MD07G1100900.v1.1
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006635
Physical Location & Seq
Forward (+)
11186264 .. 11188711
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1100900.v1.1.491

Sequence Viewer

Length: 969 bp
ATGTGGTTATCGAAACTTACAAAACTAACGCCCATGCTCTCCTCTTCCTTCTCAACTCAATCATTCCCAAGAAAAGCTCTCTTCGTTTCTGCCAACTCTGCTTCCATCCGGCTTCGACCTCTCTCTCAAAACTTACCCGAAATGGACGCGGACAAAAGTCAATCCAAATCGATCAGCGAAGCCTCCGATCGGCCCACGGACCACCGCTTCCAAAACGTCGTCGTCATGCGCCACGGCGACCGCATCGACAACTTCGAGCCCCTCTGGACATCGCAATCGCCGAGGCCGTGGGACCCGCCTCTGGTCGAAGAGGGCAAGGTCCGGGCCTTCTGCACCGGTACCAAGCTCCGATCGGGTCTCGGGTTTCCAATCCACCGCGTTTTCGTCTCGCCATTCGTGCGATGCGTCGAGACCGCCGTCCAAGTCGTCACCGCCCTCTCCGCCACCGAAGACCCGTCTCTCTCCAAATACGAGAGCGATCAGCCCGTCCCAGTCGACCCCTCTAAGCTCAAGGTATCAATTGAATATGGGTTAAGTGAGATGCTAAACAAAGAGGCGATTCGCGGTGATTTGGCTCCGAAAGATGGACAATGGGGCTTCAACATTGCAGATCTTGAGGCTATGTTCCCGGCTGGGACAGTGGATCAAACTGCGGAACGGGTGTACAAAGAGCTGCCTCAGTGGGGGGAGAGCGTGACGGGCGCCCGGGCTAGATATGTTCATGTCATTGAGGCTCTTGCTGACAAATACCCTACAGAAAACTTGCTCCTTGTCAGTCATGGTGAAGGAGTTGGTTCTTCAATTTCTGCCTTCCTGGAAGGCGCCACAGTATACGAAGTAGAATACTGCGCATATTCAGAACTAAGAAGGCCCATAAAAAACCGGTCATCAGCTGCAGGAAAGTTTGAGGTAATGGTAAATCGTGGCCGGACTGGTGTCAGTTACTCACTCCCAACCTCTGGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

323

Amino Acids

35.57

Weight (kDa)

6.55

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 215 - 272 8.5e-06 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 850
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 3 cut(s) 338, 701, 821
AccB7I CCANNNNNTGG 1 cut(s) 959
AccI GTMKAC 2 cut(s) 495, 831
AccII CGCG 3 cut(s) 149, 378, 564
AclWI GGATC 1 cut(s) 651
AcoI YGGCCR 1 cut(s) 925
AcyI GRCGYC 2 cut(s) 702, 822
AfaI GTAC 2 cut(s) 340, 665
AfiI CCNNNNNNNGG 5 cut(s) 189, 301, 584, 683, 959
AgeI ACCGGT 2 cut(s) 335, 882
AgsI TTSAA 3 cut(s) 524, 601, 801
AjnI CCWGG 1 cut(s) 813
AluBI AGCT 5 cut(s) 77, 346, 508, 673, 893
AluI AGCT 5 cut(s) 77, 346, 508, 673, 893
Alw26I GTCTC 4 cut(s) 362, 391, 404, 462
AlwI GGATC 1 cut(s) 651
Ama87I CYCGRG 2 cut(s) 359, 705
AoxI GGCC 5 cut(s) 191, 284, 324, 869, 925
ApeKI GCWGC 2 cut(s) 673, 893
AsiGI ACCGGT 2 cut(s) 335, 882
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 4 cut(s) 231, 704, 824, 851
AspS9I GGNCC 6 cut(s) 192, 199, 292, 319, 324, 870
AsuC2I CCSGG 4 cut(s) 323, 629, 706, 707
AsuHPI GGTGA 3 cut(s) 421, 578, 794
AvaI CYCGRG 2 cut(s) 359, 705
AvaII GGWCC 3 cut(s) 199, 292, 319
BanI GGYRCC 3 cut(s) 338, 701, 821
