Rw4G012380

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002666
Physical Location & Seq
Reverse (-)
29136975 .. 29143660
6686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012380.1

Sequence Viewer

Length: 1404 bp
ATGGCAATTGCAGCTGCTTCTTCGCTTCCCTTGGAAGGAGTTTGTTGTTCGACAAGACATGGGATCTCAAGCAGTTGGATGAAACCTCCTTTTGATGGTCGAAGATCCCCCGATCCTCTGGGTGTAAGTTTGAAGTGCAGGAATCCGTTAGTTGGACCAACACAATTCCACTGGCTGTCTATTGGACATGGTCTCTGCCTTTCCAAAGTTTCAGTTGCTGCGGATTTCTCAGATTCTTCTCCTGAATCTTCTAGTTATATGACTAACCAGGGCTATCATCCTCTTGAGGAGTTGAAAGCGTGCAAAATGGTCCGAGACACTAAACTCACCTCTGCTGAAATAGCAAGACAACAGTCGAGGTCTCTGTTTTCTAGAGAAAGGCTGTTTACTAATTTTATAGGTAACGACAGTGCTTTGCTGGTTTTCCCTGGGAAGATACACTCTGAACCACATGAACAAATTTCTTGGGCTGAGTTTCAATATGTTATTGACGATTATGGAGATTTATATTTTGAACTGTTTGATGATGCGAACATATTAGAAGATCCTACAGCAAGTAACCCTGTGAATGCTTTGTTTGGAATGGATATCCCTGTACACAACAATGGAAGAATAACTGGAGGATTCAGCATTCTAGATGACCAGAACAGTGACGATATTCCTTTTGATGATGATTATCTAGAGGTTGTAGAATCTGAAGCTTTTGATGTTCTAGACTGGGAGATACCTGATGCTTCTGCCTTGATTCATCCCATTTATTTTGCAAAGTGCTTAACAAAGGCCATCAATATCAGACATGACAGAAAAATGGATCACCCATCAAATGGTGTTTCTATTTTAGGATGCCTCATACCTGCTTTTGCTGATGAAGAATTTTATGTAAGAAGACTATTTCATTATGAGGATAGCGACTATTACAACTCGGATGAGAAAGGTGGAAAAGGAGTGAGCATTAGTTCCAAAAGTGACCGAAGCAAGAATTGCTCAACACTCTACCGGTTGGAGATAATGAGAATAGAGTTGTTCTCGGTATATGGTGTTCAGTCCACAATTAACCTACAAGATTTTCAAGATGCGGAACCTGATTTTCTTATAAACTCTATCTCGGATATTGTAGAACGGTTCAACGAAAGAGGTATTAGATGCGACGTTGCTCTTAAAGCGTTATGCAAAAGAAAAGGACTTCAAGTTGAGGGAGCTCACTTGATCGGGGTTGACAGTCTAGGAATGGATGTTAGAGTCTTCTCAGGGTCAGAAGTACAAACTCATCGTTTTCCCTTCAGAGTCCGGGCCAAGTCTGAAGTTGCAGCTGAAAAACAGATTGAGCAACTTTTGTTCCCACGCTCTCGCCGTAAAAAGTTGAGAAGTCAAGGAAATAATTTTCAGGGTGTGGAGTCGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

467

Amino Acids

52.5

Weight (kDa)

