF5I GGATG 4 cut(s) 111, 549, 709, 745
BstFNI CGCG 2 cut(s) 171, 660
BstKTI GATC 8 cut(s) 84, 284, 499, 931, 937, 1075, 1129, 1241
BstMAI GTCTC 6 cut(s) 133, 211, 427, 538, 581, 1223
BstMBI GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 309
BstSFI CTRYAG 2 cut(s) 335, 561
BstUI CGCG 2 cut(s) 171, 660
BstV1I GCAGC 2 cut(s) 303, 350
BstX2I RGATCY 4 cut(s) 81, 281, 1126, 1238
BstYI RGATCY 4 cut(s) 81, 281, 1126, 1238
BstZI CGGCCG 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 1075
BsuRI GGCC 3 cut(s) 657, 828, 958
BsuTUI ATCGAT 1 cut(s) 1075
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 4 cut(s) 111, 549, 709, 745
BtsI GCAGTG 1 cut(s) 638
BtsIMutI CAGTG 3 cut(s) 136, 638, 715
Cac8I GCNNGC 2 cut(s) 353, 1291
Cfr10I RCCGGY 1 cut(s) 748
Cfr13I GGNCC 1 cut(s) 307
Cfr42I CCGCGG 1 cut(s) 661
ClaI ATCGAT 1 cut(s) 1075
Csp6I GTAC 2 cut(s) 667, 1063
CviAII CATG 1 cut(s) 923
CviQI GTAC 2 cut(s) 667, 1063
DdeI CTNAG 6 cut(s) 94, 242, 1155, 1175, 1207, 1227
DpnI GATC 8 cut(s) 83, 283, 498, 930, 936, 1074, 1128, 1240
DpnII GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
DriI GACNNNNNGTC 1 cut(s) 12
EaeI YGGCCR 2 cut(s) 655, 826
EagI CGGCCG 1 cut(s) 655
Eam1104I CTCTTC 1 cut(s) 1107
Eam1105I GACNNNNNGTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 1107
EciI GGCGGA 2 cut(s) 199, 387
EclXI CGGCCG 1 cut(s) 655
Eco130I CCWWGG 1 cut(s) 984
Eco147I AGGCCT 1 cut(s) 958
Eco31I GGTCTC 2 cut(s) 538, 1223
Eco47I GGWCC 1 cut(s) 307
Eco52I CGGCCG 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 610
Eco88I CYCGRG 2 cut(s) 430, 1107
EcoT14I CCWWGG 1 cut(s) 984
EcoT22I ATGCAT 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 984
Esp3I CGTCTC 2 cut(s) 133, 211
FaeI CATG 1 cut(s) 926
FaqI GGGAC 2 cut(s) 91, 293
FatI CATG 1 cut(s) 922
FauI CCCGC 1 cut(s) 653
FauNDI CATATG 2 cut(s) 451, 1275
FblI GTMKAC 1 cut(s) 621
Fnu4HI GCNGC 3 cut(s) 317, 364, 658
FokI GGATG 4 cut(s) 98, 556, 716, 752
Fsp4HI GCNGC 3 cut(s) 317, 364, 658
FspBI CTAG 3 cut(s) 549, 605, 771
GluI GCNGC 3 cut(s) 317, 364, 658
GsaI CCCAGC 1 cut(s) 910
GsuI CTGGAG 1 cut(s) 1280
HaeIII GGCC 3 cut(s) 657, 828, 958
HapII CCGG 5 cut(s) 311, 369, 749, 893, 1214
Hin1II CATG 1 cut(s) 926
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HinfI GANTC 8 cut(s) 20, 302, 324, 331, 589, 601, 613, 818
HpaII CCGG 5 cut(s) 311, 369, 749, 893, 1214
HphI GGTGA 3 cut(s) 15, 740, 763
Hpy166II GTNNAC 4 cut(s) 622, 649, 667, 776
Hpy188I TCNGA 6 cut(s) 188, 262, 586, 594, 739, 939
Hpy8I GTNNAC 4 cut(s) 622, 649, 667, 776
Hpy99I CGWCG 4 cut(s) 68, 266, 269, 393
HpyAV CCTTC 2 cut(s) 223, 1211
HpyCH4III ACNGT 2 cut(s) 205, 719
HpyCH4IV ACGT 1 cut(s) 1187
HpyCH4V TGCA 6 cut(s) 337, 345, 581, 672, 835, 1014
HpyF10VI GCNNNNNNNGC 1 cut(s) 369
HpyF3I CTNAG 6 cut(s) 94, 242, 1155, 1175, 1207, 1227
HpySE526I ACGT 1 cut(s) 1187
Hsp92II CATG 1 cut(s) 926
Kpn2I TCCGGA 1 cut(s) 1213
KspI CCGCGG 1 cut(s) 661
Kzo9I GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
LmnI GCTCC 6 cut(s) 313, 360, 371, 677, 889, 1216
Lsp1109I GCAGC 2 cut(s) 303, 350
LweI GCATC 4 cut(s) 266, 590, 963, 969
MaeI CTAG 3 cut(s) 549, 605, 771
MaeII ACGT 1 cut(s) 1187
MaeIII GTNAC 2 cut(s) 719, 1202
MalI GATC 8 cut(s) 83, 283, 498, 930, 936, 1074, 1128, 1240
MbiI CCGCTC 1 cut(s) 298
MboI GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
MboII GAAGA 4 cut(s) 208, 211, 389, 1124
MflI RGATCY 4 cut(s) 81, 281, 1126, 1238