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 456
BbvI GCAGC 2 cut(s) 660, 880
BccI CCATC 2 cut(s) 113, 578
BceAI ACGGC 3 cut(s) 250, 271, 401
BciT130I CCWGG 1 cut(s) 815
BcnI CCSGG 4 cut(s) 323, 629, 706, 707
BcoDI GTCTC 4 cut(s) 362, 391, 404, 462
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 2 cut(s) 753, 894
BfoI RGCGCY 2 cut(s) 705, 825
BglII AGATCT 1 cut(s) 610
BisI GCNGC 2 cut(s) 674, 894
BlsI GCNGC 2 cut(s) 675, 895
Bme1390I CCNGG 5 cut(s) 323, 629, 706, 707, 815
Bme18I GGWCC 3 cut(s) 199, 292, 319
BmeT110I CYCGRG 2 cut(s) 359, 705
BmgT120I GGNCC 6 cut(s) 192, 199, 292, 319, 324, 870
BmiI GGNNCC 6 cut(s) 293, 294, 340, 576, 703, 823
BmrFI CCNGG 5 cut(s) 323, 629, 706, 707, 815
BmrI ACTGGG 1 cut(s) 485
BmsI GCATC 3 cut(s) 252, 392, 531
BmuI ACTGGG 1 cut(s) 485
BoxI GACNNNNGTC 3 cut(s) 156, 416, 935
BpiI GAAGAC 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 494, 635
BpuMI CCSGG 4 cut(s) 323, 629, 706, 707
Bsa29I ATCGAT 1 cut(s) 170
BsaHI GRCGYC 2 cut(s) 702, 822
BsaI GGTCTC 2 cut(s) 362, 404
BsaJI CCNNGG 5 cut(s) 195, 232, 281, 287, 705
BsaWI WCCGGW 2 cut(s) 335, 882
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 301, 584, 683, 959
Bse118I RCCGGY 2 cut(s) 335, 882
Bse1I ACTGG 2 cut(s) 491, 937
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 1 cut(s) 815
BseCI ATCGAT 1 cut(s) 170
BseDI CCNNGG 5 cut(s) 195, 232, 281, 287, 705
BseGI GGATG 1 cut(s) 105
BseLI CCNNNNNNNGG 5 cut(s) 189, 301, 584, 683, 959
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 1 cut(s) 692
BseNI ACTGG 2 cut(s) 491, 937
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 2 cut(s) 660, 880
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 316
Bsh1236I CGCG 3 cut(s) 149, 378, 564
Bsh1285I CGRYCG 3 cut(s) 190, 241, 353
BshFI GGCC 5 cut(s) 193, 286, 326, 871, 927
BshNI GGYRCC 3 cut(s) 338, 701, 821
BshTI ACCGGT 2 cut(s) 335, 882
BshVI ATCGAT 1 cut(s) 170
BsiEI CGRYCG 3 cut(s) 190, 241, 353
BsiHKCI CYCGRG 2 cut(s) 359, 705
BsiSI CCGG 7 cut(s) 109, 322, 336, 629, 706, 883, 928
BslFI GGGAC 3 cut(s) 305, 473, 649
BslI CCNNNNNNNGG 5 cut(s) 189, 301, 584, 683, 959
BsmAI GTCTC 4 cut(s) 362, 391, 404, 462
BsmBI CGTCTC 2 cut(s) 391, 462
BsmFI GGGAC 3 cut(s) 305, 473, 649
BsnI GGCC 5 cut(s) 193, 286, 326, 871, 927
Bso31I GGTCTC 2 cut(s) 362, 404
BsoBI CYCGRG 2 cut(s) 359, 705
Bsp1286I GDGCHC 1 cut(s) 261
Bsp1407I TGTACA 1 cut(s) 663
Bsp143I GATC 6 cut(s) 171, 187, 350, 478, 610, 643
BspANI GGCC 5 cut(s) 193, 286, 326, 871, 927