5.27

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1094
Acc36I ACCTGC 1 cut(s) 862
AccB7I CCANNNNNTGG 1 cut(s) 824
AciI CCGC 2 cut(s) 221, 1076
AclWI GGATC 5 cut(s) 71, 99, 107, 539, 819
AcsI RAATTY 2 cut(s) 459, 872
AcuI CTGAAG 3 cut(s) 717, 1264, 1320
AfaI GTAC 3 cut(s) 597, 1260, 1400
AfiI CCNNNNNNNGG 4 cut(s) 35, 95, 152, 824
AgeI ACCGGT 1 cut(s) 996
AgsI TTSAA 7 cut(s) 133, 295, 479, 515, 1070, 1126, 1187
AjnI CCWGG 2 cut(s) 267, 427
AluBI AGCT 4 cut(s) 14, 701, 1199, 1310
AluI AGCT 4 cut(s) 14, 701, 1199, 1310
Alw21I GWGCWC 1 cut(s) 1201
Alw26I GTCTC 3 cut(s) 197, 309, 366
AlwI GGATC 5 cut(s) 71, 99, 107, 539, 819
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 2 cut(s) 780, 1290
ApeKI GCWGC 4 cut(s) 11, 14, 218, 1307
ApoI RAATTY 2 cut(s) 459, 872
AsiGI ACCGGT 1 cut(s) 996
AspS9I GGNCC 3 cut(s) 155, 310, 1290
AsuC2I CCSGG 1 cut(s) 1289
AsuHPI GGTGA 2 cut(s) 319, 806
AvaII GGWCC 2 cut(s) 155, 310
BanII GRGCYC 1 cut(s) 1201
BbsI GAAGAC 2 cut(s) 892, 1234
Bbv12I GWGCWC 1 cut(s) 1201
BbvI GCAGC 3 cut(s) 23, 205, 1319
BccI CCATC 3 cut(s) 89, 791, 826
BceAI ACGGC 1 cut(s) 1335
BciT130I CCWGG 2 cut(s) 269, 429
BcnI CCSGG 1 cut(s) 1289
BcoDI GTCTC 3 cut(s) 197, 309, 366
BfaI CTAG 6 cut(s) 252, 372, 635, 680, 713, 1223
BfmI CTRYAG 1 cut(s) 549
BfuAI ACCTGC 1 cut(s) 862
BisI GCNGC 4 cut(s) 12, 15, 219, 1308
BlsI GCNGC 4 cut(s) 13, 16, 220, 1309
Bme1390I CCNGG 3 cut(s) 269, 429, 1289
Bme18I GGWCC 2 cut(s) 155, 310
BmgT120I GGNCC 3 cut(s) 155, 310, 1290
BmiI GGNNCC 1 cut(s) 1080
BmrFI CCNGG 3 cut(s) 269, 429, 1289
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 5 cut(s) 517, 721, 833, 1063, 1133
BmuI ACTGGG 1 cut(s) 727
BoxI GACNNNNGTC 1 cut(s) 352
BpiI GAAGAC 2 cut(s) 892, 1234
BplI GAGNNNNNCTC 2 cut(s) 1010, 1042
BpmI CTGGAG 1 cut(s) 639
BpuEI CTTGAG 2 cut(s) 52, 305
BpuMI CCSGG 1 cut(s) 1289
BsaBI GATNNNNATC 1 cut(s) 675
BsaI GGTCTC 2 cut(s) 197, 366
BsaJI CCNNGG 4 cut(s) 30, 268, 427, 428
BsaWI WCCGGW 1 cut(s) 996
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 95, 152, 824
Bse118I RCCGGY 1 cut(s) 996
Bse1I ACTGG 3 cut(s) 176, 622, 722
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 2 cut(s) 269, 429
BseDI CCNNGG 4 cut(s) 30, 268, 427, 428
BseGI GGATG 6 cut(s) 84, 277, 748, 848, 931, 1237
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 4 cut(s) 35, 95, 152, 824
BseMII CTCAG 3 cut(s) 243, 462, 1260
BseNI ACTGG 3 cut(s) 176, 622, 722
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 3 cut(s) 23, 205, 1319
BsgI GTGCAG 1 cut(s) 157
BshFI GGCC 2 cut(s) 782, 1292
BshTI ACCGGT 1 cut(s) 996
BsiHKAI GWGCWC 1 cut(s) 1201
BsiSI CCGG 2 cut(s) 997, 1288
BslI CCNNNNNNNGG 4 cut(s) 35, 95, 152, 824
BsmAI GTCTC 3 cut(s) 197, 309, 366
BsmI GAATGC 2 cut(s) 574, 630
BsnI GGCC 2 cut(s) 782, 1292
Bso31I GGTCTC 2 cut(s) 197, 366
Bsp1286I GDGCHC 1 cut(s) 1201
Bsp1407I TGTACA 1 cut(s) 595
Bsp143I GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
BspACI CCGC 2 cut(s) 221, 1076
BspANI GGCC 2 cut(s) 782, 1292
BspCNI CTCAG 3 cut(s) 242, 463, 1259
BspLI GGNNCC 1 cut(s) 1080
BspMI ACCTGC 1 cut(s) 862
BspPI GGATC 5 cut(s) 71, 99, 107, 539, 819
BspTNI GGTCTC 2 cut(s) 197, 366
BsrFI RCCGGY 1 cut(s) 996
BsrGI TGTACA 1 cut(s) 595
BsrI ACTGG 3 cut(s) 176, 622, 722