MlsI TGGCCA 1 cut(s) 828
MluCI AATT 8 cut(s) 164, 461, 638, 681, 840, 884, 942, 1085
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 4 cut(s) 583, 610, 622, 827
MmeI TCCRAC 2 cut(s) 285, 578
Mox20I TGGCCA 1 cut(s) 828
Mph1103I ATGCAT 1 cut(s) 583
MroI TCCGGA 1 cut(s) 1213
MroXI GAANNNNTTC 1 cut(s) 1039
MscI TGGCCA 1 cut(s) 828
MseI TTAA 3 cut(s) 785, 864, 966
MslI CAYNNNNRTG 2 cut(s) 702, 726
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 3 cut(s) 44, 660, 1226
MspI CCGG 5 cut(s) 311, 369, 749, 893, 1214
MspR9I CCNGG 1 cut(s) 311
MvnI CGCG 2 cut(s) 171, 660
MwoI GCNNNNNNNGC 1 cut(s) 369
NciI CCSGG 1 cut(s) 311
NdeI CATATG 2 cut(s) 451, 1275
NdeII GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
NlaIII CATG 1 cut(s) 926
NlaIV GGNNCC 4 cut(s) 283, 309, 891, 1222
NmuCI GTSAC 2 cut(s) 719, 1202
NsiI ATGCAT 1 cut(s) 583
OliI CACNNNNGTG 1 cut(s) 726
PaeR7I CTCGAG 2 cut(s) 430, 1107
PceI AGGCCT 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 1039
PfeI GAWTC 4 cut(s) 20, 302, 324, 331
PflMI CCANNNNNTGG 1 cut(s) 138
PfoI TCCNGGA 1 cut(s) 309
PkrI GCNGC 3 cut(s) 318, 365, 659
PleI GAGTC 4 cut(s) 583, 609, 621, 826
PpsI GAGTC 4 cut(s) 583, 609, 621, 826
PshBI ATTAAT 1 cut(s) 966
PsiI TTATAA 1 cut(s) 1269
PspFI CCCAGC 1 cut(s) 906
PspN4I GGNNCC 4 cut(s) 283, 309, 891, 1222
PspPI GGNCC 1 cut(s) 307
PstI CTGCAG 1 cut(s) 339
PsuI RGATCY 4 cut(s) 81, 281, 1126, 1238
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 2 cut(s) 668, 1064
RsaNI GTAC 2 cut(s) 667, 1063
RseI CAYNNNNRTG 2 cut(s) 702, 726
SacII CCGCGG 1 cut(s) 661
SalI GTCGAC 1 cut(s) 620
SaqAI TTAA 3 cut(s) 785, 864, 966
SatI GCNGC 3 cut(s) 317, 364, 658
Sau3AI GATC 8 cut(s) 81, 281, 496, 928, 934, 1072, 1126, 1238
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 4 cut(s) 583, 610, 622, 827
ScrFI CCNGG 1 cut(s) 311
SfaNI GCATC 4 cut(s) 266, 590, 963, 969
SfcI CTRYAG 2 cut(s) 335, 561
Sfr274I CTCGAG 2 cut(s) 430, 1107
Sfr303I CCGCGG 1 cut(s) 661
SgrBI CCGCGG 1 cut(s) 661
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 2 cut(s) 430, 1107
SmiMI CAYNNNNRTG 2 cut(s) 702, 726
SmlI CTYRAG 3 cut(s) 430, 610, 1107
SmoI CTYRAG 3 cut(s) 430, 610, 1107
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 8 cut(s) 164, 461, 638, 681, 840, 884, 942, 1085
SseBI AGGCCT 1 cut(s) 958
SspMI CTAG 3 cut(s) 549, 605, 771
StuI AGGCCT 1 cut(s) 958
StyD4I CCNGG 1 cut(s) 309
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 2 cut(s) 205, 719
TaiI ACGT 1 cut(s) 1190
TaqI TCGA 8 cut(s) 274, 300, 431, 465, 553, 621, 1075, 1108
TaqII GACCGA 1 cut(s) 295
TasI AATT 8 cut(s) 164, 461, 638, 681, 840, 884, 942, 1085
TatI WGTACW 1 cut(s) 666
TauI GCSGC 1 cut(s) 660
TfiI GAWTC 4 cut(s) 20, 302, 324, 331
Tru1I TTAA 3 cut(s) 785, 864, 966
Tru9I TTAA 3 cut(s) 785, 864, 966
TscAI CASTG 3 cut(s) 143, 638, 722
TseFI GTSAC 2 cut(s) 719, 1202
TseI GCWGC 2 cut(s) 316, 363
Tsp45I GTSAC 2 cut(s) 719, 1202
TspDTI ATGAA 4 cut(s) 238, 509, 674, 756
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 3 cut(s) 143, 638, 722
Van91I CCANNNNNTGG 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 307
VspI ATTAAT 1 cut(s) 966
XapI RAATTY 1 cut(s) 164
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XhoI CTCGAG 2 cut(s) 430, 1107
XmiI GTMKAC 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 1039
XspI CTAG 3 cut(s) 549, 605, 771
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.