BspCNI CTCAG 1 cut(s) 691
BspDI ATCGAT 1 cut(s) 170
BspFNI CGCG 3 cut(s) 149, 378, 564
BspLI GGNNCC 6 cut(s) 293, 294, 340, 576, 703, 823
BspMAI CTGCAG 1 cut(s) 898
BspPI GGATC 1 cut(s) 651
BspT107I GGYRCC 3 cut(s) 338, 701, 821
BspTNI GGTCTC 2 cut(s) 362, 404
BsrDI GCAATG 1 cut(s) 603
BsrFI RCCGGY 2 cut(s) 335, 882
BsrGI TGTACA 1 cut(s) 663
BsrI ACTGG 2 cut(s) 491, 937
BssAI RCCGGY 2 cut(s) 335, 882
BssECI CCNNGG 5 cut(s) 195, 232, 281, 287, 705
BssMI GATC 6 cut(s) 171, 187, 350, 478, 610, 643
BssNAI GTATAC 1 cut(s) 832
BssNI GRCGYC 2 cut(s) 702, 822
Bst1107I GTATAC 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 815
Bst4CI ACNGT 2 cut(s) 640, 829
Bst6I CTCTTC 3 cut(s) 49, 86, 303
BstACI GRCGYC 2 cut(s) 702, 822
BstAUI TGTACA 1 cut(s) 663
BstDEI CTNAG 3 cut(s) 504, 678, 863
BstDSI CCRYGG 3 cut(s) 195, 232, 287
BstF5I GGATG 1 cut(s) 105
BstFNI CGCG 3 cut(s) 149, 378, 564
BstH2I RGCGCY 2 cut(s) 705, 825
BstHHI GCGC 4 cut(s) 231, 704, 824, 851
BstKTI GATC 6 cut(s) 174, 190, 353, 481, 613, 646
BstMAI GTCTC 4 cut(s) 362, 391, 404, 462
BstMBI GATC 6 cut(s) 171, 187, 350, 478, 610, 643
BstMCI CGRYCG 3 cut(s) 190, 241, 353
BstMWI GCNNNNNNNGC 4 cut(s) 98, 397, 440, 699
BstNI CCWGG 1 cut(s) 815
BstPAI GACNNNNGTC 3 cut(s) 156, 416, 935
BstSCI CCNGG 5 cut(s) 321, 627, 704, 705, 813
BstSFI CTRYAG 2 cut(s) 753, 894
BstUI CGCG 3 cut(s) 149, 378, 564
BstV1I GCAGC 2 cut(s) 660, 880
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BstZ17I GTATAC 1 cut(s) 832
Bsu15I ATCGAT 1 cut(s) 170
BsuRI GGCC 5 cut(s) 193, 286, 326, 871, 927
BsuTUI ATCGAT 1 cut(s) 170
BtgI CCRYGG 3 cut(s) 195, 232, 287
BtgZI GCGATG 2 cut(s) 255, 415
BtsCI GGATG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 645, 686
CfoI GCGC 4 cut(s) 231, 704, 824, 851
Cfr10I RCCGGY 2 cut(s) 335, 882
Cfr13I GGNCC 6 cut(s) 192, 199, 292, 319, 324, 870
Cfr9I CCCGGG 1 cut(s) 705
ClaI ATCGAT 1 cut(s) 170
CseI GACGC 2 cut(s) 155, 394
Csp6I GTAC 2 cut(s) 339, 664
CspAI ACCGGT 2 cut(s) 335, 882
CviAII CATG 4 cut(s) 34, 226, 722, 779
CviQI GTAC 2 cut(s) 339, 664
DdeI CTNAG 3 cut(s) 504, 678, 863
DinI GGCGCC 2 cut(s) 703, 823
DpnI GATC 6 cut(s) 173, 189, 352, 480, 612, 645
DpnII GATC 6 cut(s) 171, 187, 350, 478, 610, 643
EaeI YGGCCR 1 cut(s) 925
Eam1104I CTCTTC 3 cut(s) 49, 86, 303
EarI CTCTTC 3 cut(s) 49, 86, 303
EciI GGCGGA 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 2 cut(s) 362, 404
Eco47I GGWCC 3 cut(s) 199, 292, 319
Eco88I CYCGRG 2 cut(s) 359, 705