BssAI RCCGGY 1 cut(s) 996
BssECI CCNNGG 4 cut(s) 30, 268, 427, 428
BssMI GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 269, 429
Bst4CI ACNGT 6 cut(s) 354, 410, 519, 650, 1122, 1220
BstAPI GCANNNNNTGC 1 cut(s) 981
BstAUI TGTACA 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 3 cut(s) 229, 471, 1246
BstF5I GGATG 6 cut(s) 84, 277, 748, 848, 931, 1237
BstKTI GATC 6 cut(s) 66, 107, 115, 547, 814, 1209
BstMAI GTCTC 3 cut(s) 197, 309, 366
BstMBI GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
BstMWI GCNNNNNNNGC 4 cut(s) 11, 341, 981, 1160
BstNI CCWGG 2 cut(s) 269, 429
BstPAI GACNNNNGTC 1 cut(s) 352
BstSCI CCNGG 3 cut(s) 267, 427, 1287
BstSFI CTRYAG 1 cut(s) 549
BstV1I GCAGC 3 cut(s) 23, 205, 1319
BstV2I GAAGAC 2 cut(s) 892, 1234
BstX2I RGATCY 3 cut(s) 63, 104, 544
BstYI RGATCY 3 cut(s) 63, 104, 544
BsuRI GGCC 2 cut(s) 782, 1292
BtsCI GGATG 6 cut(s) 84, 277, 748, 848, 931, 1237
BtsIMutI CAGTG 3 cut(s) 169, 415, 655
BveI ACCTGC 1 cut(s) 862
Cac8I GCNNGC 1 cut(s) 301
CaiI CAGNNNCTG 1 cut(s) 218
Cfr10I RCCGGY 1 cut(s) 996
Cfr13I GGNCC 3 cut(s) 155, 310, 1290
Csp6I GTAC 3 cut(s) 596, 1259, 1399
CspAI ACCGGT 1 cut(s) 996
CviAII CATG 4 cut(s) 59, 188, 452, 797
CviQI GTAC 3 cut(s) 596, 1259, 1399
DdeI CTNAG 3 cut(s) 229, 471, 1246
DpnI GATC 6 cut(s) 65, 106, 114, 546, 813, 1208
DpnII GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
Ecl136II GAGCTC 1 cut(s) 1199
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 1201
Eco31I GGTCTC 2 cut(s) 197, 366
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 2 cut(s) 155, 310
Eco53kI GAGCTC 1 cut(s) 1199
Eco57I CTGAAG 3 cut(s) 717, 1264, 1320
EcoICRI GAGCTC 1 cut(s) 1199
EcoRII CCWGG 2 cut(s) 267, 427
EcoRV GATATC 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 1201
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 4 cut(s) 62, 191, 455, 800
FatI CATG 4 cut(s) 58, 187, 451, 796
Fnu4HI GCNGC 4 cut(s) 12, 15, 219, 1308
FokI GGATG 6 cut(s) 91, 264, 735, 855, 938, 1244
FriOI GRGCYC 1 cut(s) 1201
Fsp4HI GCNGC 4 cut(s) 12, 15, 219, 1308
FspBI CTAG 6 cut(s) 252, 372, 635, 680, 713, 1223
GluI GCNGC 4 cut(s) 12, 15, 219, 1308
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 2 cut(s) 782, 1292
HapII CCGG 2 cut(s) 997, 1288
Hin1II CATG 4 cut(s) 62, 191, 455, 800
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HindIII AAGCTT 1 cut(s) 699
HinfI GANTC 9 cut(s) 142, 233, 245, 624, 692, 745, 1239, 1284, 1394
HpaII CCGG 2 cut(s) 997, 1288
HphI GGTGA 2 cut(s) 319, 806
Hpy166II GTNNAC 4 cut(s) 387, 598, 1047, 1216
Hpy188III TCNNGA 7 cut(s) 242, 284, 372, 635, 680, 713, 1070
Hpy8I GTNNAC 4 cut(s) 387, 598, 1047, 1216
Hpy99I CGWCG 1 cut(s) 1151
HpyAV CCTTC 2 cut(s) 29, 1288
HpyCH4III ACNGT 6 cut(s) 354, 410, 519, 650, 1122, 1220
HpyCH4IV ACGT 1 cut(s) 1149
HpyCH4V TGCA 6 cut(s) 11, 138, 303, 764, 1170, 1307
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 341, 981, 1160
HpyF3I CTNAG 3 cut(s) 229, 471, 1246
HpySE526I ACGT 1 cut(s) 1149
Hsp92II CATG 4 cut(s) 62, 191, 455, 800
Kzo9I GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
LmnI GCTCC 1 cut(s) 1196
Lsp1109I GCAGC 3 cut(s) 23, 205, 1319
LweI GCATC 5 cut(s) 517, 721, 833, 1063, 1133
MaeI CTAG 6 cut(s) 252, 372, 635, 680, 713, 1223
MaeII ACGT 1 cut(s) 1149
