AT3G60450

CONTAINS InterPro DOMAIN s Phosphoglycerate mutase (InterPro IPR013078), PRIB5 (InterPro IPR012398)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006635
Physical Location & Seq
Forward (+)
22340859 .. 22342330
1472 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60450.2

Sequence Viewer

Length: 825 bp
ATGGAGTCTCCCAAATCTAACGTCGACGATGGCTACCAAAACATCTTCGTCATGCGTCACGGTGATCGAATCGACAATTTCGAGCCACTCTGGGTTTCAACCGCCGAGAGACCTTGGGATCCACCTTTGGTTCAAGACGGTATGATTCGAGCGTTTCGAACCGGTCAAAGGATCCGATCTCAGATCGGGTTCCCGATTCACCGTGTCTTCGTCTCCCCTTTCCTCCGATGTCTTCAGACAGCTTCCGAAGTCGTCGCAGCTCTATCAGCCGTCAATGTCGATCCTAACGCCATGTCTTCCAAAGACGTCCCTTCCATTGATAAATCTAAGCTCAAGGTTTCTATTGAATTAGGTTTGTGTGAGATGTTGAACTCAGTGGCTATAAGGCGTGAGCTTGCTCCTAAAGATGGGAAATTTGACTTCACTGTTTCAGATATTGAAACTATGTTTCCTGAGGGAATGGTTGATCATAATGTGGATATGGTTTATAAAGAGTTGCCTAAATGGGAAGAGTCTGTGGAAGGTTGCAGAGATCGATATGTTAAAGTTGTAAAGGCTCTTGCTGATAAGTATCCTGAAGAGAACTTGCTCTTAGTAACCCACGGGGAAGGAGTTGGAACTACATTTTCGACATTCTATAAAGATACAACAGTGTACGAAGTAGACTACTGTGCTTATGTTGAAATGAGAAGACAAGTCTCAAGTGAAGATGGGTCTGTTAAAGCTGGGGACTATGAGGTGGTTTTGAGTCATGGTCAAGCTGGGATCAGGTTTAGTCATGATCCTGTCAGTTATGAACCTGTCATAAGCCATTCACCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

274

Amino Acids

30.86

Weight (kDa)