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 813
EcoT38I GRGCYC 1 cut(s) 261
EgeI GGCGCC 2 cut(s) 703, 823
EheI GGCGCC 2 cut(s) 703, 823
Esp3I CGTCTC 2 cut(s) 391, 462
FaeI CATG 4 cut(s) 37, 229, 725, 782
FaqI GGGAC 3 cut(s) 305, 473, 649
FatI CATG 4 cut(s) 33, 225, 721, 778
FauI CCCGC 1 cut(s) 303
FblI GTMKAC 2 cut(s) 495, 831
Fnu4HI GCNGC 2 cut(s) 674, 894
FokI GGATG 1 cut(s) 92
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 2 cut(s) 674, 894
FspBI CTAG 1 cut(s) 711
FspI TGCGCA 1 cut(s) 850
GlaI GCGC 4 cut(s) 230, 703, 823, 850
GluI GCNGC 2 cut(s) 674, 894
GsaI CCCAGC 1 cut(s) 636
HaeII RGCGCY 2 cut(s) 705, 825
HaeIII GGCC 5 cut(s) 193, 286, 326, 871, 927
HapII CCGG 7 cut(s) 109, 322, 336, 629, 706, 883, 928
HgaI GACGC 2 cut(s) 155, 394
HhaI GCGC 4 cut(s) 231, 704, 824, 851
Hin1I GRCGYC 2 cut(s) 702, 822
Hin1II CATG 4 cut(s) 37, 229, 725, 782
Hin6I GCGC 4 cut(s) 229, 702, 822, 849
HinP1I GCGC 4 cut(s) 229, 702, 822, 849
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 1 cut(s) 559
HpaII CCGG 7 cut(s) 109, 322, 336, 629, 706, 883, 928
HphI GGTGA 3 cut(s) 421, 578, 794
Hpy166II GTNNAC 3 cut(s) 496, 664, 832
Hpy188I TCNGA 4 cut(s) 187, 350, 579, 859
Hpy188III TCNNGA 4 cut(s) 265, 409, 614, 960
Hpy8I GTNNAC 3 cut(s) 496, 664, 832
Hpy99I CGWCG 3 cut(s) 221, 224, 410
HpyAV CCTTC 6 cut(s) 58, 337, 779, 812, 820, 861
HpyCH4III ACNGT 2 cut(s) 640, 829
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 3 cut(s) 333, 608, 896
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 397, 440, 699
HpyF3I CTNAG 3 cut(s) 504, 678, 863
HpySE526I ACGT 1 cut(s) 216
Hsp92I GRCGYC 2 cut(s) 702, 822
Hsp92II CATG 4 cut(s) 37, 229, 725, 782
HspAI GCGC 4 cut(s) 229, 702, 822, 849
KasI GGCGCC 2 cut(s) 701, 821
KflI GGGWCCC 1 cut(s) 292
KpnI GGTACC 1 cut(s) 342
Kzo9I GATC 6 cut(s) 171, 187, 350, 478, 610, 643
LmnI GCTCC 3 cut(s) 351, 580, 771
Lsp1109I GCAGC 2 cut(s) 660, 880
LweI GCATC 3 cut(s) 252, 392, 531
MaeI CTAG 1 cut(s) 711
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 3 cut(s) 427, 694, 941
MalI GATC 6 cut(s) 173, 189, 352, 480, 612, 645
MboI GATC 6 cut(s) 171, 187, 350, 478, 610, 643
MboII GAAGA 5 cut(s) 36, 73, 320, 461, 789
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 2 cut(s) 519, 801
Mly113I GGCGCC 2 cut(s) 702, 822
MseI TTAA 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 893
MspI CCGG 7 cut(s) 109, 322, 336, 629, 706, 883, 928
MspR9I CCNGG 5 cut(s) 323, 629, 706, 707, 815
MunI CAATTG 1 cut(s) 519