MaeIII GTNAC 4 cut(s) 401, 557, 650, 965
MalI GATC 6 cut(s) 65, 106, 114, 546, 813, 1208
MboI GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 3 cut(s) 63, 104, 544
MhlI GDGCHC 1 cut(s) 1201
MluCI AATT 8 cut(s) 6, 164, 391, 459, 872, 979, 1050, 1378
MlyI GAGTC 3 cut(s) 1248, 1293, 1403
MmeI TCCRAC 3 cut(s) 56, 133, 981
MseI TTAA 3 cut(s) 773, 1053, 1158
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 2 cut(s) 14, 1310
MspI CCGG 2 cut(s) 997, 1288
MspR9I CCNGG 3 cut(s) 269, 429, 1289
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 2 cut(s) 574, 630
MvaI CCWGG 2 cut(s) 269, 429
MwoI GCNNNNNNNGC 4 cut(s) 11, 341, 981, 1160
NciI CCSGG 1 cut(s) 1289
NdeII GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
NlaIII CATG 4 cut(s) 62, 191, 455, 800
NlaIV GGNNCC 1 cut(s) 1080
NmuCI GTSAC 2 cut(s) 650, 965
PasI CCCWGGG 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 1348
PctI GAATGC 2 cut(s) 574, 630
PfeI GAWTC 6 cut(s) 142, 233, 245, 624, 692, 745
PflFI GACNNNGTC 1 cut(s) 189
PflMI CCANNNNNTGG 1 cut(s) 824
PinAI ACCGGT 1 cut(s) 996
PkrI GCNGC 4 cut(s) 13, 16, 220, 1309
PleI GAGTC 3 cut(s) 1247, 1292, 1402
PpsI GAGTC 3 cut(s) 1247, 1292, 1402
PshAI GACNNNNGTC 1 cut(s) 352
PsiI TTATAA 1 cut(s) 1094
Psp124BI GAGCTC 1 cut(s) 1201
Psp6I CCWGG 2 cut(s) 267, 427
PspGI CCWGG 2 cut(s) 267, 427
PspN4I GGNNCC 1 cut(s) 1080
PspPI GGNCC 3 cut(s) 155, 310, 1290
PsrI GAACNNNNNNTAC 2 cut(s) 1023, 1055
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 3 cut(s) 63, 104, 544
PsyI GACNNNGTC 1 cut(s) 189
PvuII CAGCTG 2 cut(s) 14, 1310
RsaI GTAC 3 cut(s) 597, 1260, 1400
RsaNI GTAC 3 cut(s) 596, 1259, 1399
RseI CAYNNNNRTG 1 cut(s) 603
SacI GAGCTC 1 cut(s) 1201
SaqAI TTAA 3 cut(s) 773, 1053, 1158
SatI GCNGC 4 cut(s) 12, 15, 219, 1308
Sau3AI GATC 6 cut(s) 63, 104, 112, 544, 811, 1206
Sau96I GGNCC 3 cut(s) 155, 310, 1290
SchI GAGTC 3 cut(s) 1248, 1293, 1403
ScrFI CCNGG 3 cut(s) 269, 429, 1289
SduI GDGCHC 1 cut(s) 1201
SfaNI GCATC 5 cut(s) 517, 721, 833, 1063, 1133
SfcI CTRYAG 1 cut(s) 549
SinI GGWCC 2 cut(s) 155, 310
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 2 cut(s) 67, 284
SmoI CTYRAG 2 cut(s) 67, 284
Sse9I AATT 8 cut(s) 6, 164, 391, 459, 872, 979, 1050, 1378
SsiI CCGC 2 cut(s) 221, 1076
SspMI CTAG 6 cut(s) 252, 372, 635, 680, 713, 1223
SstI GAGCTC 1 cut(s) 1201
StyD4I CCNGG 3 cut(s) 267, 427, 1287
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 6 cut(s) 354, 410, 519, 650, 1122, 1220
TaiI ACGT 1 cut(s) 1152
TaqI TCGA 3 cut(s) 50, 100, 356
TaqII GACCGA 1 cut(s) 984
TasI AATT 8 cut(s) 6, 164, 391, 459, 872, 979, 1050, 1378
TatI WGTACW 2 cut(s) 595, 1258
TfiI GAWTC 6 cut(s) 142, 233, 245, 624, 692, 745
Tru1I TTAA 3 cut(s) 773, 1053, 1158
Tru9I TTAA 3 cut(s) 773, 1053, 1158
TscAI CASTG 3 cut(s) 176, 415, 655
TseFI GTSAC 2 cut(s) 650, 965
TseI GCWGC 4 cut(s) 11, 14, 218, 1307
Tsp45I GTSAC 2 cut(s) 650, 965
TspDTI ATGAA 5 cut(s) 95, 468, 737, 882, 884
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 3 cut(s) 176, 415, 655
Tth111I GACNNNGTC 1 cut(s) 189
Van91I CCANNNNNTGG 1 cut(s) 824
VpaK11BI GGWCC 2 cut(s) 155, 310
XapI RAATTY 2 cut(s) 459, 872
XbaI TCTAGA 4 cut(s) 371, 634, 679, 712
XspI CTAG 6 cut(s) 252, 372, 635, 680, 713, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.