5.08

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 47 - 121 8.7e-06 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AatII GACGTC 1 cut(s) 309
AccI GTMKAC 2 cut(s) 24, 663
AciI CCGC 1 cut(s) 102
AclWI GGATC 7 cut(s) 113, 126, 166, 179, 275, 773, 776
AcsI RAATTY 1 cut(s) 413
AcuI CTGAAG 2 cut(s) 218, 597
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 1 cut(s) 656
AfiI CCNNNNNNNGG 2 cut(s) 168, 407
AgeI ACCGGT 1 cut(s) 161
AgsI TTSAA 6 cut(s) 99, 134, 347, 370, 440, 683
AluBI AGCT 6 cut(s) 242, 260, 331, 394, 725, 761
AluI AGCT 6 cut(s) 242, 260, 331, 394, 725, 761
Alw26I GTCTC 4 cut(s) 12, 103, 217, 703
AlwI GGATC 7 cut(s) 113, 126, 166, 179, 275, 773, 776
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 413
ArsI GACNNNNNNTTYG 1 cut(s) 38
AsiGI ACCGGT 1 cut(s) 161
AsuHPI GGTGA 3 cut(s) 74, 191, 807
AsuII TTCGAA 1 cut(s) 157
AxyI CCTNAGG 1 cut(s) 453
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BamHI GGATCC 2 cut(s) 118, 171
BbsI GAAGAC 4 cut(s) 199, 224, 288, 697
BbvI GCAGC 1 cut(s) 269
BccI CCATC 3 cut(s) 23, 401, 704
BceAI ACGGC 1 cut(s) 254
BciVI GTATCC 1 cut(s) 582
BclI TGATCA 1 cut(s) 466
BcoDI GTCTC 4 cut(s) 12, 103, 217, 703
BfuI GTATCC 1 cut(s) 582
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
BmiI GGNNCC 3 cut(s) 120, 173, 191
BpiI GAAGAC 4 cut(s) 199, 224, 288, 697
Bpu14I TTCGAA 1 cut(s) 157
BpuEI CTTGAG 2 cut(s) 317, 685
Bsa29I ATCGAT 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 2 cut(s) 113, 601
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 407
Bse118I RCCGGY 1 cut(s) 161
Bse21I CCTNAGG 1 cut(s) 453
Bse8I GATNNNNATC 1 cut(s) 570
BseCI ATCGAT 1 cut(s) 535
BseDI CCNNGG 2 cut(s) 113, 601
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 168, 407
BseMII CTCAG 3 cut(s) 194, 387, 444
BseXI GCAGC 1 cut(s) 269
BseYI CCCAGC 2 cut(s) 725, 761
BshTI ACCGGT 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 535
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 2 cut(s) 293, 743
BslI CCNNNNNNNGG 2 cut(s) 168, 407
BsmAI GTCTC 4 cut(s) 12, 103, 217, 703
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 2 cut(s) 293, 743
Bso31I GGTCTC 1 cut(s) 103
Bsp119I TTCGAA 1 cut(s) 157
BspACI CCGC 1 cut(s) 102
BspCNI CTCAG 3 cut(s) 193, 386, 445
BspDI ATCGAT 1 cut(s) 535
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 3 cut(s) 120, 173, 191
BspPI GGATC 7 cut(s) 113, 126, 166, 179, 275, 773, 776
BspT104I TTCGAA 1 cut(s) 157
BspTNI GGTCTC 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 113, 601
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 113
Bst4CI ACNGT 6 cut(s) 62, 140, 203, 427, 652, 671
Bst6I CTCTTC 2 cut(s) 504, 573
BstACI GRCGYC 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 157
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 5 cut(s) 180, 327, 373, 453, 592
BstDSI CCRYGG 1 cut(s) 601
BstMAI GTCTC 4 cut(s) 12, 103, 217, 703
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 269
BstV2I GAAGAC 4 cut(s) 199, 224, 288, 697
BstX2I RGATCY 2 cut(s) 118, 171
BstYI RGATCY 2 cut(s) 118, 171
Bsu15I ATCGAT 1 cut(s) 535
Bsu36I CCTNAGG 1 cut(s) 453
BsuI GTATCC 1 cut(s) 582
BsuTUI ATCGAT 1 cut(s) 535
BtgI CCRYGG 1 cut(s) 601
BtsIMutI CAGTG 3 cut(s) 381, 423, 657
Cac8I GCNNGC 1 cut(s) 396
CciI TCATGA 1 cut(s) 778
Cfr10I RCCGGY 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 535
CseI GACGC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 655
CspAI ACCGGT 1 cut(s) 161
CviAII CATG 4 cut(s) 52, 292, 752, 779
CviQI GTAC 1 cut(s) 655
DdeI CTNAG 5 cut(s) 180, 327, 373, 453, 592
Eam1104I CTCTTC 2 cut(s) 504, 573
EarI CTCTTC 2 cut(s) 504, 573
Eco130I CCWWGG 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 103
Eco57I CTGAAG 2 cut(s) 218, 597
Eco81I CCTNAGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 113
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 4 cut(s) 55, 295, 755, 782
FaqI GGGAC 2 cut(s) 293, 743
FatI CATG 4 cut(s) 51, 291, 751, 778
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 2 cut(s) 24, 663
Fnu4HI GCNGC 1 cut(s) 258
Fsp4HI GCNGC 1 cut(s) 258
GluI GCNGC 1 cut(s) 258
GsaI CCCAGC 2 cut(s) 729, 765
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 4 cut(s) 55, 295, 755, 782
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 6 cut(s) 5, 69, 145, 196, 512, 748
HpaII CCGG 1 cut(s) 162
HphI GGTGA 3 cut(s) 74, 191, 807
Hpy166II GTNNAC 3 cut(s) 25, 655, 664
Hpy188I TCNGA 6 cut(s) 176, 183, 227, 237, 247, 433
Hpy188III TCNNGA 5 cut(s) 134, 193, 452, 575, 779
Hpy8I GTNNAC 3 cut(s) 25, 655, 664
Hpy99I CGWCG 3 cut(s) 26, 29, 257
HpyAV CCTTC 3 cut(s) 321, 515, 602
HpyCH4III ACNGT 6 cut(s) 62, 140, 203, 427, 652, 671
HpyCH4IV ACGT 2 cut(s) 21, 306
HpyCH4V TGCA 1 cut(s) 528
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 5 cut(s) 180, 327, 373, 453, 592
HpySE526I ACGT 2 cut(s) 21, 306
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 4 cut(s) 55, 295, 755, 782
Ksp22I TGATCA 1 cut(s) 466
LmnI GCTCC 1 cut(s) 403
LpnPI CCDG 9 cut(s) 76, 175, 465, 588, 711, 747, 754, 798, 813
Lsp1109I GCAGC 1 cut(s) 269
MaeII ACGT 2 cut(s) 21, 306
MaeIII GTNAC 2 cut(s) 56, 595
MboII GAAGA 8 cut(s) 37, 199, 224, 288, 521, 590, 702, 719
MflI RGATCY 2 cut(s) 118, 171
MluCI AATT 3 cut(s) 76, 347, 413
MlyI GAGTC 3 cut(s) 14, 521, 757
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 3 cut(s) 233, 448, 730
MseI TTAA 2 cut(s) 543, 720
MspI CCGG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 266
NlaIII CATG 4 cut(s) 55, 295, 755, 782
NlaIV GGNNCC 3 cut(s) 120, 173, 191
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 56
NspV TTCGAA 1 cut(s) 157
PagI TCATGA 1 cut(s) 778
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 154
PfeI GAWTC 3 cut(s) 69, 145, 196
PinAI ACCGGT 1 cut(s) 161
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 3 cut(s) 13, 520, 756
PpsI GAGTC 3 cut(s) 13, 520, 756
PsiI TTATAA 1 cut(s) 489
PspFI CCCAGC 2 cut(s) 725, 761
PspN4I GGNNCC 3 cut(s) 120, 173, 191
PsuI RGATCY 2 cut(s) 118, 171
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
SalI GTCGAC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 543, 720
SatI GCNGC 1 cut(s) 258
SchI GAGTC 3 cut(s) 14, 521, 757
SfuI TTCGAA 1 cut(s) 157
SgrDI CGTCGACG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 332, 700
SmoI CTYRAG 2 cut(s) 332, 700
Sse9I AATT 3 cut(s) 76, 347, 413
SsiI CCGC 1 cut(s) 102
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 6 cut(s) 62, 140, 203, 427, 652, 671
TaiI ACGT 2 cut(s) 24, 309
TaqI TCGA 9 cut(s) 24, 67, 72, 81, 148, 157, 279, 535, 629
TasI AATT 3 cut(s) 76, 347, 413
TfiI GAWTC 3 cut(s) 69, 145, 196
Tru1I TTAA 2 cut(s) 543, 720
Tru9I TTAA 2 cut(s) 543, 720
TscAI CASTG 3 cut(s) 381, 430, 657
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 810
TspRI CASTG 3 cut(s) 381, 430, 657
XapI RAATTY 1 cut(s) 413
XmiI GTMKAC 2 cut(s) 24, 663
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.