MvaI CCWGG 1 cut(s) 815
MvnI CGCG 3 cut(s) 149, 378, 564
MwoI GCNNNNNNNGC 4 cut(s) 98, 397, 440, 699
NarI GGCGCC 2 cut(s) 702, 822
NciI CCSGG 4 cut(s) 323, 629, 706, 707
NdeII GATC 6 cut(s) 171, 187, 350, 478, 610, 643
NlaIII CATG 4 cut(s) 37, 229, 725, 782
NlaIV GGNNCC 6 cut(s) 293, 294, 340, 576, 703, 823
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 2 cut(s) 427, 694
NsbI TGCGCA 1 cut(s) 850
PcsI WCGNNNNNNNCGW 5 cut(s) 252, 278, 284, 402, 414
PfeI GAWTC 1 cut(s) 559
PflMI CCANNNNNTGG 1 cut(s) 959
PfoI TCCNGGA 1 cut(s) 813
PinAI ACCGGT 2 cut(s) 335, 882
PkrI GCNGC 2 cut(s) 675, 895
Ple19I CGATCG 2 cut(s) 190, 353
PluTI GGCGCC 2 cut(s) 705, 825
PpuMI RGGWCCY 1 cut(s) 292
PshAI GACNNNNGTC 3 cut(s) 156, 416, 935
Psp5II RGGWCCY 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 813
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 1 cut(s) 813
PspN4I GGNNCC 6 cut(s) 293, 294, 340, 576, 703, 823
PspPI GGNCC 6 cut(s) 192, 199, 292, 319, 324, 870
PspPPI RGGWCCY 1 cut(s) 292
PstI CTGCAG 1 cut(s) 898
PsuI RGATCY 1 cut(s) 610
PvuI CGATCG 2 cut(s) 190, 353
PvuII CAGCTG 1 cut(s) 893
RsaI GTAC 2 cut(s) 340, 665
RsaNI GTAC 2 cut(s) 339, 664
SalI GTCGAC 1 cut(s) 494
SaqAI TTAA 1 cut(s) 533
SatI GCNGC 2 cut(s) 674, 894
Sau3AI GATC 6 cut(s) 171, 187, 350, 478, 610, 643
Sau96I GGNCC 6 cut(s) 192, 199, 292, 319, 324, 870
ScrFI CCNGG 5 cut(s) 323, 629, 706, 707, 815
SduI GDGCHC 1 cut(s) 261
SfaNI GCATC 3 cut(s) 252, 392, 531
SfcI CTRYAG 2 cut(s) 753, 894
SfoI GGCGCC 2 cut(s) 703, 823
SinI GGWCC 3 cut(s) 199, 292, 319
SmaI CCCGGG 1 cut(s) 707
SmlI CTYRAG 2 cut(s) 509, 614
SmoI CTYRAG 2 cut(s) 509, 614
SrfI GCCCGGGC 1 cut(s) 707
Sse9I AATT 2 cut(s) 519, 801
SspDI GGCGCC 2 cut(s) 701, 821
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 5 cut(s) 321, 627, 704, 705, 813
TaaI ACNGT 2 cut(s) 640, 829
TaiI ACGT 1 cut(s) 219
TaqI TCGA 8 cut(s) 11, 115, 170, 246, 255, 306, 408, 495
TasI AATT 2 cut(s) 519, 801
TatI WGTACW 1 cut(s) 663
TfiI GAWTC 1 cut(s) 559
Tru1I TTAA 1 cut(s) 533
Tru9I TTAA 1 cut(s) 533
TscAI CASTG 2 cut(s) 645, 686
TseFI GTSAC 2 cut(s) 427, 694
TseI GCWGC 2 cut(s) 673, 893
Tsp45I GTSAC 2 cut(s) 427, 694
TspDTI ATGAA 1 cut(s) 710
TspGWI ACGGA 1 cut(s) 212
TspMI CCCGGG 1 cut(s) 705
TspRI CASTG 2 cut(s) 645, 686
Van91I CCANNNNNTGG 1 cut(s) 959
VpaK11BI GGWCC 3 cut(s) 199, 292, 319
XmaI CCCGGG 1 cut(s) 705
XmiI GTMKAC 2 cut(s) 495, 831
XspI CTAG 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.